AT4G14010

ralf-like 32

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015943
Physical Location & Seq
Reverse (-)
8092687 .. 8093552
866 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G14010.1

Sequence Viewer

Length: 354 bp
ATGGAGATAAAACCCTCTAGGATATTCTCCACCATCACCATCTTCTTTCTCTGCCTTCTACTAGCACACGTCACCTCCAAGGCATCATCATCATCTCTGTGCAATGGATCTGTGGCGGAGTGCAGCAGCATGGTGGAGACAGAGGAAATGAGTGTGATAATGGAGTCTTGGTCAAGCCAGAGGTTGACGGAGGAGCAGGCACATAAGCTTTCGTATGGAGCTTTGAGGAGGAATCAGCCTGCTTGCGATGGTGGTAAACGAGGCGAGTCTTACTCAACTCAATGTCTTCCTCCACCGTCTAACCCTTATAGTAGAGGCTGCTCTAAGCATTACCGATGCGGCCGTGACTCTTGA

Protein Analysis

117

Amino Acids

12.92

Weight (kDa)

8.19

Isoelectric Point (pI)

71.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 51 - 115 9.2e-24 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015943)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14010
fragaria_vesca FvH4_4g13190
malus_domestica MD04G1018100.v1.1
prunus_persica Prupe.1G167100_v2.0.a1
pyrus_communis pycom13g29140
rosa_chinensis RchiOBHm_Chr4g0413341
rosa_multiflora Rmu_sc0007671.1_g000002 Rmu_sc0009573.1_g000009
rosa_roxburghii Rroxscaffold_5G00357260
rosa_rugosa Rorug04G0120000
rosa_samantha Rh4AG179400 Rh4BG178500 Rh4CG190000 Rh4DG174500
rosa_wichuraiana Rw4G014910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 116, 339
AclWI GGATC 1 cut(s) 115
AcoI YGGCCR 1 cut(s) 340
AflIII ACRYGT 1 cut(s) 67
AjiI CACGTC 1 cut(s) 70
AluBI AGCT 2 cut(s) 208, 221
AluI AGCT 2 cut(s) 208, 221
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 1 cut(s) 115
AoxI GGCC 1 cut(s) 340
ApeKI GCWGC 3 cut(s) 123, 126, 318
AsuHPI GGTGA 2 cut(s) 28, 64
BbsI GAAGAC 1 cut(s) 278
BbvI GCAGC 3 cut(s) 135, 138, 305
BccI CCATC 3 cut(s) 41, 47, 242
BceAI ACGGC 1 cut(s) 327
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 2 cut(s) 18, 62
BisI GCNGC 4 cut(s) 124, 127, 319, 340
BlsI GCNGC 4 cut(s) 125, 128, 320, 341
BmgBI CACGTC 1 cut(s) 70
BmsI GCATC 2 cut(s) 92, 326
BpiI GAAGAC 1 cut(s) 278
BplI GAGNNNNNCTC 2 cut(s) 257, 289
BsaJI CCNNGG 1 cut(s) 78
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 155, 185
Bse3DI GCAATG 1 cut(s) 109
BseDI CCNNGG 1 cut(s) 78
BseMI GCAATG 1 cut(s) 109
BseRI GAGGAG 2 cut(s) 206, 241
BseX3I CGGCCG 1 cut(s) 340
BseXI GCAGC 3 cut(s) 135, 138, 305
BsgI GTGCAG 1 cut(s) 142
Bsh1285I CGRYCG 1 cut(s) 343
BshFI GGCC 1 cut(s) 342
BsiEI CGRYCG 1 cut(s) 343
BsmAI GTCTC 1 cut(s) 131
BsnI GGCC 1 cut(s) 342
Bsp143I GATC 1 cut(s) 107
BspACI CCGC 2 cut(s) 116, 339
BspANI GGCC 1 cut(s) 342
BspPI GGATC 1 cut(s) 115
BsrDI GCAATG 1 cut(s) 109
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 1 cut(s) 107
BssT1I CCWWGG 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 297
BstC8I GCNNGC 3 cut(s) 198, 240, 244
BstDEI CTNAG 1 cut(s) 324
BstKTI GATC 1 cut(s) 110
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 1 cut(s) 107
BstMCI CGRYCG 1 cut(s) 343
BstV1I GCAGC 3 cut(s) 135, 138, 305
BstV2I GAAGAC 1 cut(s) 278
BstX2I RGATCY 1 cut(s) 107
BstYI RGATCY 1 cut(s) 107
BstZI CGGCCG 1 cut(s) 340
BsuRI GGCC 1 cut(s) 342
BtgZI GCGATG 1 cut(s) 261
BtrI CACGTC 1 cut(s) 70
Cac8I GCNNGC 3 cut(s) 198, 240, 244
CviAII CATG 1 cut(s) 130
CviJI RGCY 6 cut(s) 177, 208, 221, 238, 318, 342
CviKI_1 RGCY 6 cut(s) 177, 208, 221, 238, 318, 342
DdeI CTNAG 1 cut(s) 324
DpnI GATC 1 cut(s) 109
DpnII GATC 1 cut(s) 107
EaeI YGGCCR 1 cut(s) 340
EagI CGGCCG 1 cut(s) 340
EciI GGCGGA 1 cut(s) 131
EclXI CGGCCG 1 cut(s) 340
Eco130I CCWWGG 1 cut(s) 78
Eco52I CGGCCG 1 cut(s) 340
EcoT14I CCWWGG 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 78
FaeI CATG 1 cut(s) 133
FaiI YATR 4 cut(s) 131, 204, 216, 309
FatI CATG 1 cut(s) 129
Fnu4HI GCNGC 4 cut(s) 124, 127, 319, 340
Fsp4HI GCNGC 4 cut(s) 124, 127, 319, 340
FspBI CTAG 2 cut(s) 18, 62
GluI GCNGC 4 cut(s) 124, 127, 319, 340
HaeIII GGCC 1 cut(s) 342
Hin1II CATG 1 cut(s) 133
HincII GTYRAC 1 cut(s) 186
HindII GTYRAC 1 cut(s) 186
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 4 cut(s) 164, 232, 266, 347
HphI GGTGA 2 cut(s) 28, 64
Hpy166II GTNNAC 2 cut(s) 186, 257
Hpy188III TCNNGA 1 cut(s) 351
Hpy8I GTNNAC 2 cut(s) 186, 257
HpyAV CCTTC 1 cut(s) 65
HpyCH4III ACNGT 1 cut(s) 297
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 2 cut(s) 102, 123
HpyF3I CTNAG 1 cut(s) 324
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 1 cut(s) 133
Kzo9I GATC 1 cut(s) 107
LmnI GCTCC 2 cut(s) 193, 218
LpnPI CCDG 3 cut(s) 182, 191, 252
Lsp1109I GCAGC 3 cut(s) 135, 138, 305
LweI GCATC 2 cut(s) 92, 326
MaeI CTAG 2 cut(s) 18, 62
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 2 cut(s) 70, 344
MalI GATC 1 cut(s) 109
MboI GATC 1 cut(s) 107
MboII GAAGA 2 cut(s) 34, 278
MflI RGATCY 1 cut(s) 107
MlyI GAGTC 3 cut(s) 173, 275, 341
MslI CAYNNNNRTG 1 cut(s) 97
NdeII GATC 1 cut(s) 107
NlaIII CATG 1 cut(s) 133
NmuCI GTSAC 2 cut(s) 70, 344
PfeI GAWTC 1 cut(s) 232
PkrI GCNGC 4 cut(s) 125, 128, 320, 341
PleI GAGTC 3 cut(s) 172, 274, 341
PpsI GAGTC 3 cut(s) 172, 274, 341
PsuI RGATCY 1 cut(s) 107
RseI CAYNNNNRTG 1 cut(s) 97
SatI GCNGC 4 cut(s) 124, 127, 319, 340
Sau3AI GATC 1 cut(s) 107
SchI GAGTC 3 cut(s) 173, 275, 341
SetI ASST 5 cut(s) 72, 77, 185, 210, 223
SfaNI GCATC 2 cut(s) 92, 326
SmiMI CAYNNNNRTG 1 cut(s) 97
SsiI CCGC 2 cut(s) 116, 339
SspMI CTAG 2 cut(s) 18, 62
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 297
TaiI ACGT 1 cut(s) 72
TauI GCSGC 1 cut(s) 342
TfiI GAWTC 1 cut(s) 232
TseFI GTSAC 2 cut(s) 70, 344
TseI GCWGC 3 cut(s) 123, 126, 318
Tsp45I GTSAC 2 cut(s) 70, 344
TspGWI ACGGA 1 cut(s) 203
XspI CTAG 2 cut(s) 18, 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.