FvH4_2g20281

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011635
Physical Location & Seq
Forward (+)
17052853 .. 17055666
2814 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20281.t1

Sequence Viewer

Length: 1374 bp
ATGCAGATCTTTGTGAAAACCCTCACAGGCAAGACCATCACCCTTGAGGTTGAAAGCTCTGACACTATTGACAATGTCAAAGCAAAGATCCAAGACAAGGAGGGAATTCCCCCGGACCAACAAAGGCTGATCTTTGCTGGCAAGCAGCTTGAGGACGGGCGGACACTCGCTGACTACAACATCCAAAAAGAGTCCACTCTCCACTTGGTTCTCAGACTCCGTGGTGGCATGCAGATCTTTGTTAAGACTCTCACAGGGAAGACCATCACATTGGAGGTTGAGAGTTCTGATACAATCGATAATGTCAAGGCAAAGATCCAGGACAAGGAGGGCATACCCCCAGATCAGCAGAGGCTCATCTTTGCCGGCAAACAGCTAGAGGATGGCCGCACCCTTGCCGATTACAATATCCAAAAGGAGTCTACTCTTCACCTGGTGCTCCGTCTGCGTGGTGGAATGCAGATCTTTGTAAAGACGCTTACTGGGAAGACAATCACCCTGGAGGTGGAAAGCTCCGATACAATTGACAATGTCAAGGCGAAGATCCAGGACAAGGAGGGTATTCCCCCAGATCAGCAGAGGCTCATCTTTGCAGGTAAACAGTTGGAGGACGGAAGAACTCTTGCAGACTACAACATTCAAAAGGAATCAACCTTGCACTTGGTTCTCCGACTGCGTGGTGGAATGCAGATCTTTGTAAAGACGCTTACTGGGAAGACAATCACCTTGGAGGTGGAAAGCTCCGATACAATTGACAATGTCAAGGCAAAAATCCAGGACAAGGAGGGTATCCCCCCAGATCAGCAGAGGCTCATCTTTGCAGGTAAACAGTTGGAGGACGGAAGAACTCTTGCAGACTACAACATTCAAAAGGAATCTACCTTGCACTTGGTTCTCCGTCTGCGTGGTGGAATGCAAATTTTTGTGAAGACTTTGACCGGGAAAACCATTACTTTGGAAGTGGAAAGCTCAGACACAATTGATAACGTCAAGGCAAAGATTCAGGACAAAGAAGGCATTCCCCCAGACCAGCAGAGGCTTATCTTTGCAGGAAAGCAGCTTGAGGACGGCAGGACACTGGCAGATTACAACATTCAGAAAGAGTCGACTCTGCATTTGGTGCTCCGTCTACGTGGTGGGATGCAGATCTTTGTGAAGACACTTACTGGAAAGACCATAACCTTGGAGGTAGAGAGTTCTGATACAATTGATAATGTGAAAGCAAAGATTCAGGACAAGGAAGGCATTCCTCCAGACCAGCAGAGGCTCATATTTGCTGGTAAGCAGCTTGAAGATGGGAGGACTCTGGCGGATTACAACATTCAAAAGGAGTCGACCCTTCACCTTGTCCTTCGCCTCCGTGGTGGTTTCTGA

Protein Analysis

458

Amino Acids

51.21

Weight (kDa)

7.05

Isoelectric Point (pI)

29.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 1.1e-16 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 74 1.4e-31 Ubiquitin family
Rad60-SLD PF11976 77 - 147 1.1e-16 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 79 - 150 1.4e-31 Ubiquitin family
Rad60-SLD PF11976 153 - 223 1.1e-16 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 155 - 226 1.4e-31 Ubiquitin family
Rad60-SLD PF11976 229 - 299 1.1e-16 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 231 - 302 1.4e-31 Ubiquitin family
Rad60-SLD PF11976 305 - 375 1.1e-16 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 307 - 378 1.4e-31 Ubiquitin family
Rad60-SLD PF11976 381 - 451 1.1e-16 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 383 - 454 1.4e-31 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 584, 812
AccI GTMKAC 4 cut(s) 422, 1106, 1129, 1334
AciI CCGC 3 cut(s) 160, 388, 1310
AclWI GGATC 3 cut(s) 82, 310, 538
AcoI YGGCCR 1 cut(s) 385
AcsI RAATTY 2 cut(s) 105, 918
AdeI CACNNNGTG 1 cut(s) 436
AfiI CCNNNNNNNGG 5 cut(s) 97, 325, 505, 553, 781
AgsI TTSAA 5 cut(s) 53, 641, 869, 1292, 1325
AjnI CCWGG 5 cut(s) 318, 432, 498, 546, 774
AluBI AGCT 8 cut(s) 57, 148, 376, 513, 741, 969, 1060, 1288
AluI AGCT 8 cut(s) 57, 148, 376, 513, 741, 969, 1060, 1288
Alw21I GWGCWC 2 cut(s) 441, 1125
AlwI GGATC 3 cut(s) 82, 310, 538
AoxI GGCC 1 cut(s) 385
ApeKI GCWGC 3 cut(s) 145, 1057, 1285
ApoI RAATTY 2 cut(s) 105, 918
ArsI GACNNNNNNTTYG 2 cut(s) 1099, 1131
Asp700I GAANNNNTTC 2 cut(s) 1017, 1245
AspS9I GGNCC 1 cut(s) 115
AsuC2I CCSGG 2 cut(s) 113, 940
AsuHPI GGTGA 5 cut(s) 31, 422, 487, 715, 1334
AvaII GGWCC 1 cut(s) 115
BbsI GAAGAC 5 cut(s) 266, 494, 722, 935, 1163
Bbv12I GWGCWC 2 cut(s) 441, 1125
BbvI GCAGC 3 cut(s) 157, 1069, 1297
BccI CCATC 4 cut(s) 44, 272, 377, 1289
BceAI ACGGC 1 cut(s) 1084
BciT130I CCWGG 5 cut(s) 320, 434, 500, 548, 776
BciVI GTATCC 1 cut(s) 800
BcnI CCSGG 2 cut(s) 113, 940
BfaI CTAG 1 cut(s) 377
BfuAI ACCTGC 2 cut(s) 584, 812
BfuI GTATCC 1 cut(s) 800
BglII AGATCT 5 cut(s) 6, 234, 462, 690, 1146
BisI GCNGC 4 cut(s) 146, 388, 1058, 1286
BlsI GCNGC 4 cut(s) 147, 389, 1059, 1287
Bme1390I CCNGG 7 cut(s) 113, 320, 434, 500, 548, 776, 940
Bme18I GGWCC 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 115
BmrFI CCNGG 7 cut(s) 113, 320, 434, 500, 548, 776, 940
BmrI ACTGGG 2 cut(s) 492, 720
BmsI GCATC 1 cut(s) 1131
BmuI ACTGGG 2 cut(s) 492, 720
BpiI GAAGAC 5 cut(s) 266, 494, 722, 935, 1163
BpmI CTGGAG 2 cut(s) 521, 1236
BpuEI CTTGAG 3 cut(s) 65, 170, 1082
BpuMI CCSGG 2 cut(s) 113, 940
Bsa29I ATCGAT 1 cut(s) 297
BsaAI YACGTR 1 cut(s) 1133
BsaBI GATNNNNATC 1 cut(s) 1145
BsaJI CCNNGG 6 cut(s) 111, 220, 498, 726, 1182, 1360
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 325, 505, 553, 781
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 4 cut(s) 487, 715, 1083, 1171
Bse8I GATNNNNATC 1 cut(s) 1145
BseBI CCWGG 5 cut(s) 320, 434, 500, 548, 776
BseCI ATCGAT 1 cut(s) 297
BseDI CCNNGG 6 cut(s) 111, 220, 498, 726, 1182, 1360
BseGI GGATG 3 cut(s) 180, 388, 1146
BseJI GATNNNNATC 1 cut(s) 1145
BseLI CCNNNNNNNGG 5 cut(s) 97, 325, 505, 553, 781
BseMII CTCAG 2 cut(s) 226, 984
BseNI ACTGG 4 cut(s) 487, 715, 1083, 1171
BseXI GCAGC 3 cut(s) 157, 1069, 1297
BshFI GGCC 1 cut(s) 387
BshVI ATCGAT 1 cut(s) 297
BsiHKAI GWGCWC 2 cut(s) 441, 1125
BsiSI CCGG 3 cut(s) 113, 366, 939
BslI CCNNNNNNNGG 5 cut(s) 97, 325, 505, 553, 781
BsmI GAATGC 5 cut(s) 462, 690, 918, 1017, 1245
BsnI GGCC 1 cut(s) 387
Bsp1286I GDGCHC 2 cut(s) 441, 1125
BspACI CCGC 3 cut(s) 160, 388, 1310
BspANI GGCC 1 cut(s) 387
BspCNI CTCAG 2 cut(s) 225, 983
BspDI ATCGAT 1 cut(s) 297
BspMI ACCTGC 2 cut(s) 584, 812
BspPI GGATC 3 cut(s) 82, 310, 538
BsrFI RCCGGY 1 cut(s) 365
BsrI ACTGG 4 cut(s) 487, 715, 1083, 1171
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 6 cut(s) 111, 220, 498, 726, 1182, 1360
BssT1I CCWWGG 2 cut(s) 726, 1182
Bst2UI CCWGG 5 cut(s) 320, 434, 500, 548, 776
Bst4CI ACNGT 2 cut(s) 603, 831
Bst6I CTCTTC 1 cut(s) 432
BstAPI GCANNNNNTGC 1 cut(s) 1120
BstBAI YACGTR 1 cut(s) 1133
BstC8I GCNNGC 4 cut(s) 139, 143, 230, 367
BstDEI CTNAG 2 cut(s) 212, 970
BstDSI CCRYGG 2 cut(s) 220, 1360
BstF5I GGATG 3 cut(s) 180, 388, 1146
BstMWI GCNNNNNNNGC 2 cut(s) 445, 1120
BstNI CCWGG 5 cut(s) 320, 434, 500, 548, 776
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 7 cut(s) 111, 318, 432, 498, 546, 774, 938
BstV1I GCAGC 3 cut(s) 157, 1069, 1297
BstV2I GAAGAC 5 cut(s) 266, 494, 722, 935, 1163
BstX2I RGATCY 8 cut(s) 6, 87, 234, 315, 462, 543, 690, 1146
BstXI CCANNNNNNTGG 3 cut(s) 271, 955, 1183
BstYI RGATCY 8 cut(s) 6, 87, 234, 315, 462, 543, 690, 1146
Bsu15I ATCGAT 1 cut(s) 297
BsuI GTATCC 1 cut(s) 800
BsuRI GGCC 1 cut(s) 387
BsuTUI ATCGAT 1 cut(s) 297
BtgI CCRYGG 2 cut(s) 220, 1360
BtsCI GGATG 3 cut(s) 180, 388, 1146
BtsIMutI CAGTG 1 cut(s) 1076
BveI ACCTGC 2 cut(s) 584, 812
Cac8I GCNNGC 4 cut(s) 139, 143, 230, 367
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 1 cut(s) 115
ClaI ATCGAT 1 cut(s) 297
CseI GACGC 2 cut(s) 484, 712
CsiI ACCWGGT 1 cut(s) 432
CviAII CATG 1 cut(s) 229
DdeI CTNAG 2 cut(s) 212, 970
DraIII CACNNNGTG 1 cut(s) 436
EaeI YGGCCR 1 cut(s) 385
Eam1104I CTCTTC 1 cut(s) 432
EarI CTCTTC 1 cut(s) 432
EciI GGCGGA 2 cut(s) 175, 1325
Eco130I CCWWGG 2 cut(s) 726, 1182
Eco47I GGWCC 1 cut(s) 115
EcoRI GAATTC 1 cut(s) 105
EcoRII CCWGG 5 cut(s) 318, 432, 498, 546, 774
EcoT14I CCWWGG 2 cut(s) 726, 1182
ErhI CCWWGG 2 cut(s) 726, 1182
FaeI CATG 1 cut(s) 232
FaiI YATR 4 cut(s) 230, 335, 1178, 1271
FatI CATG 1 cut(s) 228
FblI GTMKAC 4 cut(s) 422, 1106, 1129, 1334
Fnu4HI GCNGC 4 cut(s) 146, 388, 1058, 1286
FokI GGATG 3 cut(s) 167, 395, 1153
Fsp4HI GCNGC 4 cut(s) 146, 388, 1058, 1286
FspBI CTAG 1 cut(s) 377
GluI GCNGC 4 cut(s) 146, 388, 1058, 1286
GsuI CTGGAG 2 cut(s) 521, 1236
HaeIII GGCC 1 cut(s) 387
HapII CCGG 3 cut(s) 113, 366, 939
HgaI GACGC 2 cut(s) 484, 712
Hin1II CATG 1 cut(s) 232
HincII GTYRAC 2 cut(s) 1107, 1335
HindII GTYRAC 2 cut(s) 1107, 1335
HpaII CCGG 3 cut(s) 113, 366, 939
HphI GGTGA 5 cut(s) 31, 422, 487, 715, 1334
Hpy166II GTNNAC 7 cut(s) 195, 423, 599, 827, 1107, 1130, 1335
Hpy188III TCNNGA 3 cut(s) 1004, 1232, 1253
Hpy8I GTNNAC 7 cut(s) 195, 423, 599, 827, 1107, 1130, 1335
HpyAV CCTTC 4 cut(s) 1007, 1235, 1349, 1361
HpyCH4III ACNGT 2 cut(s) 603, 831
HpyCH4IV ACGT 2 cut(s) 987, 1132
HpyF10VI GCNNNNNNNGC 2 cut(s) 445, 1120
HpyF3I CTNAG 2 cut(s) 212, 970
HpySE526I ACGT 2 cut(s) 987, 1132
Hsp92II CATG 1 cut(s) 232
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
LmnI GCTCC 4 cut(s) 444, 518, 746, 1128
Lsp1109I GCAGC 3 cut(s) 157, 1069, 1297
LweI GCATC 1 cut(s) 1131
MabI ACCWGGT 1 cut(s) 432
MaeI CTAG 1 cut(s) 377
MaeII ACGT 2 cut(s) 987, 1132
MfeI CAATTG 4 cut(s) 522, 750, 978, 1206
MflI RGATCY 8 cut(s) 6, 87, 234, 315, 462, 543, 690, 1146
MhlI GDGCHC 2 cut(s) 441, 1125
MluCI AATT 6 cut(s) 105, 522, 750, 918, 978, 1206
MlyI GAGTC 8 cut(s) 200, 210, 241, 428, 1102, 1112, 1297, 1340
MmeI TCCRAC 3 cut(s) 585, 694, 813
MroNI GCCGGC 1 cut(s) 365
MroXI GAANNNNTTC 2 cut(s) 1017, 1245
MseI TTAA 1 cut(s) 243
MspI CCGG 3 cut(s) 113, 366, 939
MspR9I CCNGG 7 cut(s) 113, 320, 434, 500, 548, 776, 940
MunI CAATTG 4 cut(s) 522, 750, 978, 1206
Mva1269I GAATGC 5 cut(s) 462, 690, 918, 1017, 1245
MvaI CCWGG 5 cut(s) 320, 434, 500, 548, 776
MwoI GCNNNNNNNGC 2 cut(s) 445, 1120
NaeI GCCGGC 1 cut(s) 367
NciI CCSGG 2 cut(s) 113, 940
NgoMIV GCCGGC 1 cut(s) 365
NlaIII CATG 1 cut(s) 232
NspI RCATGY 1 cut(s) 232
PaeI GCATGC 1 cut(s) 232
PctI GAATGC 5 cut(s) 462, 690, 918, 1017, 1245
PdiI GCCGGC 1 cut(s) 367
PdmI GAANNNNTTC 2 cut(s) 1017, 1245
PfeI GAWTC 4 cut(s) 647, 875, 1000, 1228
PflFI GACNNNGTC 3 cut(s) 74, 530, 758
PfoI TCCNGGA 3 cut(s) 318, 546, 774
PkrI GCNGC 4 cut(s) 147, 389, 1059, 1287
PleI GAGTC 8 cut(s) 199, 210, 241, 427, 1102, 1111, 1297, 1339
PpsI GAGTC 8 cut(s) 199, 210, 241, 427, 1102, 1111, 1297, 1339
Ppu21I YACGTR 1 cut(s) 1133
Psp6I CCWGG 5 cut(s) 318, 432, 498, 546, 774
PspGI CCWGG 5 cut(s) 318, 432, 498, 546, 774
PspPI GGNCC 1 cut(s) 115
PsuI RGATCY 8 cut(s) 6, 87, 234, 315, 462, 543, 690, 1146
PsyI GACNNNGTC 3 cut(s) 74, 530, 758
SalI GTCGAC 2 cut(s) 1105, 1333
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 4 cut(s) 146, 388, 1058, 1286
Sau96I GGNCC 1 cut(s) 115
SchI GAGTC 8 cut(s) 200, 210, 241, 428, 1102, 1112, 1297, 1340
ScrFI CCNGG 7 cut(s) 113, 320, 434, 500, 548, 776, 940
SduI GDGCHC 2 cut(s) 441, 1125
SexAI ACCWGGT 1 cut(s) 432
SfaNI GCATC 1 cut(s) 1131
SinI GGWCC 1 cut(s) 115
SmlI CTYRAG 3 cut(s) 44, 149, 1061
SmoI CTYRAG 3 cut(s) 44, 149, 1061
SphI GCATGC 1 cut(s) 232
Sse9I AATT 6 cut(s) 105, 522, 750, 918, 978, 1206
SsiI CCGC 3 cut(s) 160, 388, 1310
SspMI CTAG 1 cut(s) 377
StyD4I CCNGG 7 cut(s) 111, 318, 432, 498, 546, 774, 938
StyI CCWWGG 2 cut(s) 726, 1182
TaaI ACNGT 2 cut(s) 603, 831
TaiI ACGT 2 cut(s) 990, 1135
TaqI TCGA 3 cut(s) 297, 1106, 1334
TasI AATT 6 cut(s) 105, 522, 750, 918, 978, 1206
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 4 cut(s) 647, 875, 1000, 1228
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 1 cut(s) 1083
TseI GCWGC 3 cut(s) 145, 1057, 1285
TspGWI ACGGA 7 cut(s) 209, 431, 627, 855, 887, 1115, 1349
TspRI CASTG 1 cut(s) 1083
Tth111I GACNNNGTC 3 cut(s) 74, 530, 758
VpaK11BI GGWCC 1 cut(s) 115
XapI RAATTY 2 cut(s) 105, 918
XceI RCATGY 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XmiI GTMKAC 4 cut(s) 422, 1106, 1129, 1334
XmnI GAANNNNTTC 2 cut(s) 1017, 1245
XspI CTAG 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.