pycom13g20390

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004573
Physical Location & Seq
Forward (+)
16456031 .. 16458776
2746 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g20390.3

Sequence Viewer

Length: 978 bp
ATGATGGTTATTGAGGGCAACCATGAGATTGAGCCCCAAGCTTCTGGAATCACTTTCGAATCCTATTTGACTAGATTTGCAGTGCCATCAGATGAATCCGGCTCTAAAAGTAACTTCTACTATTCTTTTGATGCTGGAGGAGTACATTTTATCATGTTGGGAGCATATATTGACTACAATGCTACTGGTGCTCAATATGCTTGGCTTAAAGATGATCTACATCGAGTAGACCGTACAGTGACTCCCTGGCTCGTGGCAGCATGGCACCCTCCTTGGTATAACAGCTATTCTTCTCACTATCAGGAATTTGAATGCATGAGACAAGAAATGGAAGCACTTCTTTATCAATACGGTGTTGATATTGTGTTTTCTGGTCATGTGCATGCCTACGAACGGATGAATAGAGTATATAACTATACGTTGGATCCGTGCGGGCCTCTTTACATAACAGTTGGAGATGGTGGAAATATTGAAGAAGTTGATGTTGATTTCGCTGATGACCCTGGAAAGTGTCCTTCAGCTGGAGACAACACACCGGAGTTTGGAGGGGTATGCCATTTGAACTTTTCTTCAGGGCCTGCTAAAGGCAACTTTTGTTGGAGCAAACAGCCGGAGTGGAGTGCTTTTAGAGAAAGCAGCTTTGGGCATGGAATTCTTGAGGTGGTGAATTCTACTTATGCTCTATGGACTTGGCATCGGAATCAAGATATGTATAAGGAAGATAGCCATGGTGATCAAGTATACGTTGTGCGACAACCTGAACTGTGCTCACGGTCTGCAAAGCCCTTGGTTACCCCCCCCTCTGTGCCCTCCCTCTCTGCTGCATTGCACTCTCTGGTGCTCTCTCTATCCTTGCCACTCTTTGGAATTGATACTGCTGGTGGAGGAGGGAGGGGATTGGTATTGAAGACAATTAAAAATCGGCTGCGGAAATCAAACACAAGACTAGACCTCAAATGCAAAGAAGAAGGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

326

Amino Acids

36.35

Weight (kDa)

5.34

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 264
AccB7I CCANNNNNTGG 1 cut(s) 863
AccI GTMKAC 2 cut(s) 228, 741
AciI CCGC 2 cut(s) 432, 928
AclWI GGATC 2 cut(s) 419, 432
AcsI RAATTY 3 cut(s) 305, 651, 667
AcuI CTGAAG 2 cut(s) 501, 555
AfaI GTAC 2 cut(s) 144, 235
AfiI CCNNNNNNNGG 5 cut(s) 393, 521, 542, 584, 863
AgsI TTSAA 4 cut(s) 311, 473, 562, 907
AjnI CCWGG 2 cut(s) 245, 502
AjuI GAANNNNNNNTTGG 2 cut(s) 624, 656
AluBI AGCT 4 cut(s) 41, 285, 521, 639
AluI AGCT 4 cut(s) 41, 285, 521, 639
Alw21I GWGCWC 3 cut(s) 193, 770, 843
Alw26I GTCTC 2 cut(s) 313, 519
AlwI GGATC 2 cut(s) 419, 432
AlwNI CAGNNNCTG 1 cut(s) 578
AoxI GGCC 2 cut(s) 434, 575
ApeKI GCWGC 4 cut(s) 257, 636, 821, 925
ApoI RAATTY 3 cut(s) 305, 651, 667
Asp700I GAANNNNTTC 1 cut(s) 336
AspS9I GGNCC 2 cut(s) 434, 575
AsuHPI GGTGA 2 cut(s) 676, 743
AsuII TTCGAA 1 cut(s) 57
BaeGI GKGCMC 1 cut(s) 810
BamHI GGATCC 1 cut(s) 424
BanI GGYRCC 1 cut(s) 264
BanII GRGCYC 1 cut(s) 36
BauI CACGAG 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 914
Bbv12I GWGCWC 3 cut(s) 193, 770, 843
BbvI GCAGC 4 cut(s) 269, 648, 808, 912
BccI CCATC 2 cut(s) 94, 452
BciT130I CCWGG 2 cut(s) 247, 504
BclI TGATCA 1 cut(s) 733
BcoDI GTCTC 2 cut(s) 313, 519
BfaI CTAG 2 cut(s) 72, 947
BisI GCNGC 4 cut(s) 258, 637, 822, 926
BlsI GCNGC 4 cut(s) 259, 638, 823, 927
Bme1390I CCNGG 2 cut(s) 247, 504
BmgT120I GGNCC 2 cut(s) 434, 575
BmiI GGNNCC 2 cut(s) 266, 426
BmrFI CCNGG 2 cut(s) 247, 504
BmsI GCATC 2 cut(s) 121, 703
BpiI GAAGAC 1 cut(s) 914
BpmI CTGGAG 2 cut(s) 156, 543
Bpu14I TTCGAA 1 cut(s) 57
BpuEI CTTGAG 1 cut(s) 677
BsaBI GATNNNNATC 1 cut(s) 219
BsaJI CCNNGG 5 cut(s) 245, 272, 502, 727, 786
BsaWI WCCGGW 1 cut(s) 535
BsaXI ACNNNNNCTCC 2 cut(s) 226, 256
Bsc4I CCNNNNNNNGG 5 cut(s) 393, 521, 542, 584, 863
Bse1I ACTGG 1 cut(s) 190
Bse3DI GCAATG 1 cut(s) 824
Bse8I GATNNNNATC 1 cut(s) 219
BseBI CCWGG 2 cut(s) 247, 504
BseDI CCNNGG 5 cut(s) 245, 272, 502, 727, 786
BseGI GGATG 1 cut(s) 402
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 5 cut(s) 393, 521, 542, 584, 863
BseMI GCAATG 1 cut(s) 824
BseNI ACTGG 1 cut(s) 190
BseRI GAGGAG 2 cut(s) 153, 900
BseSI GKGCMC 1 cut(s) 810
BseXI GCAGC 4 cut(s) 269, 648, 808, 912
BshFI GGCC 2 cut(s) 436, 577
BshNI GGYRCC 1 cut(s) 264
BsiHKAI GWGCWC 3 cut(s) 193, 770, 843
BsiSI CCGG 3 cut(s) 99, 536, 611
BslI CCNNNNNNNGG 5 cut(s) 393, 521, 542, 584, 863
BsmAI GTCTC 2 cut(s) 313, 519
BsmI GAATGC 1 cut(s) 317
BsnI GGCC 2 cut(s) 436, 577
Bsp119I TTCGAA 1 cut(s) 57
Bsp1286I GDGCHC 5 cut(s) 36, 193, 770, 810, 843
Bsp143I GATC 3 cut(s) 214, 424, 733
Bsp19I CCATGG 1 cut(s) 727
BspACI CCGC 2 cut(s) 432, 928
BspANI GGCC 2 cut(s) 436, 577
BspLI GGNNCC 2 cut(s) 266, 426
BspPI GGATC 2 cut(s) 419, 432
BspT104I TTCGAA 1 cut(s) 57
BspT107I GGYRCC 1 cut(s) 264
BsrDI GCAATG 1 cut(s) 824
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 5 cut(s) 245, 272, 502, 727, 786
BssMI GATC 3 cut(s) 214, 424, 733
BssNAI GTATAC 1 cut(s) 742
BssSI CACGAG 1 cut(s) 251
BssT1I CCWWGG 3 cut(s) 272, 727, 786
Bst1107I GTATAC 1 cut(s) 742
Bst2BI CACGAG 1 cut(s) 251
Bst2UI CCWGG 2 cut(s) 247, 504
Bst4CI ACNGT 6 cut(s) 233, 238, 353, 451, 765, 774
BstBI TTCGAA 1 cut(s) 57
BstC8I GCNNGC 3 cut(s) 384, 434, 579
BstDSI CCRYGG 1 cut(s) 727
BstEII GGTNACC 1 cut(s) 790
BstENI CCTNNNNNAGG 1 cut(s) 582
BstF5I GGATG 1 cut(s) 402
BstKTI GATC 3 cut(s) 217, 427, 736
BstMAI GTCTC 2 cut(s) 313, 519
BstMBI GATC 3 cut(s) 214, 424, 733
BstMWI GCNNNNNNNGC 2 cut(s) 188, 197
BstNI CCWGG 2 cut(s) 247, 504
BstNSI RCATGY 1 cut(s) 386
BstPI GGTNACC 1 cut(s) 790
BstSCI CCNGG 2 cut(s) 245, 502
BstSLI GKGCMC 1 cut(s) 810
BstV1I GCAGC 4 cut(s) 269, 648, 808, 912
BstV2I GAAGAC 1 cut(s) 914
BstX2I RGATCY 1 cut(s) 424
BstXI CCANNNNNNTGG 1 cut(s) 44
BstYI RGATCY 1 cut(s) 424
BstZ17I GTATAC 1 cut(s) 742
BsuRI GGCC 2 cut(s) 436, 577
BtgI CCRYGG 1 cut(s) 727
BtsCI GGATG 1 cut(s) 402
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 2 cut(s) 87, 243
Cac8I GCNNGC 3 cut(s) 384, 434, 579
CaiI CAGNNNCTG 1 cut(s) 578
Cfr13I GGNCC 2 cut(s) 434, 575
Csp6I GTAC 2 cut(s) 143, 234
CviAII CATG 8 cut(s) 23, 154, 261, 316, 377, 383, 647, 728
CviQI GTAC 2 cut(s) 143, 234
DpnI GATC 3 cut(s) 216, 426, 735
DpnII GATC 3 cut(s) 214, 424, 733
Eco130I CCWWGG 3 cut(s) 272, 727, 786
Eco24I GRGCYC 1 cut(s) 36
Eco57I CTGAAG 2 cut(s) 501, 555
Eco91I GGTNACC 1 cut(s) 790
EcoNI CCTNNNNNAGG 1 cut(s) 582
EcoO109I RGGNCCY 1 cut(s) 575
EcoO65I GGTNACC 1 cut(s) 790
EcoRI GAATTC 2 cut(s) 651, 667
EcoRII CCWGG 2 cut(s) 245, 502
EcoT14I CCWWGG 3 cut(s) 272, 727, 786
EcoT22I ATGCAT 1 cut(s) 317
EcoT38I GRGCYC 1 cut(s) 36
ErhI CCWWGG 3 cut(s) 272, 727, 786
FaeI CATG 8 cut(s) 26, 157, 264, 319, 380, 386, 650, 731
FalI AAGNNNNNCTT 2 cut(s) 324, 356
FatI CATG 8 cut(s) 22, 153, 260, 315, 376, 382, 646, 727
FauI CCCGC 1 cut(s) 425
FbaI TGATCA 1 cut(s) 733
FblI GTMKAC 2 cut(s) 228, 741
Fnu4HI GCNGC 4 cut(s) 258, 637, 822, 926
FokI GGATG 1 cut(s) 409
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 4 cut(s) 258, 637, 822, 926
FspBI CTAG 2 cut(s) 72, 947
GluI GCNGC 4 cut(s) 258, 637, 822, 926
GsuI CTGGAG 2 cut(s) 156, 543
HaeIII GGCC 2 cut(s) 436, 577
HapII CCGG 3 cut(s) 99, 536, 611
Hin1II CATG 8 cut(s) 26, 157, 264, 319, 380, 386, 650, 731
HindIII AAGCTT 1 cut(s) 39
HinfI GANTC 5 cut(s) 48, 59, 95, 241, 700
HpaII CCGG 3 cut(s) 99, 536, 611
HphI GGTGA 2 cut(s) 676, 743
Hpy166II GTNNAC 2 cut(s) 229, 742
Hpy188I TCNGA 2 cut(s) 91, 699
Hpy188III TCNNGA 5 cut(s) 45, 302, 656, 704, 975
Hpy8I GTNNAC 2 cut(s) 229, 742
HpyAV CCTTC 2 cut(s) 525, 962
HpyCH4III ACNGT 6 cut(s) 233, 238, 353, 451, 765, 774
HpyCH4IV ACGT 2 cut(s) 419, 744
HpyCH4V TGCA 7 cut(s) 80, 315, 382, 779, 824, 829, 960
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 197
HpySE526I ACGT 2 cut(s) 419, 744
Hsp92II CATG 8 cut(s) 26, 157, 264, 319, 380, 386, 650, 731
Ksp22I TGATCA 1 cut(s) 733
Kzo9I GATC 3 cut(s) 214, 424, 733
LmnI GCTCC 2 cut(s) 161, 600
Lsp1109I GCAGC 4 cut(s) 269, 648, 808, 912
LweI GCATC 2 cut(s) 121, 703
MaeI CTAG 2 cut(s) 72, 947
MaeII ACGT 2 cut(s) 419, 744
MaeIII GTNAC 3 cut(s) 110, 238, 790
MalI GATC 3 cut(s) 216, 426, 735
MboI GATC 3 cut(s) 214, 424, 733
MboII GAAGA 6 cut(s) 282, 485, 561, 731, 919, 977
MflI RGATCY 1 cut(s) 424
MhlI GDGCHC 5 cut(s) 36, 193, 770, 810, 843
MluCI AATT 5 cut(s) 305, 651, 667, 867, 912
MlyI GAGTC 1 cut(s) 235
MmeI TCCRAC 3 cut(s) 402, 433, 578
Mph1103I ATGCAT 1 cut(s) 317
MroXI GAANNNNTTC 1 cut(s) 336
MseI TTAA 2 cut(s) 207, 915
MslI CAYNNNNRTG 1 cut(s) 381
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 3 cut(s) 99, 536, 611
MspR9I CCNGG 2 cut(s) 247, 504
Mva1269I GAATGC 1 cut(s) 317
MvaI CCWGG 2 cut(s) 247, 504
MwoI GCNNNNNNNGC 2 cut(s) 188, 197
NcoI CCATGG 1 cut(s) 727
NdeII GATC 3 cut(s) 214, 424, 733
NlaIII CATG 8 cut(s) 26, 157, 264, 319, 380, 386, 650, 731
NlaIV GGNNCC 2 cut(s) 266, 426
NmuCI GTSAC 1 cut(s) 238
NsiI ATGCAT 1 cut(s) 317
NspI RCATGY 1 cut(s) 386
NspV TTCGAA 1 cut(s) 57
PaeI GCATGC 1 cut(s) 386
PcsI WCGNNNNNNNCGW 2 cut(s) 229, 425
PctI GAATGC 1 cut(s) 317
PdmI GAANNNNTTC 1 cut(s) 336
PfeI GAWTC 4 cut(s) 48, 59, 95, 700
PflMI CCANNNNNTGG 1 cut(s) 863
PkrI GCNGC 4 cut(s) 259, 638, 823, 927
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
Psp6I CCWGG 2 cut(s) 245, 502
PspEI GGTNACC 1 cut(s) 790
PspGI CCWGG 2 cut(s) 245, 502
PspN4I GGNNCC 2 cut(s) 266, 426
PspPI GGNCC 2 cut(s) 434, 575
PstNI CAGNNNCTG 1 cut(s) 578
PsuI RGATCY 1 cut(s) 424
PvuII CAGCTG 1 cut(s) 521
RsaI GTAC 2 cut(s) 144, 235
RsaNI GTAC 2 cut(s) 143, 234
RseI CAYNNNNRTG 1 cut(s) 381
SaqAI TTAA 2 cut(s) 207, 915
SatI GCNGC 4 cut(s) 258, 637, 822, 926
Sau3AI GATC 3 cut(s) 214, 424, 733
Sau96I GGNCC 2 cut(s) 434, 575
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 2 cut(s) 247, 504
SduI GDGCHC 5 cut(s) 36, 193, 770, 810, 843
SetI ASST 9 cut(s) 43, 287, 422, 523, 641, 663, 747, 760, 954
SfaNI GCATC 2 cut(s) 121, 703
SfuI TTCGAA 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 381
SmlI CTYRAG 1 cut(s) 656
SmoI CTYRAG 1 cut(s) 656
SphI GCATGC 1 cut(s) 386
Sse9I AATT 5 cut(s) 305, 651, 667, 867, 912
SsiI CCGC 2 cut(s) 432, 928
SspI AATATT 1 cut(s) 469
SspMI CTAG 2 cut(s) 72, 947
StyD4I CCNGG 2 cut(s) 245, 502
StyI CCWWGG 3 cut(s) 272, 727, 786
TaaI ACNGT 6 cut(s) 233, 238, 353, 451, 765, 774
TaiI ACGT 2 cut(s) 422, 747
TaqI TCGA 2 cut(s) 57, 223
TasI AATT 5 cut(s) 305, 651, 667, 867, 912
TatI WGTACW 1 cut(s) 142
TfiI GAWTC 4 cut(s) 48, 59, 95, 700
Tru1I TTAA 2 cut(s) 207, 915
Tru9I TTAA 2 cut(s) 207, 915
TscAI CASTG 2 cut(s) 87, 243
TseFI GTSAC 1 cut(s) 238
TseI GCWGC 4 cut(s) 257, 636, 821, 925
Tsp45I GTSAC 1 cut(s) 238
TspDTI ATGAA 2 cut(s) 108, 413
TspGWI ACGGA 2 cut(s) 409, 417
TspRI CASTG 2 cut(s) 87, 243
Van91I CCANNNNNTGG 1 cut(s) 863
XagI CCTNNNNNAGG 1 cut(s) 582
XapI RAATTY 3 cut(s) 305, 651, 667
XceI RCATGY 1 cut(s) 386
XmiI GTMKAC 2 cut(s) 228, 741
XmnI GAANNNNTTC 1 cut(s) 336
XspI CTAG 2 cut(s) 72, 947
Zsp2I ATGCAT 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.