MD06G1109100.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014251
Physical Location & Seq
Forward (+)
24855558 .. 24857452
1895 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1109100.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGGCGATCAAGCTTCTTGTTTCTTTGCTCTGCATTTTCATCTCCACCGCCGTTTCGCAACCGTATTCTACTCCGTATTCCCAAGAACTCATCAATTCCATCAGCCCGATTCTCTCACCTTCTTCTTCTTCATTCCCTCCGGCCCCAACAGCTTCAACCGAGTCATTTCAGACGACTCCCCTCGTCCCGGCGCTCTTCATCATCGGAGACTCCTCCGTCGACTGTGGCACCAACAATTTCCTCGGAACCTTTGCCCGCGCCGACCGCCTCCCCTACGGTCGAGACTTTGATACCCACCAACCCACTGGGAGGTTCTGCAATGGCAGAATCCCTGTCGATTATCTTGCGCTCCGTCTCGGGCTTCCTTTCGTACCAAGTTATCTTGGGCAGAACGGAAGAGTGGAAGAGATGATTCACGGATTGAATTTTGCGTCTGCCGGTGCCGGAATTATATTCACCAGTGGCTCAGAATTGGGACAGCATGTTTCTTTCACTCAGCAAGTTCAGCAGTTCACAGACACCTATCAGCATTTTATATTAAGTTTGGGAGAGCAAGCTGCAATTGATCTCGTCTCAAATTCGGTGCTTTACATTTCGATTGGAATCAACGACTACATACATTACTATTTGCGTAACGTATCCAACGTGCAAAATCTGTATCTGCCCTGGGGTTTCAATCAGTTTTTAGCATCCACATTGAGGCAGGAAATTAAGAACTTGTACAACATAAATGTGAAGAAGGTGGTAGTGATGGGGCTGGCACCGATTGGCTGTGCGCCTCACTATTTATTTCAGTACAGAGTGAAGGATGGTCAATGTATTGAGGAGATAAACAACATGGTAATGGAGTTCAACTTTGTGATGAGGTATATGATCGAGGAGCTACGTGAAGAGCTTCCCGCTTCCAACATCATCTTCTGCGATGTGTTTGAAGGTTCCATTGATATTATAAAGAACTTTGCACGTTACGGTTTCAATGAAACTACTGATGCCTGCTGTGGGTTGGGGGAGCACAAGGGTTTTGTCATGTGTTTATCGCCAGATATGGCGTGCAATGATGCTTCTAATCATGTCTGGTGGGATCAGTTCCATCCAACGGATGCAGTCAATGCCATCCTAGCTGATAATGTGTGGAATGGCCTGCATGCGAAAATGTGCTATCCGATGAACTTAGAGGATATGGTAGTATCGAGCTCCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

401

Amino Acids

44.64

Weight (kDa)

4.92

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 65 - 376 4.5e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10950 AT4G10950
fragaria_vesca FvH4_5g04800
malus_domestica MD06G1109100.v1.1
prunus_persica Prupe.5G122000_v2.0.a1
pyrus_communis pycom06g10240
rosa_chinensis RchiOBHm_Chr7g0194451
rosa_laevigata RLG00000004200
rosa_multiflora Rmu_sc0030308.1_g000001 Rmu_ssc0000261.1_g000027
rosa_roxburghii Rroxscaffold_3G00261350
rosa_rugosa Rorug07G0015700
rosa_samantha Rh7AG143000 Rh7BG143500 Rh7CG147300 Rh7DG144900
rosa_wichuraiana Rw7G012240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 950
AasI GACNNNNNNGTC 1 cut(s) 215
AccB1I GGYRCC 3 cut(s) 227, 440, 760
AccI GTMKAC 1 cut(s) 219
AccII CGCG 1 cut(s) 258
AciI CCGC 4 cut(s) 48, 256, 265, 900
AclWI GGATC 1 cut(s) 1089
AcsI RAATTY 2 cut(s) 424, 577
AfaI GTAC 3 cut(s) 372, 722, 797
AfiI CCNNNNNNNGG 5 cut(s) 187, 309, 699, 999, 1096
AgsI TTSAA 6 cut(s) 156, 424, 676, 853, 932, 976
AjnI CCWGG 1 cut(s) 665
AjuI GAANNNNNNNTTGG 2 cut(s) 899, 931
AluBI AGCT 7 cut(s) 13, 152, 557, 883, 895, 1121, 1194
AluI AGCT 7 cut(s) 13, 152, 557, 883, 895, 1121, 1194
Alw21I GWGCWC 2 cut(s) 1014, 1196
Alw26I GTCTC 4 cut(s) 201, 276, 359, 577
AlwI GGATC 1 cut(s) 1089
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 2 cut(s) 141, 1138
ApeKI GCWGC 1 cut(s) 557
ApoI RAATTY 2 cut(s) 424, 577
Asp700I GAANNNNTTC 1 cut(s) 894
AspLEI GCGC 4 cut(s) 193, 260, 349, 778
AspS9I GGNCC 1 cut(s) 142
AsuC2I CCSGG 1 cut(s) 188
AsuHPI GGTGA 2 cut(s) 108, 448
AvaI CYCGRG 1 cut(s) 356
BanI GGYRCC 3 cut(s) 227, 440, 760
BanII GRGCYC 1 cut(s) 1196
Bbv12I GWGCWC 2 cut(s) 1014, 1196
BbvI GCAGC 1 cut(s) 544
BccI CCATC 5 cut(s) 107, 745, 803, 1098, 1121
BceAI ACGGC 1 cut(s) 35
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BciT130I CCWGG 1 cut(s) 667
BciVI GTATCC 1 cut(s) 649
BcnI CCSGG 1 cut(s) 188
BcoDI GTCTC 4 cut(s) 201, 276, 359, 577
BfaI CTAG 1 cut(s) 1118
BfoI RGCGCY 1 cut(s) 194
BfuI GTATCC 1 cut(s) 649
BisI GCNGC 1 cut(s) 558
BlsI GCNGC 1 cut(s) 559
Bme1390I CCNGG 2 cut(s) 188, 667
BmeT110I CYCGRG 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 142
BmiI GGNNCC 6 cut(s) 144, 229, 247, 442, 762, 937
BmrFI CCNGG 2 cut(s) 188, 667
BmrI ACTGGG 1 cut(s) 315
BmsI GCATC 4 cut(s) 698, 979, 1048, 1090
BmuI ACTGGG 1 cut(s) 315
BpuMI CCSGG 1 cut(s) 188
BsaAI YACGTR 1 cut(s) 887
BsaBI GATNNNNATC 1 cut(s) 602
BsaJI CCNNGG 3 cut(s) 241, 665, 666
Bsc4I CCNNNNNNNGG 5 cut(s) 187, 309, 699, 999, 1096
Bse118I RCCGGY 1 cut(s) 437
Bse1I ACTGG 2 cut(s) 310, 459
Bse3DI GCAATG 2 cut(s) 325, 1060
Bse8I GATNNNNATC 1 cut(s) 602
BseBI CCWGG 1 cut(s) 667
BseDI CCNNGG 3 cut(s) 241, 665, 666
BseGI GGATG 5 cut(s) 689, 814, 1090, 1105, 1113
BseJI GATNNNNATC 1 cut(s) 602
BseLI CCNNNNNNNGG 5 cut(s) 187, 309, 699, 999, 1096
BseMI GCAATG 2 cut(s) 325, 1060
BseMII CTCAG 2 cut(s) 480, 509
BseNI ACTGG 2 cut(s) 310, 459
BseRI GAGGAG 3 cut(s) 202, 839, 893
BseXI GCAGC 1 cut(s) 544
Bsh1236I CGCG 1 cut(s) 258
Bsh1285I CGRYCG 2 cut(s) 265, 280
BshFI GGCC 2 cut(s) 143, 1140
BshNI GGYRCC 3 cut(s) 227, 440, 760
BsiEI CGRYCG 2 cut(s) 265, 280
BsiHKAI GWGCWC 2 cut(s) 1014, 1196
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 4 cut(s) 140, 188, 438, 444
BslFI GGGAC 2 cut(s) 170, 489
BslI CCNNNNNNNGG 5 cut(s) 187, 309, 699, 999, 1096
BsmAI GTCTC 4 cut(s) 201, 276, 359, 577
BsmBI CGTCTC 2 cut(s) 359, 577
BsmFI GGGAC 2 cut(s) 170, 489
BsnI GGCC 2 cut(s) 143, 1140
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 2 cut(s) 1014, 1196
Bsp1407I TGTACA 1 cut(s) 720
Bsp143I GATC 4 cut(s) 6, 565, 873, 1081
BspACI CCGC 4 cut(s) 48, 256, 265, 900
BspANI GGCC 2 cut(s) 143, 1140
BspCNI CTCAG 2 cut(s) 479, 508
BspFNI CGCG 1 cut(s) 258
BspLI GGNNCC 6 cut(s) 144, 229, 247, 442, 762, 937
BspPI GGATC 1 cut(s) 1089
BspQI GCTCTTC 2 cut(s) 200, 885
BspT107I GGYRCC 3 cut(s) 227, 440, 760
BsrDI GCAATG 2 cut(s) 325, 1060
BsrFI RCCGGY 1 cut(s) 437
BsrGI TGTACA 1 cut(s) 720
BsrI ACTGG 2 cut(s) 310, 459
BssAI RCCGGY 1 cut(s) 437
BssECI CCNNGG 3 cut(s) 241, 665, 666
BssMI GATC 4 cut(s) 6, 565, 873, 1081
Bst2UI CCWGG 1 cut(s) 667
Bst4CI ACNGT 4 cut(s) 63, 224, 278, 971
Bst6I CTCTTC 4 cut(s) 200, 391, 399, 885
BstAPI GCANNNNNTGC 1 cut(s) 1109
BstAUI TGTACA 1 cut(s) 720
BstBAI YACGTR 1 cut(s) 887
BstC8I GCNNGC 7 cut(s) 256, 555, 759, 994, 1051, 1142, 1146
BstDEI CTNAG 3 cut(s) 466, 495, 1171
BstF5I GGATG 5 cut(s) 689, 814, 1090, 1105, 1113
BstFNI CGCG 1 cut(s) 258
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 4 cut(s) 193, 260, 349, 778
BstKTI GATC 4 cut(s) 9, 568, 876, 1084
BstMAI GTCTC 4 cut(s) 201, 276, 359, 577
BstMBI GATC 4 cut(s) 6, 565, 873, 1081
BstMCI CGRYCG 2 cut(s) 265, 280
BstMWI GCNNNNNNNGC 5 cut(s) 149, 264, 505, 1109, 1118
BstNI CCWGG 1 cut(s) 667
BstNSI RCATGY 2 cut(s) 485, 1148
BstSCI CCNGG 2 cut(s) 186, 665
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 544
BstXI CCANNNNNNTGG 1 cut(s) 305
BsuI GTATCC 1 cut(s) 649
BsuRI GGCC 2 cut(s) 143, 1140
BtgZI GCGATG 1 cut(s) 936
BtsCI GGATG 5 cut(s) 689, 814, 1090, 1105, 1113
BtsIMutI CAGTG 2 cut(s) 303, 466
Cac8I GCNNGC 7 cut(s) 256, 555, 759, 994, 1051, 1142, 1146
CfoI GCGC 4 cut(s) 193, 260, 349, 778
Cfr10I RCCGGY 1 cut(s) 437
Cfr13I GGNCC 1 cut(s) 142
CseI GACGC 1 cut(s) 420
Csp6I GTAC 3 cut(s) 371, 721, 796
CviAII CATG 5 cut(s) 482, 838, 1027, 1070, 1145
CviQI GTAC 3 cut(s) 371, 721, 796
DdeI CTNAG 3 cut(s) 466, 495, 1171
DpnI GATC 4 cut(s) 8, 567, 875, 1083
DpnII GATC 4 cut(s) 6, 565, 873, 1081
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
Eam1104I CTCTTC 4 cut(s) 200, 391, 399, 885
EarI CTCTTC 4 cut(s) 200, 391, 399, 885
Ecl136II GAGCTC 1 cut(s) 1194
Eco24I GRGCYC 1 cut(s) 1196
Eco53kI GAGCTC 1 cut(s) 1194
Eco88I CYCGRG 1 cut(s) 356
EcoICRI GAGCTC 1 cut(s) 1194
EcoRII CCWGG 1 cut(s) 665
EcoT38I GRGCYC 1 cut(s) 1196
Esp3I CGTCTC 2 cut(s) 359, 577
FaeI CATG 5 cut(s) 485, 841, 1030, 1073, 1148
FaqI GGGAC 2 cut(s) 170, 489
FatI CATG 5 cut(s) 481, 837, 1026, 1069, 1144
FauI CCCGC 2 cut(s) 263, 907
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 1 cut(s) 558
FokI GGATG 5 cut(s) 676, 821, 1077, 1100, 1112
FriOI GRGCYC 1 cut(s) 1196
Fsp4HI GCNGC 1 cut(s) 558
FspBI CTAG 1 cut(s) 1118
GlaI GCGC 4 cut(s) 192, 259, 348, 777
GluI GCNGC 1 cut(s) 558
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 2 cut(s) 143, 1140
HapII CCGG 4 cut(s) 140, 188, 438, 444
HgaI GACGC 1 cut(s) 420
HhaI GCGC 4 cut(s) 193, 260, 349, 778
Hin1II CATG 5 cut(s) 485, 841, 1030, 1073, 1148
Hin6I GCGC 4 cut(s) 191, 258, 347, 776
HinP1I GCGC 4 cut(s) 191, 258, 347, 776
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 7 cut(s) 109, 161, 175, 209, 327, 412, 603
HpaII CCGG 4 cut(s) 140, 188, 438, 444
HphI GGTGA 2 cut(s) 108, 448
Hpy166II GTNNAC 2 cut(s) 220, 513
Hpy188I TCNGA 5 cut(s) 171, 206, 245, 469, 1164
Hpy188III TCNNGA 1 cut(s) 281
Hpy8I GTNNAC 2 cut(s) 220, 513
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 4 cut(s) 129, 733, 799, 926
HpyCH4III ACNGT 4 cut(s) 63, 224, 278, 971
HpyCH4IV ACGT 4 cut(s) 636, 645, 886, 964
HpyCH4V TGCA 8 cut(s) 33, 318, 560, 649, 962, 1053, 1103, 1144
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 264, 505, 1109, 1118
HpyF3I CTNAG 3 cut(s) 466, 495, 1171
HpySE526I ACGT 4 cut(s) 636, 645, 886, 964
Hsp92II CATG 5 cut(s) 485, 841, 1030, 1073, 1148
HspAI GCGC 4 cut(s) 191, 258, 347, 776
Kzo9I GATC 4 cut(s) 6, 565, 873, 1081
LguI GCTCTTC 2 cut(s) 200, 885
LmnI GCTCC 4 cut(s) 354, 880, 1009, 1199
Lsp1109I GCAGC 1 cut(s) 544
LweI GCATC 4 cut(s) 698, 979, 1048, 1090
MaeI CTAG 1 cut(s) 1118
MaeII ACGT 4 cut(s) 636, 645, 886, 964
MaeIII GTNAC 2 cut(s) 632, 965
MalI GATC 4 cut(s) 8, 567, 875, 1083
MboI GATC 4 cut(s) 6, 565, 873, 1081
MboII GAAGA 9 cut(s) 114, 117, 120, 187, 408, 416, 748, 902, 907
MfeI CAATTG 1 cut(s) 561
MhlI GDGCHC 2 cut(s) 1014, 1196
MluCI AATT 8 cut(s) 94, 235, 424, 447, 470, 561, 577, 708
MlyI GAGTC 3 cut(s) 169, 170, 203
MmeI TCCRAC 3 cut(s) 666, 930, 1118
MroXI GAANNNNTTC 1 cut(s) 894
MseI TTAA 2 cut(s) 539, 711
MslI CAYNNNNRTG 2 cut(s) 731, 842
MspI CCGG 4 cut(s) 140, 188, 438, 444
MspR9I CCNGG 2 cut(s) 188, 667
MunI CAATTG 1 cut(s) 561
MvaI CCWGG 1 cut(s) 667
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 5 cut(s) 149, 264, 505, 1109, 1118
NciI CCSGG 1 cut(s) 188
NdeII GATC 4 cut(s) 6, 565, 873, 1081
NlaIII CATG 5 cut(s) 485, 841, 1030, 1073, 1148
NlaIV GGNNCC 6 cut(s) 144, 229, 247, 442, 762, 937
NspI RCATGY 2 cut(s) 485, 1148
PaeI GCATGC 1 cut(s) 1148
PasI CCCWGGG 1 cut(s) 666
PciSI GCTCTTC 2 cut(s) 200, 885
PcsI WCGNNNNNNNCGW 1 cut(s) 642
PdmI GAANNNNTTC 1 cut(s) 894
PfeI GAWTC 4 cut(s) 109, 327, 412, 603
PkrI GCNGC 1 cut(s) 559
PleI GAGTC 3 cut(s) 169, 169, 203
PpsI GAGTC 3 cut(s) 169, 169, 203
Ppu21I YACGTR 1 cut(s) 887
PsiI TTATAA 1 cut(s) 950
Psp124BI GAGCTC 1 cut(s) 1196
Psp6I CCWGG 1 cut(s) 665
PspGI CCWGG 1 cut(s) 665
PspN4I GGNNCC 6 cut(s) 144, 229, 247, 442, 762, 937
PspPI GGNCC 1 cut(s) 142
RsaI GTAC 3 cut(s) 372, 722, 797
RsaNI GTAC 3 cut(s) 371, 721, 796
RseI CAYNNNNRTG 2 cut(s) 731, 842
SacI GAGCTC 1 cut(s) 1196
SalI GTCGAC 1 cut(s) 218
SapI GCTCTTC 2 cut(s) 200, 885
SaqAI TTAA 2 cut(s) 539, 711
SatI GCNGC 1 cut(s) 558
Sau3AI GATC 4 cut(s) 6, 565, 873, 1081
Sau96I GGNCC 1 cut(s) 142
SchI GAGTC 3 cut(s) 169, 170, 203
ScrFI CCNGG 2 cut(s) 188, 667
SduI GDGCHC 2 cut(s) 1014, 1196
SfaNI GCATC 4 cut(s) 698, 979, 1048, 1090
SmiMI CAYNNNNRTG 2 cut(s) 731, 842
SphI GCATGC 1 cut(s) 1148
Sse9I AATT 8 cut(s) 94, 235, 424, 447, 470, 561, 577, 708
SsiI CCGC 4 cut(s) 48, 256, 265, 900
SspMI CTAG 1 cut(s) 1118
SstI GAGCTC 1 cut(s) 1196
StyD4I CCNGG 2 cut(s) 186, 665
TaaI ACNGT 4 cut(s) 63, 224, 278, 971
TaiI ACGT 4 cut(s) 639, 648, 889, 967
TaqI TCGA 6 cut(s) 219, 280, 336, 596, 876, 1190
TasI AATT 8 cut(s) 94, 235, 424, 447, 470, 561, 577, 708
TatI WGTACW 2 cut(s) 720, 795
TfiI GAWTC 4 cut(s) 109, 327, 412, 603
Tru1I TTAA 2 cut(s) 539, 711
Tru9I TTAA 2 cut(s) 539, 711
TscAI CASTG 2 cut(s) 310, 466
TseI GCWGC 1 cut(s) 557
TspDTI ATGAA 5 cut(s) 28, 120, 187, 993, 1181
TspGWI ACGGA 6 cut(s) 63, 205, 341, 408, 432, 1112
TspRI CASTG 2 cut(s) 310, 466
XapI RAATTY 2 cut(s) 424, 577
XceI RCATGY 2 cut(s) 485, 1148
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 219
XmnI GAANNNNTTC 1 cut(s) 894
XspI CTAG 1 cut(s) 1118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.