MD13G1156400.v1.1

Outer envelope pore protein 16-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011960
Physical Location & Seq
Reverse (-)
12183796 .. 12185982
2187 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1156400.v1.1.491

Sequence Viewer

Length: 690 bp
ATGAGTACGTGTCTTCCTTGTACCTTCTTGCCTCATTTTCTACTGGTTCCCCGCTTCAGTCTCATCACTCATCACAGCAGTATAACCTCAAACTTTTACGATATCGAACAAGTAATACTGAAGCAGTGTGAAGATATGTTGAACACGACCAGCAGCACTAGTTTGGAAACAAAGCCGTCGTTGCTGCCGGAGCTGCGCAGCTTCGACAAGAGCGGCTTCTTCGACCTCGGCCACCCTCTCCTCAACCGCATCGCCGAGAGCTTCGTCAAGGCCGCTGGGATCGGAGCTATTCAGGCTGTGTCGCGTGAGGCTTATTTCATAGCCATTGAAGGATTCGATTCAAGTACAAATGGCATTCCGCCCGAAATCTCAGTTCCAGGAAACAAAAGGCAACGCTTCCCTGACCTCAGAGGTGAAAACAACAGAAAATCTCTTGAAGCTATGGTGAAGAATACAGGAAAGGAATCATTACAATGGGGGCTGGCTGCGGGGGTGTATTCGGGTCTTACATATGGCCTGGCAGAGGCTCGTGGAGCTCACGATTGGAAAAACAGTGCAGTTGCGGGAGCGATTACAGGCGTTGCCCTGGCACTCACATCGGAGGGTTCGTCCCATGAGCAGATAGTGCAAGGCGCCATCACCGGAGCTGCCATATCCACCGCTGCAAATCTTCTTTCTGGGATATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

230

Amino Acids

24.5

Weight (kDa)

6.06

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 81 - 219 6e-16 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16160 AT4G16160
fragaria_vesca FvH4_4g19460
malus_domestica MD13G1156400.v1.1 MD16G1157300.v1.1
prunus_persica Prupe.1G122200_v2.0.a1 Prupe.1G122200_v2.0.a1
pyrus_communis pycom13g13380
rosa_chinensis RchiOBHm_Chr4g0424511
rosa_laevigata RLG00000007467
rosa_multiflora Rmu_sc0002603.1_g000009 Rmu_sc0003942.1_g000024
rosa_roxburghii Rroxscaffold_5G00366440
rosa_rugosa Rorug04G0198000
rosa_samantha Rh4AG254500 Rh4BG259900 Rh4CG271000 Rh4DG254300
rosa_wichuraiana Rw4G021980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 197
AccB1I GGYRCC 1 cut(s) 632
AccBSI CCGCTC 1 cut(s) 213
AccII CGCG 1 cut(s) 304
AciI CCGC 8 cut(s) 52, 213, 247, 273, 359, 488, 563, 660
AclWI GGATC 1 cut(s) 287
AcoI YGGCCR 1 cut(s) 229
AcuI CTGAAG 2 cut(s) 40, 140
AcyI GRCGYC 1 cut(s) 633
AfaI GTAC 3 cut(s) 7, 22, 346
AfiI CCNNNNNNNGG 1 cut(s) 523
AflIII ACRYGT 1 cut(s) 8
AgsI TTSAA 4 cut(s) 142, 329, 342, 437
AhlI ACTAGT 1 cut(s) 158
AjnI CCWGG 3 cut(s) 376, 516, 585
AluBI AGCT 7 cut(s) 193, 201, 261, 287, 440, 536, 647
AluI AGCT 7 cut(s) 193, 201, 261, 287, 440, 536, 647
Alw21I GWGCWC 1 cut(s) 538
Alw26I GTCTC 1 cut(s) 65
AlwI GGATC 1 cut(s) 287
AoxI GGCC 3 cut(s) 229, 270, 514
ApeKI GCWGC 7 cut(s) 153, 184, 193, 198, 485, 647, 662
AspLEI GCGC 2 cut(s) 198, 635
AsuHPI GGTGA 3 cut(s) 425, 457, 631
BanI GGYRCC 1 cut(s) 632
BanII GRGCYC 1 cut(s) 538
BauI CACGAG 1 cut(s) 528
BbsI GAAGAC 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 538
BbvI GCAGC 7 cut(s) 165, 171, 180, 210, 472, 634, 649
BccI CCATC 1 cut(s) 644
BceAI ACGGC 1 cut(s) 160
BcgI CGANNNNNNTGC 2 cut(s) 579, 613
BciT130I CCWGG 3 cut(s) 378, 518, 587
BcoDI GTCTC 1 cut(s) 65
BcuI ACTAGT 1 cut(s) 158
BfaI CTAG 1 cut(s) 159
BfoI RGCGCY 1 cut(s) 636
BisI GCNGC 9 cut(s) 154, 185, 194, 199, 214, 273, 486, 648, 663
BlsI GCNGC 9 cut(s) 155, 186, 195, 200, 215, 274, 487, 649, 664
Bme1390I CCNGG 3 cut(s) 378, 518, 587
BmiI GGNNCC 2 cut(s) 48, 634
BmrFI CCNGG 3 cut(s) 378, 518, 587
BmsI GCATC 1 cut(s) 258
BpiI GAAGAC 1 cut(s) 5
BsaAI YACGTR 1 cut(s) 9
BsaHI GRCGYC 1 cut(s) 633
BsaJI CCNNGG 2 cut(s) 226, 585
BsaWI WCCGGW 1 cut(s) 641
BsaXI ACNNNNNCTCC 2 cut(s) 593, 623
Bsc4I CCNNNNNNNGG 1 cut(s) 523
Bse1I ACTGG 1 cut(s) 48
BseBI CCWGG 3 cut(s) 378, 518, 587
BseDI CCNNGG 2 cut(s) 226, 585
BseLI CCNNNNNNNGG 1 cut(s) 523
BseMII CTCAG 2 cut(s) 384, 421
BseNI ACTGG 1 cut(s) 48
BseRI GAGGAG 1 cut(s) 230
BseXI GCAGC 7 cut(s) 165, 171, 180, 210, 472, 634, 649
BseYI CCCAGC 1 cut(s) 275
BsgI GTGCAG 1 cut(s) 576
Bsh1236I CGCG 1 cut(s) 304
BshFI GGCC 3 cut(s) 231, 272, 516
BshNI GGYRCC 1 cut(s) 632
BsiHKAI GWGCWC 1 cut(s) 538
BsiSI CCGG 2 cut(s) 188, 642
BslFI GGGAC 1 cut(s) 595
BslI CCNNNNNNNGG 1 cut(s) 523
BsmAI GTCTC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 595
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 3 cut(s) 231, 272, 516
Bsp1286I GDGCHC 1 cut(s) 538
Bsp143I GATC 1 cut(s) 279
BspACI CCGC 8 cut(s) 52, 213, 247, 273, 359, 488, 563, 660
BspANI GGCC 3 cut(s) 231, 272, 516
BspCNI CTCAG 2 cut(s) 383, 420
BspFNI CGCG 1 cut(s) 304
BspLI GGNNCC 2 cut(s) 48, 634
BspPI GGATC 1 cut(s) 287
BspT107I GGYRCC 1 cut(s) 632
BsrBI CCGCTC 1 cut(s) 213
BsrI ACTGG 1 cut(s) 48
BssECI CCNNGG 2 cut(s) 226, 585
BssMI GATC 1 cut(s) 279
BssNI GRCGYC 1 cut(s) 633
BssSI CACGAG 1 cut(s) 528
Bst2BI CACGAG 1 cut(s) 528
Bst2UI CCWGG 3 cut(s) 378, 518, 587
Bst4CI ACNGT 1 cut(s) 554
BstACI GRCGYC 1 cut(s) 633
BstAPI GCANNNNNTGC 1 cut(s) 625
BstBAI YACGTR 1 cut(s) 9
BstC8I GCNNGC 1 cut(s) 483
BstDEI CTNAG 2 cut(s) 370, 407
BstENI CCTNNNNNAGG 1 cut(s) 521
BstFNI CGCG 1 cut(s) 304
BstH2I RGCGCY 1 cut(s) 636
BstHHI GCGC 2 cut(s) 198, 635
BstKTI GATC 1 cut(s) 282
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 1 cut(s) 279
BstMWI GCNNNNNNNGC 6 cut(s) 181, 190, 193, 293, 533, 625
BstNI CCWGG 3 cut(s) 378, 518, 587
BstSCI CCNGG 3 cut(s) 376, 516, 585
BstUI CGCG 1 cut(s) 304
BstV1I GCAGC 7 cut(s) 165, 171, 180, 210, 472, 634, 649
BstV2I GAAGAC 1 cut(s) 5
BsuRI GGCC 3 cut(s) 231, 272, 516
BtgZI GCGATG 1 cut(s) 235
BtsI GCAGTG 1 cut(s) 131
BtsIMutI CAGTG 2 cut(s) 131, 559
Cac8I GCNNGC 1 cut(s) 483
CfoI GCGC 2 cut(s) 198, 635
Csp6I GTAC 3 cut(s) 6, 21, 345
CviAII CATG 1 cut(s) 614
CviQI GTAC 3 cut(s) 6, 21, 345
DdeI CTNAG 2 cut(s) 370, 407
DinI GGCGCC 1 cut(s) 634
DpnI GATC 1 cut(s) 281
DpnII GATC 1 cut(s) 279
EaeI YGGCCR 1 cut(s) 229
EciI GGCGGA 1 cut(s) 348
Ecl136II GAGCTC 1 cut(s) 536
Eco24I GRGCYC 1 cut(s) 538
Eco32I GATATC 1 cut(s) 103
Eco53kI GAGCTC 1 cut(s) 536
Eco57I CTGAAG 2 cut(s) 40, 140
EcoICRI GAGCTC 1 cut(s) 536
EcoNI CCTNNNNNAGG 1 cut(s) 521
EcoRII CCWGG 3 cut(s) 376, 516, 585
EcoRV GATATC 1 cut(s) 103
EcoT38I GRGCYC 1 cut(s) 538
EgeI GGCGCC 1 cut(s) 634
EheI GGCGCC 1 cut(s) 634
FaeI CATG 1 cut(s) 617
FaiI YATR 8 cut(s) 83, 137, 320, 443, 511, 513, 615, 653
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 1 cut(s) 595
FatI CATG 1 cut(s) 613
FauI CCCGC 3 cut(s) 59, 481, 556
FauNDI CATATG 1 cut(s) 511
Fnu4HI GCNGC 9 cut(s) 154, 185, 194, 199, 214, 273, 486, 648, 663
FriOI GRGCYC 1 cut(s) 538
Fsp4HI GCNGC 9 cut(s) 154, 185, 194, 199, 214, 273, 486, 648, 663
FspBI CTAG 1 cut(s) 159
FspI TGCGCA 1 cut(s) 197
GlaI GCGC 2 cut(s) 197, 634
GluI GCNGC 9 cut(s) 154, 185, 194, 199, 214, 273, 486, 648, 663
GsaI CCCAGC 1 cut(s) 279
HaeII RGCGCY 1 cut(s) 636
HaeIII GGCC 3 cut(s) 231, 272, 516
HapII CCGG 2 cut(s) 188, 642
HhaI GCGC 2 cut(s) 198, 635
Hin1I GRCGYC 1 cut(s) 633
Hin1II CATG 1 cut(s) 617
Hin6I GCGC 2 cut(s) 196, 633
HinP1I GCGC 2 cut(s) 196, 633
HinfI GANTC 3 cut(s) 333, 338, 464
HpaII CCGG 2 cut(s) 188, 642
HphI GGTGA 3 cut(s) 425, 457, 631
Hpy188I TCNGA 3 cut(s) 284, 410, 601
Hpy188III TCNNGA 2 cut(s) 434, 539
Hpy99I CGWCG 1 cut(s) 181
HpyAV CCTTC 2 cut(s) 34, 323
HpyCH4III ACNGT 1 cut(s) 554
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 3 cut(s) 557, 628, 665
HpyF10VI GCNNNNNNNGC 6 cut(s) 181, 190, 193, 293, 533, 625
HpyF3I CTNAG 2 cut(s) 370, 407
HpySE526I ACGT 1 cut(s) 8
Hsp92I GRCGYC 1 cut(s) 633
Hsp92II CATG 1 cut(s) 617
HspAI GCGC 2 cut(s) 196, 633
KasI GGCGCC 1 cut(s) 632
Kzo9I GATC 1 cut(s) 279
LmnI GCTCC 5 cut(s) 190, 284, 533, 566, 644
Lsp1109I GCAGC 7 cut(s) 165, 171, 180, 210, 472, 634, 649
LweI GCATC 1 cut(s) 258
MaeI CTAG 1 cut(s) 159
MaeII ACGT 1 cut(s) 8
MalI GATC 1 cut(s) 281
MbiI CCGCTC 1 cut(s) 213
MboI GATC 1 cut(s) 279
MboII GAAGA 5 cut(s) 5, 143, 211, 460, 662
MhlI GDGCHC 1 cut(s) 538
Mly113I GGCGCC 1 cut(s) 633
MseI TTAA 1 cut(s) 688
MslI CAYNNNNRTG 1 cut(s) 472
MspA1I CMGCKG 2 cut(s) 275, 662
MspI CCGG 2 cut(s) 188, 642
MspR9I CCNGG 3 cut(s) 378, 518, 587
Mva1269I GAATGC 1 cut(s) 354
MvaI CCWGG 3 cut(s) 378, 518, 587
MvnI CGCG 1 cut(s) 304
MwoI GCNNNNNNNGC 6 cut(s) 181, 190, 193, 293, 533, 625
NarI GGCGCC 1 cut(s) 633
NdeI CATATG 1 cut(s) 511
NdeII GATC 1 cut(s) 279
NlaIII CATG 1 cut(s) 617
NlaIV GGNNCC 2 cut(s) 48, 634
NmeAIII GCCGAG 2 cut(s) 207, 280
NsbI TGCGCA 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 605
PctI GAATGC 1 cut(s) 354
PfeI GAWTC 3 cut(s) 333, 338, 464
PfoI TCCNGGA 1 cut(s) 376
PkrI GCNGC 9 cut(s) 155, 186, 195, 200, 215, 274, 487, 649, 664
PluTI GGCGCC 1 cut(s) 636
Ppu21I YACGTR 1 cut(s) 9
Psp124BI GAGCTC 1 cut(s) 538
Psp6I CCWGG 3 cut(s) 376, 516, 585
PspFI CCCAGC 1 cut(s) 275
PspGI CCWGG 3 cut(s) 376, 516, 585
PspN4I GGNNCC 2 cut(s) 48, 634
PsrI GAACNNNNNNTAC 2 cut(s) 99, 131
RsaI GTAC 3 cut(s) 7, 22, 346
RsaNI GTAC 3 cut(s) 6, 21, 345
RseI CAYNNNNRTG 1 cut(s) 472
SacI GAGCTC 1 cut(s) 538
SaqAI TTAA 1 cut(s) 688
SatI GCNGC 9 cut(s) 154, 185, 194, 199, 214, 273, 486, 648, 663
Sau3AI GATC 1 cut(s) 279
ScrFI CCNGG 3 cut(s) 378, 518, 587
SduI GDGCHC 1 cut(s) 538
SfaNI GCATC 1 cut(s) 258
SfoI GGCGCC 1 cut(s) 634
SmiMI CAYNNNNRTG 1 cut(s) 472
SpeI ACTAGT 1 cut(s) 158
SsiI CCGC 8 cut(s) 52, 213, 247, 273, 359, 488, 563, 660
SspDI GGCGCC 1 cut(s) 632
SspMI CTAG 1 cut(s) 159
SstI GAGCTC 1 cut(s) 538
StyD4I CCNGG 3 cut(s) 376, 516, 585
TaaI ACNGT 1 cut(s) 554
TaiI ACGT 1 cut(s) 11
TaqI TCGA 4 cut(s) 105, 204, 222, 336
TatI WGTACW 1 cut(s) 344
TauI GCSGC 2 cut(s) 216, 275
TfiI GAWTC 3 cut(s) 333, 338, 464
Tru1I TTAA 1 cut(s) 688
Tru9I TTAA 1 cut(s) 688
TscAI CASTG 2 cut(s) 131, 559
TseI GCWGC 7 cut(s) 153, 184, 193, 198, 485, 647, 662
TspDTI ATGAA 1 cut(s) 307
TspRI CASTG 2 cut(s) 131, 559
XagI CCTNNNNNAGG 1 cut(s) 521
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.