Rh4BG011500

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0010897
Physical Location & Seq
Reverse (-)
1608358 .. 1610531
2174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG011500.1

Sequence Viewer

Length: 1452 bp
ATGGGAAAGACAGGCAAATGGCTTAGAAGCTTCTTGACAGGAAAGAAAGACAGAGATCACAAGGACAAGGGAAAATGTCCAACTACTACTACTACCACCACCACCACTAATCAGAATTGCTCACCTGCCACTACTCACGATCAGAATCCCACCACTCCAAATTCAACACCAAAGGAGAAGAGGAGGTGGAGTTTCAGAAGGTCATCTGCCACATCCCCAGCTTCAAAGGACTCAAATGCTGTAGAAGCACCAACCACACCACCACCTGTGCACTCAACATTGGATTTCTCTGATAATTATGAGCAGAAAAAACATGCCTTGGCAGTGGCAGCAGCCACGGCTGCTGTTGCTGATGCAACCGCAGCTGCTGCACAAGCTGCAGCTGCTGTCATGCGGCTGACAGCAGCTGCCAATGAGAAAACTGCCGCCATTGAAGAGGCGGCCGCCATCAAAATTCAATCCGTATTTCGTTCTTATCTGGCAAGAAAAGCATTGTGTGCATTGAAAGGACTAGTAAAGTTGCAGGCATTGGTAAGGGGTCACCTAGTGAGAAAACAGGCCAAAGCCACGCTGCGGTGCATGCAGGCATTGGTGACAGCACAGGCTCGAGCTCGAGCTCAGAGGATTCGGATGGTTGAAGATGGAAATACTGTTAATCCTAGGCCATCCACCGGCAGAAGGTCTTTACAGGACAATCGGCTCAGGCATTCTTATAATGAATTTGATAGAGGCATGGAAGAGAACATTAAGATTGTGGAGATGGATCTTGGGGAATCAAAAGGAAATGTGAAGGGCAGAAACAGCTACTCGAATCACACACAAGCCGATCGACCAGACTCTAGATTTTCGACTCAGTCTGCACCAAATCATCAACCATACATAAAACCAGACAACTGCAGCAATGTCTCACCAGCTCCATCAGCTCTGACTCAGGACATGATGAGTCCAGGAGCCTGCAGTGGCCATTTCGAGGACTTTTCCTTCGGAACAGCACAAAGCAGCCCCCAGTGCTACTCTGCAATTTCCAAACCGGATCCATCAAGAGTTCCTTTCGCTTTTCCGAGGCCAGATTATGCAGAACAATTGTCCTATGACTACCCATTGTACCCAAATTACATGGCCAATACACAATCTTCAAAGGCCAAAGCCAGATCACAGAGTGCACCAAAGCAAAGACCTCCAGATGCATTTGAGAGACAACCAAGCCGACCGCGAAGGACATCAATGGAAGGAAGGAATCATATCCCAAGGTCTGTGAAGATGCAGAGGTCATCTTCACATGTAGGTTCCACCGCTCAAAATTACCAGTACCCTTGGTATAATATCAAGCTGGATAGATCCTCAGTTTCACTAATGGAAAGTGAGTGTGGCTCCAGCAGTACTGTGCTCACCACAAATACCAACTACTGCAAATCTCTTGTTGCATTTAATGGACATGGAAGTAGGTACTAA

Protein Analysis

483

Amino Acids

53.12

Weight (kDa)

9.84

Isoelectric Point (pI)

57.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IQ PF00612 147 - 165 6.3e-06 IQ calmodulin-binding motif
DUF4005 PF13178 353 - 422 2.8e-09 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010897)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14750 AT4G14750 AT4G14750
fragaria_vesca FvH4_4g01000 FvH4_4g01000
malus_domestica MD13G1198300.v1.1 MD16G1198400.v1.1
prunus_persica Prupe.1G020000_v2.0.a1
pyrus_communis pycom13g17210 pycom16g16730
rosa_chinensis RchiOBHm_Chr4g0387551
rosa_laevigata RLG00000010112
rosa_multiflora Rmu_sc0007248.1_g000016
rosa_roxburghii Rroxscaffold_5G00334230
rosa_rugosa Rorug03G0310600
rosa_samantha Rh4AG016600 Rh4BG011500 Rh4CG017800 Rh4DG012400
rosa_wichuraiana Rw4G001060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
AarI CACCTGC 1 cut(s) 133
Acc36I ACCTGC 1 cut(s) 133
AccBSI CCGCTC 1 cut(s) 1295
AccII CGCG 1 cut(s) 1213
AciI CCGC 8 cut(s) 360, 394, 426, 440, 444, 574, 1211, 1293
AclWI GGATC 4 cut(s) 771, 1028, 1041, 1332
AcoI YGGCCR 3 cut(s) 441, 961, 1119
AcsI RAATTY 3 cut(s) 160, 453, 719
AdeI CACNNNGTG 2 cut(s) 547, 1160
AfaI GTAC 4 cut(s) 1106, 1310, 1381, 1448
AfiI CCNNNNNNNGG 4 cut(s) 573, 671, 678, 970
AflIII ACRYGT 1 cut(s) 1279
AgsI TTSAA 7 cut(s) 165, 225, 434, 458, 505, 638, 1137
AhlI ACTAGT 1 cut(s) 511
AjnI CCWGG 1 cut(s) 946
Alw21I GWGCWC 5 cut(s) 273, 613, 619, 1165, 1389
Alw26I GTCTC 2 cut(s) 910, 1189
Alw44I GTGCAC 2 cut(s) 269, 1161
AlwI GGATC 4 cut(s) 771, 1028, 1041, 1332
AlwNI CAGNNNCTG 3 cut(s) 368, 386, 407
Ama87I CYCGRG 2 cut(s) 606, 612
AoxI GGCC 7 cut(s) 441, 558, 662, 961, 1064, 1119, 1140
ApaLI GTGCAC 2 cut(s) 269, 1161
ApoI RAATTY 3 cut(s) 160, 453, 719
ArsI GACNNNNNNTTYG 2 cut(s) 965, 997
AspA2I CCTAGG 1 cut(s) 659
AsuHPI GGTGA 5 cut(s) 114, 533, 604, 900, 1381
AvaI CYCGRG 2 cut(s) 606, 612
AvrII CCTAGG 1 cut(s) 659
BaeGI GKGCMC 2 cut(s) 273, 1165
BalI TGGCCA 2 cut(s) 963, 1121
BamHI GGATCC 1 cut(s) 1033
BanII GRGCYC 2 cut(s) 613, 619
Bbv12I GWGCWC 5 cut(s) 273, 613, 619, 1165, 1389
BccI CCATC 7 cut(s) 455, 625, 635, 673, 754, 925, 1045
BceAI ACGGC 1 cut(s) 354
BciT130I CCWGG 1 cut(s) 948
BcoDI GTCTC 2 cut(s) 910, 1189
BcuI ACTAGT 1 cut(s) 511
BfaI CTAG 4 cut(s) 512, 545, 660, 840
BfmI CTRYAG 4 cut(s) 240, 378, 895, 955
BfuAI ACCTGC 1 cut(s) 133
BlnI CCTAGG 1 cut(s) 659
BmcAI AGTACT 1 cut(s) 1381
Bme1390I CCNGG 1 cut(s) 948
BmeT110I CYCGRG 2 cut(s) 606, 612
BmiI GGNNCC 4 cut(s) 952, 1035, 1288, 1372
BmrFI CCNGG 1 cut(s) 948
BmrI ACTGGG 1 cut(s) 1000
BmsI GCATC 3 cut(s) 343, 1174, 1251
BmuI ACTGGG 1 cut(s) 1000
BplI GAGNNNNNCTC 2 cut(s) 1355, 1387
BpmI CTGGAG 2 cut(s) 1164, 1357
Bpu10I CCTNAGC 1 cut(s) 701
BsaBI GATNNNNATC 1 cut(s) 144
BsaJI CCNNGG 6 cut(s) 318, 336, 659, 1061, 1247, 1313
BsaWI WCCGGW 1 cut(s) 1030
BsaXI ACNNNNNCTCC 2 cut(s) 175, 205
Bsc4I CCNNNNNNNGG 4 cut(s) 573, 671, 678, 970
Bse118I RCCGGY 1 cut(s) 671
Bse1I ACTGG 2 cut(s) 1006, 1306
Bse3DI GCAATG 1 cut(s) 907
Bse8I GATNNNNATC 1 cut(s) 144
BseBI CCWGG 1 cut(s) 948
BseDI CCNNGG 6 cut(s) 318, 336, 659, 1061, 1247, 1313
BseGI GGATG 3 cut(s) 212, 636, 665
BseJI GATNNNNATC 1 cut(s) 144
BseLI CCNNNNNNNGG 4 cut(s) 573, 671, 678, 970
BseMI GCAATG 1 cut(s) 907
BseMII CTCAG 5 cut(s) 632, 715, 866, 944, 1356
BseNI ACTGG 2 cut(s) 1006, 1306
BseRI GAGGAG 1 cut(s) 196
BseSI GKGCMC 2 cut(s) 273, 1165
BseX3I CGGCCG 1 cut(s) 441
BseYI CCCAGC 1 cut(s) 217
BsgI GTGCAG 2 cut(s) 354, 843
Bsh1236I CGCG 1 cut(s) 1213
Bsh1285I CGRYCG 3 cut(s) 444, 829, 1211
BshFI GGCC 7 cut(s) 443, 560, 664, 963, 1066, 1121, 1142
BsiEI CGRYCG 3 cut(s) 444, 829, 1211
BsiHKAI GWGCWC 5 cut(s) 273, 613, 619, 1165, 1389
BsiHKCI CYCGRG 2 cut(s) 606, 612
BsiSI CCGG 2 cut(s) 672, 1031
BslI CCNNNNNNNGG 4 cut(s) 573, 671, 678, 970
BsmAI GTCTC 2 cut(s) 910, 1189
BsmI GAATGC 1 cut(s) 706
BsnI GGCC 7 cut(s) 443, 560, 664, 963, 1066, 1121, 1142
BsoBI CYCGRG 2 cut(s) 606, 612
Bsp1286I GDGCHC 5 cut(s) 273, 613, 619, 1165, 1389
Bsp143I GATC 7 cut(s) 55, 139, 763, 826, 1033, 1151, 1337
BspACI CCGC 8 cut(s) 360, 394, 426, 440, 444, 574, 1211, 1293
BspANI GGCC 7 cut(s) 443, 560, 664, 963, 1066, 1121, 1142
BspCNI CTCAG 5 cut(s) 631, 714, 865, 943, 1355
BspFNI CGCG 1 cut(s) 1213
BspLI GGNNCC 4 cut(s) 952, 1035, 1288, 1372
BspMAI CTGCAG 3 cut(s) 382, 899, 959
BspMI ACCTGC 1 cut(s) 133
BspPI GGATC 4 cut(s) 771, 1028, 1041, 1332
BsrBI CCGCTC 1 cut(s) 1295
BsrDI GCAATG 1 cut(s) 907
BsrFI RCCGGY 1 cut(s) 671
BsrI ACTGG 2 cut(s) 1006, 1306
BssAI RCCGGY 1 cut(s) 671
BssECI CCNNGG 6 cut(s) 318, 336, 659, 1061, 1247, 1313
BssMI GATC 7 cut(s) 55, 139, 763, 826, 1033, 1151, 1337
BssT1I CCWWGG 4 cut(s) 318, 659, 1247, 1313
Bst2UI CCWGG 1 cut(s) 948
Bst4CI ACNGT 2 cut(s) 652, 1384
Bst6I CTCTTC 3 cut(s) 173, 429, 732
BstAPI GCANNNNNTGC 3 cut(s) 368, 377, 497
BstC8I GCNNGC 4 cut(s) 525, 581, 585, 955
BstDEI CTNAG 6 cut(s) 23, 618, 701, 852, 930, 1342
BstDSI CCRYGG 1 cut(s) 336
BstEII GGTNACC 1 cut(s) 539
BstF5I GGATG 3 cut(s) 212, 636, 665
BstFNI CGCG 1 cut(s) 1213
BstKTI GATC 7 cut(s) 58, 142, 766, 829, 1036, 1154, 1340
BstMAI GTCTC 2 cut(s) 910, 1189
BstMBI GATC 7 cut(s) 55, 139, 763, 826, 1033, 1151, 1337
BstMCI CGRYCG 3 cut(s) 444, 829, 1211
BstNI CCWGG 1 cut(s) 948
BstNSI RCATGY 3 cut(s) 317, 583, 1283
BstPI GGTNACC 1 cut(s) 539
BstSCI CCNGG 1 cut(s) 946
BstSFI CTRYAG 4 cut(s) 240, 378, 895, 955
BstSLI GKGCMC 2 cut(s) 273, 1165
BstUI CGCG 1 cut(s) 1213
BstX2I RGATCY 3 cut(s) 763, 1033, 1337
BstYI RGATCY 3 cut(s) 763, 1033, 1337
BstZI CGGCCG 1 cut(s) 441
BsuRI GGCC 7 cut(s) 443, 560, 664, 963, 1066, 1121, 1142
BtgI CCRYGG 1 cut(s) 336
BtsCI GGATG 3 cut(s) 212, 636, 665
BtsI GCAGTG 2 cut(s) 330, 964
BtsIMutI CAGTG 3 cut(s) 330, 964, 1013
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 4 cut(s) 525, 581, 585, 955
CaiI CAGNNNCTG 3 cut(s) 368, 386, 407
CciNI GCGGCCGC 1 cut(s) 441
Cfr10I RCCGGY 1 cut(s) 671
Csp6I GTAC 4 cut(s) 1105, 1309, 1380, 1447
CviAII CATG 8 cut(s) 314, 391, 580, 733, 937, 1117, 1280, 1436
CviQI GTAC 4 cut(s) 1105, 1309, 1380, 1447
DdeI CTNAG 6 cut(s) 23, 618, 701, 852, 930, 1342
DpnI GATC 7 cut(s) 57, 141, 765, 828, 1035, 1153, 1339
DpnII GATC 7 cut(s) 55, 139, 763, 826, 1033, 1151, 1337
DraIII CACNNNGTG 2 cut(s) 547, 1160
EaeI YGGCCR 3 cut(s) 441, 961, 1119
EagI CGGCCG 1 cut(s) 441
Eam1104I CTCTTC 3 cut(s) 173, 429, 732
EarI CTCTTC 3 cut(s) 173, 429, 732
Ecl136II GAGCTC 2 cut(s) 611, 617
EclXI CGGCCG 1 cut(s) 441
Eco130I CCWWGG 4 cut(s) 318, 659, 1247, 1313
Eco24I GRGCYC 2 cut(s) 613, 619
Eco52I CGGCCG 1 cut(s) 441
Eco53kI GAGCTC 2 cut(s) 611, 617
Eco88I CYCGRG 2 cut(s) 606, 612
Eco91I GGTNACC 1 cut(s) 539
EcoICRI GAGCTC 2 cut(s) 611, 617
EcoO65I GGTNACC 1 cut(s) 539
EcoRII CCWGG 1 cut(s) 946
EcoT14I CCWWGG 4 cut(s) 318, 659, 1247, 1313
EcoT22I ATGCAT 1 cut(s) 1189
EcoT38I GRGCYC 2 cut(s) 613, 619
ErhI CCWWGG 4 cut(s) 318, 659, 1247, 1313
FaeI CATG 8 cut(s) 317, 394, 583, 736, 940, 1120, 1283, 1439
FalI AAGNNNNNCTT 2 cut(s) 1033, 1065
FatI CATG 8 cut(s) 313, 390, 579, 732, 936, 1116, 1279, 1435
FokI GGATG 3 cut(s) 199, 643, 652
FriOI GRGCYC 2 cut(s) 613, 619
FspBI CTAG 4 cut(s) 512, 545, 660, 840
GsaI CCCAGC 1 cut(s) 221
GsuI CTGGAG 2 cut(s) 1164, 1357
HaeIII GGCC 7 cut(s) 443, 560, 664, 963, 1066, 1121, 1142
HapII CCGG 2 cut(s) 672, 1031
Hin1II CATG 8 cut(s) 317, 394, 583, 736, 940, 1120, 1283, 1439
HindIII AAGCTT 1 cut(s) 28
HpaII CCGG 2 cut(s) 672, 1031
HphI GGTGA 5 cut(s) 114, 533, 604, 900, 1381
Hpy166II GTNNAC 2 cut(s) 271, 1163
Hpy188I TCNGA 9 cut(s) 114, 144, 197, 292, 621, 630, 927, 986, 1062
Hpy188III TCNNGA 6 cut(s) 34, 137, 840, 932, 1041, 1181
Hpy8I GTNNAC 2 cut(s) 271, 1163
HpyAV CCTTC 7 cut(s) 192, 672, 784, 991, 1209, 1223, 1227
HpyCH4III ACNGT 2 cut(s) 652, 1384
HpyF3I CTNAG 6 cut(s) 23, 618, 701, 852, 930, 1342
Hsp92II CATG 8 cut(s) 317, 394, 583, 736, 940, 1120, 1283, 1439
Kzo9I GATC 7 cut(s) 55, 139, 763, 826, 1033, 1151, 1337
LmnI GCTCC 3 cut(s) 919, 950, 1376
LweI GCATC 3 cut(s) 343, 1174, 1251
MaeI CTAG 4 cut(s) 512, 545, 660, 840
MaeIII GTNAC 2 cut(s) 539, 592
MalI GATC 7 cut(s) 57, 141, 765, 828, 1035, 1153, 1339
MbiI CCGCTC 1 cut(s) 1295
MboI GATC 7 cut(s) 55, 139, 763, 826, 1033, 1151, 1337
MboII GAAGA 7 cut(s) 190, 446, 650, 749, 1125, 1266, 1270
MfeI CAATTG 1 cut(s) 1082
MflI RGATCY 3 cut(s) 763, 1033, 1337
MhlI GDGCHC 5 cut(s) 273, 613, 619, 1165, 1389
MlsI TGGCCA 2 cut(s) 963, 1121
MluCI AATT 9 cut(s) 115, 160, 295, 453, 719, 1020, 1082, 1111, 1300
MluNI TGGCCA 2 cut(s) 963, 1121
MlyI GAGTC 5 cut(s) 224, 830, 844, 922, 952
MmeI TCCRAC 1 cut(s) 104
Mox20I TGGCCA 2 cut(s) 963, 1121
Mph1103I ATGCAT 1 cut(s) 1189
MscI TGGCCA 2 cut(s) 963, 1121
MseI TTAA 3 cut(s) 654, 747, 1428
Msp20I TGGCCA 2 cut(s) 963, 1121
MspA1I CMGCKG 3 cut(s) 365, 383, 407
MspI CCGG 2 cut(s) 672, 1031
MspR9I CCNGG 1 cut(s) 948
MunI CAATTG 1 cut(s) 1082
Mva1269I GAATGC 1 cut(s) 706
MvaI CCWGG 1 cut(s) 948
MvnI CGCG 1 cut(s) 1213
NdeII GATC 7 cut(s) 55, 139, 763, 826, 1033, 1151, 1337
NlaIII CATG 8 cut(s) 317, 394, 583, 736, 940, 1120, 1283, 1439
NlaIV GGNNCC 4 cut(s) 952, 1035, 1288, 1372
NmuCI GTSAC 2 cut(s) 539, 592
NotI GCGGCCGC 1 cut(s) 441
NsiI ATGCAT 1 cut(s) 1189
NspI RCATGY 3 cut(s) 317, 583, 1283
PaeI GCATGC 1 cut(s) 583
PaeR7I CTCGAG 2 cut(s) 606, 612
PaqCI CACCTGC 1 cut(s) 133
PciI ACATGT 1 cut(s) 1279
PctI GAATGC 1 cut(s) 706
PfeI GAWTC 5 cut(s) 145, 625, 773, 811, 1237
PflFI GACNNNGTC 1 cut(s) 853
PfoI TCCNGGA 1 cut(s) 946
Ple19I CGATCG 1 cut(s) 829
PleI GAGTC 5 cut(s) 224, 830, 844, 922, 951
PpsI GAGTC 5 cut(s) 224, 830, 844, 922, 951
PscI ACATGT 1 cut(s) 1279
PsiI TTATAA 1 cut(s) 714
Psp124BI GAGCTC 2 cut(s) 613, 619
Psp6I CCWGG 1 cut(s) 946
PspEI GGTNACC 1 cut(s) 539
PspFI CCCAGC 1 cut(s) 217
PspGI CCWGG 1 cut(s) 946
PspN4I GGNNCC 4 cut(s) 952, 1035, 1288, 1372
PspXI VCTCGAGB 2 cut(s) 606, 612
PstI CTGCAG 3 cut(s) 382, 899, 959
PstNI CAGNNNCTG 3 cut(s) 368, 386, 407
PsuI RGATCY 3 cut(s) 763, 1033, 1337
PsyI GACNNNGTC 1 cut(s) 853
PvuI CGATCG 1 cut(s) 829
PvuII CAGCTG 3 cut(s) 365, 383, 407
RsaI GTAC 4 cut(s) 1106, 1310, 1381, 1448
RsaNI GTAC 4 cut(s) 1105, 1309, 1380, 1447
SacI GAGCTC 2 cut(s) 613, 619
SaqAI TTAA 3 cut(s) 654, 747, 1428
Sau3AI GATC 7 cut(s) 55, 139, 763, 826, 1033, 1151, 1337
ScaI AGTACT 1 cut(s) 1381
SchI GAGTC 5 cut(s) 224, 830, 844, 922, 952
ScrFI CCNGG 1 cut(s) 948
SduI GDGCHC 5 cut(s) 273, 613, 619, 1165, 1389
SfaNI GCATC 3 cut(s) 343, 1174, 1251
SfcI CTRYAG 4 cut(s) 240, 378, 895, 955
Sfr274I CTCGAG 2 cut(s) 606, 612
SlaI CTCGAG 2 cut(s) 606, 612
SmlI CTYRAG 2 cut(s) 606, 612
SmoI CTYRAG 2 cut(s) 606, 612
SpeI ACTAGT 1 cut(s) 511
SphI GCATGC 1 cut(s) 583
Sse9I AATT 9 cut(s) 115, 160, 295, 453, 719, 1020, 1082, 1111, 1300
SsiI CCGC 8 cut(s) 360, 394, 426, 440, 444, 574, 1211, 1293
SspMI CTAG 4 cut(s) 512, 545, 660, 840
SstI GAGCTC 2 cut(s) 613, 619
StyD4I CCNGG 1 cut(s) 946
StyI CCWWGG 4 cut(s) 318, 659, 1247, 1313
TaaI ACNGT 2 cut(s) 652, 1384
TaqI TCGA 6 cut(s) 607, 613, 809, 829, 848, 969
TasI AATT 9 cut(s) 115, 160, 295, 453, 719, 1020, 1082, 1111, 1300
TatI WGTACW 1 cut(s) 1379
TauI GCSGC 4 cut(s) 397, 428, 443, 446
TfiI GAWTC 5 cut(s) 145, 625, 773, 811, 1237
Tru1I TTAA 3 cut(s) 654, 747, 1428
Tru9I TTAA 3 cut(s) 654, 747, 1428
TscAI CASTG 3 cut(s) 330, 964, 1013
TseFI GTSAC 2 cut(s) 539, 592
Tsp45I GTSAC 2 cut(s) 539, 592
TspDTI ATGAA 1 cut(s) 732
TspGWI ACGGA 1 cut(s) 451
TspRI CASTG 3 cut(s) 330, 964, 1013
Tth111I GACNNNGTC 1 cut(s) 853
VneI GTGCAC 2 cut(s) 269, 1161
XapI RAATTY 3 cut(s) 160, 453, 719
XbaI TCTAGA 1 cut(s) 839
XceI RCATGY 3 cut(s) 317, 583, 1283
XhoI CTCGAG 2 cut(s) 606, 612
XmaJI CCTAGG 1 cut(s) 659
XspI CTAG 4 cut(s) 512, 545, 660, 840
ZrmI AGTACT 1 cut(s) 1381
Zsp2I ATGCAT 1 cut(s) 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.