MD11G1307600.v1.1

Inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0015173
Physical Location & Seq
Reverse (-)
42179335 .. 42179928
594 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1307600.v1.1.491

Sequence Viewer

Length: 594 bp
ATGAGGCCAATTGCAGCAGCATTTTTCTTCCTCTCAACATTATGTGCCGCAAAATTTCTCGTCGTCGCTGGAAACGGTCAAGCTCAAGGTGTAAGTCAAGGCAGCAACACCGCCGCTGCCGCAGGTGAGGTGAACATGAAGCTTCTTGAACAAGCGTGCCAGCATTCACCCCGTAAGGACTTATGCATCGAAACCCTGGAGAAGGACCCGAACAGCAAGGGCGCGGACCTCACCGGCCTTGCCTTCGTTGCCATCCGGCTGGCTGCCGCAATGGCCTCCGACGTGGACGAGCACATGCGGTCGTTGCTCATTCACAACGCTACCACTTTGGACCCCACGATTCAGCAGGGGTTGGCAGACTGCATTGAGCACTACTCCGATGCCAACGAGCAGCTCGACGATTGCGTCGAGGCCATCTCGACGCATAACTTAGAGGAAGTGGAACTGTGGGTCAACGTGGCGATTTCCGACGCCGACTTATGCGACAGCTCGCTCGGGGGAGAGAAATCCGTGATCAAAGCCAAGAACGAAGCGTTCCGCCTCTTGTGCAATAATATTTTGTCCATCGTCAAGGTCTTGCCTGCTCACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

198

Amino Acids

21.05

Weight (kDa)

4.86

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 48 - 189 6.3e-21 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55770 AT4G02250
fragaria_vesca FvH4_2g25950
malus_domestica MD03G1290800.v1.1 MD11G1307600.v1.1
rosa_chinensis RchiOBHm_Chr6g0294271
rosa_laevigata RLG00000011882
rosa_multiflora Rmu_co8483625.1_g000001 Rmu_sc0003996.1_g000007
rosa_roxburghii Rroxscaffold_7G00173490
rosa_rugosa Rorug06G0243400
rosa_samantha Rh6AG355600 Rh6BG362500 Rh6CG369600 Rh6DG356400
rosa_wichuraiana Rw6G030980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 113
AasI GACNNNNNNGTC 1 cut(s) 404
Acc36I ACCTGC 1 cut(s) 113
AccII CGCG 1 cut(s) 224
AciI CCGC 8 cut(s) 48, 111, 114, 120, 224, 267, 298, 538
AcsI RAATTY 1 cut(s) 53
AcyI GRCGYC 1 cut(s) 471
AfiI CCNNNNNNNGG 1 cut(s) 202
AgsI TTSAA 1 cut(s) 149
AjiI CACGTC 1 cut(s) 283
AjnI CCWGG 1 cut(s) 195
AluBI AGCT 4 cut(s) 83, 142, 394, 489
AluI AGCT 4 cut(s) 83, 142, 394, 489
Alw21I GWGCWC 2 cut(s) 294, 372
Ama87I CYCGRG 1 cut(s) 494
AoxI GGCC 4 cut(s) 5, 235, 273, 411
ApeKI GCWGC 6 cut(s) 14, 17, 102, 116, 263, 391
ApoI RAATTY 1 cut(s) 53
AspLEI GCGC 1 cut(s) 224
AspS9I GGNCC 3 cut(s) 205, 226, 331
AsuHPI GGTGA 4 cut(s) 137, 142, 159, 223
AvaI CYCGRG 1 cut(s) 494
AvaII GGWCC 3 cut(s) 205, 226, 331
Bbv12I GWGCWC 2 cut(s) 294, 372
BbvI GCAGC 6 cut(s) 26, 29, 103, 114, 250, 403
BccI CCATC 3 cut(s) 260, 422, 572
BciT130I CCWGG 1 cut(s) 197
BclI TGATCA 1 cut(s) 513
BfuAI ACCTGC 1 cut(s) 113
BglI GCCNNNNNGGC 1 cut(s) 272
Bme1390I CCNGG 1 cut(s) 197
Bme18I GGWCC 3 cut(s) 205, 226, 331
BmeT110I CYCGRG 1 cut(s) 494
BmgBI CACGTC 1 cut(s) 283
BmgT120I GGNCC 3 cut(s) 205, 226, 331
BmiI GGNNCC 2 cut(s) 207, 333
BmrFI CCNGG 1 cut(s) 197
BmsI GCATC 2 cut(s) 195, 370
BplI GAGNNNNNCTC 4 cut(s) 359, 391, 401, 433
BpmI CTGGAG 1 cut(s) 218
BpuEI CTTGAG 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 471
BsaJI CCNNGG 1 cut(s) 195
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse118I RCCGGY 1 cut(s) 233
Bse3DI GCAATG 1 cut(s) 276
BseBI CCWGG 1 cut(s) 197
BseDI CCNNGG 1 cut(s) 195
BseGI GGATG 1 cut(s) 252
BseLI CCNNNNNNNGG 1 cut(s) 202
BseMI GCAATG 1 cut(s) 276
BseXI GCAGC 6 cut(s) 26, 29, 103, 114, 250, 403
Bsh1236I CGCG 1 cut(s) 224
Bsh1285I CGRYCG 1 cut(s) 302
BshFI GGCC 4 cut(s) 7, 237, 275, 413
BsiEI CGRYCG 1 cut(s) 302
BsiHKAI GWGCWC 2 cut(s) 294, 372
BsiHKCI CYCGRG 1 cut(s) 494
BsiSI CCGG 2 cut(s) 234, 256
BslI CCNNNNNNNGG 1 cut(s) 202
BsmI GAATGC 1 cut(s) 163
BsnI GGCC 4 cut(s) 7, 237, 275, 413
BsoBI CYCGRG 1 cut(s) 494
Bsp1286I GDGCHC 2 cut(s) 294, 372
Bsp143I GATC 1 cut(s) 513
BspACI CCGC 8 cut(s) 48, 111, 114, 120, 224, 267, 298, 538
BspANI GGCC 4 cut(s) 7, 237, 275, 413
BspFNI CGCG 1 cut(s) 224
BspLI GGNNCC 2 cut(s) 207, 333
BspMI ACCTGC 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 276
BsrFI RCCGGY 1 cut(s) 233
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 1 cut(s) 513
BssNI GRCGYC 1 cut(s) 471
Bst2UI CCWGG 1 cut(s) 197
Bst4CI ACNGT 2 cut(s) 77, 447
BstACI GRCGYC 1 cut(s) 471
BstC8I GCNNGC 5 cut(s) 157, 161, 261, 491, 582
BstDEI CTNAG 1 cut(s) 430
BstENI CCTNNNNNAGG 1 cut(s) 200
BstF5I GGATG 1 cut(s) 252
BstFNI CGCG 1 cut(s) 224
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 1 cut(s) 516
BstMBI GATC 1 cut(s) 513
BstMCI CGRYCG 1 cut(s) 302
BstMWI GCNNNNNNNGC 5 cut(s) 119, 248, 272, 304, 546
BstNI CCWGG 1 cut(s) 197
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 195
BstUI CGCG 1 cut(s) 224
BstV1I GCAGC 6 cut(s) 26, 29, 103, 114, 250, 403
BstXI CCANNNNNNTGG 1 cut(s) 259
BsuRI GGCC 4 cut(s) 7, 237, 275, 413
BtrI CACGTC 1 cut(s) 283
BtsCI GGATG 1 cut(s) 252
BveI ACCTGC 1 cut(s) 113
Cac8I GCNNGC 5 cut(s) 157, 161, 261, 491, 582
CfoI GCGC 1 cut(s) 224
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 3 cut(s) 205, 226, 331
CseI GACGC 3 cut(s) 394, 430, 479
CviAII CATG 2 cut(s) 136, 295
DdeI CTNAG 1 cut(s) 430
DpnI GATC 1 cut(s) 515
DpnII GATC 1 cut(s) 513
DrdI GACNNNNNNGTC 1 cut(s) 404
DseDI GACNNNNNNGTC 1 cut(s) 404
EciI GGCGGA 1 cut(s) 527
Eco47I GGWCC 3 cut(s) 205, 226, 331
Eco88I CYCGRG 1 cut(s) 494
EcoNI CCTNNNNNAGG 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 205
EcoRII CCWGG 1 cut(s) 195
EcoT22I ATGCAT 1 cut(s) 188
FaeI CATG 2 cut(s) 139, 298
FaiI YATR 6 cut(s) 43, 137, 184, 296, 426, 481
FatI CATG 2 cut(s) 135, 294
FbaI TGATCA 1 cut(s) 513
FokI GGATG 1 cut(s) 239
GlaI GCGC 1 cut(s) 223
GsuI CTGGAG 1 cut(s) 218
HaeIII GGCC 4 cut(s) 7, 237, 275, 413
HapII CCGG 2 cut(s) 234, 256
HgaI GACGC 3 cut(s) 394, 430, 479
HhaI GCGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 471
Hin1II CATG 2 cut(s) 139, 298
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 1 cut(s) 340
HpaII CCGG 2 cut(s) 234, 256
HphI GGTGA 4 cut(s) 137, 142, 159, 223
Hpy166II GTNNAC 3 cut(s) 133, 286, 454
Hpy188I TCNGA 3 cut(s) 280, 379, 469
Hpy188III TCNNGA 2 cut(s) 146, 418
Hpy8I GTNNAC 3 cut(s) 133, 286, 454
Hpy99I CGWCG 7 cut(s) 65, 68, 284, 401, 410, 424, 473
HpyAV CCTTC 2 cut(s) 196, 253
HpyCH4III ACNGT 2 cut(s) 77, 447
HpyCH4IV ACGT 2 cut(s) 282, 456
HpyCH4V TGCA 4 cut(s) 14, 186, 363, 549
HpyF10VI GCNNNNNNNGC 5 cut(s) 119, 248, 272, 304, 546
HpyF3I CTNAG 1 cut(s) 430
HpySE526I ACGT 2 cut(s) 282, 456
Hsp92I GRCGYC 1 cut(s) 471
Hsp92II CATG 2 cut(s) 139, 298
HspAI GCGC 1 cut(s) 222
Ksp22I TGATCA 1 cut(s) 513
Kzo9I GATC 1 cut(s) 513
LpnPI CCDG 9 cut(s) 54, 108, 173, 182, 209, 245, 247, 269, 332
Lsp1109I GCAGC 6 cut(s) 26, 29, 103, 114, 250, 403
LweI GCATC 2 cut(s) 195, 370
MaeII ACGT 2 cut(s) 282, 456
MalI GATC 1 cut(s) 515
MboI GATC 1 cut(s) 513
MboII GAAGA 1 cut(s) 19
MfeI CAATTG 1 cut(s) 9
MhlI GDGCHC 2 cut(s) 294, 372
MluCI AATT 2 cut(s) 9, 53
MmeI TCCRAC 2 cut(s) 303, 492
MnlI CCTC 7 cut(s) 41, 121, 239, 286, 403, 427, 551
Mph1103I ATGCAT 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 116
MspI CCGG 2 cut(s) 234, 256
MspR9I CCNGG 1 cut(s) 197
MunI CAATTG 1 cut(s) 9
Mva1269I GAATGC 1 cut(s) 163
MvaI CCWGG 1 cut(s) 197
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 5 cut(s) 119, 248, 272, 304, 546
NdeII GATC 1 cut(s) 513
NlaIII CATG 2 cut(s) 139, 298
NlaIV GGNNCC 2 cut(s) 207, 333
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 1 cut(s) 298
PaqCI CACCTGC 1 cut(s) 113
PcsI WCGNNNNNNNCGW 3 cut(s) 393, 402, 405
PctI GAATGC 1 cut(s) 163
PfeI GAWTC 1 cut(s) 340
PpuMI RGGWCCY 1 cut(s) 205
Psp5II RGGWCCY 1 cut(s) 205
Psp6I CCWGG 1 cut(s) 195
PspGI CCWGG 1 cut(s) 195
PspN4I GGNNCC 2 cut(s) 207, 333
PspPI GGNCC 3 cut(s) 205, 226, 331
PspPPI RGGWCCY 1 cut(s) 205
Sau3AI GATC 1 cut(s) 513
Sau96I GGNCC 3 cut(s) 205, 226, 331
ScrFI CCNGG 1 cut(s) 197
SduI GDGCHC 2 cut(s) 294, 372
SfaNI GCATC 2 cut(s) 195, 370
SinI GGWCC 3 cut(s) 205, 226, 331
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
Sse9I AATT 2 cut(s) 9, 53
SsiI CCGC 8 cut(s) 48, 111, 114, 120, 224, 267, 298, 538
SspI AATATT 1 cut(s) 556
StyD4I CCNGG 1 cut(s) 195
TaaI ACNGT 2 cut(s) 77, 447
TaiI ACGT 2 cut(s) 285, 459
TaqI TCGA 4 cut(s) 189, 396, 408, 419
TasI AATT 2 cut(s) 9, 53
TauI GCSGC 4 cut(s) 50, 116, 122, 269
TfiI GAWTC 1 cut(s) 340
TseI GCWGC 6 cut(s) 14, 17, 102, 116, 263, 391
TspDTI ATGAA 1 cut(s) 152
TspGWI ACGGA 1 cut(s) 499
VpaK11BI GGWCC 3 cut(s) 205, 226, 331
XagI CCTNNNNNAGG 1 cut(s) 200
XapI RAATTY 1 cut(s) 53
XceI RCATGY 1 cut(s) 298
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.