FvH4_5g04800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014251
Physical Location & Seq
Forward (+)
2861697 .. 2864141
2445 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04800.t1

Sequence Viewer

Length: 1185 bp
ATGGCGATGATCAATCTTCTTCTCTCTTTCCTCTTCTTTCTCACCACCGCCGTCGTTTCACAACCCACCACTGCTCCGCCGTCTCAAGAACCCATCGCCGCCGCCCCCATCTCTCCACCCTCTCCTCTTCTTCCAATCTCCCAAACCCCACCGCCCAAGACTCCTCTCGTCCCTGCCCTCTTCGTCATCGGCGACTCCTCCGTCGACTCCGGCACCAACAACTTCCTAGGGACATTCGCACGCGCCGACCGCCTCCCCTACGGCCGTGACTTCGACACCCACCAGCCCACTGGGCGGTTCTGCAACGGCAGAATCCCAGTCGATTATCTTGCATTGCGGCTTGGGCTTCCTTTTGTACCCAGTTTTCTTGGGCAGACGGGAAGAGTGGAAGATATGCTTCATGGATTGAACTTTGCTTCTGCTGCTGCTGGAATTATATTCACTAGTGGGTCTGAATTGGGGCAACATGTTTCGTTGTCGCAACAGATTCAGCAGTTTACAGATACATATCAGCAGTTTGTGTTGAGTATGGGGCAGCAAGCTGCGGTTGATCTCATCTCGAATTCGGTGCTCTACATTTCGATTGGAATAAATGACTACATACATTACTACCTGCGTAATGAGTCCAACGTGCAAAATGTATATTTTCCCTGGAGTTTCAATCGGTTTCTAGCATCCACTGTGAAGCAGGAAATCAAGAACTTGTACAGCATTAATGTCAGGAAGGTGGTTGTGATGGGTCTGCCACCTATAGGCTGTGCTCCTCACTACCTATGTGAGTACGAAGTGAAGGATGGACAATGTGTTGAGGAGATAAACAACATGGTAATGGAGTTCAACTTTGTCATGAGGTATATGATTGGAGAGCTAAATGAAGAGCTTCCATATTCCCATATCATCTTCTGTGATGTGTTCCAAGGATCCATGGATATGTTAAAGAACTCTGAACAATACGGTTTTAAAGTCACCACCGATGCCTGCTGCGGATTGGGTGACTACAAGGGTTTTCTCATGTGCTTATCACCAGACATGGCTTGCAGTAATGCTTCTGACCACATCTGGTGGGACCAATTCCATCCAACTGATACAGTTAACGCCATTCTGGCTGATAATGTCTGGAATGGCCTGCACACGAAAATGTGCTATCCGATGAACTTGGAGGATGTGGTGGCATCGACATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

395

Amino Acids

43.87

Weight (kDa)

4.82

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 60 - 371 1.4e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10950 AT4G10950
fragaria_vesca FvH4_5g04800
malus_domestica MD06G1109100.v1.1
prunus_persica Prupe.5G122000_v2.0.a1
pyrus_communis pycom06g10240
rosa_chinensis RchiOBHm_Chr7g0194451
rosa_laevigata RLG00000004200
rosa_multiflora Rmu_sc0030308.1_g000001 Rmu_ssc0000261.1_g000027
rosa_roxburghii Rroxscaffold_3G00261350
rosa_rugosa Rorug07G0015700
rosa_samantha Rh7AG143000 Rh7BG143500 Rh7CG147300 Rh7DG144900
rosa_wichuraiana Rw7G012240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 200
Acc36I ACCTGC 1 cut(s) 621
AccB1I GGYRCC 1 cut(s) 212
AccI GTMKAC 1 cut(s) 204
AccII CGCG 1 cut(s) 243
AclWI GGATC 2 cut(s) 915, 928
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 1 cut(s) 562
AfaI GTAC 3 cut(s) 357, 707, 782
AfiI CCNNNNNNNGG 2 cut(s) 294, 752
AflIII ACRYGT 1 cut(s) 466
AgsI TTSAA 3 cut(s) 409, 661, 838
AhlI ACTAGT 1 cut(s) 443
AjnI CCWGG 1 cut(s) 650
AluBI AGCT 3 cut(s) 542, 868, 880
AluI AGCT 3 cut(s) 542, 868, 880
Alw21I GWGCWC 2 cut(s) 573, 763
Alw26I GTCTC 1 cut(s) 87
AlwI GGATC 2 cut(s) 915, 928
AoxI GGCC 2 cut(s) 262, 1123
ApeKI GCWGC 5 cut(s) 422, 425, 535, 542, 981
ApoI RAATTY 1 cut(s) 562
AseI ATTAAT 1 cut(s) 714
Asp700I GAANNNNTTC 1 cut(s) 879
AspA2I CCTAGG 1 cut(s) 226
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 1 cut(s) 1066
AsuHPI GGTGA 4 cut(s) 34, 958, 1004, 1014
AvaII GGWCC 1 cut(s) 1066
AvrII CCTAGG 1 cut(s) 226
BamHI GGATCC 1 cut(s) 920
BanI GGYRCC 1 cut(s) 212
Bbv12I GWGCWC 2 cut(s) 573, 763
BbvI GCAGC 5 cut(s) 409, 412, 529, 547, 968
BccI CCATC 5 cut(s) 101, 116, 730, 788, 1083
BceAI ACGGC 5 cut(s) 35, 64, 249, 277, 322
BcgI CGANNNNNNTGC 4 cut(s) 311, 345, 550, 584
BciT130I CCWGG 1 cut(s) 652
BclI TGATCA 1 cut(s) 9
BcoDI GTCTC 1 cut(s) 87
BcuI ACTAGT 1 cut(s) 443
BfaI CTAG 3 cut(s) 227, 444, 671
BfmI CTRYAG 1 cut(s) 750
BfuAI ACCTGC 1 cut(s) 621
BglI GCCNNNNNGGC 2 cut(s) 292, 1103
BisI GCNGC 8 cut(s) 99, 102, 338, 423, 426, 536, 543, 982
BlnI CCTAGG 1 cut(s) 226
BlsI GCNGC 8 cut(s) 100, 103, 339, 424, 427, 537, 544, 983
Bme1390I CCNGG 1 cut(s) 652
Bme18I GGWCC 1 cut(s) 1066
BmgT120I GGNCC 1 cut(s) 1066
BmiI GGNNCC 3 cut(s) 214, 922, 1067
BmrFI CCNGG 1 cut(s) 652
BmrI ACTGGG 3 cut(s) 300, 311, 354
BmsI GCATC 3 cut(s) 683, 964, 1181
BmuI ACTGGG 3 cut(s) 300, 311, 354
BpmI CTGGAG 1 cut(s) 673
BpuEI CTTGAG 1 cut(s) 69
BsaBI GATNNNNATC 1 cut(s) 507
BsaJI CCNNGG 4 cut(s) 226, 650, 916, 924
BsaXI ACNNNNNCTCC 2 cut(s) 58, 88
Bsc4I CCNNNNNNNGG 2 cut(s) 294, 752
Bse1I ACTGG 3 cut(s) 295, 317, 360
Bse3DI GCAATG 1 cut(s) 332
Bse8I GATNNNNATC 1 cut(s) 507
BseBI CCWGG 1 cut(s) 652
BseDI CCNNGG 4 cut(s) 226, 650, 916, 924
BseGI GGATG 4 cut(s) 674, 799, 1075, 1168
BseJI GATNNNNATC 1 cut(s) 507
BseLI CCNNNNNNNGG 2 cut(s) 294, 752
BseMI GCAATG 1 cut(s) 332
BseNI ACTGG 3 cut(s) 295, 317, 360
BseRI GAGGAG 5 cut(s) 114, 153, 187, 753, 824
BseX3I CGGCCG 1 cut(s) 262
BseXI GCAGC 5 cut(s) 409, 412, 529, 547, 968
BsgI GTGCAG 1 cut(s) 1112
Bsh1236I CGCG 1 cut(s) 243
Bsh1285I CGRYCG 2 cut(s) 250, 265
BshFI GGCC 2 cut(s) 264, 1125
BshNI GGYRCC 1 cut(s) 212
BsiEI CGRYCG 2 cut(s) 250, 265
BsiHKAI GWGCWC 2 cut(s) 573, 763
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 3 cut(s) 155, 244, 1079
BslI CCNNNNNNNGG 2 cut(s) 294, 752
BsmAI GTCTC 1 cut(s) 87
BsmBI CGTCTC 1 cut(s) 87
BsmFI GGGAC 3 cut(s) 155, 244, 1079
BsnI GGCC 2 cut(s) 264, 1125
Bsp1286I GDGCHC 2 cut(s) 573, 763
Bsp1407I TGTACA 1 cut(s) 705
Bsp143I GATC 3 cut(s) 9, 550, 920
Bsp19I CCATGG 1 cut(s) 924
BspANI GGCC 2 cut(s) 264, 1125
BspFNI CGCG 1 cut(s) 243
BspHI TCATGA 1 cut(s) 846
BspLI GGNNCC 3 cut(s) 214, 922, 1067
BspMI ACCTGC 1 cut(s) 621
BspPI GGATC 2 cut(s) 915, 928
BspQI GCTCTTC 1 cut(s) 870
BspT107I GGYRCC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 332
BsrGI TGTACA 1 cut(s) 705
BsrI ACTGG 3 cut(s) 295, 317, 360
BssECI CCNNGG 4 cut(s) 226, 650, 916, 924
BssMI GATC 3 cut(s) 9, 550, 920
BssT1I CCWWGG 3 cut(s) 226, 916, 924
Bst2UI CCWGG 1 cut(s) 652
Bst4CI ACNGT 3 cut(s) 682, 956, 1090
Bst6I CTCTTC 5 cut(s) 38, 132, 185, 376, 870
BstAUI TGTACA 1 cut(s) 705
BstC8I GCNNGC 5 cut(s) 241, 540, 979, 1036, 1127
BstDSI CCRYGG 1 cut(s) 924
BstF5I GGATG 4 cut(s) 674, 799, 1075, 1168
BstFNI CGCG 1 cut(s) 243
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 3 cut(s) 12, 553, 923
BstMAI GTCTC 1 cut(s) 87
BstMBI GATC 3 cut(s) 9, 550, 920
BstMCI CGRYCG 2 cut(s) 250, 265
BstMWI GCNNNNNNNGC 5 cut(s) 249, 292, 343, 422, 1103
BstNI CCWGG 1 cut(s) 652
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 1 cut(s) 650
BstSFI CTRYAG 1 cut(s) 750
BstUI CGCG 1 cut(s) 243
BstV1I GCAGC 5 cut(s) 409, 412, 529, 547, 968
BstX2I RGATCY 1 cut(s) 920
BstXI CCANNNNNNTGG 1 cut(s) 290
BstYI RGATCY 1 cut(s) 920
BstZI CGGCCG 1 cut(s) 262
BsuRI GGCC 2 cut(s) 264, 1125
BtgI CCRYGG 1 cut(s) 924
BtgZI GCGATG 2 cut(s) 20, 79
BtsCI GGATG 4 cut(s) 674, 799, 1075, 1168
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 3 cut(s) 69, 288, 678
BveI ACCTGC 1 cut(s) 621
Cac8I GCNNGC 5 cut(s) 241, 540, 979, 1036, 1127
CciI TCATGA 1 cut(s) 846
CfoI GCGC 1 cut(s) 245
Cfr13I GGNCC 1 cut(s) 1066
Csp6I GTAC 3 cut(s) 356, 706, 781
CviAII CATG 7 cut(s) 401, 467, 823, 847, 925, 1012, 1030
CviQI GTAC 3 cut(s) 356, 706, 781
DpnI GATC 3 cut(s) 11, 552, 922
DpnII GATC 3 cut(s) 9, 550, 920
DraI TTTAAA 1 cut(s) 961
DrdI GACNNNNNNGTC 1 cut(s) 200
DseDI GACNNNNNNGTC 1 cut(s) 200
EaeI YGGCCR 1 cut(s) 262
EagI CGGCCG 1 cut(s) 262
Eam1104I CTCTTC 5 cut(s) 38, 132, 185, 376, 870
EarI CTCTTC 5 cut(s) 38, 132, 185, 376, 870
EciI GGCGGA 1 cut(s) 66
EclXI CGGCCG 1 cut(s) 262
Eco130I CCWWGG 3 cut(s) 226, 916, 924
Eco47I GGWCC 1 cut(s) 1066
Eco52I CGGCCG 1 cut(s) 262
EcoRI GAATTC 1 cut(s) 562
EcoRII CCWGG 1 cut(s) 650
EcoT14I CCWWGG 3 cut(s) 226, 916, 924
ErhI CCWWGG 3 cut(s) 226, 916, 924
Esp3I CGTCTC 1 cut(s) 87
FaeI CATG 7 cut(s) 404, 470, 826, 850, 928, 1015, 1033
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FaqI GGGAC 3 cut(s) 155, 244, 1079
FatI CATG 7 cut(s) 400, 466, 822, 846, 924, 1011, 1029
FbaI TGATCA 1 cut(s) 9
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 8 cut(s) 99, 102, 338, 423, 426, 536, 543, 982
FokI GGATG 4 cut(s) 661, 806, 1062, 1175
Fsp4HI GCNGC 8 cut(s) 99, 102, 338, 423, 426, 536, 543, 982
FspBI CTAG 3 cut(s) 227, 444, 671
GlaI GCGC 1 cut(s) 244
GluI GCNGC 8 cut(s) 99, 102, 338, 423, 426, 536, 543, 982
GsuI CTGGAG 1 cut(s) 673
HaeIII GGCC 2 cut(s) 264, 1125
HapII CCGG 1 cut(s) 210
HhaI GCGC 1 cut(s) 245
Hin1II CATG 7 cut(s) 404, 470, 826, 850, 928, 1015, 1033
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HincII GTYRAC 2 cut(s) 205, 1093
HindII GTYRAC 2 cut(s) 205, 1093
HinfI GANTC 6 cut(s) 160, 194, 206, 312, 487, 623
HpaI GTTAAC 1 cut(s) 1093
HpaII CCGG 1 cut(s) 210
HphI GGTGA 4 cut(s) 34, 958, 1004, 1014
Hpy166II GTNNAC 3 cut(s) 205, 498, 1093
Hpy188I TCNGA 4 cut(s) 454, 946, 1051, 1149
Hpy188III TCNNGA 6 cut(s) 86, 559, 697, 721, 847, 1117
Hpy8I GTNNAC 3 cut(s) 205, 498, 1093
Hpy99I CGWCG 2 cut(s) 56, 206
HpyAV CCTTC 2 cut(s) 718, 784
HpyCH4III ACNGT 3 cut(s) 682, 956, 1090
HpyCH4IV ACGT 1 cut(s) 630
HpyCH4V TGCA 5 cut(s) 303, 332, 634, 1038, 1129
HpyF10VI GCNNNNNNNGC 5 cut(s) 249, 292, 343, 422, 1103
HpySE526I ACGT 1 cut(s) 630
Hsp92II CATG 7 cut(s) 404, 470, 826, 850, 928, 1015, 1033
HspAI GCGC 1 cut(s) 243
Ksp22I TGATCA 1 cut(s) 9
KspAI GTTAAC 1 cut(s) 1093
Kzo9I GATC 3 cut(s) 9, 550, 920
LguI GCTCTTC 1 cut(s) 870
LmnI GCTCC 2 cut(s) 79, 766
Lsp1109I GCAGC 5 cut(s) 409, 412, 529, 547, 968
LweI GCATC 3 cut(s) 683, 964, 1181
MaeI CTAG 3 cut(s) 227, 444, 671
MaeII ACGT 1 cut(s) 630
MaeIII GTNAC 3 cut(s) 266, 964, 992
MalI GATC 3 cut(s) 11, 552, 922
MboI GATC 3 cut(s) 9, 550, 920
MflI RGATCY 1 cut(s) 920
MhlI GDGCHC 2 cut(s) 573, 763
MluCI AATT 4 cut(s) 432, 455, 562, 1070
MlyI GAGTC 4 cut(s) 154, 188, 200, 632
MmeI TCCRAC 2 cut(s) 651, 1103
MroXI GAANNNNTTC 1 cut(s) 879
MseI TTAA 4 cut(s) 714, 935, 960, 1092
MslI CAYNNNNRTG 3 cut(s) 827, 929, 1136
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 652
MvaI CCWGG 1 cut(s) 652
MvnI CGCG 1 cut(s) 243
MwoI GCNNNNNNNGC 5 cut(s) 249, 292, 343, 422, 1103
NcoI CCATGG 1 cut(s) 924
NdeII GATC 3 cut(s) 9, 550, 920
NlaIII CATG 7 cut(s) 404, 470, 826, 850, 928, 1015, 1033
NlaIV GGNNCC 3 cut(s) 214, 922, 1067
NmuCI GTSAC 3 cut(s) 266, 964, 992
NspI RCATGY 1 cut(s) 470
PagI TCATGA 1 cut(s) 846
PciI ACATGT 1 cut(s) 466
PciSI GCTCTTC 1 cut(s) 870
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 243
PdmI GAANNNNTTC 1 cut(s) 879
PfeI GAWTC 2 cut(s) 312, 487
PkrI GCNGC 8 cut(s) 100, 103, 339, 424, 427, 537, 544, 983
PleI GAGTC 4 cut(s) 154, 188, 200, 631
PpsI GAGTC 4 cut(s) 154, 188, 200, 631
PscI ACATGT 1 cut(s) 466
PshBI ATTAAT 1 cut(s) 714
Psp6I CCWGG 1 cut(s) 650
PspGI CCWGG 1 cut(s) 650
PspN4I GGNNCC 3 cut(s) 214, 922, 1067
PspPI GGNCC 1 cut(s) 1066
PsuI RGATCY 1 cut(s) 920
RsaI GTAC 3 cut(s) 357, 707, 782
RsaNI GTAC 3 cut(s) 356, 706, 781
RseI CAYNNNNRTG 3 cut(s) 827, 929, 1136
SalI GTCGAC 1 cut(s) 203
SapI GCTCTTC 1 cut(s) 870
SaqAI TTAA 4 cut(s) 714, 935, 960, 1092
SatI GCNGC 8 cut(s) 99, 102, 338, 423, 426, 536, 543, 982
Sau3AI GATC 3 cut(s) 9, 550, 920
Sau96I GGNCC 1 cut(s) 1066
SchI GAGTC 4 cut(s) 154, 188, 200, 632
ScrFI CCNGG 1 cut(s) 652
SduI GDGCHC 2 cut(s) 573, 763
SetI ASST 9 cut(s) 544, 615, 633, 729, 751, 774, 854, 870, 882
SfaNI GCATC 3 cut(s) 683, 964, 1181
SfcI CTRYAG 1 cut(s) 750
SinI GGWCC 1 cut(s) 1066
SmiMI CAYNNNNRTG 3 cut(s) 827, 929, 1136
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
SpeI ACTAGT 1 cut(s) 443
Sse9I AATT 4 cut(s) 432, 455, 562, 1070
SspMI CTAG 3 cut(s) 227, 444, 671
StyD4I CCNGG 1 cut(s) 650
StyI CCWWGG 3 cut(s) 226, 916, 924
TaaI ACNGT 3 cut(s) 682, 956, 1090
TaiI ACGT 1 cut(s) 633
TaqI TCGA 6 cut(s) 204, 273, 321, 560, 581, 1175
TasI AATT 4 cut(s) 432, 455, 562, 1070
TatI WGTACW 1 cut(s) 705
TauI GCSGC 3 cut(s) 101, 104, 340
TfiI GAWTC 2 cut(s) 312, 487
Tru1I TTAA 4 cut(s) 714, 935, 960, 1092
Tru9I TTAA 4 cut(s) 714, 935, 960, 1092
TscAI CASTG 3 cut(s) 76, 295, 685
TseFI GTSAC 3 cut(s) 266, 964, 992
TseI GCWGC 5 cut(s) 422, 425, 535, 542, 981
Tsp45I GTSAC 3 cut(s) 266, 964, 992
TspDTI ATGAA 3 cut(s) 389, 888, 1166
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 3 cut(s) 76, 295, 685
VpaK11BI GGWCC 1 cut(s) 1066
VspI ATTAAT 1 cut(s) 714
XapI RAATTY 1 cut(s) 562
XceI RCATGY 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 287
XmaJI CCTAGG 1 cut(s) 226
XmiI GTMKAC 1 cut(s) 204
XmnI GAANNNNTTC 1 cut(s) 879
XspI CTAG 3 cut(s) 227, 444, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.