FvH4_4g13190

ralf-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015943
Physical Location & Seq
Forward (+)
16794712 .. 16795349
638 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13190.t1

Sequence Viewer

Length: 330 bp
ATGCTGTCTCTGCAATCTTGCTTCGTTCTACTACTCCTCGGCCTTTCCTATCTCGGCGCAACTACCTCATCTACATCTTCATCATCATGCAATGGTTCTATAGCAGAGTGCGAAACAGAAAGCGAGCTGCTGATGGAGTCAGAGATAAGCAGAAGGTTTCTAGAACAGAAGAAGTATATCTCACCTGGGGCTTTGAAGAAAGACCAACCAGTCTGTAACGGCGGTGCTAGAGGTGAAGCTTATAGTAAAACAGGAGGCTGTCTTCCTCCTCCGGCCAACACTTACAACAGAGGCTGCTCTAAATACTATCGGTGTAGATCCGACTCGTGA

Protein Analysis

110

Amino Acids

11.75

Weight (kDa)

8.16

Isoelectric Point (pI)

67.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 42 - 106 4.6e-25 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015943)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14010
fragaria_vesca FvH4_4g13190
malus_domestica MD04G1018100.v1.1
prunus_persica Prupe.1G167100_v2.0.a1
pyrus_communis pycom13g29140
rosa_chinensis RchiOBHm_Chr4g0413341
rosa_multiflora Rmu_sc0007671.1_g000002 Rmu_sc0009573.1_g000009
rosa_roxburghii Rroxscaffold_5G00357260
rosa_rugosa Rorug04G0120000
rosa_samantha Rh4AG179400 Rh4BG178500 Rh4CG190000 Rh4DG174500
rosa_wichuraiana Rw4G014910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 209
AciI CCGC 1 cut(s) 222
AclWI GGATC 1 cut(s) 312
AcoI YGGCCR 1 cut(s) 273
AgsI TTSAA 1 cut(s) 196
AjnI CCWGG 1 cut(s) 184
AluBI AGCT 2 cut(s) 127, 239
AluI AGCT 2 cut(s) 127, 239
Alw26I GTCTC 1 cut(s) 12
AlwI GGATC 1 cut(s) 312
AlwNI CAGNNNCTG 1 cut(s) 294
AoxI GGCC 2 cut(s) 40, 273
ApeKI GCWGC 2 cut(s) 127, 294
AspLEI GCGC 1 cut(s) 59
AsuHPI GGTGA 2 cut(s) 174, 245
BauI CACGAG 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 254
BbvI GCAGC 2 cut(s) 114, 281
BccI CCATC 1 cut(s) 127
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 2 cut(s) 161, 228
BfmI CTRYAG 1 cut(s) 99
BisI GCNGC 2 cut(s) 128, 295
BlsI GCNGC 2 cut(s) 129, 296
Bme1390I CCNGG 1 cut(s) 186
BmrFI CCNGG 1 cut(s) 186
BpiI GAAGAC 1 cut(s) 254
BsaJI CCNNGG 2 cut(s) 37, 185
Bse1I ACTGG 1 cut(s) 209
Bse3DI GCAATG 1 cut(s) 97
BseBI CCWGG 1 cut(s) 186
BseDI CCNNGG 2 cut(s) 37, 185
BseMI GCAATG 1 cut(s) 97
BseNI ACTGG 1 cut(s) 209
BseRI GAGGAG 2 cut(s) 26, 258
BseXI GCAGC 2 cut(s) 114, 281
BshFI GGCC 2 cut(s) 42, 275
BsiSI CCGG 1 cut(s) 272
BsmAI GTCTC 1 cut(s) 12
BsnI GGCC 2 cut(s) 42, 275
Bsp143I GATC 1 cut(s) 317
BspACI CCGC 1 cut(s) 222
BspANI GGCC 2 cut(s) 42, 275
BspPI GGATC 1 cut(s) 312
BsrDI GCAATG 1 cut(s) 97
BsrI ACTGG 1 cut(s) 209
BssECI CCNNGG 2 cut(s) 37, 185
BssMI GATC 1 cut(s) 317
BssSI CACGAG 1 cut(s) 325
Bst2BI CACGAG 1 cut(s) 325
Bst2UI CCWGG 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 125
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 1 cut(s) 320
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 1 cut(s) 317
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 184
BstSFI CTRYAG 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 114, 281
BstV2I GAAGAC 1 cut(s) 254
BstX2I RGATCY 1 cut(s) 317
BstYI RGATCY 1 cut(s) 317
BsuRI GGCC 2 cut(s) 42, 275
Cac8I GCNNGC 1 cut(s) 125
CaiI CAGNNNCTG 1 cut(s) 294
CfoI GCGC 1 cut(s) 59
CviAII CATG 1 cut(s) 87
CviJI RGCY 7 cut(s) 42, 127, 191, 239, 258, 275, 294
CviKI_1 RGCY 7 cut(s) 42, 127, 191, 239, 258, 275, 294
DpnI GATC 1 cut(s) 319
DpnII GATC 1 cut(s) 317
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 184
FaeI CATG 1 cut(s) 90
FaiI YATR 4 cut(s) 88, 101, 177, 243
FatI CATG 1 cut(s) 86
Fnu4HI GCNGC 2 cut(s) 128, 295
Fsp4HI GCNGC 2 cut(s) 128, 295
FspBI CTAG 2 cut(s) 161, 228
GlaI GCGC 1 cut(s) 58
GluI GCNGC 2 cut(s) 128, 295
HaeIII GGCC 2 cut(s) 42, 275
HapII CCGG 1 cut(s) 272
HhaI GCGC 1 cut(s) 59
Hin1II CATG 1 cut(s) 90
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HindIII AAGCTT 1 cut(s) 237
HinfI GANTC 2 cut(s) 137, 323
HpaII CCGG 1 cut(s) 272
HphI GGTGA 2 cut(s) 174, 245
Hpy188I TCNGA 2 cut(s) 142, 322
Hpy188III TCNNGA 2 cut(s) 161, 327
HpyAV CCTTC 1 cut(s) 147
HpyCH4V TGCA 2 cut(s) 13, 90
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
Hsp92II CATG 1 cut(s) 90
HspAI GCGC 1 cut(s) 57
Kzo9I GATC 1 cut(s) 317
LpnPI CCDG 5 cut(s) 171, 198, 222, 237, 285
Lsp1109I GCAGC 2 cut(s) 114, 281
MaeI CTAG 2 cut(s) 161, 228
MaeIII GTNAC 1 cut(s) 215
MalI GATC 1 cut(s) 319
MboI GATC 1 cut(s) 317
MboII GAAGA 4 cut(s) 69, 181, 208, 254
MflI RGATCY 1 cut(s) 317
MlyI GAGTC 2 cut(s) 146, 317
MnlI CCTC 7 cut(s) 47, 76, 224, 248, 276, 279, 284
MslI CAYNNNNRTG 1 cut(s) 85
MspI CCGG 1 cut(s) 272
MspR9I CCNGG 1 cut(s) 186
MvaI CCWGG 1 cut(s) 186
MwoI GCNNNNNNNGC 1 cut(s) 10
NdeII GATC 1 cut(s) 317
NlaIII CATG 1 cut(s) 90
NmeAIII GCCGAG 2 cut(s) 18, 33
PkrI GCNGC 2 cut(s) 129, 296
PleI GAGTC 2 cut(s) 145, 317
PpsI GAGTC 2 cut(s) 145, 317
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PstNI CAGNNNCTG 1 cut(s) 294
PsuI RGATCY 1 cut(s) 317
RseI CAYNNNNRTG 1 cut(s) 85
SatI GCNGC 2 cut(s) 128, 295
Sau3AI GATC 1 cut(s) 317
SchI GAGTC 2 cut(s) 146, 317
ScrFI CCNGG 1 cut(s) 186
SetI ASST 6 cut(s) 68, 129, 158, 187, 235, 241
SfcI CTRYAG 1 cut(s) 99
SmiMI CAYNNNNRTG 1 cut(s) 85
SsiI CCGC 1 cut(s) 222
SspMI CTAG 2 cut(s) 161, 228
StyD4I CCNGG 1 cut(s) 184
TseI GCWGC 2 cut(s) 127, 294
TspDTI ATGAA 1 cut(s) 69
XbaI TCTAGA 1 cut(s) 160
XspI CTAG 2 cut(s) 161, 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.