MD04G1018100.v1.1

ralf-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015943
Physical Location & Seq
Reverse (-)
2085155 .. 2085493
339 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1018100.v1.1.491

Sequence Viewer

Length: 339 bp
ATGCAAAGGCCTAACAGAGTGTTCCTTCTGCTAATCACCGGCCTTTGCTACCTCGCCTGCACAACTGCCTCGGGAACGAGCCCTACGTCCACATCGTGCAACGGTTCGATAGCGGAGTGCGACAACGAAAGCGAGCTGCTGATGGAGTCGGAGATAAGCCGGAGGTTTCTTCAGACGAAGAAGTACATATCGCCCGGGGCTTTGAAGCCAGACCAACCAGTTTGCAAAGGCGGCGCTAGAGGTGAAGCGTATAGTAAAACAGGAGGCTGTCTTCCTCCTCCGTCTCACCCTTATCAGAGAGGTTGTTCTAAGTATTATCGTTGTAGGTCGGACTCGTGA

Protein Analysis

113

Amino Acids

12.19

Weight (kDa)

8.83

Isoelectric Point (pI)

61.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 45 - 109 2.3e-24 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015943)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14010
fragaria_vesca FvH4_4g13190
malus_domestica MD04G1018100.v1.1
prunus_persica Prupe.1G167100_v2.0.a1
pyrus_communis pycom13g29140
rosa_chinensis RchiOBHm_Chr4g0413341
rosa_multiflora Rmu_sc0007671.1_g000002 Rmu_sc0009573.1_g000009
rosa_roxburghii Rroxscaffold_5G00357260
rosa_rugosa Rorug04G0120000
rosa_samantha Rh4AG179400 Rh4BG178500 Rh4CG190000 Rh4DG174500
rosa_wichuraiana Rw4G014910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 113, 231
AcuI CTGAAG 1 cut(s) 155
AdeI CACNNNGTG 1 cut(s) 96
AfaI GTAC 1 cut(s) 185
AgsI TTSAA 1 cut(s) 205
AluBI AGCT 1 cut(s) 136
AluI AGCT 1 cut(s) 136
Alw26I GTCTC 1 cut(s) 288
Ama87I CYCGRG 2 cut(s) 70, 194
AoxI GGCC 2 cut(s) 8, 40
ApeKI GCWGC 1 cut(s) 136
AspLEI GCGC 1 cut(s) 236
AsuC2I CCSGG 2 cut(s) 195, 196
AsuHPI GGTGA 3 cut(s) 28, 254, 278
AvaI CYCGRG 2 cut(s) 70, 194
BanII GRGCYC 1 cut(s) 83
BauI CACGAG 1 cut(s) 334
BbsI GAAGAC 1 cut(s) 263
BbvI GCAGC 1 cut(s) 123
BccI CCATC 1 cut(s) 136
BcnI CCSGG 2 cut(s) 195, 196
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 1 cut(s) 237
BfoI RGCGCY 1 cut(s) 237
BisI GCNGC 2 cut(s) 137, 232
BlsI GCNGC 2 cut(s) 138, 233
Bme1390I CCNGG 2 cut(s) 195, 196
BmeT110I CYCGRG 2 cut(s) 70, 194
BmrFI CCNGG 2 cut(s) 195, 196
BpiI GAAGAC 1 cut(s) 263
BpuMI CCSGG 2 cut(s) 195, 196
BsaJI CCNNGG 3 cut(s) 69, 194, 195
Bse118I RCCGGY 1 cut(s) 38
Bse1I ACTGG 1 cut(s) 218
BseDI CCNNGG 3 cut(s) 69, 194, 195
BseNI ACTGG 1 cut(s) 218
BseRI GAGGAG 1 cut(s) 267
BseXI GCAGC 1 cut(s) 123
BsgI GTGCAG 1 cut(s) 43
BshFI GGCC 2 cut(s) 10, 42
BsiHKCI CYCGRG 2 cut(s) 70, 194
BsiSI CCGG 3 cut(s) 39, 160, 195
BsmAI GTCTC 1 cut(s) 288
BsmBI CGTCTC 1 cut(s) 288
BsnI GGCC 2 cut(s) 10, 42
BsoBI CYCGRG 2 cut(s) 70, 194
Bsp1286I GDGCHC 1 cut(s) 83
BspACI CCGC 2 cut(s) 113, 231
BspANI GGCC 2 cut(s) 10, 42
BsrFI RCCGGY 1 cut(s) 38
BsrI ACTGG 1 cut(s) 218
BssAI RCCGGY 1 cut(s) 38
BssECI CCNNGG 3 cut(s) 69, 194, 195
BssSI CACGAG 1 cut(s) 334
Bst2BI CACGAG 1 cut(s) 334
Bst4CI ACNGT 1 cut(s) 104
BstC8I GCNNGC 2 cut(s) 58, 134
BstDEI CTNAG 1 cut(s) 309
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 1 cut(s) 236
BstMAI GTCTC 1 cut(s) 288
BstMWI GCNNNNNNNGC 1 cut(s) 231
BstSCI CCNGG 2 cut(s) 193, 194
BstV1I GCAGC 1 cut(s) 123
BstV2I GAAGAC 1 cut(s) 263
BsuRI GGCC 2 cut(s) 10, 42
Cac8I GCNNGC 2 cut(s) 58, 134
CfoI GCGC 1 cut(s) 236
Cfr10I RCCGGY 1 cut(s) 38
Cfr9I CCCGGG 1 cut(s) 194
Csp6I GTAC 1 cut(s) 184
CviJI RGCY 8 cut(s) 10, 42, 81, 136, 159, 200, 208, 267
CviKI_1 RGCY 8 cut(s) 10, 42, 81, 136, 159, 200, 208, 267
CviQI GTAC 1 cut(s) 184
DdeI CTNAG 1 cut(s) 309
DraIII CACNNNGTG 1 cut(s) 96
Eco147I AGGCCT 1 cut(s) 10
Eco24I GRGCYC 1 cut(s) 83
Eco57I CTGAAG 1 cut(s) 155
Eco88I CYCGRG 2 cut(s) 70, 194
EcoT38I GRGCYC 1 cut(s) 83
Esp3I CGTCTC 1 cut(s) 288
FaiI YATR 2 cut(s) 188, 252
Fnu4HI GCNGC 2 cut(s) 137, 232
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 2 cut(s) 137, 232
FspBI CTAG 1 cut(s) 237
GlaI GCGC 1 cut(s) 235
GluI GCNGC 2 cut(s) 137, 232
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 2 cut(s) 10, 42
HapII CCGG 3 cut(s) 39, 160, 195
HhaI GCGC 1 cut(s) 236
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HinfI GANTC 2 cut(s) 146, 332
HpaII CCGG 3 cut(s) 39, 160, 195
HphI GGTGA 3 cut(s) 28, 254, 278
Hpy166II GTNNAC 1 cut(s) 90
Hpy188I TCNGA 4 cut(s) 151, 174, 297, 331
Hpy188III TCNNGA 2 cut(s) 72, 336
Hpy8I GTNNAC 1 cut(s) 90
HpyAV CCTTC 1 cut(s) 35
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4IV ACGT 1 cut(s) 86
HpyCH4V TGCA 4 cut(s) 4, 60, 99, 225
HpyF10VI GCNNNNNNNGC 1 cut(s) 231
HpyF3I CTNAG 1 cut(s) 309
HpySE526I ACGT 1 cut(s) 86
HspAI GCGC 1 cut(s) 234
LpnPI CCDG 7 cut(s) 52, 70, 173, 208, 222, 231, 246
Lsp1109I GCAGC 1 cut(s) 123
MaeI CTAG 1 cut(s) 237
MaeII ACGT 1 cut(s) 86
MboII GAAGA 3 cut(s) 161, 190, 263
MhlI GDGCHC 1 cut(s) 83
MlyI GAGTC 2 cut(s) 155, 326
MmeI TCCRAC 2 cut(s) 129, 309
MnlI CCTC 8 cut(s) 62, 79, 156, 233, 257, 285, 288, 293
MspI CCGG 3 cut(s) 39, 160, 195
MspR9I CCNGG 2 cut(s) 195, 196
MwoI GCNNNNNNNGC 1 cut(s) 231
NciI CCSGG 2 cut(s) 195, 196
PceI AGGCCT 1 cut(s) 10
PcsI WCGNNNNNNNCGW 2 cut(s) 83, 92
PkrI GCNGC 2 cut(s) 138, 233
PleI GAGTC 2 cut(s) 154, 326
PpsI GAGTC 2 cut(s) 154, 326
PsrI GAACNNNNNNTAC 2 cut(s) 67, 99
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
SatI GCNGC 2 cut(s) 137, 232
SchI GAGTC 2 cut(s) 155, 326
ScrFI CCNGG 2 cut(s) 195, 196
SduI GDGCHC 1 cut(s) 83
SetI ASST 7 cut(s) 54, 89, 138, 167, 244, 304, 329
SmaI CCCGGG 1 cut(s) 196
SseBI AGGCCT 1 cut(s) 10
SsiI CCGC 2 cut(s) 113, 231
SspMI CTAG 1 cut(s) 237
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 2 cut(s) 193, 194
TaaI ACNGT 1 cut(s) 104
TaiI ACGT 1 cut(s) 89
TaqI TCGA 1 cut(s) 107
TatI WGTACW 1 cut(s) 183
TauI GCSGC 1 cut(s) 234
TseI GCWGC 1 cut(s) 136
TspGWI ACGGA 1 cut(s) 270
TspMI CCCGGG 1 cut(s) 194
XmaI CCCGGG 1 cut(s) 194
XspI CTAG 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.