FvH4_4g19460

Outer envelope pore protein 16-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011960
Physical Location & Seq
Forward (+)
23098126 .. 23100177
2052 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19460.t1

Sequence Viewer

Length: 558 bp
ATGTTGAACAGCGGGGGAAGTGTTAACACTCATATCGAGGCTTCGAAGCCGTCGTCGTTTGAGTTCCCTGGATTTGAGAAGGGTGCCATGTTCGACCTCGGCCACCCTCTCCTCAACCGCATCGCCGAGAGCTTTGTCAAGGCCGCCGGGATTGGAGCTGTTCAAGCTGTGTCACGAGAGGCTTATTTCACTGCCACTGAAGCTTCAGGACTTGGTTCAAGTAATGGGACGCCGCCGGATATCTCAGGCCACAAAAAGAATCGCTTCCCGGACCTCAGAGGGGGTAACAGCAGTAAATCTCTTGAAGCTATGGTGAAGCACACAGGAAAGGAATCCTTACAATGGGGGCTAGCTGCAGGGGTGTACTCGGGGCTTACTTATGGACTGAAGGAGGCTCGTGGAGCCCACGACTGGAAAAACAGTGCAGTTGCAGGAGCCATTACTGGTGTGGCTCTGGCACTTACATCCGACTGTTCCTCTCATGAGCAGATTGTGCAATGTGCTATCACTGGAGCTGCCATTTCCAGTGCTGCAAATCTTCTTTCTGGGATATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

186

Amino Acids

19.03

Weight (kDa)

7.01

Isoelectric Point (pI)

21.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 38 - 175 4.4e-16 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16160 AT4G16160
fragaria_vesca FvH4_4g19460
malus_domestica MD13G1156400.v1.1 MD16G1157300.v1.1
prunus_persica Prupe.1G122200_v2.0.a1 Prupe.1G122200_v2.0.a1
pyrus_communis pycom13g13380
rosa_chinensis RchiOBHm_Chr4g0424511
rosa_laevigata RLG00000007467
rosa_multiflora Rmu_sc0002603.1_g000009 Rmu_sc0003942.1_g000024
rosa_roxburghii Rroxscaffold_5G00366440
rosa_rugosa Rorug04G0198000
rosa_samantha Rh4AG254500 Rh4BG259900 Rh4CG271000 Rh4DG254300
rosa_wichuraiana Rw4G021980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 83
AciI CCGC 4 cut(s) 12, 118, 144, 233
AcoI YGGCCR 1 cut(s) 100
AcuI CTGAAG 3 cut(s) 189, 219, 407
AcyI GRCGYC 1 cut(s) 230
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 3 cut(s) 280, 342, 411
AgsI TTSAA 4 cut(s) 7, 164, 219, 305
AjnI CCWGG 1 cut(s) 67
AluBI AGCT 7 cut(s) 132, 158, 167, 203, 308, 353, 515
AluI AGCT 7 cut(s) 132, 158, 167, 203, 308, 353, 515
Ama87I CYCGRG 1 cut(s) 367
AoxI GGCC 3 cut(s) 100, 141, 247
ApeKI GCWGC 3 cut(s) 353, 515, 530
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
Asp700I GAANNNNTTC 1 cut(s) 263
AspS9I GGNCC 1 cut(s) 271
AsuC2I CCSGG 2 cut(s) 148, 269
AsuHPI GGTGA 1 cut(s) 325
AsuII TTCGAA 1 cut(s) 44
AsuNHI GCTAGC 1 cut(s) 349
AvaI CYCGRG 1 cut(s) 367
AvaII GGWCC 1 cut(s) 271
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 1 cut(s) 406
BauI CACGAG 2 cut(s) 174, 396
BbvI GCAGC 3 cut(s) 340, 502, 517
BceAI ACGGC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 69
BcnI CCSGG 2 cut(s) 148, 269
BfaI CTAG 1 cut(s) 350
BfmI CTRYAG 1 cut(s) 354
BisI GCNGC 5 cut(s) 144, 233, 354, 516, 531
BlsI GCNGC 5 cut(s) 145, 234, 355, 517, 532
Bme1390I CCNGG 3 cut(s) 69, 148, 269
Bme18I GGWCC 1 cut(s) 271
BmeT110I CYCGRG 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 271
BmiI GGNNCC 3 cut(s) 85, 403, 436
BmrFI CCNGG 3 cut(s) 69, 148, 269
BmsI GCATC 1 cut(s) 129
BmtI GCTAGC 1 cut(s) 353
BpmI CTGGAG 1 cut(s) 531
Bpu14I TTCGAA 1 cut(s) 44
BpuMI CCSGG 2 cut(s) 148, 269
BsaHI GRCGYC 1 cut(s) 230
BsaJI CCNNGG 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 3 cut(s) 280, 342, 411
Bse1I ACTGG 4 cut(s) 416, 448, 514, 525
Bse3DI GCAATG 1 cut(s) 503
BseBI CCWGG 1 cut(s) 69
BseDI CCNNGG 2 cut(s) 67, 97
BseGI GGATG 1 cut(s) 464
BseLI CCNNNNNNNGG 3 cut(s) 280, 342, 411
BseMI GCAATG 1 cut(s) 503
BseMII CTCAG 2 cut(s) 258, 289
BseNI ACTGG 4 cut(s) 416, 448, 514, 525
BseRI GAGGAG 1 cut(s) 101
BseXI GCAGC 3 cut(s) 340, 502, 517
BsgI GTGCAG 1 cut(s) 444
BshFI GGCC 3 cut(s) 102, 143, 249
BshNI GGYRCC 1 cut(s) 83
BsiHKCI CYCGRG 1 cut(s) 367
BsiSI CCGG 3 cut(s) 147, 236, 269
BslFI GGGAC 1 cut(s) 241
BslI CCNNNNNNNGG 3 cut(s) 280, 342, 411
BsmFI GGGAC 1 cut(s) 241
BsnI GGCC 3 cut(s) 102, 143, 249
BsoBI CYCGRG 1 cut(s) 367
Bsp119I TTCGAA 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 406
BspACI CCGC 4 cut(s) 12, 118, 144, 233
BspANI GGCC 3 cut(s) 102, 143, 249
BspCNI CTCAG 2 cut(s) 257, 288
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 3 cut(s) 85, 403, 436
BspMAI CTGCAG 1 cut(s) 358
BspOI GCTAGC 1 cut(s) 353
BspT104I TTCGAA 1 cut(s) 44
BspT107I GGYRCC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 503
BsrI ACTGG 4 cut(s) 416, 448, 514, 525
BssECI CCNNGG 2 cut(s) 67, 97
BssNI GRCGYC 1 cut(s) 230
BssSI CACGAG 2 cut(s) 174, 396
Bst2BI CACGAG 2 cut(s) 174, 396
Bst2UI CCWGG 1 cut(s) 69
Bst4CI ACNGT 2 cut(s) 422, 473
BstACI GRCGYC 1 cut(s) 230
BstAPI GCANNNNNTGC 1 cut(s) 493
BstBI TTCGAA 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 351
BstDEI CTNAG 2 cut(s) 244, 275
BstF5I GGATG 1 cut(s) 464
BstMWI GCNNNNNNNGC 4 cut(s) 164, 200, 401, 493
BstNI CCWGG 1 cut(s) 69
BstSCI CCNGG 3 cut(s) 67, 146, 267
BstSFI CTRYAG 1 cut(s) 354
BstV1I GCAGC 3 cut(s) 340, 502, 517
BsuRI GGCC 3 cut(s) 102, 143, 249
BtgZI GCGATG 1 cut(s) 106
BtsCI GGATG 1 cut(s) 464
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 5 cut(s) 189, 195, 427, 507, 532
Cac8I GCNNGC 1 cut(s) 351
CciI TCATGA 1 cut(s) 481
Cfr13I GGNCC 1 cut(s) 271
CseI GACGC 1 cut(s) 238
Csp6I GTAC 1 cut(s) 364
CviAII CATG 2 cut(s) 88, 482
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 2 cut(s) 244, 275
EaeI YGGCCR 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 406
Eco32I GATATC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 271
Eco57I CTGAAG 3 cut(s) 189, 219, 407
Eco88I CYCGRG 1 cut(s) 367
EcoRII CCWGG 1 cut(s) 67
EcoRV GATATC 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 406
FaeI CATG 2 cut(s) 91, 485
FaiI YATR 5 cut(s) 33, 89, 311, 381, 483
FalI AAGNNNNNCTT 4 cut(s) 248, 280, 320, 352
FaqI GGGAC 1 cut(s) 241
FatI CATG 2 cut(s) 87, 481
FauI CCCGC 1 cut(s) 5
Fnu4HI GCNGC 5 cut(s) 144, 233, 354, 516, 531
FokI GGATG 1 cut(s) 451
FriOI GRGCYC 1 cut(s) 406
Fsp4HI GCNGC 5 cut(s) 144, 233, 354, 516, 531
FspBI CTAG 1 cut(s) 350
GluI GCNGC 5 cut(s) 144, 233, 354, 516, 531
GsuI CTGGAG 1 cut(s) 531
HaeIII GGCC 3 cut(s) 102, 143, 249
HapII CCGG 3 cut(s) 147, 236, 269
HgaI GACGC 1 cut(s) 238
Hin1I GRCGYC 1 cut(s) 230
Hin1II CATG 2 cut(s) 91, 485
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HindIII AAGCTT 1 cut(s) 201
HinfI GANTC 2 cut(s) 259, 332
HpaI GTTAAC 1 cut(s) 25
HpaII CCGG 3 cut(s) 147, 236, 269
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 2 cut(s) 25, 364
Hpy188I TCNGA 2 cut(s) 278, 469
Hpy188III TCNNGA 4 cut(s) 174, 207, 302, 482
Hpy8I GTNNAC 2 cut(s) 25, 364
Hpy99I CGWCG 2 cut(s) 55, 58
HpyAV CCTTC 2 cut(s) 73, 382
HpyCH4III ACNGT 2 cut(s) 422, 473
HpyCH4V TGCA 5 cut(s) 356, 425, 431, 496, 533
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 200, 401, 493
HpyF3I CTNAG 2 cut(s) 244, 275
Hsp92I GRCGYC 1 cut(s) 230
Hsp92II CATG 2 cut(s) 91, 485
KspAI GTTAAC 1 cut(s) 25
LmnI GCTCC 4 cut(s) 155, 401, 434, 512
Lsp1109I GCAGC 3 cut(s) 340, 502, 517
LweI GCATC 1 cut(s) 129
MaeI CTAG 1 cut(s) 350
MaeIII GTNAC 2 cut(s) 171, 284
MboII GAAGA 1 cut(s) 530
MhlI GDGCHC 1 cut(s) 406
MmeI TCCRAC 1 cut(s) 492
MnlI CCTC 9 cut(s) 31, 107, 117, 122, 172, 272, 284, 385, 487
MroXI GAANNNNTTC 1 cut(s) 263
MseI TTAA 1 cut(s) 24
MspA1I CMGCKG 1 cut(s) 12
MspI CCGG 3 cut(s) 147, 236, 269
MspR9I CCNGG 3 cut(s) 69, 148, 269
MvaI CCWGG 1 cut(s) 69
MwoI GCNNNNNNNGC 4 cut(s) 164, 200, 401, 493
NciI CCSGG 2 cut(s) 148, 269
NheI GCTAGC 1 cut(s) 349
NlaIII CATG 2 cut(s) 91, 485
NlaIV GGNNCC 3 cut(s) 85, 403, 436
NmeAIII GCCGAG 2 cut(s) 78, 151
NmuCI GTSAC 1 cut(s) 171
NspV TTCGAA 1 cut(s) 44
PagI TCATGA 1 cut(s) 481
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 2 cut(s) 259, 332
PfoI TCCNGGA 1 cut(s) 267
PkrI GCNGC 5 cut(s) 145, 234, 355, 517, 532
Psp6I CCWGG 1 cut(s) 67
PspGI CCWGG 1 cut(s) 67
PspN4I GGNNCC 3 cut(s) 85, 403, 436
PspPI GGNCC 1 cut(s) 271
PstI CTGCAG 1 cut(s) 358
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
SaqAI TTAA 1 cut(s) 24
SatI GCNGC 5 cut(s) 144, 233, 354, 516, 531
Sau96I GGNCC 1 cut(s) 271
ScrFI CCNGG 3 cut(s) 69, 148, 269
SduI GDGCHC 1 cut(s) 406
SetI ASST 9 cut(s) 99, 134, 160, 169, 205, 276, 310, 355, 517
SfaNI GCATC 1 cut(s) 129
SfcI CTRYAG 1 cut(s) 354
SfuI TTCGAA 1 cut(s) 44
SinI GGWCC 1 cut(s) 271
SsiI CCGC 4 cut(s) 12, 118, 144, 233
SspMI CTAG 1 cut(s) 350
StyD4I CCNGG 3 cut(s) 67, 146, 267
TaaI ACNGT 2 cut(s) 422, 473
TaqI TCGA 3 cut(s) 36, 44, 93
TatI WGTACW 1 cut(s) 363
TauI GCSGC 2 cut(s) 146, 235
TfiI GAWTC 2 cut(s) 259, 332
Tru1I TTAA 1 cut(s) 24
Tru9I TTAA 1 cut(s) 24
TscAI CASTG 5 cut(s) 196, 202, 427, 514, 532
TseFI GTSAC 1 cut(s) 171
TseI GCWGC 3 cut(s) 353, 515, 530
Tsp45I GTSAC 1 cut(s) 171
TspRI CASTG 5 cut(s) 196, 202, 427, 514, 532
VpaK11BI GGWCC 1 cut(s) 271
XcmI CCANNNNNNNNNTGG 1 cut(s) 445
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.