AT4G05320

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0017475
Physical Location & Seq
Forward (+)
2717977 .. 2720308
2332 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G05320.5

Sequence Viewer

Length: 1146 bp
ATGCAGATCTTTGTTAAGACTCTCACCGGAAAGACAATCACCCTCGAGGTGGAAAGCTCCGACACCATCGACAACGTTAAGGCCAAGATCCAGGATAAGGAGGGCATTCCTCCGGATCAGCAGAGGCTTATTTTCGCCGGCAAGCAGCTAGAGGATGGCCGTACGTTGGCTGATTACAATATCCAGAAGGAATCCACCCTCCACTTGGTCCTCAGGCTCCGTGGTGGTATGCAGATTTTCGTTAAAACCCTAACGGGAAAGACGATTACTCTTGAGGTGGAGAGTTCTGACACCATCGACAACGTCAAGGCCAAGATCCAAGACAAAGAGGGTATTCCTCCGGACCAGCAGAGGCTGATCTTCGCCGGAAAGCAGTTGGAGGATGGCAGAACTCTTGCTGACTACAATATCCAGAAGGAGTCCACCCTTCATCTTGTTCTCAGGCTCCGTGGTGGTATGCAGATTTTCGTTAAGACGTTGACTGGGAAAACTATCACTTTGGAGGTGGAGAGTTCTGACACCATTGATAACGTGAAAGCCAAGATCCAGGACAAAGAGGGTATCCCACCGGACCAGCAGAGATTGATCTTCGCCGGAAAGCAACTTGAAGATGGAAGAACTTTGGCTGACTACAACATTCAGAAGGAGTCCACACTTCACTTGGTCTTGCGTCTGCGTGGAGGTATGCAGATCTTCGTGAAGACTCTCACCGGAAAGACTATCACTTTGGAGGTAGAGAGCTCTGACACCATTGACAACGTGAAGGCCAAGATCCAGGATAAGGAAGGAATCCCTCCGGACCAGCAGAGGTTGATCTTTGCCGGAAAACAATTGGAGGATGGTCGTACTTTGGCGGATTACAACATCCAGAAGGAGTCGACCCTTCACTTGGTGTTGCGTCTGCGTGGAGGTATGCAGATCTTCGTCAAGACTTTGACCGGAAAGACCATCACCCTTGAAGTGGAAAGCTCCGACACCATTGACAACGTCAAGGCCAAGATCCAGGACAAGGAAGGTATTCCTCCGGACCAGCAGCGTCTCATCTTCGCTGGAAAGCAGCTTGAGGATGGACGTACTTTGGCCGACTACAACATCCAGAAGGAGTCTACTCTTCACTTGGTCCTGCGTCTTCGTGGTGGTTTCTAA

Protein Analysis

381

Amino Acids

42.7

Weight (kDa)

7.0

Isoelectric Point (pI)

29.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 5.8e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 74 7.4e-32 Ubiquitin family
Rad60-SLD PF11976 77 - 147 5.8e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 79 - 150 7.4e-32 Ubiquitin family
Rad60-SLD PF11976 153 - 223 5.8e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 155 - 226 7.4e-32 Ubiquitin family
Rad60-SLD PF11976 229 - 299 5.8e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 231 - 302 7.4e-32 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017475)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05320 AT4G05320 AT4G05320
fragaria_vesca FvH4_3g21751 FvH4_3g21751 FvH4_3g21853
malus_domestica MD03G1211300.v1.1
prunus_persica Prupe.4G025900_v2.0.a1 Prupe.4G025900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0003941
rosa_samantha Rh5AG255500
rosa_wichuraiana Rw3G014130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 44
AccI GTMKAC 2 cut(s) 878, 1106
AccIII TCCGGA 4 cut(s) 112, 340, 796, 1024
AciI CCGC 1 cut(s) 854
AclI AACGTT 1 cut(s) 75
AclWI GGATC 6 cut(s) 82, 123, 310, 538, 766, 994
AcoI YGGCCR 2 cut(s) 157, 1080
AdeI CACNNNGTG 1 cut(s) 892
AfaI GTAC 3 cut(s) 163, 847, 1075
AfiI CCNNNNNNNGG 8 cut(s) 49, 97, 166, 205, 781, 889, 961, 1009
AgsI TTSAA 2 cut(s) 608, 959
AjnI CCWGG 4 cut(s) 90, 546, 774, 1002
AluBI AGCT 5 cut(s) 57, 148, 741, 969, 1060
AluI AGCT 5 cut(s) 57, 148, 741, 969, 1060
Alw21I GWGCWC 1 cut(s) 743
Alw26I GTCTC 1 cut(s) 1043
AlwI GGATC 6 cut(s) 82, 123, 310, 538, 766, 994
AlwNI CAGNNNCTG 1 cut(s) 355
Ama87I CYCGRG 1 cut(s) 44
Aor13HI TCCGGA 4 cut(s) 112, 340, 796, 1024
AoxI GGCC 6 cut(s) 81, 157, 309, 765, 993, 1080
ApeKI GCWGC 3 cut(s) 145, 1033, 1057
ArsI GACNNNNNNTTYG 2 cut(s) 709, 741
Asp700I GAANNNNTTC 1 cut(s) 1017
AspS9I GGNCC 6 cut(s) 208, 343, 571, 799, 1027, 1120
AsuHPI GGTGA 4 cut(s) 16, 31, 700, 943
AvaI CYCGRG 1 cut(s) 44
AvaII GGWCC 6 cut(s) 208, 343, 571, 799, 1027, 1120
AxyI CCTNAGG 1 cut(s) 212
BanII GRGCYC 1 cut(s) 743
BbsI GAAGAC 2 cut(s) 707, 1121
Bbv12I GWGCWC 1 cut(s) 743
BbvI GCAGC 3 cut(s) 157, 1045, 1069
BccI CCATC 8 cut(s) 74, 149, 302, 377, 605, 833, 956, 1061
BceAI ACGGC 1 cut(s) 144
BciT130I CCWGG 4 cut(s) 92, 548, 776, 1004
BciVI GTATCC 1 cut(s) 572
BcoDI GTCTC 1 cut(s) 1043
BfaI CTAG 1 cut(s) 149
BfuI GTATCC 1 cut(s) 572
BglII AGATCT 3 cut(s) 6, 690, 918
BisI GCNGC 3 cut(s) 146, 1034, 1058
BlsI GCNGC 3 cut(s) 147, 1035, 1059
Bme1390I CCNGG 4 cut(s) 92, 548, 776, 1004
Bme18I GGWCC 6 cut(s) 208, 343, 571, 799, 1027, 1120
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 6 cut(s) 208, 343, 571, 799, 1027, 1120
BmiI GGNNCC 2 cut(s) 218, 446
BmrFI CCNGG 4 cut(s) 92, 548, 776, 1004
BmrI ACTGGG 1 cut(s) 492
BmuI ACTGGG 1 cut(s) 492
BpiI GAAGAC 2 cut(s) 707, 1121
BpuEI CTTGAG 2 cut(s) 293, 1082
BsaJI CCNNGG 2 cut(s) 220, 448
BsaWI WCCGGW 8 cut(s) 26, 112, 340, 568, 710, 796, 938, 1024
Bsc4I CCNNNNNNNGG 8 cut(s) 49, 97, 166, 205, 781, 889, 961, 1009
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 1 cut(s) 487
Bse21I CCTNAGG 1 cut(s) 212
BseAI TCCGGA 4 cut(s) 112, 340, 796, 1024
BseBI CCWGG 4 cut(s) 92, 548, 776, 1004
BseDI CCNNGG 2 cut(s) 220, 448
BseGI GGATG 6 cut(s) 160, 388, 844, 864, 1072, 1092
BseLI CCNNNNNNNGG 8 cut(s) 49, 97, 166, 205, 781, 889, 961, 1009
BseMII CTCAG 2 cut(s) 226, 454
BseNI ACTGG 1 cut(s) 487
BseXI GCAGC 3 cut(s) 157, 1045, 1069
BshFI GGCC 6 cut(s) 83, 159, 311, 767, 995, 1082
BsiHKAI GWGCWC 1 cut(s) 743
BsiHKCI CYCGRG 1 cut(s) 44
BsiWI CGTACG 1 cut(s) 161
BslI CCNNNNNNNGG 8 cut(s) 49, 97, 166, 205, 781, 889, 961, 1009
BsmAI GTCTC 1 cut(s) 1043
BsmBI CGTCTC 1 cut(s) 1043
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 6 cut(s) 83, 159, 311, 767, 995, 1082
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 743
Bsp13I TCCGGA 4 cut(s) 112, 340, 796, 1024
BspACI CCGC 1 cut(s) 854
BspANI GGCC 6 cut(s) 83, 159, 311, 767, 995, 1082
BspCNI CTCAG 2 cut(s) 225, 453
BspEI TCCGGA 4 cut(s) 112, 340, 796, 1024
BspLI GGNNCC 2 cut(s) 218, 446
BspPI GGATC 6 cut(s) 82, 123, 310, 538, 766, 994
BsrFI RCCGGY 1 cut(s) 137
BsrI ACTGG 1 cut(s) 487
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 2 cut(s) 220, 448
Bst2UI CCWGG 4 cut(s) 92, 548, 776, 1004
Bst6I CTCTTC 1 cut(s) 1116
BstC8I GCNNGC 2 cut(s) 139, 143
BstDEI CTNAG 2 cut(s) 212, 440
BstDSI CCRYGG 2 cut(s) 220, 448
BstF5I GGATG 6 cut(s) 160, 388, 844, 864, 1072, 1092
BstMAI GTCTC 1 cut(s) 1043
BstNI CCWGG 4 cut(s) 92, 548, 776, 1004
BstSCI CCNGG 4 cut(s) 90, 546, 774, 1002
BstV1I GCAGC 3 cut(s) 157, 1045, 1069
BstV2I GAAGAC 2 cut(s) 707, 1121
BstX2I RGATCY 8 cut(s) 6, 87, 315, 543, 690, 771, 918, 999
BstYI RGATCY 8 cut(s) 6, 87, 315, 543, 690, 771, 918, 999
Bsu36I CCTNAGG 1 cut(s) 212
BsuI GTATCC 1 cut(s) 572
BsuRI GGCC 6 cut(s) 83, 159, 311, 767, 995, 1082
BtgI CCRYGG 2 cut(s) 220, 448
BtsCI GGATG 6 cut(s) 160, 388, 844, 864, 1072, 1092
Cac8I GCNNGC 2 cut(s) 139, 143
CaiI CAGNNNCTG 1 cut(s) 355
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 6 cut(s) 208, 343, 571, 799, 1027, 1120
CseI GACGC 4 cut(s) 659, 887, 1025, 1115
Csp6I GTAC 3 cut(s) 162, 846, 1074
CviQI GTAC 3 cut(s) 162, 846, 1074
DdeI CTNAG 2 cut(s) 212, 440
DraIII CACNNNGTG 1 cut(s) 892
EaeI YGGCCR 2 cut(s) 157, 1080
Eam1104I CTCTTC 1 cut(s) 1116
EarI CTCTTC 1 cut(s) 1116
EciI GGCGGA 1 cut(s) 869
Ecl136II GAGCTC 1 cut(s) 741
Eco24I GRGCYC 1 cut(s) 743
Eco47I GGWCC 6 cut(s) 208, 343, 571, 799, 1027, 1120
Eco53kI GAGCTC 1 cut(s) 741
Eco81I CCTNAGG 1 cut(s) 212
Eco88I CYCGRG 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 741
EcoRII CCWGG 4 cut(s) 90, 546, 774, 1002
EcoT38I GRGCYC 1 cut(s) 743
Esp3I CGTCTC 1 cut(s) 1043
FaiI YATR 4 cut(s) 230, 458, 686, 914
FblI GTMKAC 2 cut(s) 878, 1106
Fnu4HI GCNGC 3 cut(s) 146, 1034, 1058
FokI GGATG 6 cut(s) 167, 395, 851, 851, 1079, 1079
FriOI GRGCYC 1 cut(s) 743
Fsp4HI GCNGC 3 cut(s) 146, 1034, 1058
FspBI CTAG 1 cut(s) 149
GluI GCNGC 3 cut(s) 146, 1034, 1058
HaeIII GGCC 6 cut(s) 83, 159, 311, 767, 995, 1082
HgaI GACGC 4 cut(s) 659, 887, 1025, 1115
HincII GTYRAC 2 cut(s) 480, 879
HindII GTYRAC 2 cut(s) 480, 879
HinfI GANTC 8 cut(s) 19, 191, 419, 647, 703, 789, 875, 1103
HphI GGTGA 4 cut(s) 16, 31, 700, 943
Hpy166II GTNNAC 5 cut(s) 423, 480, 651, 879, 1107
Hpy188I TCNGA 6 cut(s) 61, 289, 517, 642, 745, 973
Hpy8I GTNNAC 5 cut(s) 423, 480, 651, 879, 1107
HpyCH4IV ACGT 8 cut(s) 75, 164, 303, 476, 531, 759, 987, 1072
HpyCH4V TGCA 5 cut(s) 4, 232, 460, 688, 916
HpyF3I CTNAG 2 cut(s) 212, 440
HpySE526I ACGT 8 cut(s) 75, 164, 303, 476, 531, 759, 987, 1072
Kpn2I TCCGGA 4 cut(s) 112, 340, 796, 1024
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
LmnI GCTCC 4 cut(s) 62, 222, 450, 974
Lsp1109I GCAGC 3 cut(s) 157, 1045, 1069
MaeI CTAG 1 cut(s) 149
MaeII ACGT 8 cut(s) 75, 164, 303, 476, 531, 759, 987, 1072
MfeI CAATTG 1 cut(s) 830
MflI RGATCY 8 cut(s) 6, 87, 315, 543, 690, 771, 918, 999
MhlI GDGCHC 1 cut(s) 743
MluCI AATT 1 cut(s) 830
MlyI GAGTC 6 cut(s) 13, 428, 656, 697, 884, 1112
MmeI TCCRAC 3 cut(s) 84, 357, 996
MroI TCCGGA 4 cut(s) 112, 340, 796, 1024
MroNI GCCGGC 1 cut(s) 137
MroXI GAANNNNTTC 1 cut(s) 1017
MseI TTAA 4 cut(s) 15, 78, 243, 471
MspR9I CCNGG 4 cut(s) 92, 548, 776, 1004
MunI CAATTG 1 cut(s) 830
Mva1269I GAATGC 1 cut(s) 105
MvaI CCWGG 4 cut(s) 92, 548, 776, 1004
NaeI GCCGGC 1 cut(s) 139
NgoMIV GCCGGC 1 cut(s) 137
NlaIV GGNNCC 2 cut(s) 218, 446
PaeR7I CTCGAG 1 cut(s) 44
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PctI GAATGC 1 cut(s) 105
PdiI GCCGGC 1 cut(s) 139
PdmI GAANNNNTTC 1 cut(s) 1017
PfeI GAWTC 2 cut(s) 191, 789
Pfl23II CGTACG 1 cut(s) 161
PflFI GACNNNGTC 2 cut(s) 302, 986
PfoI TCCNGGA 4 cut(s) 90, 546, 774, 1002
PkrI GCNGC 3 cut(s) 147, 1035, 1059
PleI GAGTC 6 cut(s) 13, 427, 655, 697, 883, 1111
PpsI GAGTC 6 cut(s) 13, 427, 655, 697, 883, 1111
Psp124BI GAGCTC 1 cut(s) 743
Psp1406I AACGTT 1 cut(s) 75
Psp6I CCWGG 4 cut(s) 90, 546, 774, 1002
PspGI CCWGG 4 cut(s) 90, 546, 774, 1002
PspLI CGTACG 1 cut(s) 161
PspN4I GGNNCC 2 cut(s) 218, 446
PspPI GGNCC 6 cut(s) 208, 343, 571, 799, 1027, 1120
PspXI VCTCGAGB 1 cut(s) 44
PstNI CAGNNNCTG 1 cut(s) 355
PsuI RGATCY 8 cut(s) 6, 87, 315, 543, 690, 771, 918, 999
PsyI GACNNNGTC 2 cut(s) 302, 986
RsaI GTAC 3 cut(s) 163, 847, 1075
RsaNI GTAC 3 cut(s) 162, 846, 1074
SacI GAGCTC 1 cut(s) 743
SalI GTCGAC 1 cut(s) 877
SaqAI TTAA 4 cut(s) 15, 78, 243, 471
SatI GCNGC 3 cut(s) 146, 1034, 1058
Sau96I GGNCC 6 cut(s) 208, 343, 571, 799, 1027, 1120
SchI GAGTC 6 cut(s) 13, 428, 656, 697, 884, 1112
ScrFI CCNGG 4 cut(s) 92, 548, 776, 1004
SduI GDGCHC 1 cut(s) 743
Sfr274I CTCGAG 1 cut(s) 44
SinI GGWCC 6 cut(s) 208, 343, 571, 799, 1027, 1120
SlaI CTCGAG 1 cut(s) 44
SmlI CTYRAG 3 cut(s) 44, 272, 1061
SmoI CTYRAG 3 cut(s) 44, 272, 1061
Sse9I AATT 1 cut(s) 830
SsiI CCGC 1 cut(s) 854
SspMI CTAG 1 cut(s) 149
SstI GAGCTC 1 cut(s) 743
StyD4I CCNGG 4 cut(s) 90, 546, 774, 1002
TaiI ACGT 8 cut(s) 78, 167, 306, 479, 534, 762, 990, 1075
TaqI TCGA 4 cut(s) 45, 69, 297, 878
TasI AATT 1 cut(s) 830
TfiI GAWTC 2 cut(s) 191, 789
Tru1I TTAA 4 cut(s) 15, 78, 243, 471
Tru9I TTAA 4 cut(s) 15, 78, 243, 471
TseI GCWGC 3 cut(s) 145, 1033, 1057
TspDTI ATGAA 1 cut(s) 419
TspGWI ACGGA 2 cut(s) 209, 437
Tth111I GACNNNGTC 2 cut(s) 302, 986
VpaK11BI GGWCC 6 cut(s) 208, 343, 571, 799, 1027, 1120
XcmI CCANNNNNNNNNTGG 2 cut(s) 202, 658
XhoI CTCGAG 1 cut(s) 44
XmiI GTMKAC 2 cut(s) 878, 1106
XmnI GAANNNNTTC 1 cut(s) 1017
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.