AT4G10950

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014251
Physical Location & Seq
Reverse (-)
6711386 .. 6713512
2127 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G10950.1

Sequence Viewer

Length: 1203 bp
ATGTCGATCAAGCTTCTCGTTCTTGTTTTCTCTCTTCTGATAATCTTCACGAGACCCAAATTGATCGCCGATCATCACTTAACTACAAGAATCTCGCCGATTTATCCTTCGATTTCTACGTTTCAGCCTTCGATTCCGCCGTTTTTACCTCCATCGCCGTCGCGCAGAGCACAATCTCCGACGGTGAAGCCGTCTTTGCCATTTGTACCGGCGTTGTTCGTTTTCGGAGATTCCTCAGTCGATAGCGGAACCAATAACTTTCTTGGAACCTTAGCGAGAGCAGATCGGCTTCCCTACGGTCGCGATTTCGATACGCATCAACCAACGGGAAGGTTTTGCAATGGAAGAATCCCAGTTGATTATCTAGGCTTACCATTCGTTCCTAGCTATCTTGGACAAACTGGGACTGTTGAAGACATGTTTCAAGGAGTAAACTATGCATCAGCTGGTGCTGGAATCATCTTATCAAGTGGATCTGAATTGGGCCAGCGGGTTTCATTTGCGATGCAAGTTGAGCAGTTTGTTGATACGTTTCAGCAGATGATACTGAGTATTGGGGAGAAAGCTTCGGAACGTTTAGTGTCAAACTCGGTTTTCTACATATCGATTGGAGTGAATGATTACATACATTTCTACATCAGAAACATCTCCAATGTGCAGAATCTCTATACTCCATGGAATTTTAATCAGTTCTTGGCTTCTAATATGAGACAGGAGCTCAAGACCTTGTACAATGTCAAGGTGAGGAGGATGGTGGTGATGGGACTGCCACCTATTGGCTGTGCACCATACTACATGTGGAAATACAGAAGCCAGAACGGAGAATGTGCAGAAGAAGTGAACAGCATGATCATGGAATCCAACTTTGTCATGAGATACACTGTAGATAAACTCAACCGTGAGCTTCCAGGGGCATCCATTATCTACTGTGACGTGTTCCAAAGCGCTATGGACATTCTCAGGAACCACCAACACTACGGTTTTAATGAGACGACAGATGCGTGTTGTGGGCTAGGGAGGTACAAAGGATGGCTTCCATGCATCTCGCCGGAGATGGCTTGCTCTGACGCCTCCGGTCACCTCTGGTGGGACCAGTTTCATCCTACAGACGCCGTTAACGCCATTCTAGCCGACAATGTATGGAATGGTCGCCACGTGGACATGTGTTACCCTACTAACCTAGAAACTATGCTTCATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

400

Amino Acids

45.08

Weight (kDa)

6.36

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 72 - 379 3.7e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10950 AT4G10950
fragaria_vesca FvH4_5g04800
malus_domestica MD06G1109100.v1.1
prunus_persica Prupe.5G122000_v2.0.a1
pyrus_communis pycom06g10240
rosa_chinensis RchiOBHm_Chr7g0194451
rosa_laevigata RLG00000004200
rosa_multiflora Rmu_sc0030308.1_g000001 Rmu_ssc0000261.1_g000027
rosa_roxburghii Rroxscaffold_3G00261350
rosa_rugosa Rorug07G0015700
rosa_samantha Rh7AG143000 Rh7BG143500 Rh7CG147300 Rh7DG144900
rosa_wichuraiana Rw7G012240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 776
AccII CGCG 2 cut(s) 163, 303
AciI CCGC 3 cut(s) 137, 246, 490
AclI AACGTT 1 cut(s) 574
AclWI GGATC 1 cut(s) 481
AcsI RAATTY 1 cut(s) 679
AcvI CACGTG 1 cut(s) 1156
AcyI GRCGYC 2 cut(s) 1068, 1110
AfaI GTAC 3 cut(s) 207, 731, 1022
AfeI AGCGCT 1 cut(s) 946
AfiI CCNNNNNNNGG 2 cut(s) 776, 1087
AflIII ACRYGT 4 cut(s) 417, 795, 933, 1161
AgsI TTSAA 2 cut(s) 413, 425
AjiI CACGTC 1 cut(s) 934
AjnI CCWGG 1 cut(s) 907
AluBI AGCT 6 cut(s) 13, 387, 446, 566, 718, 904
AluI AGCT 6 cut(s) 13, 387, 446, 566, 718, 904
Alw21I GWGCWC 3 cut(s) 172, 720, 787
Alw26I GTCTC 3 cut(s) 46, 703, 983
Alw44I GTGCAC 1 cut(s) 783
AlwI GGATC 1 cut(s) 481
Aor51HI AGCGCT 1 cut(s) 946
AoxI GGCC 1 cut(s) 484
ApaLI GTGCAC 1 cut(s) 783
ApoI RAATTY 1 cut(s) 679
AspLEI GCGC 2 cut(s) 165, 947
AspS9I GGNCC 2 cut(s) 484, 1090
AsuHPI GGTGA 4 cut(s) 196, 754, 769, 1070
AvaII GGWCC 1 cut(s) 1090
BaeGI GKGCMC 1 cut(s) 787
BanII GRGCYC 1 cut(s) 720
BauI CACGAG 1 cut(s) 49
BbrPI CACGTG 1 cut(s) 1156
BbsI GAAGAC 1 cut(s) 420
Bbv12I GWGCWC 3 cut(s) 172, 720, 787
BccI CCATC 5 cut(s) 160, 745, 754, 1023, 1048
BceAI ACGGC 4 cut(s) 124, 142, 175, 1097
BciT130I CCWGG 1 cut(s) 909
BclI TGATCA 1 cut(s) 849
BcoDI GTCTC 3 cut(s) 46, 703, 983
BfaI CTAG 5 cut(s) 365, 384, 1013, 1127, 1181
BfmI CTRYAG 2 cut(s) 882, 1104
BfoI RGCGCY 1 cut(s) 948
Bme1390I CCNGG 1 cut(s) 909
Bme18I GGWCC 1 cut(s) 1090
BmgBI CACGTC 1 cut(s) 934
BmgT120I GGNCC 2 cut(s) 484, 1090
BmiI GGNNCC 4 cut(s) 250, 268, 965, 1091
BmrFI CCNGG 1 cut(s) 909
BmrI ACTGGG 2 cut(s) 347, 411
BmsI GCATC 6 cut(s) 325, 449, 495, 923, 988, 1050
BmuI ACTGGG 2 cut(s) 347, 411
BpiI GAAGAC 1 cut(s) 420
Bpu10I CCTNAGC 1 cut(s) 271
BpuEI CTTGAG 1 cut(s) 704
Bsa29I ATCGAT 1 cut(s) 605
BsaAI YACGTR 1 cut(s) 1156
BsaBI GATNNNNATC 1 cut(s) 315
BsaHI GRCGYC 2 cut(s) 1068, 1110
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 2 cut(s) 674, 908
BsaWI WCCGGW 1 cut(s) 1073
Bsc4I CCNNNNNNNGG 2 cut(s) 776, 1087
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 3 cut(s) 353, 406, 1093
Bse3DI GCAATG 1 cut(s) 346
Bse8I GATNNNNATC 1 cut(s) 315
BseBI CCWGG 1 cut(s) 909
BseCI ATCGAT 1 cut(s) 605
BseDI CCNNGG 2 cut(s) 674, 908
BseGI GGATG 4 cut(s) 756, 914, 1034, 1099
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 2 cut(s) 776, 1087
BseMI GCAATG 1 cut(s) 346
BseMII CTCAG 3 cut(s) 249, 539, 973
BseNI ACTGG 3 cut(s) 353, 406, 1093
BseRI GAGGAG 1 cut(s) 760
BseSI GKGCMC 1 cut(s) 787
BsgI GTGCAG 2 cut(s) 677, 849
Bsh1236I CGCG 2 cut(s) 163, 303
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 1 cut(s) 486
BshVI ATCGAT 1 cut(s) 605
BsiEI CGRYCG 1 cut(s) 301
BsiHKAI GWGCWC 3 cut(s) 172, 720, 787
BsiSI CCGG 3 cut(s) 209, 1049, 1074
BslFI GGGAC 3 cut(s) 418, 777, 1103
BslI CCNNNNNNNGG 2 cut(s) 776, 1087
BsmAI GTCTC 3 cut(s) 46, 703, 983
BsmBI CGTCTC 1 cut(s) 983
BsmFI GGGAC 3 cut(s) 418, 777, 1103
BsnI GGCC 1 cut(s) 486
Bso31I GGTCTC 1 cut(s) 46
Bsp1286I GDGCHC 3 cut(s) 172, 720, 787
Bsp1407I TGTACA 1 cut(s) 729
Bsp143I GATC 6 cut(s) 6, 63, 70, 283, 473, 849
Bsp19I CCATGG 1 cut(s) 674
Bsp68I TCGCGA 1 cut(s) 303
BspACI CCGC 3 cut(s) 137, 246, 490
BspANI GGCC 1 cut(s) 486
BspCNI CTCAG 3 cut(s) 248, 540, 972
BspDI ATCGAT 1 cut(s) 605
BspFNI CGCG 2 cut(s) 163, 303
BspHI TCATGA 1 cut(s) 870
BspLI GGNNCC 4 cut(s) 250, 268, 965, 1091
BspPI GGATC 1 cut(s) 481
BspTNI GGTCTC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 346
BsrFI RCCGGY 1 cut(s) 208
BsrGI TGTACA 1 cut(s) 729
BsrI ACTGG 3 cut(s) 353, 406, 1093
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 2 cut(s) 674, 908
BssMI GATC 6 cut(s) 6, 63, 70, 283, 473, 849
BssNI GRCGYC 2 cut(s) 1068, 1110
BssSI CACGAG 1 cut(s) 49
BssT1I CCWWGG 1 cut(s) 674
Bst2BI CACGAG 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 909
Bst4CI ACNGT 7 cut(s) 184, 299, 409, 883, 899, 929, 980
Bst6I CTCTTC 1 cut(s) 39
BstACI GRCGYC 2 cut(s) 1068, 1110
BstAUI TGTACA 1 cut(s) 729
BstBAI YACGTR 1 cut(s) 1156
BstC8I GCNNGC 2 cut(s) 488, 1060
BstDEI CTNAG 4 cut(s) 235, 271, 548, 959
BstDSI CCRYGG 1 cut(s) 674
BstEII GGTNACC 1 cut(s) 1076
BstF5I GGATG 4 cut(s) 756, 914, 1034, 1099
BstFNI CGCG 2 cut(s) 163, 303
BstH2I RGCGCY 1 cut(s) 948
BstHHI GCGC 2 cut(s) 165, 947
BstKTI GATC 6 cut(s) 9, 66, 73, 286, 476, 852
BstMAI GTCTC 3 cut(s) 46, 703, 983
BstMBI GATC 6 cut(s) 6, 63, 70, 283, 473, 849
BstMCI CGRYCG 1 cut(s) 301
BstMWI GCNNNNNNNGC 4 cut(s) 196, 514, 1118, 1127
BstNI CCWGG 1 cut(s) 909
BstNSI RCATGY 3 cut(s) 421, 799, 1165
BstPI GGTNACC 1 cut(s) 1076
BstSCI CCNGG 1 cut(s) 907
BstSFI CTRYAG 2 cut(s) 882, 1104
BstSLI GKGCMC 1 cut(s) 787
BstUI CGCG 2 cut(s) 163, 303
BstV2I GAAGAC 1 cut(s) 420
BstX2I RGATCY 1 cut(s) 473
BstYI RGATCY 1 cut(s) 473
Bsu15I ATCGAT 1 cut(s) 605
BsuRI GGCC 1 cut(s) 486
BsuTUI ATCGAT 1 cut(s) 605
BtgI CCRYGG 1 cut(s) 674
BtgZI GCGATG 2 cut(s) 138, 518
BtrI CACGTC 1 cut(s) 934
BtsCI GGATG 4 cut(s) 756, 914, 1034, 1099
BtsIMutI CAGTG 1 cut(s) 879
BtuMI TCGCGA 1 cut(s) 303
Cac8I GCNNGC 2 cut(s) 488, 1060
CciI TCATGA 1 cut(s) 870
CfoI GCGC 2 cut(s) 165, 947
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 2 cut(s) 484, 1090
ClaI ATCGAT 1 cut(s) 605
CseI GACGC 2 cut(s) 1076, 1118
Csp6I GTAC 3 cut(s) 206, 730, 1021
CviAII CATG 8 cut(s) 418, 675, 796, 847, 853, 871, 1038, 1162
CviQI GTAC 3 cut(s) 206, 730, 1021
DdeI CTNAG 4 cut(s) 235, 271, 548, 959
DpnI GATC 6 cut(s) 8, 65, 72, 285, 475, 851
DpnII GATC 6 cut(s) 6, 63, 70, 283, 473, 849
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
EciI GGCGGA 1 cut(s) 126
Ecl136II GAGCTC 1 cut(s) 718
Eco130I CCWWGG 1 cut(s) 674
Eco24I GRGCYC 1 cut(s) 720
Eco31I GGTCTC 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 1090
Eco47III AGCGCT 1 cut(s) 946
Eco53kI GAGCTC 1 cut(s) 718
Eco72I CACGTG 1 cut(s) 1156
Eco91I GGTNACC 1 cut(s) 1076
EcoICRI GAGCTC 1 cut(s) 718
EcoO65I GGTNACC 1 cut(s) 1076
EcoRII CCWGG 1 cut(s) 907
EcoT14I CCWWGG 1 cut(s) 674
EcoT22I ATGCAT 2 cut(s) 442, 1043
EcoT38I GRGCYC 1 cut(s) 720
ErhI CCWWGG 1 cut(s) 674
Esp3I CGTCTC 1 cut(s) 983
FaeI CATG 8 cut(s) 421, 678, 799, 850, 856, 874, 1041, 1165
FalI AAGNNNNNCTT 2 cut(s) 1017, 1049
FaqI GGGAC 3 cut(s) 418, 777, 1103
FatI CATG 8 cut(s) 417, 674, 795, 846, 852, 870, 1037, 1161
FauI CCCGC 1 cut(s) 483
FbaI TGATCA 1 cut(s) 849
FokI GGATG 4 cut(s) 763, 901, 1041, 1086
FriOI GRGCYC 1 cut(s) 720
FspBI CTAG 5 cut(s) 365, 384, 1013, 1127, 1181
GlaI GCGC 2 cut(s) 164, 946
HaeII RGCGCY 1 cut(s) 948
HaeIII GGCC 1 cut(s) 486
HapII CCGG 3 cut(s) 209, 1049, 1074
HgaI GACGC 2 cut(s) 1076, 1118
HhaI GCGC 2 cut(s) 165, 947
Hin1I GRCGYC 2 cut(s) 1068, 1110
Hin1II CATG 8 cut(s) 421, 678, 799, 850, 856, 874, 1041, 1165
Hin6I GCGC 2 cut(s) 163, 945
HinP1I GCGC 2 cut(s) 163, 945
HincII GTYRAC 1 cut(s) 1117
HindII GTYRAC 1 cut(s) 1117
HindIII AAGCTT 2 cut(s) 11, 564
HinfI GANTC 7 cut(s) 90, 133, 230, 348, 456, 661, 857
HpaI GTTAAC 1 cut(s) 1117
HpaII CCGG 3 cut(s) 209, 1049, 1074
HphI GGTGA 4 cut(s) 196, 754, 769, 1070
Hpy166II GTNNAC 5 cut(s) 433, 785, 841, 1117, 1159
Hpy188I TCNGA 7 cut(s) 39, 180, 227, 478, 571, 641, 1066
Hpy188III TCNNGA 5 cut(s) 49, 302, 721, 871, 961
Hpy8I GTNNAC 5 cut(s) 433, 785, 841, 1117, 1159
Hpy99I CGWCG 2 cut(s) 163, 184
HpyAV CCTTC 3 cut(s) 117, 138, 324
HpyCH4III ACNGT 7 cut(s) 184, 299, 409, 883, 899, 929, 980
HpyCH4IV ACGT 5 cut(s) 119, 530, 574, 933, 1155
HpyCH4V TGCA 7 cut(s) 339, 440, 508, 658, 785, 830, 1041
HpyF10VI GCNNNNNNNGC 4 cut(s) 196, 514, 1118, 1127
HpyF3I CTNAG 4 cut(s) 235, 271, 548, 959
HpySE526I ACGT 5 cut(s) 119, 530, 574, 933, 1155
Hsp92I GRCGYC 2 cut(s) 1068, 1110
Hsp92II CATG 8 cut(s) 421, 678, 799, 850, 856, 874, 1041, 1165
HspAI GCGC 2 cut(s) 163, 945
Ksp22I TGATCA 1 cut(s) 849
KspAI GTTAAC 1 cut(s) 1117
Kzo9I GATC 6 cut(s) 6, 63, 70, 283, 473, 849
LmnI GCTCC 1 cut(s) 715
LweI GCATC 6 cut(s) 325, 449, 495, 923, 988, 1050
MaeI CTAG 5 cut(s) 365, 384, 1013, 1127, 1181
MaeII ACGT 5 cut(s) 119, 530, 574, 933, 1155
MaeIII GTNAC 3 cut(s) 929, 1076, 1166
MalI GATC 6 cut(s) 8, 65, 72, 285, 475, 851
MboI GATC 6 cut(s) 6, 63, 70, 283, 473, 849
MboII GAAGA 5 cut(s) 26, 37, 357, 425, 845
MflI RGATCY 1 cut(s) 473
MhlI GDGCHC 3 cut(s) 172, 720, 787
MluCI AATT 3 cut(s) 59, 479, 679
MmeI TCCRAC 2 cut(s) 203, 885
MnlI CCTC 7 cut(s) 159, 244, 738, 741, 1011, 1081, 1091
Mph1103I ATGCAT 2 cut(s) 442, 1043
MseI TTAA 5 cut(s) 80, 684, 984, 1116, 1201
MslI CAYNNNNRTG 1 cut(s) 851
MspA1I CMGCKG 2 cut(s) 446, 490
MspI CCGG 3 cut(s) 209, 1049, 1074
MspR9I CCNGG 1 cut(s) 909
MvaI CCWGG 1 cut(s) 909
MvnI CGCG 2 cut(s) 163, 303
MwoI GCNNNNNNNGC 4 cut(s) 196, 514, 1118, 1127
NcoI CCATGG 1 cut(s) 674
NdeII GATC 6 cut(s) 6, 63, 70, 283, 473, 849
NlaIII CATG 8 cut(s) 421, 678, 799, 850, 856, 874, 1041, 1165
NlaIV GGNNCC 4 cut(s) 250, 268, 965, 1091
NmuCI GTSAC 2 cut(s) 929, 1076
NruI TCGCGA 1 cut(s) 303
NsiI ATGCAT 2 cut(s) 442, 1043
NspI RCATGY 3 cut(s) 421, 799, 1165
PagI TCATGA 1 cut(s) 870
PciI ACATGT 3 cut(s) 417, 795, 1161
PcsI WCGNNNNNNNCGW 4 cut(s) 116, 137, 188, 998
PfeI GAWTC 7 cut(s) 90, 133, 230, 348, 456, 661, 857
PflMI CCANNNNNTGG 1 cut(s) 776
PmaCI CACGTG 1 cut(s) 1156
PmlI CACGTG 1 cut(s) 1156
Ppu21I YACGTR 1 cut(s) 1156
PscI ACATGT 3 cut(s) 417, 795, 1161
Psp124BI GAGCTC 1 cut(s) 720
Psp1406I AACGTT 1 cut(s) 574
Psp6I CCWGG 1 cut(s) 907
PspCI CACGTG 1 cut(s) 1156
PspEI GGTNACC 1 cut(s) 1076
PspGI CCWGG 1 cut(s) 907
PspN4I GGNNCC 4 cut(s) 250, 268, 965, 1091
PspPI GGNCC 2 cut(s) 484, 1090
PsuI RGATCY 1 cut(s) 473
PvuII CAGCTG 1 cut(s) 446
RruI TCGCGA 1 cut(s) 303
RsaI GTAC 3 cut(s) 207, 731, 1022
RsaNI GTAC 3 cut(s) 206, 730, 1021
RseI CAYNNNNRTG 1 cut(s) 851
SacI GAGCTC 1 cut(s) 720
SaqAI TTAA 5 cut(s) 80, 684, 984, 1116, 1201
Sau3AI GATC 6 cut(s) 6, 63, 70, 283, 473, 849
Sau96I GGNCC 2 cut(s) 484, 1090
ScrFI CCNGG 1 cut(s) 909
SduI GDGCHC 3 cut(s) 172, 720, 787
SfaNI GCATC 6 cut(s) 325, 449, 495, 923, 988, 1050
SfcI CTRYAG 2 cut(s) 882, 1104
SinI GGWCC 1 cut(s) 1090
SmiMI CAYNNNNRTG 1 cut(s) 851
SmlI CTYRAG 1 cut(s) 719
SmoI CTYRAG 1 cut(s) 719
Sse9I AATT 3 cut(s) 59, 479, 679
SsiI CCGC 3 cut(s) 137, 246, 490
SspMI CTAG 5 cut(s) 365, 384, 1013, 1127, 1181
SstI GAGCTC 1 cut(s) 720
StyD4I CCNGG 1 cut(s) 907
StyI CCWWGG 1 cut(s) 674
TaaI ACNGT 7 cut(s) 184, 299, 409, 883, 899, 929, 980
TaiI ACGT 5 cut(s) 122, 533, 577, 936, 1158
TaqI TCGA 6 cut(s) 5, 110, 131, 240, 309, 605
TasI AATT 3 cut(s) 59, 479, 679
TatI WGTACW 1 cut(s) 729
TfiI GAWTC 7 cut(s) 90, 133, 230, 348, 456, 661, 857
Tru1I TTAA 5 cut(s) 80, 684, 984, 1116, 1201
Tru9I TTAA 5 cut(s) 80, 684, 984, 1116, 1201
TscAI CASTG 1 cut(s) 886
TseFI GTSAC 2 cut(s) 929, 1076
Tsp45I GTSAC 2 cut(s) 929, 1076
TspDTI ATGAA 3 cut(s) 486, 1088, 1184
TspGWI ACGGA 1 cut(s) 834
TspRI CASTG 1 cut(s) 886
Van91I CCANNNNNTGG 1 cut(s) 776
VneI GTGCAC 1 cut(s) 783
VpaK11BI GGWCC 1 cut(s) 1090
XapI RAATTY 1 cut(s) 679
XceI RCATGY 3 cut(s) 421, 799, 1165
XcmI CCANNNNNNNNNTGG 1 cut(s) 795
XspI CTAG 5 cut(s) 365, 384, 1013, 1127, 1181
Zsp2I ATGCAT 2 cut(s) 442, 1043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.