MD03G1290800.v1.1

Inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015173
Physical Location & Seq
Forward (+)
36828987 .. 36829580
594 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1290800.v1.1.491

Sequence Viewer

Length: 594 bp
ATGAAGCCAATTGCAGCAGCATTTTTCTTCCTCTCAACATTGTGTGCCGCAAGATTTGTCGTGGTCGCCGGTAATGGTCAAGGTCAAGGTGAAAGTGAAGGAAGCAACACCGCTGCCGCCGCGGGGGAGGTGAATATGAAGCTTCTTGAACAAGCGTGCCAGCATTCACCCAGGAAGGACTTATGCATCGAATCCCTAGAGAAGGACTCGAACAGCAAGGGCGCTGACCTCATAGGCCTCGCCTACATTGCCGTCCGGCTGGCCGCGGCAATGGCCGCCGACGTGGACGAGCACATGCGGTCGTTGCTCATGCACAACGCCACCACCTTGGACCCCATGATTCAGCAGGGGTTGGCGGACTGCGTTGAGCACTACTCCGATGCCAACGACCAGCTTGACGACTGCACCGCCGCCATCTCGGCGAGTAACTTCAAGGACGTTGAAGTGTGGGTCAACGTGGCGATTTCTGACGCCGACTTCTGCGACAAATCTCTTAAGGGACAGGAATCCGTGATGGTAGCGAAGAACAAAGCGTTCCGCCTGTTGTGCAATAATATTTTGTCCATAATCAAGGCCTTGCCTGCTGAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

198

Amino Acids

21.04

Weight (kDa)

4.84

Isoelectric Point (pI)

29.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 48 - 189 4.5e-22 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55770 AT4G02250
fragaria_vesca FvH4_2g25950
malus_domestica MD03G1290800.v1.1 MD11G1307600.v1.1
rosa_chinensis RchiOBHm_Chr6g0294271
rosa_laevigata RLG00000011882
rosa_multiflora Rmu_co8483625.1_g000001 Rmu_sc0003996.1_g000007
rosa_roxburghii Rroxscaffold_7G00173490
rosa_rugosa Rorug06G0243400
rosa_samantha Rh6AG355600 Rh6BG362500 Rh6CG369600 Rh6DG356400
rosa_wichuraiana Rw6G030980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 122, 266
AcoI YGGCCR 2 cut(s) 261, 273
AcyI GRCGYC 1 cut(s) 471
AfiI CCNNNNNNNGG 2 cut(s) 123, 202
AflII CTTAAG 1 cut(s) 494
AgsI TTSAA 3 cut(s) 149, 433, 443
AjiI CACGTC 1 cut(s) 283
AjnI CCWGG 1 cut(s) 170
AluBI AGCT 2 cut(s) 142, 394
AluI AGCT 2 cut(s) 142, 394
Alw21I GWGCWC 2 cut(s) 294, 372
AoxI GGCC 4 cut(s) 235, 261, 273, 573
ApeKI GCWGC 3 cut(s) 14, 17, 113
AspLEI GCGC 1 cut(s) 224
AspS9I GGNCC 1 cut(s) 331
AsuHPI GGTGA 3 cut(s) 101, 142, 159
AvaII GGWCC 1 cut(s) 331
Bbv12I GWGCWC 2 cut(s) 294, 372
BbvI GCAGC 3 cut(s) 26, 29, 100
BccI CCATC 2 cut(s) 422, 508
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 1 cut(s) 172
BfaI CTAG 1 cut(s) 197
BfoI RGCGCY 1 cut(s) 225
BfrI CTTAAG 1 cut(s) 494
BglI GCCNNNNNGGC 1 cut(s) 419
Bme1390I CCNGG 1 cut(s) 172
Bme18I GGWCC 1 cut(s) 331
BmgBI CACGTC 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 331
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 1 cut(s) 172
BmsI GCATC 2 cut(s) 195, 370
BplI GAGNNNNNCTC 4 cut(s) 191, 223, 359, 391
BsaHI GRCGYC 1 cut(s) 471
BsaJI CCNNGG 4 cut(s) 120, 170, 264, 327
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 202
Bse118I RCCGGY 1 cut(s) 68
Bse3DI GCAATG 2 cut(s) 246, 276
BseBI CCWGG 1 cut(s) 172
BseDI CCNNGG 4 cut(s) 120, 170, 264, 327
BseLI CCNNNNNNNGG 2 cut(s) 123, 202
BseMI GCAATG 2 cut(s) 246, 276
BseXI GCAGC 3 cut(s) 26, 29, 100
BsgI GTGCAG 1 cut(s) 388
Bsh1236I CGCG 2 cut(s) 122, 266
Bsh1285I CGRYCG 1 cut(s) 302
BshFI GGCC 4 cut(s) 237, 263, 275, 575
BsiEI CGRYCG 1 cut(s) 302
BsiHKAI GWGCWC 2 cut(s) 294, 372
BsiSI CCGG 2 cut(s) 69, 256
BslFI GGGAC 1 cut(s) 513
BslI CCNNNNNNNGG 2 cut(s) 123, 202
BsmFI GGGAC 1 cut(s) 513
BsmI GAATGC 1 cut(s) 163
BsnI GGCC 4 cut(s) 237, 263, 275, 575
Bsp1286I GDGCHC 2 cut(s) 294, 372
BspANI GGCC 4 cut(s) 237, 263, 275, 575
BspFNI CGCG 2 cut(s) 122, 266
BspLI GGNNCC 1 cut(s) 333
BspTI CTTAAG 1 cut(s) 494
BsrDI GCAATG 2 cut(s) 246, 276
BsrFI RCCGGY 1 cut(s) 68
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 4 cut(s) 120, 170, 264, 327
BssNI GRCGYC 1 cut(s) 471
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 1 cut(s) 172
BstACI GRCGYC 1 cut(s) 471
BstAFI CTTAAG 1 cut(s) 494
BstC8I GCNNGC 4 cut(s) 157, 161, 261, 582
BstDSI CCRYGG 2 cut(s) 120, 264
BstENI CCTNNNNNAGG 1 cut(s) 200
BstFNI CGCG 2 cut(s) 122, 266
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 1 cut(s) 224
BstMCI CGRYCG 1 cut(s) 302
BstMWI GCNNNNNNNGC 8 cut(s) 119, 248, 272, 275, 304, 419, 546, 581
BstNI CCWGG 1 cut(s) 172
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 170
BstUI CGCG 2 cut(s) 122, 266
BstV1I GCAGC 3 cut(s) 26, 29, 100
BstXI CCANNNNNNTGG 1 cut(s) 328
BsuRI GGCC 4 cut(s) 237, 263, 275, 575
BtgI CCRYGG 2 cut(s) 120, 264
BtrI CACGTC 1 cut(s) 283
Cac8I GCNNGC 4 cut(s) 157, 161, 261, 582
CfoI GCGC 1 cut(s) 224
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 331
Cfr42I CCGCGG 2 cut(s) 123, 267
CseI GACGC 1 cut(s) 479
CviAII CATG 3 cut(s) 295, 310, 337
CviJI RGCY 8 cut(s) 7, 142, 237, 259, 263, 275, 394, 575
CviKI_1 RGCY 8 cut(s) 7, 142, 237, 259, 263, 275, 394, 575
EaeI YGGCCR 2 cut(s) 261, 273
EciI GGCGGA 2 cut(s) 371, 527
Eco130I CCWWGG 1 cut(s) 327
Eco147I AGGCCT 2 cut(s) 237, 575
Eco47I GGWCC 1 cut(s) 331
EcoNI CCTNNNNNAGG 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 170
EcoT14I CCWWGG 1 cut(s) 327
EcoT22I ATGCAT 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 3 cut(s) 298, 313, 340
FaiI YATR 7 cut(s) 137, 184, 233, 296, 311, 338, 566
FaqI GGGAC 1 cut(s) 513
FatI CATG 3 cut(s) 294, 309, 336
FauI CCCGC 1 cut(s) 115
FspBI CTAG 1 cut(s) 197
GlaI GCGC 1 cut(s) 223
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 4 cut(s) 237, 263, 275, 575
HapII CCGG 2 cut(s) 69, 256
HgaI GACGC 1 cut(s) 479
HhaI GCGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 471
Hin1II CATG 3 cut(s) 298, 313, 340
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 4 cut(s) 191, 206, 340, 506
HpaII CCGG 2 cut(s) 69, 256
HphI GGTGA 3 cut(s) 101, 142, 159
Hpy166II GTNNAC 2 cut(s) 286, 454
Hpy188I TCNGA 2 cut(s) 379, 469
Hpy188III TCNNGA 1 cut(s) 146
Hpy8I GTNNAC 2 cut(s) 286, 454
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 3 cut(s) 92, 169, 196
HpyCH4IV ACGT 3 cut(s) 282, 438, 456
HpyCH4V TGCA 5 cut(s) 14, 186, 313, 405, 549
HpyF10VI GCNNNNNNNGC 8 cut(s) 119, 248, 272, 275, 304, 419, 546, 581
HpySE526I ACGT 3 cut(s) 282, 438, 456
Hsp92I GRCGYC 1 cut(s) 471
Hsp92II CATG 3 cut(s) 298, 313, 340
HspAI GCGC 1 cut(s) 222
KspI CCGCGG 2 cut(s) 123, 267
Lsp1109I GCAGC 3 cut(s) 26, 29, 100
LweI GCATC 2 cut(s) 195, 370
MaeI CTAG 1 cut(s) 197
MaeII ACGT 3 cut(s) 282, 438, 456
MaeIII GTNAC 1 cut(s) 425
MboII GAAGA 2 cut(s) 19, 535
MfeI CAATTG 1 cut(s) 9
MhlI GDGCHC 2 cut(s) 294, 372
MluCI AATT 1 cut(s) 9
MlyI GAGTC 1 cut(s) 200
MnlI CCTC 4 cut(s) 41, 121, 239, 248
Mph1103I ATGCAT 1 cut(s) 188
MseI TTAA 1 cut(s) 495
MspA1I CMGCKG 3 cut(s) 113, 122, 266
MspCI CTTAAG 1 cut(s) 494
MspI CCGG 2 cut(s) 69, 256
MspR9I CCNGG 1 cut(s) 172
MunI CAATTG 1 cut(s) 9
Mva1269I GAATGC 1 cut(s) 163
MvaI CCWGG 1 cut(s) 172
MvnI CGCG 2 cut(s) 122, 266
MwoI GCNNNNNNNGC 8 cut(s) 119, 248, 272, 275, 304, 419, 546, 581
NlaIII CATG 3 cut(s) 298, 313, 340
NlaIV GGNNCC 1 cut(s) 333
NmeAIII GCCGAG 1 cut(s) 398
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 1 cut(s) 298
PceI AGGCCT 2 cut(s) 237, 575
PctI GAATGC 1 cut(s) 163
PfeI GAWTC 3 cut(s) 191, 340, 506
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 170
PspGI CCWGG 1 cut(s) 170
PspN4I GGNNCC 1 cut(s) 333
PspPI GGNCC 1 cut(s) 331
SacII CCGCGG 2 cut(s) 123, 267
SaqAI TTAA 1 cut(s) 495
Sau96I GGNCC 1 cut(s) 331
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 1 cut(s) 172
SduI GDGCHC 2 cut(s) 294, 372
SfaNI GCATC 2 cut(s) 195, 370
Sfr303I CCGCGG 2 cut(s) 123, 267
SgrBI CCGCGG 2 cut(s) 123, 267
SinI GGWCC 1 cut(s) 331
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
Sse9I AATT 1 cut(s) 9
SseBI AGGCCT 2 cut(s) 237, 575
SspI AATATT 1 cut(s) 556
SspMI CTAG 1 cut(s) 197
StuI AGGCCT 2 cut(s) 237, 575
StyD4I CCNGG 1 cut(s) 170
StyI CCWWGG 1 cut(s) 327
TaiI ACGT 3 cut(s) 285, 441, 459
TaqI TCGA 2 cut(s) 189, 209
TasI AATT 1 cut(s) 9
TauI GCSGC 7 cut(s) 50, 119, 122, 266, 269, 278, 413
TfiI GAWTC 3 cut(s) 191, 340, 506
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TseI GCWGC 3 cut(s) 14, 17, 113
TspDTI ATGAA 2 cut(s) 17, 152
TspGWI ACGGA 1 cut(s) 499
Vha464I CTTAAG 1 cut(s) 494
VpaK11BI GGWCC 1 cut(s) 331
XagI CCTNNNNNAGG 1 cut(s) 200
XceI RCATGY 1 cut(s) 298
XspI CTAG 1 cut(s) 197
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.