Rroxscaffold_5G00366440

Outer envelope pore protein 16-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011960
Physical Location & Seq
Forward (+)
48293600 .. 48295351
1752 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00366440.1

Sequence Viewer

Length: 561 bp
ATGTTGGGTAGCGGGGGCAGCGTCAACACTCATATCGAGGCTTCATCAAAGCCGTCGTCGTTCGAGTTCCCTGGATTCGACAAGGGTGGCATGTTTGACCTCGGCCACCCTCTCCTCAACCGCATCGCCGAGAGCTTTGTCAAAGCCGCTGGCATCGGAGCTATTCAGGCTGTCTCACGTGAGGCTTATTTCACTGCCACTGAAGCTTCAGGACTTGATTCAACTAGTGGAATTCCGCCGGAAATTTCAGGCCACAAAAAGAATCGCTTCCCAAACCTCAGAGGGGATGACAACAGAAAATCTCTTGAAGCTATGGTGAAGCACACTGGAAAGGAATCTTTACAATGGGGGCTAGCTGCAGGGGTGTACTCAGGCCTTACTTATGGACTGAAGGAGGCTCGTGGAGCCCATGACTGGAAAAACAGTGCAGTTGCAGGAGCAATTACCGGTGTGGCTCTGGCACTCACATCCGACAGTTCCTCACATGAGCAGATTGTGCAATGTGCTATCACTGGAGCTGCCATTTCCACTGCTGCAAATCTTCTCTCTGGAATATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

186

Amino Acids

19.27

Weight (kDa)

6.59

Isoelectric Point (pI)

26.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 39 - 176 9.8e-17 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16160 AT4G16160
fragaria_vesca FvH4_4g19460
malus_domestica MD13G1156400.v1.1 MD16G1157300.v1.1
prunus_persica Prupe.1G122200_v2.0.a1 Prupe.1G122200_v2.0.a1
pyrus_communis pycom13g13380
rosa_chinensis RchiOBHm_Chr4g0424511
rosa_laevigata RLG00000007467
rosa_multiflora Rmu_sc0002603.1_g000009 Rmu_sc0003942.1_g000024
rosa_roxburghii Rroxscaffold_5G00366440
rosa_rugosa Rorug04G0198000
rosa_samantha Rh4AG254500 Rh4BG259900 Rh4CG271000 Rh4DG254300
rosa_wichuraiana Rw4G021980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 12, 121, 147, 236
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 2 cut(s) 231, 243
AcuI CTGAAG 3 cut(s) 192, 222, 410
AcvI CACGTG 1 cut(s) 179
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 2 cut(s) 283, 414
AgeI ACCGGT 1 cut(s) 446
AgsI TTSAA 2 cut(s) 222, 308
AhlI ACTAGT 1 cut(s) 224
AjnI CCWGG 1 cut(s) 70
AluBI AGCT 6 cut(s) 135, 161, 206, 311, 356, 518
AluI AGCT 6 cut(s) 135, 161, 206, 311, 356, 518
Alw26I GTCTC 1 cut(s) 178
AoxI GGCC 3 cut(s) 103, 250, 373
ApeKI GCWGC 4 cut(s) 18, 356, 518, 533
ApoI RAATTY 2 cut(s) 231, 243
ArsI GACNNNNNNTTYG 2 cut(s) 41, 73
AsiGI ACCGGT 1 cut(s) 446
Asp700I GAANNNNTTC 1 cut(s) 266
AsuHPI GGTGA 1 cut(s) 328
AsuNHI GCTAGC 1 cut(s) 352
BanII GRGCYC 1 cut(s) 409
BauI CACGAG 1 cut(s) 399
BbrPI CACGTG 1 cut(s) 179
BbvI GCAGC 4 cut(s) 30, 343, 505, 520
BceAI ACGGC 1 cut(s) 37
BciT130I CCWGG 1 cut(s) 72
BcoDI GTCTC 1 cut(s) 178
BcuI ACTAGT 1 cut(s) 224
BfaI CTAG 2 cut(s) 225, 353
BfmI CTRYAG 1 cut(s) 357
BisI GCNGC 5 cut(s) 19, 147, 357, 519, 534
BlsI GCNGC 5 cut(s) 20, 148, 358, 520, 535
Bme1390I CCNGG 1 cut(s) 72
BmiI GGNNCC 1 cut(s) 406
BmrFI CCNGG 1 cut(s) 72
BmsI GCATC 2 cut(s) 132, 162
BmtI GCTAGC 1 cut(s) 356
BpmI CTGGAG 1 cut(s) 534
BsaAI YACGTR 1 cut(s) 179
BsaJI CCNNGG 2 cut(s) 70, 100
BsaWI WCCGGW 1 cut(s) 446
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 414
Bse118I RCCGGY 1 cut(s) 446
Bse1I ACTGG 3 cut(s) 331, 419, 517
Bse3DI GCAATG 1 cut(s) 506
BseBI CCWGG 1 cut(s) 72
BseDI CCNNGG 2 cut(s) 70, 100
BseGI GGATG 2 cut(s) 292, 467
BseLI CCNNNNNNNGG 2 cut(s) 283, 414
BseMI GCAATG 1 cut(s) 506
BseMII CTCAG 2 cut(s) 292, 384
BseNI ACTGG 3 cut(s) 331, 419, 517
BseRI GAGGAG 1 cut(s) 104
BseXI GCAGC 4 cut(s) 30, 343, 505, 520
BsgI GTGCAG 1 cut(s) 447
BshFI GGCC 3 cut(s) 105, 252, 375
BshTI ACCGGT 1 cut(s) 446
BsiSI CCGG 2 cut(s) 239, 447
BslI CCNNNNNNNGG 2 cut(s) 283, 414
BsmAI GTCTC 1 cut(s) 178
BsnI GGCC 3 cut(s) 105, 252, 375
Bsp1286I GDGCHC 1 cut(s) 409
BspACI CCGC 4 cut(s) 12, 121, 147, 236
BspANI GGCC 3 cut(s) 105, 252, 375
BspCNI CTCAG 2 cut(s) 291, 383
BspLI GGNNCC 1 cut(s) 406
BspMAI CTGCAG 1 cut(s) 361
BspOI GCTAGC 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 506
BsrFI RCCGGY 1 cut(s) 446
BsrI ACTGG 3 cut(s) 331, 419, 517
BssAI RCCGGY 1 cut(s) 446
BssECI CCNNGG 2 cut(s) 70, 100
BssSI CACGAG 1 cut(s) 399
Bst2BI CACGAG 1 cut(s) 399
Bst2UI CCWGG 1 cut(s) 72
Bst4CI ACNGT 2 cut(s) 425, 476
BstAPI GCANNNNNTGC 1 cut(s) 496
BstBAI YACGTR 1 cut(s) 179
BstC8I GCNNGC 2 cut(s) 151, 354
BstDEI CTNAG 2 cut(s) 278, 370
BstF5I GGATG 2 cut(s) 292, 467
BstMAI GTCTC 1 cut(s) 178
BstMWI GCNNNNNNNGC 5 cut(s) 18, 167, 203, 404, 496
BstNI CCWGG 1 cut(s) 72
BstNSI RCATGY 1 cut(s) 94
BstSCI CCNGG 1 cut(s) 70
BstSFI CTRYAG 1 cut(s) 357
BstV1I GCAGC 4 cut(s) 30, 343, 505, 520
BsuRI GGCC 3 cut(s) 105, 252, 375
BtgZI GCGATG 1 cut(s) 109
BtsCI GGATG 2 cut(s) 292, 467
BtsI GCAGTG 2 cut(s) 192, 528
BtsIMutI CAGTG 6 cut(s) 192, 198, 324, 430, 510, 528
Cac8I GCNNGC 2 cut(s) 151, 354
Cfr10I RCCGGY 1 cut(s) 446
CseI GACGC 1 cut(s) 10
Csp6I GTAC 1 cut(s) 367
CspAI ACCGGT 1 cut(s) 446
CviAII CATG 3 cut(s) 91, 410, 485
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 2 cut(s) 278, 370
EaeI YGGCCR 1 cut(s) 103
EciI GGCGGA 1 cut(s) 225
Eco147I AGGCCT 1 cut(s) 375
Eco24I GRGCYC 1 cut(s) 409
Eco57I CTGAAG 3 cut(s) 192, 222, 410
Eco72I CACGTG 1 cut(s) 179
EcoRI GAATTC 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 70
EcoT38I GRGCYC 1 cut(s) 409
FaeI CATG 3 cut(s) 94, 413, 488
FaiI YATR 6 cut(s) 33, 92, 314, 384, 411, 486
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FatI CATG 3 cut(s) 90, 409, 484
FauI CCCGC 1 cut(s) 5
Fnu4HI GCNGC 5 cut(s) 19, 147, 357, 519, 534
FokI GGATG 2 cut(s) 299, 454
FriOI GRGCYC 1 cut(s) 409
Fsp4HI GCNGC 5 cut(s) 19, 147, 357, 519, 534
FspBI CTAG 2 cut(s) 225, 353
GluI GCNGC 5 cut(s) 19, 147, 357, 519, 534
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 3 cut(s) 105, 252, 375
HapII CCGG 2 cut(s) 239, 447
HgaI GACGC 1 cut(s) 10
Hin1II CATG 3 cut(s) 94, 413, 488
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HindIII AAGCTT 1 cut(s) 204
HinfI GANTC 4 cut(s) 75, 218, 262, 335
HpaII CCGG 2 cut(s) 239, 447
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 2 cut(s) 25, 367
Hpy188I TCNGA 3 cut(s) 158, 281, 472
Hpy188III TCNNGA 3 cut(s) 210, 305, 549
Hpy8I GTNNAC 2 cut(s) 25, 367
Hpy99I CGWCG 2 cut(s) 58, 61
HpyAV CCTTC 1 cut(s) 385
HpyCH4III ACNGT 2 cut(s) 425, 476
HpyCH4IV ACGT 1 cut(s) 178
HpyCH4V TGCA 5 cut(s) 359, 428, 434, 499, 536
HpyF10VI GCNNNNNNNGC 5 cut(s) 18, 167, 203, 404, 496
HpyF3I CTNAG 2 cut(s) 278, 370
HpySE526I ACGT 1 cut(s) 178
Hsp92II CATG 3 cut(s) 94, 413, 488
LmnI GCTCC 4 cut(s) 158, 404, 437, 515
Lsp1109I GCAGC 4 cut(s) 30, 343, 505, 520
LweI GCATC 2 cut(s) 132, 162
MaeI CTAG 2 cut(s) 225, 353
MaeII ACGT 1 cut(s) 178
MboII GAAGA 1 cut(s) 533
MhlI GDGCHC 1 cut(s) 409
MluCI AATT 3 cut(s) 231, 243, 441
MmeI TCCRAC 1 cut(s) 495
MnlI CCTC 9 cut(s) 31, 110, 120, 125, 175, 275, 287, 388, 490
MroXI GAANNNNTTC 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 2 cut(s) 239, 447
MspR9I CCNGG 1 cut(s) 72
MvaI CCWGG 1 cut(s) 72
MwoI GCNNNNNNNGC 5 cut(s) 18, 167, 203, 404, 496
NheI GCTAGC 1 cut(s) 352
NlaIII CATG 3 cut(s) 94, 413, 488
NlaIV GGNNCC 1 cut(s) 406
NmeAIII GCCGAG 2 cut(s) 81, 154
NspI RCATGY 1 cut(s) 94
PceI AGGCCT 1 cut(s) 375
PdmI GAANNNNTTC 1 cut(s) 266
PfeI GAWTC 4 cut(s) 75, 218, 262, 335
PinAI ACCGGT 1 cut(s) 446
PkrI GCNGC 5 cut(s) 20, 148, 358, 520, 535
PmaCI CACGTG 1 cut(s) 179
PmlI CACGTG 1 cut(s) 179
Ppu21I YACGTR 1 cut(s) 179
Psp6I CCWGG 1 cut(s) 70
PspCI CACGTG 1 cut(s) 179
PspGI CCWGG 1 cut(s) 70
PspN4I GGNNCC 1 cut(s) 406
PstI CTGCAG 1 cut(s) 361
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
SatI GCNGC 5 cut(s) 19, 147, 357, 519, 534
ScrFI CCNGG 1 cut(s) 72
SduI GDGCHC 1 cut(s) 409
SetI ASST 9 cut(s) 102, 137, 163, 181, 208, 279, 313, 358, 520
SfaNI GCATC 2 cut(s) 132, 162
SfcI CTRYAG 1 cut(s) 357
SpeI ACTAGT 1 cut(s) 224
Sse9I AATT 3 cut(s) 231, 243, 441
SseBI AGGCCT 1 cut(s) 375
SsiI CCGC 4 cut(s) 12, 121, 147, 236
SspI AATATT 1 cut(s) 555
SspMI CTAG 2 cut(s) 225, 353
StuI AGGCCT 1 cut(s) 375
StyD4I CCNGG 1 cut(s) 70
TaaI ACNGT 2 cut(s) 425, 476
TaiI ACGT 1 cut(s) 181
TaqI TCGA 3 cut(s) 36, 63, 78
TasI AATT 3 cut(s) 231, 243, 441
TatI WGTACW 1 cut(s) 366
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 4 cut(s) 75, 218, 262, 335
TscAI CASTG 6 cut(s) 199, 205, 331, 430, 517, 535
TseI GCWGC 4 cut(s) 18, 356, 518, 533
TspDTI ATGAA 1 cut(s) 33
TspRI CASTG 6 cut(s) 199, 205, 331, 430, 517, 535
XapI RAATTY 2 cut(s) 231, 243
XceI RCATGY 1 cut(s) 94
XmnI GAANNNNTTC 1 cut(s) 266
XspI CTAG 2 cut(s) 225, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.