Rmu_ssc0000261.1_g000027

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000261.1
OG0014251
Physical Location & Seq
Forward (+)
182736 .. 184702
1967 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000261.1_g000027.1.cds

Sequence Viewer

Length: 1203 bp
atggcgacgatcaatcttcttctctcttttctctgcattctcaccaccaccgccctttcacaaccaaccattgctccgccgtactctcaagaacccatcgatgccgccacccccatctctccagcctcttcttctcctccttcaatctccccaaccccaccaccggcctcaatcccacccaagactcctctcgtccctgccctcttcgtcatcggagactcctccgtcgactccggcaccaacaatttcctcgggactttcgcacgagccgaccgcctcccctacggccgcgatttcgacacgcacgagcccactgggcagttctgcaatggtagaatcccagtcgattatattgcattgcggcttgggcttccttttgtacccagttttcttggccagactggaagagtggaagatatgcttcatggattgaactttgcttctgctgctgctggaattatattcaccagtgggtcagaattgggtcaacatgtttccttgtctcagcaaattcagcagtttacagatacttacgagcagtttgtgttgagtatggggcagcaagctgcgattaatctcatctcgaattcggtgctctacatttcaatcggaatcaatgactacatacattactacttgcgtaatgagtcgaacgtgcaaaatctctatttgccctggagtttcaatcgatttctagcatccacggtgacgcaggaaatcaaggtgagaattaatgtcaggaaggtggttgtgatgggtctgccacctataggttgtgctcctcactacttatgtgagtacaaagtgaaggatggacaatgtgttgaggagataaacaacatggtattggagttcaactttatcatgaggtatatgattgaagagctaagtgaagcgcttccgtattcccatatcatcttctgtgatgtgttccaaggatccatggatatgttaaagaactctgaacgttacggtttcaaagtcaccaccgatgcttgctgcggattaggcgactacaagggttttctcatgtgcttatcaccagacatggcttgcagtaatgcatctgaccatatctggtgggaccaattccatccaactgatgcagttaatgccattctggctgataatgtctggaatggcctgcacacgaaaatgtgctatccaatgaacttggaggatgtggtagccaatacatactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

400

Amino Acids

44.27

Weight (kDa)

4.84

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10950 AT4G10950
fragaria_vesca FvH4_5g04800
malus_domestica MD06G1109100.v1.1
prunus_persica Prupe.5G122000_v2.0.a1
pyrus_communis pycom06g10240
rosa_chinensis RchiOBHm_Chr7g0194451
rosa_laevigata RLG00000004200
rosa_multiflora Rmu_sc0030308.1_g000001 Rmu_ssc0000261.1_g000027
rosa_roxburghii Rroxscaffold_3G00261350
rosa_rugosa Rorug07G0015700
rosa_samantha Rh7AG143000 Rh7BG143500 Rh7CG147300 Rh7DG144900
rosa_wichuraiana Rw7G012240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 224
AccB1I GGYRCC 1 cut(s) 236
AccI GTMKAC 1 cut(s) 228
AccII CGCG 1 cut(s) 291
AciI CCGC 7 cut(s) 51, 77, 105, 274, 289, 361, 1002
AclI AACGTT 1 cut(s) 967
AclWI GGATC 2 cut(s) 933, 946
AcoI YGGCCR 2 cut(s) 286, 394
AcsI RAATTY 2 cut(s) 510, 586
AfaI GTAC 3 cut(s) 83, 381, 800
AfeI AGCGCT 1 cut(s) 897
AfiI CCNNNNNNNGG 2 cut(s) 163, 770
AflIII ACRYGT 1 cut(s) 490
AgsI TTSAA 7 cut(s) 144, 433, 606, 685, 856, 881, 979
AjnI CCWGG 1 cut(s) 674
AluBI AGCT 2 cut(s) 566, 886
AluI AGCT 2 cut(s) 566, 886
Alw21I GWGCWC 2 cut(s) 597, 781
Alw26I GTCTC 2 cut(s) 210, 507
AlwI GGATC 2 cut(s) 933, 946
Ama87I CYCGRG 1 cut(s) 251
Aor51HI AGCGCT 1 cut(s) 897
AoxI GGCC 4 cut(s) 165, 286, 394, 1141
ApeKI GCWGC 5 cut(s) 446, 449, 559, 566, 999
ApoI RAATTY 2 cut(s) 510, 586
AseI ATTAAT 2 cut(s) 573, 732
Asp700I GAANNNNTTC 1 cut(s) 897
AspLEI GCGC 1 cut(s) 898
AspS9I GGNCC 1 cut(s) 1084
AsuHPI GGTGA 6 cut(s) 34, 457, 718, 736, 976, 1032
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 1 cut(s) 1084
BalI TGGCCA 1 cut(s) 396
BamHI GGATCC 1 cut(s) 938
BanI GGYRCC 1 cut(s) 236
BanII GRGCYC 1 cut(s) 312
BauI CACGAG 2 cut(s) 264, 305
Bbv12I GWGCWC 2 cut(s) 597, 781
BbvI GCAGC 5 cut(s) 433, 436, 553, 571, 986
BccI CCATC 5 cut(s) 104, 122, 748, 806, 1101
BceAI ACGGC 2 cut(s) 64, 301
BcgI CGANNNNNNTGC 4 cut(s) 335, 369, 574, 608
BciT130I CCWGG 1 cut(s) 676
BcoDI GTCTC 2 cut(s) 210, 507
BfaI CTAG 1 cut(s) 695
BfmI CTRYAG 1 cut(s) 768
BfoI RGCGCY 1 cut(s) 899
BglI GCCNNNNNGGC 2 cut(s) 316, 1121
BisI GCNGC 8 cut(s) 105, 289, 362, 447, 450, 560, 567, 1000
BlsI GCNGC 8 cut(s) 106, 290, 363, 448, 451, 561, 568, 1001
Bme1390I CCNGG 1 cut(s) 676
Bme18I GGWCC 1 cut(s) 1084
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 1084
BmiI GGNNCC 3 cut(s) 238, 940, 1085
BmrFI CCNGG 1 cut(s) 676
BmrI ACTGGG 3 cut(s) 324, 335, 378
BmsI GCATC 5 cut(s) 91, 707, 982, 1073, 1093
BmuI ACTGGG 3 cut(s) 324, 335, 378
BpmI CTGGAG 2 cut(s) 105, 697
BpuEI CTTGAG 1 cut(s) 72
Bsa29I ATCGAT 2 cut(s) 99, 688
BsaJI CCNNGG 5 cut(s) 250, 674, 702, 934, 942
BsaXI ACNNNNNCTCC 2 cut(s) 58, 88
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 770
Bse118I RCCGGY 1 cut(s) 163
Bse1I ACTGG 5 cut(s) 319, 341, 384, 406, 468
Bse3DI GCAATG 3 cut(s) 69, 334, 356
BseBI CCWGG 1 cut(s) 676
BseCI ATCGAT 2 cut(s) 99, 688
BseDI CCNNGG 5 cut(s) 250, 674, 702, 934, 942
BseGI GGATG 4 cut(s) 698, 817, 1093, 1186
BseLI CCNNNNNNNGG 2 cut(s) 163, 770
BseMI GCAATG 3 cut(s) 69, 334, 356
BseMII CTCAG 1 cut(s) 518
BseNI ACTGG 5 cut(s) 319, 341, 384, 406, 468
BseRI GAGGAG 5 cut(s) 126, 177, 211, 771, 842
BseX3I CGGCCG 1 cut(s) 286
BseXI GCAGC 5 cut(s) 433, 436, 553, 571, 986
BsgI GTGCAG 1 cut(s) 1130
Bsh1236I CGCG 1 cut(s) 291
Bsh1285I CGRYCG 2 cut(s) 274, 289
BshFI GGCC 4 cut(s) 167, 288, 396, 1143
BshNI GGYRCC 1 cut(s) 236
BshVI ATCGAT 2 cut(s) 99, 688
BsiEI CGRYCG 2 cut(s) 274, 289
BsiHKAI GWGCWC 2 cut(s) 597, 781
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 2 cut(s) 164, 234
BslFI GGGAC 3 cut(s) 179, 268, 1097
BslI CCNNNNNNNGG 2 cut(s) 163, 770
BsmAI GTCTC 2 cut(s) 210, 507
BsmFI GGGAC 3 cut(s) 179, 268, 1097
BsmI GAATGC 1 cut(s) 36
BsnI GGCC 4 cut(s) 167, 288, 396, 1143
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 3 cut(s) 312, 597, 781
Bsp143I GATC 2 cut(s) 9, 938
Bsp19I CCATGG 1 cut(s) 942
BspACI CCGC 7 cut(s) 51, 77, 105, 274, 289, 361, 1002
BspANI GGCC 4 cut(s) 167, 288, 396, 1143
BspCNI CTCAG 1 cut(s) 517
BspDI ATCGAT 2 cut(s) 99, 688
BspFNI CGCG 1 cut(s) 291
BspHI TCATGA 1 cut(s) 864
BspLI GGNNCC 3 cut(s) 238, 940, 1085
BspPI GGATC 2 cut(s) 933, 946
BspQI GCTCTTC 1 cut(s) 876
BspT107I GGYRCC 1 cut(s) 236
BsrDI GCAATG 3 cut(s) 69, 334, 356
BsrFI RCCGGY 1 cut(s) 163
BsrI ACTGG 5 cut(s) 319, 341, 384, 406, 468
BssAI RCCGGY 1 cut(s) 163
BssECI CCNNGG 5 cut(s) 250, 674, 702, 934, 942
BssMI GATC 2 cut(s) 9, 938
BssSI CACGAG 2 cut(s) 264, 305
BssT1I CCWWGG 2 cut(s) 934, 942
Bst2BI CACGAG 2 cut(s) 264, 305
Bst2UI CCWGG 1 cut(s) 676
Bst4CI ACNGT 2 cut(s) 706, 974
Bst6I CTCTTC 4 cut(s) 133, 209, 400, 876
BstAPI GCANNNNNTGC 1 cut(s) 1112
BstC8I GCNNGC 4 cut(s) 564, 997, 1054, 1145
BstDEI CTNAG 2 cut(s) 504, 887
BstDSI CCRYGG 2 cut(s) 702, 942
BstF5I GGATG 4 cut(s) 698, 817, 1093, 1186
BstFNI CGCG 1 cut(s) 291
BstH2I RGCGCY 1 cut(s) 899
BstHHI GCGC 1 cut(s) 898
BstKTI GATC 2 cut(s) 12, 941
BstMAI GTCTC 2 cut(s) 210, 507
BstMBI GATC 2 cut(s) 9, 938
BstMCI CGRYCG 2 cut(s) 274, 289
BstMWI GCNNNNNNNGC 7 cut(s) 316, 367, 446, 514, 1008, 1112, 1121
BstNI CCWGG 1 cut(s) 676
BstNSI RCATGY 1 cut(s) 494
BstSCI CCNGG 1 cut(s) 674
BstSFI CTRYAG 1 cut(s) 768
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 5 cut(s) 433, 436, 553, 571, 986
BstX2I RGATCY 1 cut(s) 938
BstYI RGATCY 1 cut(s) 938
BstZI CGGCCG 1 cut(s) 286
Bsu15I ATCGAT 2 cut(s) 99, 688
BsuRI GGCC 4 cut(s) 167, 288, 396, 1143
BsuTUI ATCGAT 2 cut(s) 99, 688
BtgI CCRYGG 2 cut(s) 702, 942
BtsCI GGATG 4 cut(s) 698, 817, 1093, 1186
BtsIMutI CAGTG 2 cut(s) 312, 475
Cac8I GCNNGC 4 cut(s) 564, 997, 1054, 1145
CciI TCATGA 1 cut(s) 864
CfoI GCGC 1 cut(s) 898
Cfr10I RCCGGY 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 1084
ClaI ATCGAT 2 cut(s) 99, 688
CseI GACGC 1 cut(s) 718
Csp6I GTAC 3 cut(s) 82, 380, 799
CviAII CATG 7 cut(s) 425, 491, 841, 865, 943, 1030, 1048
CviQI GTAC 3 cut(s) 82, 380, 799
DdeI CTNAG 2 cut(s) 504, 887
DpnI GATC 2 cut(s) 11, 940
DpnII GATC 2 cut(s) 9, 938
DrdI GACNNNNNNGTC 1 cut(s) 224
DseDI GACNNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 2 cut(s) 286, 394
EagI CGGCCG 1 cut(s) 286
Eam1104I CTCTTC 4 cut(s) 133, 209, 400, 876
EarI CTCTTC 4 cut(s) 133, 209, 400, 876
EciI GGCGGA 1 cut(s) 66
EclXI CGGCCG 1 cut(s) 286
Eco130I CCWWGG 2 cut(s) 934, 942
Eco24I GRGCYC 1 cut(s) 312
Eco47I GGWCC 1 cut(s) 1084
Eco47III AGCGCT 1 cut(s) 897
Eco52I CGGCCG 1 cut(s) 286
Eco88I CYCGRG 1 cut(s) 251
EcoRI GAATTC 1 cut(s) 586
EcoRII CCWGG 1 cut(s) 674
EcoT14I CCWWGG 2 cut(s) 934, 942
EcoT22I ATGCAT 1 cut(s) 1066
EcoT38I GRGCYC 1 cut(s) 312
ErhI CCWWGG 2 cut(s) 934, 942
FaeI CATG 7 cut(s) 428, 494, 844, 868, 946, 1033, 1051
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FaqI GGGAC 3 cut(s) 179, 268, 1097
FatI CATG 7 cut(s) 424, 490, 840, 864, 942, 1029, 1047
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 8 cut(s) 105, 289, 362, 447, 450, 560, 567, 1000
FokI GGATG 4 cut(s) 685, 824, 1080, 1193
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 8 cut(s) 105, 289, 362, 447, 450, 560, 567, 1000
FspBI CTAG 1 cut(s) 695
GlaI GCGC 1 cut(s) 897
GluI GCNGC 8 cut(s) 105, 289, 362, 447, 450, 560, 567, 1000
GsuI CTGGAG 2 cut(s) 105, 697
HaeII RGCGCY 1 cut(s) 899
HaeIII GGCC 4 cut(s) 167, 288, 396, 1143
HapII CCGG 2 cut(s) 164, 234
HgaI GACGC 1 cut(s) 718
HhaI GCGC 1 cut(s) 898
Hin1II CATG 7 cut(s) 428, 494, 844, 868, 946, 1033, 1051
Hin6I GCGC 1 cut(s) 896
HinP1I GCGC 1 cut(s) 896
HincII GTYRAC 2 cut(s) 229, 488
HindII GTYRAC 2 cut(s) 229, 488
HinfI GANTC 6 cut(s) 184, 218, 230, 336, 612, 647
HpaII CCGG 2 cut(s) 164, 234
HphI GGTGA 6 cut(s) 34, 457, 718, 736, 976, 1032
Hpy166II GTNNAC 3 cut(s) 229, 488, 522
Hpy188I TCNGA 5 cut(s) 215, 478, 611, 964, 1069
Hpy188III TCNNGA 6 cut(s) 89, 253, 583, 739, 865, 1135
Hpy8I GTNNAC 3 cut(s) 229, 488, 522
Hpy99I CGWCG 2 cut(s) 10, 230
HpyAV CCTTC 3 cut(s) 150, 736, 802
HpyCH4III ACNGT 2 cut(s) 706, 974
HpyCH4IV ACGT 2 cut(s) 654, 967
HpyCH4V TGCA 8 cut(s) 36, 327, 356, 658, 1056, 1064, 1106, 1147
HpyF10VI GCNNNNNNNGC 7 cut(s) 316, 367, 446, 514, 1008, 1112, 1121
HpyF3I CTNAG 2 cut(s) 504, 887
HpySE526I ACGT 2 cut(s) 654, 967
Hsp92II CATG 7 cut(s) 428, 494, 844, 868, 946, 1033, 1051
HspAI GCGC 1 cut(s) 896
Kzo9I GATC 2 cut(s) 9, 938
LguI GCTCTTC 1 cut(s) 876
LmnI GCTCC 2 cut(s) 79, 784
Lsp1109I GCAGC 5 cut(s) 433, 436, 553, 571, 986
LweI GCATC 5 cut(s) 91, 707, 982, 1073, 1093
MaeI CTAG 1 cut(s) 695
MaeII ACGT 2 cut(s) 654, 967
MaeIII GTNAC 3 cut(s) 706, 968, 982
MalI GATC 2 cut(s) 11, 940
MboI GATC 2 cut(s) 9, 938
MboII GAAGA 9 cut(s) 8, 11, 120, 123, 196, 417, 425, 893, 910
MflI RGATCY 1 cut(s) 938
MhlI GDGCHC 3 cut(s) 312, 597, 781
MlsI TGGCCA 1 cut(s) 396
MluCI AATT 7 cut(s) 244, 456, 479, 510, 586, 729, 1088
MluNI TGGCCA 1 cut(s) 396
MlyI GAGTC 4 cut(s) 178, 212, 224, 656
MmeI TCCRAC 1 cut(s) 1121
Mox20I TGGCCA 1 cut(s) 396
Mph1103I ATGCAT 1 cut(s) 1066
MroXI GAANNNNTTC 1 cut(s) 897
MscI TGGCCA 1 cut(s) 396
MseI TTAA 4 cut(s) 573, 732, 953, 1110
MslI CAYNNNNRTG 2 cut(s) 947, 1154
Msp20I TGGCCA 1 cut(s) 396
MspI CCGG 2 cut(s) 164, 234
MspR9I CCNGG 1 cut(s) 676
Mva1269I GAATGC 1 cut(s) 36
MvaI CCWGG 1 cut(s) 676
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 7 cut(s) 316, 367, 446, 514, 1008, 1112, 1121
NcoI CCATGG 1 cut(s) 942
NdeII GATC 2 cut(s) 9, 938
NlaIII CATG 7 cut(s) 428, 494, 844, 868, 946, 1033, 1051
NlaIV GGNNCC 3 cut(s) 238, 940, 1085
NmuCI GTSAC 2 cut(s) 706, 982
NsiI ATGCAT 1 cut(s) 1066
NspI RCATGY 1 cut(s) 494
PagI TCATGA 1 cut(s) 864
PciI ACATGT 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 876
PcsI WCGNNNNNNNCGW 2 cut(s) 267, 303
PctI GAATGC 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 897
PfeI GAWTC 2 cut(s) 336, 612
PkrI GCNGC 8 cut(s) 106, 290, 363, 448, 451, 561, 568, 1001
PleI GAGTC 4 cut(s) 178, 212, 224, 655
PpsI GAGTC 4 cut(s) 178, 212, 224, 655
PscI ACATGT 1 cut(s) 490
PshBI ATTAAT 2 cut(s) 573, 732
Psp1406I AACGTT 1 cut(s) 967
Psp6I CCWGG 1 cut(s) 674
PspGI CCWGG 1 cut(s) 674
PspN4I GGNNCC 3 cut(s) 238, 940, 1085
PspPI GGNCC 1 cut(s) 1084
PsuI RGATCY 1 cut(s) 938
RsaI GTAC 3 cut(s) 83, 381, 800
RsaNI GTAC 3 cut(s) 82, 380, 799
RseI CAYNNNNRTG 2 cut(s) 947, 1154
SalI GTCGAC 1 cut(s) 227
SapI GCTCTTC 1 cut(s) 876
SaqAI TTAA 4 cut(s) 573, 732, 953, 1110
SatI GCNGC 8 cut(s) 105, 289, 362, 447, 450, 560, 567, 1000
Sau3AI GATC 2 cut(s) 9, 938
Sau96I GGNCC 1 cut(s) 1084
SchI GAGTC 4 cut(s) 178, 212, 224, 656
ScrFI CCNGG 1 cut(s) 676
SduI GDGCHC 3 cut(s) 312, 597, 781
SetI ASST 9 cut(s) 568, 657, 726, 747, 769, 775, 872, 888, 970
SfaNI GCATC 5 cut(s) 91, 707, 982, 1073, 1093
SfcI CTRYAG 1 cut(s) 768
SinI GGWCC 1 cut(s) 1084
SmiMI CAYNNNNRTG 2 cut(s) 947, 1154
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
Sse9I AATT 7 cut(s) 244, 456, 479, 510, 586, 729, 1088
SsiI CCGC 7 cut(s) 51, 77, 105, 274, 289, 361, 1002
SspMI CTAG 1 cut(s) 695
StyD4I CCNGG 1 cut(s) 674
StyI CCWWGG 2 cut(s) 934, 942
TaaI ACNGT 2 cut(s) 706, 974
TaiI ACGT 2 cut(s) 657, 970
TaqI TCGA 7 cut(s) 99, 228, 297, 345, 584, 650, 688
TasI AATT 7 cut(s) 244, 456, 479, 510, 586, 729, 1088
TatI WGTACW 1 cut(s) 798
TauI GCSGC 3 cut(s) 107, 291, 364
TfiI GAWTC 2 cut(s) 336, 612
Tru1I TTAA 4 cut(s) 573, 732, 953, 1110
Tru9I TTAA 4 cut(s) 573, 732, 953, 1110
TscAI CASTG 2 cut(s) 319, 475
TseFI GTSAC 2 cut(s) 706, 982
TseI GCWGC 5 cut(s) 446, 449, 559, 566, 999
Tsp45I GTSAC 2 cut(s) 706, 982
TspDTI ATGAA 2 cut(s) 413, 1184
TspGWI ACGGA 2 cut(s) 214, 891
TspRI CASTG 2 cut(s) 319, 475
VpaK11BI GGWCC 1 cut(s) 1084
VspI ATTAAT 2 cut(s) 573, 732
XapI RAATTY 2 cut(s) 510, 586
XceI RCATGY 1 cut(s) 494
XmiI GTMKAC 1 cut(s) 228
XmnI GAANNNNTTC 1 cut(s) 897
XspI CTAG 1 cut(s) 695
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.