AT4G15720

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016096
Physical Location & Seq
Forward (+)
8949460 .. 8951540
2081 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G15720.1

Sequence Viewer

Length: 1851 bp
ATGAAGAAAGGGTTCATCCAAAATGTCCATTTAGCCCCTGCAACTTCTCTGTTCGTCCCTCAGTACAAAAACGACTTCTTTCATCTCAAAACCAAAGCTTTCTTGGTTCACAAGCTTTCAGAATCTACCAACGCTGCTTTCACGAATTTACTACATACTCTGACTCTGAAACTTGGGTTCGCGAGCGACACTTTCACTGTGAATCACCTAGTTATTAGTTATGTCAAGCTCAAAGAAATCAACACTGCACGCAAACTGTTTGATGAAATGTGTGAACCAAATGTGGTTTCATGGACTTCGGTTATTTCTGGGTATAACGACATGGGTAAACCTCAAAATGCTCTTTCTATGTTTCAGAAGATGCATGAAGATAGACCTGTGCCTCCCAATGAGTACACGTTTGCTAGTGTCTTTAAAGCATGTTCTGCTTTGGCTGAATCAAGAATCGGTAAAAACATCCATGCCCGTCTGGAGATTTCAGGGCTCAGAAGGAATATTGTAGTGAGTTCCTCTCTTGTTGACATGTATGGGAAATGCAACGATGTGGAAACCGCTAGACGGGTTTTCGACTCGATGATTGGTTATGGAAGAAATGTTGTTTCTTGGACCTCGATGATCACTGCTTATGCGCAGAACGCTCGTGGCCATGAAGCTATTGAGTTGTTTAGATCATTCAATGCTGCTTTGACGTCGGATAGGGCGAATCAGTTTATGCTGGCTAGTGTAATTAGCGCATGCTCTAGTTTAGGTAGGCTGCAATGGGGGAAAGTTGCTCACGGGTTAGTTACTCGAGGTGGTTATGAATCGAACACTGTGGTTGCTACCTCTCTTCTAGATATGTACGCGAAATGCGGATCTCTAAGCTGTGCGGAGAAAATCTTCTTGAGAATTCGTTGTCATTCTGTTATTTCGTACACATCTATGATCATGGCGAAGGCAAAGCATGGACTTGGAGAAGCAGCGGTTAAACTATTCGATGAAATGGTTGCAGGGAGAATAAATCCTAACTATGTAACATTACTTGGCGTGTTACACGCTTGTAGCCATTCGGGTCTGGTTAACGAAGGCCTAGAATACTTGAGTTTAATGGCTGAGAAGTATGGTGTGGTTCCTGACTCGAGGCATTACACTTGTGTTGTTGATATGCTTGGAAGATTTGGTCGTGTTGATGAAGCTTATGAATTGGCGAAAACTATAGAAGTTGGAGCAGAGCAAGGTGCGCTCTTGTGGGGAGCTCTTCTCTCAGCTGGTAGGTTACATGGAAGAGTTGAGATTGTAAGTGAAGCGAGCAAGCGATTAATACAATCTAATCAGCAAGTGACAAGTGCGTACATCGCATTATCCAATGCTTATGCTGTGTCTGGAGGATGGGAAGATTCAGAATCTCTTCGATTAGAGATGAAACGTAGTGGAAATGTGAAAGAACGAGCTTGTAGCTGGATTGAAAATAAAGATTCAGTTTATGTTTTTCATGCTGGGGATTTGTCATGTGATGAGAGCGGTGAGATTGAAAGGTTCCTGAAAGATCTAGAGAAGAGAATGAAAGAAAGAGGACACAGAGGAAGTAGTAGTATGATCACAACAAGTTCATCGGTTTTTGTCGATGTTGATGAGGAAGCTAAAGATGAAATGGTGAGTTTGCATTGTGAAAGATTGGCTTTAGCATATGGATTGCTTCATTTGCCTGCAGGATCAACAATTAGGATTATGAACAATTTGAGGATGTGTAGAGATTGCCATGAGGCTTTCAAGCTGATCAGTGAGATTGTGGAGAGGGAAATTGTTGTCAGGGATGTGAATAGGTTTCATTGCTTTAAGAATGGCTCTTGCACTTGTCGTGATTATTGGTGA

Protein Analysis

616

Amino Acids

68.65

Weight (kDa)

8.21

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 93 - 141 3.3e-10 PPR repeat family
PPR_2 PF13041 301 - 348 1.1e-07 PPR repeat family
PPR PF01535 303 - 329 6.6e-06 PPR repeat
E_motif PF20431 420 - 482 6.7e-11 E motif
DYW_deaminase PF14432 530 - 616 4.1e-35 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016096)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15720
fragaria_vesca FvH4_3g12520
malus_domestica MD13G1170500.v1.1
prunus_persica Prupe.1G138900_v2.0.a1
pyrus_communis pycom13g14610
rosa_chinensis RchiOBHm_Chr4g0421681
rosa_laevigata RLG00000007659
rosa_multiflora Rmu_sc0004533.1_g000011
rosa_roxburghii Rroxscaffold_5G00363920
rosa_rugosa Rorug04G0174600
rosa_samantha Rh4AG233900 Rh4BG237000 Rh4CG248800
rosa_wichuraiana Rw0G007720 Rw4G020010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 692
Acc16I TGCGCA 1 cut(s) 630
AccBSI CCGCTC 1 cut(s) 1500
AccII CGCG 2 cut(s) 182, 845
AciI CCGC 5 cut(s) 552, 852, 869, 962, 1500
AclWI GGATC 2 cut(s) 862, 1699
AcoI YGGCCR 1 cut(s) 643
AcsI RAATTY 2 cut(s) 145, 888
AcyI GRCGYC 1 cut(s) 689
AfaI GTAC 5 cut(s) 65, 395, 842, 914, 1331
AfiI CCNNNNNNNGG 1 cut(s) 558
AflIII ACRYGT 2 cut(s) 396, 522
AgsI TTSAA 4 cut(s) 676, 1445, 1511, 1750
AjuI GAANNNNNNNTTGG 4 cut(s) 122, 154, 271, 303
Alw21I GWGCWC 1 cut(s) 1237
AlwI GGATC 2 cut(s) 862, 1699
Ama87I CYCGRG 2 cut(s) 789, 1117
AoxI GGCC 2 cut(s) 643, 1066
ApeKI GCWGC 4 cut(s) 134, 680, 754, 959
ApoI RAATTY 2 cut(s) 145, 888
AseI ATTAAT 1 cut(s) 1298
Asp700I GAANNNNTTC 3 cut(s) 11, 878, 1386
AspLEI GCGC 3 cut(s) 631, 734, 1222
AspS9I GGNCC 1 cut(s) 606
AsuHPI GGTGA 3 cut(s) 197, 1514, 1645
AvaI CYCGRG 2 cut(s) 789, 1117
AvaII GGWCC 1 cut(s) 606
BalI TGGCCA 1 cut(s) 645
BanII GRGCYC 2 cut(s) 486, 1237
BauI CACGAG 1 cut(s) 639
Bbv12I GWGCWC 1 cut(s) 1237
BbvI GCAGC 4 cut(s) 121, 667, 741, 971
BccI CCATC 1 cut(s) 1362
BcgI CGANNNNNNTGC 2 cut(s) 620, 654
BclI TGATCA 4 cut(s) 615, 924, 1575, 1755
BfaI CTAG 8 cut(s) 209, 405, 555, 720, 741, 833, 1070, 1529
BfmI CTRYAG 2 cut(s) 1194, 1686
BglII AGATCT 1 cut(s) 1525
BisI GCNGC 4 cut(s) 135, 681, 755, 960
BlsI GCNGC 4 cut(s) 136, 682, 756, 961
Bme18I GGWCC 1 cut(s) 606
BmeT110I CYCGRG 2 cut(s) 789, 1117
BmgT120I GGNCC 1 cut(s) 606
BmiI GGNNCC 2 cut(s) 1110, 1517
BmsI GCATC 1 cut(s) 351
BplI GAGNNNNNCTC 4 cut(s) 496, 528, 1224, 1256
BpmI CTGGAG 2 cut(s) 491, 1383
BpuEI CTTGAG 2 cut(s) 904, 1099
BsaHI GRCGYC 1 cut(s) 689
Bsc4I CCNNNNNNNGG 1 cut(s) 558
Bse3DI GCAATG 2 cut(s) 764, 1807
BseGI GGATG 5 cut(s) 15, 456, 1373, 1728, 1798
BseLI CCNNNNNNNGG 1 cut(s) 558
BseMI GCAATG 2 cut(s) 764, 1807
BseMII CTCAG 4 cut(s) 74, 499, 1083, 1257
BseXI GCAGC 4 cut(s) 121, 667, 741, 971
BseYI CCCAGC 1 cut(s) 1475
BsgI GTGCAG 1 cut(s) 231
Bsh1236I CGCG 2 cut(s) 182, 845
BshFI GGCC 2 cut(s) 645, 1068
BsiHKAI GWGCWC 1 cut(s) 1237
BsiHKCI CYCGRG 2 cut(s) 789, 1117
BslFI GGGAC 1 cut(s) 41
BslI CCNNNNNNNGG 1 cut(s) 558
BsmFI GGGAC 1 cut(s) 41
BsnI GGCC 2 cut(s) 645, 1068
BsoBI CYCGRG 2 cut(s) 789, 1117
Bsp1286I GDGCHC 2 cut(s) 486, 1237
Bsp143I GATC 8 cut(s) 615, 668, 854, 924, 1525, 1575, 1691, 1755
Bsp68I TCGCGA 1 cut(s) 182
BspACI CCGC 5 cut(s) 552, 852, 869, 962, 1500
BspANI GGCC 2 cut(s) 645, 1068
BspCNI CTCAG 4 cut(s) 73, 498, 1084, 1256
BspFNI CGCG 2 cut(s) 182, 845
BspLI GGNNCC 2 cut(s) 1110, 1517
BspMAI CTGCAG 1 cut(s) 1690
BspPI GGATC 2 cut(s) 862, 1699
BspQI GCTCTTC 1 cut(s) 1242
BsrBI CCGCTC 1 cut(s) 1500
BsrDI GCAATG 2 cut(s) 764, 1807
BssMI GATC 8 cut(s) 615, 668, 854, 924, 1525, 1575, 1691, 1755
BssNI GRCGYC 1 cut(s) 689
BssSI CACGAG 1 cut(s) 639
Bst2BI CACGAG 1 cut(s) 639
Bst4CI ACNGT 3 cut(s) 199, 258, 814
Bst6I CTCTTC 5 cut(s) 834, 1242, 1258, 1392, 1529
BstACI GRCGYC 1 cut(s) 689
BstAPI GCANNNNNTGC 1 cut(s) 425
BstC8I GCNNGC 7 cut(s) 184, 250, 717, 736, 1288, 1292, 1686
BstDEI CTNAG 5 cut(s) 60, 485, 860, 1092, 1243
BstF5I GGATG 5 cut(s) 15, 456, 1373, 1728, 1798
BstFNI CGCG 2 cut(s) 182, 845
BstHHI GCGC 3 cut(s) 631, 734, 1222
BstKTI GATC 8 cut(s) 618, 671, 857, 927, 1528, 1578, 1694, 1758
BstMBI GATC 8 cut(s) 615, 668, 854, 924, 1525, 1575, 1691, 1755
BstMWI GCNNNNNNNGC 5 cut(s) 425, 635, 1219, 1334, 1681
BstNSI RCATGY 3 cut(s) 423, 526, 738
BstSFI CTRYAG 2 cut(s) 1194, 1686
BstUI CGCG 2 cut(s) 182, 845
BstV1I GCAGC 4 cut(s) 121, 667, 741, 971
BstX2I RGATCY 2 cut(s) 854, 1525
BstYI RGATCY 2 cut(s) 854, 1525
BsuRI GGCC 2 cut(s) 645, 1068
BtgZI GCGATG 1 cut(s) 1318
BtsCI GGATG 5 cut(s) 15, 456, 1373, 1728, 1798
BtsI GCAGTG 2 cut(s) 243, 618
BtsIMutI CAGTG 5 cut(s) 195, 243, 618, 810, 1765
BtuMI TCGCGA 1 cut(s) 182
Cac8I GCNNGC 7 cut(s) 184, 250, 717, 736, 1288, 1292, 1686
CfoI GCGC 3 cut(s) 631, 734, 1222
Cfr13I GGNCC 1 cut(s) 606
Csp6I GTAC 5 cut(s) 64, 394, 841, 913, 1330
CviQI GTAC 5 cut(s) 64, 394, 841, 913, 1330
DdeI CTNAG 5 cut(s) 60, 485, 860, 1092, 1243
DpnI GATC 8 cut(s) 617, 670, 856, 926, 1527, 1577, 1693, 1757
DpnII GATC 8 cut(s) 615, 668, 854, 924, 1525, 1575, 1691, 1755
DraI TTTAAA 1 cut(s) 415
EaeI YGGCCR 1 cut(s) 643
Eam1104I CTCTTC 5 cut(s) 834, 1242, 1258, 1392, 1529
EarI CTCTTC 5 cut(s) 834, 1242, 1258, 1392, 1529
Ecl136II GAGCTC 1 cut(s) 1235
Eco147I AGGCCT 1 cut(s) 1068
Eco24I GRGCYC 2 cut(s) 486, 1237
Eco47I GGWCC 1 cut(s) 606
Eco53kI GAGCTC 1 cut(s) 1235
Eco88I CYCGRG 2 cut(s) 789, 1117
EcoICRI GAGCTC 1 cut(s) 1235
EcoRI GAATTC 1 cut(s) 888
EcoT22I ATGCAT 1 cut(s) 366
EcoT38I GRGCYC 2 cut(s) 486, 1237
FalI AAGNNNNNCTT 2 cut(s) 1642, 1674
FaqI GGGAC 1 cut(s) 41
FauNDI CATATG 1 cut(s) 1666
FbaI TGATCA 4 cut(s) 615, 924, 1575, 1755
Fnu4HI GCNGC 4 cut(s) 135, 681, 755, 960
FokI GGATG 5 cut(s) 2, 443, 1380, 1735, 1805
FriOI GRGCYC 2 cut(s) 486, 1237
Fsp4HI GCNGC 4 cut(s) 135, 681, 755, 960
FspBI CTAG 8 cut(s) 209, 405, 555, 720, 741, 833, 1070, 1529
FspI TGCGCA 1 cut(s) 630
GlaI GCGC 3 cut(s) 630, 733, 1221
GluI GCNGC 4 cut(s) 135, 681, 755, 960
GsaI CCCAGC 1 cut(s) 1479
GsuI CTGGAG 2 cut(s) 491, 1383
HaeIII GGCC 2 cut(s) 645, 1068
HhaI GCGC 3 cut(s) 631, 734, 1222
Hin1I GRCGYC 1 cut(s) 689
Hin6I GCGC 3 cut(s) 629, 732, 1220
HinP1I GCGC 3 cut(s) 629, 732, 1220
HincII GTYRAC 2 cut(s) 520, 1060
HindII GTYRAC 2 cut(s) 520, 1060
HindIII AAGCTT 3 cut(s) 96, 113, 1173
HpaI GTTAAC 1 cut(s) 1060
HphI GGTGA 3 cut(s) 197, 1514, 1645
Hpy166II GTNNAC 7 cut(s) 109, 275, 329, 396, 520, 915, 1060
Hpy188I TCNGA 7 cut(s) 121, 162, 168, 357, 488, 694, 1381
Hpy8I GTNNAC 7 cut(s) 109, 275, 329, 396, 520, 915, 1060
Hpy99I CGWCG 1 cut(s) 694
HpyAV CCTTC 3 cut(s) 483, 928, 1058
HpyCH4III ACNGT 3 cut(s) 199, 258, 814
HpyCH4IV ACGT 3 cut(s) 398, 689, 1405
HpyCH4V TGCA 9 cut(s) 41, 248, 364, 537, 757, 989, 1642, 1688, 1830
HpyF10VI GCNNNNNNNGC 5 cut(s) 425, 635, 1219, 1334, 1681
HpyF3I CTNAG 5 cut(s) 60, 485, 860, 1092, 1243
HpySE526I ACGT 3 cut(s) 398, 689, 1405
Hsp92I GRCGYC 1 cut(s) 689
HspAI GCGC 3 cut(s) 629, 732, 1220
Ksp22I TGATCA 4 cut(s) 615, 924, 1575, 1755
KspAI GTTAAC 1 cut(s) 1060
Kzo9I GATC 8 cut(s) 615, 668, 854, 924, 1525, 1575, 1691, 1755
LguI GCTCTTC 1 cut(s) 1242
LmnI GCTCC 2 cut(s) 1205, 1232
Lsp1109I GCAGC 4 cut(s) 121, 667, 741, 971
LweI GCATC 1 cut(s) 351
MaeI CTAG 8 cut(s) 209, 405, 555, 720, 741, 833, 1070, 1529
MaeII ACGT 3 cut(s) 398, 689, 1405
MaeIII GTNAC 5 cut(s) 784, 1012, 1029, 1254, 1318
MalI GATC 8 cut(s) 617, 670, 856, 926, 1527, 1577, 1693, 1757
MbiI CCGCTC 1 cut(s) 1500
MboI GATC 8 cut(s) 615, 668, 854, 924, 1525, 1575, 1691, 1755
MflI RGATCY 2 cut(s) 854, 1525
MhlI GDGCHC 2 cut(s) 486, 1237
MlsI TGGCCA 1 cut(s) 645
MluCI AATT 7 cut(s) 145, 726, 888, 1181, 1698, 1714, 1779
MluNI TGGCCA 1 cut(s) 645
MlyI GAGTC 3 cut(s) 157, 563, 1109
MmeI TCCRAC 2 cut(s) 672, 1183
Mox20I TGGCCA 1 cut(s) 645
Mph1103I ATGCAT 1 cut(s) 366
MroXI GAANNNNTTC 3 cut(s) 11, 878, 1386
MscI TGGCCA 1 cut(s) 645
MseI TTAA 6 cut(s) 414, 966, 1059, 1085, 1298, 1815
MslI CAYNNNNRTG 1 cut(s) 920
Msp20I TGGCCA 1 cut(s) 645
MspA1I CMGCKG 2 cut(s) 962, 1247
MvnI CGCG 2 cut(s) 182, 845
MwoI GCNNNNNNNGC 5 cut(s) 425, 635, 1219, 1334, 1681
NdeI CATATG 1 cut(s) 1666
NdeII GATC 8 cut(s) 615, 668, 854, 924, 1525, 1575, 1691, 1755
NlaIV GGNNCC 2 cut(s) 1110, 1517
NmuCI GTSAC 1 cut(s) 1318
NruI TCGCGA 1 cut(s) 182
NsbI TGCGCA 1 cut(s) 630
NsiI ATGCAT 1 cut(s) 366
NspI RCATGY 3 cut(s) 423, 526, 738
PaeI GCATGC 1 cut(s) 738
PaeR7I CTCGAG 2 cut(s) 789, 1117
PceI AGGCCT 1 cut(s) 1068
PciI ACATGT 1 cut(s) 522
PciSI GCTCTTC 1 cut(s) 1242
PcsI WCGNNNNNNNCGW 1 cut(s) 698
PdmI GAANNNNTTC 3 cut(s) 11, 878, 1386
PfeI GAWTC 9 cut(s) 122, 202, 437, 444, 703, 803, 1376, 1382, 1454
PkrI GCNGC 4 cut(s) 136, 682, 756, 961
PleI GAGTC 3 cut(s) 157, 563, 1109
PpsI GAGTC 3 cut(s) 157, 563, 1109
PscI ACATGT 1 cut(s) 522
PshBI ATTAAT 1 cut(s) 1298
Psp124BI GAGCTC 1 cut(s) 1237
PspFI CCCAGC 1 cut(s) 1475
PspN4I GGNNCC 2 cut(s) 1110, 1517
PspPI GGNCC 1 cut(s) 606
PspXI VCTCGAGB 2 cut(s) 789, 1117
PstI CTGCAG 1 cut(s) 1690
PsuI RGATCY 2 cut(s) 854, 1525
PvuII CAGCTG 1 cut(s) 1247
RruI TCGCGA 1 cut(s) 182
RsaI GTAC 5 cut(s) 65, 395, 842, 914, 1331
RsaNI GTAC 5 cut(s) 64, 394, 841, 913, 1330
RseI CAYNNNNRTG 1 cut(s) 920
SacI GAGCTC 1 cut(s) 1237
SapI GCTCTTC 1 cut(s) 1242
SaqAI TTAA 6 cut(s) 414, 966, 1059, 1085, 1298, 1815
SatI GCNGC 4 cut(s) 135, 681, 755, 960
Sau3AI GATC 8 cut(s) 615, 668, 854, 924, 1525, 1575, 1691, 1755
Sau96I GGNCC 1 cut(s) 606
SbfI CCTGCAGG 1 cut(s) 1690
SchI GAGTC 3 cut(s) 157, 563, 1109
SdaI CCTGCAGG 1 cut(s) 1690
SduI GDGCHC 2 cut(s) 486, 1237
SfaNI GCATC 1 cut(s) 351
SfcI CTRYAG 2 cut(s) 1194, 1686
Sfr274I CTCGAG 2 cut(s) 789, 1117
SinI GGWCC 1 cut(s) 606
SlaI CTCGAG 2 cut(s) 789, 1117
SmiMI CAYNNNNRTG 1 cut(s) 920
SmlI CTYRAG 4 cut(s) 789, 883, 1078, 1117
SmoI CTYRAG 4 cut(s) 789, 883, 1078, 1117
SphI GCATGC 1 cut(s) 738
Sse8387I CCTGCAGG 1 cut(s) 1690
Sse9I AATT 7 cut(s) 145, 726, 888, 1181, 1698, 1714, 1779
SseBI AGGCCT 1 cut(s) 1068
SsiI CCGC 5 cut(s) 552, 852, 869, 962, 1500
SspI AATATT 1 cut(s) 496
SspMI CTAG 8 cut(s) 209, 405, 555, 720, 741, 833, 1070, 1529
SstI GAGCTC 1 cut(s) 1237
StuI AGGCCT 1 cut(s) 1068
TaaI ACNGT 3 cut(s) 199, 258, 814
TaiI ACGT 3 cut(s) 401, 692, 1408
TaqI TCGA 9 cut(s) 567, 572, 611, 790, 806, 975, 1118, 1390, 1602
TasI AATT 7 cut(s) 145, 726, 888, 1181, 1698, 1714, 1779
TatI WGTACW 2 cut(s) 63, 393
TfiI GAWTC 9 cut(s) 122, 202, 437, 444, 703, 803, 1376, 1382, 1454
Tru1I TTAA 6 cut(s) 414, 966, 1059, 1085, 1298, 1815
Tru9I TTAA 6 cut(s) 414, 966, 1059, 1085, 1298, 1815
TscAI CASTG 5 cut(s) 202, 250, 625, 817, 1765
TseFI GTSAC 1 cut(s) 1318
TseI GCWGC 4 cut(s) 134, 680, 754, 959
Tsp45I GTSAC 1 cut(s) 1318
TspRI CASTG 5 cut(s) 202, 250, 625, 817, 1765
VpaK11BI GGWCC 1 cut(s) 606
VspI ATTAAT 1 cut(s) 1298
XapI RAATTY 2 cut(s) 145, 888
XbaI TCTAGA 2 cut(s) 832, 1528
XceI RCATGY 3 cut(s) 423, 526, 738
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XhoI CTCGAG 2 cut(s) 789, 1117
XmnI GAANNNNTTC 3 cut(s) 11, 878, 1386
XspI CTAG 8 cut(s) 209, 405, 555, 720, 741, 833, 1070, 1529
ZraI GACGTC 1 cut(s) 690
Zsp2I ATGCAT 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.