AT4G16160

Mitochondrial import inner membrane translocase subunit Tim17 Tim22 Tim23 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011960
Physical Location & Seq
Forward (+)
9157471 .. 9158933
1463 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G16160.1

Sequence Viewer

Length: 531 bp
ATGGAGAAGAGTGGAGGAAGAATTGTAATGGATGAGATAAGAAGCTTTGAGAAGGCACACTTGTTCGATCTTGGTCATCCTCTTCTTAACCGTATCGCAGATTCCTTCGTTAAAGCCGCCGGAGTGGGAGCTTTACAAGCCGTGTCAAGGGAAGCTTACTTTACCGTGGTTGACGGTTTTGACTCGAACAACGTGGGTCCACCGTCGGAGATTACAGGGAACAAGAAACATAGGTTCCCTAATCTCAGAGGGGAAAGCAGCAAATCTCTTGATGCATTGGTGAAGAACACAGGAAAAGAATCTCTCCAATGGGGGCTAGCGGCAGGGTTATACTCAGGTATCACTTACGGTATGACAGAGGTTCGTGGAGGAGCTCATGATTGGCGGAACAGCGCGGTAGCTGGAGCATTGACAGGAGCGGCAATGGCTATGACGACGTCTGAGAGGACAAGCCATGAGCAAGTGGTTCAGTCTGCTCTCACTGGAGCAGCTATTTCCACCGCTGCTAATCTCCTTTCTAGCGTTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005744 GO:0006605 GO:0006626 GO:0006810 GO:0006811 GO:0006820 GO:0006839 GO:0006865 GO:0006886 GO:0006996 GO:0007005 GO:0008104 GO:0008150 GO:0008509 GO:0008514 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009941 GO:0009987 GO:0015031 GO:0015075 GO:0015171 GO:0015318 GO:0015711 GO:0015833 GO:0015849 GO:0016020 GO:0016021 GO:0016043 GO:0017038 GO:0019866 GO:0019867 GO:0022857 GO:0030150 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031304 GO:0031305 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031966 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0032592 GO:0032991 GO:0033036 GO:0033365 GO:0034220 GO:0034613 GO:0042170 GO:0042802 GO:0042803 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044743 GO:0045184 GO:0046907 GO:0046942 GO:0046943 GO:0046983 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072655 GO:0098573 GO:0098588 GO:0098656 GO:0098796 GO:0098798 GO:0098800 GO:0098805 GO:1903825 GO:1905039 GO:1990542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

176

Amino Acids

18.59

Weight (kDa)

6.98

Isoelectric Point (pI)

29.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 29 - 166 1e-13 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16160 AT4G16160
fragaria_vesca FvH4_4g19460
malus_domestica MD13G1156400.v1.1 MD16G1157300.v1.1
prunus_persica Prupe.1G122200_v2.0.a1 Prupe.1G122200_v2.0.a1
pyrus_communis pycom13g13380
rosa_chinensis RchiOBHm_Chr4g0424511
rosa_laevigata RLG00000007467
rosa_multiflora Rmu_sc0002603.1_g000009 Rmu_sc0003942.1_g000024
rosa_roxburghii Rroxscaffold_5G00366440
rosa_rugosa Rorug04G0198000
rosa_samantha Rh4AG254500 Rh4BG259900 Rh4CG271000 Rh4DG254300
rosa_wichuraiana Rw4G021980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 440
AccBSI CCGCTC 1 cut(s) 419
AccII CGCG 1 cut(s) 395
AciI CCGC 6 cut(s) 117, 320, 385, 395, 419, 501
AcyI GRCGYC 1 cut(s) 437
AfiI CCNNNNNNNGG 1 cut(s) 147
AluBI AGCT 6 cut(s) 45, 131, 155, 374, 401, 491
AluI AGCT 6 cut(s) 45, 131, 155, 374, 401, 491
Alw21I GWGCWC 1 cut(s) 376
ApeKI GCWGC 3 cut(s) 258, 488, 503
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 1 cut(s) 197
AsuHPI GGTGA 1 cut(s) 292
AsuNHI GCTAGC 1 cut(s) 316
AvaII GGWCC 1 cut(s) 197
BaeI ACNNNNGTAYC 2 cut(s) 322, 355
BanII GRGCYC 1 cut(s) 376
Bbv12I GWGCWC 1 cut(s) 376
BbvI GCAGC 3 cut(s) 270, 490, 500
BceAI ACGGC 1 cut(s) 125
BfaI CTAG 3 cut(s) 317, 519, 529
BisI GCNGC 6 cut(s) 117, 259, 321, 420, 489, 504
BlsI GCNGC 6 cut(s) 118, 260, 322, 421, 490, 505
Bme18I GGWCC 1 cut(s) 197
BmgT120I GGNCC 1 cut(s) 197
BmiI GGNNCC 2 cut(s) 198, 236
BmsI GCATC 1 cut(s) 262
BmtI GCTAGC 1 cut(s) 320
BpmI CTGGAG 2 cut(s) 423, 504
BsaHI GRCGYC 1 cut(s) 437
BsaJI CCNNGG 1 cut(s) 165
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse1I ACTGG 1 cut(s) 487
Bse3DI GCAATG 1 cut(s) 429
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 2 cut(s) 37, 76
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 3 cut(s) 259, 348, 432
BseNI ACTGG 1 cut(s) 487
BseRI GAGGAG 1 cut(s) 384
BseXI GCAGC 3 cut(s) 270, 490, 500
Bsh1236I CGCG 1 cut(s) 395
BsiHKAI GWGCWC 1 cut(s) 376
BsiSI CCGG 1 cut(s) 120
BslI CCNNNNNNNGG 1 cut(s) 147
Bsp1286I GDGCHC 1 cut(s) 376
Bsp143I GATC 1 cut(s) 67
BspACI CCGC 6 cut(s) 117, 320, 385, 395, 419, 501
BspCNI CTCAG 3 cut(s) 258, 347, 433
BspFNI CGCG 1 cut(s) 395
BspHI TCATGA 1 cut(s) 376
BspLI GGNNCC 2 cut(s) 198, 236
BspOI GCTAGC 1 cut(s) 320
BsrBI CCGCTC 1 cut(s) 419
BsrDI GCAATG 1 cut(s) 429
BsrI ACTGG 1 cut(s) 487
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 1 cut(s) 67
BssNI GRCGYC 1 cut(s) 437
Bst4CI ACNGT 5 cut(s) 92, 166, 176, 204, 350
Bst6I CTCTTC 2 cut(s) 2, 87
BstACI GRCGYC 1 cut(s) 437
BstC8I GCNNGC 1 cut(s) 318
BstDEI CTNAG 3 cut(s) 245, 334, 441
BstDSI CCRYGG 1 cut(s) 165
BstF5I GGATG 2 cut(s) 37, 76
BstFNI CGCG 1 cut(s) 395
BstHHI GCGC 1 cut(s) 395
BstKTI GATC 1 cut(s) 70
BstMBI GATC 1 cut(s) 67
BstMWI GCNNNNNNNGC 2 cut(s) 137, 425
BstUI CGCG 1 cut(s) 395
BstV1I GCAGC 3 cut(s) 270, 490, 500
BtgI CCRYGG 1 cut(s) 165
BtsCI GGATG 2 cut(s) 37, 76
BtsIMutI CAGTG 1 cut(s) 480
Cac8I GCNNGC 1 cut(s) 318
CciI TCATGA 1 cut(s) 376
CfoI GCGC 1 cut(s) 395
Cfr13I GGNCC 1 cut(s) 197
CviAII CATG 2 cut(s) 377, 455
DdeI CTNAG 3 cut(s) 245, 334, 441
DpnI GATC 1 cut(s) 69
DpnII GATC 1 cut(s) 67
Eam1104I CTCTTC 2 cut(s) 2, 87
EarI CTCTTC 2 cut(s) 2, 87
EciI GGCGGA 1 cut(s) 400
Ecl136II GAGCTC 1 cut(s) 374
Eco24I GRGCYC 1 cut(s) 376
Eco47I GGWCC 1 cut(s) 197
Eco53kI GAGCTC 1 cut(s) 374
EcoICRI GAGCTC 1 cut(s) 374
EcoT22I ATGCAT 1 cut(s) 277
EcoT38I GRGCYC 1 cut(s) 376
FaeI CATG 2 cut(s) 380, 458
FaiI YATR 6 cut(s) 231, 331, 353, 378, 431, 456
FalI AAGNNNNNCTT 4 cut(s) 44, 76, 139, 171
FatI CATG 2 cut(s) 376, 454
Fnu4HI GCNGC 6 cut(s) 117, 259, 321, 420, 489, 504
FokI GGATG 2 cut(s) 44, 63
FriOI GRGCYC 1 cut(s) 376
Fsp4HI GCNGC 6 cut(s) 117, 259, 321, 420, 489, 504
FspBI CTAG 3 cut(s) 317, 519, 529
GlaI GCGC 1 cut(s) 394
GluI GCNGC 6 cut(s) 117, 259, 321, 420, 489, 504
GsuI CTGGAG 2 cut(s) 423, 504
HapII CCGG 1 cut(s) 120
HhaI GCGC 1 cut(s) 395
Hin1I GRCGYC 1 cut(s) 437
Hin1II CATG 2 cut(s) 380, 458
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 2 cut(s) 43, 153
HinfI GANTC 3 cut(s) 101, 182, 299
HpaII CCGG 1 cut(s) 120
HphI GGTGA 1 cut(s) 292
Hpy166II GTNNAC 2 cut(s) 172, 200
Hpy188I TCNGA 3 cut(s) 208, 248, 442
Hpy188III TCNNGA 2 cut(s) 269, 377
Hpy8I GTNNAC 2 cut(s) 172, 200
Hpy99I CGWCG 2 cut(s) 208, 439
HpyAV CCTTC 2 cut(s) 46, 115
HpyCH4III ACNGT 5 cut(s) 92, 166, 176, 204, 350
HpyCH4IV ACGT 2 cut(s) 192, 437
HpyCH4V TGCA 1 cut(s) 275
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 425
HpyF3I CTNAG 3 cut(s) 245, 334, 441
HpySE526I ACGT 2 cut(s) 192, 437
Hsp92I GRCGYC 1 cut(s) 437
Hsp92II CATG 2 cut(s) 380, 458
HspAI GCGC 1 cut(s) 393
Kzo9I GATC 1 cut(s) 67
LmnI GCTCC 5 cut(s) 128, 371, 404, 416, 485
LpnPI CCDG 8 cut(s) 133, 201, 276, 309, 321, 387, 399, 468
Lsp1109I GCAGC 3 cut(s) 270, 490, 500
LweI GCATC 1 cut(s) 262
MaeI CTAG 3 cut(s) 317, 519, 529
MaeII ACGT 2 cut(s) 192, 437
MalI GATC 1 cut(s) 69
MbiI CCGCTC 1 cut(s) 419
MboI GATC 1 cut(s) 67
MboII GAAGA 4 cut(s) 19, 30, 74, 295
MhlI GDGCHC 1 cut(s) 376
MluCI AATT 1 cut(s) 21
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 186
MnlI CCTC 6 cut(s) 8, 90, 242, 352, 362, 438
Mph1103I ATGCAT 1 cut(s) 277
MseI TTAA 2 cut(s) 87, 111
MspA1I CMGCKG 1 cut(s) 503
MspI CCGG 1 cut(s) 120
MvnI CGCG 1 cut(s) 395
MwoI GCNNNNNNNGC 2 cut(s) 137, 425
NdeII GATC 1 cut(s) 67
NheI GCTAGC 1 cut(s) 316
NlaIII CATG 2 cut(s) 380, 458
NlaIV GGNNCC 2 cut(s) 198, 236
NsiI ATGCAT 1 cut(s) 277
PagI TCATGA 1 cut(s) 376
PfeI GAWTC 2 cut(s) 101, 299
PflFI GACNNNGTC 1 cut(s) 436
PkrI GCNGC 6 cut(s) 118, 260, 322, 421, 490, 505
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
Psp124BI GAGCTC 1 cut(s) 376
PspN4I GGNNCC 2 cut(s) 198, 236
PspPI GGNCC 1 cut(s) 197
PsyI GACNNNGTC 1 cut(s) 436
SacI GAGCTC 1 cut(s) 376
SaqAI TTAA 2 cut(s) 87, 111
SatI GCNGC 6 cut(s) 117, 259, 321, 420, 489, 504
Sau3AI GATC 1 cut(s) 67
Sau96I GGNCC 1 cut(s) 197
SchI GAGTC 1 cut(s) 176
SduI GDGCHC 1 cut(s) 376
SfaNI GCATC 1 cut(s) 262
SinI GGWCC 1 cut(s) 197
Sse9I AATT 1 cut(s) 21
SsiI CCGC 6 cut(s) 117, 320, 385, 395, 419, 501
SspMI CTAG 3 cut(s) 317, 519, 529
SstI GAGCTC 1 cut(s) 376
TaaI ACNGT 5 cut(s) 92, 166, 176, 204, 350
TaiI ACGT 2 cut(s) 195, 440
TaqI TCGA 2 cut(s) 66, 185
TasI AATT 1 cut(s) 21
TauI GCSGC 3 cut(s) 119, 323, 422
TfiI GAWTC 2 cut(s) 101, 299
Tru1I TTAA 2 cut(s) 87, 111
Tru9I TTAA 2 cut(s) 87, 111
TscAI CASTG 1 cut(s) 487
TseI GCWGC 3 cut(s) 258, 488, 503
TspRI CASTG 1 cut(s) 487
Tth111I GACNNNGTC 1 cut(s) 436
VpaK11BI GGWCC 1 cut(s) 197
XspI CTAG 3 cut(s) 317, 519, 529
ZraI GACGTC 1 cut(s) 438
Zsp2I ATGCAT 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.