Rh5AG255500

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0017475
Physical Location & Seq
Forward (+)
33239460 .. 33241869
2410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG255500.1

Sequence Viewer

Length: 1146 bp
ATGCAGATCTTTGTGAAAACCCTCACAGGCAAGACTATTACCCTCGAGGTTGAAAGCTCCGACACCATCGACAATGTCAAAGCGAAGATCCAAGACAAGGAGGGAATTCCCCCAGACCAGCAGAGGCTGATCTTTGCTGGCAAGCAGCTTGAGGACGGCCGGACACTTGCTGATTATAACATTCAGAAGGAGTCCACTCTCCACTTGGTGCTCAGACTCCGTGGTGGCATGCAGATCTTTGTCAAGACTCTCACAGGGAAGACCATCACCTTGGAGGTCGAGAGCTCTGATACAATCGATAATGTCAAGGCAAAGATCCAGGACAAGGAGGGTATCCCCCCAGATCAGCAGAGGCTCATCTTTGCCGGCAAACAGCTAGAGGATGGTCGCACCCTTGCAGATTACAATATCCAAAAGGAGTCCACCCTTCACTTGGTGCTTCGTCTCCGTGGGGGAATGCAAATCTTTGTGAAGACCCTTACTGGGAAGACAATCACCTTGGAGGTGGAAAGCTCTGATACAATTGACAATGTCAAGGCAAAGATCCAGGACAAGGAGGGTATTCCCCCTGATCAGCAGAGGCTCATCTTTGCAGGTAAACAGTTGGAGGACGGAAGAACTCTTGCAGACTATAACATTCAAAAGGAGTCGACCTTGCACTTGGTTCTCCGGCTGCGTGGTGGAATGCAAATTTTTGTGAAGACTTTGACAGGGAAAACAATTACTTTGGAAGTGGAAAGCTCGGACACAATTGATAATGTCAAGGCGAAGATTCAGGACAAAGAAGGCATTCCCCCAGACCAGCAGAGGCTCATCTTTGCAGGAAAGCAGCTTGAAGACGGTAGGACACTGGCAGACTACAACATCCAGAAGGAGTCGACTCTGCATTTGGTGCTCCGTCTACGTGGTGGGATGCAGATTTTTGTGAAGACCCTCACTGGAAAGACCATAACCTTGGAGGTAGAGAGTTCTGATACAATTGATAATGTGAAAGCAAAGATTCAGGACAAGGAGGGCATTCCTCCGGACCAGCAGAGGCTCATATTTGCTGGTAAGCAGCTTGAAGATGGGAGGACTTTAGCGGATTACAACATCCAGAAGGAGTCGACCCTTCACCTTGTCCTGCGCCTCCGTGGTGGTTTCTGA

Protein Analysis

381

Amino Acids

42.7

Weight (kDa)

7.0

Isoelectric Point (pI)

29.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 74 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 77 - 147 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 79 - 150 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 153 - 223 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 155 - 226 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 229 - 299 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 231 - 302 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 305 - 375 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 307 - 378 1.1e-31 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017475)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05320 AT4G05320 AT4G05320
fragaria_vesca FvH4_3g21751 FvH4_3g21751 FvH4_3g21853
malus_domestica MD03G1211300.v1.1
prunus_persica Prupe.4G025900_v2.0.a1 Prupe.4G025900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0003941
rosa_samantha Rh5AG255500
rosa_wichuraiana Rw3G014130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AbsI CCTCGAGG 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 584
AccI GTMKAC 4 cut(s) 650, 878, 901, 1106
AccIII TCCGGA 1 cut(s) 1024
AciI CCGC 1 cut(s) 1082
AclWI GGATC 3 cut(s) 82, 310, 538
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 2 cut(s) 105, 690
AdeI CACNNNGTG 2 cut(s) 208, 436
AfiI CCNNNNNNNGG 5 cut(s) 97, 325, 433, 483, 553
AgsI TTSAA 4 cut(s) 53, 641, 836, 1064
AjnI CCWGG 2 cut(s) 318, 546
AluBI AGCT 8 cut(s) 57, 148, 285, 376, 513, 741, 832, 1060
AluI AGCT 8 cut(s) 57, 148, 285, 376, 513, 741, 832, 1060
Alw21I GWGCWC 3 cut(s) 213, 287, 897
Alw26I GTCTC 1 cut(s) 449
AlwI GGATC 3 cut(s) 82, 310, 538
AlwNI CAGNNNCTG 1 cut(s) 127
Ama87I CYCGRG 1 cut(s) 44
Aor13HI TCCGGA 1 cut(s) 1024
AoxI GGCC 1 cut(s) 157
ApeKI GCWGC 4 cut(s) 145, 673, 829, 1057
ApoI RAATTY 2 cut(s) 105, 690
ArsI GACNNNNNNTTYG 2 cut(s) 871, 903
Asp700I GAANNNNTTC 1 cut(s) 789
AspLEI GCGC 1 cut(s) 1128
AspS9I GGNCC 1 cut(s) 1027
AsuHPI GGTGA 3 cut(s) 259, 487, 1106
AvaI CYCGRG 1 cut(s) 44
AvaII GGWCC 1 cut(s) 1027
BanII GRGCYC 1 cut(s) 287
BbsI GAAGAC 6 cut(s) 266, 479, 494, 707, 843, 935
Bbv12I GWGCWC 3 cut(s) 213, 287, 897
BbvI GCAGC 4 cut(s) 157, 660, 841, 1069
BccI CCATC 4 cut(s) 74, 272, 377, 1061
BceAI ACGGC 1 cut(s) 172
BciT130I CCWGG 2 cut(s) 320, 548
BciVI GTATCC 1 cut(s) 344
BclI TGATCA 1 cut(s) 571
BcoDI GTCTC 1 cut(s) 449
BfaI CTAG 1 cut(s) 377
BfuAI ACCTGC 1 cut(s) 584
BfuI GTATCC 1 cut(s) 344
BglII AGATCT 2 cut(s) 6, 234
BisI GCNGC 4 cut(s) 146, 674, 830, 1058
BlsI GCNGC 4 cut(s) 147, 675, 831, 1059
Bme1390I CCNGG 2 cut(s) 320, 548
Bme18I GGWCC 1 cut(s) 1027
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 1027
BmrFI CCNGG 2 cut(s) 320, 548
BmrI ACTGGG 1 cut(s) 492
BmsI GCATC 1 cut(s) 903
BmuI ACTGGG 1 cut(s) 492
BpiI GAAGAC 6 cut(s) 266, 479, 494, 707, 843, 935
BpuEI CTTGAG 1 cut(s) 170
Bsa29I ATCGAT 1 cut(s) 297
BsaAI YACGTR 1 cut(s) 905
BsaJI CCNNGG 6 cut(s) 220, 270, 448, 498, 954, 1132
BsaWI WCCGGW 1 cut(s) 1024
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 325, 433, 483, 553
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 3 cut(s) 487, 855, 943
BseAI TCCGGA 1 cut(s) 1024
BseBI CCWGG 2 cut(s) 320, 548
BseCI ATCGAT 1 cut(s) 297
BseDI CCNNGG 6 cut(s) 220, 270, 448, 498, 954, 1132
BseGI GGATG 4 cut(s) 388, 864, 918, 1092
BseLI CCNNNNNNNGG 5 cut(s) 97, 325, 433, 483, 553
BseMII CTCAG 1 cut(s) 226
BseNI ACTGG 3 cut(s) 487, 855, 943
BseX3I CGGCCG 1 cut(s) 157
BseXI GCAGC 4 cut(s) 157, 660, 841, 1069
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 1 cut(s) 159
BshVI ATCGAT 1 cut(s) 297
BsiEI CGRYCG 1 cut(s) 160
BsiHKAI GWGCWC 3 cut(s) 213, 287, 897
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 4 cut(s) 160, 366, 670, 1025
BslI CCNNNNNNNGG 5 cut(s) 97, 325, 433, 483, 553
BsmAI GTCTC 1 cut(s) 449
BsmBI CGTCTC 1 cut(s) 449
BsmI GAATGC 4 cut(s) 462, 690, 789, 1017
BsnI GGCC 1 cut(s) 159
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 3 cut(s) 213, 287, 897
Bsp13I TCCGGA 1 cut(s) 1024
Bsp143I GATC 8 cut(s) 6, 87, 129, 234, 315, 343, 543, 571
BspACI CCGC 1 cut(s) 1082
BspANI GGCC 1 cut(s) 159
BspCNI CTCAG 1 cut(s) 225
BspDI ATCGAT 1 cut(s) 297
BspEI TCCGGA 1 cut(s) 1024
BspMI ACCTGC 1 cut(s) 584
BspPI GGATC 3 cut(s) 82, 310, 538
BsrFI RCCGGY 1 cut(s) 365
BsrI ACTGG 3 cut(s) 487, 855, 943
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 6 cut(s) 220, 270, 448, 498, 954, 1132
BssMI GATC 8 cut(s) 6, 87, 129, 234, 315, 343, 543, 571
BssT1I CCWWGG 3 cut(s) 270, 498, 954
Bst2UI CCWGG 2 cut(s) 320, 548
Bst4CI ACNGT 2 cut(s) 603, 842
BstAPI GCANNNNNTGC 1 cut(s) 892
BstBAI YACGTR 1 cut(s) 905
BstC8I GCNNGC 4 cut(s) 139, 143, 230, 367
BstDEI CTNAG 1 cut(s) 212
BstDSI CCRYGG 3 cut(s) 220, 448, 1132
BstF5I GGATG 4 cut(s) 388, 864, 918, 1092
BstHHI GCGC 1 cut(s) 1128
BstKTI GATC 8 cut(s) 9, 90, 132, 237, 318, 346, 546, 574
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 8 cut(s) 6, 87, 129, 234, 315, 343, 543, 571
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 1 cut(s) 892
BstNI CCWGG 2 cut(s) 320, 548
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 2 cut(s) 318, 546
BstV1I GCAGC 4 cut(s) 157, 660, 841, 1069
BstV2I GAAGAC 6 cut(s) 266, 479, 494, 707, 843, 935
BstX2I RGATCY 5 cut(s) 6, 87, 234, 315, 543
BstXI CCANNNNNNTGG 2 cut(s) 271, 955
BstYI RGATCY 5 cut(s) 6, 87, 234, 315, 543
BstZI CGGCCG 1 cut(s) 157
Bsu15I ATCGAT 1 cut(s) 297
BsuI GTATCC 1 cut(s) 344
BsuRI GGCC 1 cut(s) 159
BsuTUI ATCGAT 1 cut(s) 297
BtgI CCRYGG 3 cut(s) 220, 448, 1132
BtsCI GGATG 4 cut(s) 388, 864, 918, 1092
BtsIMutI CAGTG 2 cut(s) 848, 936
BveI ACCTGC 1 cut(s) 584
Cac8I GCNNGC 4 cut(s) 139, 143, 230, 367
CaiI CAGNNNCTG 1 cut(s) 127
CfoI GCGC 1 cut(s) 1128
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 1 cut(s) 1027
ClaI ATCGAT 1 cut(s) 297
CviAII CATG 1 cut(s) 229
DdeI CTNAG 1 cut(s) 212
DpnI GATC 8 cut(s) 8, 89, 131, 236, 317, 345, 545, 573
DpnII GATC 8 cut(s) 6, 87, 129, 234, 315, 343, 543, 571
DraIII CACNNNGTG 2 cut(s) 208, 436
EaeI YGGCCR 1 cut(s) 157
EagI CGGCCG 1 cut(s) 157
Ecl136II GAGCTC 1 cut(s) 285
EclXI CGGCCG 1 cut(s) 157
Eco130I CCWWGG 3 cut(s) 270, 498, 954
Eco24I GRGCYC 1 cut(s) 287
Eco47I GGWCC 1 cut(s) 1027
Eco52I CGGCCG 1 cut(s) 157
Eco53kI GAGCTC 1 cut(s) 285
Eco88I CYCGRG 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 285
EcoRI GAATTC 1 cut(s) 105
EcoRII CCWGG 2 cut(s) 318, 546
EcoT14I CCWWGG 3 cut(s) 270, 498, 954
EcoT38I GRGCYC 1 cut(s) 287
ErhI CCWWGG 3 cut(s) 270, 498, 954
Esp3I CGTCTC 1 cut(s) 449
FaeI CATG 1 cut(s) 232
FaiI YATR 5 cut(s) 177, 230, 633, 950, 1043
FatI CATG 1 cut(s) 228
FbaI TGATCA 1 cut(s) 571
FblI GTMKAC 4 cut(s) 650, 878, 901, 1106
Fnu4HI GCNGC 4 cut(s) 146, 674, 830, 1058
FokI GGATG 4 cut(s) 395, 851, 925, 1079
FriOI GRGCYC 1 cut(s) 287
Fsp4HI GCNGC 4 cut(s) 146, 674, 830, 1058
FspBI CTAG 1 cut(s) 377
GlaI GCGC 1 cut(s) 1127
GluI GCNGC 4 cut(s) 146, 674, 830, 1058
HaeIII GGCC 1 cut(s) 159
HapII CCGG 4 cut(s) 160, 366, 670, 1025
HhaI GCGC 1 cut(s) 1128
Hin1II CATG 1 cut(s) 232
Hin6I GCGC 1 cut(s) 1126
HinP1I GCGC 1 cut(s) 1126
HincII GTYRAC 3 cut(s) 651, 879, 1107
HindII GTYRAC 3 cut(s) 651, 879, 1107
HpaII CCGG 4 cut(s) 160, 366, 670, 1025
HphI GGTGA 3 cut(s) 259, 487, 1106
Hpy166II GTNNAC 7 cut(s) 195, 423, 599, 651, 879, 902, 1107
Hpy188I TCNGA 8 cut(s) 61, 186, 215, 289, 517, 745, 973, 1145
Hpy188III TCNNGA 7 cut(s) 244, 280, 776, 868, 1004, 1025, 1096
Hpy8I GTNNAC 7 cut(s) 195, 423, 599, 651, 879, 902, 1107
HpyAV CCTTC 6 cut(s) 181, 437, 779, 865, 1093, 1121
HpyCH4III ACNGT 2 cut(s) 603, 842
HpyCH4IV ACGT 1 cut(s) 904
HpyF10VI GCNNNNNNNGC 1 cut(s) 892
HpyF3I CTNAG 1 cut(s) 212
HpySE526I ACGT 1 cut(s) 904
Hsp92II CATG 1 cut(s) 232
HspAI GCGC 1 cut(s) 1126
Kpn2I TCCGGA 1 cut(s) 1024
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
Ksp22I TGATCA 1 cut(s) 571
Kzo9I GATC 8 cut(s) 6, 87, 129, 234, 315, 343, 543, 571
LmnI GCTCC 2 cut(s) 62, 900
Lsp1109I GCAGC 4 cut(s) 157, 660, 841, 1069
LweI GCATC 1 cut(s) 903
MaeI CTAG 1 cut(s) 377
MaeII ACGT 1 cut(s) 904
MalI GATC 8 cut(s) 8, 89, 131, 236, 317, 345, 545, 573
MboI GATC 8 cut(s) 6, 87, 129, 234, 315, 343, 543, 571
MfeI CAATTG 3 cut(s) 522, 750, 978
MflI RGATCY 5 cut(s) 6, 87, 234, 315, 543
MhlI GDGCHC 3 cut(s) 213, 287, 897
MluCI AATT 6 cut(s) 105, 522, 690, 720, 750, 978
MlyI GAGTC 8 cut(s) 200, 210, 241, 428, 656, 874, 884, 1112
MmeI TCCRAC 2 cut(s) 84, 585
MroI TCCGGA 1 cut(s) 1024
MroNI GCCGGC 1 cut(s) 365
MroXI GAANNNNTTC 1 cut(s) 789
MspI CCGG 4 cut(s) 160, 366, 670, 1025
MspR9I CCNGG 2 cut(s) 320, 548
MunI CAATTG 3 cut(s) 522, 750, 978
Mva1269I GAATGC 4 cut(s) 462, 690, 789, 1017
MvaI CCWGG 2 cut(s) 320, 548
MwoI GCNNNNNNNGC 1 cut(s) 892
NaeI GCCGGC 1 cut(s) 367
NdeII GATC 8 cut(s) 6, 87, 129, 234, 315, 343, 543, 571
NgoMIV GCCGGC 1 cut(s) 365
NlaIII CATG 1 cut(s) 232
NspI RCATGY 1 cut(s) 232
PaeI GCATGC 1 cut(s) 232
PaeR7I CTCGAG 1 cut(s) 44
PctI GAATGC 4 cut(s) 462, 690, 789, 1017
PdiI GCCGGC 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 789
PfeI GAWTC 2 cut(s) 772, 1000
PflFI GACNNNGTC 2 cut(s) 74, 530
PfoI TCCNGGA 2 cut(s) 318, 546
PkrI GCNGC 4 cut(s) 147, 675, 831, 1059
PleI GAGTC 8 cut(s) 199, 210, 241, 427, 655, 874, 883, 1111
PpsI GAGTC 8 cut(s) 199, 210, 241, 427, 655, 874, 883, 1111
Ppu21I YACGTR 1 cut(s) 905
PsiI TTATAA 1 cut(s) 177
Psp124BI GAGCTC 1 cut(s) 287
Psp6I CCWGG 2 cut(s) 318, 546
PspGI CCWGG 2 cut(s) 318, 546
PspPI GGNCC 1 cut(s) 1027
PspXI VCTCGAGB 1 cut(s) 44
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 5 cut(s) 6, 87, 234, 315, 543
PsyI GACNNNGTC 2 cut(s) 74, 530
SacI GAGCTC 1 cut(s) 287
SalI GTCGAC 3 cut(s) 649, 877, 1105
SatI GCNGC 4 cut(s) 146, 674, 830, 1058
Sau3AI GATC 8 cut(s) 6, 87, 129, 234, 315, 343, 543, 571
Sau96I GGNCC 1 cut(s) 1027
SchI GAGTC 8 cut(s) 200, 210, 241, 428, 656, 874, 884, 1112
ScrFI CCNGG 2 cut(s) 320, 548
SduI GDGCHC 3 cut(s) 213, 287, 897
SfaNI GCATC 1 cut(s) 903
Sfr274I CTCGAG 1 cut(s) 44
SinI GGWCC 1 cut(s) 1027
SlaI CTCGAG 1 cut(s) 44
SmlI CTYRAG 2 cut(s) 44, 149
SmoI CTYRAG 2 cut(s) 44, 149
SphI GCATGC 1 cut(s) 232
Sse9I AATT 6 cut(s) 105, 522, 690, 720, 750, 978
SsiI CCGC 1 cut(s) 1082
SspMI CTAG 1 cut(s) 377
SstI GAGCTC 1 cut(s) 287
StyD4I CCNGG 2 cut(s) 318, 546
StyI CCWWGG 3 cut(s) 270, 498, 954
TaaI ACNGT 2 cut(s) 603, 842
TaiI ACGT 1 cut(s) 907
TaqI TCGA 7 cut(s) 45, 69, 279, 297, 650, 878, 1106
TasI AATT 6 cut(s) 105, 522, 690, 720, 750, 978
TfiI GAWTC 2 cut(s) 772, 1000
TscAI CASTG 2 cut(s) 855, 943
TseI GCWGC 4 cut(s) 145, 673, 829, 1057
TspGWI ACGGA 5 cut(s) 209, 437, 627, 887, 1121
TspRI CASTG 2 cut(s) 855, 943
Tth111I GACNNNGTC 2 cut(s) 74, 530
VpaK11BI GGWCC 1 cut(s) 1027
XapI RAATTY 2 cut(s) 105, 690
XceI RCATGY 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 2 cut(s) 202, 430
XhoI CTCGAG 1 cut(s) 44
XmiI GTMKAC 4 cut(s) 650, 878, 901, 1106
XmnI GAANNNNTTC 1 cut(s) 789
XspI CTAG 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.