Rh6BG184400

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0005063
Physical Location & Seq
Reverse (-)
33879020 .. 33888164
9145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG184400.1

Sequence Viewer

Length: 1590 bp
ATGGCTCAGTACAGACAGTCAGGGATGGAGACTAGGTTGGGATTACGTGGCGGCAACGGCGGGGATGGACCAGATCACGTCGCTATCGGGATCCGAACGGGTCTACATAAACCGGGTCGGACTCGCCGATCGGGTCGATCCCCACCTAGAATCTCTACTTGTGCTTTAGTTCTCGTTCTCTCCTTGGTTCTCCTTTTTACTGTTGCCGCCTACTTCTACCTCTCCCTCAGCACCACCACACCCACAGGTGCGGAAATAAATAGTAATCGTGCTCAGGATGATGATTCGAGGAATGATTCTGACTTTCTCACGAATGTGACACGCACCGAGACTTCTAAAGTGCTTAAGTTTGGGAAGGGTTCAGTGACGCATGGCCGGGATTCTAGGTATTGGGATAAAGATGATAGGAGAAGGGATGAAGACTATAATGAGGATGTGGTGGAGCATGGTGGCGCAGGAGTTGATGATGAATCTACTCGTGCGACTCAAGTTAAGGACAGTAAGAACAAGTCCGAGAATGATGACTTTCTTAACAGTTCCAATAAGAAAGCTGGATTGTACAATGAAGCCGGGCGCAAGGAGTTGAAAATATACGAAGCAGAGTATGAGGCATCTTTGAAGAACAATCGACTGTCTAATGATGTATATTTGGAAACGCGAAATGACGTTATTGATGCTGATGATGAGTATGATGATGGGATCGATTCTCGTGAAACTCGTATGGAAGAATATGATGATACTAGACATCAAAATGGTGACCATTTTAGTGAAGAAAAATTACATGATGAAGGTGGTAGAGAGTCCATTGATCTTCCTGATGCTGGAATCAATTATGAAAATGTCTCCAAGAAGGTTGATAAACTCTCCACCAATTCATTTAATGAGCACTCTGCCCAGTACTCTCGGAACCTTGATGAAGTTAACACAAATTCTAGGCATGTTGGTGTAATTTCCGGTCATTCTTCAAGAAAATCGCACTCAGAATCAAAAAGAAAAGCAAAGCGCCACAAATTTTCTGGTTCCTCTTGTGAGATGAAGTTCTTAAACTCCACTGCACAGCTTATAGAGCCTTTAGAAAGTAGAAAATTTGCGAGATTTTCCCTGCAGTATACTGAAATAGAAGACAAGCCCGAAGGAGAAGAGCAATGGGAACCTAGATTTGCAGGACATCAGACTTTGCAAGAACGGGAAAATTCTTTTTTGGCACGTGATCAAAAAATTAACTGTGGCTTTGTTAAAGGTCCTAAAGGATCTCCAAGCACTGGATTTGACTTGTCAGAGGATGATGCAAATTATATCAGTAGATGCCACATTGCTGTTATCTCTTGTATCTTTGGAAATTCGGATCGCTTGAGAACACCTTACGGTAAAATGGTCTCTCGTTTGTCAAGGAAGAATGTTTGCTTTGTTATGTTCATCGACGAGGTTACGTTGCGTACGCTTTCTTCAGAAGGGCAAACGCTAGATAGCATTGGTTTTATTGGTTTGTGGAAGATTGTTGTCGTGAAGAACCTACCATATACTGATATGCGGAGAGTGGGAAAGATACCAAAGCTTTTGCCTCATAGGCTTTTTCCTTCTGCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

529

Amino Acids

59.76

Weight (kDa)

6.35

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 368 - 529 1.4e-61 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1262
AccI GTMKAC 2 cut(s) 103, 1109
AccII CGCG 1 cut(s) 658
AciI CCGC 5 cut(s) 51, 60, 207, 251, 1531
AclWI GGATC 6 cut(s) 85, 98, 132, 707, 1258, 1353
AcoI YGGCCR 1 cut(s) 373
AcsI RAATTY 5 cut(s) 928, 1010, 1085, 1192, 1339
AcuI CTGAAG 1 cut(s) 1431
AcvI CACGTG 1 cut(s) 1208
AfaI GTAC 4 cut(s) 11, 560, 899, 1438
AfiI CCNNNNNNNGG 2 cut(s) 821, 1262
AflII CTTAAG 1 cut(s) 344
AgsI TTSAA 3 cut(s) 586, 619, 966
AjiI CACGTC 1 cut(s) 79
AleI CACNNNNGTG 1 cut(s) 314
AluBI AGCT 3 cut(s) 551, 1060, 1555
AluI AGCT 3 cut(s) 551, 1060, 1555
Alw21I GWGCWC 2 cut(s) 274, 888
Alw26I GTCTC 4 cut(s) 23, 323, 847, 1381
AlwI GGATC 6 cut(s) 85, 98, 132, 707, 1258, 1353
AoxI GGCC 1 cut(s) 373
ApoI RAATTY 5 cut(s) 928, 1010, 1085, 1192, 1339
AspLEI GCGC 3 cut(s) 455, 576, 1005
AspS9I GGNCC 2 cut(s) 68, 1241
AsuC2I CCSGG 3 cut(s) 114, 377, 571
AsuHPI GGTGA 1 cut(s) 767
AvaII GGWCC 2 cut(s) 68, 1241
BamHI GGATCC 1 cut(s) 90
BarI GAAGNNNNNNTAC 4 cut(s) 762, 794, 1429, 1461
BauI CACGAG 2 cut(s) 477, 708
BbrPI CACGTG 1 cut(s) 1208
BbsI GAAGAC 2 cut(s) 426, 1128
Bbv12I GWGCWC 2 cut(s) 274, 888
BbvCI CCTCAGC 1 cut(s) 227
BccI CCATC 3 cut(s) 19, 59, 689
BceAI ACGGC 1 cut(s) 73
BclI TGATCA 1 cut(s) 1210
BcnI CCSGG 3 cut(s) 114, 377, 571
BcoDI GTCTC 4 cut(s) 23, 323, 847, 1381
BfaI CTAG 7 cut(s) 33, 147, 384, 741, 933, 1155, 1463
BfmI CTRYAG 1 cut(s) 1103
BfoI RGCGCY 1 cut(s) 1006
BfrI CTTAAG 1 cut(s) 344
BglI GCCNNNNNGGC 1 cut(s) 1567
BisI GCNGC 2 cut(s) 52, 207
BlsI GCNGC 2 cut(s) 53, 208
BmcAI AGTACT 1 cut(s) 899
Bme1390I CCNGG 3 cut(s) 114, 377, 571
Bme18I GGWCC 2 cut(s) 68, 1241
BmgBI CACGTC 1 cut(s) 79
BmgT120I GGNCC 2 cut(s) 68, 1241
BmiI GGNNCC 4 cut(s) 92, 908, 1021, 1152
BmrFI CCNGG 3 cut(s) 114, 377, 571
BmrI ACTGGG 1 cut(s) 889
BmsI GCATC 5 cut(s) 620, 664, 808, 1276, 1295
BmuI ACTGGG 1 cut(s) 889
BpiI GAAGAC 2 cut(s) 426, 1128
Bpu10I CCTNAGC 2 cut(s) 227, 273
BpuEI CTTGAG 2 cut(s) 471, 1372
BpuMI CCSGG 3 cut(s) 114, 377, 571
Bsa29I ATCGAT 1 cut(s) 702
BsaAI YACGTR 2 cut(s) 47, 1208
BsaI GGTCTC 1 cut(s) 1381
BsaJI CCNNGG 1 cut(s) 183
BsaWI WCCGGW 1 cut(s) 953
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 2 cut(s) 821, 1262
Bse1I ACTGG 2 cut(s) 895, 1267
Bse3DI GCAATG 2 cut(s) 1151, 1311
BseCI ATCGAT 1 cut(s) 702
BseDI CCNNGG 1 cut(s) 183
BseGI GGATG 6 cut(s) 30, 70, 283, 421, 439, 1288
BseLI CCNNNNNNNGG 2 cut(s) 821, 1262
BseMI GCAATG 2 cut(s) 1151, 1311
BseMII CTCAG 4 cut(s) 20, 241, 287, 993
BseNI ACTGG 2 cut(s) 895, 1267
BsgI GTGCAG 1 cut(s) 1038
Bsh1236I CGCG 1 cut(s) 658
Bsh1285I CGRYCG 1 cut(s) 131
BshFI GGCC 1 cut(s) 375
BshVI ATCGAT 1 cut(s) 702
BsiEI CGRYCG 1 cut(s) 131
BsiHKAI GWGCWC 2 cut(s) 274, 888
BsiSI CCGG 4 cut(s) 113, 376, 570, 954
BsiWI CGTACG 1 cut(s) 1436
BslI CCNNNNNNNGG 2 cut(s) 821, 1262
BsmAI GTCTC 4 cut(s) 23, 323, 847, 1381
BsnI GGCC 1 cut(s) 375
Bso31I GGTCTC 1 cut(s) 1381
Bsp1286I GDGCHC 2 cut(s) 274, 888
Bsp1407I TGTACA 1 cut(s) 558
Bsp143I GATC 9 cut(s) 73, 90, 128, 137, 699, 808, 1210, 1250, 1345
BspACI CCGC 5 cut(s) 51, 60, 207, 251, 1531
BspANI GGCC 1 cut(s) 375
BspCNI CTCAG 4 cut(s) 19, 240, 286, 992
BspDI ATCGAT 1 cut(s) 702
BspFNI CGCG 1 cut(s) 658
BspLI GGNNCC 4 cut(s) 92, 908, 1021, 1152
BspMAI CTGCAG 1 cut(s) 1107
BspPI GGATC 6 cut(s) 85, 98, 132, 707, 1258, 1353
BspQI GCTCTTC 1 cut(s) 1134
BspTI CTTAAG 1 cut(s) 344
BspTNI GGTCTC 1 cut(s) 1381
BsrDI GCAATG 2 cut(s) 1151, 1311
BsrGI TGTACA 1 cut(s) 558
BsrI ACTGG 2 cut(s) 895, 1267
BssECI CCNNGG 1 cut(s) 183
BssMI GATC 9 cut(s) 73, 90, 128, 137, 699, 808, 1210, 1250, 1345
BssNAI GTATAC 1 cut(s) 1110
BssSI CACGAG 2 cut(s) 477, 708
BssT1I CCWWGG 1 cut(s) 183
Bst1107I GTATAC 1 cut(s) 1110
Bst2BI CACGAG 2 cut(s) 477, 708
Bst4CI ACNGT 7 cut(s) 18, 202, 500, 536, 633, 1226, 1367
Bst6I CTCTTC 1 cut(s) 1134
BstAFI CTTAAG 1 cut(s) 344
BstAUI TGTACA 1 cut(s) 558
BstBAI YACGTR 2 cut(s) 47, 1208
BstDEI CTNAG 4 cut(s) 6, 227, 273, 979
BstEII GGTNACC 1 cut(s) 755
BstF5I GGATG 6 cut(s) 30, 70, 283, 421, 439, 1288
BstFNI CGCG 1 cut(s) 658
BstH2I RGCGCY 1 cut(s) 1006
BstHHI GCGC 3 cut(s) 455, 576, 1005
BstKTI GATC 9 cut(s) 76, 93, 131, 140, 702, 811, 1213, 1253, 1348
BstMAI GTCTC 4 cut(s) 23, 323, 847, 1381
BstMBI GATC 9 cut(s) 73, 90, 128, 137, 699, 808, 1210, 1250, 1345
BstMCI CGRYCG 1 cut(s) 131
BstMWI GCNNNNNNNGC 3 cut(s) 57, 1066, 1567
BstNSI RCATGY 1 cut(s) 941
BstPI GGTNACC 1 cut(s) 755
BstSCI CCNGG 3 cut(s) 112, 375, 569
BstSFI CTRYAG 1 cut(s) 1103
BstUI CGCG 1 cut(s) 658
BstV2I GAAGAC 2 cut(s) 426, 1128
BstX2I RGATCY 2 cut(s) 90, 1250
BstYI RGATCY 2 cut(s) 90, 1250
BstZ17I GTATAC 1 cut(s) 1110
Bsu15I ATCGAT 1 cut(s) 702
BsuRI GGCC 1 cut(s) 375
BsuTUI ATCGAT 1 cut(s) 702
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 6 cut(s) 30, 70, 283, 421, 439, 1288
BtsI GCAGTG 1 cut(s) 1050
BtsIMutI CAGTG 3 cut(s) 369, 1050, 1260
CfoI GCGC 3 cut(s) 455, 576, 1005
Cfr13I GGNCC 2 cut(s) 68, 1241
ClaI ATCGAT 1 cut(s) 702
CseI GACGC 1 cut(s) 376
Csp6I GTAC 4 cut(s) 10, 559, 898, 1437
CviAII CATG 4 cut(s) 371, 446, 782, 938
CviQI GTAC 4 cut(s) 10, 559, 898, 1437
DdeI CTNAG 4 cut(s) 6, 227, 273, 979
DpnI GATC 9 cut(s) 75, 92, 130, 139, 701, 810, 1212, 1252, 1347
DpnII GATC 9 cut(s) 73, 90, 128, 137, 699, 808, 1210, 1250, 1345
EaeI YGGCCR 1 cut(s) 373
Eam1104I CTCTTC 1 cut(s) 1134
EarI CTCTTC 1 cut(s) 1134
Eco130I CCWWGG 1 cut(s) 183
Eco31I GGTCTC 1 cut(s) 1381
Eco47I GGWCC 2 cut(s) 68, 1241
Eco57I CTGAAG 1 cut(s) 1431
Eco72I CACGTG 1 cut(s) 1208
Eco91I GGTNACC 1 cut(s) 755
EcoO109I RGGNCCY 1 cut(s) 1241
EcoO65I GGTNACC 1 cut(s) 755
EcoT14I CCWWGG 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 4 cut(s) 374, 449, 785, 941
FatI CATG 4 cut(s) 370, 445, 781, 937
FauI CCCGC 1 cut(s) 53
FbaI TGATCA 1 cut(s) 1210
FblI GTMKAC 2 cut(s) 103, 1109
Fnu4HI GCNGC 2 cut(s) 52, 207
FokI GGATG 6 cut(s) 37, 77, 290, 428, 446, 1295
Fsp4HI GCNGC 2 cut(s) 52, 207
FspBI CTAG 7 cut(s) 33, 147, 384, 741, 933, 1155, 1463
GlaI GCGC 3 cut(s) 454, 575, 1004
GluI GCNGC 2 cut(s) 52, 207
HaeII RGCGCY 1 cut(s) 1006
HaeIII GGCC 1 cut(s) 375
HapII CCGG 4 cut(s) 113, 376, 570, 954
HgaI GACGC 1 cut(s) 376
HhaI GCGC 3 cut(s) 455, 576, 1005
Hin1II CATG 4 cut(s) 374, 449, 785, 941
Hin6I GCGC 3 cut(s) 453, 574, 1003
HinP1I GCGC 3 cut(s) 453, 574, 1003
HincII GTYRAC 1 cut(s) 922
HindII GTYRAC 1 cut(s) 922
HindIII AAGCTT 1 cut(s) 1553
HpaI GTTAAC 1 cut(s) 922
HpaII CCGG 4 cut(s) 113, 376, 570, 954
HphI GGTGA 1 cut(s) 767
Hpy166II GTNNAC 3 cut(s) 104, 922, 1110
Hpy188III TCNNGA 7 cut(s) 88, 275, 310, 710, 815, 966, 1504
Hpy8I GTNNAC 3 cut(s) 104, 922, 1110
Hpy99I CGWCG 2 cut(s) 83, 1424
HpyAV CCTTC 7 cut(s) 349, 405, 782, 844, 1127, 1445, 1587
HpyCH4III ACNGT 7 cut(s) 18, 202, 500, 536, 633, 1226, 1367
HpyCH4IV ACGT 5 cut(s) 46, 78, 666, 1207, 1430
HpyCH4V TGCA 6 cut(s) 1055, 1105, 1163, 1180, 1289, 1583
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 1066, 1567
HpyF3I CTNAG 4 cut(s) 6, 227, 273, 979
HpySE526I ACGT 5 cut(s) 46, 78, 666, 1207, 1430
Hsp92II CATG 4 cut(s) 374, 449, 785, 941
HspAI GCGC 3 cut(s) 453, 574, 1003
Ksp22I TGATCA 1 cut(s) 1210
KspAI GTTAAC 1 cut(s) 922
Kzo9I GATC 9 cut(s) 73, 90, 128, 137, 699, 808, 1210, 1250, 1345
LguI GCTCTTC 1 cut(s) 1134
LmnI GCTCC 1 cut(s) 442
LweI GCATC 5 cut(s) 620, 664, 808, 1276, 1295
MaeI CTAG 7 cut(s) 33, 147, 384, 741, 933, 1155, 1463
MaeII ACGT 5 cut(s) 46, 78, 666, 1207, 1430
MaeIII GTNAC 4 cut(s) 316, 364, 755, 1426
MalI GATC 9 cut(s) 75, 92, 130, 139, 701, 810, 1212, 1252, 1347
MboI GATC 9 cut(s) 73, 90, 128, 137, 699, 808, 1210, 1250, 1345
MflI RGATCY 2 cut(s) 90, 1250
MhlI GDGCHC 2 cut(s) 274, 888
MlyI GAGTC 3 cut(s) 115, 478, 809
MmeI TCCRAC 1 cut(s) 98
MnlI CCTC 9 cut(s) 230, 236, 282, 424, 601, 1033, 1273, 1417, 1572
MseI TTAA 8 cut(s) 345, 492, 531, 879, 921, 1043, 1221, 1236
MslI CAYNNNNRTG 4 cut(s) 314, 750, 765, 942
MspCI CTTAAG 1 cut(s) 344
MspI CCGG 4 cut(s) 113, 376, 570, 954
MspR9I CCNGG 3 cut(s) 114, 377, 571
MvnI CGCG 1 cut(s) 658
MwoI GCNNNNNNNGC 3 cut(s) 57, 1066, 1567
NciI CCSGG 3 cut(s) 114, 377, 571
NdeII GATC 9 cut(s) 73, 90, 128, 137, 699, 808, 1210, 1250, 1345
NlaIII CATG 4 cut(s) 374, 449, 785, 941
NlaIV GGNNCC 4 cut(s) 92, 908, 1021, 1152
NmuCI GTSAC 3 cut(s) 316, 364, 755
NspI RCATGY 1 cut(s) 941
OliI CACNNNNGTG 1 cut(s) 314
PciSI GCTCTTC 1 cut(s) 1134
PcsI WCGNNNNNNNCGW 2 cut(s) 124, 715
PfeI GAWTC 8 cut(s) 150, 284, 296, 380, 470, 704, 825, 983
Pfl23II CGTACG 1 cut(s) 1436
PflMI CCANNNNNTGG 1 cut(s) 1262
PkrI GCNGC 2 cut(s) 53, 208
Ple19I CGATCG 1 cut(s) 131
PleI GAGTC 3 cut(s) 115, 478, 808
PmaCI CACGTG 1 cut(s) 1208
PmlI CACGTG 1 cut(s) 1208
PpsI GAGTC 3 cut(s) 115, 478, 808
Ppu21I YACGTR 2 cut(s) 47, 1208
PpuMI RGGWCCY 1 cut(s) 1241
Psp5II RGGWCCY 1 cut(s) 1241
PspCI CACGTG 1 cut(s) 1208
PspEI GGTNACC 1 cut(s) 755
PspLI CGTACG 1 cut(s) 1436
PspN4I GGNNCC 4 cut(s) 92, 908, 1021, 1152
PspPI GGNCC 2 cut(s) 68, 1241
PspPPI RGGWCCY 1 cut(s) 1241
PstI CTGCAG 1 cut(s) 1107
PsuI RGATCY 2 cut(s) 90, 1250
PvuI CGATCG 1 cut(s) 131
RsaI GTAC 4 cut(s) 11, 560, 899, 1438
RsaNI GTAC 4 cut(s) 10, 559, 898, 1437
RseI CAYNNNNRTG 4 cut(s) 314, 750, 765, 942
SapI GCTCTTC 1 cut(s) 1134
SaqAI TTAA 8 cut(s) 345, 492, 531, 879, 921, 1043, 1221, 1236
SatI GCNGC 2 cut(s) 52, 207
Sau3AI GATC 9 cut(s) 73, 90, 128, 137, 699, 808, 1210, 1250, 1345
Sau96I GGNCC 2 cut(s) 68, 1241
ScaI AGTACT 1 cut(s) 899
SchI GAGTC 3 cut(s) 115, 478, 809
ScrFI CCNGG 3 cut(s) 114, 377, 571
SduI GDGCHC 2 cut(s) 274, 888
SfaNI GCATC 5 cut(s) 620, 664, 808, 1276, 1295
SfcI CTRYAG 1 cut(s) 1103
SinI GGWCC 2 cut(s) 68, 1241
SmiMI CAYNNNNRTG 4 cut(s) 314, 750, 765, 942
SmlI CTYRAG 3 cut(s) 344, 486, 1351
SmoI CTYRAG 3 cut(s) 344, 486, 1351
SsiI CCGC 5 cut(s) 51, 60, 207, 251, 1531
SspMI CTAG 7 cut(s) 33, 147, 384, 741, 933, 1155, 1463
StyD4I CCNGG 3 cut(s) 112, 375, 569
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 7 cut(s) 18, 202, 500, 536, 633, 1226, 1367
TaiI ACGT 5 cut(s) 49, 81, 669, 1210, 1433
TaqI TCGA 5 cut(s) 136, 287, 628, 702, 1419
TatI WGTACW 3 cut(s) 9, 558, 897
TauI GCSGC 2 cut(s) 54, 209
TfiI GAWTC 8 cut(s) 150, 284, 296, 380, 470, 704, 825, 983
Tru1I TTAA 8 cut(s) 345, 492, 531, 879, 921, 1043, 1221, 1236
Tru9I TTAA 8 cut(s) 345, 492, 531, 879, 921, 1043, 1221, 1236
TscAI CASTG 3 cut(s) 369, 1057, 1267
TseFI GTSAC 3 cut(s) 316, 364, 755
Tsp45I GTSAC 3 cut(s) 316, 364, 755
TspDTI ATGAA 9 cut(s) 432, 483, 579, 801, 849, 864, 930, 1049, 1405
TspRI CASTG 3 cut(s) 369, 1057, 1267
Van91I CCANNNNNTGG 1 cut(s) 1262
Vha464I CTTAAG 1 cut(s) 344
VpaK11BI GGWCC 2 cut(s) 68, 1241
XapI RAATTY 5 cut(s) 928, 1010, 1085, 1192, 1339
XceI RCATGY 1 cut(s) 941
XcmI CCANNNNNNNNNTGG 1 cut(s) 1013
XmiI GTMKAC 2 cut(s) 103, 1109
XspI CTAG 7 cut(s) 33, 147, 384, 741, 933, 1155, 1463
ZrmI AGTACT 1 cut(s) 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.