Prupe.3G195000_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0012448
Physical Location & Seq
Reverse (-)
20655725 .. 20658253
2529 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G195000.1

Sequence Viewer

Length: 1704 bp
ATGTTGGGTCGGTCCTCATTTTCCAGAACTGGAAGCTTTAGGCCTGAGAATTTGGGCCCAAATGCCCTTGCCTTGATTGGAAACCTTTGCTTCACGTTTTTTGTGCTCGGAGTGTTGATCTTCACCATTATTGCCGCCACCTATGAACCTGAAGACCCTTTGTTTCACCCATCAACCAAGATCACCACTTTCCTCACATCCAAATCCAATGCCACTTTCAAATCCGATGACACTGTGGTCAGGACTGGTGAGAATTTCATGGCTCCCAATCAAACTGAATTTGCAACCTTTATCAATATGACTGATGTTGTTAACTTAAGCGAGGGTGATAACACGATCAGTGACGCTTCTGACGCCGTCGCATCTTCTAGCTGTGAGGGTCCCATAGATTGCCGGGACCCGGAAGTGTTCCATTTGTTGATGAGGGCCACCATTGAAAAGTTTAAGGATATCCATTTTTACCGGTTTGGGAAACCTGTGCGGGGTTCGGATGATAACAGTTGTGATATGGCTTGGCGGTTTAGGCCTAAGGAAGGGAAGACTGCTTCCTTTTACAAGGATTACAGGAGGTTTACTATTTTGAAGTCTGAAAATTGTACTCTTAGTGTTGGTGACATTGGAGAGTACCATACTGGAGTAAATGCCAGGAAGAGGAAGAAGAATCAGAAGCCTGGGTTTGAGAAGAATGGGAAGAAGGAGGTGAATTCTTTGCCAGTTGTGGGGGAGATTGTGAATGATACCCTTCCGGTGGTTGAGTCTGAAGGTTCATTTAGTCGTGGGAAGTACTTAGTTTACATGGGTGGTGGAGATAGGTGCAAGAGCATGAACCATTACTTGTGGAGTTTCTTGTGTGCTTTAGGTGAAGCACAATACCTGAACCGAACTTTGGTCATGGAATTGAATATTTGTCTGTCGTCCATGTACACTTCGTCGAATCAAGATGAGGAAGGGAAGGATTTCAGGTTTTACTTTGACTTTGAGCATTTAAGAGAGTCTGCATCTGTATTAGACGCAAAGCAGTTCTGGTCAGATTGGGAGAAGTGGCAGAAGAAAGATGGGTTGAATCTTCATCTTGTGGAGGATTTTAGGGTCACACCTATGAAACTTAATGACGTCAAGGATGCTTTGATTATGAGAAAGTTCGGGTCCGTGGAGCCAGACAATTATTGGTATAGGGTGTGTGAAGGGGAAACGGAGTCTGTTGTTCAACGACCATGGCATTTGATATGGAAATCGAGAAGGTTAATGGATATAGTGTCTGCAATCTCATCAAAATTGAACTGGGATTATGATTCTGTGCATATTGAAAGAGGAGAGAAGGCACATAACAAGGAGCTTTGGCCTAACCTTGATGCAGATACTTCACCTAATGCACTTCTCTCAACCCTGCAGGACAAAATTGAAGATGGAAGGAACCTTTACATTGCAACAAACGAACCAGATACATCTTTCTTTGACCCTTTGAAGGACAAGTATACCACTCATTTCCTTGACGAGTACAAGGATCTTTGGGATGAAAACAGTGAGTGGTACTTCGACACCACAAAGCTTAACAATGGAGTTCCAGTTGAATTTGATGGTTACATGCGAGTGTCAGTTGATACAGAGGTGTTCTTGAGAGGGAAAAAGCAGATAGAAACATTCAATGATCTCACCAGTGATTGCAAGGATGGCATCAACACCTGCAGCACTGCGACCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

568

Amino Acids

64.93

Weight (kDa)

5.22

Isoelectric Point (pI)

35.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012448)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04280 AT4G12700
fragaria_vesca FvH4_6g40420
malus_domestica MD09G1130100.v1.1 MD17G1105300.v1.1
prunus_persica Prupe.3G195000_v2.0.a1 Prupe.3G195000_v2.0.a1
pyrus_communis pycom09g05320
rosa_chinensis RchiOBHm_Chr2g0155651
rosa_laevigata RLG00000020851
rosa_multiflora Rmu_ssc0000430.1_g000005
rosa_roxburghii Rroxscaffold_1G00014560 Rroxscaffold_2G00093590
rosa_rugosa Rorug02G0448800
rosa_samantha Rh2AG514900 Rh2BG525800 Rh2CG499800 Rh2DG534700
rosa_wichuraiana Rw2G042310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1691
AasI GACNNNNNNGTC 1 cut(s) 236
AatII GACGTC 1 cut(s) 1116
Acc36I ACCTGC 1 cut(s) 1691
AccI GTMKAC 1 cut(s) 1475
AciI CCGC 3 cut(s) 135, 481, 517
AclWI GGATC 1 cut(s) 1512
AcsI RAATTY 5 cut(s) 49, 253, 278, 703, 1571
AcuI CTGAAG 2 cut(s) 171, 780
AcyI GRCGYC 2 cut(s) 354, 1113
AfaI GTAC 6 cut(s) 598, 626, 785, 923, 1499, 1532
AfiI CCNNNNNNNGG 8 cut(s) 400, 482, 533, 651, 719, 748, 886, 1465
AflII CTTAAG 1 cut(s) 316
AgeI ACCGGT 1 cut(s) 462
AjnI CCWGG 2 cut(s) 644, 670
AjuI GAANNNNNNNTTGG 4 cut(s) 200, 232, 869, 901
AluBI AGCT 4 cut(s) 36, 372, 1336, 1549
AluI AGCT 4 cut(s) 36, 372, 1336, 1549
Alw21I GWGCWC 1 cut(s) 108
AlwI GGATC 1 cut(s) 1512
AoxI GGCC 5 cut(s) 41, 55, 426, 524, 1340
ApaI GGGCCC 1 cut(s) 59
ApeKI GCWGC 1 cut(s) 1686
ApoI RAATTY 5 cut(s) 49, 253, 278, 703, 1571
AsiGI ACCGGT 1 cut(s) 462
Asp700I GAANNNNTTC 1 cut(s) 956
AspS9I GGNCC 7 cut(s) 12, 55, 56, 380, 397, 426, 1146
AsuC2I CCSGG 2 cut(s) 395, 401
AvaII GGWCC 4 cut(s) 12, 380, 397, 1146
AxyI CCTNAGG 1 cut(s) 528
BaeGI GKGCMC 1 cut(s) 59
BaeI ACNNNNGTAYC 2 cut(s) 1593, 1626
BanII GRGCYC 1 cut(s) 59
BbsI GAAGAC 2 cut(s) 159, 545
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 1 cut(s) 1698
BccI CCATC 5 cut(s) 178, 1049, 1400, 1571, 1664
BceAI ACGGC 1 cut(s) 341
BciT130I CCWGG 2 cut(s) 646, 672
BcnI CCSGG 2 cut(s) 395, 401
BfaI CTAG 1 cut(s) 369
BfmI CTRYAG 2 cut(s) 1388, 1684
BfrI CTTAAG 1 cut(s) 316
BfuAI ACCTGC 1 cut(s) 1691
BisI GCNGC 2 cut(s) 135, 1687
BlsI GCNGC 2 cut(s) 136, 1688
BmcAI AGTACT 1 cut(s) 785
Bme1390I CCNGG 4 cut(s) 395, 401, 646, 672
Bme18I GGWCC 4 cut(s) 12, 380, 397, 1146
BmgT120I GGNCC 7 cut(s) 12, 55, 56, 380, 397, 426, 1146
BmiI GGNNCC 9 cut(s) 57, 264, 381, 382, 398, 399, 1147, 1155, 1415
BmrFI CCNGG 4 cut(s) 395, 401, 646, 672
BmrI ACTGGG 1 cut(s) 1291
BmsI GCATC 5 cut(s) 371, 1007, 1111, 1342, 1683
BmuI ACTGGG 1 cut(s) 1291
BpiI GAAGAC 2 cut(s) 159, 545
BpmI CTGGAG 1 cut(s) 654
BpuEI CTTGAG 1 cut(s) 1636
BpuMI CCSGG 2 cut(s) 395, 401
BsaHI GRCGYC 2 cut(s) 354, 1113
BsaJI CCNNGG 3 cut(s) 671, 1149, 1214
BsaWI WCCGGW 2 cut(s) 462, 745
BsaXI ACNNNNNCTCC 2 cut(s) 1551, 1581
Bsc4I CCNNNNNNNGG 8 cut(s) 400, 482, 533, 651, 719, 748, 886, 1465
Bse118I RCCGGY 1 cut(s) 462
Bse1I ACTGG 8 cut(s) 34, 250, 637, 713, 1286, 1565, 1656, 1698
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 1 cut(s) 1422
BseBI CCWGG 2 cut(s) 646, 672
BseDI CCNNGG 3 cut(s) 671, 1149, 1214
BseGI GGATG 5 cut(s) 197, 496, 1126, 1519, 1675
BseLI CCNNNNNNNGG 8 cut(s) 400, 482, 533, 651, 719, 748, 886, 1465
BseMI GCAATG 1 cut(s) 1422
BseMII CTCAG 1 cut(s) 36
BseNI ACTGG 8 cut(s) 34, 250, 637, 713, 1286, 1565, 1656, 1698
BseRI GAGGAG 1 cut(s) 1326
BseSI GKGCMC 1 cut(s) 59
BseXI GCAGC 1 cut(s) 1698
BshFI GGCC 5 cut(s) 43, 57, 428, 526, 1342
BshTI ACCGGT 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 108
BsiSI CCGG 4 cut(s) 394, 401, 463, 746
BslFI GGGAC 2 cut(s) 366, 410
BslI CCNNNNNNNGG 8 cut(s) 400, 482, 533, 651, 719, 748, 886, 1465
BsmFI GGGAC 2 cut(s) 366, 410
BsnI GGCC 5 cut(s) 43, 57, 428, 526, 1342
Bsp120I GGGCCC 1 cut(s) 55
Bsp1286I GDGCHC 2 cut(s) 59, 108
Bsp1407I TGTACA 1 cut(s) 921
Bsp143I GATC 5 cut(s) 117, 180, 336, 1504, 1648
Bsp19I CCATGG 1 cut(s) 1214
BspACI CCGC 3 cut(s) 135, 481, 517
BspANI GGCC 5 cut(s) 43, 57, 428, 526, 1342
BspCNI CTCAG 1 cut(s) 37
BspLI GGNNCC 9 cut(s) 57, 264, 381, 382, 398, 399, 1147, 1155, 1415
BspMAI CTGCAG 2 cut(s) 1392, 1688
BspMI ACCTGC 1 cut(s) 1691
BspPI GGATC 1 cut(s) 1512
BspTI CTTAAG 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 1422
BsrFI RCCGGY 1 cut(s) 462
BsrGI TGTACA 1 cut(s) 921
BsrI ACTGG 8 cut(s) 34, 250, 637, 713, 1286, 1565, 1656, 1698
BssAI RCCGGY 1 cut(s) 462
BssECI CCNNGG 3 cut(s) 671, 1149, 1214
BssMI GATC 5 cut(s) 117, 180, 336, 1504, 1648
BssNAI GTATAC 1 cut(s) 1476
BssNI GRCGYC 2 cut(s) 354, 1113
BssT1I CCWWGG 1 cut(s) 1214
Bst1107I GTATAC 1 cut(s) 1476
Bst2UI CCWGG 2 cut(s) 646, 672
Bst4CI ACNGT 3 cut(s) 235, 500, 1523
Bst6I CTCTTC 1 cut(s) 644
BstACI GRCGYC 2 cut(s) 354, 1113
BstAFI CTTAAG 1 cut(s) 316
BstAUI TGTACA 1 cut(s) 921
BstDEI CTNAG 4 cut(s) 45, 528, 602, 787
BstDSI CCRYGG 2 cut(s) 1149, 1214
BstENI CCTNNNNNAGG 1 cut(s) 531
BstF5I GGATG 5 cut(s) 197, 496, 1126, 1519, 1675
BstKTI GATC 5 cut(s) 120, 183, 339, 1507, 1651
BstMBI GATC 5 cut(s) 117, 180, 336, 1504, 1648
BstMWI GCNNNNNNNGC 3 cut(s) 353, 523, 1671
BstNI CCWGG 2 cut(s) 646, 672
BstNSI RCATGY 1 cut(s) 1588
BstSCI CCNGG 4 cut(s) 393, 399, 644, 670
BstSFI CTRYAG 2 cut(s) 1388, 1684
BstSLI GKGCMC 1 cut(s) 59
BstV1I GCAGC 1 cut(s) 1698
BstV2I GAAGAC 2 cut(s) 159, 545
BstX2I RGATCY 1 cut(s) 1504
BstYI RGATCY 1 cut(s) 1504
BstZ17I GTATAC 1 cut(s) 1476
Bsu36I CCTNAGG 1 cut(s) 528
BsuRI GGCC 5 cut(s) 43, 57, 428, 526, 1342
BtgI CCRYGG 2 cut(s) 1149, 1214
BtsCI GGATG 5 cut(s) 197, 496, 1126, 1519, 1675
BtsI GCAGTG 1 cut(s) 1689
BtsIMutI CAGTG 5 cut(s) 231, 346, 1528, 1663, 1689
BveI ACCTGC 1 cut(s) 1691
Cfr10I RCCGGY 1 cut(s) 462
Cfr13I GGNCC 7 cut(s) 12, 55, 56, 380, 397, 426, 1146
CseI GACGC 3 cut(s) 353, 362, 1019
Csp6I GTAC 6 cut(s) 597, 625, 784, 922, 1498, 1531
CspAI ACCGGT 1 cut(s) 462
CviAII CATG 7 cut(s) 259, 796, 823, 892, 919, 1215, 1585
CviQI GTAC 6 cut(s) 597, 625, 784, 922, 1498, 1531
DdeI CTNAG 4 cut(s) 45, 528, 602, 787
DpnI GATC 5 cut(s) 119, 182, 338, 1506, 1650
DpnII GATC 5 cut(s) 117, 180, 336, 1504, 1648
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
Eam1104I CTCTTC 1 cut(s) 644
EarI CTCTTC 1 cut(s) 644
Eco130I CCWWGG 1 cut(s) 1214
Eco147I AGGCCT 2 cut(s) 43, 526
Eco24I GRGCYC 1 cut(s) 59
Eco32I GATATC 1 cut(s) 451
Eco47I GGWCC 4 cut(s) 12, 380, 397, 1146
Eco57I CTGAAG 2 cut(s) 171, 780
Eco81I CCTNAGG 1 cut(s) 528
EcoNI CCTNNNNNAGG 1 cut(s) 531
EcoO109I RGGNCCY 2 cut(s) 380, 397
EcoRI GAATTC 1 cut(s) 703
EcoRII CCWGG 2 cut(s) 644, 670
EcoRV GATATC 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 1214
EcoT38I GRGCYC 1 cut(s) 59
ErhI CCWWGG 1 cut(s) 1214
FaeI CATG 7 cut(s) 262, 799, 826, 895, 922, 1218, 1588
FaqI GGGAC 2 cut(s) 366, 410
FatI CATG 7 cut(s) 258, 795, 822, 891, 918, 1214, 1584
FauI CCCGC 1 cut(s) 474
FblI GTMKAC 1 cut(s) 1475
Fnu4HI GCNGC 2 cut(s) 135, 1687
FokI GGATG 5 cut(s) 184, 503, 1133, 1526, 1682
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 2 cut(s) 135, 1687
FspBI CTAG 1 cut(s) 369
GluI GCNGC 2 cut(s) 135, 1687
GsuI CTGGAG 1 cut(s) 654
HaeIII GGCC 5 cut(s) 43, 57, 428, 526, 1342
HapII CCGG 4 cut(s) 394, 401, 463, 746
HgaI GACGC 3 cut(s) 353, 362, 1019
Hin1I GRCGYC 2 cut(s) 354, 1113
Hin1II CATG 7 cut(s) 262, 799, 826, 895, 922, 1218, 1588
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HindIII AAGCTT 2 cut(s) 34, 1547
HinfI GANTC 7 cut(s) 661, 755, 934, 992, 1063, 1196, 1292
HpaI GTTAAC 1 cut(s) 313
HpaII CCGG 4 cut(s) 394, 401, 463, 746
Hpy166II GTNNAC 5 cut(s) 313, 573, 793, 924, 1476
Hpy188I TCNGA 8 cut(s) 110, 226, 352, 490, 589, 666, 760, 1030
Hpy188III TCNNGA 5 cut(s) 24, 241, 938, 1236, 1615
Hpy8I GTNNAC 5 cut(s) 313, 573, 793, 924, 1476
Hpy99I CGWCG 2 cut(s) 362, 934
HpyCH4III ACNGT 3 cut(s) 235, 500, 1523
HpyCH4IV ACGT 2 cut(s) 95, 1113
HpyF10VI GCNNNNNNNGC 3 cut(s) 353, 523, 1671
HpyF3I CTNAG 4 cut(s) 45, 528, 602, 787
HpySE526I ACGT 2 cut(s) 95, 1113
Hsp92I GRCGYC 2 cut(s) 354, 1113
Hsp92II CATG 7 cut(s) 262, 799, 826, 895, 922, 1218, 1588
KflI GGGWCCC 2 cut(s) 380, 397
KspAI GTTAAC 1 cut(s) 313
Kzo9I GATC 5 cut(s) 117, 180, 336, 1504, 1648
LmnI GCTCC 3 cut(s) 268, 1153, 1333
Lsp1109I GCAGC 1 cut(s) 1698
LweI GCATC 5 cut(s) 371, 1007, 1111, 1342, 1683
MaeI CTAG 1 cut(s) 369
MaeII ACGT 2 cut(s) 95, 1113
MaeIII GTNAC 4 cut(s) 341, 611, 1090, 1580
MalI GATC 5 cut(s) 119, 182, 338, 1506, 1650
MboI GATC 5 cut(s) 117, 180, 336, 1504, 1648
MflI RGATCY 1 cut(s) 1504
MhlI GDGCHC 2 cut(s) 59, 108
MlyI GAGTC 3 cut(s) 764, 1001, 1205
MroXI GAANNNNTTC 1 cut(s) 956
MseI TTAA 8 cut(s) 312, 317, 444, 986, 1107, 1244, 1551, 1702
MslI CAYNNNNRTG 2 cut(s) 1097, 1589
MspCI CTTAAG 1 cut(s) 316
MspI CCGG 4 cut(s) 394, 401, 463, 746
MspR9I CCNGG 4 cut(s) 395, 401, 646, 672
MvaI CCWGG 2 cut(s) 646, 672
MwoI GCNNNNNNNGC 3 cut(s) 353, 523, 1671
NciI CCSGG 2 cut(s) 395, 401
NcoI CCATGG 1 cut(s) 1214
NdeII GATC 5 cut(s) 117, 180, 336, 1504, 1648
NlaIII CATG 7 cut(s) 262, 799, 826, 895, 922, 1218, 1588
NlaIV GGNNCC 9 cut(s) 57, 264, 381, 382, 398, 399, 1147, 1155, 1415
NmuCI GTSAC 3 cut(s) 341, 611, 1090
NspI RCATGY 1 cut(s) 1588
PaqCI CACCTGC 1 cut(s) 1691
PceI AGGCCT 2 cut(s) 43, 526
PdmI GAANNNNTTC 1 cut(s) 956
PfeI GAWTC 4 cut(s) 661, 934, 1063, 1292
PflFI GACNNNGTC 1 cut(s) 356
PinAI ACCGGT 1 cut(s) 462
PkrI GCNGC 2 cut(s) 136, 1688
PleI GAGTC 3 cut(s) 763, 1000, 1204
PpsI GAGTC 3 cut(s) 763, 1000, 1204
PpuMI RGGWCCY 2 cut(s) 380, 397
Psp5II RGGWCCY 2 cut(s) 380, 397
Psp6I CCWGG 2 cut(s) 644, 670
PspGI CCWGG 2 cut(s) 644, 670
PspN4I GGNNCC 9 cut(s) 57, 264, 381, 382, 398, 399, 1147, 1155, 1415
PspOMI GGGCCC 1 cut(s) 55
PspPI GGNCC 7 cut(s) 12, 55, 56, 380, 397, 426, 1146
PspPPI RGGWCCY 2 cut(s) 380, 397
PstI CTGCAG 2 cut(s) 1392, 1688
PsuI RGATCY 1 cut(s) 1504
PsyI GACNNNGTC 1 cut(s) 356
RsaI GTAC 6 cut(s) 598, 626, 785, 923, 1499, 1532
RsaNI GTAC 6 cut(s) 597, 625, 784, 922, 1498, 1531
RseI CAYNNNNRTG 2 cut(s) 1097, 1589
SaqAI TTAA 8 cut(s) 312, 317, 444, 986, 1107, 1244, 1551, 1702
SatI GCNGC 2 cut(s) 135, 1687
Sau3AI GATC 5 cut(s) 117, 180, 336, 1504, 1648
Sau96I GGNCC 7 cut(s) 12, 55, 56, 380, 397, 426, 1146
SbfI CCTGCAGG 1 cut(s) 1392
ScaI AGTACT 1 cut(s) 785
SchI GAGTC 3 cut(s) 764, 1001, 1205
ScrFI CCNGG 4 cut(s) 395, 401, 646, 672
SdaI CCTGCAGG 1 cut(s) 1392
SduI GDGCHC 2 cut(s) 59, 108
SfaNI GCATC 5 cut(s) 371, 1007, 1111, 1342, 1683
SfcI CTRYAG 2 cut(s) 1388, 1684
SinI GGWCC 4 cut(s) 12, 380, 397, 1146
SmiMI CAYNNNNRTG 2 cut(s) 1097, 1589
SmlI CTYRAG 2 cut(s) 316, 1615
SmoI CTYRAG 2 cut(s) 316, 1615
Sse8387I CCTGCAGG 1 cut(s) 1392
SseBI AGGCCT 2 cut(s) 43, 526
SsiI CCGC 3 cut(s) 135, 481, 517
SspI AATATT 1 cut(s) 904
SspMI CTAG 1 cut(s) 369
StuI AGGCCT 2 cut(s) 43, 526
StyD4I CCNGG 4 cut(s) 393, 399, 644, 670
StyI CCWWGG 1 cut(s) 1214
TaaI ACNGT 3 cut(s) 235, 500, 1523
TaiI ACGT 2 cut(s) 98, 1116
TaqI TCGA 3 cut(s) 932, 1235, 1536
TatI WGTACW 4 cut(s) 596, 783, 921, 1497
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 4 cut(s) 661, 934, 1063, 1292
Tru1I TTAA 8 cut(s) 312, 317, 444, 986, 1107, 1244, 1551, 1702
Tru9I TTAA 8 cut(s) 312, 317, 444, 986, 1107, 1244, 1551, 1702
TscAI CASTG 5 cut(s) 238, 346, 1528, 1663, 1696
TseFI GTSAC 3 cut(s) 341, 611, 1090
TseI GCWGC 1 cut(s) 1686
Tsp45I GTSAC 3 cut(s) 341, 611, 1090
TspDTI ATGAA 7 cut(s) 159, 247, 756, 839, 1058, 1115, 1530
TspGWI ACGGA 2 cut(s) 1138, 1208
TspRI CASTG 5 cut(s) 238, 346, 1528, 1663, 1696
Tth111I GACNNNGTC 1 cut(s) 356
Vha464I CTTAAG 1 cut(s) 316
VpaK11BI GGWCC 4 cut(s) 12, 380, 397, 1146
XagI CCTNNNNNAGG 1 cut(s) 531
XapI RAATTY 5 cut(s) 49, 253, 278, 703, 1571
XceI RCATGY 1 cut(s) 1588
XcmI CCANNNNNNNNNTGG 1 cut(s) 1164
XmiI GTMKAC 1 cut(s) 1475
XmnI GAANNNNTTC 1 cut(s) 956
XspI CTAG 1 cut(s) 369
ZraI GACGTC 1 cut(s) 1114
ZrmI AGTACT 1 cut(s) 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.