AT4G14750

IQ-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010897
Physical Location & Seq
Forward (+)
8470179 .. 8472217
2039 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G14750.1

Sequence Viewer

Length: 1164 bp
ATGGGGAAAACTAGCAAATGGTTTCGAAGTCTCTTAACCGGAAAGAAAGAGAGAACAAAGGAACATATCATTCAAAGCGAGTGTGTATTCACTTCTTCCATTCCAGGCACTCCTAAGGAGAAACGTCGATGGAGTTTTCGACGTTCTTCAGCAACTGGACCACCGCCACCAGCTTGTGCTATTACACTGAAAGATTCACCACCACCGCCGCCACCACCACCTCCACCTCCACCGCTACAACAACCATTTGTGGTCGAAATCGTGGACAATGAAGATGAACAAATCAAGAACGTTTCAGCTGAAGAGATTGAAGAATTTGCAGCAATAAAGATTCAAGCGTGCTATCGTTCTCATTTGGCGAGGAAAGCATTAAGAGCATTAAAAGGGTTAGTGAAATTACAAGCACTAGTGAGAGGTCATTTAGTAAGGAAACAAGCAACAGCTACACTTAGATGTATGCAAGCATTAATAACACTTCAAGCCAAAGCTCGAGAACAGAGGATTCGTATGATCGGAGGAGATTCAACAAATCCAAGAACATCGATTCACAAAACCAGGATCAATAACTTTTACCACGAGAATGAAGAAAACATCAAGATTGTGGAAATGGACATTCAATCGAAAATGTACTCTCCGGCTCCTTCGGCTTTAACCGAGATGAGTCCTCGCGCGTATAGTAGCCATTTCGAGGATTGTAACTCATTCAACACAGCTCAAAGTAGTCCACAATGTTTCAGCAGATTCAAAGAGTATTACAATGGAGATACTTTGTCTTCTTATGATTACCCTCTCTTCCCTAATTACATGGCTAATACTCAGTCTTCTAAAGCCAAGGCTCGGTCTCAAAGTGCGCCGAAGCAGAGACCTCCTGAGATCTATGAGAAGCAGATGAGTGGGAGGAGAAGATCTTCGATGGAAGCACCGAGGAATAACGGTGTCCCGAGAGCTGTAAGGATGCAGAGATCTTCATCTCAACTAGGGTCAAACACAGCCAAGGAAAGTCAACAACATCATCATCATCAGTACTATCCGTGGATGGCGATAAAGCTCGATAGATCTAATATTTCGCTTATGGAGAGCGAATGCGGATCTACAAGTACCGTTATGACTAATACCAACTACGGTAGACATGTTGATGTTCAGGGAAACAACAACATGTACTGA

Protein Analysis

387

Amino Acids

43.91

Weight (kDa)

9.7

Isoelectric Point (pI)

81.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 238 - 318 9.4e-13 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010897)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14750 AT4G14750 AT4G14750
fragaria_vesca FvH4_4g01000 FvH4_4g01000
malus_domestica MD13G1198300.v1.1 MD16G1198400.v1.1
prunus_persica Prupe.1G020000_v2.0.a1
pyrus_communis pycom13g17210 pycom16g16730
rosa_chinensis RchiOBHm_Chr4g0387551
rosa_laevigata RLG00000010112
rosa_multiflora Rmu_sc0007248.1_g000016
rosa_roxburghii Rroxscaffold_5G00334230
rosa_rugosa Rorug03G0310600
rosa_samantha Rh4AG016600 Rh4BG011500 Rh4CG017800 Rh4DG012400
rosa_wichuraiana Rw4G001060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1126
AccII CGCG 2 cut(s) 669, 671
AciI CCGC 5 cut(s) 164, 206, 209, 233, 1086
AclI AACGTT 1 cut(s) 291
AclWI GGATC 2 cut(s) 566, 1096
AcsI RAATTY 1 cut(s) 314
AcuI CTGAAG 2 cut(s) 132, 321
AfaI GTAC 4 cut(s) 629, 1025, 1099, 1160
AfiI CCNNNNNNNGG 2 cut(s) 688, 837
AflIII ACRYGT 2 cut(s) 1129, 1155
AgsI TTSAA 8 cut(s) 74, 311, 335, 479, 525, 617, 706, 745
AhlI ACTAGT 1 cut(s) 406
AjnI CCWGG 2 cut(s) 103, 554
AluBI AGCT 7 cut(s) 173, 299, 443, 488, 713, 947, 1048
AluI AGCT 7 cut(s) 173, 299, 443, 488, 713, 947, 1048
Alw26I GTCTC 3 cut(s) 35, 846, 856
AlwI GGATC 2 cut(s) 566, 1096
AlwNI CAGNNNCTG 1 cut(s) 155
Ama87I CYCGRG 2 cut(s) 489, 940
ApeKI GCWGC 1 cut(s) 320
ApoI RAATTY 1 cut(s) 314
AseI ATTAAT 1 cut(s) 467
Asp700I GAANNNNTTC 1 cut(s) 907
AspLEI GCGC 2 cut(s) 671, 853
AspS9I GGNCC 1 cut(s) 158
AsuHPI GGTGA 1 cut(s) 189
AsuII TTCGAA 1 cut(s) 25
AvaI CYCGRG 2 cut(s) 489, 940
AvaII GGWCC 1 cut(s) 158
AxyI CCTNAGG 1 cut(s) 114
BauI CACGAG 1 cut(s) 575
BbsI GAAGAC 2 cut(s) 765, 813
BbvI GCAGC 1 cut(s) 332
BccI CCATC 3 cut(s) 123, 907, 1030
BciT130I CCWGG 2 cut(s) 105, 556
BcoDI GTCTC 3 cut(s) 35, 846, 856
BcuI ACTAGT 1 cut(s) 406
BfaI CTAG 3 cut(s) 12, 407, 977
BglII AGATCT 4 cut(s) 873, 905, 962, 1055
BisI GCNGC 2 cut(s) 209, 321
BlsI GCNGC 2 cut(s) 210, 322
BmcAI AGTACT 1 cut(s) 1025
Bme1390I CCNGG 2 cut(s) 105, 556
Bme18I GGWCC 1 cut(s) 158
BmeT110I CYCGRG 2 cut(s) 489, 940
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 1 cut(s) 639
BmrFI CCNGG 2 cut(s) 105, 556
BmsI GCATC 1 cut(s) 945
BpiI GAAGAC 2 cut(s) 765, 813
Bpu14I TTCGAA 1 cut(s) 25
Bsa29I ATCGAT 1 cut(s) 542
BsaBI GATNNNNATC 1 cut(s) 967
BsaI GGTCTC 2 cut(s) 846, 856
BsaJI CCNNGG 4 cut(s) 831, 923, 993, 1031
BsaWI WCCGGW 1 cut(s) 38
Bsc4I CCNNNNNNNGG 2 cut(s) 688, 837
Bse1I ACTGG 1 cut(s) 160
Bse21I CCTNAGG 1 cut(s) 114
Bse8I GATNNNNATC 1 cut(s) 967
BseBI CCWGG 2 cut(s) 105, 556
BseCI ATCGAT 1 cut(s) 542
BseDI CCNNGG 4 cut(s) 831, 923, 993, 1031
BseGI GGATG 2 cut(s) 960, 1041
BseJI GATNNNNATC 1 cut(s) 967
BseLI CCNNNNNNNGG 2 cut(s) 688, 837
BseMII CTCAG 2 cut(s) 830, 861
BseNI ACTGG 1 cut(s) 160
BseRI GAGGAG 2 cut(s) 531, 913
BseXI GCAGC 1 cut(s) 332
Bsh1236I CGCG 2 cut(s) 669, 671
BshVI ATCGAT 1 cut(s) 542
BsiHKCI CYCGRG 2 cut(s) 489, 940
BsiSI CCGG 2 cut(s) 39, 635
BslFI GGGAC 1 cut(s) 923
BslI CCNNNNNNNGG 2 cut(s) 688, 837
BsmAI GTCTC 3 cut(s) 35, 846, 856
BsmFI GGGAC 1 cut(s) 923
BsmI GAATGC 1 cut(s) 1088
Bso31I GGTCTC 2 cut(s) 846, 856
BsoBI CYCGRG 2 cut(s) 489, 940
Bsp119I TTCGAA 1 cut(s) 25
Bsp143I GATC 7 cut(s) 510, 558, 873, 905, 962, 1055, 1088
BspACI CCGC 5 cut(s) 164, 206, 209, 233, 1086
BspCNI CTCAG 2 cut(s) 829, 862
BspDI ATCGAT 1 cut(s) 542
BspFNI CGCG 2 cut(s) 669, 671
BspLI GGNNCC 1 cut(s) 639
BspPI GGATC 2 cut(s) 566, 1096
BspT104I TTCGAA 1 cut(s) 25
BspTNI GGTCTC 2 cut(s) 846, 856
BsrI ACTGG 1 cut(s) 160
BssECI CCNNGG 4 cut(s) 831, 923, 993, 1031
BssMI GATC 7 cut(s) 510, 558, 873, 905, 962, 1055, 1088
BssSI CACGAG 1 cut(s) 575
BssT1I CCWWGG 2 cut(s) 831, 993
Bst2BI CACGAG 1 cut(s) 575
Bst2UI CCWGG 2 cut(s) 105, 556
Bst4CI ACNGT 3 cut(s) 935, 1102, 1124
Bst6I CTCTTC 2 cut(s) 297, 797
BstBI TTCGAA 1 cut(s) 25
BstC8I GCNNGC 2 cut(s) 340, 462
BstDEI CTNAG 4 cut(s) 114, 449, 816, 870
BstDSI CCRYGG 1 cut(s) 1031
BstF5I GGATG 2 cut(s) 960, 1041
BstFNI CGCG 2 cut(s) 669, 671
BstHHI GCGC 2 cut(s) 671, 853
BstKTI GATC 7 cut(s) 513, 561, 876, 908, 965, 1058, 1091
BstMAI GTCTC 3 cut(s) 35, 846, 856
BstMBI GATC 7 cut(s) 510, 558, 873, 905, 962, 1055, 1088
BstMWI GCNNNNNNNGC 3 cut(s) 365, 374, 644
BstNI CCWGG 2 cut(s) 105, 556
BstNSI RCATGY 2 cut(s) 1133, 1159
BstSCI CCNGG 2 cut(s) 103, 554
BstUI CGCG 2 cut(s) 669, 671
BstV1I GCAGC 1 cut(s) 332
BstV2I GAAGAC 2 cut(s) 765, 813
BstX2I RGATCY 5 cut(s) 873, 905, 962, 1055, 1088
BstYI RGATCY 5 cut(s) 873, 905, 962, 1055, 1088
Bsu15I ATCGAT 1 cut(s) 542
Bsu36I CCTNAGG 1 cut(s) 114
BsuTUI ATCGAT 1 cut(s) 542
BtgI CCRYGG 1 cut(s) 1031
BtsCI GGATG 2 cut(s) 960, 1041
BtsIMutI CAGTG 1 cut(s) 185
Cac8I GCNNGC 2 cut(s) 340, 462
CaiI CAGNNNCTG 1 cut(s) 155
CfoI GCGC 2 cut(s) 671, 853
Cfr13I GGNCC 1 cut(s) 158
ClaI ATCGAT 1 cut(s) 542
Csp6I GTAC 4 cut(s) 628, 1024, 1098, 1159
CviAII CATG 3 cut(s) 805, 1130, 1156
CviQI GTAC 4 cut(s) 628, 1024, 1098, 1159
DdeI CTNAG 4 cut(s) 114, 449, 816, 870
DpnI GATC 7 cut(s) 512, 560, 875, 907, 964, 1057, 1090
DpnII GATC 7 cut(s) 510, 558, 873, 905, 962, 1055, 1088
Eam1104I CTCTTC 2 cut(s) 297, 797
EarI CTCTTC 2 cut(s) 297, 797
Eco130I CCWWGG 2 cut(s) 831, 993
Eco31I GGTCTC 2 cut(s) 846, 856
Eco47I GGWCC 1 cut(s) 158
Eco57I CTGAAG 2 cut(s) 132, 321
Eco81I CCTNAGG 1 cut(s) 114
Eco88I CYCGRG 2 cut(s) 489, 940
EcoRII CCWGG 2 cut(s) 103, 554
EcoT14I CCWWGG 2 cut(s) 831, 993
ErhI CCWWGG 2 cut(s) 831, 993
FaeI CATG 3 cut(s) 808, 1133, 1159
FaqI GGGAC 1 cut(s) 923
FatI CATG 3 cut(s) 804, 1129, 1155
FblI GTMKAC 1 cut(s) 1126
Fnu4HI GCNGC 2 cut(s) 209, 321
FokI GGATG 2 cut(s) 967, 1048
Fsp4HI GCNGC 2 cut(s) 209, 321
FspBI CTAG 3 cut(s) 12, 407, 977
GlaI GCGC 2 cut(s) 670, 852
GluI GCNGC 2 cut(s) 209, 321
HapII CCGG 2 cut(s) 39, 635
HhaI GCGC 2 cut(s) 671, 853
Hin1II CATG 3 cut(s) 808, 1133, 1159
Hin6I GCGC 2 cut(s) 669, 851
HinP1I GCGC 2 cut(s) 669, 851
HincII GTYRAC 1 cut(s) 1004
HindII GTYRAC 1 cut(s) 1004
HinfI GANTC 7 cut(s) 194, 331, 502, 521, 544, 661, 741
HpaII CCGG 2 cut(s) 39, 635
HphI GGTGA 1 cut(s) 189
Hpy166II GTNNAC 4 cut(s) 265, 725, 1004, 1127
Hpy188I TCNGA 1 cut(s) 515
Hpy188III TCNNGA 5 cut(s) 286, 491, 595, 869, 940
Hpy8I GTNNAC 4 cut(s) 265, 725, 1004, 1127
Hpy99I CGWCG 2 cut(s) 129, 144
HpyAV CCTTC 1 cut(s) 651
HpyCH4III ACNGT 3 cut(s) 935, 1102, 1124
HpyCH4IV ACGT 3 cut(s) 124, 142, 291
HpyCH4V TGCA 3 cut(s) 320, 460, 958
HpyF10VI GCNNNNNNNGC 3 cut(s) 365, 374, 644
HpyF3I CTNAG 4 cut(s) 114, 449, 816, 870
HpySE526I ACGT 3 cut(s) 124, 142, 291
Hsp92II CATG 3 cut(s) 808, 1133, 1159
HspAI GCGC 2 cut(s) 669, 851
Kzo9I GATC 7 cut(s) 510, 558, 873, 905, 962, 1055, 1088
LmnI GCTCC 1 cut(s) 643
Lsp1109I GCAGC 1 cut(s) 332
LweI GCATC 1 cut(s) 945
MaeI CTAG 3 cut(s) 12, 407, 977
MaeII ACGT 3 cut(s) 124, 142, 291
MaeIII GTNAC 1 cut(s) 695
MalI GATC 7 cut(s) 512, 560, 875, 907, 964, 1057, 1090
MboI GATC 7 cut(s) 510, 558, 873, 905, 962, 1055, 1088
MflI RGATCY 5 cut(s) 873, 905, 962, 1055, 1088
MluCI AATT 3 cut(s) 314, 395, 799
MlyI GAGTC 1 cut(s) 670
MroXI GAANNNNTTC 1 cut(s) 907
MseI TTAA 5 cut(s) 35, 371, 380, 467, 650
MslI CAYNNNNRTG 3 cut(s) 451, 579, 1134
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 2 cut(s) 39, 635
MspR9I CCNGG 2 cut(s) 105, 556
Mva1269I GAATGC 1 cut(s) 1088
MvaI CCWGG 2 cut(s) 105, 556
MvnI CGCG 2 cut(s) 669, 671
MwoI GCNNNNNNNGC 3 cut(s) 365, 374, 644
NdeII GATC 7 cut(s) 510, 558, 873, 905, 962, 1055, 1088
NlaIII CATG 3 cut(s) 808, 1133, 1159
NlaIV GGNNCC 1 cut(s) 639
NspI RCATGY 2 cut(s) 1133, 1159
NspV TTCGAA 1 cut(s) 25
PaeR7I CTCGAG 1 cut(s) 489
PciI ACATGT 2 cut(s) 1129, 1155
PctI GAATGC 1 cut(s) 1088
PdmI GAANNNNTTC 1 cut(s) 907
PfeI GAWTC 6 cut(s) 194, 331, 502, 521, 544, 741
PkrI GCNGC 2 cut(s) 210, 322
PleI GAGTC 1 cut(s) 669
PpsI GAGTC 1 cut(s) 669
PscI ACATGT 2 cut(s) 1129, 1155
PshBI ATTAAT 1 cut(s) 467
Psp1406I AACGTT 1 cut(s) 291
Psp6I CCWGG 2 cut(s) 103, 554
PspGI CCWGG 2 cut(s) 103, 554
PspN4I GGNNCC 1 cut(s) 639
PspPI GGNCC 1 cut(s) 158
PstNI CAGNNNCTG 1 cut(s) 155
PsuI RGATCY 5 cut(s) 873, 905, 962, 1055, 1088
PvuII CAGCTG 1 cut(s) 299
RsaI GTAC 4 cut(s) 629, 1025, 1099, 1160
RsaNI GTAC 4 cut(s) 628, 1024, 1098, 1159
RseI CAYNNNNRTG 3 cut(s) 451, 579, 1134
SaqAI TTAA 5 cut(s) 35, 371, 380, 467, 650
SatI GCNGC 2 cut(s) 209, 321
Sau3AI GATC 7 cut(s) 510, 558, 873, 905, 962, 1055, 1088
Sau96I GGNCC 1 cut(s) 158
ScaI AGTACT 1 cut(s) 1025
SchI GAGTC 1 cut(s) 670
ScrFI CCNGG 2 cut(s) 105, 556
SfaNI GCATC 1 cut(s) 945
Sfr274I CTCGAG 1 cut(s) 489
SfuI TTCGAA 1 cut(s) 25
SinI GGWCC 1 cut(s) 158
SlaI CTCGAG 1 cut(s) 489
SmiMI CAYNNNNRTG 3 cut(s) 451, 579, 1134
SmlI CTYRAG 1 cut(s) 489
SmoI CTYRAG 1 cut(s) 489
SpeI ACTAGT 1 cut(s) 406
Sse9I AATT 3 cut(s) 314, 395, 799
SsiI CCGC 5 cut(s) 164, 206, 209, 233, 1086
SspI AATATT 1 cut(s) 1063
SspMI CTAG 3 cut(s) 12, 407, 977
StyD4I CCNGG 2 cut(s) 103, 554
StyI CCWWGG 2 cut(s) 831, 993
TaaI ACNGT 3 cut(s) 935, 1102, 1124
TaiI ACGT 3 cut(s) 127, 145, 294
TaqII GACCGA 1 cut(s) 828
TasI AATT 3 cut(s) 314, 395, 799
TatI WGTACW 3 cut(s) 627, 1023, 1158
TauI GCSGC 1 cut(s) 211
TfiI GAWTC 6 cut(s) 194, 331, 502, 521, 544, 741
Tru1I TTAA 5 cut(s) 35, 371, 380, 467, 650
Tru9I TTAA 5 cut(s) 35, 371, 380, 467, 650
TscAI CASTG 1 cut(s) 192
TseI GCWGC 1 cut(s) 320
TspDTI ATGAA 4 cut(s) 285, 291, 597, 957
TspGWI ACGGA 1 cut(s) 1020
TspRI CASTG 1 cut(s) 192
VpaK11BI GGWCC 1 cut(s) 158
VspI ATTAAT 1 cut(s) 467
XapI RAATTY 1 cut(s) 314
XceI RCATGY 2 cut(s) 1133, 1159
XhoI CTCGAG 1 cut(s) 489
XmiI GTMKAC 1 cut(s) 1126
XmnI GAANNNNTTC 1 cut(s) 907
XspI CTAG 3 cut(s) 12, 407, 977
ZrmI AGTACT 1 cut(s) 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.