FvH4_2g25950

Inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015173
Physical Location & Seq
Forward (+)
20914273 .. 20915258
986 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25950.t1

Sequence Viewer

Length: 597 bp
ATGAAGCCAATCACATCATTCTTCTTCCTCTCAACACTCTGCGTGCTCCAACATGTTGCCCTCGCTGCAAATAGCAAACCTGTAGGCGGCCAACCGGGCAAACCTGCCAGAGGCCAACCGTCCGGTCCCGTCAGTGGGAATCTACTAGAAGAAGCATGCAAACAAGCGACGAACAAGGACCTATGCATCAACAGCCTGAAGAAAGACCCTAACAGCCAAGGAGCCGACCTCACGGGCCTGGCCTTCATAGCGCTCAGGCTGGCCGCCGCGAATGCCGCCGACGTTGAATCCCACCTGAGGACGCTGATGCTCAAAAACGCCACTGGTCTAGACCCGGATGTTCAGCAGGGGGTTCAGGATTGCATAGACAGTTATACCGACGCCAACGAGCAGCTGGATGACTCGGTGGCCGCGCTGTCGGACAAGAAGTGCAAAGACGTCGTGGTCTGGGTGAAGGTAGCCATTGCCGACTCGAATTATTGTGAAAGCTCCCTAGACGGTGAGCCTACTGTGCTTGCTGACAAGAACAAAGTGTTTATACAGATGTGCGAGAATGTCTTGTCCGTGATCAAGGCTTTGGCCCCAAAGGGTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

199

Amino Acids

20.93

Weight (kDa)

5.17

Isoelectric Point (pI)

26.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 48 - 188 1e-22 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55770 AT4G02250
fragaria_vesca FvH4_2g25950
malus_domestica MD03G1290800.v1.1 MD11G1307600.v1.1
rosa_chinensis RchiOBHm_Chr6g0294271
rosa_laevigata RLG00000011882
rosa_multiflora Rmu_co8483625.1_g000001 Rmu_sc0003996.1_g000007
rosa_roxburghii Rroxscaffold_7G00173490
rosa_rugosa Rorug06G0243400
rosa_samantha Rh6AG355600 Rh6BG362500 Rh6CG369600 Rh6DG356400
rosa_wichuraiana Rw6G030980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 443
AatII GACGTC 1 cut(s) 441
Acc36I ACCTGC 1 cut(s) 112
AccII CGCG 2 cut(s) 269, 413
AciI CCGC 5 cut(s) 87, 264, 267, 276, 411
AcoI YGGCCR 3 cut(s) 88, 261, 408
AcuI CTGAAG 1 cut(s) 218
AcyI GRCGYC 2 cut(s) 381, 438
AfeI AGCGCT 1 cut(s) 252
AfiI CCNNNNNNNGG 5 cut(s) 86, 110, 134, 135, 297
AflIII ACRYGT 1 cut(s) 52
AgsI TTSAA 1 cut(s) 287
AjnI CCWGG 1 cut(s) 237
AluBI AGCT 2 cut(s) 394, 489
AluI AGCT 2 cut(s) 394, 489
Alw21I GWGCWC 1 cut(s) 48
Aor51HI AGCGCT 1 cut(s) 252
AoxI GGCC 7 cut(s) 88, 112, 235, 240, 261, 408, 579
ApeKI GCWGC 2 cut(s) 65, 391
AspLEI GCGC 2 cut(s) 253, 415
AspS9I GGNCC 4 cut(s) 125, 178, 235, 580
AsuC2I CCSGG 2 cut(s) 96, 335
AsuHPI GGTGA 2 cut(s) 463, 512
AvaII GGWCC 2 cut(s) 125, 178
AxyI CCTNAGG 1 cut(s) 296
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 2 cut(s) 52, 403
BcgI CGANNNNNNTGC 2 cut(s) 421, 455
BciT130I CCWGG 1 cut(s) 239
BclI TGATCA 1 cut(s) 567
BcnI CCSGG 2 cut(s) 96, 335
BfaI CTAG 3 cut(s) 146, 329, 494
BfmI CTRYAG 1 cut(s) 81
BfoI RGCGCY 1 cut(s) 254
BfuAI ACCTGC 1 cut(s) 112
BglI GCCNNNNNGGC 1 cut(s) 96
BisI GCNGC 7 cut(s) 66, 88, 264, 267, 276, 392, 411
BlsI GCNGC 7 cut(s) 67, 89, 265, 268, 277, 393, 412
Bme1390I CCNGG 3 cut(s) 96, 239, 335
Bme18I GGWCC 2 cut(s) 125, 178
BmgT120I GGNCC 4 cut(s) 125, 178, 235, 580
BmiI GGNNCC 3 cut(s) 127, 223, 582
BmrFI CCNGG 3 cut(s) 96, 239, 335
BmsI GCATC 2 cut(s) 195, 297
BplI GAGNNNNNCTC 2 cut(s) 213, 245
Bpu10I CCTNAGC 1 cut(s) 254
BpuMI CCSGG 2 cut(s) 96, 335
BsaHI GRCGYC 2 cut(s) 381, 438
BsaJI CCNNGG 1 cut(s) 217
BsaWI WCCGGW 1 cut(s) 122
Bsc4I CCNNNNNNNGG 5 cut(s) 86, 110, 134, 135, 297
Bse1I ACTGG 1 cut(s) 328
Bse21I CCTNAGG 1 cut(s) 296
Bse3DI GCAATG 1 cut(s) 462
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 2 cut(s) 343, 403
BseLI CCNNNNNNNGG 5 cut(s) 86, 110, 134, 135, 297
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 2 cut(s) 268, 287
BseNI ACTGG 1 cut(s) 328
BseXI GCAGC 2 cut(s) 52, 403
Bsh1236I CGCG 2 cut(s) 269, 413
BshFI GGCC 7 cut(s) 90, 114, 237, 242, 263, 410, 581
BsiHKAI GWGCWC 1 cut(s) 48
BsiSI CCGG 3 cut(s) 95, 123, 335
BslFI GGGAC 1 cut(s) 111
BslI CCNNNNNNNGG 5 cut(s) 86, 110, 134, 135, 297
BsmFI GGGAC 1 cut(s) 111
BsmI GAATGC 1 cut(s) 277
BsnI GGCC 7 cut(s) 90, 114, 237, 242, 263, 410, 581
Bsp1286I GDGCHC 1 cut(s) 48
Bsp143I GATC 1 cut(s) 567
BspACI CCGC 5 cut(s) 87, 264, 267, 276, 411
BspANI GGCC 7 cut(s) 90, 114, 237, 242, 263, 410, 581
BspCNI CTCAG 2 cut(s) 267, 288
BspFNI CGCG 2 cut(s) 269, 413
BspLI GGNNCC 3 cut(s) 127, 223, 582
BspMI ACCTGC 1 cut(s) 112
BsrDI GCAATG 1 cut(s) 462
BsrI ACTGG 1 cut(s) 328
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 1 cut(s) 567
BssNI GRCGYC 2 cut(s) 381, 438
BssT1I CCWWGG 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 4 cut(s) 120, 371, 500, 511
BstACI GRCGYC 2 cut(s) 381, 438
BstC8I GCNNGC 4 cut(s) 44, 157, 261, 516
BstDEI CTNAG 2 cut(s) 254, 296
BstENI CCTNNNNNAGG 1 cut(s) 108
BstF5I GGATG 2 cut(s) 343, 403
BstFNI CGCG 2 cut(s) 269, 413
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 2 cut(s) 253, 415
BstKTI GATC 1 cut(s) 570
BstMBI GATC 1 cut(s) 567
BstMWI GCNNNNNNNGC 7 cut(s) 65, 96, 192, 248, 272, 275, 511
BstNI CCWGG 1 cut(s) 239
BstNSI RCATGY 2 cut(s) 56, 159
BstSCI CCNGG 3 cut(s) 94, 237, 333
BstSFI CTRYAG 1 cut(s) 81
BstUI CGCG 2 cut(s) 269, 413
BstV1I GCAGC 2 cut(s) 52, 403
Bsu36I CCTNAGG 1 cut(s) 296
BsuRI GGCC 7 cut(s) 90, 114, 237, 242, 263, 410, 581
BtsCI GGATG 2 cut(s) 343, 403
BtsIMutI CAGTG 2 cut(s) 139, 321
BveI ACCTGC 1 cut(s) 112
Cac8I GCNNGC 4 cut(s) 44, 157, 261, 516
CfoI GCGC 2 cut(s) 253, 415
Cfr13I GGNCC 4 cut(s) 125, 178, 235, 580
CseI GACGC 2 cut(s) 310, 389
CviAII CATG 2 cut(s) 53, 156
DdeI CTNAG 2 cut(s) 254, 296
DpnI GATC 1 cut(s) 569
DpnII GATC 1 cut(s) 567
DrdI GACNNNNNNGTC 1 cut(s) 443
DseDI GACNNNNNNGTC 1 cut(s) 443
EaeI YGGCCR 3 cut(s) 88, 261, 408
Eco130I CCWWGG 1 cut(s) 217
Eco47I GGWCC 2 cut(s) 125, 178
Eco47III AGCGCT 1 cut(s) 252
Eco57I CTGAAG 1 cut(s) 218
Eco81I CCTNAGG 1 cut(s) 296
EcoNI CCTNNNNNAGG 1 cut(s) 108
EcoO109I RGGNCCY 1 cut(s) 178
EcoRII CCWGG 1 cut(s) 237
EcoT14I CCWWGG 1 cut(s) 217
EcoT22I ATGCAT 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 2 cut(s) 56, 159
FaiI YATR 8 cut(s) 54, 157, 184, 248, 365, 375, 539, 595
FaqI GGGAC 1 cut(s) 111
FatI CATG 2 cut(s) 52, 155
FbaI TGATCA 1 cut(s) 567
Fnu4HI GCNGC 7 cut(s) 66, 88, 264, 267, 276, 392, 411
FokI GGATG 2 cut(s) 350, 410
Fsp4HI GCNGC 7 cut(s) 66, 88, 264, 267, 276, 392, 411
FspBI CTAG 3 cut(s) 146, 329, 494
GlaI GCGC 2 cut(s) 252, 414
GluI GCNGC 7 cut(s) 66, 88, 264, 267, 276, 392, 411
HaeII RGCGCY 1 cut(s) 254
HaeIII GGCC 7 cut(s) 90, 114, 237, 242, 263, 410, 581
HapII CCGG 3 cut(s) 95, 123, 335
HgaI GACGC 2 cut(s) 310, 389
HhaI GCGC 2 cut(s) 253, 415
Hin1I GRCGYC 2 cut(s) 381, 438
Hin1II CATG 2 cut(s) 56, 159
Hin6I GCGC 2 cut(s) 251, 413
HinP1I GCGC 2 cut(s) 251, 413
HinfI GANTC 4 cut(s) 139, 287, 401, 470
HpaII CCGG 3 cut(s) 95, 123, 335
HphI GGTGA 2 cut(s) 463, 512
Hpy188I TCNGA 1 cut(s) 421
Hpy188III TCNNGA 2 cut(s) 329, 356
Hpy99I CGWCG 4 cut(s) 172, 284, 383, 443
HpyAV CCTTC 2 cut(s) 253, 448
HpyCH4III ACNGT 4 cut(s) 120, 371, 500, 511
HpyCH4IV ACGT 2 cut(s) 282, 438
HpyCH4V TGCA 5 cut(s) 68, 159, 186, 363, 432
HpyF10VI GCNNNNNNNGC 7 cut(s) 65, 96, 192, 248, 272, 275, 511
HpyF3I CTNAG 2 cut(s) 254, 296
HpySE526I ACGT 2 cut(s) 282, 438
Hsp92I GRCGYC 2 cut(s) 381, 438
Hsp92II CATG 2 cut(s) 56, 159
HspAI GCGC 2 cut(s) 251, 413
Ksp22I TGATCA 1 cut(s) 567
Kzo9I GATC 1 cut(s) 567
LmnI GCTCC 3 cut(s) 51, 221, 494
Lsp1109I GCAGC 2 cut(s) 52, 403
LweI GCATC 2 cut(s) 195, 297
MaeI CTAG 3 cut(s) 146, 329, 494
MaeII ACGT 2 cut(s) 282, 438
MalI GATC 1 cut(s) 569
MboI GATC 1 cut(s) 567
MboII GAAGA 4 cut(s) 13, 16, 161, 211
MhlI GDGCHC 1 cut(s) 48
MluCI AATT 1 cut(s) 475
MlyI GAGTC 2 cut(s) 395, 464
MmeI TCCRAC 2 cut(s) 73, 399
MnlI CCTC 5 cut(s) 38, 71, 104, 239, 291
Mph1103I ATGCAT 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 394
MspI CCGG 3 cut(s) 95, 123, 335
MspR9I CCNGG 3 cut(s) 96, 239, 335
Mva1269I GAATGC 1 cut(s) 277
MvaI CCWGG 1 cut(s) 239
MvnI CGCG 2 cut(s) 269, 413
MwoI GCNNNNNNNGC 7 cut(s) 65, 96, 192, 248, 272, 275, 511
NciI CCSGG 2 cut(s) 96, 335
NdeII GATC 1 cut(s) 567
NlaIII CATG 2 cut(s) 56, 159
NlaIV GGNNCC 3 cut(s) 127, 223, 582
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 2 cut(s) 56, 159
PaeI GCATGC 1 cut(s) 159
PciI ACATGT 1 cut(s) 52
PctI GAATGC 1 cut(s) 277
PfeI GAWTC 2 cut(s) 139, 287
PkrI GCNGC 7 cut(s) 67, 89, 265, 268, 277, 393, 412
PleI GAGTC 2 cut(s) 395, 464
PpsI GAGTC 2 cut(s) 395, 464
PpuMI RGGWCCY 1 cut(s) 178
PscI ACATGT 1 cut(s) 52
Psp5II RGGWCCY 1 cut(s) 178
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 3 cut(s) 127, 223, 582
PspPI GGNCC 4 cut(s) 125, 178, 235, 580
PspPPI RGGWCCY 1 cut(s) 178
PvuII CAGCTG 1 cut(s) 394
SatI GCNGC 7 cut(s) 66, 88, 264, 267, 276, 392, 411
Sau3AI GATC 1 cut(s) 567
Sau96I GGNCC 4 cut(s) 125, 178, 235, 580
SchI GAGTC 2 cut(s) 395, 464
ScrFI CCNGG 3 cut(s) 96, 239, 335
SduI GDGCHC 1 cut(s) 48
SfaNI GCATC 2 cut(s) 195, 297
SfcI CTRYAG 1 cut(s) 81
SinI GGWCC 2 cut(s) 125, 178
SphI GCATGC 1 cut(s) 159
Sse9I AATT 1 cut(s) 475
SsiI CCGC 5 cut(s) 87, 264, 267, 276, 411
SspMI CTAG 3 cut(s) 146, 329, 494
StyD4I CCNGG 3 cut(s) 94, 237, 333
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 4 cut(s) 120, 371, 500, 511
TaiI ACGT 2 cut(s) 285, 441
TaqI TCGA 1 cut(s) 473
TasI AATT 1 cut(s) 475
TauI GCSGC 5 cut(s) 90, 266, 269, 278, 413
TfiI GAWTC 2 cut(s) 139, 287
TscAI CASTG 2 cut(s) 139, 328
TseI GCWGC 2 cut(s) 65, 391
TspDTI ATGAA 2 cut(s) 17, 235
TspGWI ACGGA 1 cut(s) 553
TspRI CASTG 2 cut(s) 139, 328
VpaK11BI GGWCC 2 cut(s) 125, 178
XagI CCTNNNNNAGG 1 cut(s) 108
XbaI TCTAGA 1 cut(s) 328
XceI RCATGY 2 cut(s) 56, 159
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XspI CTAG 3 cut(s) 146, 329, 494
ZraI GACGTC 1 cut(s) 439
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.