Rmu_sc0013301.1_g000001

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0013301.1
OG0005063
Physical Location & Seq
Forward (+)
1 .. 3294
3294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0013301.1_g000001.1.cds

Sequence Viewer

Length: 732 bp
gtctctcgtttgtcaaggaagaatgtttgctttgttatgttcatcgacgaggttacgttgcgtaccctttcttcagaagggcaaacactagatagcattggttttattggtttgtggaagattgttgtggtgaagaacctaccatatactgatatgcggagagtgggaaagataccaaagcttttgcctcataggctttttccttctgcaaggtattcaatctggttggacagcaagttgcgtcttcaacttgaccccttactcatattggaatattttttgtggcgaaaaggtcatgagtatgcaatttcgaatcactatgatcgacactgtgtatgggaagaggttgcccagaataaaaaattgaacaagtataatcataccatcatagaccaacaatttgcattttaccaggacgatggtctcacaagattcaacgcattggatccgaacaagcttctccccagcaatgtgccagaaggttcttttatcatccgagcacacactccaatgtcaaatttgttttcctgtctttggttcaatgaggttgagcggtttactcctcgggatcagctaagttttgcctatacataccagaaattaagaaggatgaatcctgaaaaaccttttcgtcttcatatgttcaaggattgtgagagaagagccattgctaagttgtttcgccacaggtcacaagagaagtcgagtactcggcaaagagctactgagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

243

Amino Acids

28.99

Weight (kDa)

9.78

Isoelectric Point (pI)

50.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 553
AciI CCGC 2 cut(s) 157, 553
AclWI GGATC 3 cut(s) 440, 453, 576
AcsI RAATTY 1 cut(s) 517
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 2 cut(s) 64, 709
AfiI CCNNNNNNNGG 1 cut(s) 534
AgsI TTSAA 6 cut(s) 219, 248, 367, 436, 541, 646
AjnI CCWGG 1 cut(s) 411
AluBI AGCT 4 cut(s) 181, 457, 574, 722
AluI AGCT 4 cut(s) 181, 457, 574, 722
Alw21I GWGCWC 1 cut(s) 502
Alw26I GTCTC 2 cut(s) 7, 428
AlwI GGATC 3 cut(s) 440, 453, 576
Ama87I CYCGRG 1 cut(s) 564
ApoI RAATTY 1 cut(s) 517
ArsI GACNNNNNNTTYG 2 cut(s) 383, 415
AsuHPI GGTGA 1 cut(s) 142
AsuII TTCGAA 1 cut(s) 311
AvaI CYCGRG 1 cut(s) 564
BamHI GGATCC 1 cut(s) 445
BarI GAAGNNNNNNTAC 2 cut(s) 55, 87
BbsI GAAGAC 2 cut(s) 236, 626
Bbv12I GWGCWC 1 cut(s) 502
BccI CCATC 2 cut(s) 392, 413
BciT130I CCWGG 1 cut(s) 413
BcoDI GTCTC 2 cut(s) 7, 428
BfaI CTAG 1 cut(s) 89
BglI GCCNNNNNGGC 1 cut(s) 193
BmcAI AGTACT 1 cut(s) 709
Bme1390I CCNGG 1 cut(s) 413
BmeT110I CYCGRG 1 cut(s) 564
BmiI GGNNCC 1 cut(s) 447
BmrFI CCNGG 1 cut(s) 413
BoxI GACNNNNGTC 1 cut(s) 420
BpiI GAAGAC 2 cut(s) 236, 626
Bpu14I TTCGAA 1 cut(s) 311
BsaI GGTCTC 1 cut(s) 428
BsaJI CCNNGG 1 cut(s) 563
Bsc4I CCNNNNNNNGG 1 cut(s) 534
Bse3DI GCAATG 2 cut(s) 475, 666
BseBI CCWGG 1 cut(s) 413
BseDI CCNNGG 1 cut(s) 563
BseGI GGATG 2 cut(s) 492, 615
BseLI CCNNNNNNNGG 1 cut(s) 534
BseMI GCAATG 2 cut(s) 475, 666
BseMII CTCAG 1 cut(s) 717
BseRI GAGGAG 1 cut(s) 552
BseYI CCCAGC 1 cut(s) 464
BsiHKAI GWGCWC 1 cut(s) 502
BsiHKCI CYCGRG 1 cut(s) 564
BslI CCNNNNNNNGG 1 cut(s) 534
BsmAI GTCTC 2 cut(s) 7, 428
Bso31I GGTCTC 1 cut(s) 428
BsoBI CYCGRG 1 cut(s) 564
Bsp119I TTCGAA 1 cut(s) 311
Bsp1286I GDGCHC 1 cut(s) 502
Bsp143I GATC 3 cut(s) 322, 445, 568
BspACI CCGC 2 cut(s) 157, 553
BspCNI CTCAG 1 cut(s) 718
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 1 cut(s) 447
BspPI GGATC 3 cut(s) 440, 453, 576
BspQI GCTCTTC 1 cut(s) 655
BspT104I TTCGAA 1 cut(s) 311
BspTNI GGTCTC 1 cut(s) 428
BsrBI CCGCTC 1 cut(s) 553
BsrDI GCAATG 2 cut(s) 475, 666
BssECI CCNNGG 1 cut(s) 563
BssMI GATC 3 cut(s) 322, 445, 568
Bst2UI CCWGG 1 cut(s) 413
Bst4CI ACNGT 1 cut(s) 332
Bst6I CTCTTC 2 cut(s) 336, 655
BstBI TTCGAA 1 cut(s) 311
BstDEI CTNAG 3 cut(s) 575, 672, 726
BstF5I GGATG 2 cut(s) 492, 615
BstKTI GATC 3 cut(s) 325, 448, 571
BstMAI GTCTC 2 cut(s) 7, 428
BstMBI GATC 3 cut(s) 322, 445, 568
BstMWI GCNNNNNNNGC 1 cut(s) 193
BstNI CCWGG 1 cut(s) 413
BstPAI GACNNNNGTC 1 cut(s) 420
BstSCI CCNGG 1 cut(s) 411
BstV2I GAAGAC 2 cut(s) 236, 626
BstX2I RGATCY 1 cut(s) 445
BstXI CCANNNNNNTGG 1 cut(s) 419
BstYI RGATCY 1 cut(s) 445
BtsCI GGATG 2 cut(s) 492, 615
BtsIMutI CAGTG 1 cut(s) 328
CciI TCATGA 1 cut(s) 295
CseI GACGC 1 cut(s) 230
Csp6I GTAC 2 cut(s) 63, 708
CviAII CATG 1 cut(s) 296
CviJI RGCY 6 cut(s) 181, 196, 457, 574, 665, 722
CviKI_1 RGCY 6 cut(s) 181, 196, 457, 574, 665, 722
CviQI GTAC 2 cut(s) 63, 708
DdeI CTNAG 3 cut(s) 575, 672, 726
DpnI GATC 3 cut(s) 324, 447, 570
DpnII GATC 3 cut(s) 322, 445, 568
Eam1104I CTCTTC 2 cut(s) 336, 655
EarI CTCTTC 2 cut(s) 336, 655
Eco31I GGTCTC 1 cut(s) 428
Eco57I CTGAAG 1 cut(s) 57
Eco88I CYCGRG 1 cut(s) 564
EcoRII CCWGG 1 cut(s) 411
FaeI CATG 1 cut(s) 299
FatI CATG 1 cut(s) 295
FauNDI CATATG 1 cut(s) 639
FokI GGATG 2 cut(s) 479, 622
FspBI CTAG 1 cut(s) 89
GsaI CCCAGC 1 cut(s) 468
HgaI GACGC 1 cut(s) 230
Hin1II CATG 1 cut(s) 299
HindIII AAGCTT 2 cut(s) 179, 455
HinfI GANTC 3 cut(s) 313, 432, 613
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 1 cut(s) 558
Hpy188I TCNGA 3 cut(s) 76, 450, 497
Hpy188III TCNNGA 3 cut(s) 296, 566, 617
Hpy8I GTNNAC 1 cut(s) 558
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 4 cut(s) 71, 213, 473, 600
HpyCH4III ACNGT 1 cut(s) 332
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 3 cut(s) 209, 305, 404
HpyF10VI GCNNNNNNNGC 1 cut(s) 193
HpyF3I CTNAG 3 cut(s) 575, 672, 726
HpySE526I ACGT 1 cut(s) 56
Hsp92II CATG 1 cut(s) 299
Kzo9I GATC 3 cut(s) 322, 445, 568
LguI GCTCTTC 1 cut(s) 655
MaeI CTAG 1 cut(s) 89
MaeII ACGT 1 cut(s) 56
MaeIII GTNAC 2 cut(s) 52, 690
MalI GATC 3 cut(s) 324, 447, 570
MbiI CCGCTC 1 cut(s) 553
MboI GATC 3 cut(s) 322, 445, 568
MboII GAAGA 8 cut(s) 31, 63, 130, 145, 236, 353, 626, 672
MflI RGATCY 1 cut(s) 445
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 5 cut(s) 306, 362, 398, 517, 599
MmeI TCCRAC 1 cut(s) 207
MnlI CCTC 5 cut(s) 43, 198, 337, 538, 573
MseI TTAA 1 cut(s) 602
MslI CAYNNNNRTG 2 cut(s) 300, 509
MspR9I CCNGG 1 cut(s) 413
MvaI CCWGG 1 cut(s) 413
MwoI GCNNNNNNNGC 1 cut(s) 193
NdeI CATATG 1 cut(s) 639
NdeII GATC 3 cut(s) 322, 445, 568
NlaIII CATG 1 cut(s) 299
NlaIV GGNNCC 1 cut(s) 447
NmeAIII GCCGAG 1 cut(s) 691
NmuCI GTSAC 1 cut(s) 690
NspV TTCGAA 1 cut(s) 311
PagI TCATGA 1 cut(s) 295
PciSI GCTCTTC 1 cut(s) 655
PfeI GAWTC 3 cut(s) 313, 432, 613
PshAI GACNNNNGTC 1 cut(s) 420
Psp6I CCWGG 1 cut(s) 411
PspFI CCCAGC 1 cut(s) 464
PspGI CCWGG 1 cut(s) 411
PspN4I GGNNCC 1 cut(s) 447
PsuI RGATCY 1 cut(s) 445
RsaI GTAC 2 cut(s) 64, 709
RsaNI GTAC 2 cut(s) 63, 708
RseI CAYNNNNRTG 2 cut(s) 300, 509
SapI GCTCTTC 1 cut(s) 655
SaqAI TTAA 1 cut(s) 602
Sau3AI GATC 3 cut(s) 322, 445, 568
ScaI AGTACT 1 cut(s) 709
ScrFI CCNGG 1 cut(s) 413
SduI GDGCHC 1 cut(s) 502
SfuI TTCGAA 1 cut(s) 311
SmiMI CAYNNNNRTG 2 cut(s) 300, 509
Sse9I AATT 5 cut(s) 306, 362, 398, 517, 599
SsiI CCGC 2 cut(s) 157, 553
SspI AATATT 1 cut(s) 275
SspMI CTAG 1 cut(s) 89
StyD4I CCNGG 1 cut(s) 411
TaaI ACNGT 1 cut(s) 332
TaiI ACGT 1 cut(s) 59
TaqI TCGA 4 cut(s) 45, 311, 325, 704
TasI AATT 5 cut(s) 306, 362, 398, 517, 599
TatI WGTACW 1 cut(s) 707
TfiI GAWTC 3 cut(s) 313, 432, 613
Tru1I TTAA 1 cut(s) 602
Tru9I TTAA 1 cut(s) 602
TscAI CASTG 1 cut(s) 335
TseFI GTSAC 1 cut(s) 690
Tsp45I GTSAC 1 cut(s) 690
TspDTI ATGAA 3 cut(s) 31, 626, 626
TspRI CASTG 1 cut(s) 335
XapI RAATTY 1 cut(s) 517
XspI CTAG 1 cut(s) 89
ZrmI AGTACT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.