Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004573

Physical Location & Seq

Genomic Coordinates Forward (+)

22917107 .. 22922719

Gene Structure 5613 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1231100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGCGAATCGATTCGCAGCTCAAGTCACTCAACAAACGAACCCAACACGATATGATCAAACTTACTCACATTCCTCATCATCCCCAGCTAGAATTTTCAACCTCCATCTTCAATGCCATGCCGAACTGCATACCATGGGTCACTCTGATCATTTTCTTGACTGTAACAGAAATGGCGGTGGCACTGACTCAGTCTCCCATTCCCACCACTCTAGAAGGCCCGTTCGTGCCTGCAACTCGGCGCTTCGATCCATCGCTTCGCAGAGGCAGTGATGACTTGCCAATGGACCATCCCAGGCTCAAGAAGAACGTGACATCAAATTTCCCAGAACAGATTGCCCTTGCTATTTCTTCACCCACTGCAATGTGGGTTTCTTGGGTTACTGGGGATGCCCAGATTGGTAAAACTGTGAGACCACTTGATCCTTCAACAGTTGGGAGTGAGGTGAGGTATGGGGAAGAAAGTGGCAAGTATACGAGTGTGAAGAAAGGGGTTTCAGTTGTTTATAGTCAATTGTACCCTTTTGCTGGCCTCTTGAATTATACCTCTGCCATCATTCATCATGTCAGGATTGATGGTCTTAAACCGGGTACGAAATATTATTACAAGTGTGGGGATAGTTCTATCCCTGCTATGAGTCAAGAGCACGCCCTTGAAACTTTACCAGAGCCCGGCGACAACACATATCCTCGTCGAATAGCTGTCGTTGGAGATTTGGGTCTCACTAGCAATACCAGTACAACCATTGATCATTTAATCCAGAATGATCCTTCAATGATTTTAATGGTTGGGGACTTATGTTATGCAAACCAGTACCGTACGACTGGTGGGAAAGGAGCTTCATGCTTTTCATGTGCATTCCCAGATGCACCTATCAGAGAAACATATCAACCCCGCTGGGATGGGTGGGGAAGGTTCATGGAGCCTTTAACCTCGAGAGTTCCTATGATGGTTATTGAGGGCAACCATGAGATTGAGCCCCAAGTTTCTGGAATCACTTTCGAATCCTATTTGACAAGATTTGCAGTGCCATCAGAGGAAGCCGGCTCTAAAAGTAACTTCTACTATTCTTTTGATGCTGGAGGAGTACATTTTATCATGTTGGGAGCATATATTGACTACAATGCTACTGGTGCTCAATATGCTTGGCTTAAAGATGATCTACATCGAGTAGACCGTACAGTGACTCCCTGGCTCGTAGCAGCATGGCACCCTCCTTGGTATAACAGCTATTCTTCTCACTATCAGGAATTTGAATGCATGAGACAAGAAATGGAAGCACTTCTTTATCAATATGGTGTTGATATTGTTTTTTCTGGTCATGTGCATGCCTACGAACGGATGAATAGAGTATATAACTATACGTTGGATCCGTGCGGGCCTCTTTACATAACAGTTGGAGATGGTGGAAATATTGAAGAAGTTGATGTTGATTTCGCTGATGACCCTGGAAAGTGTCCTTCAGCTGGAGACAACACACCGGAGTTTGGAGGGGTATGCCATTTGAACTTTTCTTCAGGGCCTGCTAAAGGCAATTTTTGTTGGAGCAAACAGCCGGAGTGGAGTGCTTTTAGAGAAAGCAGCTTTGGGCATGGAATTCTTGAGGTTGTGAATTCTACTTATGCTCTATGGACTTGGCATCGGAATCAAGATACGTATAAGGAAGATAGCCACGGTGATCAAGTATACATTGTGCGACAACCTGAACTGTGCTCACGGTCTGCAAAGCCCTTGGTTTCCCCCTCTGTGCCCTCCCTCTCTGCTGCATTGCACTCTGTGGTGCTCTCTCTATACTTGCCACTCTTTGGAATGTTTTCTCTCCTCTCCTCTTCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

612

Amino Acids

68.29

Weight (kDa)

5.64

Isoelectric Point (pI)

43.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pur_ac_phosph_N	PF16656	109 - 216	1.3e-17	Purple acid Phosphatase, N-terminal domain
Metallophos	PF00149	232 - 445	8.4e-21	Calcineurin-like phosphoesterase
Metallophos_C	PF14008	513 - 552	4.3e-11	Iron/zinc purple acid phosphatase-like protein C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004573)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13700 AT4G13700
fragaria_vesca	FvH4_5g29910 FvH4_5g29910 FvH4_5g29910 FvH4_5g29910 FvH4_5g29910 FvH4_5g29910 FvH4_5g29910 FvH4_5g29910 FvH4_5g29910 FvH4_5g29910 FvH4_5g29910 FvH4_5g29910
malus_domestica	MD13G1231100.v1.1
prunus_persica	Prupe.1G061000_v2.0.a1 Prupe.1G061000_v2.0.a1
pyrus_communis	pycom13g20390
rosa_chinensis	RchiOBHm_Chr7g0223541 RchiOBHm_Chr7g0223651
rosa_laevigata	RLG00000001956
rosa_multiflora	Rmu_sc0002224.1_g000017
rosa_roxburghii	Rroxscaffold_3G00235490 Rroxscaffold_3G00235620
rosa_rugosa	Rorug07G0218100 Rorug07G0218700
rosa_samantha	Rh7AG361300 Rh7BG352800 Rh7CG379800 Rh7DG356700
rosa_wichuraiana	Rw7G030720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1209
AccB7I	CCANNNNNTGG	1 cut(s)	1805
AccI	GTMKAC	3 cut(s)	473, 1173, 1686
AciI	CCGC	3 cut(s)	176, 895, 1377
AclWI	GGATC	5 cut(s)	242, 416, 761, 1364, 1377
AcsI	RAATTY	5 cut(s)	92, 319, 1250, 1596, 1612
AcuI	CTGAAG	2 cut(s)	1446, 1500
AdeI	CACNNNGTG	1 cut(s)	1777
AfaI	GTAC	7 cut(s)	518, 592, 739, 815, 820, 1089, 1180
AfiI	CCNNNNNNNGG	7 cut(s)	527, 671, 1338, 1466, 1487, 1529, 1805
AgsI	TTSAA	9 cut(s)	99, 112, 429, 538, 656, 774, 1256, 1418, 1507
AjnI	CCWGG	3 cut(s)	293, 1190, 1447
AjuI	GAANNNNNNNTTGG	2 cut(s)	1569, 1601
AluBI	AGCT	7 cut(s)	19, 88, 701, 839, 1230, 1466, 1584
AluI	AGCT	7 cut(s)	19, 88, 701, 839, 1230, 1466, 1584
Alw21I	GWGCWC	4 cut(s)	648, 1138, 1715, 1785
Alw26I	GTCTC	5 cut(s)	198, 406, 725, 1258, 1464
AlwI	GGATC	5 cut(s)	242, 416, 761, 1364, 1377
AlwNI	CAGNNNCTG	1 cut(s)	1523
Ama87I	CYCGRG	1 cut(s)	934
AoxI	GGCC	4 cut(s)	217, 529, 1379, 1520
ApeKI	GCWGC	4 cut(s)	16, 1202, 1581, 1763
ApoI	RAATTY	5 cut(s)	92, 319, 1250, 1596, 1612
Asp700I	GAANNNNTTC	3 cut(s)	10, 1281, 1813
AspLEI	GCGC	1 cut(s)	243
AspS9I	GGNCC	4 cut(s)	218, 286, 1379, 1520
AsuC2I	CCSGG	2 cut(s)	588, 672
AsuHPI	GGTGA	3 cut(s)	345, 457, 1688
AsuII	TTCGAA	1 cut(s)	1002
AvaI	CYCGRG	1 cut(s)	934
AvaII	GGWCC	1 cut(s)	286
BaeGI	GKGCMC	1 cut(s)	1752
BamHI	GGATCC	1 cut(s)	1369
BanI	GGYRCC	1 cut(s)	1209
BanII	GRGCYC	2 cut(s)	672, 981
Bbv12I	GWGCWC	4 cut(s)	648, 1138, 1715, 1785
BbvI	GCAGC	4 cut(s)	28, 1214, 1593, 1750
BccI	CCATC	9 cut(s)	113, 259, 297, 560, 569, 896, 943, 1039, 1397
BciT130I	CCWGG	3 cut(s)	295, 1192, 1449
BclI	TGATCA	4 cut(s)	54, 147, 748, 1678
BcnI	CCSGG	2 cut(s)	588, 672
BcoDI	GTCTC	5 cut(s)	198, 406, 725, 1258, 1464
BfaI	CTAG	4 cut(s)	89, 212, 726, 1834
BfoI	RGCGCY	1 cut(s)	244
BisI	GCNGC	4 cut(s)	17, 1203, 1582, 1764
BlsI	GCNGC	4 cut(s)	18, 1204, 1583, 1765
Bme1390I	CCNGG	5 cut(s)	295, 588, 672, 1192, 1449
Bme18I	GGWCC	1 cut(s)	286
BmeT110I	CYCGRG	1 cut(s)	934
BmgT120I	GGNCC	4 cut(s)	218, 286, 1379, 1520
BmiI	GGNNCC	3 cut(s)	924, 1211, 1371
BmrFI	CCNGG	5 cut(s)	295, 588, 672, 1192, 1449
BmrI	ACTGGG	1 cut(s)	393
BmsI	GCATC	4 cut(s)	379, 856, 1066, 1648
BmuI	ACTGGG	1 cut(s)	393
BpmI	CTGGAG	2 cut(s)	1101, 1488
Bpu14I	TTCGAA	1 cut(s)	1002
BpuEI	CTTGAG	3 cut(s)	5, 284, 1622
BpuMI	CCSGG	2 cut(s)	588, 672
Bsa29I	ATCGAT	1 cut(s)	9
BsaAI	YACGTR	1 cut(s)	1656
BsaBI	GATNNNNATC	1 cut(s)	1164
BsaI	GGTCTC	2 cut(s)	406, 725
BsaJI	CCNNGG	7 cut(s)	134, 293, 1190, 1217, 1447, 1672, 1731
BsaWI	WCCGGW	1 cut(s)	1480
BsaXI	ACNNNNNCTCC	4 cut(s)	178, 208, 1171, 1201
Bsc4I	CCNNNNNNNGG	7 cut(s)	527, 671, 1338, 1466, 1487, 1529, 1805
Bse118I	RCCGGY	1 cut(s)	1043
Bse1I	ACTGG	5 cut(s)	388, 735, 811, 829, 1135
Bse3DI	GCAATG	2 cut(s)	369, 1766
Bse8I	GATNNNNATC	1 cut(s)	1164
BseBI	CCWGG	3 cut(s)	295, 1192, 1449
BseCI	ATCGAT	1 cut(s)	9
BseDI	CCNNGG	7 cut(s)	134, 293, 1190, 1217, 1447, 1672, 1731
BseGI	GGATG	5 cut(s)	79, 289, 394, 907, 1347
BseJI	GATNNNNATC	1 cut(s)	1164
BseLI	CCNNNNNNNGG	7 cut(s)	527, 671, 1338, 1466, 1487, 1529, 1805
BseMI	GCAATG	2 cut(s)	369, 1766
BseMII	CTCAG	1 cut(s)	203
BseNI	ACTGG	5 cut(s)	388, 735, 811, 829, 1135
BseRI	GAGGAG	3 cut(s)	1098, 1811, 1816
BseSI	GKGCMC	1 cut(s)	1752
BseXI	GCAGC	4 cut(s)	28, 1214, 1593, 1750
BseYI	CCCAGC	2 cut(s)	84, 897
BshFI	GGCC	4 cut(s)	219, 531, 1381, 1522
BshNI	GGYRCC	1 cut(s)	1209
BshVI	ATCGAT	1 cut(s)	9
BsiHKAI	GWGCWC	4 cut(s)	648, 1138, 1715, 1785
BsiHKCI	CYCGRG	1 cut(s)	934
BsiSI	CCGG	5 cut(s)	587, 672, 1044, 1481, 1556
BsiWI	CGTACG	1 cut(s)	818
BslFI	GGGAC	1 cut(s)	806
BslI	CCNNNNNNNGG	7 cut(s)	527, 671, 1338, 1466, 1487, 1529, 1805
BsmAI	GTCTC	5 cut(s)	198, 406, 725, 1258, 1464
BsmFI	GGGAC	1 cut(s)	806
BsmI	GAATGC	2 cut(s)	857, 1262
BsnI	GGCC	4 cut(s)	219, 531, 1381, 1522
Bso31I	GGTCTC	2 cut(s)	406, 725
BsoBI	CYCGRG	1 cut(s)	934
Bsp119I	TTCGAA	1 cut(s)	1002
Bsp1286I	GDGCHC	7 cut(s)	648, 672, 981, 1138, 1715, 1752, 1785
Bsp143I	GATC	9 cut(s)	54, 147, 247, 421, 748, 766, 1159, 1369, 1678
Bsp19I	CCATGG	1 cut(s)	134
BspACI	CCGC	3 cut(s)	176, 895, 1377
BspANI	GGCC	4 cut(s)	219, 531, 1381, 1522
BspCNI	CTCAG	1 cut(s)	202
BspDI	ATCGAT	1 cut(s)	9
BspLI	GGNNCC	3 cut(s)	924, 1211, 1371
BspPI	GGATC	5 cut(s)	242, 416, 761, 1364, 1377
BspT104I	TTCGAA	1 cut(s)	1002
BspT107I	GGYRCC	1 cut(s)	1209
BspTNI	GGTCTC	2 cut(s)	406, 725
BsrDI	GCAATG	2 cut(s)	369, 1766
BsrFI	RCCGGY	1 cut(s)	1043
BsrI	ACTGG	5 cut(s)	388, 735, 811, 829, 1135
BssAI	RCCGGY	1 cut(s)	1043
BssECI	CCNNGG	7 cut(s)	134, 293, 1190, 1217, 1447, 1672, 1731
BssMI	GATC	9 cut(s)	54, 147, 247, 421, 748, 766, 1159, 1369, 1678
BssNAI	GTATAC	2 cut(s)	474, 1687
BssT1I	CCWWGG	3 cut(s)	134, 1217, 1731
Bst1107I	GTATAC	2 cut(s)	474, 1687
Bst2UI	CCWGG	3 cut(s)	295, 1192, 1449
BstBAI	YACGTR	1 cut(s)	1656
BstBI	TTCGAA	1 cut(s)	1002
BstC8I	GCNNGC	7 cut(s)	231, 529, 648, 1045, 1329, 1379, 1524
BstDEI	CTNAG	1 cut(s)	189
BstDSI	CCRYGG	2 cut(s)	134, 1672
BstENI	CCTNNNNNAGG	1 cut(s)	1527
BstF5I	GGATG	5 cut(s)	79, 289, 394, 907, 1347
BstH2I	RGCGCY	1 cut(s)	244
BstHHI	GCGC	1 cut(s)	243
BstKTI	GATC	9 cut(s)	57, 150, 250, 424, 751, 769, 1162, 1372, 1681
BstMAI	GTCTC	5 cut(s)	198, 406, 725, 1258, 1464
BstMBI	GATC	9 cut(s)	54, 147, 247, 421, 748, 766, 1159, 1369, 1678
BstMWI	GCNNNNNNNGC	2 cut(s)	1133, 1142
BstNI	CCWGG	3 cut(s)	295, 1192, 1449
BstNSI	RCATGY	1 cut(s)	1331
BstSCI	CCNGG	5 cut(s)	293, 586, 670, 1190, 1447
BstSLI	GKGCMC	1 cut(s)	1752
BstSNI	TACGTA	1 cut(s)	1656
BstV1I	GCAGC	4 cut(s)	28, 1214, 1593, 1750
BstX2I	RGATCY	1 cut(s)	1369
BstXI	CCANNNNNNTGG	1 cut(s)	989
BstYI	RGATCY	1 cut(s)	1369
BstZ17I	GTATAC	2 cut(s)	474, 1687
Bsu15I	ATCGAT	1 cut(s)	9
BsuRI	GGCC	4 cut(s)	219, 531, 1381, 1522
BsuTUI	ATCGAT	1 cut(s)	9
BtgI	CCRYGG	2 cut(s)	134, 1672
BtgZI	GCGATG	1 cut(s)	237
BtsCI	GGATG	5 cut(s)	79, 289, 394, 907, 1347
BtsI	GCAGTG	3 cut(s)	274, 357, 1032
BtsIMutI	CAGTG	5 cut(s)	182, 274, 357, 1032, 1188
Cac8I	GCNNGC	7 cut(s)	231, 529, 648, 1045, 1329, 1379, 1524
CaiI	CAGNNNCTG	1 cut(s)	1523
CfoI	GCGC	1 cut(s)	243
Cfr10I	RCCGGY	1 cut(s)	1043
Cfr13I	GGNCC	4 cut(s)	218, 286, 1379, 1520
ClaI	ATCGAT	1 cut(s)	9
Csp6I	GTAC	7 cut(s)	517, 591, 738, 814, 819, 1088, 1179
CviQI	GTAC	7 cut(s)	517, 591, 738, 814, 819, 1088, 1179
DdeI	CTNAG	1 cut(s)	189
DpnI	GATC	9 cut(s)	56, 149, 249, 423, 750, 768, 1161, 1371, 1680
DpnII	GATC	9 cut(s)	54, 147, 247, 421, 748, 766, 1159, 1369, 1678
DraIII	CACNNNGTG	1 cut(s)	1777
Eco105I	TACGTA	1 cut(s)	1656
Eco130I	CCWWGG	3 cut(s)	134, 1217, 1731
Eco24I	GRGCYC	2 cut(s)	672, 981
Eco31I	GGTCTC	2 cut(s)	406, 725
Eco47I	GGWCC	1 cut(s)	286
Eco57I	CTGAAG	2 cut(s)	1446, 1500
Eco88I	CYCGRG	1 cut(s)	934
EcoNI	CCTNNNNNAGG	1 cut(s)	1527
EcoO109I	RGGNCCY	1 cut(s)	1520
EcoRI	GAATTC	2 cut(s)	1596, 1612
EcoRII	CCWGG	3 cut(s)	293, 1190, 1447
EcoT14I	CCWWGG	3 cut(s)	134, 1217, 1731
EcoT22I	ATGCAT	1 cut(s)	1262
EcoT38I	GRGCYC	2 cut(s)	672, 981
ErhI	CCWWGG	3 cut(s)	134, 1217, 1731
FalI	AAGNNNNNCTT	2 cut(s)	1269, 1301
FaqI	GGGAC	1 cut(s)	806
FauI	CCCGC	2 cut(s)	902, 1370
FbaI	TGATCA	4 cut(s)	54, 147, 748, 1678
FblI	GTMKAC	3 cut(s)	473, 1173, 1686
Fnu4HI	GCNGC	4 cut(s)	17, 1203, 1582, 1764
FokI	GGATG	5 cut(s)	66, 276, 401, 914, 1354
FriOI	GRGCYC	2 cut(s)	672, 981
Fsp4HI	GCNGC	4 cut(s)	17, 1203, 1582, 1764
FspBI	CTAG	4 cut(s)	89, 212, 726, 1834
GlaI	GCGC	1 cut(s)	242
GluI	GCNGC	4 cut(s)	17, 1203, 1582, 1764
GsaI	CCCAGC	2 cut(s)	88, 901
GsuI	CTGGAG	2 cut(s)	1101, 1488
HaeII	RGCGCY	1 cut(s)	244
HaeIII	GGCC	4 cut(s)	219, 531, 1381, 1522
HapII	CCGG	5 cut(s)	587, 672, 1044, 1481, 1556
HhaI	GCGC	1 cut(s)	243
Hin6I	GCGC	1 cut(s)	241
HinP1I	GCGC	1 cut(s)	241
HinfI	GANTC	8 cut(s)	6, 11, 187, 637, 993, 1004, 1186, 1645
HpaII	CCGG	5 cut(s)	587, 672, 1044, 1481, 1556
HphI	GGTGA	3 cut(s)	345, 457, 1688
Hpy166II	GTNNAC	3 cut(s)	474, 1174, 1687
Hpy188I	TCNGA	4 cut(s)	147, 878, 1036, 1644
Hpy8I	GTNNAC	3 cut(s)	474, 1174, 1687
Hpy99I	CGWCG	1 cut(s)	696
HpyAV	CCTTC	5 cut(s)	209, 435, 780, 906, 1470
HpyCH4IV	ACGT	3 cut(s)	309, 1364, 1655
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1133, 1142
HpyF3I	CTNAG	1 cut(s)	189
HpySE526I	ACGT	3 cut(s)	309, 1364, 1655
HspAI	GCGC	1 cut(s)	241
KroI	GCCGGC	1 cut(s)	1043
KroNI	GCCGGC	1 cut(s)	1045
Ksp22I	TGATCA	4 cut(s)	54, 147, 748, 1678
Kzo9I	GATC	9 cut(s)	54, 147, 247, 421, 748, 766, 1159, 1369, 1678
LmnI	GCTCC	4 cut(s)	836, 922, 1106, 1545
Lsp1109I	GCAGC	4 cut(s)	28, 1214, 1593, 1750
LweI	GCATC	4 cut(s)	379, 856, 1066, 1648
MaeI	CTAG	4 cut(s)	89, 212, 726, 1834
MaeII	ACGT	3 cut(s)	309, 1364, 1655
MaeIII	GTNAC	7 cut(s)	24, 139, 163, 310, 379, 1055, 1183
MalI	GATC	9 cut(s)	56, 149, 249, 423, 750, 768, 1161, 1371, 1680
MboI	GATC	9 cut(s)	54, 147, 247, 421, 748, 766, 1159, 1369, 1678
MfeI	CAATTG	1 cut(s)	512
MflI	RGATCY	1 cut(s)	1369
MhlI	GDGCHC	7 cut(s)	648, 672, 981, 1138, 1715, 1752, 1785
MluCI	AATT	8 cut(s)	92, 319, 512, 538, 1250, 1534, 1596, 1612
MlyI	GAGTC	3 cut(s)	181, 646, 1180
MmeI	TCCRAC	4 cut(s)	688, 1347, 1378, 1523
Mph1103I	ATGCAT	1 cut(s)	1262
MroNI	GCCGGC	1 cut(s)	1043
MroXI	GAANNNNTTC	3 cut(s)	10, 1281, 1813
MseI	TTAA	5 cut(s)	582, 755, 782, 929, 1152
MslI	CAYNNNNRTG	2 cut(s)	362, 1326
MspA1I	CMGCKG	2 cut(s)	897, 1466
MspI	CCGG	5 cut(s)	587, 672, 1044, 1481, 1556
MspR9I	CCNGG	5 cut(s)	295, 588, 672, 1192, 1449
MunI	CAATTG	1 cut(s)	512
Mva1269I	GAATGC	2 cut(s)	857, 1262
MvaI	CCWGG	3 cut(s)	295, 1192, 1449
MwoI	GCNNNNNNNGC	2 cut(s)	1133, 1142
NaeI	GCCGGC	1 cut(s)	1045
NciI	CCSGG	2 cut(s)	588, 672
NcoI	CCATGG	1 cut(s)	134
NdeII	GATC	9 cut(s)	54, 147, 247, 421, 748, 766, 1159, 1369, 1678
NgoMIV	GCCGGC	1 cut(s)	1043
NlaIV	GGNNCC	3 cut(s)	924, 1211, 1371
NmeAIII	GCCGAG	1 cut(s)	217
NmuCI	GTSAC	4 cut(s)	24, 139, 310, 1183
NsiI	ATGCAT	1 cut(s)	1262
NspI	RCATGY	1 cut(s)	1331
NspV	TTCGAA	1 cut(s)	1002
PaeI	GCATGC	1 cut(s)	1331
PaeR7I	CTCGAG	1 cut(s)	934
PcsI	WCGNNNNNNNCGW	2 cut(s)	1174, 1370
PctI	GAATGC	2 cut(s)	857, 1262
PdiI	GCCGGC	1 cut(s)	1045
PdmI	GAANNNNTTC	3 cut(s)	10, 1281, 1813
PfeI	GAWTC	5 cut(s)	6, 11, 993, 1004, 1645
Pfl23II	CGTACG	1 cut(s)	818
PflFI	GACNNNGTC	1 cut(s)	190
PflMI	CCANNNNNTGG	1 cut(s)	1805
PkrI	GCNGC	4 cut(s)	18, 1204, 1583, 1765
PleI	GAGTC	3 cut(s)	181, 645, 1180
PpsI	GAGTC	3 cut(s)	181, 645, 1180
Ppu21I	YACGTR	1 cut(s)	1656
Psp6I	CCWGG	3 cut(s)	293, 1190, 1447
PspFI	CCCAGC	2 cut(s)	84, 897
PspGI	CCWGG	3 cut(s)	293, 1190, 1447
PspLI	CGTACG	1 cut(s)	818
PspN4I	GGNNCC	3 cut(s)	924, 1211, 1371
PspPI	GGNCC	4 cut(s)	218, 286, 1379, 1520
PstNI	CAGNNNCTG	1 cut(s)	1523
PsuI	RGATCY	1 cut(s)	1369
PsyI	GACNNNGTC	1 cut(s)	190
PvuII	CAGCTG	1 cut(s)	1466
RsaI	GTAC	7 cut(s)	518, 592, 739, 815, 820, 1089, 1180
RsaNI	GTAC	7 cut(s)	517, 591, 738, 814, 819, 1088, 1179
RseI	CAYNNNNRTG	2 cut(s)	362, 1326
SaqAI	TTAA	5 cut(s)	582, 755, 782, 929, 1152
SatI	GCNGC	4 cut(s)	17, 1203, 1582, 1764
Sau3AI	GATC	9 cut(s)	54, 147, 247, 421, 748, 766, 1159, 1369, 1678
Sau96I	GGNCC	4 cut(s)	218, 286, 1379, 1520
SchI	GAGTC	3 cut(s)	181, 646, 1180
ScrFI	CCNGG	5 cut(s)	295, 588, 672, 1192, 1449
SduI	GDGCHC	7 cut(s)	648, 672, 981, 1138, 1715, 1752, 1785
SfaNI	GCATC	4 cut(s)	379, 856, 1066, 1648
Sfr274I	CTCGAG	1 cut(s)	934
SfuI	TTCGAA	1 cut(s)	1002
SinI	GGWCC	1 cut(s)	286
SlaI	CTCGAG	1 cut(s)	934
SmiMI	CAYNNNNRTG	2 cut(s)	362, 1326
SmlI	CTYRAG	4 cut(s)	20, 299, 934, 1601
SmoI	CTYRAG	4 cut(s)	20, 299, 934, 1601
SnaBI	TACGTA	1 cut(s)	1656
SphI	GCATGC	1 cut(s)	1331
Sse9I	AATT	8 cut(s)	92, 319, 512, 538, 1250, 1534, 1596, 1612
SsiI	CCGC	3 cut(s)	176, 895, 1377
SspI	AATATT	2 cut(s)	599, 1414
SspMI	CTAG	4 cut(s)	89, 212, 726, 1834
StyD4I	CCNGG	5 cut(s)	293, 586, 670, 1190, 1447
StyI	CCWWGG	3 cut(s)	134, 1217, 1731
TaiI	ACGT	3 cut(s)	312, 1367, 1658
TaqI	TCGA	6 cut(s)	9, 246, 694, 935, 1002, 1168
TasI	AATT	8 cut(s)	92, 319, 512, 538, 1250, 1534, 1596, 1612
TatI	WGTACW	2 cut(s)	737, 1087
TfiI	GAWTC	5 cut(s)	6, 11, 993, 1004, 1645
Tru1I	TTAA	5 cut(s)	582, 755, 782, 929, 1152
Tru9I	TTAA	5 cut(s)	582, 755, 782, 929, 1152
TscAI	CASTG	5 cut(s)	189, 274, 364, 1032, 1188
TseFI	GTSAC	4 cut(s)	24, 139, 310, 1183
TseI	GCWGC	4 cut(s)	16, 1202, 1581, 1763
Tsp45I	GTSAC	4 cut(s)	24, 139, 310, 1183
TspDTI	ATGAA	5 cut(s)	548, 831, 840, 907, 1358
TspGWI	ACGGA	2 cut(s)	1354, 1362
TspRI	CASTG	5 cut(s)	189, 274, 364, 1032, 1188
Tth111I	GACNNNGTC	1 cut(s)	190
Van91I	CCANNNNNTGG	1 cut(s)	1805
VpaK11BI	GGWCC	1 cut(s)	286
XagI	CCTNNNNNAGG	1 cut(s)	1527
XapI	RAATTY	5 cut(s)	92, 319, 1250, 1596, 1612
XbaI	TCTAGA	1 cut(s)	211
XceI	RCATGY	1 cut(s)	1331
XhoI	CTCGAG	1 cut(s)	934
XmiI	GTMKAC	3 cut(s)	473, 1173, 1686
XmnI	GAANNNNTTC	3 cut(s)	10, 1281, 1813
XspI	CTAG	4 cut(s)	89, 212, 726, 1834
Zsp2I	ATGCAT	1 cut(s)	1262

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.