AT4G12700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012448
Physical Location & Seq
Reverse (-)
7482361 .. 7484566
2206 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12700.1

Sequence Viewer

Length: 1686 bp
ATGTCAGGAAGATCTGCATTTTCCAGAACCGGAGGTTTCCGACCTGAGAATCTAGGGCAAAACGCAGTATCCTTAATCGGAAGCATAGGCTTCTCTGTACTCGTAATCGGAGTCGTAGTTTTCACCATTATCGCTGCTACATATGAACCAGAAGATCCACTATTTCACCCATCTGACAAAATCACAACTTTCCTCACATCCAATTCAAACGCTACTCTTAAATCCGACGACAGTATTGTCAAAACCGGTGAAGATTTCATGGCGGCGAACCAAACCGCGTTTGGCGGATTCATCAATATAGCTGATGTTGAAACATCTGAGAATGACTCTGATGGTAATCAATTGGATTGTGATACCAATATCCCAATTGATTGCAAAGATCCTGAAGTTTTTCACTTGATGATGAAAGCTACTATGGAGAAGTTTAAAGACAGTCACTTTTATAAGTTTGGTAAACCTGTGATTGTGGAAGGTAGTAGTAGTTCTTGTGATATGGCTTGGCGTTATAGACCTAAGGATGGTAAAGCGGCTGCGTTTTATAAGGATTATAGGAGGTTTGTGATTGAAAAGTCTGGGAATTGTAGTGTTAGTGTGATGGGGATTGGTGAGTATCATTCGGGTGTGAATGCGAGGAAGCGGAAGAGGCCCGGGTTTCGAAATTCGAGTGGTGGTAAGGTTGATGATTTCGCATTGCCTGTAGTTGGTGAAGCAGTGAATGATTCACTTCCTGTTGTTGAGTCTGAAAATGTGTTTAAAGAAGGTCATTACTTGGTTTATTCTGGTGGAGGAGATAGGTGTAAGAGTATGAACCATTTCTTGTGGAGTTTCTTGTGTGCTTTAGGGGAAGCTCAGTATTTGAATAGAACATTAGTGATGGATTTGACTCTTTGTTTGTCTTCTGTTTATACATTGTCTGGTCAAAACGAGGAAGGGAAGGACTTTAGGTTTTACTTTGATTTCGAGCATTTGAAAGAGGCTGCTTCTATGTTAGACCAGGTGCAGTTTTGGGCTGATTGGGGGAAATGGTATAAGAAGAATGGATTAAAGCTTCATCTTGTGGAAGATTTTCGGGTCACACCGATGAAGCTTGTTGATGTGAAGGACACGTTGATAATGAGGAAGTTCGGGACAGTAGAACCAGATAACTATTGGTATAGAGTATGCGAAGGGGAGACAGAATCTGTTGTGCAAAGGCCTTGGAATCTATTGTGGAAATCTAAACGACTGATGGAGATTGTTTCTGCGATTGCTTCGAGGTTAAACTGGGATTATGATGCTATTCACATTGAGAGAGGAGACAAGGCGAGAAACAAGGAAGTTTGGCCTAATCTTGAAAAGGATACTTCACCAAGCTCCATTTTATCTACCCTCCAGGACAAAATCGAACAAGGAAGGAATCTTTATATTGCAACAAATGAACCAGAGTTATCTTTCTTTAACCCCTTGAAAGACAAGTACAAACCCCATTTTCTGGATGAGTTTAAGGATCTCTGGGACGAGAGCAGCGAATGGTATTCAGAGACAACGAAGCTTAATGGAGGAAACCCAGTTGAGTTTGACGGTTACATGAGAGCGTCTGTTGATACAGAGGTGTTCTTGAGAGGGAAGAAGCAGATTGAAACATTCAATGATCTTACCAATGACTGTAGAGATGGAATCGGCACTTGCAACGTAGCAGCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

561

Amino Acids

63.49

Weight (kDa)

5.27

Isoelectric Point (pI)

33.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7074 PF23269 122 - 206 4.3e-36 Domain of unknown function (DUF7074)
DUF7075 PF23272 251 - 541 2.8e-87 Domain of unknown function (DUF7075)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012448)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04280 AT4G12700
fragaria_vesca FvH4_6g40420
malus_domestica MD09G1130100.v1.1 MD17G1105300.v1.1
prunus_persica Prupe.3G195000_v2.0.a1 Prupe.3G195000_v2.0.a1
pyrus_communis pycom09g05320
rosa_chinensis RchiOBHm_Chr2g0155651
rosa_laevigata RLG00000020851
rosa_multiflora Rmu_ssc0000430.1_g000005
rosa_roxburghii Rroxscaffold_1G00014560 Rroxscaffold_2G00093590
rosa_rugosa Rorug02G0448800
rosa_samantha Rh2AG514900 Rh2BG525800 Rh2CG499800 Rh2DG534700
rosa_wichuraiana Rw2G042310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 444, 540
AasI GACNNNNNNGTC 1 cut(s) 236
AccB7I CCANNNNNTGG 1 cut(s) 1471
AccII CGCG 1 cut(s) 278
AciI CCGC 5 cut(s) 263, 276, 285, 527, 637
AclWI GGATC 3 cut(s) 149, 374, 1494
AcsI RAATTY 1 cut(s) 658
AcuI CTGAAG 1 cut(s) 405
AfaI GTAC 2 cut(s) 99, 1457
AfiI CCNNNNNNNGG 3 cut(s) 518, 701, 1471
AflIII ACRYGT 1 cut(s) 1104
AgeI ACCGGT 1 cut(s) 245
AgsI TTSAA 9 cut(s) 207, 311, 566, 859, 970, 1334, 1447, 1619, 1627
AjnI CCWGG 2 cut(s) 993, 1371
AluBI AGCT 8 cut(s) 302, 410, 848, 1048, 1087, 1353, 1531, 1683
AluI AGCT 8 cut(s) 302, 410, 848, 1048, 1087, 1353, 1531, 1683
Alw26I GTCTC 3 cut(s) 1166, 1290, 1514
AlwI GGATC 3 cut(s) 149, 374, 1494
AlwNI CAGNNNCTG 1 cut(s) 1181
Ama87I CYCGRG 1 cut(s) 647
AoxI GGCC 3 cut(s) 644, 1193, 1322
ApeKI GCWGC 5 cut(s) 134, 530, 977, 1503, 1676
ApoI RAATTY 1 cut(s) 658
ArsI GACNNNNNNTTYG 2 cut(s) 874, 906
AsiGI ACCGGT 1 cut(s) 245
Asp700I GAANNNNTTC 3 cut(s) 390, 812, 1065
AspS9I GGNCC 1 cut(s) 645
AsuC2I CCSGG 2 cut(s) 648, 649
AsuHPI GGTGA 6 cut(s) 115, 158, 260, 617, 716, 1338
AsuII TTCGAA 1 cut(s) 655
AvaI CYCGRG 1 cut(s) 647
AxyI CCTNAGG 1 cut(s) 513
BaeI ACNNNNGTAYC 2 cut(s) 1575, 1608
BbsI GAAGAC 1 cut(s) 888
BbvI GCAGC 4 cut(s) 121, 517, 964, 1515
BccI CCATC 7 cut(s) 178, 326, 512, 589, 868, 1222, 1646
BciT130I CCWGG 2 cut(s) 995, 1373
BciVI GTATCC 2 cut(s) 79, 1333
BcnI CCSGG 2 cut(s) 648, 649
BcoDI GTCTC 3 cut(s) 1166, 1290, 1514
BfaI CTAG 1 cut(s) 53
BfmI CTRYAG 2 cut(s) 696, 1645
BfuI GTATCC 2 cut(s) 79, 1333
BglII AGATCT 1 cut(s) 11
BisI GCNGC 7 cut(s) 135, 264, 528, 531, 978, 1504, 1677
BlsI GCNGC 7 cut(s) 136, 265, 529, 532, 979, 1505, 1678
Bme1390I CCNGG 4 cut(s) 648, 649, 995, 1373
BmeT110I CYCGRG 1 cut(s) 647
BmgT120I GGNCC 1 cut(s) 645
BmrFI CCNGG 4 cut(s) 648, 649, 995, 1373
BmrI ACTGGG 2 cut(s) 1273, 1541
BmsI GCATC 1 cut(s) 1264
BmuI ACTGGG 2 cut(s) 1273, 1541
BpiI GAAGAC 1 cut(s) 888
BplI GAGNNNNNCTC 2 cut(s) 311, 343
BpmI CTGGAG 1 cut(s) 1355
Bpu14I TTCGAA 1 cut(s) 655
BpuEI CTTGAG 1 cut(s) 1618
BpuMI CCSGG 2 cut(s) 648, 649
BsaBI GATNNNNATC 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 647, 1196
BsaWI WCCGGW 2 cut(s) 29, 245
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 3 cut(s) 518, 701, 1471
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 2 cut(s) 1268, 1547
Bse21I CCTNAGG 1 cut(s) 513
Bse3DI GCAATG 1 cut(s) 689
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 2 cut(s) 995, 1373
BseDI CCNNGG 2 cut(s) 647, 1196
BseGI GGATG 3 cut(s) 197, 523, 1480
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 3 cut(s) 518, 701, 1471
BseMI GCAATG 1 cut(s) 689
BseMII CTCAG 3 cut(s) 36, 309, 863
BseNI ACTGG 2 cut(s) 1268, 1547
BseRI GAGGAG 2 cut(s) 801, 1308
BseXI GCAGC 4 cut(s) 121, 517, 964, 1515
BsgI GTGCAG 1 cut(s) 1019
Bsh1236I CGCG 1 cut(s) 278
BshFI GGCC 3 cut(s) 646, 1195, 1324
BshTI ACCGGT 1 cut(s) 245
BsiHKCI CYCGRG 1 cut(s) 647
BsiSI CCGG 3 cut(s) 30, 246, 648
BslFI GGGAC 2 cut(s) 1141, 1508
BslI CCNNNNNNNGG 3 cut(s) 518, 701, 1471
BsmAI GTCTC 3 cut(s) 1166, 1290, 1514
BsmFI GGGAC 2 cut(s) 1141, 1508
BsmI GAATGC 1 cut(s) 631
BsnI GGCC 3 cut(s) 646, 1195, 1324
BsoBI CYCGRG 1 cut(s) 647
Bsp119I TTCGAA 1 cut(s) 655
Bsp143I GATC 5 cut(s) 11, 154, 379, 1486, 1630
BspACI CCGC 5 cut(s) 263, 276, 285, 527, 637
BspANI GGCC 3 cut(s) 646, 1195, 1324
BspCNI CTCAG 3 cut(s) 37, 310, 862
BspFNI CGCG 1 cut(s) 278
BspPI GGATC 3 cut(s) 149, 374, 1494
BspT104I TTCGAA 1 cut(s) 655
BsrDI GCAATG 1 cut(s) 689
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 2 cut(s) 1268, 1547
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 2 cut(s) 647, 1196
BssMI GATC 5 cut(s) 11, 154, 379, 1486, 1630
BssT1I CCWWGG 1 cut(s) 1196
Bst2UI CCWGG 2 cut(s) 995, 1373
Bst4CI ACNGT 5 cut(s) 233, 434, 1132, 1562, 1646
Bst6I CTCTTC 1 cut(s) 635
BstBI TTCGAA 1 cut(s) 655
BstC8I GCNNGC 1 cut(s) 1681
BstDEI CTNAG 4 cut(s) 45, 318, 513, 849
BstF5I GGATG 3 cut(s) 197, 523, 1480
BstFNI CGCG 1 cut(s) 278
BstKTI GATC 5 cut(s) 14, 157, 382, 1489, 1633
BstMAI GTCTC 3 cut(s) 1166, 1290, 1514
BstMBI GATC 5 cut(s) 11, 154, 379, 1486, 1630
BstMWI GCNNNNNNNGC 1 cut(s) 643
BstNI CCWGG 2 cut(s) 995, 1373
BstSCI CCNGG 4 cut(s) 646, 647, 993, 1371
BstSFI CTRYAG 2 cut(s) 696, 1645
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 4 cut(s) 121, 517, 964, 1515
BstV2I GAAGAC 1 cut(s) 888
BstX2I RGATCY 4 cut(s) 11, 154, 379, 1486
BstYI RGATCY 4 cut(s) 11, 154, 379, 1486
Bsu36I CCTNAGG 1 cut(s) 513
BsuI GTATCC 2 cut(s) 79, 1333
BsuRI GGCC 3 cut(s) 646, 1195, 1324
BtsCI GGATG 3 cut(s) 197, 523, 1480
BtsI GCAGTG 1 cut(s) 717
BtsIMutI CAGTG 1 cut(s) 717
Cac8I GCNNGC 1 cut(s) 1681
CaiI CAGNNNCTG 1 cut(s) 1181
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 1 cut(s) 645
Cfr9I CCCGGG 1 cut(s) 647
CseI GACGC 1 cut(s) 1563
CsiI ACCWGGT 1 cut(s) 993
Csp6I GTAC 2 cut(s) 98, 1456
CspAI ACCGGT 1 cut(s) 245
CviAII CATG 2 cut(s) 259, 1567
CviQI GTAC 2 cut(s) 98, 1456
DdeI CTNAG 4 cut(s) 45, 318, 513, 849
DpnI GATC 5 cut(s) 13, 156, 381, 1488, 1632
DpnII GATC 5 cut(s) 11, 154, 379, 1486, 1630
DraI TTTAAA 2 cut(s) 427, 754
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
Eam1104I CTCTTC 1 cut(s) 635
EarI CTCTTC 1 cut(s) 635
EciI GGCGGA 1 cut(s) 300
Eco130I CCWWGG 1 cut(s) 1196
Eco147I AGGCCT 1 cut(s) 1195
Eco57I CTGAAG 1 cut(s) 405
Eco81I CCTNAGG 1 cut(s) 513
Eco88I CYCGRG 1 cut(s) 647
EcoRII CCWGG 2 cut(s) 993, 1371
EcoT14I CCWWGG 1 cut(s) 1196
ErhI CCWWGG 1 cut(s) 1196
FaeI CATG 2 cut(s) 262, 1570
FaqI GGGAC 2 cut(s) 1141, 1508
FatI CATG 2 cut(s) 258, 1566
FauNDI CATATG 1 cut(s) 142
Fnu4HI GCNGC 7 cut(s) 135, 264, 528, 531, 978, 1504, 1677
FokI GGATG 3 cut(s) 184, 530, 1487
Fsp4HI GCNGC 7 cut(s) 135, 264, 528, 531, 978, 1504, 1677
FspBI CTAG 1 cut(s) 53
GluI GCNGC 7 cut(s) 135, 264, 528, 531, 978, 1504, 1677
GsuI CTGGAG 1 cut(s) 1355
HaeIII GGCC 3 cut(s) 646, 1195, 1324
HapII CCGG 3 cut(s) 30, 246, 648
HgaI GACGC 1 cut(s) 1563
Hin1II CATG 2 cut(s) 262, 1570
HindIII AAGCTT 3 cut(s) 1046, 1085, 1529
HpaII CCGG 3 cut(s) 30, 246, 648
HphI GGTGA 6 cut(s) 115, 158, 260, 617, 716, 1338
Hpy166II GTNNAC 1 cut(s) 455
Hpy188I TCNGA 9 cut(s) 41, 80, 110, 175, 226, 319, 331, 742, 1519
Hpy188III TCNNGA 7 cut(s) 6, 24, 383, 1126, 1331, 1472, 1597
Hpy8I GTNNAC 1 cut(s) 455
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 7 cut(s) 464, 752, 923, 928, 1093, 1160, 1386
HpyCH4III ACNGT 5 cut(s) 233, 434, 1132, 1562, 1646
HpyCH4IV ACGT 2 cut(s) 1106, 1671
HpyCH4V TGCA 6 cut(s) 17, 375, 1000, 1189, 1409, 1668
HpyF10VI GCNNNNNNNGC 1 cut(s) 643
HpyF3I CTNAG 4 cut(s) 45, 318, 513, 849
HpySE526I ACGT 2 cut(s) 1106, 1671
Hsp92II CATG 2 cut(s) 262, 1570
Kzo9I GATC 5 cut(s) 11, 154, 379, 1486, 1630
LmnI GCTCC 1 cut(s) 1358
Lsp1109I GCAGC 4 cut(s) 121, 517, 964, 1515
LweI GCATC 1 cut(s) 1264
MabI ACCWGGT 1 cut(s) 993
MaeI CTAG 1 cut(s) 53
MaeII ACGT 2 cut(s) 1106, 1671
MaeIII GTNAC 3 cut(s) 434, 1072, 1562
MalI GATC 5 cut(s) 13, 156, 381, 1488, 1632
MboI GATC 5 cut(s) 11, 154, 379, 1486, 1630
MboII GAAGA 8 cut(s) 21, 164, 263, 652, 888, 1045, 1073, 1618
MfeI CAATTG 2 cut(s) 341, 366
MflI RGATCY 4 cut(s) 11, 154, 379, 1486
MluCI AATT 5 cut(s) 202, 341, 366, 577, 658
MlyI GAGTC 4 cut(s) 120, 320, 746, 877
MmeI TCCRAC 2 cut(s) 64, 249
MroXI GAANNNNTTC 3 cut(s) 390, 812, 1065
MseI TTAA 9 cut(s) 74, 219, 426, 753, 1043, 1259, 1437, 1482, 1533
MslI CAYNNNNRTG 2 cut(s) 618, 1079
MspI CCGG 3 cut(s) 30, 246, 648
MspR9I CCNGG 4 cut(s) 648, 649, 995, 1373
MunI CAATTG 2 cut(s) 341, 366
Mva1269I GAATGC 1 cut(s) 631
MvaI CCWGG 2 cut(s) 995, 1373
MvnI CGCG 1 cut(s) 278
MwoI GCNNNNNNNGC 1 cut(s) 643
NciI CCSGG 2 cut(s) 648, 649
NdeI CATATG 1 cut(s) 142
NdeII GATC 5 cut(s) 11, 154, 379, 1486, 1630
NlaIII CATG 2 cut(s) 262, 1570
NmuCI GTSAC 2 cut(s) 434, 1072
NspV TTCGAA 1 cut(s) 655
PceI AGGCCT 1 cut(s) 1195
PcsI WCGNNNNNNNCGW 1 cut(s) 1503
PctI GAATGC 1 cut(s) 631
PdmI GAANNNNTTC 3 cut(s) 390, 812, 1065
PfeI GAWTC 7 cut(s) 49, 288, 719, 1178, 1201, 1396, 1656
PflMI CCANNNNNTGG 1 cut(s) 1471
PfoI TCCNGGA 1 cut(s) 1371
PinAI ACCGGT 1 cut(s) 245
PkrI GCNGC 7 cut(s) 136, 265, 529, 532, 979, 1505, 1678
PleI GAGTC 4 cut(s) 119, 320, 745, 877
PpsI GAGTC 4 cut(s) 119, 320, 745, 877
PsiI TTATAA 2 cut(s) 444, 540
Psp6I CCWGG 2 cut(s) 993, 1371
PspGI CCWGG 2 cut(s) 993, 1371
PspPI GGNCC 1 cut(s) 645
PsrI GAACNNNNNNTAC 2 cut(s) 466, 498
PstNI CAGNNNCTG 1 cut(s) 1181
PsuI RGATCY 4 cut(s) 11, 154, 379, 1486
RsaI GTAC 2 cut(s) 99, 1457
RsaNI GTAC 2 cut(s) 98, 1456
RseI CAYNNNNRTG 2 cut(s) 618, 1079
SaqAI TTAA 9 cut(s) 74, 219, 426, 753, 1043, 1259, 1437, 1482, 1533
SatI GCNGC 7 cut(s) 135, 264, 528, 531, 978, 1504, 1677
Sau3AI GATC 5 cut(s) 11, 154, 379, 1486, 1630
Sau96I GGNCC 1 cut(s) 645
SchI GAGTC 4 cut(s) 120, 320, 746, 877
ScrFI CCNGG 4 cut(s) 648, 649, 995, 1373
SexAI ACCWGGT 1 cut(s) 993
SfaNI GCATC 1 cut(s) 1264
SfcI CTRYAG 2 cut(s) 696, 1645
SfuI TTCGAA 1 cut(s) 655
SmaI CCCGGG 1 cut(s) 649
SmiMI CAYNNNNRTG 2 cut(s) 618, 1079
SmlI CTYRAG 1 cut(s) 1597
SmoI CTYRAG 1 cut(s) 1597
Sse9I AATT 5 cut(s) 202, 341, 366, 577, 658
SseBI AGGCCT 1 cut(s) 1195
SsiI CCGC 5 cut(s) 263, 276, 285, 527, 637
SspMI CTAG 1 cut(s) 53
StuI AGGCCT 1 cut(s) 1195
StyD4I CCNGG 4 cut(s) 646, 647, 993, 1371
StyI CCWWGG 1 cut(s) 1196
TaaI ACNGT 5 cut(s) 233, 434, 1132, 1562, 1646
TaiI ACGT 2 cut(s) 1109, 1674
TaqI TCGA 5 cut(s) 655, 662, 960, 1253, 1383
TasI AATT 5 cut(s) 202, 341, 366, 577, 658
TatI WGTACW 2 cut(s) 97, 1455
TauI GCSGC 2 cut(s) 266, 530
TfiI GAWTC 7 cut(s) 49, 288, 719, 1178, 1201, 1396, 1656
Tru1I TTAA 9 cut(s) 74, 219, 426, 753, 1043, 1259, 1437, 1482, 1533
Tru9I TTAA 9 cut(s) 74, 219, 426, 753, 1043, 1259, 1437, 1482, 1533
TscAI CASTG 1 cut(s) 717
TseFI GTSAC 2 cut(s) 434, 1072
TseI GCWGC 5 cut(s) 134, 530, 977, 1503, 1676
Tsp45I GTSAC 2 cut(s) 434, 1072
TspDTI ATGAA 8 cut(s) 159, 247, 280, 419, 821, 1040, 1097, 1431
TspMI CCCGGG 1 cut(s) 647
TspRI CASTG 1 cut(s) 717
Van91I CCANNNNNTGG 1 cut(s) 1471
XapI RAATTY 1 cut(s) 658
XcmI CCANNNNNNNNNTGG 2 cut(s) 278, 1146
XmaI CCCGGG 1 cut(s) 647
XmnI GAANNNNTTC 3 cut(s) 390, 812, 1065
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.