FvH4_2g11770

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005063
Physical Location & Seq
Reverse (-)
10303128 .. 10310787
7660 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g11770.t1

Sequence Viewer

Length: 1773 bp
ATGGGTCAGTACAGACAGTCAGGGATGGAGAGGCTCGGATTACTACGTGGCAACGGCTCCGACGTGGCTATCGGGATCCGAACGCCGGGTCGGAGTCGCCGATCGGGTCGATCCCAAGTTAGAATCTCCCCCACCTCTGCTCTCCTTGTTGTCCTTGTCTCCTTAGTTCTCCTCCTTACTCTTCTCGCCTACTTTTACCTCTCCCTCACCAGCCCCTCCTCCTCAGGTGAAGATGCTCAGGAAGATGATTCAGACTTTCTCACAAATGTGACACGCACCCAGACTTCTAAGGTCCTTGGGTTTGGCAAGGGTTCGGTAACGCATGGCCGAGACTCTAGGTATTGGGATACAGATGATAGGAGAAGGGATGAAGACTATAATGAGGATACTCCCGTGGAAGTTAACAAGGAAGAGGCGTCTTTGAAATCTAATCATGAATATCTCGAAAAGGGACATGATGCTGATGATGAGTATGATGATGGGCTTGATTCTCAACAAACTCAGATGGATGAATATGATGATACTAATAGTGCTCTTCCTCATACTGGAATCAATTACGAAAATGTTTCCAGCAAGGTCCCCACTCACAACTCTCGCAACCTTGATCAAGTTGACACAAATTCTAGGCATCCTTCAAAAAGGTCGCGCTCAGATTCAAAAAGGAAAGCAAAGCGCCGTAAGTTTTCTGGTTCTTGTGAGATGAAGTTCTTAAACTCCACTGCACAGCTTATAGAGCCTTTAGAAAGTAGAAAGTTTGCGAGATTTTCCCTGCAGTATACTGAAATAGAAGACAAGCCCGAAGGACAAGAGCAATGGAAACCTAGATTTGCGGGGCATCAAAATTTGCAAGAACGGGAAAGTTCATTTTTGGCACGCGATCAAAAGATTAACTGTGGCTTTGTCAAAGGTCGTAAAGGATCTCCAAGCACTGGATTTGACTTGGCGGAAGATGACACAAATTATATCAGCAGATGCCATATTGCTGTTATCTCTTGCATCTTTGGGAATTCAGATCGCTTGAGAACGCCTTATGGTAAAACGGTCTCTCGTTTTTCAAGGAAGAATGTTTGCTTTGTTATGTTCATGGACAAGGTTACTTTGCGTACCCTTTCTTCAGAAGGGCAAACTCTAGATAGAATGGGTTTTATTGGTTTGTGGAAGATTGTTGTGGTGAAGAACCTTCCATATGCTGATATGCGGAGAGTGGGAAAGATACCAAAGCTTTTGCCTCACAGGCTTTTTCCTTCTGCAAGGTATTCAATTTGGTTGGACAGCAAGTTGCGTCTTCAACTTGACCCCTTACTCATATTGGAGTACTTTTTGTGGCGAAAAGGTCATGAGTATGCAATTTCGAATCACTACGATCGACACTGTGTATGGGAAGAGGTTGCCCAGAATAAAAAATTGAACAAGTATAACCATACCATCATAGATGAACAGTTTGCATTTTACAAGGATGATGGTCTTACAAGATTTAACGCATTGGATCCGAACAAGCTTCTTCCCAGCAATGTACCGGAAGGTTCTTTTATCATCAGGGCACACACTCCAATGTCAAATTTGTTTTCCTGTCTTTGGTTCAATGAGGTTGAGCGGTTTACTCCTCGGGATCAGCTAAGTTTTGCCTATACATACCAGAAATTAAGAAGGATGAATCCTGAAAAACCTTTTCATCTTAACATGTTCAAGGATTGTGAGAGAAGAGCCATAGCTAAGTTGTTTCGCCACAGGTCGGAAGAGAAGTCGAGTACTAGGCAAAGAGCTACCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

591

Amino Acids

68.26

Weight (kDa)

9.1

Isoelectric Point (pI)

51.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 257 - 576 5.1e-143 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 929
AccBSI CCGCTC 1 cut(s) 1594
AccI GTMKAC 1 cut(s) 776
AccII CGCG 2 cut(s) 646, 876
AciI CCGC 4 cut(s) 830, 944, 1198, 1594
AclWI GGATC 7 cut(s) 70, 83, 105, 925, 1481, 1494, 1617
AcoI YGGCCR 1 cut(s) 325
AcsI RAATTY 4 cut(s) 619, 841, 1006, 1558
AcuI CTGAAG 1 cut(s) 1098
AcyI GRCGYC 1 cut(s) 416
AfaI GTAC 5 cut(s) 11, 1105, 1316, 1515, 1750
AfiI CCNNNNNNNGG 5 cut(s) 85, 545, 929, 1575, 1732
AflIII ACRYGT 1 cut(s) 1680
AgsI TTSAA 9 cut(s) 424, 636, 657, 1056, 1260, 1289, 1408, 1582, 1687
AjiI CACGTC 1 cut(s) 64
AleI CACNNNNGTG 1 cut(s) 266
AluBI AGCT 6 cut(s) 727, 1222, 1498, 1615, 1712, 1763
AluI AGCT 6 cut(s) 727, 1222, 1498, 1615, 1712, 1763
Alw21I GWGCWC 1 cut(s) 535
Alw26I GTCTC 3 cut(s) 163, 324, 1048
AlwI GGATC 7 cut(s) 70, 83, 105, 925, 1481, 1494, 1617
Ama87I CYCGRG 1 cut(s) 1605
AoxI GGCC 1 cut(s) 325
ApoI RAATTY 4 cut(s) 619, 841, 1006, 1558
ArsI GACNNNNNNTTYG 2 cut(s) 73, 105
AspLEI GCGC 2 cut(s) 648, 675
AspS9I GGNCC 2 cut(s) 292, 577
AsuC2I CCSGG 1 cut(s) 87
AsuHPI GGTGA 3 cut(s) 199, 239, 1183
AsuII TTCGAA 1 cut(s) 1352
AvaI CYCGRG 1 cut(s) 1605
AvaII GGWCC 2 cut(s) 292, 577
AxyI CCTNAGG 1 cut(s) 223
BaeGI GKGCMC 1 cut(s) 1543
BaeI ACNNNNGTAYC 2 cut(s) 378, 411
BamHI GGATCC 2 cut(s) 75, 1486
BarI GAAGNNNNNNTAC 2 cut(s) 1096, 1128
BbsI GAAGAC 3 cut(s) 378, 795, 1277
Bbv12I GWGCWC 1 cut(s) 535
BccI CCATC 5 cut(s) 19, 473, 499, 1433, 1454
BceAI ACGGC 2 cut(s) 70, 660
BciVI GTATCC 2 cut(s) 340, 379
BclI TGATCA 1 cut(s) 604
BcnI CCSGG 1 cut(s) 87
BcoDI GTCTC 3 cut(s) 163, 324, 1048
BfaI CTAG 5 cut(s) 336, 624, 822, 1130, 1752
BfmI CTRYAG 1 cut(s) 770
BfoI RGCGCY 1 cut(s) 676
BfuI GTATCC 2 cut(s) 340, 379
BglI GCCNNNNNGGC 1 cut(s) 1234
BmcAI AGTACT 2 cut(s) 1316, 1750
Bme1390I CCNGG 1 cut(s) 87
Bme18I GGWCC 2 cut(s) 292, 577
BmeT110I CYCGRG 1 cut(s) 1605
BmgBI CACGTC 1 cut(s) 64
BmgT120I GGNCC 2 cut(s) 292, 577
BmiI GGNNCC 4 cut(s) 58, 77, 579, 1488
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 6 cut(s) 223, 448, 637, 844, 962, 1005
BpiI GAAGAC 3 cut(s) 378, 795, 1277
Bpu10I CCTNAGC 1 cut(s) 237
Bpu14I TTCGAA 1 cut(s) 1352
BpuEI CTTGAG 1 cut(s) 1039
BpuMI CCSGG 1 cut(s) 87
BsaAI YACGTR 1 cut(s) 47
BsaHI GRCGYC 1 cut(s) 416
BsaI GGTCTC 1 cut(s) 1048
BsaJI CCNNGG 3 cut(s) 295, 393, 1604
BsaWI WCCGGW 1 cut(s) 1516
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 545, 929, 1575, 1732
Bse1I ACTGG 2 cut(s) 550, 934
Bse21I CCTNAGG 1 cut(s) 223
Bse3DI GCAATG 2 cut(s) 818, 1516
BseDI CCNNGG 3 cut(s) 295, 393, 1604
BseGI GGATG 6 cut(s) 30, 373, 514, 628, 1462, 1656
BseLI CCNNNNNNNGG 5 cut(s) 85, 545, 929, 1575, 1732
BseMI GCAATG 2 cut(s) 818, 1516
BseMII CTCAG 4 cut(s) 237, 251, 515, 663
BseNI ACTGG 2 cut(s) 550, 934
BseRI GAGGAG 4 cut(s) 161, 208, 211, 1593
BseSI GKGCMC 1 cut(s) 1543
BseYI CCCAGC 1 cut(s) 1505
BsgI GTGCAG 1 cut(s) 705
Bsh1236I CGCG 2 cut(s) 646, 876
Bsh1285I CGRYCG 2 cut(s) 104, 1366
BshFI GGCC 1 cut(s) 327
BsiEI CGRYCG 2 cut(s) 104, 1366
BsiHKAI GWGCWC 1 cut(s) 535
BsiHKCI CYCGRG 1 cut(s) 1605
BsiSI CCGG 2 cut(s) 86, 1517
BslFI GGGAC 2 cut(s) 465, 563
BslI CCNNNNNNNGG 5 cut(s) 85, 545, 929, 1575, 1732
BsmAI GTCTC 3 cut(s) 163, 324, 1048
BsmFI GGGAC 2 cut(s) 465, 563
BsnI GGCC 1 cut(s) 327
Bso31I GGTCTC 1 cut(s) 1048
BsoBI CYCGRG 1 cut(s) 1605
Bsp119I TTCGAA 1 cut(s) 1352
Bsp1286I GDGCHC 2 cut(s) 535, 1543
BspACI CCGC 4 cut(s) 830, 944, 1198, 1594
BspANI GGCC 1 cut(s) 327
BspCNI CTCAG 4 cut(s) 236, 250, 514, 662
BspFNI CGCG 2 cut(s) 646, 876
BspHI TCATGA 2 cut(s) 433, 1336
BspLI GGNNCC 4 cut(s) 58, 77, 579, 1488
BspMAI CTGCAG 1 cut(s) 774
BspPI GGATC 7 cut(s) 70, 83, 105, 925, 1481, 1494, 1617
BspQI GCTCTTC 2 cut(s) 540, 1696
BspT104I TTCGAA 1 cut(s) 1352
BspTNI GGTCTC 1 cut(s) 1048
BsrBI CCGCTC 1 cut(s) 1594
BsrDI GCAATG 2 cut(s) 818, 1516
BsrI ACTGG 2 cut(s) 550, 934
BssECI CCNNGG 3 cut(s) 295, 393, 1604
BssNAI GTATAC 1 cut(s) 777
BssNI GRCGYC 1 cut(s) 416
BssT1I CCWWGG 1 cut(s) 295
Bst1107I GTATAC 1 cut(s) 777
Bst4CI ACNGT 5 cut(s) 18, 893, 1042, 1373, 1440
Bst6I CTCTTC 6 cut(s) 186, 405, 540, 1377, 1696, 1731
BstACI GRCGYC 1 cut(s) 416
BstBAI YACGTR 1 cut(s) 47
BstBI TTCGAA 1 cut(s) 1352
BstC8I GCNNGC 1 cut(s) 874
BstDEI CTNAG 8 cut(s) 163, 223, 237, 288, 501, 649, 1616, 1713
BstDSI CCRYGG 1 cut(s) 393
BstF5I GGATG 6 cut(s) 30, 373, 514, 628, 1462, 1656
BstFNI CGCG 2 cut(s) 646, 876
BstH2I RGCGCY 1 cut(s) 676
BstHHI GCGC 2 cut(s) 648, 675
BstMAI GTCTC 3 cut(s) 163, 324, 1048
BstMCI CGRYCG 2 cut(s) 104, 1366
BstMWI GCNNNNNNNGC 2 cut(s) 733, 1234
BstNSI RCATGY 1 cut(s) 1684
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 1 cut(s) 770
BstSLI GKGCMC 1 cut(s) 1543
BstUI CGCG 2 cut(s) 646, 876
BstV2I GAAGAC 3 cut(s) 378, 795, 1277
BstX2I RGATCY 3 cut(s) 75, 917, 1486
BstYI RGATCY 3 cut(s) 75, 917, 1486
BstZ17I GTATAC 1 cut(s) 777
Bsu36I CCTNAGG 1 cut(s) 223
BsuI GTATCC 2 cut(s) 340, 379
BsuRI GGCC 1 cut(s) 327
BtgI CCRYGG 1 cut(s) 393
BtrI CACGTC 1 cut(s) 64
BtsCI GGATG 6 cut(s) 30, 373, 514, 628, 1462, 1656
BtsI GCAGTG 1 cut(s) 717
BtsIMutI CAGTG 3 cut(s) 717, 927, 1369
Cac8I GCNNGC 1 cut(s) 874
CciI TCATGA 2 cut(s) 433, 1336
CfoI GCGC 2 cut(s) 648, 675
Cfr13I GGNCC 2 cut(s) 292, 577
CseI GACGC 2 cut(s) 405, 1271
Csp6I GTAC 5 cut(s) 10, 1104, 1315, 1514, 1749
CviAII CATG 6 cut(s) 323, 434, 455, 1084, 1337, 1681
CviQI GTAC 5 cut(s) 10, 1104, 1315, 1514, 1749
DdeI CTNAG 8 cut(s) 163, 223, 237, 288, 501, 649, 1616, 1713
EaeI YGGCCR 1 cut(s) 325
Eam1104I CTCTTC 6 cut(s) 186, 405, 540, 1377, 1696, 1731
EarI CTCTTC 6 cut(s) 186, 405, 540, 1377, 1696, 1731
EciI GGCGGA 1 cut(s) 959
Eco130I CCWWGG 1 cut(s) 295
Eco31I GGTCTC 1 cut(s) 1048
Eco47I GGWCC 2 cut(s) 292, 577
Eco57I CTGAAG 1 cut(s) 1098
Eco81I CCTNAGG 1 cut(s) 223
Eco88I CYCGRG 1 cut(s) 1605
EcoO109I RGGNCCY 2 cut(s) 292, 577
EcoRI GAATTC 1 cut(s) 1006
EcoT14I CCWWGG 1 cut(s) 295
ErhI CCWWGG 1 cut(s) 295
FaeI CATG 6 cut(s) 326, 437, 458, 1087, 1340, 1684
FaqI GGGAC 2 cut(s) 465, 563
FatI CATG 6 cut(s) 322, 433, 454, 1083, 1336, 1680
FauI CCCGC 1 cut(s) 823
FauNDI CATATG 1 cut(s) 1186
FbaI TGATCA 1 cut(s) 604
FblI GTMKAC 1 cut(s) 776
FokI GGATG 6 cut(s) 37, 380, 521, 615, 1469, 1663
FspBI CTAG 5 cut(s) 336, 624, 822, 1130, 1752
GlaI GCGC 2 cut(s) 647, 674
GsaI CCCAGC 1 cut(s) 1509
HaeII RGCGCY 1 cut(s) 676
HaeIII GGCC 1 cut(s) 327
HapII CCGG 2 cut(s) 86, 1517
HgaI GACGC 2 cut(s) 405, 1271
HhaI GCGC 2 cut(s) 648, 675
Hin1I GRCGYC 1 cut(s) 416
Hin1II CATG 6 cut(s) 326, 437, 458, 1087, 1340, 1684
Hin6I GCGC 2 cut(s) 646, 673
HinP1I GCGC 2 cut(s) 646, 673
HincII GTYRAC 2 cut(s) 403, 613
HindII GTYRAC 2 cut(s) 403, 613
HindIII AAGCTT 2 cut(s) 1220, 1496
HinfI GANTC 9 cut(s) 94, 123, 248, 332, 488, 549, 653, 1354, 1654
HpaI GTTAAC 1 cut(s) 403
HpaII CCGG 2 cut(s) 86, 1517
HphI GGTGA 3 cut(s) 199, 239, 1183
Hpy166II GTNNAC 4 cut(s) 403, 613, 777, 1599
Hpy188III TCNNGA 8 cut(s) 73, 239, 434, 443, 1130, 1337, 1607, 1658
Hpy8I GTNNAC 4 cut(s) 403, 613, 777, 1599
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 8 cut(s) 357, 642, 794, 1112, 1190, 1254, 1514, 1641
HpyCH4III ACNGT 5 cut(s) 18, 893, 1042, 1373, 1440
HpyCH4IV ACGT 2 cut(s) 46, 63
HpyCH4V TGCA 7 cut(s) 722, 772, 847, 996, 1250, 1346, 1445
HpyF10VI GCNNNNNNNGC 2 cut(s) 733, 1234
HpyF3I CTNAG 8 cut(s) 163, 223, 237, 288, 501, 649, 1616, 1713
HpySE526I ACGT 2 cut(s) 46, 63
Hsp92I GRCGYC 1 cut(s) 416
Hsp92II CATG 6 cut(s) 326, 437, 458, 1087, 1340, 1684
HspAI GCGC 2 cut(s) 646, 673
Ksp22I TGATCA 1 cut(s) 604
KspAI GTTAAC 1 cut(s) 403
LguI GCTCTTC 2 cut(s) 540, 1696
LmnI GCTCC 1 cut(s) 62
LweI GCATC 6 cut(s) 223, 448, 637, 844, 962, 1005
MaeI CTAG 5 cut(s) 336, 624, 822, 1130, 1752
MaeII ACGT 2 cut(s) 46, 63
MaeIII GTNAC 3 cut(s) 268, 316, 1093
MbiI CCGCTC 1 cut(s) 1594
MflI RGATCY 3 cut(s) 75, 917, 1486
MhlI GDGCHC 2 cut(s) 535, 1543
MlyI GAGTC 2 cut(s) 103, 326
MmeI TCCRAC 4 cut(s) 71, 84, 1248, 1713
MseI TTAA 6 cut(s) 402, 710, 888, 1476, 1643, 1677
MslI CAYNNNNRTG 3 cut(s) 266, 1341, 1550
MspI CCGG 2 cut(s) 86, 1517
MspR9I CCNGG 1 cut(s) 87
MvnI CGCG 2 cut(s) 646, 876
MwoI GCNNNNNNNGC 2 cut(s) 733, 1234
NciI CCSGG 1 cut(s) 87
NdeI CATATG 1 cut(s) 1186
NlaIII CATG 6 cut(s) 326, 437, 458, 1087, 1340, 1684
NlaIV GGNNCC 4 cut(s) 58, 77, 579, 1488
NmeAIII GCCGAG 1 cut(s) 353
NmuCI GTSAC 1 cut(s) 268
NspI RCATGY 1 cut(s) 1684
NspV TTCGAA 1 cut(s) 1352
OliI CACNNNNGTG 1 cut(s) 266
PagI TCATGA 2 cut(s) 433, 1336
PciI ACATGT 1 cut(s) 1680
PciSI GCTCTTC 2 cut(s) 540, 1696
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 97
PfeI GAWTC 7 cut(s) 123, 248, 488, 549, 653, 1354, 1654
PflMI CCANNNNNTGG 1 cut(s) 929
Ple19I CGATCG 2 cut(s) 104, 1366
PleI GAGTC 2 cut(s) 102, 326
PpsI GAGTC 2 cut(s) 102, 326
Ppu21I YACGTR 1 cut(s) 47
PpuMI RGGWCCY 2 cut(s) 292, 577
PscI ACATGT 1 cut(s) 1680
Psp5II RGGWCCY 2 cut(s) 292, 577
PspFI CCCAGC 1 cut(s) 1505
PspN4I GGNNCC 4 cut(s) 58, 77, 579, 1488
PspPI GGNCC 2 cut(s) 292, 577
PspPPI RGGWCCY 2 cut(s) 292, 577
PstI CTGCAG 1 cut(s) 774
PsuI RGATCY 3 cut(s) 75, 917, 1486
PvuI CGATCG 2 cut(s) 104, 1366
RsaI GTAC 5 cut(s) 11, 1105, 1316, 1515, 1750
RsaNI GTAC 5 cut(s) 10, 1104, 1315, 1514, 1749
RseI CAYNNNNRTG 3 cut(s) 266, 1341, 1550
SapI GCTCTTC 2 cut(s) 540, 1696
SaqAI TTAA 6 cut(s) 402, 710, 888, 1476, 1643, 1677
Sau96I GGNCC 2 cut(s) 292, 577
ScaI AGTACT 2 cut(s) 1316, 1750
SchI GAGTC 2 cut(s) 103, 326
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 2 cut(s) 535, 1543
SfaNI GCATC 6 cut(s) 223, 448, 637, 844, 962, 1005
SfcI CTRYAG 1 cut(s) 770
SfuI TTCGAA 1 cut(s) 1352
SinI GGWCC 2 cut(s) 292, 577
SmiMI CAYNNNNRTG 3 cut(s) 266, 1341, 1550
SmlI CTYRAG 1 cut(s) 1018
SmoI CTYRAG 1 cut(s) 1018
SsiI CCGC 4 cut(s) 830, 944, 1198, 1594
SspMI CTAG 5 cut(s) 336, 624, 822, 1130, 1752
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 5 cut(s) 18, 893, 1042, 1373, 1440
TaiI ACGT 2 cut(s) 49, 66
TaqI TCGA 5 cut(s) 109, 444, 1352, 1366, 1745
TatI WGTACW 3 cut(s) 9, 1314, 1748
TfiI GAWTC 7 cut(s) 123, 248, 488, 549, 653, 1354, 1654
Tru1I TTAA 6 cut(s) 402, 710, 888, 1476, 1643, 1677
Tru9I TTAA 6 cut(s) 402, 710, 888, 1476, 1643, 1677
TscAI CASTG 3 cut(s) 724, 934, 1376
TseFI GTSAC 1 cut(s) 268
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 9 cut(s) 384, 450, 525, 716, 852, 1072, 1449, 1661, 1667
TspRI CASTG 3 cut(s) 724, 934, 1376
Van91I CCANNNNNTGG 1 cut(s) 929
VpaK11BI GGWCC 2 cut(s) 292, 577
XapI RAATTY 4 cut(s) 619, 841, 1006, 1558
XbaI TCTAGA 1 cut(s) 1129
XceI RCATGY 1 cut(s) 1684
XmiI GTMKAC 1 cut(s) 776
XspI CTAG 5 cut(s) 336, 624, 822, 1130, 1752
ZrmI AGTACT 2 cut(s) 1316, 1750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.