AT4G13700

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004573
Physical Location & Seq
Reverse (-)
7957072 .. 7958919
1848 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G13700.2

Sequence Viewer

Length: 1377 bp
ATGACTTTACTAATAATGATTACCCTAACCTCAATATCATTGTTACTTGCCGCCGCCGAAACAATCCCGACAACTTTAGACGGCCCGTTCAAGCCATTAACCCGCCGGTTCGAGCCGTCTCTACGACGAGGCAGTGACGACTTACCTATGGATCATCCAAGGCTGAGAAAGAGAAACGTTAGCTCCGATTTTCCAGAACAGATTGCTTTAGCTCTCTCTACACCAACCTCCATGTGGGTTTCTTGGGTCACTGGTGATGCTATAGTTGGCAAAGATGTGAAACCGCTTGATCCTAGTTCTATTGCTAGTGAGGTTTGGTATGGGAAGGAGAAAGGAAACTATATGCTAAAGAAGAAAGGAAACGCAACAGTTTACAGTCAGTTATATCCCTCTGATGGTCTTCTCAATTACACATCAGGCATCATACATCATGTCCTCATTGACGGTCTGGAACCAGAAACTAGGTATTATTATAGGTGTGGTGATAGCTCTGTTCCTGCAATGAGTGAGGAGATTTCTTTTGAGACTTTGCCATTGCCAAGTAAAGATGCATATCCTCATAGAATTGCCTTTGTTGGGGATTTGGGTCTTACTAGTAATACTACAACCACCATTGACCATTTGATGGAAAACGATCCCTCATTGGTTATAATTGTTGGGGACTTAACTTATGCAAACCAGTATCGTACTATTGGTGGTAAAGGAGTTCCATGCTTCTCATGTTCGTTTCCTGATGCACCTATAAGGGAGACTTATCAACCTCGCTGGGATGCTTGGGGAAGGTTCATGGAGCCATTGACTTCCAAGGTCCCAACGATGGTCATTGAAGGCAACCATGAGATTGAGCCTCAAGCTTCAGGGATCACCTTTAAGTCATATTCTGAAAGGTTTGCTGTTCCTGCAAGTGAGAGTGGCTCCAACAGCAACTTCTACTATTCTTTTGATGCCGGAGGAGTGCATTTTGTTATGTTGGGTGCATATGTTGATTACAATAACACTGGGCTGCAATATGCATGGCTAAAGGAAGACTTGTCTAAAGTTGATCGCGCTGTGACACCATGGCTGGTTGCAACTATGCATCCACCTTGGTACAACAGCTACTCCTCGCATTACCAGGAGTTTGAATGCATGAGGCAGGAAATGGAAGAGCTTCTTTACCAATACCGTGTTGACATAGTTTTTGCAGGACATGTACATGCATACGAGAGAATGAACCGCATATACAACTACACACTAGACCCATGTGGACCAGTTTACATAACAATAGGAGATGGTGGAAACATTGAGAAAGTTGATGTGGACTTTGCTGATGACCCTGGGAAGTGTCACTCTTCCTACGATCTTTTTTTTTTCAATTCTTTGAATCTTTCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

458

Amino Acids

51.58

Weight (kDa)

5.03

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_ac_phosph_N PF16656 65 - 176 5.7e-20 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 188 - 398 2.1e-19 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 650
AccB7I CCANNNNNTGG 1 cut(s) 625
AccII CGCG 1 cut(s) 1047
AciI CCGC 5 cut(s) 51, 54, 103, 284, 1216
AclI AACGTT 1 cut(s) 177
AclWI GGATC 4 cut(s) 159, 284, 629, 869
AcuI CTGAAG 1 cut(s) 840
AfaI GTAC 3 cut(s) 688, 1091, 1194
AfiI CCNNNNNNNGG 5 cut(s) 234, 395, 576, 625, 817
AflIII ACRYGT 1 cut(s) 1189
AgsI TTSAA 5 cut(s) 91, 827, 1124, 1354, 1363
AhlI ACTAGT 1 cut(s) 593
AjnI CCWGG 2 cut(s) 1113, 1315
AjuI GAANNNNNNNTTGG 2 cut(s) 911, 943
AluBI AGCT 6 cut(s) 183, 212, 489, 854, 1098, 1150
AluI AGCT 6 cut(s) 183, 212, 489, 854, 1098, 1150
Alw26I GTCTC 3 cut(s) 123, 518, 743
AlwI GGATC 4 cut(s) 159, 284, 629, 869
AoxI GGCC 1 cut(s) 82
ApeKI GCWGC 1 cut(s) 1003
ArsI GACNNNNNNTTYG 2 cut(s) 1163, 1195
Asp700I GAANNNNTTC 1 cut(s) 1149
AspLEI GCGC 1 cut(s) 1049
AspS9I GGNCC 3 cut(s) 83, 808, 1247
AsuHPI GGTGA 3 cut(s) 266, 494, 856
AvaII GGWCC 2 cut(s) 808, 1247
BbsI GAAGAC 2 cut(s) 392, 1032
BbvI GCAGC 1 cut(s) 990
BccI CCATC 4 cut(s) 389, 619, 811, 1265
BceAI ACGGC 2 cut(s) 97, 100
BciT130I CCWGG 2 cut(s) 1115, 1317
BcoDI GTCTC 3 cut(s) 123, 518, 743
BcuI ACTAGT 1 cut(s) 593
BfaI CTAG 5 cut(s) 294, 306, 462, 594, 1235
BfmI CTRYAG 1 cut(s) 261
BisI GCNGC 3 cut(s) 51, 54, 1004
BlsI GCNGC 3 cut(s) 52, 55, 1005
Bme1390I CCNGG 2 cut(s) 1115, 1317
Bme18I GGWCC 2 cut(s) 808, 1247
BmgT120I GGNCC 3 cut(s) 83, 808, 1247
BmiI GGNNCC 4 cut(s) 453, 792, 810, 916
BmrFI CCNGG 2 cut(s) 1115, 1317
BmrI ACTGGG 1 cut(s) 1008
BmsI GCATC 7 cut(s) 247, 429, 538, 724, 760, 934, 1087
BmuI ACTGGG 1 cut(s) 1008
BpiI GAAGAC 2 cut(s) 392, 1032
BplI GAGNNNNNCTC 2 cut(s) 899, 931
BpuEI CTTGAG 1 cut(s) 834
BsaBI GATNNNNATC 1 cut(s) 552
BsaJI CCNNGG 6 cut(s) 158, 804, 1058, 1085, 1315, 1316
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 1085, 1115
Bsc4I CCNNNNNNNGG 5 cut(s) 234, 395, 576, 625, 817
Bse118I RCCGGY 1 cut(s) 105
Bse1I ACTGG 4 cut(s) 256, 679, 1003, 1250
Bse3DI GCAATG 2 cut(s) 507, 533
Bse8I GATNNNNATC 1 cut(s) 552
BseBI CCWGG 2 cut(s) 1115, 1317
BseDI CCNNGG 6 cut(s) 158, 804, 1058, 1085, 1315, 1316
BseGI GGATG 3 cut(s) 154, 775, 1078
BseJI GATNNNNATC 1 cut(s) 552
BseLI CCNNNNNNNGG 5 cut(s) 234, 395, 576, 625, 817
BseMI GCAATG 2 cut(s) 507, 533
BseMII CTCAG 1 cut(s) 155
BseNI ACTGG 4 cut(s) 256, 679, 1003, 1250
BseRI GAGGAG 3 cut(s) 524, 966, 1093
BseXI GCAGC 1 cut(s) 990
BseYI CCCAGC 1 cut(s) 765
Bsh1236I CGCG 1 cut(s) 1047
BshFI GGCC 1 cut(s) 84
BsiSI CCGG 2 cut(s) 106, 948
BslFI GGGAC 2 cut(s) 674, 794
BslI CCNNNNNNNGG 5 cut(s) 234, 395, 576, 625, 817
BsmAI GTCTC 3 cut(s) 123, 518, 743
BsmBI CGTCTC 1 cut(s) 123
BsmFI GGGAC 2 cut(s) 674, 794
BsmI GAATGC 1 cut(s) 1130
BsnI GGCC 1 cut(s) 84
Bsp1407I TGTACA 1 cut(s) 1192
Bsp143I GATC 6 cut(s) 151, 289, 634, 861, 1042, 1339
Bsp19I CCATGG 1 cut(s) 1058
BspACI CCGC 5 cut(s) 51, 54, 103, 284, 1216
BspANI GGCC 1 cut(s) 84
BspCNI CTCAG 1 cut(s) 156
BspFNI CGCG 1 cut(s) 1047
BspLI GGNNCC 4 cut(s) 453, 792, 810, 916
BspPI GGATC 4 cut(s) 159, 284, 629, 869
BspQI GCTCTTC 1 cut(s) 1140
BsrDI GCAATG 2 cut(s) 507, 533
BsrFI RCCGGY 1 cut(s) 105
BsrGI TGTACA 1 cut(s) 1192
BsrI ACTGG 4 cut(s) 256, 679, 1003, 1250
BssAI RCCGGY 1 cut(s) 105
BssECI CCNNGG 6 cut(s) 158, 804, 1058, 1085, 1315, 1316
BssMI GATC 6 cut(s) 151, 289, 634, 861, 1042, 1339
BssT1I CCWWGG 4 cut(s) 158, 804, 1058, 1085
Bst2UI CCWGG 2 cut(s) 1115, 1317
Bst4CI ACNGT 4 cut(s) 370, 377, 446, 1166
Bst6I CTCTTC 2 cut(s) 1140, 1336
BstAUI TGTACA 1 cut(s) 1192
BstDEI CTNAG 1 cut(s) 164
BstDSI CCRYGG 1 cut(s) 1058
BstF5I GGATG 3 cut(s) 154, 775, 1078
BstFNI CGCG 1 cut(s) 1047
BstHHI GCGC 1 cut(s) 1049
BstKTI GATC 6 cut(s) 154, 292, 637, 864, 1045, 1342
BstMAI GTCTC 3 cut(s) 123, 518, 743
BstMBI GATC 6 cut(s) 151, 289, 634, 861, 1042, 1339
BstMWI GCNNNNNNNGC 2 cut(s) 899, 921
BstNI CCWGG 2 cut(s) 1115, 1317
BstNSI RCATGY 2 cut(s) 1193, 1199
BstSCI CCNGG 2 cut(s) 1113, 1315
BstSFI CTRYAG 1 cut(s) 261
BstUI CGCG 1 cut(s) 1047
BstV1I GCAGC 1 cut(s) 990
BstV2I GAAGAC 2 cut(s) 392, 1032
BsuRI GGCC 1 cut(s) 84
BtgI CCRYGG 1 cut(s) 1058
BtsCI GGATG 3 cut(s) 154, 775, 1078
BtsI GCAGTG 1 cut(s) 139
BtsIMutI CAGTG 3 cut(s) 139, 249, 996
CfoI GCGC 1 cut(s) 1049
Cfr10I RCCGGY 1 cut(s) 105
Cfr13I GGNCC 3 cut(s) 83, 808, 1247
Csp6I GTAC 3 cut(s) 687, 1090, 1193
CviQI GTAC 3 cut(s) 687, 1090, 1193
DdeI CTNAG 1 cut(s) 164
DpnI GATC 6 cut(s) 153, 291, 636, 863, 1044, 1341
DpnII GATC 6 cut(s) 151, 289, 634, 861, 1042, 1339
Eam1104I CTCTTC 2 cut(s) 1140, 1336
EarI CTCTTC 2 cut(s) 1140, 1336
Eco130I CCWWGG 4 cut(s) 158, 804, 1058, 1085
Eco47I GGWCC 2 cut(s) 808, 1247
Eco57I CTGAAG 1 cut(s) 840
EcoO109I RGGNCCY 1 cut(s) 808
EcoRII CCWGG 2 cut(s) 1113, 1315
EcoT14I CCWWGG 4 cut(s) 158, 804, 1058, 1085
EcoT22I ATGCAT 5 cut(s) 553, 1015, 1080, 1130, 1201
ErhI CCWWGG 4 cut(s) 158, 804, 1058, 1085
Esp3I CGTCTC 1 cut(s) 123
FalI AAGNNNNNCTT 6 cut(s) 736, 768, 1013, 1045, 1137, 1169
FaqI GGGAC 2 cut(s) 674, 794
FauI CCCGC 1 cut(s) 110
FauNDI CATATG 1 cut(s) 979
Fnu4HI GCNGC 3 cut(s) 51, 54, 1004
FokI GGATG 3 cut(s) 141, 782, 1065
Fsp4HI GCNGC 3 cut(s) 51, 54, 1004
FspBI CTAG 5 cut(s) 294, 306, 462, 594, 1235
GlaI GCGC 1 cut(s) 1048
GluI GCNGC 3 cut(s) 51, 54, 1004
GsaI CCCAGC 1 cut(s) 769
HaeIII GGCC 1 cut(s) 84
HapII CCGG 2 cut(s) 106, 948
HhaI GCGC 1 cut(s) 1049
Hin6I GCGC 1 cut(s) 1047
HinP1I GCGC 1 cut(s) 1047
HincII GTYRAC 1 cut(s) 1171
HindII GTYRAC 1 cut(s) 1171
HindIII AAGCTT 1 cut(s) 852
HinfI GANTC 1 cut(s) 1363
HpaII CCGG 2 cut(s) 106, 948
HphI GGTGA 3 cut(s) 266, 494, 856
Hpy166II GTNNAC 5 cut(s) 373, 1171, 1247, 1255, 1300
Hpy188I TCNGA 3 cut(s) 187, 394, 883
Hpy188III TCNNGA 4 cut(s) 67, 194, 449, 731
Hpy8I GTNNAC 5 cut(s) 373, 1171, 1247, 1255, 1300
Hpy99I CGWCG 1 cut(s) 129
HpyAV CCTTC 3 cut(s) 319, 774, 821
HpyCH4III ACNGT 4 cut(s) 370, 377, 446, 1166
HpyCH4IV ACGT 1 cut(s) 177
HpyF10VI GCNNNNNNNGC 2 cut(s) 899, 921
HpyF3I CTNAG 1 cut(s) 164
HpySE526I ACGT 1 cut(s) 177
HspAI GCGC 1 cut(s) 1047
Kzo9I GATC 6 cut(s) 151, 289, 634, 861, 1042, 1339
LguI GCTCTTC 1 cut(s) 1140
LmnI GCTCC 3 cut(s) 188, 790, 920
Lsp1109I GCAGC 1 cut(s) 990
LweI GCATC 7 cut(s) 247, 429, 538, 724, 760, 934, 1087
MaeI CTAG 5 cut(s) 294, 306, 462, 594, 1235
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 5 cut(s) 42, 134, 247, 1051, 1325
MalI GATC 6 cut(s) 153, 291, 636, 863, 1044, 1341
MboI GATC 6 cut(s) 151, 289, 634, 861, 1042, 1339
MboII GAAGA 5 cut(s) 364, 392, 1037, 1157, 1323
MluCI AATT 5 cut(s) 406, 564, 651, 1354, 1372
MmeI TCCRAC 1 cut(s) 942
Mph1103I ATGCAT 5 cut(s) 553, 1015, 1080, 1130, 1201
MroXI GAANNNNTTC 1 cut(s) 1149
MseI TTAA 4 cut(s) 98, 665, 870, 1375
MslI CAYNNNNRTG 1 cut(s) 1194
MspI CCGG 2 cut(s) 106, 948
MspR9I CCNGG 2 cut(s) 1115, 1317
Mva1269I GAATGC 1 cut(s) 1130
MvaI CCWGG 2 cut(s) 1115, 1317
MvnI CGCG 1 cut(s) 1047
MwoI GCNNNNNNNGC 2 cut(s) 899, 921
NcoI CCATGG 1 cut(s) 1058
NdeI CATATG 1 cut(s) 979
NdeII GATC 6 cut(s) 151, 289, 634, 861, 1042, 1339
NlaIV GGNNCC 4 cut(s) 453, 792, 810, 916
NmuCI GTSAC 4 cut(s) 134, 247, 1051, 1325
NsiI ATGCAT 5 cut(s) 553, 1015, 1080, 1130, 1201
NspI RCATGY 2 cut(s) 1193, 1199
PasI CCCWGGG 1 cut(s) 1316
PciI ACATGT 1 cut(s) 1189
PciSI GCTCTTC 1 cut(s) 1140
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 1130
PdmI GAANNNNTTC 1 cut(s) 1149
PfeI GAWTC 1 cut(s) 1363
PflMI CCANNNNNTGG 1 cut(s) 625
PkrI GCNGC 3 cut(s) 52, 55, 1005
PpuMI RGGWCCY 1 cut(s) 808
PscI ACATGT 1 cut(s) 1189
PsiI TTATAA 1 cut(s) 650
Psp1406I AACGTT 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 808
Psp6I CCWGG 2 cut(s) 1113, 1315
PspFI CCCAGC 1 cut(s) 765
PspGI CCWGG 2 cut(s) 1113, 1315
PspN4I GGNNCC 4 cut(s) 453, 792, 810, 916
PspPI GGNCC 3 cut(s) 83, 808, 1247
PspPPI RGGWCCY 1 cut(s) 808
PsrI GAACNNNNNNTAC 2 cut(s) 1205, 1237
RsaI GTAC 3 cut(s) 688, 1091, 1194
RsaNI GTAC 3 cut(s) 687, 1090, 1193
RseI CAYNNNNRTG 1 cut(s) 1194
SapI GCTCTTC 1 cut(s) 1140
SaqAI TTAA 4 cut(s) 98, 665, 870, 1375
SatI GCNGC 3 cut(s) 51, 54, 1004
Sau3AI GATC 6 cut(s) 151, 289, 634, 861, 1042, 1339
Sau96I GGNCC 3 cut(s) 83, 808, 1247
ScrFI CCNGG 2 cut(s) 1115, 1317
SfaNI GCATC 7 cut(s) 247, 429, 538, 724, 760, 934, 1087
SfcI CTRYAG 1 cut(s) 261
SinI GGWCC 2 cut(s) 808, 1247
SmiMI CAYNNNNRTG 1 cut(s) 1194
SmlI CTYRAG 1 cut(s) 849
SmoI CTYRAG 1 cut(s) 849
SpeI ACTAGT 1 cut(s) 593
Sse9I AATT 5 cut(s) 406, 564, 651, 1354, 1372
SsiI CCGC 5 cut(s) 51, 54, 103, 284, 1216
SspMI CTAG 5 cut(s) 294, 306, 462, 594, 1235
StyD4I CCNGG 2 cut(s) 1113, 1315
StyI CCWWGG 4 cut(s) 158, 804, 1058, 1085
TaaI ACNGT 4 cut(s) 370, 377, 446, 1166
TaiI ACGT 1 cut(s) 180
TaqI TCGA 1 cut(s) 111
TasI AATT 5 cut(s) 406, 564, 651, 1354, 1372
TatI WGTACW 1 cut(s) 1192
TauI GCSGC 2 cut(s) 53, 56
TfiI GAWTC 1 cut(s) 1363
Tru1I TTAA 4 cut(s) 98, 665, 870, 1375
Tru9I TTAA 4 cut(s) 98, 665, 870, 1375
TscAI CASTG 3 cut(s) 139, 256, 1003
TseFI GTSAC 4 cut(s) 134, 247, 1051, 1325
TseI GCWGC 1 cut(s) 1003
Tsp45I GTSAC 4 cut(s) 134, 247, 1051, 1325
TspDTI ATGAA 2 cut(s) 775, 1226
TspRI CASTG 3 cut(s) 139, 256, 1003
Van91I CCANNNNNTGG 1 cut(s) 625
VpaK11BI GGWCC 2 cut(s) 808, 1247
XceI RCATGY 2 cut(s) 1193, 1199
XcmI CCANNNNNNNNNTGG 1 cut(s) 231
XmnI GAANNNNTTC 1 cut(s) 1149
XspI CTAG 5 cut(s) 294, 306, 462, 594, 1235
Zsp2I ATGCAT 5 cut(s) 553, 1015, 1080, 1130, 1201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.