pycom16g16730

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010897
Physical Location & Seq
Reverse (-)
12621753 .. 12622909
1157 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g16730.3

Sequence Viewer

Length: 861 bp
ATGCAAGCATTGGTGACCGCTCAGGCTCGAGCTCGAGCTCAGAGGATCCGGATGGCCGAAGACAAAAATACTGTCAATCCTAGACATTCCACCCCCAGAAGATCTTTACAGGAAAACCGGTCCAGGCATACGTATAACGAAATGGACAGAGGAATTGAAGAGAACATTAAGACTGTGGAGATGGATCTTGGTGAATCAAAAGGAATTTTGAAGAGCAGAAACAGCAGCTATTCAAATCACTCGAAAACAGAGAGAACAGAACAACATAGATTTTCAACTCAGTATGCACCAAATCAAGCATACTCAAACCAAGAAACCTACCAGCAGGCCTCTCTGGCTCCATCAACATTGACTCATAGAAGCCCGGGAGCCTGCAGTGCACATTTTGAGGACTACTCTTTTGGAACAGCTCAAAGCAGCCCTCAGTGCTATTCTGCCATGTCCAAAATTGATCCCTCAAAAGCTCCCTTCGCCTTCCCTAGGCCGGATTATGGAGAGCCCTTGTCCTATGACTACCCTTGGTTCCCAAACTACATGGCCAACACACAATCTTCAAAGGCGAAAGCCCGATCACAGAGTGCACCGAAGCAAAGTCCTATTGATACAGTTGAGGGGCCGCCAAGGCGGCAGCGGAGGGCGTCAATGGAGGGAATGAAAATCCCAAGGGCTGTGACGATGCAGAGGTCATCTTCTCATGTGAGTTCAACTGCTCAGGGTTATCAGTACCCTTCGTATATCAAGCTTGATAGGTCCACAGTTTCACTCAAAGAAAGTGAGTGTGGCTCCGGCAGTACAGTGCTCACAAACACCGATTACTTCAGATCTCTTGTTGCATATGATGCACATAGAAATAGTTACTAA

Protein Analysis

287

Amino Acids

32.3

Weight (kDa)

9.53

Isoelectric Point (pI)

66.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010897)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14750 AT4G14750 AT4G14750
fragaria_vesca FvH4_4g01000 FvH4_4g01000
malus_domestica MD13G1198300.v1.1 MD16G1198400.v1.1
prunus_persica Prupe.1G020000_v2.0.a1
pyrus_communis pycom13g17210 pycom16g16730
rosa_chinensis RchiOBHm_Chr4g0387551
rosa_laevigata RLG00000010112
rosa_multiflora Rmu_sc0007248.1_g000016
rosa_roxburghii Rroxscaffold_5G00334230
rosa_rugosa Rorug03G0310600
rosa_samantha Rh4AG016600 Rh4BG011500 Rh4CG017800 Rh4DG012400
rosa_wichuraiana Rw4G001060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 20
AccIII TCCGGA 1 cut(s) 48
AciI CCGC 4 cut(s) 18, 617, 625, 631
AclWI GGATC 4 cut(s) 40, 53, 192, 446
AcoI YGGCCR 2 cut(s) 54, 537
AcsI RAATTY 1 cut(s) 204
AcuI CTGAAG 1 cut(s) 802
AcyI GRCGYC 1 cut(s) 638
AdeI CACNNNGTG 1 cut(s) 578
AfaI GTAC 2 cut(s) 725, 793
AfiI CCNNNNNNNGG 3 cut(s) 480, 484, 491
AgeI ACCGGT 1 cut(s) 117
AgsI TTSAA 6 cut(s) 158, 211, 234, 276, 555, 705
AjnI CCWGG 1 cut(s) 122
AluBI AGCT 6 cut(s) 32, 38, 228, 410, 464, 742
AluI AGCT 6 cut(s) 32, 38, 228, 410, 464, 742
Alw21I GWGCWC 5 cut(s) 34, 40, 382, 583, 801
Alw44I GTGCAC 2 cut(s) 378, 579
AlwI GGATC 4 cut(s) 40, 53, 192, 446
Ama87I CYCGRG 3 cut(s) 27, 33, 364
Aor13HI TCCGGA 1 cut(s) 48
AoxI GGCC 5 cut(s) 54, 327, 482, 537, 614
ApaLI GTGCAC 2 cut(s) 378, 579
ApeKI GCWGC 3 cut(s) 225, 417, 628
ApoI RAATTY 1 cut(s) 204
ArsI GACNNNNNNTTYG 4 cut(s) 57, 89, 383, 415
AsiGI ACCGGT 1 cut(s) 117
AspA2I CCTAGG 1 cut(s) 479
AspS9I GGNCC 3 cut(s) 120, 614, 750
AsuC2I CCSGG 2 cut(s) 365, 366
AsuHPI GGTGA 2 cut(s) 25, 203
AvaI CYCGRG 3 cut(s) 27, 33, 364
AvaII GGWCC 2 cut(s) 120, 750
AvrII CCTAGG 1 cut(s) 479
BaeGI GKGCMC 2 cut(s) 382, 583
BalI TGGCCA 1 cut(s) 539
BamHI GGATCC 1 cut(s) 45
BanII GRGCYC 3 cut(s) 34, 40, 501
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 5 cut(s) 34, 40, 382, 583, 801
BbvI GCAGC 3 cut(s) 237, 429, 640
BccI CCATC 3 cut(s) 46, 175, 349
BciT130I CCWGG 1 cut(s) 124
BcnI CCSGG 2 cut(s) 365, 366
BfaI CTAG 2 cut(s) 81, 480
BfmI CTRYAG 1 cut(s) 373
BglI GCCNNNNNGGC 3 cut(s) 335, 622, 625
BglII AGATCT 2 cut(s) 101, 821
BisI GCNGC 5 cut(s) 226, 418, 617, 626, 629
BlnI CCTAGG 1 cut(s) 479
BlsI GCNGC 5 cut(s) 227, 419, 618, 627, 630
Bme1390I CCNGG 3 cut(s) 124, 365, 366
Bme18I GGWCC 2 cut(s) 120, 750
BmeT110I CYCGRG 3 cut(s) 27, 33, 364
BmgT120I GGNCC 3 cut(s) 120, 614, 750
BmiI GGNNCC 6 cut(s) 47, 339, 370, 524, 615, 784
BmrFI CCNGG 3 cut(s) 124, 365, 366
BmsI GCATC 2 cut(s) 666, 829
BpiI GAAGAC 1 cut(s) 66
BplI GAGNNNNNCTC 4 cut(s) 380, 412, 767, 799
Bpu10I CCTNAGC 2 cut(s) 21, 711
BpuMI CCSGG 2 cut(s) 365, 366
BsaAI YACGTR 1 cut(s) 132
BsaHI GRCGYC 1 cut(s) 638
BsaJI CCNNGG 5 cut(s) 364, 479, 518, 620, 662
BsaWI WCCGGW 2 cut(s) 48, 117
Bsc4I CCNNNNNNNGG 3 cut(s) 480, 484, 491
Bse118I RCCGGY 1 cut(s) 117
BseAI TCCGGA 1 cut(s) 48
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 5 cut(s) 364, 479, 518, 620, 662
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 3 cut(s) 480, 484, 491
BseMII CTCAG 5 cut(s) 35, 53, 293, 437, 725
BseSI GKGCMC 2 cut(s) 382, 583
BseXI GCAGC 3 cut(s) 237, 429, 640
BshFI GGCC 5 cut(s) 56, 329, 484, 539, 616
BshTI ACCGGT 1 cut(s) 117
BsiHKAI GWGCWC 5 cut(s) 34, 40, 382, 583, 801
BsiHKCI CYCGRG 3 cut(s) 27, 33, 364
BsiSI CCGG 5 cut(s) 49, 118, 365, 485, 786
BslI CCNNNNNNNGG 3 cut(s) 480, 484, 491
BsnI GGCC 5 cut(s) 56, 329, 484, 539, 616
BsoBI CYCGRG 3 cut(s) 27, 33, 364
Bsp1286I GDGCHC 6 cut(s) 34, 40, 382, 501, 583, 801
Bsp13I TCCGGA 1 cut(s) 48
Bsp143I GATC 6 cut(s) 45, 101, 184, 451, 569, 821
BspACI CCGC 4 cut(s) 18, 617, 625, 631
BspANI GGCC 5 cut(s) 56, 329, 484, 539, 616
BspCNI CTCAG 5 cut(s) 34, 52, 292, 436, 724
BspEI TCCGGA 1 cut(s) 48
BspLI GGNNCC 6 cut(s) 47, 339, 370, 524, 615, 784
BspMAI CTGCAG 1 cut(s) 377
BspPI GGATC 4 cut(s) 40, 53, 192, 446
BspQI GCTCTTC 1 cut(s) 206
BsrBI CCGCTC 1 cut(s) 20
BsrFI RCCGGY 1 cut(s) 117
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 5 cut(s) 364, 479, 518, 620, 662
BssMI GATC 6 cut(s) 45, 101, 184, 451, 569, 821
BssNI GRCGYC 1 cut(s) 638
BssT1I CCWWGG 4 cut(s) 479, 518, 620, 662
Bst2UI CCWGG 1 cut(s) 124
Bst4CI ACNGT 5 cut(s) 73, 175, 607, 757, 796
Bst6I CTCTTC 2 cut(s) 153, 206
BstACI GRCGYC 1 cut(s) 638
BstAPI GCANNNNNTGC 1 cut(s) 839
BstBAI YACGTR 1 cut(s) 132
BstC8I GCNNGC 3 cut(s) 6, 327, 373
BstDEI CTNAG 5 cut(s) 21, 39, 279, 423, 711
BstEII GGTNACC 1 cut(s) 13
BstENI CCTNNNNNAGG 1 cut(s) 478
BstF5I GGATG 1 cut(s) 57
BstKTI GATC 6 cut(s) 48, 104, 187, 454, 572, 824
BstMBI GATC 6 cut(s) 45, 101, 184, 451, 569, 821
BstMWI GCNNNNNNNGC 8 cut(s) 222, 335, 377, 426, 470, 622, 625, 839
BstNI CCWGG 1 cut(s) 124
BstPI GGTNACC 1 cut(s) 13
BstSCI CCNGG 3 cut(s) 122, 363, 364
BstSFI CTRYAG 1 cut(s) 373
BstSLI GKGCMC 2 cut(s) 382, 583
BstSNI TACGTA 1 cut(s) 132
BstV1I GCAGC 3 cut(s) 237, 429, 640
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 4 cut(s) 45, 101, 184, 821
BstYI RGATCY 4 cut(s) 45, 101, 184, 821
BsuRI GGCC 5 cut(s) 56, 329, 484, 539, 616
BtsCI GGATG 1 cut(s) 57
BtsI GCAGTG 1 cut(s) 382
BtsIMutI CAGTG 3 cut(s) 382, 431, 801
Cac8I GCNNGC 3 cut(s) 6, 327, 373
Cfr10I RCCGGY 1 cut(s) 117
Cfr13I GGNCC 3 cut(s) 120, 614, 750
Cfr9I CCCGGG 1 cut(s) 364
CseI GACGC 1 cut(s) 627
Csp6I GTAC 2 cut(s) 724, 792
CspAI ACCGGT 1 cut(s) 117
CviAII CATG 3 cut(s) 439, 535, 695
CviQI GTAC 2 cut(s) 724, 792
DdeI CTNAG 5 cut(s) 21, 39, 279, 423, 711
DpnI GATC 6 cut(s) 47, 103, 186, 453, 571, 823
DpnII GATC 6 cut(s) 45, 101, 184, 451, 569, 821
DraIII CACNNNGTG 1 cut(s) 578
EaeI YGGCCR 2 cut(s) 54, 537
Eam1104I CTCTTC 2 cut(s) 153, 206
EarI CTCTTC 2 cut(s) 153, 206
Ecl136II GAGCTC 2 cut(s) 32, 38
Eco105I TACGTA 1 cut(s) 132
Eco130I CCWWGG 4 cut(s) 479, 518, 620, 662
Eco147I AGGCCT 1 cut(s) 329
Eco24I GRGCYC 3 cut(s) 34, 40, 501
Eco47I GGWCC 2 cut(s) 120, 750
Eco53kI GAGCTC 2 cut(s) 32, 38
Eco57I CTGAAG 1 cut(s) 802
Eco88I CYCGRG 3 cut(s) 27, 33, 364
Eco91I GGTNACC 1 cut(s) 13
EcoICRI GAGCTC 2 cut(s) 32, 38
EcoNI CCTNNNNNAGG 1 cut(s) 478
EcoO65I GGTNACC 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 122
EcoT14I CCWWGG 4 cut(s) 479, 518, 620, 662
EcoT38I GRGCYC 3 cut(s) 34, 40, 501
ErhI CCWWGG 4 cut(s) 479, 518, 620, 662
FaeI CATG 3 cut(s) 442, 538, 698
FatI CATG 3 cut(s) 438, 534, 694
FauNDI CATATG 1 cut(s) 835
Fnu4HI GCNGC 5 cut(s) 226, 418, 617, 626, 629
FokI GGATG 1 cut(s) 64
FriOI GRGCYC 3 cut(s) 34, 40, 501
Fsp4HI GCNGC 5 cut(s) 226, 418, 617, 626, 629
FspBI CTAG 2 cut(s) 81, 480
GluI GCNGC 5 cut(s) 226, 418, 617, 626, 629
HaeIII GGCC 5 cut(s) 56, 329, 484, 539, 616
HapII CCGG 5 cut(s) 49, 118, 365, 485, 786
HgaI GACGC 1 cut(s) 627
Hin1I GRCGYC 1 cut(s) 638
Hin1II CATG 3 cut(s) 442, 538, 698
HindIII AAGCTT 1 cut(s) 740
HinfI GANTC 2 cut(s) 194, 352
HpaII CCGG 5 cut(s) 49, 118, 365, 485, 786
HphI GGTGA 2 cut(s) 25, 203
Hpy166II GTNNAC 3 cut(s) 380, 581, 753
Hpy188I TCNGA 2 cut(s) 42, 821
Hpy188III TCNNGA 1 cut(s) 49
Hpy8I GTNNAC 3 cut(s) 380, 581, 753
HpyAV CCTTC 3 cut(s) 478, 484, 738
HpyCH4III ACNGT 5 cut(s) 73, 175, 607, 757, 796
HpyCH4IV ACGT 1 cut(s) 131
HpyCH4V TGCA 8 cut(s) 4, 287, 375, 380, 581, 679, 833, 842
HpyF10VI GCNNNNNNNGC 8 cut(s) 222, 335, 377, 426, 470, 622, 625, 839
HpyF3I CTNAG 5 cut(s) 21, 39, 279, 423, 711
HpySE526I ACGT 1 cut(s) 131
Hsp92I GRCGYC 1 cut(s) 638
Hsp92II CATG 3 cut(s) 442, 538, 698
Kpn2I TCCGGA 1 cut(s) 48
Kzo9I GATC 6 cut(s) 45, 101, 184, 451, 569, 821
LguI GCTCTTC 1 cut(s) 206
LmnI GCTCC 4 cut(s) 343, 368, 469, 788
Lsp1109I GCAGC 3 cut(s) 237, 429, 640
LweI GCATC 2 cut(s) 666, 829
MaeI CTAG 2 cut(s) 81, 480
MaeII ACGT 1 cut(s) 131
MaeIII GTNAC 3 cut(s) 13, 670, 854
MalI GATC 6 cut(s) 47, 103, 186, 453, 571, 823
MbiI CCGCTC 1 cut(s) 20
MboI GATC 6 cut(s) 45, 101, 184, 451, 569, 821
MboII GAAGA 6 cut(s) 71, 111, 170, 223, 543, 681
MflI RGATCY 4 cut(s) 45, 101, 184, 821
MhlI GDGCHC 6 cut(s) 34, 40, 382, 501, 583, 801
MlsI TGGCCA 1 cut(s) 539
MluCI AATT 3 cut(s) 153, 204, 447
MluNI TGGCCA 1 cut(s) 539
MlyI GAGTC 1 cut(s) 346
Mox20I TGGCCA 1 cut(s) 539
MroI TCCGGA 1 cut(s) 48
MscI TGGCCA 1 cut(s) 539
MseI TTAA 1 cut(s) 168
Msp20I TGGCCA 1 cut(s) 539
MspA1I CMGCKG 1 cut(s) 631
MspI CCGG 5 cut(s) 49, 118, 365, 485, 786
MspR9I CCNGG 3 cut(s) 124, 365, 366
MvaI CCWGG 1 cut(s) 124
MwoI GCNNNNNNNGC 8 cut(s) 222, 335, 377, 426, 470, 622, 625, 839
NciI CCSGG 2 cut(s) 365, 366
NdeI CATATG 1 cut(s) 835
NdeII GATC 6 cut(s) 45, 101, 184, 451, 569, 821
NlaIII CATG 3 cut(s) 442, 538, 698
NlaIV GGNNCC 6 cut(s) 47, 339, 370, 524, 615, 784
NmuCI GTSAC 2 cut(s) 13, 670
PaeR7I CTCGAG 2 cut(s) 27, 33
PceI AGGCCT 1 cut(s) 329
PciSI GCTCTTC 1 cut(s) 206
PfeI GAWTC 1 cut(s) 194
PinAI ACCGGT 1 cut(s) 117
PkrI GCNGC 5 cut(s) 227, 419, 618, 627, 630
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
Ppu21I YACGTR 1 cut(s) 132
Psp124BI GAGCTC 2 cut(s) 34, 40
Psp6I CCWGG 1 cut(s) 122
PspEI GGTNACC 1 cut(s) 13
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 6 cut(s) 47, 339, 370, 524, 615, 784
PspPI GGNCC 3 cut(s) 120, 614, 750
PspXI VCTCGAGB 2 cut(s) 27, 33
PstI CTGCAG 1 cut(s) 377
PsuI RGATCY 4 cut(s) 45, 101, 184, 821
RsaI GTAC 2 cut(s) 725, 793
RsaNI GTAC 2 cut(s) 724, 792
SacI GAGCTC 2 cut(s) 34, 40
SapI GCTCTTC 1 cut(s) 206
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 5 cut(s) 226, 418, 617, 626, 629
Sau3AI GATC 6 cut(s) 45, 101, 184, 451, 569, 821
Sau96I GGNCC 3 cut(s) 120, 614, 750
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 3 cut(s) 124, 365, 366
SduI GDGCHC 6 cut(s) 34, 40, 382, 501, 583, 801
SfaNI GCATC 2 cut(s) 666, 829
SfcI CTRYAG 1 cut(s) 373
Sfr274I CTCGAG 2 cut(s) 27, 33
SinI GGWCC 2 cut(s) 120, 750
SlaI CTCGAG 2 cut(s) 27, 33
SmaI CCCGGG 1 cut(s) 366
SmlI CTYRAG 2 cut(s) 27, 33
SmoI CTYRAG 2 cut(s) 27, 33
SnaBI TACGTA 1 cut(s) 132
Sse9I AATT 3 cut(s) 153, 204, 447
SseBI AGGCCT 1 cut(s) 329
SsiI CCGC 4 cut(s) 18, 617, 625, 631
SspMI CTAG 2 cut(s) 81, 480
SstI GAGCTC 2 cut(s) 34, 40
StuI AGGCCT 1 cut(s) 329
StyD4I CCNGG 3 cut(s) 122, 363, 364
StyI CCWWGG 4 cut(s) 479, 518, 620, 662
TaaI ACNGT 5 cut(s) 73, 175, 607, 757, 796
TaiI ACGT 1 cut(s) 134
TaqI TCGA 3 cut(s) 28, 34, 242
TasI AATT 3 cut(s) 153, 204, 447
TatI WGTACW 1 cut(s) 791
TauI GCSGC 2 cut(s) 619, 628
TfiI GAWTC 1 cut(s) 194
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 3 cut(s) 382, 431, 801
TseFI GTSAC 2 cut(s) 13, 670
TseI GCWGC 3 cut(s) 225, 417, 628
Tsp45I GTSAC 2 cut(s) 13, 670
TspDTI ATGAA 1 cut(s) 668
TspMI CCCGGG 1 cut(s) 364
TspRI CASTG 3 cut(s) 382, 431, 801
VneI GTGCAC 2 cut(s) 378, 579
VpaK11BI GGWCC 2 cut(s) 120, 750
XagI CCTNNNNNAGG 1 cut(s) 478
XapI RAATTY 1 cut(s) 204
XhoI CTCGAG 2 cut(s) 27, 33
XmaI CCCGGG 1 cut(s) 364
XmaJI CCTAGG 1 cut(s) 479
XspI CTAG 2 cut(s) 81, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.