Rw5G023750

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011635
Physical Location & Seq
Reverse (-)
33611332 .. 33613405
2074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023750.1

Sequence Viewer

Length: 1146 bp
ATGCAGATCTTCGTCAAAACTCTCACTGGCAAGACCATAACCCTTGAGGTTGAAAGCTCTGACACCATAGATAATGTCAAAGCCAAAATCCAAGACAAGGAAGGCATCCCCCCGGACCAGCAAAGGCTGATTTTTGCTGGCAAGCAGCTCGAGGATGGACGCACCCTCGCTGACTACAATATTCAAAAGGAATCCACTCTTCACCTGGTGCTCAGACTCCGCGGTGGCATGCAAATCTTTGTTAAGACTCTCACTGGGAAAACCATAACCTTGGAGGTTGAGAGTTCCGACACCATTGACAATGTGAAGGCAAAGATCCAAGACAAAGAGGGTATCCCACCAGACCAGCAGAGACTTATCTTTGCTGGAAAGCAACTTGAGGATGGCCGCACCCTGGCTGATTACAATATTCAAAAGGAGTCGACCCTTCATTTAGTGCTCCGTCTTCGTGGTGGCATGCAAATCTTTGTCAAAACCCTCACCGGAAAGACCATCACCTTGGAGGTTGAAAGTTCAGATACAATAGATAATGTCAAGGCAAAGATTCAGGATAAGGAGGGCATTCCTCCAGACCAGCAGAGGCTCATCTTTGCAGGGAAACAGTTAGAGGATGGTCGCACTCTCGCGGATTACAACATCCAGAAAGAATCAACTCTTCACTTGGTGTTGCGTCTTCGTGGTGGCATGCAAATCTTTGTCAAGACCCTCACCGGCAAAACCATCACTCTGGAAGTGGAAAGCTCTGACACGATTGATAATGTGAAAGCTAAAATTCAGGACAAAGAGGGTATTCCCCCAGACCAGCAGAGATTGATTTTTGCAGGTAAGCAGCTTGAGGATGGAAGGACACTTGCCGACTATAATATTCAGAAGGAGTCCACTCTGCACTTGGTGCTTCGTCTGCGTGGTGGTATGCAGATCTTTGTTAAAACTCTGACTGGGAAGACCATTACTTTGGAGGTAGAGAGTTCAGATACGATTGACAATGTAAAAACCAAGATTCAGGACAAGGAGGGCATTCCTCCGGACCAGCAGAGGCTCATATTTGCTGGAAAGCAGCTTGAGGATGGGAGGACCCTTGCTGACTATAACATCCAGAAGGAGTCCACCCTTCACCTTGTCCTCCGCCTCCGCGGTGGTTTCTGA

Protein Analysis

381

Amino Acids

42.73

Weight (kDa)

7.0

Isoelectric Point (pI)

29.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 74 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 77 - 147 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 79 - 150 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 153 - 223 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 155 - 226 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 229 - 299 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 231 - 302 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 305 - 375 8.6e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 307 - 378 2.7e-31 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 812
AccI GTMKAC 1 cut(s) 422
AccII CGCG 3 cut(s) 222, 626, 1134
AccIII TCCGGA 1 cut(s) 1024
AciI CCGC 7 cut(s) 220, 222, 388, 626, 1126, 1132, 1134
AclWI GGATC 1 cut(s) 310
AcoI YGGCCR 1 cut(s) 385
AcsI RAATTY 1 cut(s) 771
AdeI CACNNNGTG 3 cut(s) 208, 664, 892
AfiI CCNNNNNNNGG 2 cut(s) 97, 394
AgsI TTSAA 4 cut(s) 53, 185, 413, 509
AjnI CCWGG 2 cut(s) 204, 393
AluBI AGCT 6 cut(s) 57, 148, 741, 767, 832, 1060
AluI AGCT 6 cut(s) 57, 148, 741, 767, 832, 1060
Alw21I GWGCWC 2 cut(s) 213, 441
Alw26I GTCTC 1 cut(s) 346
AlwI GGATC 1 cut(s) 310
Ama87I CYCGRG 1 cut(s) 149
Aor13HI TCCGGA 1 cut(s) 1024
AoxI GGCC 1 cut(s) 385
ApeKI GCWGC 3 cut(s) 145, 829, 1057
ApoI RAATTY 1 cut(s) 771
ArsI GACNNNNNNTTYG 2 cut(s) 937, 969
AspS9I GGNCC 3 cut(s) 115, 1027, 1074
AsuC2I CCSGG 1 cut(s) 113
AsuHPI GGTGA 5 cut(s) 194, 472, 487, 700, 1106
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 3 cut(s) 115, 1027, 1074
BbsI GAAGAC 3 cut(s) 437, 665, 950
Bbv12I GWGCWC 2 cut(s) 213, 441
BbvI GCAGC 3 cut(s) 157, 841, 1069
BccI CCATC 7 cut(s) 149, 377, 500, 605, 728, 833, 1061
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 2 cut(s) 206, 395
BciVI GTATCC 1 cut(s) 344
BcnI CCSGG 1 cut(s) 113
BcoDI GTCTC 1 cut(s) 346
BfuAI ACCTGC 1 cut(s) 812
BfuI GTATCC 1 cut(s) 344
BglII AGATCT 2 cut(s) 6, 918
BisI GCNGC 4 cut(s) 146, 388, 830, 1058
BlsI GCNGC 4 cut(s) 147, 389, 831, 1059
Bme1390I CCNGG 3 cut(s) 113, 206, 395
Bme18I GGWCC 3 cut(s) 115, 1027, 1074
BmeT110I CYCGRG 1 cut(s) 149
BmgT120I GGNCC 3 cut(s) 115, 1027, 1074
BmiI GGNNCC 1 cut(s) 1076
BmrFI CCNGG 3 cut(s) 113, 206, 395
BmrI ACTGGG 2 cut(s) 264, 948
BmsI GCATC 1 cut(s) 114
BmuI ACTGGG 2 cut(s) 264, 948
BpiI GAAGAC 3 cut(s) 437, 665, 950
BpmI CTGGAG 1 cut(s) 552
BpuEI CTTGAG 4 cut(s) 65, 398, 854, 1082
BpuMI CCSGG 1 cut(s) 113
BsaJI CCNNGG 6 cut(s) 111, 220, 270, 393, 498, 1132
BsaWI WCCGGW 2 cut(s) 482, 1024
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 394
Bse118I RCCGGY 1 cut(s) 710
Bse1I ACTGG 3 cut(s) 31, 259, 943
BseAI TCCGGA 1 cut(s) 1024
BseBI CCWGG 2 cut(s) 206, 395
BseDI CCNNGG 6 cut(s) 111, 220, 270, 393, 498, 1132
BseGI GGATG 8 cut(s) 105, 160, 388, 616, 636, 844, 1072, 1092
BseLI CCNNNNNNNGG 2 cut(s) 97, 394
BseMII CTCAG 1 cut(s) 226
BseNI ACTGG 3 cut(s) 31, 259, 943
BseXI GCAGC 3 cut(s) 157, 841, 1069
BsgI GTGCAG 1 cut(s) 869
Bsh1236I CGCG 3 cut(s) 222, 626, 1134
BshFI GGCC 1 cut(s) 387
BsiHKAI GWGCWC 2 cut(s) 213, 441
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 4 cut(s) 113, 483, 711, 1025
BslI CCNNNNNNNGG 2 cut(s) 97, 394
BsmAI GTCTC 1 cut(s) 346
BsmI GAATGC 2 cut(s) 561, 1017
BsnI GGCC 1 cut(s) 387
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 2 cut(s) 213, 441
Bsp13I TCCGGA 1 cut(s) 1024
Bsp143I GATC 3 cut(s) 6, 315, 918
BspACI CCGC 7 cut(s) 220, 222, 388, 626, 1126, 1132, 1134
BspANI GGCC 1 cut(s) 387
BspCNI CTCAG 1 cut(s) 225
BspEI TCCGGA 1 cut(s) 1024
BspFNI CGCG 3 cut(s) 222, 626, 1134
BspLI GGNNCC 1 cut(s) 1076
BspMI ACCTGC 1 cut(s) 812
BspPI GGATC 1 cut(s) 310
BsrFI RCCGGY 1 cut(s) 710
BsrI ACTGG 3 cut(s) 31, 259, 943
BssAI RCCGGY 1 cut(s) 710
BssECI CCNNGG 6 cut(s) 111, 220, 270, 393, 498, 1132
BssMI GATC 3 cut(s) 6, 315, 918
BssT1I CCWWGG 2 cut(s) 270, 498
Bst2UI CCWGG 2 cut(s) 206, 395
Bst4CI ACNGT 1 cut(s) 603
Bst6I CTCTTC 2 cut(s) 204, 660
BstAPI GCANNNNNTGC 1 cut(s) 892
BstC8I GCNNGC 5 cut(s) 139, 143, 230, 458, 686
BstDEI CTNAG 1 cut(s) 212
BstDSI CCRYGG 2 cut(s) 220, 1132
BstF5I GGATG 8 cut(s) 105, 160, 388, 616, 636, 844, 1072, 1092
BstFNI CGCG 3 cut(s) 222, 626, 1134
BstKTI GATC 3 cut(s) 9, 318, 921
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 3 cut(s) 6, 315, 918
BstMWI GCNNNNNNNGC 2 cut(s) 892, 901
BstNI CCWGG 2 cut(s) 206, 395
BstNSI RCATGY 3 cut(s) 232, 460, 688
BstSCI CCNGG 3 cut(s) 111, 204, 393
BstUI CGCG 3 cut(s) 222, 626, 1134
BstV1I GCAGC 3 cut(s) 157, 841, 1069
BstV2I GAAGAC 3 cut(s) 437, 665, 950
BstX2I RGATCY 3 cut(s) 6, 315, 918
BstXI CCANNNNNNTGG 4 cut(s) 271, 499, 727, 955
BstYI RGATCY 3 cut(s) 6, 315, 918
BsuI GTATCC 1 cut(s) 344
BsuRI GGCC 1 cut(s) 387
BtgI CCRYGG 2 cut(s) 220, 1132
BtsCI GGATG 8 cut(s) 105, 160, 388, 616, 636, 844, 1072, 1092
BtsIMutI CAGTG 2 cut(s) 24, 252
BveI ACCTGC 1 cut(s) 812
Cac8I GCNNGC 5 cut(s) 139, 143, 230, 458, 686
Cfr10I RCCGGY 1 cut(s) 710
Cfr13I GGNCC 3 cut(s) 115, 1027, 1074
Cfr42I CCGCGG 2 cut(s) 223, 1135
CseI GACGC 2 cut(s) 168, 659
CsiI ACCWGGT 1 cut(s) 204
CviAII CATG 3 cut(s) 229, 457, 685
DdeI CTNAG 1 cut(s) 212
DpnI GATC 3 cut(s) 8, 317, 920
DpnII GATC 3 cut(s) 6, 315, 918
DraIII CACNNNGTG 3 cut(s) 208, 664, 892
EaeI YGGCCR 1 cut(s) 385
Eam1104I CTCTTC 2 cut(s) 204, 660
EarI CTCTTC 2 cut(s) 204, 660
EciI GGCGGA 1 cut(s) 1115
Eco130I CCWWGG 2 cut(s) 270, 498
Eco47I GGWCC 3 cut(s) 115, 1027, 1074
Eco88I CYCGRG 1 cut(s) 149
EcoO109I RGGNCCY 1 cut(s) 1074
EcoRII CCWGG 2 cut(s) 204, 393
EcoT14I CCWWGG 2 cut(s) 270, 498
ErhI CCWWGG 2 cut(s) 270, 498
FaeI CATG 3 cut(s) 232, 460, 688
FatI CATG 3 cut(s) 228, 456, 684
FblI GTMKAC 1 cut(s) 422
Fnu4HI GCNGC 4 cut(s) 146, 388, 830, 1058
FokI GGATG 8 cut(s) 92, 167, 395, 623, 623, 851, 1079, 1079
Fsp4HI GCNGC 4 cut(s) 146, 388, 830, 1058
GluI GCNGC 4 cut(s) 146, 388, 830, 1058
GsuI CTGGAG 1 cut(s) 552
HaeIII GGCC 1 cut(s) 387
HapII CCGG 4 cut(s) 113, 483, 711, 1025
HgaI GACGC 2 cut(s) 168, 659
Hin1II CATG 3 cut(s) 232, 460, 688
HincII GTYRAC 1 cut(s) 423
HindII GTYRAC 1 cut(s) 423
HinfI GANTC 9 cut(s) 191, 216, 247, 419, 544, 647, 875, 1000, 1103
HpaII CCGG 4 cut(s) 113, 483, 711, 1025
HphI GGTGA 5 cut(s) 194, 472, 487, 700, 1106
Hpy166II GTNNAC 3 cut(s) 423, 879, 1107
Hpy188I TCNGA 9 cut(s) 61, 215, 289, 517, 745, 870, 936, 973, 1145
Hpy188III TCNNGA 9 cut(s) 548, 569, 640, 700, 728, 776, 1004, 1025, 1096
Hpy8I GTNNAC 3 cut(s) 423, 879, 1107
HpyAV CCTTC 7 cut(s) 95, 301, 437, 837, 865, 1093, 1121
HpyCH4III ACNGT 1 cut(s) 603
HpyCH4V TGCA 8 cut(s) 4, 232, 460, 593, 688, 821, 886, 916
HpyF10VI GCNNNNNNNGC 2 cut(s) 892, 901
HpyF3I CTNAG 1 cut(s) 212
Hsp92II CATG 3 cut(s) 232, 460, 688
Kpn2I TCCGGA 1 cut(s) 1024
KspI CCGCGG 2 cut(s) 223, 1135
Kzo9I GATC 3 cut(s) 6, 315, 918
LmnI GCTCC 1 cut(s) 444
Lsp1109I GCAGC 3 cut(s) 157, 841, 1069
LweI GCATC 1 cut(s) 114
MabI ACCWGGT 1 cut(s) 204
MalI GATC 3 cut(s) 8, 317, 920
MboI GATC 3 cut(s) 6, 315, 918
MboII GAAGA 5 cut(s) 191, 437, 647, 665, 955
MflI RGATCY 3 cut(s) 6, 315, 918
MhlI GDGCHC 2 cut(s) 213, 441
MluCI AATT 1 cut(s) 771
MlyI GAGTC 5 cut(s) 210, 241, 428, 884, 1112
MmeI TCCRAC 1 cut(s) 312
MroI TCCGGA 1 cut(s) 1024
MseI TTAA 2 cut(s) 243, 927
MspA1I CMGCKG 2 cut(s) 222, 1134
MspI CCGG 4 cut(s) 113, 483, 711, 1025
MspR9I CCNGG 3 cut(s) 113, 206, 395
Mva1269I GAATGC 2 cut(s) 561, 1017
MvaI CCWGG 2 cut(s) 206, 395
MvnI CGCG 3 cut(s) 222, 626, 1134
MwoI GCNNNNNNNGC 2 cut(s) 892, 901
NciI CCSGG 1 cut(s) 113
NdeII GATC 3 cut(s) 6, 315, 918
NlaIII CATG 3 cut(s) 232, 460, 688
NlaIV GGNNCC 1 cut(s) 1076
NspI RCATGY 3 cut(s) 232, 460, 688
PaeI GCATGC 3 cut(s) 232, 460, 688
PaeR7I CTCGAG 1 cut(s) 149
PctI GAATGC 2 cut(s) 561, 1017
PfeI GAWTC 4 cut(s) 191, 544, 647, 1000
PkrI GCNGC 4 cut(s) 147, 389, 831, 1059
PleI GAGTC 5 cut(s) 210, 241, 427, 883, 1111
PpsI GAGTC 5 cut(s) 210, 241, 427, 883, 1111
PpuMI RGGWCCY 1 cut(s) 1074
Psp5II RGGWCCY 1 cut(s) 1074
Psp6I CCWGG 2 cut(s) 204, 393
PspGI CCWGG 2 cut(s) 204, 393
PspN4I GGNNCC 1 cut(s) 1076
PspPI GGNCC 3 cut(s) 115, 1027, 1074
PspPPI RGGWCCY 1 cut(s) 1074
PspXI VCTCGAGB 1 cut(s) 149
PsuI RGATCY 3 cut(s) 6, 315, 918
SacII CCGCGG 2 cut(s) 223, 1135
SalI GTCGAC 1 cut(s) 421
SaqAI TTAA 2 cut(s) 243, 927
SatI GCNGC 4 cut(s) 146, 388, 830, 1058
Sau3AI GATC 3 cut(s) 6, 315, 918
Sau96I GGNCC 3 cut(s) 115, 1027, 1074
SchI GAGTC 5 cut(s) 210, 241, 428, 884, 1112
ScrFI CCNGG 3 cut(s) 113, 206, 395
SduI GDGCHC 2 cut(s) 213, 441
SexAI ACCWGGT 1 cut(s) 204
SfaNI GCATC 1 cut(s) 114
Sfr274I CTCGAG 1 cut(s) 149
Sfr303I CCGCGG 2 cut(s) 223, 1135
SgrBI CCGCGG 2 cut(s) 223, 1135
SinI GGWCC 3 cut(s) 115, 1027, 1074
SlaI CTCGAG 1 cut(s) 149
SmlI CTYRAG 5 cut(s) 44, 149, 377, 833, 1061
SmoI CTYRAG 5 cut(s) 44, 149, 377, 833, 1061
SphI GCATGC 3 cut(s) 232, 460, 688
Sse9I AATT 1 cut(s) 771
SsiI CCGC 7 cut(s) 220, 222, 388, 626, 1126, 1132, 1134
SspI AATATT 3 cut(s) 181, 409, 865
StyD4I CCNGG 3 cut(s) 111, 204, 393
StyI CCWWGG 2 cut(s) 270, 498
TaaI ACNGT 1 cut(s) 603
TaqI TCGA 2 cut(s) 150, 422
TasI AATT 1 cut(s) 771
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 4 cut(s) 191, 544, 647, 1000
Tru1I TTAA 2 cut(s) 243, 927
Tru9I TTAA 2 cut(s) 243, 927
TscAI CASTG 2 cut(s) 31, 259
TseI GCWGC 3 cut(s) 145, 829, 1057
TspDTI ATGAA 1 cut(s) 419
TspGWI ACGGA 1 cut(s) 431
TspRI CASTG 2 cut(s) 31, 259
VpaK11BI GGWCC 3 cut(s) 115, 1027, 1074
XapI RAATTY 1 cut(s) 771
XceI RCATGY 3 cut(s) 232, 460, 688
XcmI CCANNNNNNNNNTGG 2 cut(s) 202, 886
XhoI CTCGAG 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.