FvH4_3g21751

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011635
Physical Location & Seq
Forward (+)
14795380 .. 14798458
3079 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21751.t1

Sequence Viewer

Length: 1146 bp
ATGCAGATCTTCGTTAAGACTCTCACTGGCAAGACCATCACCCTTGAGGTTGAAAGCTCTGACACCATCGACAATGTCAAAGCCAAAATCCAAGATAAGGAAGGCATTCCCCCAGACCAGCAAAGACTGATTTTTGCTGGAAAGCAGCTCGAGGATGGACGCACCCTTGCTGACTACAATATTCAAAAGGAATCGACTCTACACCTGGTGCTCAGACTCCGCGGTGGCATGCAAATCTTTGTGAAGACTCTCACTGGGAAAACCATAACCTTGGAGGTTGAGAGTTCTGACACCATTGACAACGTGAAGGCAAAGATTCAGGATAAAGAGGGTATCCCACCAGACCAGCAGAGACTCATCTTTGCTGGGAAGCAGCTTGAGGATGGCCGCACCCTGGCTGATTACAACATTCAAAAGGAGTCCACCCTTCATTTAGTGCTTCGTCTACGTGGTGGCATGCAAATCTTTGTCAAGACCCTCACCGGAAAGACAATCACCTTGGAGGTTGAAAGTTCAGATACAATTGATAATGTCAAGGCAAAGATTCAGGACAAAGAGGGCATTCCTCCAGACCAGCAGAGGCTCATTTTTGCAGGGAAACAGCTAGAGGATGGTCGCACTCTTGCGGACTACAATATTCAGAAGGAATCAACTCTTCACTTGGTGTTGCGTCTCCGTGGTGGCATGCAAATCTTTGTCAAGACCCTTACCGGCAAAACCATCACTTTGGAAGTGGAAAGCTCTGACACCATAGATAATGTGAAAGCTAAAATTCAGGATAAGGAGGGTATTCCTCCAGACCAGCAGAGGTTGATTTTTGCAGGTAAGCAGCTTGAGGATGGAAGGACACTTGCAGACTATAATATTCAAAAGGAGTCCACTCTGCACTTGGTGCTTCGTCTGCGCGGTGGTATGCAGATCTTTGTCAAAACTCTGACAGGGAAGACCATAACATTGGAGGTAGAGAGTTCAGATACGATTGACAATGTGAAAGCCAAGATTCAGGATAAGGAGGGCATCCCCCCAGACCAGCAGAGGCTCATATTCGCTGGAAAGCAGCTTGAGGATGGGAGGACTCTTGCAGATTACAACATTCAGAAGGAGTCCACCCTTCACCTTGTCCTTCGCCTCCGTGGTGGTTTCTGA

Protein Analysis

382

Amino Acids

42.7

Weight (kDa)

7.0

Isoelectric Point (pI)

29.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 74 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 77 - 147 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 79 - 150 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 153 - 223 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 155 - 226 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 229 - 299 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 231 - 302 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 305 - 375 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 307 - 378 1.1e-31 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 812
AccI GTMKAC 1 cut(s) 445
AccII CGCG 2 cut(s) 222, 906
AciI CCGC 5 cut(s) 220, 222, 388, 626, 906
AcoI YGGCCR 1 cut(s) 385
AcsI RAATTY 1 cut(s) 771
AdeI CACNNNGTG 3 cut(s) 208, 664, 892
AfiI CCNNNNNNNGG 2 cut(s) 97, 394
AgsI TTSAA 5 cut(s) 53, 185, 413, 509, 869
AjnI CCWGG 2 cut(s) 204, 393
AluBI AGCT 8 cut(s) 57, 148, 376, 604, 741, 767, 832, 1060
AluI AGCT 8 cut(s) 57, 148, 376, 604, 741, 767, 832, 1060
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 2 cut(s) 346, 677
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 1 cut(s) 385
ApeKI GCWGC 4 cut(s) 145, 373, 829, 1057
ApoI RAATTY 1 cut(s) 771
Asp700I GAANNNNTTC 1 cut(s) 105
AspLEI GCGC 1 cut(s) 906
AsuHPI GGTGA 4 cut(s) 31, 472, 487, 1106
AvaI CYCGRG 1 cut(s) 149
BbsI GAAGAC 2 cut(s) 251, 950
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 4 cut(s) 157, 385, 841, 1069
BccI CCATC 8 cut(s) 44, 74, 149, 377, 605, 728, 833, 1061
BciT130I CCWGG 2 cut(s) 206, 395
BciVI GTATCC 1 cut(s) 344
BcoDI GTCTC 2 cut(s) 346, 677
BfaI CTAG 1 cut(s) 605
BfuAI ACCTGC 1 cut(s) 812
BfuI GTATCC 1 cut(s) 344
BglII AGATCT 2 cut(s) 6, 918
BisI GCNGC 5 cut(s) 146, 374, 388, 830, 1058
BlsI GCNGC 5 cut(s) 147, 375, 389, 831, 1059
Bme1390I CCNGG 2 cut(s) 206, 395
BmeT110I CYCGRG 1 cut(s) 149
BmrFI CCNGG 2 cut(s) 206, 395
BmrI ACTGGG 1 cut(s) 264
BmsI GCATC 1 cut(s) 1026
BmuI ACTGGG 1 cut(s) 264
BpiI GAAGAC 2 cut(s) 251, 950
BpmI CTGGAG 2 cut(s) 552, 780
BpuEI CTTGAG 4 cut(s) 65, 398, 854, 1082
BsaAI YACGTR 1 cut(s) 449
BsaJI CCNNGG 6 cut(s) 220, 270, 393, 498, 676, 1132
BsaWI WCCGGW 1 cut(s) 482
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 394
Bse118I RCCGGY 1 cut(s) 710
Bse1I ACTGG 2 cut(s) 31, 259
BseBI CCWGG 2 cut(s) 206, 395
BseDI CCNNGG 6 cut(s) 220, 270, 393, 498, 676, 1132
BseGI GGATG 6 cut(s) 160, 388, 616, 844, 1017, 1072
BseLI CCNNNNNNNGG 2 cut(s) 97, 394
BseMII CTCAG 1 cut(s) 226
BseNI ACTGG 2 cut(s) 31, 259
BseXI GCAGC 4 cut(s) 157, 385, 841, 1069
BseYI CCCAGC 1 cut(s) 365
BsgI GTGCAG 1 cut(s) 869
Bsh1236I CGCG 2 cut(s) 222, 906
BshFI GGCC 1 cut(s) 387
BsiHKAI GWGCWC 1 cut(s) 213
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 2 cut(s) 483, 711
BslI CCNNNNNNNGG 2 cut(s) 97, 394
BsmAI GTCTC 2 cut(s) 346, 677
BsmBI CGTCTC 1 cut(s) 677
BsmI GAATGC 2 cut(s) 105, 561
BsnI GGCC 1 cut(s) 387
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 2 cut(s) 6, 918
BspACI CCGC 5 cut(s) 220, 222, 388, 626, 906
BspANI GGCC 1 cut(s) 387
BspCNI CTCAG 1 cut(s) 225
BspFNI CGCG 2 cut(s) 222, 906
BspMI ACCTGC 1 cut(s) 812
BsrFI RCCGGY 1 cut(s) 710
BsrI ACTGG 2 cut(s) 31, 259
BssAI RCCGGY 1 cut(s) 710
BssECI CCNNGG 6 cut(s) 220, 270, 393, 498, 676, 1132
BssMI GATC 2 cut(s) 6, 918
BssT1I CCWWGG 2 cut(s) 270, 498
Bst2UI CCWGG 2 cut(s) 206, 395
Bst6I CTCTTC 1 cut(s) 660
BstAPI GCANNNNNTGC 1 cut(s) 892
BstBAI YACGTR 1 cut(s) 449
BstC8I GCNNGC 3 cut(s) 230, 458, 686
BstDEI CTNAG 1 cut(s) 212
BstDSI CCRYGG 3 cut(s) 220, 676, 1132
BstF5I GGATG 6 cut(s) 160, 388, 616, 844, 1017, 1072
BstFNI CGCG 2 cut(s) 222, 906
BstHHI GCGC 1 cut(s) 906
BstKTI GATC 2 cut(s) 9, 921
BstMAI GTCTC 2 cut(s) 346, 677
BstMBI GATC 2 cut(s) 6, 918
BstMWI GCNNNNNNNGC 2 cut(s) 892, 901
BstNI CCWGG 2 cut(s) 206, 395
BstNSI RCATGY 3 cut(s) 232, 460, 688
BstSCI CCNGG 2 cut(s) 204, 393
BstUI CGCG 2 cut(s) 222, 906
BstV1I GCAGC 4 cut(s) 157, 385, 841, 1069
BstV2I GAAGAC 2 cut(s) 251, 950
BstX2I RGATCY 2 cut(s) 6, 918
BstXI CCANNNNNNTGG 3 cut(s) 271, 727, 955
BstYI RGATCY 2 cut(s) 6, 918
BsuI GTATCC 1 cut(s) 344
BsuRI GGCC 1 cut(s) 387
BtgI CCRYGG 3 cut(s) 220, 676, 1132
BtsCI GGATG 6 cut(s) 160, 388, 616, 844, 1017, 1072
BtsIMutI CAGTG 2 cut(s) 24, 252
BveI ACCTGC 1 cut(s) 812
Cac8I GCNNGC 3 cut(s) 230, 458, 686
CfoI GCGC 1 cut(s) 906
Cfr10I RCCGGY 1 cut(s) 710
Cfr42I CCGCGG 1 cut(s) 223
CseI GACGC 2 cut(s) 168, 659
CsiI ACCWGGT 1 cut(s) 204
CviAII CATG 3 cut(s) 229, 457, 685
DdeI CTNAG 1 cut(s) 212
DpnI GATC 2 cut(s) 8, 920
DpnII GATC 2 cut(s) 6, 918
DraIII CACNNNGTG 3 cut(s) 208, 664, 892
EaeI YGGCCR 1 cut(s) 385
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
Eco130I CCWWGG 2 cut(s) 270, 498
Eco88I CYCGRG 1 cut(s) 149
EcoRII CCWGG 2 cut(s) 204, 393
EcoT14I CCWWGG 2 cut(s) 270, 498
ErhI CCWWGG 2 cut(s) 270, 498
Esp3I CGTCTC 1 cut(s) 677
FaeI CATG 3 cut(s) 232, 460, 688
FaiI YATR 9 cut(s) 230, 266, 458, 686, 752, 861, 914, 950, 1043
FatI CATG 3 cut(s) 228, 456, 684
FblI GTMKAC 1 cut(s) 445
Fnu4HI GCNGC 5 cut(s) 146, 374, 388, 830, 1058
FokI GGATG 6 cut(s) 167, 395, 623, 851, 1004, 1079
Fsp4HI GCNGC 5 cut(s) 146, 374, 388, 830, 1058
FspBI CTAG 1 cut(s) 605
GlaI GCGC 1 cut(s) 905
GluI GCNGC 5 cut(s) 146, 374, 388, 830, 1058
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 2 cut(s) 552, 780
HaeIII GGCC 1 cut(s) 387
HapII CCGG 2 cut(s) 483, 711
HgaI GACGC 2 cut(s) 168, 659
HhaI GCGC 1 cut(s) 906
Hin1II CATG 3 cut(s) 232, 460, 688
Hin6I GCGC 1 cut(s) 904
HinP1I GCGC 1 cut(s) 904
HpaII CCGG 2 cut(s) 483, 711
HphI GGTGA 4 cut(s) 31, 472, 487, 1106
Hpy166II GTNNAC 4 cut(s) 423, 446, 879, 1107
Hpy188III TCNNGA 8 cut(s) 320, 472, 548, 569, 700, 776, 797, 1004
Hpy8I GTNNAC 4 cut(s) 423, 446, 879, 1107
HpyAV CCTTC 8 cut(s) 95, 301, 437, 637, 837, 1093, 1121, 1133
HpyCH4IV ACGT 2 cut(s) 303, 448
HpyF10VI GCNNNNNNNGC 2 cut(s) 892, 901
HpyF3I CTNAG 1 cut(s) 212
HpySE526I ACGT 2 cut(s) 303, 448
Hsp92II CATG 3 cut(s) 232, 460, 688
HspAI GCGC 1 cut(s) 904
KspI CCGCGG 1 cut(s) 223
Kzo9I GATC 2 cut(s) 6, 918
Lsp1109I GCAGC 4 cut(s) 157, 385, 841, 1069
LweI GCATC 1 cut(s) 1026
MabI ACCWGGT 1 cut(s) 204
MaeI CTAG 1 cut(s) 605
MaeII ACGT 2 cut(s) 303, 448
MalI GATC 2 cut(s) 8, 920
MboI GATC 2 cut(s) 6, 918
MboII GAAGA 3 cut(s) 256, 647, 955
MfeI CAATTG 1 cut(s) 522
MflI RGATCY 2 cut(s) 6, 918
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 2 cut(s) 522, 771
MlyI GAGTC 9 cut(s) 13, 190, 210, 241, 348, 428, 884, 1069, 1112
MroXI GAANNNNTTC 1 cut(s) 105
MseI TTAA 1 cut(s) 15
MspA1I CMGCKG 1 cut(s) 222
MspI CCGG 2 cut(s) 483, 711
MspR9I CCNGG 2 cut(s) 206, 395
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 2 cut(s) 105, 561
MvaI CCWGG 2 cut(s) 206, 395
MvnI CGCG 2 cut(s) 222, 906
MwoI GCNNNNNNNGC 2 cut(s) 892, 901
NdeII GATC 2 cut(s) 6, 918
NlaIII CATG 3 cut(s) 232, 460, 688
NspI RCATGY 3 cut(s) 232, 460, 688
PaeI GCATGC 3 cut(s) 232, 460, 688
PaeR7I CTCGAG 1 cut(s) 149
PctI GAATGC 2 cut(s) 105, 561
PdmI GAANNNNTTC 1 cut(s) 105
PfeI GAWTC 5 cut(s) 191, 316, 544, 647, 1000
PflFI GACNNNGTC 1 cut(s) 74
PkrI GCNGC 5 cut(s) 147, 375, 389, 831, 1059
PleI GAGTC 9 cut(s) 13, 190, 210, 241, 348, 427, 883, 1069, 1111
PpsI GAGTC 9 cut(s) 13, 190, 210, 241, 348, 427, 883, 1069, 1111
Ppu21I YACGTR 1 cut(s) 449
Psp6I CCWGG 2 cut(s) 204, 393
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 2 cut(s) 204, 393
PspXI VCTCGAGB 1 cut(s) 149
PsuI RGATCY 2 cut(s) 6, 918
PsyI GACNNNGTC 1 cut(s) 74
SacII CCGCGG 1 cut(s) 223
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 5 cut(s) 146, 374, 388, 830, 1058
Sau3AI GATC 2 cut(s) 6, 918
SchI GAGTC 9 cut(s) 13, 190, 210, 241, 348, 428, 884, 1069, 1112
ScrFI CCNGG 2 cut(s) 206, 395
SduI GDGCHC 1 cut(s) 213
SexAI ACCWGGT 1 cut(s) 204
SfaNI GCATC 1 cut(s) 1026
Sfr274I CTCGAG 1 cut(s) 149
Sfr303I CCGCGG 1 cut(s) 223
SgrBI CCGCGG 1 cut(s) 223
SlaI CTCGAG 1 cut(s) 149
SmlI CTYRAG 5 cut(s) 44, 149, 377, 833, 1061
SmoI CTYRAG 5 cut(s) 44, 149, 377, 833, 1061
SphI GCATGC 3 cut(s) 232, 460, 688
Sse9I AATT 2 cut(s) 522, 771
SsiI CCGC 5 cut(s) 220, 222, 388, 626, 906
SspI AATATT 3 cut(s) 181, 637, 865
SspMI CTAG 1 cut(s) 605
StyD4I CCNGG 2 cut(s) 204, 393
StyI CCWWGG 2 cut(s) 270, 498
TaiI ACGT 2 cut(s) 306, 451
TaqI TCGA 3 cut(s) 69, 150, 194
TasI AATT 2 cut(s) 522, 771
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 5 cut(s) 191, 316, 544, 647, 1000
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 2 cut(s) 31, 259
TseI GCWGC 4 cut(s) 145, 373, 829, 1057
TspDTI ATGAA 1 cut(s) 419
TspGWI ACGGA 2 cut(s) 665, 1121
TspRI CASTG 2 cut(s) 31, 259
Tth111I GACNNNGTC 1 cut(s) 74
XapI RAATTY 1 cut(s) 771
XceI RCATGY 3 cut(s) 232, 460, 688
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XhoI CTCGAG 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 445
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.