AT4G09630

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005063
Physical Location & Seq
Forward (+)
6083585 .. 6088134
4550 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G09630.2

Sequence Viewer

Length: 2136 bp
ATGGCTCAGTATAGACAATCAGGAACAGAGAGGTTTTTGGGAGGTCGAAGCGGTTACAATCCTTATGTCAACGGTGGAGCTTCGTCAGATCACGTAGCGATCGGGATCCGTAACGGCGGTGGTAGTAATTTCGGAGTTGTAACTCAGCACGGGAAAGCTAGTCGATGGCGGCGATCTACTCGATTAGATAGGAATCGTCGTTGTGGTGTTGGCTCTTTGGTGTTTGTTCTTTGTGTTGTGCTCGTTGTTTGTGTCTCAGCTTACTATTATTTCTCTGGTTATACTAATTTCGGCAAAGATGATAAAGGAATTGATACAGCTGAAGGTGATTTTCTTACGAATGTGTCAAGAACTGATCCTTTGAAAATACTTAAGTTTGGTCATGGTTCAGTGTTACATGGAAGAGATTCGAGAGATTGGGATAAGGATGATAGAAGAAGGGATGAAGATTATAATGAGGATGATGTAGAGGATAAATCTGTTGTTGAGGAGAGGTCAGTGTCTGAAGTGAAAAAGGATGTATCGCTTCGGAATCCTTTGAAGGGTTCGGATTGGAAGGGGGTTGGTTTTTACAATGAAGCTGGACGTGATGAGCTGAAGAAGTATGAAGTGGAGTATCAGGCTTCTCTTGTAAAAGGTGGTCAATCGTTGAAAGAGAATGATGGGCATCATCAGCCATTTGATACAGAATCTAATGAAGATGATTCGATTGATTCTCATGATACTCAAGGGGATGAATATGTTGACATGGGGCATGATGGGGATGAAAATGAAGAATCACACAAAGATAATCATAAGCACAATGAGGATGGTGCTGAAGAATCACACAAAGATAGTCATAGGCACAATGAGGATGGTGCTGAAGAATCTCACAAAGAGACTGCATCTGTGTTTCTTCATTCCACGACCAAGCATCAGAAAATTGAAAAGGTTCATGGAGCTACTTCAAAAAGGTCACGTGGGAAGTCTTCTCTTCTTAGTGGTAAGTCGGGGAAAACATCGCAAACTGATGCAAAAAGAAGAGCTCGAAGTCACAGATTTTCAGGCGTATCTTGTGAGATGAAGCTGTTGAATTCCAGTCAACAAATACAAGAGCCTTTGAAAACTCAAAATTTTGCTGCACCCTCCTTACAATACATACAGATGGAGGATAAACCTGATGGAGAAGAACAGTGGGAGCCTAAATTTGCAGGTCACCAGAGTTTACAGGAACGAGAAGACTCTTTCTTGGTTCAAGAGCAGAAAATCCATTGTGGCTTTGTCAAAGCTCCTGAAGGATTGCCAAGCACTGGATTTGACCTGACAGAAGATGATGCTAACTATATCAGTAAATGTCACATTGCTGTGATATCCTGTATCTTTGGAAACTCTGATCGCTTGAGGCATCCTGCTAATAAAATGGTAAGTAGTTTGTCAAGAAAAGATGTTTGCTTCGTTGTGTTTGTGGACGAGATTACCATGCAAACACTTTCTGCCGAAGGCCAAGTACCCGACGGAGCTGGATTCGTTGGTCTGTGGAAGTTGGTTGTGGTGAGGAATCTTCCTTACACAGATATGCGGAGGGTAGGGAAAATACCAAAACTGTTACCACACAGACTTTTCACTTCGGCAAGGTACTCAATCTGGTTGGACAGCAAGTTACGTCTCCAGTTGGACCCCCTAGTCATTTTGGAATACTTTCTGTGGCGTGAAGGGCACGAGTATGCTATCTCCAATCACTATGACCGCCATTGCCTATGGGAAGAAGTTGCACAAAACAAGAAGCTGAACAAGTACAATCACACTGTCATTGATCAGCAGTTTGAGTTTTACCAGTCTGACGGGCTGACGAGATTCAATGCTTCAGATCCCCACAAGCTTCTTCCCAGCAATGTTCCAGAAGGGTCTTTCATTGTACGGGAACATACCCCCATGTCAAACCTATTCTCCTGTCTTTGGTTCAACGAAGTTGAGCGCTTCACTCCCCGAGATCAGCTAAGCTTTGCATACACTTACCAGAAATTAACAAGGATGAATCCTGACACTCCATTTAATCTTCACATGTTCAAGGATTGCGAGAGGAGAAAAATCACCAAATTGTTTCGTCACAGATCAGAGGAGAAGCGAAACCTTATACAAGCAGCAACAACACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

711

Amino Acids

81.27

Weight (kDa)

6.37

Isoelectric Point (pI)

48.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 362 - 681 2.1e-143 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 453
AasI GACNNNNNNGTC 1 cut(s) 1661
Acc36I ACCTGC 1 cut(s) 1181
AccB7I CCANNNNNTGG 1 cut(s) 1289
AciI CCGC 5 cut(s) 51, 117, 169, 1558, 1726
AclWI GGATC 4 cut(s) 100, 113, 350, 1841
AcsI RAATTY 3 cut(s) 1072, 1111, 1184
AcuI CTGAAG 7 cut(s) 342, 525, 617, 837, 882, 1293, 1827
AcvI CACGTG 1 cut(s) 959
AfaI GTAC 4 cut(s) 1488, 1616, 1775, 1896
AfeI AGCGCT 1 cut(s) 1955
AfiI CCNNNNNNNGG 3 cut(s) 542, 1289, 1935
AflII CTTAAG 1 cut(s) 371
AflIII ACRYGT 1 cut(s) 2040
AjiI CACGTC 1 cut(s) 587
Alw21I GWGCWC 2 cut(s) 243, 1027
Alw26I GTCTC 3 cut(s) 259, 872, 1649
AlwI GGATC 4 cut(s) 100, 113, 350, 1841
AlwNI CAGNNNCTG 1 cut(s) 503
Ama87I CYCGRG 1 cut(s) 1965
Aor51HI AGCGCT 1 cut(s) 1955
AoxI GGCC 1 cut(s) 1480
ApeKI GCWGC 2 cut(s) 1118, 2120
ApoI RAATTY 3 cut(s) 1072, 1111, 1184
Asp700I GAANNNNTTC 3 cut(s) 406, 930, 1677
AspLEI GCGC 1 cut(s) 1956
AspS9I GGNCC 1 cut(s) 1654
AsuHPI GGTGA 4 cut(s) 338, 1187, 1543, 2062
AvaI CYCGRG 1 cut(s) 1965
AvaII GGWCC 1 cut(s) 1654
BaeGI GKGCMC 1 cut(s) 1698
BamHI GGATCC 1 cut(s) 105
BanII GRGCYC 1 cut(s) 1027
BarI GAAGNNNNNNTAC 2 cut(s) 1470, 1502
BauI CACGAG 1 cut(s) 1697
BbrPI CACGTG 1 cut(s) 959
BbsI GAAGAC 2 cut(s) 960, 1224
Bbv12I GWGCWC 2 cut(s) 243, 1027
BbvI GCAGC 2 cut(s) 1105, 2132
BccI CCATC 7 cut(s) 159, 656, 752, 803, 848, 1138, 1154
BceAI ACGGC 1 cut(s) 130
BclI TGATCA 1 cut(s) 1792
BcoDI GTCTC 3 cut(s) 259, 872, 1649
BfaI CTAG 2 cut(s) 159, 1661
BfoI RGCGCY 1 cut(s) 1957
BfrI CTTAAG 1 cut(s) 371
BfuAI ACCTGC 1 cut(s) 1181
BisI GCNGC 3 cut(s) 170, 1119, 2121
BlpI GCTNAGC 1 cut(s) 1976
BlsI GCNGC 3 cut(s) 171, 1120, 2122
Bme18I GGWCC 1 cut(s) 1654
BmeT110I CYCGRG 1 cut(s) 1965
BmgBI CACGTC 1 cut(s) 587
BmgT120I GGNCC 1 cut(s) 1654
BmiI GGNNCC 3 cut(s) 107, 1179, 1656
BmsI GCATC 6 cut(s) 676, 893, 922, 1000, 1303, 1393
BpiI GAAGAC 2 cut(s) 960, 1224
BpmI CTGGAG 1 cut(s) 1631
Bpu1102I GCTNAGC 1 cut(s) 1976
BpuEI CTTGAG 2 cut(s) 711, 1399
BsaAI YACGTR 2 cut(s) 94, 959
BsaBI GATNNNNATC 3 cut(s) 104, 192, 666
BsaXI ACNNNNNCTCC 2 cut(s) 1910, 1940
Bsc4I CCNNNNNNNGG 3 cut(s) 542, 1289, 1935
Bse1I ACTGG 4 cut(s) 1077, 1294, 1648, 1813
Bse3DI GCAATG 3 cut(s) 1338, 1729, 1876
Bse8I GATNNNNATC 3 cut(s) 104, 192, 666
BseJI GATNNNNATC 3 cut(s) 104, 192, 666
BseLI CCNNNNNNNGG 3 cut(s) 542, 1289, 1935
BseMI GCAATG 3 cut(s) 1338, 1729, 1876
BseMII CTCAG 3 cut(s) 20, 158, 270
BseNI ACTGG 4 cut(s) 1077, 1294, 1648, 1813
BseRI GAGGAG 3 cut(s) 503, 2074, 2111
BseSI GKGCMC 1 cut(s) 1698
BseXI GCAGC 2 cut(s) 1105, 2132
BseYI CCCAGC 1 cut(s) 1865
BsgI GTGCAG 1 cut(s) 1104
Bsh1285I CGRYCG 1 cut(s) 102
BshFI GGCC 1 cut(s) 1482
BsiEI CGRYCG 1 cut(s) 102
BsiHKAI GWGCWC 2 cut(s) 243, 1027
BsiHKCI CYCGRG 1 cut(s) 1965
BslI CCNNNNNNNGG 3 cut(s) 542, 1289, 1935
BsmAI GTCTC 3 cut(s) 259, 872, 1649
BsmBI CGTCTC 1 cut(s) 1649
BsnI GGCC 1 cut(s) 1482
BsoBI CYCGRG 1 cut(s) 1965
Bsp1286I GDGCHC 3 cut(s) 243, 1027, 1698
Bsp1720I GCTNAGC 1 cut(s) 1976
BspACI CCGC 5 cut(s) 51, 117, 169, 1558, 1726
BspANI GGCC 1 cut(s) 1482
BspCNI CTCAG 3 cut(s) 19, 157, 269
BspHI TCATGA 1 cut(s) 718
BspLI GGNNCC 3 cut(s) 107, 1179, 1656
BspMI ACCTGC 1 cut(s) 1181
BspPI GGATC 4 cut(s) 100, 113, 350, 1841
BspQI GCTCTTC 1 cut(s) 1015
BspTI CTTAAG 1 cut(s) 371
BsrDI GCAATG 3 cut(s) 1338, 1729, 1876
BsrI ACTGG 4 cut(s) 1077, 1294, 1648, 1813
BssSI CACGAG 1 cut(s) 1697
Bst2BI CACGAG 1 cut(s) 1697
Bst4CI ACNGT 4 cut(s) 74, 1173, 1584, 1786
Bst6I CTCTTC 3 cut(s) 397, 978, 1015
BstAFI CTTAAG 1 cut(s) 371
BstBAI YACGTR 2 cut(s) 94, 959
BstDEI CTNAG 5 cut(s) 6, 144, 256, 977, 1976
BstEII GGTNACC 1 cut(s) 1193
BstH2I RGCGCY 1 cut(s) 1957
BstHHI GCGC 1 cut(s) 1956
BstMAI GTCTC 3 cut(s) 259, 872, 1649
BstMCI CGRYCG 1 cut(s) 102
BstMWI GCNNNNNNNGC 2 cut(s) 673, 1693
BstNSI RCATGY 1 cut(s) 2044
BstPI GGTNACC 1 cut(s) 1193
BstSLI GKGCMC 1 cut(s) 1698
BstV1I GCAGC 2 cut(s) 1105, 2132
BstV2I GAAGAC 2 cut(s) 960, 1224
BstX2I RGATCY 2 cut(s) 105, 1846
BstYI RGATCY 2 cut(s) 105, 1846
BsuRI GGCC 1 cut(s) 1482
BtgZI GCGATG 1 cut(s) 984
BtrI CACGTC 1 cut(s) 587
BtsIMutI CAGTG 5 cut(s) 396, 504, 1178, 1287, 1782
BveI ACCTGC 1 cut(s) 1181
CaiI CAGNNNCTG 1 cut(s) 503
CciI TCATGA 1 cut(s) 718
CfoI GCGC 1 cut(s) 1956
Cfr13I GGNCC 1 cut(s) 1654
Csp6I GTAC 4 cut(s) 1487, 1615, 1774, 1895
CviAII CATG 9 cut(s) 383, 398, 719, 748, 755, 935, 1459, 1912, 2041
CviQI GTAC 4 cut(s) 1487, 1615, 1774, 1895
DdeI CTNAG 5 cut(s) 6, 144, 256, 977, 1976
DrdI GACNNNNNNGTC 1 cut(s) 1661
DseDI GACNNNNNNGTC 1 cut(s) 1661
Eam1104I CTCTTC 3 cut(s) 397, 978, 1015
EarI CTCTTC 3 cut(s) 397, 978, 1015
Ecl136II GAGCTC 1 cut(s) 1025
Eco24I GRGCYC 1 cut(s) 1027
Eco32I GATATC 1 cut(s) 1350
Eco47I GGWCC 1 cut(s) 1654
Eco47III AGCGCT 1 cut(s) 1955
Eco53kI GAGCTC 1 cut(s) 1025
Eco57I CTGAAG 7 cut(s) 342, 525, 617, 837, 882, 1293, 1827
Eco72I CACGTG 1 cut(s) 959
Eco88I CYCGRG 1 cut(s) 1965
Eco91I GGTNACC 1 cut(s) 1193
EcoICRI GAGCTC 1 cut(s) 1025
EcoO65I GGTNACC 1 cut(s) 1193
EcoRI GAATTC 1 cut(s) 1072
EcoRV GATATC 1 cut(s) 1350
EcoT38I GRGCYC 1 cut(s) 1027
Esp3I CGTCTC 1 cut(s) 1649
FaeI CATG 9 cut(s) 386, 401, 722, 751, 758, 938, 1462, 1915, 2044
FatI CATG 9 cut(s) 382, 397, 718, 747, 754, 934, 1458, 1911, 2040
FbaI TGATCA 1 cut(s) 1792
Fnu4HI GCNGC 3 cut(s) 170, 1119, 2121
FriOI GRGCYC 1 cut(s) 1027
Fsp4HI GCNGC 3 cut(s) 170, 1119, 2121
FspBI CTAG 2 cut(s) 159, 1661
GlaI GCGC 1 cut(s) 1955
GluI GCNGC 3 cut(s) 170, 1119, 2121
GsaI CCCAGC 1 cut(s) 1869
GsuI CTGGAG 1 cut(s) 1631
HaeII RGCGCY 1 cut(s) 1957
HaeIII GGCC 1 cut(s) 1482
HhaI GCGC 1 cut(s) 1956
Hin1II CATG 9 cut(s) 386, 401, 722, 751, 758, 938, 1462, 1915, 2044
Hin6I GCGC 1 cut(s) 1954
HinP1I GCGC 1 cut(s) 1954
HincII GTYRAC 3 cut(s) 70, 745, 1082
HindII GTYRAC 3 cut(s) 70, 745, 1082
HindIII AAGCTT 2 cut(s) 1856, 1978
HphI GGTGA 4 cut(s) 338, 1187, 1543, 2062
Hpy166II GTNNAC 5 cut(s) 70, 745, 1082, 1205, 1447
Hpy8I GTNNAC 5 cut(s) 70, 745, 1082, 1205, 1447
Hpy99I CGWCG 2 cut(s) 201, 1496
HpyAV CCTTC 8 cut(s) 317, 432, 535, 550, 1268, 1472, 1685, 1874
HpyCH4III ACNGT 4 cut(s) 74, 1173, 1584, 1786
HpyCH4IV ACGT 4 cut(s) 93, 586, 958, 1642
HpyCH4V TGCA 7 cut(s) 884, 1013, 1121, 1190, 1462, 1751, 1985
HpyF10VI GCNNNNNNNGC 2 cut(s) 673, 1693
HpyF3I CTNAG 5 cut(s) 6, 144, 256, 977, 1976
HpySE526I ACGT 4 cut(s) 93, 586, 958, 1642
Hsp92II CATG 9 cut(s) 386, 401, 722, 751, 758, 938, 1462, 1915, 2044
HspAI GCGC 1 cut(s) 1954
Ksp22I TGATCA 1 cut(s) 1792
LguI GCTCTTC 1 cut(s) 1015
LmnI GCTCC 5 cut(s) 77, 938, 1177, 1273, 1496
Lsp1109I GCAGC 2 cut(s) 1105, 2132
LweI GCATC 6 cut(s) 676, 893, 922, 1000, 1303, 1393
MaeI CTAG 2 cut(s) 159, 1661
MaeII ACGT 4 cut(s) 93, 586, 958, 1642
MflI RGATCY 2 cut(s) 105, 1846
MhlI GDGCHC 3 cut(s) 243, 1027, 1698
MluCI AATT 9 cut(s) 127, 286, 309, 921, 1072, 1111, 1184, 2000, 2075
MlyI GAGTC 1 cut(s) 1214
MmeI TCCRAC 2 cut(s) 1608, 1632
MroXI GAANNNNTTC 3 cut(s) 406, 930, 1677
MseI TTAA 3 cut(s) 372, 2003, 2031
MslI CAYNNNNRTG 4 cut(s) 1142, 1343, 1553, 1701
MspA1I CMGCKG 1 cut(s) 320
MspCI CTTAAG 1 cut(s) 371
MwoI GCNNNNNNNGC 2 cut(s) 673, 1693
NlaIII CATG 9 cut(s) 386, 401, 722, 751, 758, 938, 1462, 1915, 2044
NlaIV GGNNCC 3 cut(s) 107, 1179, 1656
NmuCI GTSAC 5 cut(s) 954, 1031, 1193, 1334, 2084
NspI RCATGY 1 cut(s) 2044
PagI TCATGA 1 cut(s) 718
PciI ACATGT 1 cut(s) 2040
PciSI GCTCTTC 1 cut(s) 1015
PcsI WCGNNNNNNNCGW 1 cut(s) 169
PdmI GAANNNNTTC 3 cut(s) 406, 930, 1677
PflMI CCANNNNNTGG 1 cut(s) 1289
PkrI GCNGC 3 cut(s) 171, 1120, 2122
Ple19I CGATCG 1 cut(s) 102
PleI GAGTC 1 cut(s) 1214
PmaCI CACGTG 1 cut(s) 959
PmlI CACGTG 1 cut(s) 959
PpsI GAGTC 1 cut(s) 1214
Ppu21I YACGTR 2 cut(s) 94, 959
PscI ACATGT 1 cut(s) 2040
PsiI TTATAA 1 cut(s) 453
Psp124BI GAGCTC 1 cut(s) 1027
PspCI CACGTG 1 cut(s) 959
PspEI GGTNACC 1 cut(s) 1193
PspFI CCCAGC 1 cut(s) 1865
PspN4I GGNNCC 3 cut(s) 107, 1179, 1656
PspPI GGNCC 1 cut(s) 1654
PstNI CAGNNNCTG 1 cut(s) 503
PsuI RGATCY 2 cut(s) 105, 1846
PvuI CGATCG 1 cut(s) 102
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 4 cut(s) 1488, 1616, 1775, 1896
RsaNI GTAC 4 cut(s) 1487, 1615, 1774, 1895
RseI CAYNNNNRTG 4 cut(s) 1142, 1343, 1553, 1701
SacI GAGCTC 1 cut(s) 1027
SapI GCTCTTC 1 cut(s) 1015
SaqAI TTAA 3 cut(s) 372, 2003, 2031
SatI GCNGC 3 cut(s) 170, 1119, 2121
Sau96I GGNCC 1 cut(s) 1654
SchI GAGTC 1 cut(s) 1214
SduI GDGCHC 3 cut(s) 243, 1027, 1698
SfaNI GCATC 6 cut(s) 676, 893, 922, 1000, 1303, 1393
SinI GGWCC 1 cut(s) 1654
SmiMI CAYNNNNRTG 4 cut(s) 1142, 1343, 1553, 1701
SmlI CTYRAG 3 cut(s) 371, 726, 1378
SmoI CTYRAG 3 cut(s) 371, 726, 1378
Sse9I AATT 9 cut(s) 127, 286, 309, 921, 1072, 1111, 1184, 2000, 2075
SsiI CCGC 5 cut(s) 51, 117, 169, 1558, 1726
SspMI CTAG 2 cut(s) 159, 1661
SstI GAGCTC 1 cut(s) 1027
TaaI ACNGT 4 cut(s) 74, 1173, 1584, 1786
TaiI ACGT 4 cut(s) 96, 589, 961, 1645
TaqI TCGA 6 cut(s) 46, 163, 181, 410, 707, 1027
TasI AATT 9 cut(s) 127, 286, 309, 921, 1072, 1111, 1184, 2000, 2075
TatI WGTACW 1 cut(s) 1773
TauI GCSGC 1 cut(s) 172
Tru1I TTAA 3 cut(s) 372, 2003, 2031
Tru9I TTAA 3 cut(s) 372, 2003, 2031
TscAI CASTG 5 cut(s) 396, 504, 1178, 1294, 1789
TseFI GTSAC 5 cut(s) 954, 1031, 1193, 1334, 2084
TseI GCWGC 2 cut(s) 1118, 2120
Tsp45I GTSAC 5 cut(s) 954, 1031, 1193, 1334, 2084
TspGWI ACGGA 2 cut(s) 98, 1509
TspRI CASTG 5 cut(s) 396, 504, 1178, 1294, 1789
Van91I CCANNNNNTGG 1 cut(s) 1289
Vha464I CTTAAG 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 1654
XapI RAATTY 3 cut(s) 1072, 1111, 1184
XceI RCATGY 1 cut(s) 2044
XmnI GAANNNNTTC 3 cut(s) 406, 930, 1677
XspI CTAG 2 cut(s) 159, 1661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.