Rh4BG178500

ralf-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0015943
Physical Location & Seq
Forward (+)
31649695 .. 31650993
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG178500.1

Sequence Viewer

Length: 327 bp
ATGGTGTCTCTGCAATCTTGCTTCGTTCTACTACTTCTTTGCCTTTCCTATCTCAGCGCAACTGCCTCACCACCATCATCATCAACATGCAATGGTTCCATAGCAGAGTGTGAAACAGAAAGCGAGCTGCTGATGGACTCCGAAATAAGCAGAAGGTTTCTCGCACAGAAGTACATCTCACCTGGAGCTTTGAGGAAAGACCAACCAGTCTGCAACGGCGGTGCTAGTGGCGAAGCTTACAGTAAAACAGGAGGCTGTCTTCCTCCTCCGGCTAACCCTTATAACAGAGGTTGCTCTAAATACTATCGGTGTAGATCCGACTCGTGA

Protein Analysis

108

Amino Acids

11.56

Weight (kDa)

7.47

Isoelectric Point (pI)

74.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 42 - 105 5.6e-25 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015943)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14010
fragaria_vesca FvH4_4g13190
malus_domestica MD04G1018100.v1.1
prunus_persica Prupe.1G167100_v2.0.a1
pyrus_communis pycom13g29140
rosa_chinensis RchiOBHm_Chr4g0413341
rosa_multiflora Rmu_sc0007671.1_g000002 Rmu_sc0009573.1_g000009
rosa_roxburghii Rroxscaffold_5G00357260
rosa_rugosa Rorug04G0120000
rosa_samantha Rh4AG179400 Rh4BG178500 Rh4CG190000 Rh4DG174500
rosa_wichuraiana Rw4G014910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 282
AasI GACNNNNNNGTC 1 cut(s) 206
AciI CCGC 1 cut(s) 219
AclWI GGATC 1 cut(s) 309
AfaI GTAC 1 cut(s) 173
AjnI CCWGG 1 cut(s) 181
AluBI AGCT 3 cut(s) 127, 188, 236
AluI AGCT 3 cut(s) 127, 188, 236
Alw26I GTCTC 1 cut(s) 12
AlwI GGATC 1 cut(s) 309
ApeKI GCWGC 1 cut(s) 127
AspLEI GCGC 1 cut(s) 59
AsuHPI GGTGA 2 cut(s) 60, 171
BauI CACGAG 1 cut(s) 322
BbsI GAAGAC 1 cut(s) 251
BbvI GCAGC 1 cut(s) 114
BccI CCATC 2 cut(s) 82, 127
BceAI ACGGC 1 cut(s) 232
BciT130I CCWGG 1 cut(s) 183
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 1 cut(s) 225
BisI GCNGC 1 cut(s) 128
BlsI GCNGC 1 cut(s) 129
Bme1390I CCNGG 1 cut(s) 183
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 1 cut(s) 183
BpiI GAAGAC 1 cut(s) 251
BpmI CTGGAG 1 cut(s) 204
Bse1I ACTGG 1 cut(s) 206
Bse3DI GCAATG 1 cut(s) 97
BseBI CCWGG 1 cut(s) 183
BseMI GCAATG 1 cut(s) 97
BseMII CTCAG 1 cut(s) 67
BseNI ACTGG 1 cut(s) 206
BseRI GAGGAG 1 cut(s) 255
BseXI GCAGC 1 cut(s) 114
BsiSI CCGG 1 cut(s) 269
BsmAI GTCTC 1 cut(s) 12
Bsp143I GATC 1 cut(s) 314
BspACI CCGC 1 cut(s) 219
BspCNI CTCAG 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 97
BspPI GGATC 1 cut(s) 309
BsrDI GCAATG 1 cut(s) 97
BsrI ACTGG 1 cut(s) 206
BssMI GATC 1 cut(s) 314
BssSI CACGAG 1 cut(s) 322
Bst2BI CACGAG 1 cut(s) 322
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 1 cut(s) 242
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 1 cut(s) 53
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 1 cut(s) 317
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 1 cut(s) 314
BstNI CCWGG 1 cut(s) 183
BstNSI RCATGY 1 cut(s) 90
BstSCI CCNGG 1 cut(s) 181
BstV1I GCAGC 1 cut(s) 114
BstV2I GAAGAC 1 cut(s) 251
BstX2I RGATCY 1 cut(s) 314
BstYI RGATCY 1 cut(s) 314
Cac8I GCNNGC 1 cut(s) 125
CfoI GCGC 1 cut(s) 59
Csp6I GTAC 1 cut(s) 172
CviAII CATG 1 cut(s) 87
CviJI RGCY 5 cut(s) 127, 188, 236, 255, 272
CviKI_1 RGCY 5 cut(s) 127, 188, 236, 255, 272
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 1 cut(s) 53
DpnI GATC 1 cut(s) 316
DpnII GATC 1 cut(s) 314
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 181
FaeI CATG 1 cut(s) 90
FaiI YATR 3 cut(s) 88, 101, 282
FatI CATG 1 cut(s) 86
Fnu4HI GCNGC 1 cut(s) 128
Fsp4HI GCNGC 1 cut(s) 128
FspBI CTAG 1 cut(s) 225
GlaI GCGC 1 cut(s) 58
GluI GCNGC 1 cut(s) 128
GsuI CTGGAG 1 cut(s) 204
HapII CCGG 1 cut(s) 269
HhaI GCGC 1 cut(s) 59
Hin1II CATG 1 cut(s) 90
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HindIII AAGCTT 1 cut(s) 234
HinfI GANTC 2 cut(s) 137, 320
HpaII CCGG 1 cut(s) 269
HphI GGTGA 2 cut(s) 60, 171
Hpy188I TCNGA 2 cut(s) 142, 319
Hpy188III TCNNGA 1 cut(s) 324
HpyAV CCTTC 1 cut(s) 147
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4V TGCA 3 cut(s) 13, 90, 213
HpyF3I CTNAG 1 cut(s) 53
Hsp92II CATG 1 cut(s) 90
HspAI GCGC 1 cut(s) 57
Kzo9I GATC 1 cut(s) 314
LmnI GCTCC 1 cut(s) 185
LpnPI CCDG 5 cut(s) 168, 195, 219, 234, 282
Lsp1109I GCAGC 1 cut(s) 114
MaeI CTAG 1 cut(s) 225
MalI GATC 1 cut(s) 316
MboI GATC 1 cut(s) 314
MboII GAAGA 1 cut(s) 251
MflI RGATCY 1 cut(s) 314
MlyI GAGTC 2 cut(s) 131, 314
MnlI CCTC 6 cut(s) 76, 186, 245, 273, 276, 281
MslI CAYNNNNRTG 1 cut(s) 85
MspI CCGG 1 cut(s) 269
MspR9I CCNGG 1 cut(s) 183
MvaI CCWGG 1 cut(s) 183
NdeII GATC 1 cut(s) 314
NlaIII CATG 1 cut(s) 90
NlaIV GGNNCC 1 cut(s) 97
NspI RCATGY 1 cut(s) 90
PkrI GCNGC 1 cut(s) 129
PleI GAGTC 2 cut(s) 131, 314
PpsI GAGTC 2 cut(s) 131, 314
PsiI TTATAA 1 cut(s) 282
Psp6I CCWGG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 181
PspN4I GGNNCC 1 cut(s) 97
PsuI RGATCY 1 cut(s) 314
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
RseI CAYNNNNRTG 1 cut(s) 85
SatI GCNGC 1 cut(s) 128
Sau3AI GATC 1 cut(s) 314
SchI GAGTC 2 cut(s) 131, 314
ScrFI CCNGG 1 cut(s) 183
SetI ASST 6 cut(s) 129, 158, 184, 190, 238, 292
SmiMI CAYNNNNRTG 1 cut(s) 85
SsiI CCGC 1 cut(s) 219
SspMI CTAG 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 181
TaaI ACNGT 1 cut(s) 242
TatI WGTACW 1 cut(s) 171
TseI GCWGC 1 cut(s) 127
XceI RCATGY 1 cut(s) 90
XspI CTAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.