Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007021

Physical Location & Seq

Genomic Coordinates Reverse (-)

40512430 .. 40515449

Gene Structure 3020 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1324500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGGACAAAGGATGGGGGCTCACCCTTGATTCTGACTCCTGCGGCTTCCTCCTCAACAAGCCGCCCACCGCGGCGGTTAAGCTCGACCACAACCACAATAACAACAAGAGAAGCAGTTTTTTTAGTGGGGAAAGGATGTTTTCGGGAATGGAATTCCCTGTCAAACTTGGCAGCCGGGAAGAGCAGACTGCATCACAGCCGTCCAATGACAACGATCGGATGGTTGTGGATGAGGTGGACTTCTTTTCTGATCGTAAGAACAAGCTCAAAAGCAATAGTACTGATGATCATCATCAGGATTCGAGATCCAATGATGCAATGAGTGTCAAGAAGGAGAATTGGACTGGTTTGGATGTTAATACTGGATTGCACCTTGTTACTGCGAACACCGGAAGTGATCAGTCGATGGTTGATGATGGGACTTCTTCTGATCGTGCGGATGACAAAAGAGCAAAGAATCATGAGCTGGCACAGTTGCAAGTGGAGCTCCAACGTATGAATGCTGAAAACCAGAGGCTGAAAGAGATGCTAGGTCAAGTGACCAACAACTACAGTGCATTACAGATGCATCTTGCTGCAGTTGTACAACAACAACATAATCACACTACTGCAGCTCCAGCTGATCAGAGCTCACAATTACACCATGATCAGAATGCTGAGGCAAAGGCTGATCAAGGTAAGAAACAAGGGTTGGTGCCAAGACAATTTCTAAATTTGGGTCCGAGAGCCACAGCCGAGGCGACTGATGACCAAGTTTCCAATAGTTCATCGGAAGCGAGAACCGGATCAGCTTCACCACCTCAAAACATCAGTGAAGTTGCATCACCGAAAAACGGTCAGATTGGTCCGTTGGATCCGGAAAACTCTAATACTTTCCGTGACGGGAAAAGAGTTGGCAGGGATGAGAGTCCTGAATCTGAATCAGAAGGATGGGTGCCCGACAAGGTCCCCAAGCTCAACAATTCTGCGGCTCCTAAGCCAATTGATCAATCAACCGAGGCTACCATGAGGAAAGCCCGCGTCTCCGTTCGAGCCAGATCAGAAGCCTCCATGATTACTGATGGGTGTCAATGGAGAAAGTATGGACAGAAGATGGCAAAAGGGAACCCATGTCCTCGTGCATATTATAGGTGCACCATGGCTGTAGGCTGTCCTGTTCGCAAACAAGTACAACGTTGTGCGGAGGACAGAACCATACTGATCACAACATATGAAGGCAATCACAATCACCCACTGCCCCCCGCTGCAATGGCCATGGCATCAACCACCACGGCAGCTGCCAGCATGTTGCTTTCGGGGTCCATGTCAAGTGCGGACGGGATGAATATGAACCCTAATAATTTGCTAGCCAGAGCAATTCTTCCATGCTCATCAAGTGTTGCCACAATCTCAGCCTCAGCTCCATTTCCAACTGTTACATTGGACCTTACCACATCGCCATCACATGTCCAAAGACCAAACACCCCATTTCAAGTCCCTAATTTCCCAGGGCAACCCCCGAGTTTTGGGGCAGCTTCACATCAACTGCCTCAAGTTTTTGGGCAAGCCCTTTACAACCAGTCTAAGTTTTCAGGACTTCAGCTGTCCCAGGATACAATGGGGTCTACTAGTTCACAAAAACAAATGCAGCAAACGCAATCTTCCTCCTTTGCTGACACAGTGAGTGCCATCACGGCTGATCCGAGCTTCACTGCTGCTCTCGCGGCCGCTATAACCTCGATCATTGGCGGAGGAGCTCATCCGAGCAACACCACCACCAACAGCGCCTCCCCCATGTCCAACAACAGCAGCGGTGCCAACAACAACAACAAGATTAGTGGTTTCCCGGGAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010033 GO:0010036 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016036 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042221 GO:0042493 GO:0042594 GO:0044212 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071496 GO:0080029 GO:0080090 GO:0080169 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

612

Amino Acids

65.65

Weight (kDa)

6.61

Isoelectric Point (pI)

46.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	354 - 411	2.6e-25	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007021)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62300 AT4G04450 AT4G04450 AT4G22070 AT4G22070 AT4G22070 AT4G22070
fragaria_vesca	FvH4_3g01700 FvH4_3g01700
malus_domestica	MD05G1349800.v1.1 MD10G1324500.v1.1
prunus_persica	Prupe.4G017600_v2.0.a1 Prupe.4G017600_v2.0.a1
pyrus_communis	pycom05g31740 pycom10g27490
rosa_chinensis	RchiOBHm_Chr5g0002561
rosa_laevigata	RLG00000031039
rosa_multiflora	Rmu_sc0010731.1_g000002
rosa_roxburghii	Rroxscaffold_1G00073350
rosa_rugosa	Rorug04G0396900
rosa_samantha	Rh5AG020300 Rh5BG023000 Rh5CG022300 Rh5DG021400
rosa_wichuraiana	Rw5G001870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	694, 934, 1793
AccI	GTMKAC	1 cut(s)	1604
AccII	CGCG	3 cut(s)	71, 1020, 1703
AccIII	TCCGGA	1 cut(s)	856
AclI	AACGTT	1 cut(s)	1174
AclWI	GGATC	5 cut(s)	300, 793, 848, 861, 1673
AcoI	YGGCCR	2 cut(s)	1251, 1704
AcsI	RAATTY	2 cut(s)	152, 712
AcuI	CTGAAG	1 cut(s)	1562
AfaI	GTAC	3 cut(s)	280, 585, 1170
AfiI	CCNNNNNNNGG	2 cut(s)	833, 1505
AflIII	ACRYGT	1 cut(s)	1444
AgsI	TTSAA	1 cut(s)	1472
AhlI	ACTAGT	1 cut(s)	1607
AjnI	CCWGG	2 cut(s)	1486, 1587
AjuI	GAANNNNNNNTTGG	2 cut(s)	674, 706
Alw21I	GWGCWC	4 cut(s)	489, 632, 1136, 1738
Alw26I	GTCTC	1 cut(s)	1027
Alw44I	GTGCAC	1 cut(s)	1132
AlwI	GGATC	5 cut(s)	300, 793, 848, 861, 1673
AlwNI	CAGNNNCTG	1 cut(s)	517
Ama87I	CYCGRG	2 cut(s)	1498, 1825
Aor13HI	TCCGGA	1 cut(s)	856
AoxI	GGCC	2 cut(s)	1251, 1704
ApaLI	GTGCAC	1 cut(s)	1132
ApoI	RAATTY	2 cut(s)	152, 712
AspLEI	GCGC	1 cut(s)	1766
AspS9I	GGNCC	5 cut(s)	719, 845, 946, 1299, 1423
AsuC2I	CCSGG	3 cut(s)	176, 1826, 1827
AsuHPI	GGTGA	4 cut(s)	13, 786, 816, 1220
AsuNHI	GCTAGC	1 cut(s)	1345
AvaI	CYCGRG	2 cut(s)	1498, 1825
AvaII	GGWCC	5 cut(s)	719, 845, 946, 1299, 1423
BaeGI	GKGCMC	2 cut(s)	939, 1136
BalI	TGGCCA	1 cut(s)	1253
BamHI	GGATCC	1 cut(s)	853
BanI	GGYRCC	3 cut(s)	694, 934, 1793
BanII	GRGCYC	4 cut(s)	21, 489, 632, 1738
BauI	CACGAG	1 cut(s)	1116
Bbv12I	GWGCWC	4 cut(s)	489, 632, 1136, 1738
BbvCI	CCTCAGC	2 cut(s)	657, 1396
BccI	CCATC	9 cut(s)	6, 214, 400, 410, 924, 1055, 1087, 1447, 1676
BceAI	ACGGC	3 cut(s)	184, 1287, 1689
BciT130I	CCWGG	2 cut(s)	1488, 1589
BciVI	GTATCC	1 cut(s)	1585
BclI	TGATCA	7 cut(s)	286, 397, 622, 646, 670, 985, 1200
BcnI	CCSGG	3 cut(s)	176, 1826, 1827
BcoDI	GTCTC	1 cut(s)	1027
BcuI	ACTAGT	1 cut(s)	1607
BfaI	CTAG	3 cut(s)	530, 1346, 1608
BfmI	CTRYAG	4 cut(s)	550, 576, 609, 1143
BfoI	RGCGCY	1 cut(s)	1767
BfuI	GTATCC	1 cut(s)	1585
BglI	GCCNNNNNGGC	1 cut(s)	1673
BmcAI	AGTACT	1 cut(s)	280
Bme1390I	CCNGG	5 cut(s)	176, 1488, 1589, 1826, 1827
Bme18I	GGWCC	5 cut(s)	719, 845, 946, 1299, 1423
BmeT110I	CYCGRG	2 cut(s)	1498, 1825
BmgT120I	GGNCC	5 cut(s)	719, 845, 946, 1299, 1423
BmiI	GGNNCC	9 cut(s)	696, 720, 855, 936, 948, 972, 1106, 1300, 1795
BmrFI	CCNGG	5 cut(s)	176, 1488, 1589, 1826, 1827
BmsI	GCATC	7 cut(s)	200, 304, 516, 555, 577, 830, 1268
BmtI	GCTAGC	1 cut(s)	1349
BpmI	CTGGAG	1 cut(s)	600
Bpu10I	CCTNAGC	3 cut(s)	657, 975, 1396
BpuEI	CTTGAG	1 cut(s)	1515
BpuMI	CCSGG	3 cut(s)	176, 1826, 1827
BsaBI	GATNNNNATC	2 cut(s)	288, 291
BsaWI	WCCGGW	3 cut(s)	389, 782, 856
BsaXI	ACNNNNNCTCC	4 cut(s)	598, 628, 1751, 1781
Bsc4I	CCNNNNNNNGG	2 cut(s)	833, 1505
Bse1I	ACTGG	3 cut(s)	349, 367, 1558
Bse3DI	GCAATG	2 cut(s)	324, 1254
Bse8I	GATNNNNATC	2 cut(s)	288, 291
BseAI	TCCGGA	1 cut(s)	856
BseBI	CCWGG	2 cut(s)	1488, 1589
BseJI	GATNNNNATC	2 cut(s)	288, 291
BseLI	CCNNNNNNNGG	2 cut(s)	833, 1505
BseMI	GCAATG	2 cut(s)	324, 1254
BseMII	CTCAG	3 cut(s)	648, 1404, 1410
BseNI	ACTGG	3 cut(s)	349, 367, 1558
BseRI	GAGGAG	2 cut(s)	41, 1746
BseSI	GKGCMC	2 cut(s)	939, 1136
BseX3I	CGGCCG	1 cut(s)	1704
Bsh1236I	CGCG	3 cut(s)	71, 1020, 1703
Bsh1285I	CGRYCG	2 cut(s)	217, 1707
BshFI	GGCC	2 cut(s)	1253, 1706
BshNI	GGYRCC	3 cut(s)	694, 934, 1793
BsiEI	CGRYCG	2 cut(s)	217, 1707
BsiHKAI	GWGCWC	4 cut(s)	489, 632, 1136, 1738
BsiHKCI	CYCGRG	2 cut(s)	1498, 1825
BsiSI	CCGG	5 cut(s)	175, 390, 783, 857, 1826
BslFI	GGGAC	4 cut(s)	433, 932, 1460, 1570
BslI	CCNNNNNNNGG	2 cut(s)	833, 1505
BsmAI	GTCTC	1 cut(s)	1027
BsmBI	CGTCTC	1 cut(s)	1027
BsmFI	GGGAC	4 cut(s)	433, 932, 1460, 1570
BsmI	GAATGC	2 cut(s)	505, 658
BsnI	GGCC	2 cut(s)	1253, 1706
BsoBI	CYCGRG	2 cut(s)	1498, 1825
Bsp1286I	GDGCHC	6 cut(s)	21, 489, 632, 939, 1136, 1738
Bsp13I	TCCGGA	1 cut(s)	856
Bsp1407I	TGTACA	1 cut(s)	583
Bsp19I	CCATGG	2 cut(s)	1137, 1254
BspANI	GGCC	2 cut(s)	1253, 1706
BspCNI	CTCAG	3 cut(s)	649, 1403, 1409
BspEI	TCCGGA	1 cut(s)	856
BspFNI	CGCG	3 cut(s)	71, 1020, 1703
BspHI	TCATGA	1 cut(s)	460
BspLI	GGNNCC	9 cut(s)	696, 720, 855, 936, 948, 972, 1106, 1300, 1795
BspMAI	CTGCAG	2 cut(s)	580, 613
BspOI	GCTAGC	1 cut(s)	1349
BspPI	GGATC	5 cut(s)	300, 793, 848, 861, 1673
BspQI	GCTCTTC	1 cut(s)	174
BspT107I	GGYRCC	3 cut(s)	694, 934, 1793
BsrDI	GCAATG	2 cut(s)	324, 1254
BsrGI	TGTACA	1 cut(s)	583
BsrI	ACTGG	3 cut(s)	349, 367, 1558
BssSI	CACGAG	1 cut(s)	1116
BssT1I	CCWWGG	2 cut(s)	1137, 1254
Bst2BI	CACGAG	1 cut(s)	1116
Bst2UI	CCWGG	2 cut(s)	1488, 1589
Bst4CI	ACNGT	5 cut(s)	474, 554, 836, 1414, 1660
Bst6I	CTCTTC	1 cut(s)	174
BstAUI	TGTACA	1 cut(s)	583
BstC8I	GCNNGC	5 cut(s)	468, 1018, 1282, 1347, 1545
BstDEI	CTNAG	5 cut(s)	657, 975, 1390, 1396, 1563
BstDSI	CCRYGG	4 cut(s)	69, 1137, 1254, 1269
BstFNI	CGCG	3 cut(s)	71, 1020, 1703
BstH2I	RGCGCY	1 cut(s)	1767
BstHHI	GCGC	1 cut(s)	1766
BstMAI	GTCTC	1 cut(s)	1027
BstMCI	CGRYCG	2 cut(s)	217, 1707
BstMWI	GCNNNNNNNGC	7 cut(s)	484, 617, 1250, 1633, 1673, 1700, 1703
BstNI	CCWGG	2 cut(s)	1488, 1589
BstNSI	RCATGY	2 cut(s)	1288, 1448
BstSCI	CCNGG	5 cut(s)	174, 1486, 1587, 1824, 1825
BstSFI	CTRYAG	4 cut(s)	550, 576, 609, 1143
BstSLI	GKGCMC	2 cut(s)	939, 1136
BstUI	CGCG	3 cut(s)	71, 1020, 1703
BstX2I	RGATCY	2 cut(s)	305, 853
BstYI	RGATCY	2 cut(s)	305, 853
BstZI	CGGCCG	1 cut(s)	1704
BsuI	GTATCC	1 cut(s)	1585
BsuRI	GGCC	2 cut(s)	1253, 1706
BtgI	CCRYGG	4 cut(s)	69, 1137, 1254, 1269
BtgZI	GCGATG	1 cut(s)	1419
BtsI	GCAGTG	2 cut(s)	1232, 1689
BtsIMutI	CAGTG	5 cut(s)	559, 817, 1232, 1665, 1689
Cac8I	GCNNGC	5 cut(s)	468, 1018, 1282, 1347, 1545
CaiI	CAGNNNCTG	1 cut(s)	517
CciI	TCATGA	1 cut(s)	460
CciNI	GCGGCCGC	1 cut(s)	1704
CfoI	GCGC	1 cut(s)	1766
Cfr13I	GGNCC	5 cut(s)	719, 845, 946, 1299, 1423
Cfr42I	CCGCGG	1 cut(s)	72
Cfr9I	CCCGGG	1 cut(s)	1825
CseI	GACGC	1 cut(s)	1009
Csp6I	GTAC	3 cut(s)	279, 584, 1169
CspCI	CAANNNNNGTGG	2 cut(s)	1798, 1833
CviQI	GTAC	3 cut(s)	279, 584, 1169
DdeI	CTNAG	5 cut(s)	657, 975, 1390, 1396, 1563
EaeI	YGGCCR	2 cut(s)	1251, 1704
EagI	CGGCCG	1 cut(s)	1704
Eam1104I	CTCTTC	1 cut(s)	174
EarI	CTCTTC	1 cut(s)	174
EciI	GGCGGA	1 cut(s)	1743
Ecl136II	GAGCTC	3 cut(s)	487, 630, 1736
EclXI	CGGCCG	1 cut(s)	1704
Eco130I	CCWWGG	2 cut(s)	1137, 1254
Eco24I	GRGCYC	4 cut(s)	21, 489, 632, 1738
Eco47I	GGWCC	5 cut(s)	719, 845, 946, 1299, 1423
Eco52I	CGGCCG	1 cut(s)	1704
Eco53kI	GAGCTC	3 cut(s)	487, 630, 1736
Eco57I	CTGAAG	1 cut(s)	1562
Eco88I	CYCGRG	2 cut(s)	1498, 1825
EcoICRI	GAGCTC	3 cut(s)	487, 630, 1736
EcoO109I	RGGNCCY	1 cut(s)	946
EcoRI	GAATTC	1 cut(s)	152
EcoRII	CCWGG	2 cut(s)	1486, 1587
EcoT14I	CCWWGG	2 cut(s)	1137, 1254
EcoT22I	ATGCAT	1 cut(s)	570
EcoT38I	GRGCYC	4 cut(s)	21, 489, 632, 1738
ErhI	CCWWGG	2 cut(s)	1137, 1254
Esp3I	CGTCTC	1 cut(s)	1027
FaqI	GGGAC	4 cut(s)	433, 932, 1460, 1570
FauI	CCCGC	2 cut(s)	1025, 1249
FauNDI	CATATG	1 cut(s)	1210
FbaI	TGATCA	7 cut(s)	286, 397, 622, 646, 670, 985, 1200
FblI	GTMKAC	1 cut(s)	1604
FriOI	GRGCYC	4 cut(s)	21, 489, 632, 1738
FspBI	CTAG	3 cut(s)	530, 1346, 1608
GlaI	GCGC	1 cut(s)	1765
GsuI	CTGGAG	1 cut(s)	600
HaeII	RGCGCY	1 cut(s)	1767
HaeIII	GGCC	2 cut(s)	1253, 1706
HapII	CCGG	5 cut(s)	175, 390, 783, 857, 1826
HgaI	GACGC	1 cut(s)	1009
HhaI	GCGC	1 cut(s)	1766
Hin6I	GCGC	1 cut(s)	1764
HinP1I	GCGC	1 cut(s)	1764
HinfI	GANTC	7 cut(s)	29, 35, 299, 457, 907, 914, 920
HpaII	CCGG	5 cut(s)	175, 390, 783, 857, 1826
HphI	GGTGA	4 cut(s)	13, 786, 816, 1220
Hpy166II	GTNNAC	4 cut(s)	238, 1134, 1605, 1613
Hpy188III	TCNNGA	8 cut(s)	144, 296, 303, 328, 461, 857, 911, 1572
Hpy8I	GTNNAC	4 cut(s)	238, 1134, 1605, 1613
HpyAV	CCTTC	3 cut(s)	325, 920, 1208
HpyCH4III	ACNGT	5 cut(s)	474, 554, 836, 1414, 1660
HpyCH4IV	ACGT	2 cut(s)	493, 1174
HpyF10VI	GCNNNNNNNGC	7 cut(s)	484, 617, 1250, 1633, 1673, 1700, 1703
HpyF3I	CTNAG	5 cut(s)	657, 975, 1390, 1396, 1563
HpySE526I	ACGT	2 cut(s)	493, 1174
HspAI	GCGC	1 cut(s)	1764
Kpn2I	TCCGGA	1 cut(s)	856
Ksp22I	TGATCA	7 cut(s)	286, 397, 622, 646, 670, 985, 1200
KspI	CCGCGG	1 cut(s)	72
LguI	GCTCTTC	1 cut(s)	174
LmnI	GCTCC	6 cut(s)	484, 492, 619, 976, 1405, 1733
LweI	GCATC	7 cut(s)	200, 304, 516, 555, 577, 830, 1268
MaeI	CTAG	3 cut(s)	530, 1346, 1608
MaeII	ACGT	2 cut(s)	493, 1174
MaeIII	GTNAC	4 cut(s)	376, 538, 878, 1414
MboII	GAAGA	5 cut(s)	191, 417, 1102, 1352, 1632
MfeI	CAATTG	1 cut(s)	981
MflI	RGATCY	2 cut(s)	305, 853
MhlI	GDGCHC	6 cut(s)	21, 489, 632, 939, 1136, 1738
MlsI	TGGCCA	1 cut(s)	1253
MluNI	TGGCCA	1 cut(s)	1253
MlyI	GAGTC	2 cut(s)	29, 916
MmeI	TCCRAC	4 cut(s)	514, 831, 1433, 1803
Mox20I	TGGCCA	1 cut(s)	1253
Mph1103I	ATGCAT	1 cut(s)	570
MroI	TCCGGA	1 cut(s)	856
MscI	TGGCCA	1 cut(s)	1253
MseI	TTAA	3 cut(s)	78, 357, 1834
Msp20I	TGGCCA	1 cut(s)	1253
MspA1I	CMGCKG	6 cut(s)	71, 620, 1244, 1277, 1582, 1791
MspI	CCGG	5 cut(s)	175, 390, 783, 857, 1826
MspR9I	CCNGG	5 cut(s)	176, 1488, 1589, 1826, 1827
MunI	CAATTG	1 cut(s)	981
Mva1269I	GAATGC	2 cut(s)	505, 658
MvaI	CCWGG	2 cut(s)	1488, 1589
MvnI	CGCG	3 cut(s)	71, 1020, 1703
MwoI	GCNNNNNNNGC	7 cut(s)	484, 617, 1250, 1633, 1673, 1700, 1703
NciI	CCSGG	3 cut(s)	176, 1826, 1827
NcoI	CCATGG	2 cut(s)	1137, 1254
NdeI	CATATG	1 cut(s)	1210
NheI	GCTAGC	1 cut(s)	1345
NlaIV	GGNNCC	9 cut(s)	696, 720, 855, 936, 948, 972, 1106, 1300, 1795
NmeAIII	GCCGAG	1 cut(s)	760
NmuCI	GTSAC	2 cut(s)	538, 878
NotI	GCGGCCGC	1 cut(s)	1704
NsiI	ATGCAT	1 cut(s)	570
NspI	RCATGY	2 cut(s)	1288, 1448
PagI	TCATGA	1 cut(s)	460
PasI	CCCWGGG	1 cut(s)	1487
PciI	ACATGT	1 cut(s)	1444
PciSI	GCTCTTC	1 cut(s)	174
PcsI	WCGNNNNNNNCGW	1 cut(s)	1679
PctI	GAATGC	2 cut(s)	505, 658
PfeI	GAWTC	5 cut(s)	29, 299, 457, 914, 920
PflFI	GACNNNGTC	1 cut(s)	944
Ple19I	CGATCG	1 cut(s)	217
PleI	GAGTC	2 cut(s)	29, 915
PpsI	GAGTC	2 cut(s)	29, 915
PpuMI	RGGWCCY	1 cut(s)	946
PscI	ACATGT	1 cut(s)	1444
Psp124BI	GAGCTC	3 cut(s)	489, 632, 1738
Psp1406I	AACGTT	1 cut(s)	1174
Psp5II	RGGWCCY	1 cut(s)	946
Psp6I	CCWGG	2 cut(s)	1486, 1587
PspGI	CCWGG	2 cut(s)	1486, 1587
PspN4I	GGNNCC	9 cut(s)	696, 720, 855, 936, 948, 972, 1106, 1300, 1795
PspPI	GGNCC	5 cut(s)	719, 845, 946, 1299, 1423
PspPPI	RGGWCCY	1 cut(s)	946
PstI	CTGCAG	2 cut(s)	580, 613
PstNI	CAGNNNCTG	1 cut(s)	517
PsuI	RGATCY	2 cut(s)	305, 853
PsyI	GACNNNGTC	1 cut(s)	944
PvuI	CGATCG	1 cut(s)	217
PvuII	CAGCTG	3 cut(s)	620, 1277, 1582
RsaI	GTAC	3 cut(s)	280, 585, 1170
RsaNI	GTAC	3 cut(s)	279, 584, 1169
SacI	GAGCTC	3 cut(s)	489, 632, 1738
SacII	CCGCGG	1 cut(s)	72
SapI	GCTCTTC	1 cut(s)	174
SaqAI	TTAA	3 cut(s)	78, 357, 1834
Sau96I	GGNCC	5 cut(s)	719, 845, 946, 1299, 1423
ScaI	AGTACT	1 cut(s)	280
SchI	GAGTC	2 cut(s)	29, 916
ScrFI	CCNGG	5 cut(s)	176, 1488, 1589, 1826, 1827
SduI	GDGCHC	6 cut(s)	21, 489, 632, 939, 1136, 1738
SfaNI	GCATC	7 cut(s)	200, 304, 516, 555, 577, 830, 1268
SfcI	CTRYAG	4 cut(s)	550, 576, 609, 1143
Sfr303I	CCGCGG	1 cut(s)	72
SgrBI	CCGCGG	1 cut(s)	72
SinI	GGWCC	5 cut(s)	719, 845, 946, 1299, 1423
SmaI	CCCGGG	1 cut(s)	1827
SmlI	CTYRAG	1 cut(s)	1530
SmoI	CTYRAG	1 cut(s)	1530
SpeI	ACTAGT	1 cut(s)	1607
SspMI	CTAG	3 cut(s)	530, 1346, 1608
SstI	GAGCTC	3 cut(s)	489, 632, 1738
StyD4I	CCNGG	5 cut(s)	174, 1486, 1587, 1824, 1825
StyI	CCWWGG	2 cut(s)	1137, 1254
TaaI	ACNGT	5 cut(s)	474, 554, 836, 1414, 1660
TaiI	ACGT	2 cut(s)	496, 1177
TaqI	TCGA	5 cut(s)	84, 302, 404, 1030, 1718
TatI	WGTACW	3 cut(s)	278, 583, 1168
TauI	GCSGC	6 cut(s)	45, 64, 74, 971, 1706, 1709
TfiI	GAWTC	5 cut(s)	29, 299, 457, 914, 920
Tru1I	TTAA	3 cut(s)	78, 357, 1834
Tru9I	TTAA	3 cut(s)	78, 357, 1834
TscAI	CASTG	5 cut(s)	559, 817, 1239, 1665, 1696
TseFI	GTSAC	2 cut(s)	538, 878
Tsp45I	GTSAC	2 cut(s)	538, 878
TspDTI	ATGAA	5 cut(s)	512, 756, 1227, 1337, 1343
TspGWI	ACGGA	3 cut(s)	837, 866, 1015
TspMI	CCCGGG	1 cut(s)	1825
TspRI	CASTG	5 cut(s)	559, 817, 1239, 1665, 1696
Tth111I	GACNNNGTC	1 cut(s)	944
VneI	GTGCAC	1 cut(s)	1132
VpaK11BI	GGWCC	5 cut(s)	719, 845, 946, 1299, 1423
XapI	RAATTY	2 cut(s)	152, 712
XceI	RCATGY	2 cut(s)	1288, 1448
XmaI	CCCGGG	1 cut(s)	1825
XmiI	GTMKAC	1 cut(s)	1604
XspI	CTAG	3 cut(s)	530, 1346, 1608
ZrmI	AGTACT	1 cut(s)	280
Zsp2I	ATGCAT	1 cut(s)	570

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.