Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007021

Physical Location & Seq

Genomic Coordinates Forward (+)

2218042 .. 2221260

Gene Structure 3219 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G04450.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1587 bp

ATGTTTCGTTTTCCGGTAAGTCTTGGAGGAGGTCCACGTGAGAATCTGAAGCCATCAGATGAGCAGCATCAACGTGCGGTGGTGAATGAGGTTGACTTTTTCCGATCAGCGGAGAAGAGAGATAGGGTTTCACGTGAAGAACAAAACATTATCGCCGATGAGACTCATAGGGTTCATGTCAAAAGGGAGAATTCACGTGTTGATGATCATGACGATCGTTCTACTGATCACATCAATATTGGACTTAATCTTCTCACTGCGAATACGGGAAGCGACGAGTCAATGGTGGATGATGGATTGTCTGTGGATATGGAAGAGAAACGTACAAAGTGTGAGAATGCACAACTTCGCGAAGAGCTAAAGAAGGCGAGTGAAGATAATCAAAGACTAAAGCAAATGCTAAGTCAAACAACCAACAACTTCAATTCCTTGCAGATGCAACTTGTTGCTGTCATGAGGCAACAAGAAGATCATCATCACCTAGCTACGACCGAGAACAATGACAATGTAAAGAACCGACATGAAGTGCCTGAAATGGTTCCAAGACAGTTCATCGATTTGGGACCGCATTCTGACGAAGTGTCGTCCGAGGAGAGGACGACGGTTCGGTCGGGATCTCCTCCCTCGCTTCTAGAGAAATCTAGCTCACGTCAAAACGGAAAGAGAGTGCTTGTAAGAGAAGAAAGCCCGGAAACCGAATCCAACGGCTGGAGAAACCCTAACAAAGTTCCTAAACACCATGCATCATCCAGCATTTGCGGTGGCAATGGCAGTGAAAATGCAAGTAGCAAGGTCATTGAGCAAGCGGCCGCCGAAGCCACCATGCGTAAAGCCCGTGTCTCGGTTCGTGCTCGATCCGAAGCTCCCATGTTAAGCGATGGATGTCAATGGAGAAAATACGGACAAAAAATGGCGAAAGGAAACCCGTGCCCTCGAGCTTATTACCGTTGCACAATGGCTGTTGGATGTCCTGTTCGCAAGCAAGTGCAACGTTGCGCGGAAGATAGAACCATTCTCATAACAACCTACGAAGGAAACCATAACCATCCATTACCTCCTGCGGCTATGAACATGGCTTCAACTACAACAGCAGCCGCAAGCATGCTTCTCTCAGGCTCCACCATGTCGAACCAAGACGGTTTAATGAACCCAACAAATCTCTTGGCTCGAACCATATTACCGTGTTCCTCAAGCATGGCTACTATCTCAGCCTCTGCACCATTCCCAACCATTACATTAGACCTCACAGAGTCACCCAACGGGAACAATCCAACCAATAACCCGCTGATGCAATTCTCTCAACGGTCTGGTTTGGTGGAGTTGAACCAATCGGTTTTGCCTCATATGATGGGTCAGGCTTTGTACTACAACCAACAGTCTAAGTTTTCGGGTTTACATATGCCGTCTCAGCCGCTAAACGCTGGTGAGAGTGTTAGCGCCGCTACTGCCGCAATCGCCTCCAATCCCAACTTTGCCGCGGCTCTAGCTGCAGCCATAACTTCGATTATCAACGGTTCGAACAATCAGCAGAATGGGAACAACAATAACAGTAATGTTACAACGAGCAACGTTGACAATAGGCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010033 GO:0010036 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016036 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042221 GO:0042493 GO:0042594 GO:0044212 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071496 GO:0080029 GO:0080090 GO:0080169 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

528

Amino Acids

58.07

Weight (kDa)

6.61

Isoelectric Point (pI)

48.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	293 - 350	1.8e-25	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007021)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62300 AT4G04450 AT4G04450 AT4G22070 AT4G22070 AT4G22070 AT4G22070
fragaria_vesca	FvH4_3g01700 FvH4_3g01700
malus_domestica	MD05G1349800.v1.1 MD10G1324500.v1.1
prunus_persica	Prupe.4G017600_v2.0.a1 Prupe.4G017600_v2.0.a1
pyrus_communis	pycom05g31740 pycom10g27490
rosa_chinensis	RchiOBHm_Chr5g0002561
rosa_laevigata	RLG00000031039
rosa_multiflora	Rmu_sc0010731.1_g000002
rosa_roxburghii	Rroxscaffold_1G00073350
rosa_rugosa	Rorug04G0396900
rosa_samantha	Rh5AG020300 Rh5BG023000 Rh5CG022300 Rh5DG021400
rosa_wichuraiana	Rw5G001870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	607
AccB7I	CCANNNNNTGG	1 cut(s)	708
AccII	CGCG	3 cut(s)	351, 998, 1478
AclI	AACGTT	2 cut(s)	991, 1569
AclWI	GGATC	2 cut(s)	622, 849
AcoI	YGGCCR	1 cut(s)	807
AcsI	RAATTY	1 cut(s)	190
AcuI	CTGAAG	1 cut(s)	68
AcvI	CACGTG	3 cut(s)	38, 134, 197
AfaI	GTAC	2 cut(s)	325, 1364
AfiI	CCNNNNNNNGG	4 cut(s)	109, 594, 708, 841
AflIII	ACRYGT	1 cut(s)	196
AgsI	TTSAA	3 cut(s)	424, 1080, 1324
AhdI	GACNNNNNGTC	1 cut(s)	580
AjiI	CACGTC	1 cut(s)	650
AloI	GAACNNNNNNTCC	2 cut(s)	957, 989
AluBI	AGCT	6 cut(s)	358, 485, 645, 863, 938, 1487
AluI	AGCT	6 cut(s)	358, 485, 645, 863, 938, 1487
Alw21I	GWGCWC	1 cut(s)	853
Alw26I	GTCTC	3 cut(s)	155, 844, 1410
AlwI	GGATC	2 cut(s)	622, 849
AlwNI	CAGNNNCTG	1 cut(s)	1214
Ama87I	CYCGRG	1 cut(s)	933
AoxI	GGCC	1 cut(s)	807
ApeKI	GCWGC	4 cut(s)	64, 1091, 1487, 1490
ApoI	RAATTY	1 cut(s)	190
ArsI	GACNNNNNNTTYG	2 cut(s)	388, 420
Asp700I	GAANNNNTTC	1 cut(s)	537
AspLEI	GCGC	2 cut(s)	998, 1439
AspS9I	GGNCC	2 cut(s)	32, 563
AsuC2I	CCSGG	1 cut(s)	689
AsuHPI	GGTGA	4 cut(s)	94, 470, 1245, 1436
AsuII	TTCGAA	1 cut(s)	1517
AvaI	CYCGRG	1 cut(s)	933
AvaII	GGWCC	2 cut(s)	32, 563
BaeGI	GKGCMC	1 cut(s)	932
BbrPI	CACGTG	3 cut(s)	38, 134, 197
Bbv12I	GWGCWC	1 cut(s)	853
BbvI	GCAGC	4 cut(s)	76, 1103, 1474, 1502
BccI	CCATC	5 cut(s)	61, 287, 872, 1053, 1342
BceAI	ACGGC	2 cut(s)	721, 1387
BclI	TGATCA	2 cut(s)	205, 226
BcnI	CCSGG	1 cut(s)	689
BcoDI	GTCTC	3 cut(s)	155, 844, 1410
BfaI	CTAG	4 cut(s)	482, 632, 642, 1484
BfmI	CTRYAG	1 cut(s)	1488
BfoI	RGCGCY	1 cut(s)	1440
Bme1390I	CCNGG	1 cut(s)	689
Bme18I	GGWCC	2 cut(s)	32, 563
BmeRI	GACNNNNNGTC	1 cut(s)	580
BmeT110I	CYCGRG	1 cut(s)	933
BmgBI	CACGTC	1 cut(s)	650
BmgT120I	GGNCC	2 cut(s)	32, 563
BmiI	GGNNCC	3 cut(s)	540, 564, 1117
BmrFI	CCNGG	1 cut(s)	689
BmsI	GCATC	4 cut(s)	76, 426, 752, 1278
BpmI	CTGGAG	1 cut(s)	730
Bpu14I	TTCGAA	1 cut(s)	1517
BpuEI	CTTGAG	1 cut(s)	1174
BpuMI	CCSGG	1 cut(s)	689
Bsa29I	ATCGAT	1 cut(s)	555
BsaAI	YACGTR	3 cut(s)	38, 134, 197
BsaBI	GATNNNNATC	1 cut(s)	474
BsaJI	CCNNGG	2 cut(s)	588, 1476
BsaWI	WCCGGW	1 cut(s)	13
Bsc4I	CCNNNNNNNGG	4 cut(s)	109, 594, 708, 841
Bse3DI	GCAATG	1 cut(s)	772
Bse8I	GATNNNNATC	1 cut(s)	474
BseCI	ATCGAT	1 cut(s)	555
BseDI	CCNNGG	2 cut(s)	588, 1476
BseGI	GGATG	5 cut(s)	295, 746, 887, 971, 1045
BseJI	GATNNNNATC	1 cut(s)	474
BseLI	CCNNNNNNNGG	4 cut(s)	109, 594, 708, 841
BseMI	GCAATG	1 cut(s)	772
BseMII	CTCAG	3 cut(s)	1125, 1221, 1421
BseRI	GAGGAG	3 cut(s)	42, 605, 609
BseSI	GKGCMC	1 cut(s)	932
BseX3I	CGGCCG	1 cut(s)	807
BseXI	GCAGC	4 cut(s)	76, 1103, 1474, 1502
BsgI	GTGCAG	1 cut(s)	1200
Bsh1236I	CGCG	3 cut(s)	351, 998, 1478
Bsh1285I	CGRYCG	4 cut(s)	217, 492, 611, 810
BshFI	GGCC	1 cut(s)	809
BshVI	ATCGAT	1 cut(s)	555
BsiEI	CGRYCG	4 cut(s)	217, 492, 611, 810
BsiHKAI	GWGCWC	1 cut(s)	853
BsiHKCI	CYCGRG	1 cut(s)	933
BsiSI	CCGG	2 cut(s)	14, 689
BslFI	GGGAC	1 cut(s)	576
BslI	CCNNNNNNNGG	4 cut(s)	109, 594, 708, 841
BsmAI	GTCTC	3 cut(s)	155, 844, 1410
BsmBI	CGTCTC	1 cut(s)	1410
BsmFI	GGGAC	1 cut(s)	576
BsmI	GAATGC	2 cut(s)	343, 568
BsnI	GGCC	1 cut(s)	809
BsoBI	CYCGRG	1 cut(s)	933
Bsp119I	TTCGAA	1 cut(s)	1517
Bsp1286I	GDGCHC	2 cut(s)	853, 932
Bsp143I	GATC	7 cut(s)	104, 205, 214, 226, 469, 614, 854
Bsp68I	TCGCGA	1 cut(s)	351
BspANI	GGCC	1 cut(s)	809
BspCNI	CTCAG	3 cut(s)	1124, 1220, 1420
BspDI	ATCGAT	1 cut(s)	555
BspFNI	CGCG	3 cut(s)	351, 998, 1478
BspHI	TCATGA	2 cut(s)	208, 453
BspLI	GGNNCC	3 cut(s)	540, 564, 1117
BspMAI	CTGCAG	1 cut(s)	1492
BspPI	GGATC	2 cut(s)	622, 849
BspQI	GCTCTTC	1 cut(s)	348
BspT104I	TTCGAA	1 cut(s)	1517
BsrDI	GCAATG	1 cut(s)	772
BssECI	CCNNGG	2 cut(s)	588, 1476
BssMI	GATC	7 cut(s)	104, 205, 214, 226, 469, 614, 854
Bst4CI	ACNGT	9 cut(s)	549, 604, 947, 1139, 1182, 1305, 1377, 1514, 1550
Bst6I	CTCTTC	3 cut(s)	110, 309, 348
BstBAI	YACGTR	3 cut(s)	38, 134, 197
BstBI	TTCGAA	1 cut(s)	1517
BstC8I	GCNNGC	4 cut(s)	804, 980, 1099, 1103
BstDEI	CTNAG	5 cut(s)	401, 1111, 1207, 1380, 1407
BstDSI	CCRYGG	1 cut(s)	1476
BstF5I	GGATG	5 cut(s)	295, 746, 887, 971, 1045
BstFNI	CGCG	3 cut(s)	351, 998, 1478
BstH2I	RGCGCY	1 cut(s)	1440
BstHHI	GCGC	2 cut(s)	998, 1439
BstKTI	GATC	7 cut(s)	107, 208, 217, 229, 472, 617, 857
BstMAI	GTCTC	3 cut(s)	155, 844, 1410
BstMBI	GATC	7 cut(s)	104, 205, 214, 226, 469, 614, 854
BstMCI	CGRYCG	4 cut(s)	217, 492, 611, 810
BstMWI	GCNNNNNNNGC	7 cut(s)	815, 1408, 1445, 1448, 1454, 1484, 1487
BstNSI	RCATGY	1 cut(s)	1105
BstSCI	CCNGG	1 cut(s)	687
BstSFI	CTRYAG	1 cut(s)	1488
BstSLI	GKGCMC	1 cut(s)	932
BstUI	CGCG	3 cut(s)	351, 998, 1478
BstV1I	GCAGC	4 cut(s)	76, 1103, 1474, 1502
BstX2I	RGATCY	1 cut(s)	614
BstYI	RGATCY	1 cut(s)	614
BstZI	CGGCCG	1 cut(s)	807
Bsu15I	ATCGAT	1 cut(s)	555
BsuRI	GGCC	1 cut(s)	809
BsuTUI	ATCGAT	1 cut(s)	555
BtgI	CCRYGG	1 cut(s)	1476
BtgZI	GCGATG	1 cut(s)	891
BtrI	CACGTC	1 cut(s)	650
BtsCI	GGATG	5 cut(s)	295, 746, 887, 971, 1045
BtsI	GCAGTG	2 cut(s)	255, 778
BtsIMutI	CAGTG	2 cut(s)	255, 778
BtuMI	TCGCGA	1 cut(s)	351
Cac8I	GCNNGC	4 cut(s)	804, 980, 1099, 1103
CaiI	CAGNNNCTG	1 cut(s)	1214
CciI	TCATGA	2 cut(s)	208, 453
CciNI	GCGGCCGC	1 cut(s)	807
CfoI	GCGC	2 cut(s)	998, 1439
Cfr13I	GGNCC	2 cut(s)	32, 563
Cfr42I	CCGCGG	1 cut(s)	1479
ClaI	ATCGAT	1 cut(s)	555
Csp6I	GTAC	2 cut(s)	324, 1363
CviQI	GTAC	2 cut(s)	324, 1363
DdeI	CTNAG	5 cut(s)	401, 1111, 1207, 1380, 1407
DpnI	GATC	7 cut(s)	106, 207, 216, 228, 471, 616, 856
DpnII	GATC	7 cut(s)	104, 205, 214, 226, 469, 614, 854
DrdI	GACNNNNNNGTC	1 cut(s)	607
DriI	GACNNNNNGTC	1 cut(s)	580
DseDI	GACNNNNNNGTC	1 cut(s)	607
EaeI	YGGCCR	1 cut(s)	807
EagI	CGGCCG	1 cut(s)	807
Eam1104I	CTCTTC	3 cut(s)	110, 309, 348
Eam1105I	GACNNNNNGTC	1 cut(s)	580
EarI	CTCTTC	3 cut(s)	110, 309, 348
EclXI	CGGCCG	1 cut(s)	807
Eco47I	GGWCC	2 cut(s)	32, 563
Eco52I	CGGCCG	1 cut(s)	807
Eco57I	CTGAAG	1 cut(s)	68
Eco72I	CACGTG	3 cut(s)	38, 134, 197
Eco88I	CYCGRG	1 cut(s)	933
EcoRI	GAATTC	1 cut(s)	190
EcoT22I	ATGCAT	1 cut(s)	745
Esp3I	CGTCTC	1 cut(s)	1410
FaqI	GGGAC	1 cut(s)	576
FauI	CCCGC	1 cut(s)	1290
FauNDI	CATATG	2 cut(s)	1344, 1398
FbaI	TGATCA	2 cut(s)	205, 226
FokI	GGATG	5 cut(s)	302, 733, 894, 978, 1032
FspBI	CTAG	4 cut(s)	482, 632, 642, 1484
GlaI	GCGC	2 cut(s)	997, 1438
GsuI	CTGGAG	1 cut(s)	730
HaeII	RGCGCY	1 cut(s)	1440
HaeIII	GGCC	1 cut(s)	809
HapII	CCGG	2 cut(s)	14, 689
HhaI	GCGC	2 cut(s)	998, 1439
Hin6I	GCGC	2 cut(s)	996, 1437
HinP1I	GCGC	2 cut(s)	996, 1437
HincII	GTYRAC	2 cut(s)	94, 1573
HindII	GTYRAC	2 cut(s)	94, 1573
HinfI	GANTC	5 cut(s)	43, 163, 278, 698, 1250
HpaII	CCGG	2 cut(s)	14, 689
HphI	GGTGA	4 cut(s)	94, 470, 1245, 1436
Hpy166II	GTNNAC	4 cut(s)	35, 94, 1394, 1573
Hpy188I	TCNGA	6 cut(s)	48, 58, 104, 574, 589, 859
Hpy188III	TCNNGA	5 cut(s)	209, 350, 454, 612, 632
Hpy8I	GTNNAC	4 cut(s)	35, 94, 1394, 1573
Hpy99I	CGWCG	2 cut(s)	278, 604
HpyAV	CCTTC	2 cut(s)	358, 1025
HpyCH4III	ACNGT	9 cut(s)	549, 604, 947, 1139, 1182, 1305, 1377, 1514, 1550
HpyCH4IV	ACGT	8 cut(s)	37, 73, 133, 196, 322, 649, 991, 1569
HpyF10VI	GCNNNNNNNGC	7 cut(s)	815, 1408, 1445, 1448, 1454, 1484, 1487
HpyF3I	CTNAG	5 cut(s)	401, 1111, 1207, 1380, 1407
HpySE526I	ACGT	8 cut(s)	37, 73, 133, 196, 322, 649, 991, 1569
HspAI	GCGC	2 cut(s)	996, 1437
Ksp22I	TGATCA	2 cut(s)	205, 226
KspI	CCGCGG	1 cut(s)	1479
Kzo9I	GATC	7 cut(s)	104, 205, 214, 226, 469, 614, 854
LguI	GCTCTTC	1 cut(s)	348
LmnI	GCTCC	2 cut(s)	868, 1121
Lsp1109I	GCAGC	4 cut(s)	76, 1103, 1474, 1502
LweI	GCATC	4 cut(s)	76, 426, 752, 1278
MaeI	CTAG	4 cut(s)	482, 632, 642, 1484
MaeII	ACGT	8 cut(s)	37, 73, 133, 196, 322, 649, 991, 1569
MaeIII	GTNAC	2 cut(s)	1251, 1555
MalI	GATC	7 cut(s)	106, 207, 216, 228, 471, 616, 856
MboI	GATC	7 cut(s)	104, 205, 214, 226, 469, 614, 854
MboII	GAAGA	9 cut(s)	127, 149, 242, 326, 365, 386, 479, 692, 1013
MflI	RGATCY	1 cut(s)	614
MhlI	GDGCHC	2 cut(s)	853, 932
MluCI	AATT	3 cut(s)	190, 424, 1292
MlyI	GAGTC	3 cut(s)	157, 287, 1259
MmeI	TCCRAC	3 cut(s)	726, 943, 1295
Mph1103I	ATGCAT	1 cut(s)	745
MroXI	GAANNNNTTC	1 cut(s)	537
MseI	TTAA	3 cut(s)	246, 872, 1142
MslI	CAYNNNNRTG	1 cut(s)	72
MspA1I	CMGCKG	3 cut(s)	110, 1285, 1478
MspI	CCGG	2 cut(s)	14, 689
MspR9I	CCNGG	1 cut(s)	689
Mva1269I	GAATGC	2 cut(s)	343, 568
MvnI	CGCG	3 cut(s)	351, 998, 1478
MwoI	GCNNNNNNNGC	7 cut(s)	815, 1408, 1445, 1448, 1454, 1484, 1487
NciI	CCSGG	1 cut(s)	689
NdeI	CATATG	2 cut(s)	1344, 1398
NdeII	GATC	7 cut(s)	104, 205, 214, 226, 469, 614, 854
NlaIV	GGNNCC	3 cut(s)	540, 564, 1117
NmuCI	GTSAC	1 cut(s)	1251
NotI	GCGGCCGC	1 cut(s)	807
NruI	TCGCGA	1 cut(s)	351
NsiI	ATGCAT	1 cut(s)	745
NspI	RCATGY	1 cut(s)	1105
NspV	TTCGAA	1 cut(s)	1517
PaeI	GCATGC	1 cut(s)	1105
PaeR7I	CTCGAG	1 cut(s)	933
PagI	TCATGA	2 cut(s)	208, 453
PciSI	GCTCTTC	1 cut(s)	348
PctI	GAATGC	2 cut(s)	343, 568
PdmI	GAANNNNTTC	1 cut(s)	537
PfeI	GAWTC	2 cut(s)	43, 698
PflMI	CCANNNNNTGG	1 cut(s)	708
Ple19I	CGATCG	1 cut(s)	217
PleI	GAGTC	3 cut(s)	157, 286, 1258
PmaCI	CACGTG	3 cut(s)	38, 134, 197
PmlI	CACGTG	3 cut(s)	38, 134, 197
PpsI	GAGTC	3 cut(s)	157, 286, 1258
Ppu21I	YACGTR	3 cut(s)	38, 134, 197
Psp1406I	AACGTT	2 cut(s)	991, 1569
PspCI	CACGTG	3 cut(s)	38, 134, 197
PspN4I	GGNNCC	3 cut(s)	540, 564, 1117
PspPI	GGNCC	2 cut(s)	32, 563
PspXI	VCTCGAGB	1 cut(s)	933
PstI	CTGCAG	1 cut(s)	1492
PstNI	CAGNNNCTG	1 cut(s)	1214
PsuI	RGATCY	1 cut(s)	614
PvuI	CGATCG	1 cut(s)	217
RruI	TCGCGA	1 cut(s)	351
RsaI	GTAC	2 cut(s)	325, 1364
RsaNI	GTAC	2 cut(s)	324, 1363
RseI	CAYNNNNRTG	1 cut(s)	72
SacII	CCGCGG	1 cut(s)	1479
SapI	GCTCTTC	1 cut(s)	348
SaqAI	TTAA	3 cut(s)	246, 872, 1142
Sau3AI	GATC	7 cut(s)	104, 205, 214, 226, 469, 614, 854
Sau96I	GGNCC	2 cut(s)	32, 563
SchI	GAGTC	3 cut(s)	157, 287, 1259
ScrFI	CCNGG	1 cut(s)	689
SduI	GDGCHC	2 cut(s)	853, 932
SfaNI	GCATC	4 cut(s)	76, 426, 752, 1278
SfcI	CTRYAG	1 cut(s)	1488
Sfr274I	CTCGAG	1 cut(s)	933
Sfr303I	CCGCGG	1 cut(s)	1479
SfuI	TTCGAA	1 cut(s)	1517
SgrBI	CCGCGG	1 cut(s)	1479
SinI	GGWCC	2 cut(s)	32, 563
SlaI	CTCGAG	1 cut(s)	933
SmiMI	CAYNNNNRTG	1 cut(s)	72
SmlI	CTYRAG	2 cut(s)	933, 1189
SmoI	CTYRAG	2 cut(s)	933, 1189
SphI	GCATGC	1 cut(s)	1105
Sse9I	AATT	3 cut(s)	190, 424, 1292
SspI	AATATT	1 cut(s)	238
SspMI	CTAG	4 cut(s)	482, 632, 642, 1484
StyD4I	CCNGG	1 cut(s)	687
TaaI	ACNGT	9 cut(s)	549, 604, 947, 1139, 1182, 1305, 1377, 1514, 1550
TaiI	ACGT	8 cut(s)	40, 76, 136, 199, 325, 652, 994, 1572
TaqI	TCGA	7 cut(s)	555, 853, 934, 1127, 1168, 1502, 1517
TaqII	GACCGA	2 cut(s)	506, 597
TasI	AATT	3 cut(s)	190, 424, 1292
TatI	WGTACW	1 cut(s)	1362
TauI	GCSGC	9 cut(s)	809, 812, 1064, 1097, 1414, 1442, 1451, 1478, 1481
TfiI	GAWTC	2 cut(s)	43, 698
Tru1I	TTAA	3 cut(s)	246, 872, 1142
Tru9I	TTAA	3 cut(s)	246, 872, 1142
TscAI	CASTG	2 cut(s)	262, 778
TseFI	GTSAC	1 cut(s)	1251
TseI	GCWGC	4 cut(s)	64, 1091, 1487, 1490
Tsp45I	GTSAC	1 cut(s)	1251
TspDTI	ATGAA	5 cut(s)	164, 537, 541, 1082, 1160
TspGWI	ACGGA	2 cut(s)	672, 915
TspRI	CASTG	2 cut(s)	262, 778
Van91I	CCANNNNNTGG	1 cut(s)	708
VpaK11BI	GGWCC	2 cut(s)	32, 563
XapI	RAATTY	1 cut(s)	190
XbaI	TCTAGA	1 cut(s)	631
XceI	RCATGY	1 cut(s)	1105
XhoI	CTCGAG	1 cut(s)	933
XmnI	GAANNNNTTC	1 cut(s)	537
XspI	CTAG	4 cut(s)	482, 632, 642, 1484
Zsp2I	ATGCAT	1 cut(s)	745

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.