Rmu_sc0030308.1_g000001

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0030308.1
OG0014251
Physical Location & Seq
Reverse (-)
1131 .. 4410
3280 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0030308.1_g000001.1.cds

Sequence Viewer

Length: 1209 bp
atgaggttttttctaatttctttgtggaattttggatcagcattgcggcttgggcttccttttgtacccagttttcttgggcagactggaagagtggaagatatgcttcatggattgaactttgcttctgctgctgctggaattatattcaccagtgggtcagaattgggtcaacatgtttccttgtctcagcaaattcagcagtttacagatacttacgagcagtttgtgttgagtatggggcagcaagctgcgattaatctcatctcgaattcggtgctctacatttcaatcggaatcaatgactacatacattactacttgcgaaatgagtcgaacgtgcaaaatctctatttgccctggagtttcaatcgatttctagcatccacggtgacgcaggaaatcaaggtgagaattaatgtcaggaaggtggttgtgatgggtctgccacctataggttgtgctcctcactacttatgtgagtacaaagtgaaggatggacaatgtgttgaggagataaacaacatggtattggagttcaactttatcatgaggtatatgattgaagagctaagtgaagcgcttccgtattcccatatcatcttctgtgatgtgttccaaggatccatggatatgttaaaggactctgaacgttacggtttcaaagtcaccaccgatgcttgctgcggattaggcgactacaagggttttctcatgtgcttatcaccagacatggcttgcagtaatgcatctgaccatatctggtgggaccaattccatccaactgatgcagttaatgccattctggctgataatgtctggaatggcctgcacacgaaaatgtgctatccaatgaacttggaggatgtggcaccaaggctccaatggagaagtgtaaagtctggaactgtgacttcttcagcttcctgcctccttcgaactcactcctccgccgccttctccacacctagaaaaacattgtctccgccggagatcacgacgctagggacgagcctctttcgcctcgattccactgcagcccaccgtagaacagtcatcttccgcgagattcgggtccgatttcaaggtatttcgaagcggggagagagggttcttgttcggcccagtctctccatgtccggccagctcgaccgtgggctagtggcgtggctcagctctgagaatctgtttgcagtcgaccagtcgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

402

Amino Acids

45.28

Weight (kDa)

6.55

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10950 AT4G10950
fragaria_vesca FvH4_5g04800
malus_domestica MD06G1109100.v1.1
prunus_persica Prupe.5G122000_v2.0.a1
pyrus_communis pycom06g10240
rosa_chinensis RchiOBHm_Chr7g0194451
rosa_laevigata RLG00000004200
rosa_multiflora Rmu_sc0030308.1_g000001 Rmu_ssc0000261.1_g000027
rosa_roxburghii Rroxscaffold_3G00261350
rosa_rugosa Rorug07G0015700
rosa_samantha Rh7AG143000 Rh7BG143500 Rh7CG147300 Rh7DG144900
rosa_wichuraiana Rw7G012240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 871
AccI GTMKAC 1 cut(s) 1197
AccII CGCG 1 cut(s) 1065
AciI CCGC 7 cut(s) 46, 687, 951, 954, 986, 1063, 1099
AclI AACGTT 1 cut(s) 652
AclWI GGATC 3 cut(s) 43, 618, 631
AcoI YGGCCR 1 cut(s) 1141
AcsI RAATTY 3 cut(s) 28, 195, 271
AcuI CTGAAG 1 cut(s) 903
AfaI GTAC 2 cut(s) 66, 485
AfeI AGCGCT 1 cut(s) 582
AfiI CCNNNNNNNGG 1 cut(s) 455
AflIII ACRYGT 1 cut(s) 175
AgsI TTSAA 7 cut(s) 118, 291, 370, 541, 566, 664, 1085
AjnI CCWGG 1 cut(s) 359
AloI GAACNNNNNNTCC 2 cut(s) 1095, 1127
AluBI AGCT 5 cut(s) 251, 571, 923, 1147, 1176
AluI AGCT 5 cut(s) 251, 571, 923, 1147, 1176
Alw21I GWGCWC 2 cut(s) 282, 466
Alw26I GTCTC 3 cut(s) 192, 987, 1133
AlwI GGATC 3 cut(s) 43, 618, 631
Aor51HI AGCGCT 1 cut(s) 582
AoxI GGCC 3 cut(s) 826, 1121, 1141
ApeKI GCWGC 6 cut(s) 131, 134, 244, 251, 684, 1037
ApoI RAATTY 3 cut(s) 28, 195, 271
AseI ATTAAT 2 cut(s) 258, 417
Asp700I GAANNNNTTC 1 cut(s) 582
AspLEI GCGC 1 cut(s) 583
AspS9I GGNCC 3 cut(s) 769, 1075, 1122
AsuHPI GGTGA 5 cut(s) 142, 403, 421, 661, 717
AsuII TTCGAA 2 cut(s) 937, 1094
AvaII GGWCC 2 cut(s) 769, 1075
BamHI GGATCC 1 cut(s) 623
BanI GGYRCC 1 cut(s) 871
Bbv12I GWGCWC 2 cut(s) 282, 466
BbvI GCAGC 6 cut(s) 118, 121, 238, 256, 671, 1049
BccI CCATC 3 cut(s) 433, 491, 786
BcgI CGANNNNNNTGC 4 cut(s) 259, 293, 1016, 1050
BciT130I CCWGG 1 cut(s) 361
BcoDI GTCTC 3 cut(s) 192, 987, 1133
BfaI CTAG 4 cut(s) 380, 969, 1004, 1160
BfmI CTRYAG 2 cut(s) 453, 1035
BfoI RGCGCY 1 cut(s) 584
BglI GCCNNNNNGGC 1 cut(s) 806
BisI GCNGC 8 cut(s) 47, 132, 135, 245, 252, 685, 954, 1038
BlpI GCTNAGC 1 cut(s) 1172
BlsI GCNGC 8 cut(s) 48, 133, 136, 246, 253, 686, 955, 1039
Bme1390I CCNGG 1 cut(s) 361
Bme18I GGWCC 2 cut(s) 769, 1075
BmgT120I GGNCC 3 cut(s) 769, 1075, 1122
BmiI GGNNCC 5 cut(s) 625, 770, 873, 881, 1076
BmrFI CCNGG 1 cut(s) 361
BmrI ACTGGG 2 cut(s) 63, 1119
BmsI GCATC 4 cut(s) 392, 667, 758, 778
BmuI ACTGGG 2 cut(s) 63, 1119
BpmI CTGGAG 1 cut(s) 382
Bpu1102I GCTNAGC 1 cut(s) 1172
Bpu14I TTCGAA 2 cut(s) 937, 1094
Bsa29I ATCGAT 1 cut(s) 373
BsaJI CCNNGG 6 cut(s) 359, 387, 619, 627, 875, 1153
BsaXI ACNNNNNCTCC 6 cut(s) 864, 894, 967, 997, 1095, 1125
Bsc4I CCNNNNNNNGG 1 cut(s) 455
Bse1I ACTGG 5 cut(s) 69, 91, 153, 1125, 1201
Bse3DI GCAATG 1 cut(s) 41
BseBI CCWGG 1 cut(s) 361
BseCI ATCGAT 1 cut(s) 373
BseDI CCNNGG 6 cut(s) 359, 387, 619, 627, 875, 1153
BseGI GGATG 4 cut(s) 383, 502, 778, 871
BseLI CCNNNNNNNGG 1 cut(s) 455
BseMI GCAATG 1 cut(s) 41
BseMII CTCAG 3 cut(s) 203, 1170, 1186
BseNI ACTGG 5 cut(s) 69, 91, 153, 1125, 1201
BseRI GAGGAG 3 cut(s) 456, 527, 937
BseXI GCAGC 6 cut(s) 118, 121, 238, 256, 671, 1049
BsgI GTGCAG 1 cut(s) 815
Bsh1236I CGCG 1 cut(s) 1065
Bsh1285I CGRYCG 1 cut(s) 1153
BshFI GGCC 3 cut(s) 828, 1123, 1143
BshNI GGYRCC 1 cut(s) 871
BshVI ATCGAT 1 cut(s) 373
BsiEI CGRYCG 1 cut(s) 1153
BsiHKAI GWGCWC 2 cut(s) 282, 466
BsiSI CCGG 2 cut(s) 989, 1140
BslFI GGGAC 2 cut(s) 782, 1021
BslI CCNNNNNNNGG 1 cut(s) 455
BsmAI GTCTC 3 cut(s) 192, 987, 1133
BsmFI GGGAC 2 cut(s) 782, 1021
BsnI GGCC 3 cut(s) 828, 1123, 1143
Bsp119I TTCGAA 2 cut(s) 937, 1094
Bsp1286I GDGCHC 2 cut(s) 282, 466
Bsp143I GATC 3 cut(s) 35, 623, 993
Bsp1720I GCTNAGC 1 cut(s) 1172
Bsp19I CCATGG 1 cut(s) 627
BspACI CCGC 7 cut(s) 46, 687, 951, 954, 986, 1063, 1099
BspANI GGCC 3 cut(s) 828, 1123, 1143
BspCNI CTCAG 3 cut(s) 202, 1171, 1185
BspDI ATCGAT 1 cut(s) 373
BspFNI CGCG 1 cut(s) 1065
BspHI TCATGA 1 cut(s) 549
BspLI GGNNCC 5 cut(s) 625, 770, 873, 881, 1076
BspMAI CTGCAG 1 cut(s) 1039
BspPI GGATC 3 cut(s) 43, 618, 631
BspQI GCTCTTC 1 cut(s) 561
BspT104I TTCGAA 2 cut(s) 937, 1094
BspT107I GGYRCC 1 cut(s) 871
BsrDI GCAATG 1 cut(s) 41
BsrI ACTGG 5 cut(s) 69, 91, 153, 1125, 1201
BssECI CCNNGG 6 cut(s) 359, 387, 619, 627, 875, 1153
BssMI GATC 3 cut(s) 35, 623, 993
BssT1I CCWWGG 3 cut(s) 619, 627, 875
Bst2UI CCWGG 1 cut(s) 361
Bst4CI ACNGT 6 cut(s) 391, 659, 910, 1046, 1054, 1154
Bst6I CTCTTC 2 cut(s) 85, 561
BstAPI GCANNNNNTGC 1 cut(s) 797
BstBI TTCGAA 2 cut(s) 937, 1094
BstC8I GCNNGC 5 cut(s) 249, 682, 739, 830, 1145
BstDEI CTNAG 4 cut(s) 189, 572, 1172, 1179
BstDSI CCRYGG 3 cut(s) 387, 627, 1153
BstF5I GGATG 4 cut(s) 383, 502, 778, 871
BstFNI CGCG 1 cut(s) 1065
BstH2I RGCGCY 1 cut(s) 584
BstHHI GCGC 1 cut(s) 583
BstKTI GATC 3 cut(s) 38, 626, 996
BstMAI GTCTC 3 cut(s) 192, 987, 1133
BstMBI GATC 3 cut(s) 35, 623, 993
BstMCI CGRYCG 1 cut(s) 1153
BstMWI GCNNNNNNNGC 7 cut(s) 52, 131, 199, 693, 797, 806, 1020
BstNI CCWGG 1 cut(s) 361
BstNSI RCATGY 1 cut(s) 179
BstSCI CCNGG 1 cut(s) 359
BstSFI CTRYAG 2 cut(s) 453, 1035
BstUI CGCG 1 cut(s) 1065
BstV1I GCAGC 6 cut(s) 118, 121, 238, 256, 671, 1049
BstX2I RGATCY 1 cut(s) 623
BstYI RGATCY 1 cut(s) 623
Bsu15I ATCGAT 1 cut(s) 373
BsuRI GGCC 3 cut(s) 828, 1123, 1143
BsuTUI ATCGAT 1 cut(s) 373
BtgI CCRYGG 3 cut(s) 387, 627, 1153
BtsCI GGATG 4 cut(s) 383, 502, 778, 871
BtsI GCAGTG 1 cut(s) 1032
BtsIMutI CAGTG 2 cut(s) 160, 1032
Cac8I GCNNGC 5 cut(s) 249, 682, 739, 830, 1145
CciI TCATGA 1 cut(s) 549
CfoI GCGC 1 cut(s) 583
Cfr13I GGNCC 3 cut(s) 769, 1075, 1122
ClaI ATCGAT 1 cut(s) 373
CseI GACGC 2 cut(s) 403, 1009
Csp6I GTAC 2 cut(s) 65, 484
CviAII CATG 8 cut(s) 110, 176, 526, 550, 628, 715, 733, 1135
CviQI GTAC 2 cut(s) 65, 484
DdeI CTNAG 4 cut(s) 189, 572, 1172, 1179
DpnI GATC 3 cut(s) 37, 625, 995
DpnII GATC 3 cut(s) 35, 623, 993
EaeI YGGCCR 1 cut(s) 1141
Eam1104I CTCTTC 2 cut(s) 85, 561
EarI CTCTTC 2 cut(s) 85, 561
EciI GGCGGA 2 cut(s) 940, 975
Eco130I CCWWGG 3 cut(s) 619, 627, 875
Eco47I GGWCC 2 cut(s) 769, 1075
Eco47III AGCGCT 1 cut(s) 582
Eco57I CTGAAG 1 cut(s) 903
EcoRI GAATTC 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 359
EcoT14I CCWWGG 3 cut(s) 619, 627, 875
EcoT22I ATGCAT 1 cut(s) 751
ErhI CCWWGG 3 cut(s) 619, 627, 875
FaeI CATG 8 cut(s) 113, 179, 529, 553, 631, 718, 736, 1138
FalI AAGNNNNNCTT 2 cut(s) 90, 122
FaqI GGGAC 2 cut(s) 782, 1021
FatI CATG 8 cut(s) 109, 175, 525, 549, 627, 714, 732, 1134
FauI CCCGC 1 cut(s) 1092
FblI GTMKAC 1 cut(s) 1197
Fnu4HI GCNGC 8 cut(s) 47, 132, 135, 245, 252, 685, 954, 1038
FokI GGATG 4 cut(s) 370, 509, 765, 878
Fsp4HI GCNGC 8 cut(s) 47, 132, 135, 245, 252, 685, 954, 1038
FspBI CTAG 4 cut(s) 380, 969, 1004, 1160
GlaI GCGC 1 cut(s) 582
GluI GCNGC 8 cut(s) 47, 132, 135, 245, 252, 685, 954, 1038
GsuI CTGGAG 1 cut(s) 382
HaeII RGCGCY 1 cut(s) 584
HaeIII GGCC 3 cut(s) 828, 1123, 1143
HapII CCGG 2 cut(s) 989, 1140
HgaI GACGC 2 cut(s) 403, 1009
HhaI GCGC 1 cut(s) 583
Hin1II CATG 8 cut(s) 113, 179, 529, 553, 631, 718, 736, 1138
Hin6I GCGC 1 cut(s) 581
HinP1I GCGC 1 cut(s) 581
HincII GTYRAC 2 cut(s) 173, 1198
HindII GTYRAC 2 cut(s) 173, 1198
HinfI GANTC 6 cut(s) 297, 332, 644, 1028, 1069, 1183
HpaII CCGG 2 cut(s) 989, 1140
HphI GGTGA 5 cut(s) 142, 403, 421, 661, 717
Hpy166II GTNNAC 3 cut(s) 173, 207, 1198
Hpy188I TCNGA 6 cut(s) 163, 296, 649, 754, 1079, 1180
Hpy188III TCNNGA 7 cut(s) 268, 424, 550, 820, 903, 997, 1206
Hpy8I GTNNAC 3 cut(s) 173, 207, 1198
Hpy99I CGWCG 1 cut(s) 1003
HpyAV CCTTC 4 cut(s) 421, 487, 944, 967
HpyCH4III ACNGT 6 cut(s) 391, 659, 910, 1046, 1054, 1154
HpyCH4IV ACGT 2 cut(s) 339, 652
HpyCH4V TGCA 7 cut(s) 343, 741, 749, 791, 832, 1037, 1193
HpyF10VI GCNNNNNNNGC 7 cut(s) 52, 131, 199, 693, 797, 806, 1020
HpyF3I CTNAG 4 cut(s) 189, 572, 1172, 1179
HpySE526I ACGT 2 cut(s) 339, 652
Hsp92II CATG 8 cut(s) 113, 179, 529, 553, 631, 718, 736, 1138
HspAI GCGC 1 cut(s) 581
Kzo9I GATC 3 cut(s) 35, 623, 993
LguI GCTCTTC 1 cut(s) 561
LmnI GCTCC 2 cut(s) 469, 885
Lsp1109I GCAGC 6 cut(s) 118, 121, 238, 256, 671, 1049
LweI GCATC 4 cut(s) 392, 667, 758, 778
MaeI CTAG 4 cut(s) 380, 969, 1004, 1160
MaeII ACGT 2 cut(s) 339, 652
MaeIII GTNAC 4 cut(s) 391, 653, 667, 910
MalI GATC 3 cut(s) 37, 625, 995
MboI GATC 3 cut(s) 35, 623, 993
MboII GAAGA 6 cut(s) 102, 110, 578, 595, 909, 1051
MflI RGATCY 1 cut(s) 623
MhlI GDGCHC 2 cut(s) 282, 466
MluCI AATT 8 cut(s) 15, 28, 141, 164, 195, 271, 414, 773
MlyI GAGTC 2 cut(s) 341, 638
MmeI TCCRAC 1 cut(s) 806
MnlI CCTC 9 cut(s) 477, 505, 546, 856, 941, 958, 1025, 1034, 1101
Mph1103I ATGCAT 1 cut(s) 751
MroXI GAANNNNTTC 1 cut(s) 582
MseI TTAA 4 cut(s) 258, 417, 638, 795
MslI CAYNNNNRTG 2 cut(s) 632, 839
MspI CCGG 2 cut(s) 989, 1140
MspR9I CCNGG 1 cut(s) 361
MvaI CCWGG 1 cut(s) 361
MvnI CGCG 1 cut(s) 1065
MwoI GCNNNNNNNGC 7 cut(s) 52, 131, 199, 693, 797, 806, 1020
NcoI CCATGG 1 cut(s) 627
NdeII GATC 3 cut(s) 35, 623, 993
NlaIII CATG 8 cut(s) 113, 179, 529, 553, 631, 718, 736, 1138
NlaIV GGNNCC 5 cut(s) 625, 770, 873, 881, 1076
NmuCI GTSAC 3 cut(s) 391, 667, 910
NsiI ATGCAT 1 cut(s) 751
NspI RCATGY 1 cut(s) 179
NspV TTCGAA 2 cut(s) 937, 1094
PagI TCATGA 1 cut(s) 549
PciI ACATGT 1 cut(s) 175
PciSI GCTCTTC 1 cut(s) 561
PcsI WCGNNNNNNNCGW 1 cut(s) 1007
PdmI GAANNNNTTC 1 cut(s) 582
PfeI GAWTC 4 cut(s) 297, 1028, 1069, 1183
PkrI GCNGC 8 cut(s) 48, 133, 136, 246, 253, 686, 955, 1039
PleI GAGTC 2 cut(s) 340, 638
PpsI GAGTC 2 cut(s) 340, 638
PscI ACATGT 1 cut(s) 175
PshBI ATTAAT 2 cut(s) 258, 417
Psp1406I AACGTT 1 cut(s) 652
Psp6I CCWGG 1 cut(s) 359
PspGI CCWGG 1 cut(s) 359
PspN4I GGNNCC 5 cut(s) 625, 770, 873, 881, 1076
PspPI GGNCC 3 cut(s) 769, 1075, 1122
PstI CTGCAG 1 cut(s) 1039
PsuI RGATCY 1 cut(s) 623
RsaI GTAC 2 cut(s) 66, 485
RsaNI GTAC 2 cut(s) 65, 484
RseI CAYNNNNRTG 2 cut(s) 632, 839
SalI GTCGAC 1 cut(s) 1196
SapI GCTCTTC 1 cut(s) 561
SaqAI TTAA 4 cut(s) 258, 417, 638, 795
SatI GCNGC 8 cut(s) 47, 132, 135, 245, 252, 685, 954, 1038
Sau3AI GATC 3 cut(s) 35, 623, 993
Sau96I GGNCC 3 cut(s) 769, 1075, 1122
SchI GAGTC 2 cut(s) 341, 638
ScrFI CCNGG 1 cut(s) 361
SduI GDGCHC 2 cut(s) 282, 466
SfaNI GCATC 4 cut(s) 392, 667, 758, 778
SfcI CTRYAG 2 cut(s) 453, 1035
SfuI TTCGAA 2 cut(s) 937, 1094
SinI GGWCC 2 cut(s) 769, 1075
SmiMI CAYNNNNRTG 2 cut(s) 632, 839
Sse9I AATT 8 cut(s) 15, 28, 141, 164, 195, 271, 414, 773
SsiI CCGC 7 cut(s) 46, 687, 951, 954, 986, 1063, 1099
SspMI CTAG 4 cut(s) 380, 969, 1004, 1160
StyD4I CCNGG 1 cut(s) 359
StyI CCWWGG 3 cut(s) 619, 627, 875
TaaI ACNGT 6 cut(s) 391, 659, 910, 1046, 1054, 1154
TaiI ACGT 2 cut(s) 342, 655
TaqI TCGA 8 cut(s) 269, 335, 373, 937, 1026, 1094, 1149, 1197
TasI AATT 8 cut(s) 15, 28, 141, 164, 195, 271, 414, 773
TatI WGTACW 1 cut(s) 483
TauI GCSGC 2 cut(s) 49, 956
TfiI GAWTC 4 cut(s) 297, 1028, 1069, 1183
Tru1I TTAA 4 cut(s) 258, 417, 638, 795
Tru9I TTAA 4 cut(s) 258, 417, 638, 795
TscAI CASTG 2 cut(s) 160, 1039
TseFI GTSAC 3 cut(s) 391, 667, 910
TseI GCWGC 6 cut(s) 131, 134, 244, 251, 684, 1037
Tsp45I GTSAC 3 cut(s) 391, 667, 910
TspDTI ATGAA 2 cut(s) 98, 869
TspGWI ACGGA 1 cut(s) 576
TspRI CASTG 2 cut(s) 160, 1039
VpaK11BI GGWCC 2 cut(s) 769, 1075
VspI ATTAAT 2 cut(s) 258, 417
XapI RAATTY 3 cut(s) 28, 195, 271
XceI RCATGY 1 cut(s) 179
XcmI CCANNNNNNNNNTGG 2 cut(s) 882, 1151
XmiI GTMKAC 1 cut(s) 1197
XmnI GAANNNNTTC 1 cut(s) 582
XspI CTAG 4 cut(s) 380, 969, 1004, 1160
Zsp2I ATGCAT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.