pycom09g05320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0012448
Physical Location & Seq
Forward (+)
3956103 .. 3957530
1428 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g05320.1

Sequence Viewer

Length: 1428 bp
ATGACTGATGTTGTTAACTTGAGTGAGGGCGATAACTCGATCACTCAGAATTCTGAAGCAGAATCTGCTAGTTGTGAGGGTCCCATTGATTGCAGGGACCCGGAGGTGTTCCATTTGATGATGAGGGCCACCATAGAGAAGTTTAAGGATATTCATTTTTACCGGTTTGGGAAACCAGTGCAGGGTTCAGATGGTAACAGTTGTGATATGGCATGGCGGTTTAGGCCTAAGGAAGGGAAGACTGCTTCTTTTTACAAGGACTATCGGAGATTTAGCATTTTGAAGTCTGAGAATTGTACTCTTAGTGTGGGTGATATTGGAGAGTACCATACTGGAGTAAATGCCAGGAAGAGGAAGAAGAAGGCCGGGTTTGATAAGCAAGGGAAGACAGAGGCCGGGTTTGATAAGCAAGGGAAGACAGAGATGAATTCTTTGCCAGTTGTTGGGGAGGTTGTGAATGATACCCTACCGGTGGTTGAGTCTGAAGGTTCATTCAGTCATGGGAAGTACTTAATTTATAGTGGTGGTGGAGATAGGTGCAAGAGCATGAACCATTACTTGTGGAGTTTCCTGTGTGCTTTAGGTGAAGCTCAGTACCTGAACCGAACTTTGGTCATGGAATTGACTCTTTGTCTGTCTTCGATTTACACTTCGTCGAATCAAGATGAGGAAGGGAAGGATTTCAGGTTTTACTTTGACTTTGAGCATTTAAGAGAGTCTGCATCTGTATTAGATGCAAAGCAGTTTTGGACAGACTGGGAAAAATGGCAGAAGAAAGACGGTTTGGGTATGCATCTTGTAGAGGATTATAGGGTCACACCTATGAAACTTAATGATGTCAAGGATACTTTGATTATGAGAAAGTTTGGATCTGTGGAGCCAGACAATTATTGGTATAGGGTGTGTGAAGGGGAGACAGAGTCCGTAGTACAACGACCATGGCATTTGTTGTGGAAATCGAGAAGGTTGATGGATGTAGTGTCTGCAATTGCATCAAAGTTGAACTGGGATTATGACTCTGTGCACATTGAGAGAGGGGAGAAAGCACAGAACAAGGAGCTTTGGCCTAACCTTGATGCAGATACTTCACCTAGTGCACTTCTCTCAACCCTGCCAAACAAAATTGAAGACGGAAGGAACCTCTACATTGCAACAAACGAGCCAGATACATCTTTCTTTGACCCTTTGAAAGACAAGTATACCACTCATTTCCTCGACGAGTACAAGGATCTTTGGGACGAAAGCAGTGAGTGGTACTTTGATACCACTAGGCTCAACAATGGAGTCCCTGTTGAATTTGACGGTTACATGAGGGTCTCGGTTGACACAGAGGTATTCTTGAGAGGGAAAAAGCAGATAGAAACTTTCAACGATCTCACCAAAGACTGCAAGGATGGTATCAACAGTTGCAGCACCGCGACCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

476

Amino Acids

54.63

Weight (kDa)

5.19

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7074 PF23269 27 - 111 3.2e-39 Domain of unknown function (DUF7074)
DUF7075 PF23272 164 - 455 3.3e-88 Domain of unknown function (DUF7075)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012448)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04280 AT4G12700
fragaria_vesca FvH4_6g40420
malus_domestica MD09G1130100.v1.1 MD17G1105300.v1.1
prunus_persica Prupe.3G195000_v2.0.a1 Prupe.3G195000_v2.0.a1
pyrus_communis pycom09g05320
rosa_chinensis RchiOBHm_Chr2g0155651
rosa_laevigata RLG00000020851
rosa_multiflora Rmu_ssc0000430.1_g000005
rosa_roxburghii Rroxscaffold_1G00014560 Rroxscaffold_2G00093590
rosa_rugosa Rorug02G0448800
rosa_samantha Rh2AG514900 Rh2BG525800 Rh2CG499800 Rh2DG534700
rosa_wichuraiana Rw2G042310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 443
AccI GTMKAC 1 cut(s) 1199
AccII CGCG 1 cut(s) 1418
AciI CCGC 2 cut(s) 217, 1416
AclWI GGATC 2 cut(s) 877, 1236
AcsI RAATTY 3 cut(s) 49, 427, 1295
AcuI CTGAAG 2 cut(s) 75, 504
AdeI CACNNNGTG 1 cut(s) 1094
AfaI GTAC 7 cut(s) 298, 326, 509, 596, 930, 1223, 1256
AfiI CCNNNNNNNGG 6 cut(s) 182, 233, 351, 443, 472, 610
AgeI ACCGGT 2 cut(s) 162, 469
AgsI TTSAA 6 cut(s) 283, 1003, 1127, 1189, 1295, 1369
AhdI GACNNNNNGTC 1 cut(s) 630
AjnI CCWGG 1 cut(s) 344
AjuI GAANNNNNNNTTGG 4 cut(s) 593, 625, 767, 799
AluBI AGCT 3 cut(s) 590, 1060, 1425
AluI AGCT 3 cut(s) 590, 1060, 1425
Alw21I GWGCWC 2 cut(s) 1026, 1099
Alw26I GTCTC 2 cut(s) 908, 1321
Alw44I GTGCAC 2 cut(s) 1022, 1095
AlwI GGATC 2 cut(s) 877, 1236
AlwNI CAGNNNCTG 2 cut(s) 65, 598
AoxI GGCC 5 cut(s) 126, 224, 363, 393, 1064
ApaLI GTGCAC 2 cut(s) 1022, 1095
ApeKI GCWGC 1 cut(s) 1410
ApoI RAATTY 3 cut(s) 49, 427, 1295
AsiGI ACCGGT 2 cut(s) 162, 469
Asp700I GAANNNNTTC 1 cut(s) 680
AspS9I GGNCC 3 cut(s) 80, 97, 126
AsuC2I CCSGG 3 cut(s) 101, 367, 397
AsuHPI GGTGA 4 cut(s) 323, 596, 1080, 1369
AvaII GGWCC 2 cut(s) 80, 97
AxyI CCTNAGG 1 cut(s) 228
BaeGI GKGCMC 2 cut(s) 1026, 1099
BbsI GAAGAC 5 cut(s) 245, 392, 422, 630, 1134
Bbv12I GWGCWC 2 cut(s) 1026, 1099
BbvI GCAGC 1 cut(s) 1422
BccI CCATC 3 cut(s) 185, 964, 1388
BciT130I CCWGG 1 cut(s) 346
BciVI GTATCC 1 cut(s) 838
BcnI CCSGG 3 cut(s) 101, 367, 397
BcoDI GTCTC 2 cut(s) 908, 1321
BfaI CTAG 3 cut(s) 69, 1092, 1269
BfuI GTATCC 1 cut(s) 838
BisI GCNGC 1 cut(s) 1411
BlsI GCNGC 1 cut(s) 1412
BmcAI AGTACT 1 cut(s) 509
Bme1390I CCNGG 4 cut(s) 101, 346, 367, 397
Bme18I GGWCC 2 cut(s) 80, 97
BmeRI GACNNNNNGTC 1 cut(s) 630
BmgT120I GGNCC 3 cut(s) 80, 97, 126
BmiI GGNNCC 6 cut(s) 81, 82, 98, 99, 879, 1139
BmrFI CCNGG 4 cut(s) 101, 346, 367, 397
BmrI ACTGGG 2 cut(s) 766, 1015
BmsI GCATC 5 cut(s) 724, 731, 802, 1001, 1066
BmuI ACTGGG 2 cut(s) 766, 1015
BpiI GAAGAC 5 cut(s) 245, 392, 422, 630, 1134
BpmI CTGGAG 1 cut(s) 354
BpuEI CTTGAG 2 cut(s) 40, 1360
BpuMI CCSGG 3 cut(s) 101, 367, 397
BsaI GGTCTC 1 cut(s) 1321
BsaJI CCNNGG 1 cut(s) 938
BsaWI WCCGGW 2 cut(s) 162, 469
BsaXI ACNNNNNCTCC 4 cut(s) 905, 935, 1275, 1305
Bsc4I CCNNNNNNNGG 6 cut(s) 182, 233, 351, 443, 472, 610
Bse118I RCCGGY 2 cut(s) 162, 469
Bse1I ACTGG 5 cut(s) 176, 337, 437, 761, 1010
Bse21I CCTNAGG 1 cut(s) 228
Bse3DI GCAATG 1 cut(s) 1146
BseBI CCWGG 1 cut(s) 346
BseDI CCNNGG 1 cut(s) 938
BseGI GGATG 2 cut(s) 979, 1399
BseLI CCNNNNNNNGG 6 cut(s) 182, 233, 351, 443, 472, 610
BseMI GCAATG 1 cut(s) 1146
BseMII CTCAG 3 cut(s) 59, 279, 605
BseNI ACTGG 5 cut(s) 176, 337, 437, 761, 1010
BseSI GKGCMC 2 cut(s) 1026, 1099
BseXI GCAGC 1 cut(s) 1422
BsgI GTGCAG 1 cut(s) 200
Bsh1236I CGCG 1 cut(s) 1418
BshFI GGCC 5 cut(s) 128, 226, 365, 395, 1066
BshTI ACCGGT 2 cut(s) 162, 469
BsiHKAI GWGCWC 2 cut(s) 1026, 1099
BsiSI CCGG 5 cut(s) 101, 163, 366, 396, 470
BslFI GGGAC 4 cut(s) 66, 110, 1250, 1271
BslI CCNNNNNNNGG 6 cut(s) 182, 233, 351, 443, 472, 610
BsmAI GTCTC 2 cut(s) 908, 1321
BsmFI GGGAC 4 cut(s) 66, 110, 1250, 1271
BsnI GGCC 5 cut(s) 128, 226, 365, 395, 1066
Bso31I GGTCTC 1 cut(s) 1321
Bsp1286I GDGCHC 2 cut(s) 1026, 1099
Bsp143I GATC 4 cut(s) 39, 869, 1228, 1372
Bsp19I CCATGG 1 cut(s) 938
BspACI CCGC 2 cut(s) 217, 1416
BspANI GGCC 5 cut(s) 128, 226, 365, 395, 1066
BspCNI CTCAG 3 cut(s) 58, 280, 604
BspFNI CGCG 1 cut(s) 1418
BspLI GGNNCC 6 cut(s) 81, 82, 98, 99, 879, 1139
BspPI GGATC 2 cut(s) 877, 1236
BspTNI GGTCTC 1 cut(s) 1321
BsrDI GCAATG 1 cut(s) 1146
BsrFI RCCGGY 2 cut(s) 162, 469
BsrI ACTGG 5 cut(s) 176, 337, 437, 761, 1010
BssAI RCCGGY 2 cut(s) 162, 469
BssECI CCNNGG 1 cut(s) 938
BssMI GATC 4 cut(s) 39, 869, 1228, 1372
BssNAI GTATAC 1 cut(s) 1200
BssT1I CCWWGG 1 cut(s) 938
Bst1107I GTATAC 1 cut(s) 1200
Bst2UI CCWGG 1 cut(s) 346
Bst4CI ACNGT 4 cut(s) 200, 782, 1304, 1406
Bst6I CTCTTC 1 cut(s) 344
BstAPI GCANNNNNTGC 1 cut(s) 65
BstDEI CTNAG 5 cut(s) 45, 228, 288, 302, 591
BstDSI CCRYGG 1 cut(s) 938
BstENI CCTNNNNNAGG 1 cut(s) 231
BstF5I GGATG 2 cut(s) 979, 1399
BstFNI CGCG 1 cut(s) 1418
BstKTI GATC 4 cut(s) 42, 872, 1231, 1375
BstMAI GTCTC 2 cut(s) 908, 1321
BstMBI GATC 4 cut(s) 39, 869, 1228, 1372
BstMWI GCNNNNNNNGC 2 cut(s) 65, 223
BstNI CCWGG 1 cut(s) 346
BstSCI CCNGG 4 cut(s) 99, 344, 365, 395
BstSLI GKGCMC 2 cut(s) 1026, 1099
BstUI CGCG 1 cut(s) 1418
BstV1I GCAGC 1 cut(s) 1422
BstV2I GAAGAC 5 cut(s) 245, 392, 422, 630, 1134
BstX2I RGATCY 2 cut(s) 869, 1228
BstYI RGATCY 2 cut(s) 869, 1228
BstZ17I GTATAC 1 cut(s) 1200
Bsu36I CCTNAGG 1 cut(s) 228
BsuI GTATCC 1 cut(s) 838
BsuRI GGCC 5 cut(s) 128, 226, 365, 395, 1066
BtgI CCRYGG 1 cut(s) 938
BtsCI GGATG 2 cut(s) 979, 1399
BtsI GCAGTG 1 cut(s) 1252
BtsIMutI CAGTG 2 cut(s) 183, 1252
CaiI CAGNNNCTG 2 cut(s) 65, 598
Cfr10I RCCGGY 2 cut(s) 162, 469
Cfr13I GGNCC 3 cut(s) 80, 97, 126
Csp6I GTAC 7 cut(s) 297, 325, 508, 595, 929, 1222, 1255
CspAI ACCGGT 2 cut(s) 162, 469
CviAII CATG 6 cut(s) 213, 500, 547, 616, 939, 1309
CviQI GTAC 7 cut(s) 297, 325, 508, 595, 929, 1222, 1255
DdeI CTNAG 5 cut(s) 45, 228, 288, 302, 591
DpnI GATC 4 cut(s) 41, 871, 1230, 1374
DpnII GATC 4 cut(s) 39, 869, 1228, 1372
DraIII CACNNNGTG 1 cut(s) 1094
DriI GACNNNNNGTC 1 cut(s) 630
Eam1104I CTCTTC 1 cut(s) 344
Eam1105I GACNNNNNGTC 1 cut(s) 630
EarI CTCTTC 1 cut(s) 344
Eco130I CCWWGG 1 cut(s) 938
Eco147I AGGCCT 1 cut(s) 226
Eco31I GGTCTC 1 cut(s) 1321
Eco47I GGWCC 2 cut(s) 80, 97
Eco57I CTGAAG 2 cut(s) 75, 504
Eco81I CCTNAGG 1 cut(s) 228
EcoNI CCTNNNNNAGG 1 cut(s) 231
EcoO109I RGGNCCY 2 cut(s) 80, 97
EcoRI GAATTC 2 cut(s) 49, 427
EcoRII CCWGG 1 cut(s) 344
EcoT14I CCWWGG 1 cut(s) 938
EcoT22I ATGCAT 1 cut(s) 795
ErhI CCWWGG 1 cut(s) 938
FaeI CATG 6 cut(s) 216, 503, 550, 619, 942, 1312
FaqI GGGAC 4 cut(s) 66, 110, 1250, 1271
FatI CATG 6 cut(s) 212, 499, 546, 615, 938, 1308
FblI GTMKAC 1 cut(s) 1199
Fnu4HI GCNGC 1 cut(s) 1411
FokI GGATG 2 cut(s) 986, 1406
Fsp4HI GCNGC 1 cut(s) 1411
FspBI CTAG 3 cut(s) 69, 1092, 1269
GluI GCNGC 1 cut(s) 1411
GsuI CTGGAG 1 cut(s) 354
HaeIII GGCC 5 cut(s) 128, 226, 365, 395, 1066
HapII CCGG 5 cut(s) 101, 163, 366, 396, 470
Hin1II CATG 6 cut(s) 216, 503, 550, 619, 942, 1312
HincII GTYRAC 2 cut(s) 16, 1324
HindII GTYRAC 2 cut(s) 16, 1324
HinfI GANTC 8 cut(s) 62, 479, 625, 658, 716, 920, 1016, 1284
HpaI GTTAAC 1 cut(s) 16
HpaII CCGG 5 cut(s) 101, 163, 366, 396, 470
HphI GGTGA 4 cut(s) 323, 596, 1080, 1369
Hpy166II GTNNAC 5 cut(s) 16, 1024, 1097, 1200, 1324
Hpy188I TCNGA 6 cut(s) 48, 55, 190, 267, 289, 484
Hpy188III TCNNGA 3 cut(s) 662, 960, 1339
Hpy8I GTNNAC 5 cut(s) 16, 1024, 1097, 1200, 1324
Hpy99I CGWCG 2 cut(s) 658, 1220
HpyAV CCTTC 8 cut(s) 227, 355, 479, 665, 670, 902, 957, 1128
HpyCH4III ACNGT 4 cut(s) 200, 782, 1304, 1406
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 223
HpyF3I CTNAG 5 cut(s) 45, 228, 288, 302, 591
Hsp92II CATG 6 cut(s) 216, 503, 550, 619, 942, 1312
KflI GGGWCCC 2 cut(s) 80, 97
KspAI GTTAAC 1 cut(s) 16
Kzo9I GATC 4 cut(s) 39, 869, 1228, 1372
LmnI GCTCC 2 cut(s) 877, 1057
Lsp1109I GCAGC 1 cut(s) 1422
LweI GCATC 5 cut(s) 724, 731, 802, 1001, 1066
MaeI CTAG 3 cut(s) 69, 1092, 1269
MaeIII GTNAC 3 cut(s) 194, 814, 1304
MalI GATC 4 cut(s) 41, 871, 1230, 1374
MboI GATC 4 cut(s) 39, 869, 1228, 1372
MboII GAAGA 9 cut(s) 250, 361, 367, 370, 397, 427, 630, 784, 1139
MfeI CAATTG 1 cut(s) 987
MflI RGATCY 2 cut(s) 869, 1228
MhlI GDGCHC 2 cut(s) 1026, 1099
MluCI AATT 9 cut(s) 49, 292, 427, 513, 620, 886, 987, 1122, 1295
MlyI GAGTC 6 cut(s) 488, 619, 725, 929, 1010, 1293
Mph1103I ATGCAT 1 cut(s) 795
MroXI GAANNNNTTC 1 cut(s) 680
MseI TTAA 5 cut(s) 15, 144, 512, 710, 831
MslI CAYNNNNRTG 1 cut(s) 821
MspA1I CMGCKG 1 cut(s) 1425
MspI CCGG 5 cut(s) 101, 163, 366, 396, 470
MspR9I CCNGG 4 cut(s) 101, 346, 367, 397
MunI CAATTG 1 cut(s) 987
MvaI CCWGG 1 cut(s) 346
MvnI CGCG 1 cut(s) 1418
MwoI GCNNNNNNNGC 2 cut(s) 65, 223
NciI CCSGG 3 cut(s) 101, 367, 397
NcoI CCATGG 1 cut(s) 938
NdeII GATC 4 cut(s) 39, 869, 1228, 1372
NlaIII CATG 6 cut(s) 216, 503, 550, 619, 942, 1312
NlaIV GGNNCC 6 cut(s) 81, 82, 98, 99, 879, 1139
NmuCI GTSAC 1 cut(s) 814
NsiI ATGCAT 1 cut(s) 795
PceI AGGCCT 1 cut(s) 226
PdmI GAANNNNTTC 1 cut(s) 680
PfeI GAWTC 2 cut(s) 62, 658
PflFI GACNNNGTC 1 cut(s) 919
PflMI CCANNNNNTGG 1 cut(s) 443
PinAI ACCGGT 2 cut(s) 162, 469
PkrI GCNGC 1 cut(s) 1412
PleI GAGTC 6 cut(s) 487, 619, 724, 928, 1010, 1292
PpsI GAGTC 6 cut(s) 487, 619, 724, 928, 1010, 1292
PpuMI RGGWCCY 2 cut(s) 80, 97
Psp5II RGGWCCY 2 cut(s) 80, 97
Psp6I CCWGG 1 cut(s) 344
PspGI CCWGG 1 cut(s) 344
PspN4I GGNNCC 6 cut(s) 81, 82, 98, 99, 879, 1139
PspPI GGNCC 3 cut(s) 80, 97, 126
PspPPI RGGWCCY 2 cut(s) 80, 97
PstNI CAGNNNCTG 2 cut(s) 65, 598
PsuI RGATCY 2 cut(s) 869, 1228
PsyI GACNNNGTC 1 cut(s) 919
PvuII CAGCTG 1 cut(s) 1425
RsaI GTAC 7 cut(s) 298, 326, 509, 596, 930, 1223, 1256
RsaNI GTAC 7 cut(s) 297, 325, 508, 595, 929, 1222, 1255
RseI CAYNNNNRTG 1 cut(s) 821
SaqAI TTAA 5 cut(s) 15, 144, 512, 710, 831
SatI GCNGC 1 cut(s) 1411
Sau3AI GATC 4 cut(s) 39, 869, 1228, 1372
Sau96I GGNCC 3 cut(s) 80, 97, 126
ScaI AGTACT 1 cut(s) 509
SchI GAGTC 6 cut(s) 488, 619, 725, 929, 1010, 1293
ScrFI CCNGG 4 cut(s) 101, 346, 367, 397
SduI GDGCHC 2 cut(s) 1026, 1099
SfaNI GCATC 5 cut(s) 724, 731, 802, 1001, 1066
SinI GGWCC 2 cut(s) 80, 97
SmiMI CAYNNNNRTG 1 cut(s) 821
SmlI CTYRAG 2 cut(s) 19, 1339
SmoI CTYRAG 2 cut(s) 19, 1339
Sse9I AATT 9 cut(s) 49, 292, 427, 513, 620, 886, 987, 1122, 1295
SseBI AGGCCT 1 cut(s) 226
SsiI CCGC 2 cut(s) 217, 1416
SspMI CTAG 3 cut(s) 69, 1092, 1269
StuI AGGCCT 1 cut(s) 226
StyD4I CCNGG 4 cut(s) 99, 344, 365, 395
StyI CCWWGG 1 cut(s) 938
TaaI ACNGT 4 cut(s) 200, 782, 1304, 1406
TaqI TCGA 5 cut(s) 38, 641, 656, 959, 1215
TasI AATT 9 cut(s) 49, 292, 427, 513, 620, 886, 987, 1122, 1295
TatI WGTACW 4 cut(s) 296, 507, 928, 1221
TfiI GAWTC 2 cut(s) 62, 658
Tru1I TTAA 5 cut(s) 15, 144, 512, 710, 831
Tru9I TTAA 5 cut(s) 15, 144, 512, 710, 831
TscAI CASTG 2 cut(s) 183, 1252
TseFI GTSAC 1 cut(s) 814
TseI GCWGC 1 cut(s) 1410
Tsp45I GTSAC 1 cut(s) 814
TspDTI ATGAA 5 cut(s) 143, 440, 480, 563, 839
TspGWI ACGGA 2 cut(s) 913, 1146
TspRI CASTG 2 cut(s) 183, 1252
Tth111I GACNNNGTC 1 cut(s) 919
Van91I CCANNNNNTGG 1 cut(s) 443
VneI GTGCAC 2 cut(s) 1022, 1095
VpaK11BI GGWCC 2 cut(s) 80, 97
XagI CCTNNNNNAGG 1 cut(s) 231
XapI RAATTY 3 cut(s) 49, 427, 1295
XcmI CCANNNNNNNNNTGG 1 cut(s) 888
XmiI GTMKAC 1 cut(s) 1199
XmnI GAANNNNTTC 1 cut(s) 680
XspI CTAG 3 cut(s) 69, 1092, 1269
ZrmI AGTACT 1 cut(s) 509
Zsp2I ATGCAT 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.