Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0007021

Physical Location & Seq

Genomic Coordinates Forward (+)

1435211 .. 1438007

Gene Structure 2797 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG020300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1740 bp

ATGGACAAAGGATGGGGTCTAACCCTTGATTCCGAATCCCTCGGCTTCTTCCTCAACAAGCCTCCTCCGTCTGCTGATCCTCCTCCTAATAAGAGAACAAACCCGTTTGGCGGTGGTGATAGGATGTTTTCCGGTATTGAATTCCCAGTCAAGTCAAGGCCGGCCGATGAGAACCGCGTGGCCGTTGACGAGGTCGACTTCTTCTCTGACAAGAACAAGAACAAGAATGATGATCAAGAGTTGAAAGCCAGCAACAATACCAGTGTCAGTGTCAAGAAGGAGAACTGGTCTGGTTTGGACGCTGTAAACACTGTCTTGCACCTTGTTACTGTAAACACCGGAAGTGATCAATCGACGGTGGATGATGGGGTGTCATCTGATCAGGTGGATAATAAAAGAGCAAAGATTCATGAGCTGGCACAGTTGCAATTTGAGCTCCAGCGTATGAATGCTGAGAATCAGAGACTGAAGGAAATGCTTGGTCAAGTGACCGACAACTACAAGGCTTTACAGATGCATCTTGTGGCAGTAATGCAGCAACAGCAGCAAAGCCAAGCCGCAGCTGATCAAACCTCCCAGGATCATGATCAGAATATTGAAGGAAAGTCTGAGGAGAAGAGACATGAGATTGGATCATCAGGATCATTGGTGCCTAGGCAGTTTCTGAACTTGGGTCCAAGGGAAGAAACAAAGGCGGATAATGAGGTTTCGAATTCTTCATCCGAAGCCAGAACTCTATCAGCTTCACCTCAGAATAATGTTAGTACTAATGGCAAAAGGGTTGGTAGGGAAGAGAGTCCCGAGTCTGAAACACAAGGCTGCGAGCCTAACAAGGTAGCTAAGCACAACTCCCCACCTAAGCAGATTGATCAGGCCACCGAGGCCACCATGAGGAAAGCTCGAGTCTCAGTTCGAGCCAGATCGGAAGCTCCTATGATCACTGATGGATGCCAATGGCGAAAATATGGACAAAAGATGGCGAAAGGAAACCCATGTCCTCGTGCTTATTATCGCTGTACCATGGCCGTTGGCTGTCCAGTTCGTAAACAGGTACAACGTTGCGCAGAAGACAGGACCATTTTGATCACAACTTACGAAGGCAACCACAACCACCCGCTGCCTCCTGCGGCAATGGCCATGGCATCCACCACTACTGCAGCGGCTAGCATGTTACTTTCGGGGTCCATGTCAAGTGCAGACGGGATCATGAACCCGAACAATTTGCTAGCACGTGCCATTCTTCCATGCTCATCAAGTGTGGCAACAATCTCAGCCTCAGCTCCATTCCCTACCGTCACATTAGACCTCACCACCAGTCCACCATTACAGTTCCAAAGACCACAACAAGGCCAGTTCCAGCTGCCTTTCCCAGGACAGCCCCAGAACTTCGGACCATCAGCCTCAAACCCTCAACTGCCCCAAGGTTTTGGACAAGCCCTATACAACCAGTCTAAATTCTCTGGGCTTCAACTCTCGCAGGAACTAATGAACTCGACCTTAGCGCACCAAACACAACAACAACAACAACAACAAGGTCAACCATCCTCGTTTGCAGACACAGTGAGTGCTGCCACAGCTGCCATCACTGCTGATCCGACATTCACTGCCGCCCTAGCTGCCGCTATCACCTCCATTATCGGCGGATCGTCTCATCCCAACAACAACAACTCCACAACCTCAAACAACAGCAATGGTGGCAACAATAACAACAACAAAATCAACAGCTTCCCGGGAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010033 GO:0010036 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016036 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042221 GO:0042493 GO:0042594 GO:0044212 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071496 GO:0080029 GO:0080090 GO:0080169 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

579

Amino Acids

62.57

Weight (kDa)

6.39

Isoelectric Point (pI)

50.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	315 - 372	2.4e-25	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007021)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62300 AT4G04450 AT4G04450 AT4G22070 AT4G22070 AT4G22070 AT4G22070
fragaria_vesca	FvH4_3g01700 FvH4_3g01700
malus_domestica	MD05G1349800.v1.1 MD10G1324500.v1.1
prunus_persica	Prupe.4G017600_v2.0.a1 Prupe.4G017600_v2.0.a1
pyrus_communis	pycom05g31740 pycom10g27490
rosa_chinensis	RchiOBHm_Chr5g0002561
rosa_laevigata	RLG00000031039
rosa_multiflora	Rmu_sc0010731.1_g000002
rosa_roxburghii	Rroxscaffold_1G00073350
rosa_rugosa	Rorug04G0396900
rosa_samantha	Rh5AG020300 Rh5BG023000 Rh5CG022300 Rh5DG021400
rosa_wichuraiana	Rw5G001870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1063
AccB1I	GGYRCC	1 cut(s)	649
AccI	GTMKAC	1 cut(s)	195
AccII	CGCG	1 cut(s)	177
AclI	AACGTT	1 cut(s)	1057
AclWI	GGATC	7 cut(s)	71, 588, 640, 649, 1211, 1586, 1651
AcoI	YGGCCR	4 cut(s)	162, 180, 1023, 1134
AcsI	RAATTY	3 cut(s)	140, 712, 1454
AcuI	CTGAAG	1 cut(s)	488
AcvI	CACGTG	1 cut(s)	1232
AfaI	GTAC	3 cut(s)	766, 1018, 1053
AfiI	CCNNNNNNNGG	4 cut(s)	110, 891, 1346, 1370
AgsI	TTSAA	4 cut(s)	140, 244, 599, 1469
AjnI	CCWGG	2 cut(s)	576, 1369
Alw21I	GWGCWC	1 cut(s)	438
Alw26I	GTCTC	4 cut(s)	457, 613, 910, 1653
AlwI	GGATC	7 cut(s)	71, 588, 640, 649, 1211, 1586, 1651
AlwNI	CAGNNNCTG	2 cut(s)	466, 664
Ama87I	CYCGRG	3 cut(s)	800, 900, 1729
AoxI	GGCC	8 cut(s)	158, 162, 180, 873, 882, 1023, 1134, 1348
ApoI	RAATTY	3 cut(s)	140, 712, 1454
AspA2I	CCTAGG	1 cut(s)	653
AspLEI	GCGC	2 cut(s)	1064, 1504
AspS9I	GGNCC	4 cut(s)	674, 1074, 1182, 1391
AsuC2I	CCSGG	2 cut(s)	1730, 1731
AsuHPI	GGTGA	4 cut(s)	128, 738, 1300, 1618
AsuII	TTCGAA	1 cut(s)	710
AsuNHI	GCTAGC	2 cut(s)	1163, 1225
AvaI	CYCGRG	3 cut(s)	800, 900, 1729
AvaII	GGWCC	4 cut(s)	674, 1074, 1182, 1391
AvrII	CCTAGG	1 cut(s)	653
BalI	TGGCCA	1 cut(s)	1136
BanI	GGYRCC	1 cut(s)	649
BanII	GRGCYC	1 cut(s)	438
BauI	CACGAG	1 cut(s)	999
BbrPI	CACGTG	1 cut(s)	1232
BbsI	GAAGAC	1 cut(s)	1074
Bbv12I	GWGCWC	1 cut(s)	438
BbvCI	CCTCAGC	1 cut(s)	1276
BccI	CCATC	7 cut(s)	6, 359, 938, 970, 1402, 1549, 1589
BceAI	ACGGC	2 cut(s)	167, 1010
BcgI	CGANNNNNNTGC	2 cut(s)	1204, 1238
BciT130I	CCWGG	2 cut(s)	578, 1371
BclI	TGATCA	8 cut(s)	232, 346, 379, 565, 586, 868, 936, 1083
BcnI	CCSGG	2 cut(s)	1730, 1731
BcoDI	GTCTC	4 cut(s)	457, 613, 910, 1653
BfaI	CTAG	4 cut(s)	654, 1164, 1226, 1613
BfmI	CTRYAG	1 cut(s)	1155
BglI	GCCNNNNNGGC	1 cut(s)	881
BlnI	CCTAGG	1 cut(s)	653
BlpI	GCTNAGC	1 cut(s)	840
BmcAI	AGTACT	1 cut(s)	766
Bme1390I	CCNGG	4 cut(s)	578, 1371, 1730, 1731
Bme18I	GGWCC	4 cut(s)	674, 1074, 1182, 1391
BmeT110I	CYCGRG	3 cut(s)	800, 900, 1729
BmgT120I	GGNCC	4 cut(s)	674, 1074, 1182, 1391
BmiI	GGNNCC	3 cut(s)	651, 675, 1183
BmrFI	CCNGG	4 cut(s)	578, 1371, 1730, 1731
BmrI	ACTGGG	1 cut(s)	140
BmsI	GCATC	4 cut(s)	504, 526, 938, 1151
BmtI	GCTAGC	2 cut(s)	1167, 1229
BmuI	ACTGGG	1 cut(s)	140
BpiI	GAAGAC	1 cut(s)	1074
BplI	GAGNNNNNCTC	2 cut(s)	883, 915
BpmI	CTGGAG	1 cut(s)	422
Bpu10I	CCTNAGC	3 cut(s)	858, 1276, 1498
Bpu1102I	GCTNAGC	1 cut(s)	840
Bpu14I	TTCGAA	1 cut(s)	710
BpuMI	CCSGG	2 cut(s)	1730, 1731
BsaAI	YACGTR	1 cut(s)	1232
BsaBI	GATNNNNATC	1 cut(s)	585
BsaWI	WCCGGW	2 cut(s)	131, 338
BsaXI	ACNNNNNCTCC	4 cut(s)	272, 302, 1652, 1682
Bsc4I	CCNNNNNNNGG	4 cut(s)	110, 891, 1346, 1370
Bse118I	RCCGGY	1 cut(s)	160
Bse1I	ACTGG	7 cut(s)	146, 261, 290, 1037, 1314, 1351, 1447
Bse3DI	GCAATG	2 cut(s)	1137, 1696
Bse8I	GATNNNNATC	1 cut(s)	585
BseBI	CCWGG	2 cut(s)	578, 1371
BseGI	GGATG	8 cut(s)	17, 129, 367, 719, 953, 1142, 1541, 1651
BseJI	GATNNNNATC	1 cut(s)	585
BseLI	CCNNNNNNNGG	4 cut(s)	110, 891, 1346, 1370
BseMI	GCAATG	2 cut(s)	1137, 1696
BseMII	CTCAG	6 cut(s)	444, 600, 764, 921, 1284, 1290
BseNI	ACTGG	7 cut(s)	146, 261, 290, 1037, 1314, 1351, 1447
BseRI	GAGGAG	3 cut(s)	54, 72, 626
BseX3I	CGGCCG	1 cut(s)	162
BsgI	GTGCAG	1 cut(s)	1215
Bsh1236I	CGCG	1 cut(s)	177
Bsh1285I	CGRYCG	1 cut(s)	165
BshFI	GGCC	8 cut(s)	160, 164, 182, 875, 884, 1025, 1136, 1350
BshNI	GGYRCC	1 cut(s)	649
BsiEI	CGRYCG	1 cut(s)	165
BsiHKAI	GWGCWC	1 cut(s)	438
BsiHKCI	CYCGRG	3 cut(s)	800, 900, 1729
BsiSI	CCGG	4 cut(s)	132, 161, 339, 1730
BslFI	GGGAC	1 cut(s)	783
BslI	CCNNNNNNNGG	4 cut(s)	110, 891, 1346, 1370
BsmAI	GTCTC	4 cut(s)	457, 613, 910, 1653
BsmBI	CGTCTC	1 cut(s)	1653
BsmFI	GGGAC	1 cut(s)	783
BsmI	GAATGC	1 cut(s)	454
BsnI	GGCC	8 cut(s)	160, 164, 182, 875, 884, 1025, 1136, 1350
BsoBI	CYCGRG	3 cut(s)	800, 900, 1729
Bsp119I	TTCGAA	1 cut(s)	710
Bsp1286I	GDGCHC	1 cut(s)	438
Bsp1720I	GCTNAGC	1 cut(s)	840
Bsp19I	CCATGG	2 cut(s)	1020, 1137
BspANI	GGCC	8 cut(s)	160, 164, 182, 875, 884, 1025, 1136, 1350
BspCNI	CTCAG	6 cut(s)	445, 601, 763, 920, 1283, 1289
BspFNI	CGCG	1 cut(s)	177
BspHI	TCATGA	3 cut(s)	409, 583, 1206
BspLI	GGNNCC	3 cut(s)	651, 675, 1183
BspMAI	CTGCAG	1 cut(s)	1159
BspOI	GCTAGC	2 cut(s)	1167, 1229
BspPI	GGATC	7 cut(s)	71, 588, 640, 649, 1211, 1586, 1651
BspT104I	TTCGAA	1 cut(s)	710
BspT107I	GGYRCC	1 cut(s)	649
BsrDI	GCAATG	2 cut(s)	1137, 1696
BsrFI	RCCGGY	1 cut(s)	160
BsrI	ACTGG	7 cut(s)	146, 261, 290, 1037, 1314, 1351, 1447
BssAI	RCCGGY	1 cut(s)	160
BssSI	CACGAG	1 cut(s)	999
BssT1I	CCWWGG	5 cut(s)	653, 677, 1020, 1137, 1420
Bst2BI	CACGAG	1 cut(s)	999
Bst2UI	CCWGG	2 cut(s)	578, 1371
Bst4CI	ACNGT	7 cut(s)	313, 331, 358, 423, 1294, 1329, 1561
Bst6I	CTCTTC	2 cut(s)	611, 786
BstBAI	YACGTR	1 cut(s)	1232
BstBI	TTCGAA	1 cut(s)	710
BstC8I	GCNNGC	6 cut(s)	162, 250, 417, 824, 1165, 1227
BstDEI	CTNAG	9 cut(s)	453, 609, 750, 840, 858, 907, 1270, 1276, 1498
BstDSI	CCRYGG	2 cut(s)	1020, 1137
BstENI	CCTNNNNNAGG	1 cut(s)	1368
BstF5I	GGATG	8 cut(s)	17, 129, 367, 719, 953, 1142, 1541, 1651
BstFNI	CGCG	1 cut(s)	177
BstHHI	GCGC	2 cut(s)	1064, 1504
BstMAI	GTCTC	4 cut(s)	457, 613, 910, 1653
BstMCI	CGRYCG	1 cut(s)	165
BstNI	CCWGG	2 cut(s)	578, 1371
BstNSI	RCATGY	1 cut(s)	1171
BstSCI	CCNGG	4 cut(s)	576, 1369, 1728, 1729
BstSFI	CTRYAG	1 cut(s)	1155
BstUI	CGCG	1 cut(s)	177
BstV2I	GAAGAC	1 cut(s)	1074
BstXI	CCANNNNNNTGG	1 cut(s)	1427
BstZI	CGGCCG	1 cut(s)	162
BsuRI	GGCC	8 cut(s)	160, 164, 182, 875, 884, 1025, 1136, 1350
BtgI	CCRYGG	2 cut(s)	1020, 1137
BtsCI	GGATG	8 cut(s)	17, 129, 367, 719, 953, 1142, 1541, 1651
BtsI	GCAGTG	2 cut(s)	1584, 1602
BtsIMutI	CAGTG	7 cut(s)	268, 274, 309, 939, 1566, 1584, 1602
Cac8I	GCNNGC	6 cut(s)	162, 250, 417, 824, 1165, 1227
CaiI	CAGNNNCTG	2 cut(s)	466, 664
CciI	TCATGA	3 cut(s)	409, 583, 1206
CfoI	GCGC	2 cut(s)	1064, 1504
Cfr10I	RCCGGY	1 cut(s)	160
Cfr13I	GGNCC	4 cut(s)	674, 1074, 1182, 1391
Cfr9I	CCCGGG	1 cut(s)	1729
CseI	GACGC	1 cut(s)	308
Csp6I	GTAC	3 cut(s)	765, 1017, 1052
CviQI	GTAC	3 cut(s)	765, 1017, 1052
DdeI	CTNAG	9 cut(s)	453, 609, 750, 840, 858, 907, 1270, 1276, 1498
EaeI	YGGCCR	4 cut(s)	162, 180, 1023, 1134
EagI	CGGCCG	1 cut(s)	162
Eam1104I	CTCTTC	2 cut(s)	611, 786
EarI	CTCTTC	2 cut(s)	611, 786
EciI	GGCGGA	2 cut(s)	710, 1656
Ecl136II	GAGCTC	1 cut(s)	436
EclXI	CGGCCG	1 cut(s)	162
Eco130I	CCWWGG	5 cut(s)	653, 677, 1020, 1137, 1420
Eco24I	GRGCYC	1 cut(s)	438
Eco47I	GGWCC	4 cut(s)	674, 1074, 1182, 1391
Eco52I	CGGCCG	1 cut(s)	162
Eco53kI	GAGCTC	1 cut(s)	436
Eco57I	CTGAAG	1 cut(s)	488
Eco72I	CACGTG	1 cut(s)	1232
Eco88I	CYCGRG	3 cut(s)	800, 900, 1729
EcoICRI	GAGCTC	1 cut(s)	436
EcoNI	CCTNNNNNAGG	1 cut(s)	1368
EcoRI	GAATTC	2 cut(s)	140, 712
EcoRII	CCWGG	2 cut(s)	576, 1369
EcoT14I	CCWWGG	5 cut(s)	653, 677, 1020, 1137, 1420
EcoT22I	ATGCAT	1 cut(s)	519
EcoT38I	GRGCYC	1 cut(s)	438
ErhI	CCWWGG	5 cut(s)	653, 677, 1020, 1137, 1420
Esp3I	CGTCTC	1 cut(s)	1653
FaqI	GGGAC	1 cut(s)	783
FauI	CCCGC	1 cut(s)	1122
FbaI	TGATCA	8 cut(s)	232, 346, 379, 565, 586, 868, 936, 1083
FblI	GTMKAC	1 cut(s)	195
FokI	GGATG	8 cut(s)	24, 136, 374, 706, 960, 1129, 1528, 1638
FriOI	GRGCYC	1 cut(s)	438
FseI	GGCCGGCC	1 cut(s)	164
FspBI	CTAG	4 cut(s)	654, 1164, 1226, 1613
FspI	TGCGCA	1 cut(s)	1063
GlaI	GCGC	2 cut(s)	1063, 1503
GsuI	CTGGAG	1 cut(s)	422
HaeIII	GGCC	8 cut(s)	160, 164, 182, 875, 884, 1025, 1136, 1350
HapII	CCGG	4 cut(s)	132, 161, 339, 1730
HgaI	GACGC	1 cut(s)	308
HhaI	GCGC	2 cut(s)	1064, 1504
Hin6I	GCGC	2 cut(s)	1062, 1502
HinP1I	GCGC	2 cut(s)	1062, 1502
HincII	GTYRAC	3 cut(s)	187, 196, 1538
HindII	GTYRAC	3 cut(s)	187, 196, 1538
HinfI	GANTC	7 cut(s)	29, 35, 406, 457, 796, 803, 903
HpaII	CCGG	4 cut(s)	132, 161, 339, 1730
HphI	GGTGA	4 cut(s)	128, 738, 1300, 1618
Hpy166II	GTNNAC	7 cut(s)	187, 196, 307, 334, 1046, 1319, 1538
Hpy188III	TCNNGA	7 cut(s)	236, 274, 410, 584, 639, 800, 1207
Hpy8I	GTNNAC	7 cut(s)	187, 196, 307, 334, 1046, 1319, 1538
Hpy99I	CGWCG	1 cut(s)	358
HpyAV	CCTTC	4 cut(s)	271, 463, 593, 1091
HpyCH4III	ACNGT	7 cut(s)	313, 331, 358, 423, 1294, 1329, 1561
HpyCH4IV	ACGT	2 cut(s)	1057, 1231
HpyCH4V	TGCA	7 cut(s)	319, 427, 517, 535, 1157, 1196, 1553
HpyF3I	CTNAG	9 cut(s)	453, 609, 750, 840, 858, 907, 1270, 1276, 1498
HpySE526I	ACGT	2 cut(s)	1057, 1231
HspAI	GCGC	2 cut(s)	1062, 1502
KroI	GCCGGC	1 cut(s)	160
KroNI	GCCGGC	1 cut(s)	162
Ksp22I	TGATCA	8 cut(s)	232, 346, 379, 565, 586, 868, 936, 1083
LmnI	GCTCC	3 cut(s)	441, 934, 1285
LweI	GCATC	4 cut(s)	504, 526, 938, 1151
MaeI	CTAG	4 cut(s)	654, 1164, 1226, 1613
MaeII	ACGT	2 cut(s)	1057, 1231
MaeIII	GTNAC	4 cut(s)	325, 487, 1170, 1294
MboII	GAAGA	8 cut(s)	40, 193, 628, 695, 708, 803, 1079, 1232
MhlI	GDGCHC	1 cut(s)	438
MlsI	TGGCCA	1 cut(s)	1136
MluCI	AATT	5 cut(s)	140, 428, 712, 1219, 1454
MluNI	TGGCCA	1 cut(s)	1136
MlyI	GAGTC	3 cut(s)	805, 812, 912
MmeI	TCCRAC	1 cut(s)	1619
Mox20I	TGGCCA	1 cut(s)	1136
Mph1103I	ATGCAT	1 cut(s)	519
MroNI	GCCGGC	1 cut(s)	160
MscI	TGGCCA	1 cut(s)	1136
Msp20I	TGGCCA	1 cut(s)	1136
MspA1I	CMGCKG	5 cut(s)	563, 1117, 1160, 1360, 1577
MspI	CCGG	4 cut(s)	132, 161, 339, 1730
MspR9I	CCNGG	4 cut(s)	578, 1371, 1730, 1731
Mva1269I	GAATGC	1 cut(s)	454
MvaI	CCWGG	2 cut(s)	578, 1371
MvnI	CGCG	1 cut(s)	177
NaeI	GCCGGC	1 cut(s)	162
NciI	CCSGG	2 cut(s)	1730, 1731
NcoI	CCATGG	2 cut(s)	1020, 1137
NgoMIV	GCCGGC	1 cut(s)	160
NheI	GCTAGC	2 cut(s)	1163, 1225
NlaIV	GGNNCC	3 cut(s)	651, 675, 1183
NmeAIII	GCCGAG	1 cut(s)	21
NmuCI	GTSAC	2 cut(s)	487, 1294
NsbI	TGCGCA	1 cut(s)	1063
NsiI	ATGCAT	1 cut(s)	519
NspI	RCATGY	1 cut(s)	1171
NspV	TTCGAA	1 cut(s)	710
PaeR7I	CTCGAG	1 cut(s)	900
PagI	TCATGA	3 cut(s)	409, 583, 1206
PctI	GAATGC	1 cut(s)	454
PdiI	GCCGGC	1 cut(s)	162
PfeI	GAWTC	4 cut(s)	29, 35, 406, 457
PflFI	GACNNNGTC	1 cut(s)	191
PleI	GAGTC	3 cut(s)	804, 811, 911
PmaCI	CACGTG	1 cut(s)	1232
PmlI	CACGTG	1 cut(s)	1232
PpsI	GAGTC	3 cut(s)	804, 811, 911
Ppu21I	YACGTR	1 cut(s)	1232
Psp124BI	GAGCTC	1 cut(s)	438
Psp1406I	AACGTT	1 cut(s)	1057
Psp6I	CCWGG	2 cut(s)	576, 1369
PspCI	CACGTG	1 cut(s)	1232
PspGI	CCWGG	2 cut(s)	576, 1369
PspN4I	GGNNCC	3 cut(s)	651, 675, 1183
PspPI	GGNCC	4 cut(s)	674, 1074, 1182, 1391
PspXI	VCTCGAGB	1 cut(s)	900
PstI	CTGCAG	1 cut(s)	1159
PstNI	CAGNNNCTG	2 cut(s)	466, 664
PsyI	GACNNNGTC	1 cut(s)	191
PvuII	CAGCTG	3 cut(s)	563, 1360, 1577
RigI	GGCCGGCC	1 cut(s)	164
RsaI	GTAC	3 cut(s)	766, 1018, 1053
RsaNI	GTAC	3 cut(s)	765, 1017, 1052
SacI	GAGCTC	1 cut(s)	438
SalI	GTCGAC	1 cut(s)	194
Sau96I	GGNCC	4 cut(s)	674, 1074, 1182, 1391
ScaI	AGTACT	1 cut(s)	766
SchI	GAGTC	3 cut(s)	805, 812, 912
ScrFI	CCNGG	4 cut(s)	578, 1371, 1730, 1731
SduI	GDGCHC	1 cut(s)	438
SfaNI	GCATC	4 cut(s)	504, 526, 938, 1151
SfcI	CTRYAG	1 cut(s)	1155
SfiI	GGCCNNNNNGGCC	1 cut(s)	881
Sfr274I	CTCGAG	1 cut(s)	900
SfuI	TTCGAA	1 cut(s)	710
SinI	GGWCC	4 cut(s)	674, 1074, 1182, 1391
SlaI	CTCGAG	1 cut(s)	900
SmaI	CCCGGG	1 cut(s)	1731
SmlI	CTYRAG	1 cut(s)	900
SmoI	CTYRAG	1 cut(s)	900
Sse9I	AATT	5 cut(s)	140, 428, 712, 1219, 1454
SspI	AATATT	1 cut(s)	595
SspMI	CTAG	4 cut(s)	654, 1164, 1226, 1613
SstI	GAGCTC	1 cut(s)	438
StyD4I	CCNGG	4 cut(s)	576, 1369, 1728, 1729
StyI	CCWWGG	5 cut(s)	653, 677, 1020, 1137, 1420
TaaI	ACNGT	7 cut(s)	313, 331, 358, 423, 1294, 1329, 1561
TaiI	ACGT	2 cut(s)	1060, 1234
TaqI	TCGA	6 cut(s)	195, 353, 710, 901, 913, 1493
TaqII	GACCGA	1 cut(s)	506
TasI	AATT	5 cut(s)	140, 428, 712, 1219, 1454
TatI	WGTACW	1 cut(s)	764
TauI	GCSGC	5 cut(s)	560, 1130, 1163, 1610, 1622
TfiI	GAWTC	4 cut(s)	29, 35, 406, 457
TscAI	CASTG	7 cut(s)	268, 274, 316, 946, 1566, 1591, 1609
TseFI	GTSAC	2 cut(s)	487, 1294
Tsp45I	GTSAC	2 cut(s)	487, 1294
TspDTI	ATGAA	5 cut(s)	398, 461, 708, 1223, 1502
TspGWI	ACGGA	1 cut(s)	57
TspMI	CCCGGG	1 cut(s)	1729
TspRI	CASTG	7 cut(s)	268, 274, 316, 946, 1566, 1591, 1609
Tth111I	GACNNNGTC	1 cut(s)	191
VpaK11BI	GGWCC	4 cut(s)	674, 1074, 1182, 1391
XagI	CCTNNNNNAGG	1 cut(s)	1368
XapI	RAATTY	3 cut(s)	140, 712, 1454
XceI	RCATGY	1 cut(s)	1171
XhoI	CTCGAG	1 cut(s)	900
XmaI	CCCGGG	1 cut(s)	1729
XmaJI	CCTAGG	1 cut(s)	653
XmiI	GTMKAC	1 cut(s)	195
XspI	CTAG	4 cut(s)	654, 1164, 1226, 1613
ZrmI	AGTACT	1 cut(s)	766
Zsp2I	ATGCAT	1 cut(s)	519

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.