MD16G1157300.v1.1

Outer envelope pore protein 16-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011960
Physical Location & Seq
Reverse (-)
12585084 .. 12587610
2527 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1157300.v1.1.491

Sequence Viewer

Length: 834 bp
ATGTTTGTTTATGAAACAGGTCAACAGGGGACAGGTGGCAAACATTTGGAAGAAGTCGCTGGCCACCGAGTTCGCAGTGTGATCACGCAGAAGAAAGACACGTACGTCGGGTTGCATGAGTACGTGTCTACCTCACTTGTTCATTTTCTCTTTCTTCTTCTTTTTCACCGGTGCAATCTCATCACTCATCAGAATAACCTCAAATTTCACTTTGTGTTGAATCTCTTGGTGGAAGAGCAGACAGATAAAACAAATCAAACAGATAATAGTGTGAAGATGTTGAACACGAGCAGCGGCAGTAGTAGTTTGGAAACAAAGCCGTCGTTGCTGCAGGAGCTGCGTAGCTTCGACAAGGGTGGCTTCTCCGACCTCGGCCACCCTCTCCTCAACCGCATCGCCGAGAGCTTCGTCAAAGCTGCTGGGATCGGAGCAATTCAGGCCGTGTCGCGTGAGGCTTATTTCACAGCCACTGAAGGATTCGATTCAAGTACCAATGGTATTCCACCCGAAATCTCAGTCTCAGGCAACAAAAAACAACGATTCCCTGATCTCAGAGGTGAAAACAACAGAAAATCTCTTGAAGCTATGGTGAAGAATACAGGAAAGGAATCCTTACAATGGGGGCTGGCTGCGGGGGTGTATTCAGGTCTCACATATGGCCTGGCAGAGGCTCGTGGAGCTCACGATTGGAAAAACAGTGCAGTTGCGGGAGCAATTACAGGAGTGGCCCTGGCCCTCACATCGGAGGGTTCGTCCCATGAGCAGATTGTGCATGGCGCTATCACCGGAGCTGCCATATCAACCGCTGCAAATCTTCTTTCTGGGATATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

278

Amino Acids

29.84

Weight (kDa)

6.84

Isoelectric Point (pI)

22.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 129 - 267 3.8e-16 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16160 AT4G16160
fragaria_vesca FvH4_4g19460
malus_domestica MD13G1156400.v1.1 MD16G1157300.v1.1
prunus_persica Prupe.1G122200_v2.0.a1 Prupe.1G122200_v2.0.a1
pyrus_communis pycom13g13380
rosa_chinensis RchiOBHm_Chr4g0424511
rosa_laevigata RLG00000007467
rosa_multiflora Rmu_sc0002603.1_g000009 Rmu_sc0003942.1_g000024
rosa_roxburghii Rroxscaffold_5G00366440
rosa_rugosa Rorug04G0198000
rosa_samantha Rh4AG254500 Rh4BG259900 Rh4CG271000 Rh4DG254300
rosa_wichuraiana Rw4G021980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 104
AccI GTMKAC 1 cut(s) 128
AccII CGCG 1 cut(s) 448
AciI CCGC 5 cut(s) 294, 391, 632, 707, 804
AclWI GGATC 1 cut(s) 431
AcoI YGGCCR 2 cut(s) 61, 373
AcsI RAATTY 1 cut(s) 203
AcuI CTGAAG 1 cut(s) 492
AdeI CACNNNGTG 1 cut(s) 214
AfaI GTAC 3 cut(s) 104, 122, 490
AfiI CCNNNNNNNGG 3 cut(s) 618, 667, 742
AflIII ACRYGT 2 cut(s) 99, 123
AgeI ACCGGT 1 cut(s) 168
AgsI TTSAA 4 cut(s) 220, 283, 486, 581
AjnI CCWGG 2 cut(s) 660, 729
AluBI AGCT 7 cut(s) 337, 345, 405, 416, 584, 680, 791
AluI AGCT 7 cut(s) 337, 345, 405, 416, 584, 680, 791
Alw21I GWGCWC 1 cut(s) 682
Alw26I GTCTC 2 cut(s) 523, 653
AlwI GGATC 1 cut(s) 431
AlwNI CAGNNNCTG 2 cut(s) 337, 470
AoxI GGCC 6 cut(s) 61, 373, 438, 658, 726, 732
ApeKI GCWGC 7 cut(s) 291, 328, 337, 416, 629, 791, 806
ApoI RAATTY 1 cut(s) 203
ArsI GACNNNNNNTTYG 2 cut(s) 501, 533
AsiGI ACCGGT 1 cut(s) 168
AspLEI GCGC 1 cut(s) 779
AspS9I GGNCC 2 cut(s) 727, 733
AsuHPI GGTGA 4 cut(s) 158, 569, 601, 775
BalI TGGCCA 1 cut(s) 63
BanII GRGCYC 1 cut(s) 682
BauI CACGAG 2 cut(s) 286, 672
Bbv12I GWGCWC 1 cut(s) 682
BbvI GCAGC 7 cut(s) 303, 315, 324, 403, 616, 778, 793
BceAI ACGGC 2 cut(s) 304, 425
BciT130I CCWGG 2 cut(s) 662, 731
BclI TGATCA 1 cut(s) 81
BcoDI GTCTC 2 cut(s) 523, 653
BfmI CTRYAG 1 cut(s) 329
BfoI RGCGCY 1 cut(s) 780
BisI GCNGC 8 cut(s) 292, 295, 329, 338, 417, 630, 792, 807
BlsI GCNGC 8 cut(s) 293, 296, 330, 339, 418, 631, 793, 808
Bme1390I CCNGG 2 cut(s) 662, 731
BmgT120I GGNCC 2 cut(s) 727, 733
BmrFI CCNGG 2 cut(s) 662, 731
BmsI GCATC 1 cut(s) 402
BsaAI YACGTR 2 cut(s) 102, 124
BsaI GGTCTC 1 cut(s) 653
BsaJI CCNNGG 2 cut(s) 370, 729
BsaWI WCCGGW 2 cut(s) 168, 785
BsaXI ACNNNNNCTCC 2 cut(s) 737, 767
Bsc4I CCNNNNNNNGG 3 cut(s) 618, 667, 742
Bse118I RCCGGY 1 cut(s) 168
BseBI CCWGG 2 cut(s) 662, 731
BseDI CCNNGG 2 cut(s) 370, 729
BseLI CCNNNNNNNGG 3 cut(s) 618, 667, 742
BseMII CTCAG 3 cut(s) 528, 534, 565
BseRI GAGGAG 1 cut(s) 374
BseXI GCAGC 7 cut(s) 303, 315, 324, 403, 616, 778, 793
BseYI CCCAGC 1 cut(s) 419
BsgI GTGCAG 1 cut(s) 720
Bsh1236I CGCG 1 cut(s) 448
BshFI GGCC 6 cut(s) 63, 375, 440, 660, 728, 734
BshTI ACCGGT 1 cut(s) 168
BsiHKAI GWGCWC 1 cut(s) 682
BsiSI CCGG 2 cut(s) 169, 786
BsiWI CGTACG 1 cut(s) 102
BslFI GGGAC 2 cut(s) 43, 739
BslI CCNNNNNNNGG 3 cut(s) 618, 667, 742
BsmAI GTCTC 2 cut(s) 523, 653
BsmFI GGGAC 2 cut(s) 43, 739
BsnI GGCC 6 cut(s) 63, 375, 440, 660, 728, 734
Bso31I GGTCTC 1 cut(s) 653
Bsp1286I GDGCHC 1 cut(s) 682
Bsp143I GATC 3 cut(s) 81, 423, 547
BspACI CCGC 5 cut(s) 294, 391, 632, 707, 804
BspANI GGCC 6 cut(s) 63, 375, 440, 660, 728, 734
BspCNI CTCAG 3 cut(s) 527, 533, 564
BspFNI CGCG 1 cut(s) 448
BspMAI CTGCAG 1 cut(s) 333
BspPI GGATC 1 cut(s) 431
BspQI GCTCTTC 1 cut(s) 228
BspTNI GGTCTC 1 cut(s) 653
BsrFI RCCGGY 1 cut(s) 168
BssAI RCCGGY 1 cut(s) 168
BssECI CCNNGG 2 cut(s) 370, 729
BssMI GATC 3 cut(s) 81, 423, 547
BssSI CACGAG 2 cut(s) 286, 672
Bst2BI CACGAG 2 cut(s) 286, 672
Bst2UI CCWGG 2 cut(s) 662, 731
Bst4CI ACNGT 1 cut(s) 698
Bst6I CTCTTC 1 cut(s) 228
BstAPI GCANNNNNTGC 2 cut(s) 337, 769
BstBAI YACGTR 2 cut(s) 102, 124
BstC8I GCNNGC 2 cut(s) 61, 627
BstDEI CTNAG 3 cut(s) 514, 520, 551
BstENI CCTNNNNNAGG 1 cut(s) 665
BstFNI CGCG 1 cut(s) 448
BstH2I RGCGCY 1 cut(s) 780
BstHHI GCGC 1 cut(s) 779
BstKTI GATC 3 cut(s) 84, 426, 550
BstMAI GTCTC 2 cut(s) 523, 653
BstMBI GATC 3 cut(s) 81, 423, 547
BstMWI GCNNNNNNNGC 6 cut(s) 325, 334, 337, 437, 677, 769
BstNI CCWGG 2 cut(s) 662, 731
BstSCI CCNGG 2 cut(s) 660, 729
BstSFI CTRYAG 1 cut(s) 329
BstUI CGCG 1 cut(s) 448
BstV1I GCAGC 7 cut(s) 303, 315, 324, 403, 616, 778, 793
BsuRI GGCC 6 cut(s) 63, 375, 440, 660, 728, 734
BtgZI GCGATG 1 cut(s) 379
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 3 cut(s) 82, 468, 703
Cac8I GCNNGC 2 cut(s) 61, 627
CaiI CAGNNNCTG 2 cut(s) 337, 470
CfoI GCGC 1 cut(s) 779
Cfr10I RCCGGY 1 cut(s) 168
Cfr13I GGNCC 2 cut(s) 727, 733
Csp6I GTAC 3 cut(s) 103, 121, 489
CspAI ACCGGT 1 cut(s) 168
CviAII CATG 3 cut(s) 116, 758, 773
CviQI GTAC 3 cut(s) 103, 121, 489
DdeI CTNAG 3 cut(s) 514, 520, 551
DpnI GATC 3 cut(s) 83, 425, 549
DpnII GATC 3 cut(s) 81, 423, 547
DraIII CACNNNGTG 1 cut(s) 214
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 2 cut(s) 61, 373
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
Ecl136II GAGCTC 1 cut(s) 680
Eco24I GRGCYC 1 cut(s) 682
Eco31I GGTCTC 1 cut(s) 653
Eco53kI GAGCTC 1 cut(s) 680
Eco57I CTGAAG 1 cut(s) 492
EcoICRI GAGCTC 1 cut(s) 680
EcoNI CCTNNNNNAGG 1 cut(s) 665
EcoRII CCWGG 2 cut(s) 660, 729
EcoT38I GRGCYC 1 cut(s) 682
FaeI CATG 3 cut(s) 119, 761, 776
FaiI YATR 8 cut(s) 12, 117, 587, 655, 657, 759, 774, 797
FalI AAGNNNNNCTT 4 cut(s) 344, 376, 596, 628
FaqI GGGAC 2 cut(s) 43, 739
FatI CATG 3 cut(s) 115, 757, 772
FauI CCCGC 2 cut(s) 625, 700
FauNDI CATATG 1 cut(s) 655
FbaI TGATCA 1 cut(s) 81
FblI GTMKAC 1 cut(s) 128
Fnu4HI GCNGC 8 cut(s) 292, 295, 329, 338, 417, 630, 792, 807
FriOI GRGCYC 1 cut(s) 682
Fsp4HI GCNGC 8 cut(s) 292, 295, 329, 338, 417, 630, 792, 807
GlaI GCGC 1 cut(s) 778
GluI GCNGC 8 cut(s) 292, 295, 329, 338, 417, 630, 792, 807
GsaI CCCAGC 1 cut(s) 423
HaeII RGCGCY 1 cut(s) 780
HaeIII GGCC 6 cut(s) 63, 375, 440, 660, 728, 734
HapII CCGG 2 cut(s) 169, 786
HhaI GCGC 1 cut(s) 779
Hin1II CATG 3 cut(s) 119, 761, 776
Hin6I GCGC 1 cut(s) 777
HinP1I GCGC 1 cut(s) 777
HincII GTYRAC 1 cut(s) 23
HindII GTYRAC 1 cut(s) 23
HinfI GANTC 5 cut(s) 220, 477, 482, 540, 608
HpaII CCGG 2 cut(s) 169, 786
HphI GGTGA 4 cut(s) 158, 569, 601, 775
Hpy166II GTNNAC 2 cut(s) 23, 129
Hpy188I TCNGA 5 cut(s) 192, 367, 428, 554, 745
Hpy188III TCNNGA 2 cut(s) 578, 683
Hpy8I GTNNAC 2 cut(s) 23, 129
Hpy99I CGWCG 2 cut(s) 110, 325
HpyAV CCTTC 1 cut(s) 467
HpyCH4III ACNGT 1 cut(s) 698
HpyCH4IV ACGT 3 cut(s) 101, 105, 123
HpyCH4V TGCA 6 cut(s) 115, 174, 331, 701, 772, 809
HpyF10VI GCNNNNNNNGC 6 cut(s) 325, 334, 337, 437, 677, 769
HpyF3I CTNAG 3 cut(s) 514, 520, 551
HpySE526I ACGT 3 cut(s) 101, 105, 123
Hsp92II CATG 3 cut(s) 119, 761, 776
HspAI GCGC 1 cut(s) 777
Ksp22I TGATCA 1 cut(s) 81
Kzo9I GATC 3 cut(s) 81, 423, 547
LguI GCTCTTC 1 cut(s) 228
LmnI GCTCC 5 cut(s) 334, 428, 677, 710, 788
Lsp1109I GCAGC 7 cut(s) 303, 315, 324, 403, 616, 778, 793
LweI GCATC 1 cut(s) 402
MaeII ACGT 3 cut(s) 101, 105, 123
MalI GATC 3 cut(s) 83, 425, 549
MboI GATC 3 cut(s) 81, 423, 547
MboII GAAGA 8 cut(s) 62, 103, 146, 149, 245, 286, 604, 806
MhlI GDGCHC 1 cut(s) 682
MlsI TGGCCA 1 cut(s) 63
MluCI AATT 3 cut(s) 203, 432, 714
MluNI TGGCCA 1 cut(s) 63
MmeI TCCRAC 1 cut(s) 390
Mox20I TGGCCA 1 cut(s) 63
MscI TGGCCA 1 cut(s) 63
Msp20I TGGCCA 1 cut(s) 63
MspA1I CMGCKG 2 cut(s) 294, 806
MspI CCGG 2 cut(s) 169, 786
MspR9I CCNGG 2 cut(s) 662, 731
MvaI CCWGG 2 cut(s) 662, 731
MvnI CGCG 1 cut(s) 448
MwoI GCNNNNNNNGC 6 cut(s) 325, 334, 337, 437, 677, 769
NdeI CATATG 1 cut(s) 655
NdeII GATC 3 cut(s) 81, 423, 547
NlaIII CATG 3 cut(s) 119, 761, 776
NmeAIII GCCGAG 2 cut(s) 351, 424
PciSI GCTCTTC 1 cut(s) 228
PcsI WCGNNNNNNNCGW 1 cut(s) 749
PfeI GAWTC 5 cut(s) 220, 477, 482, 540, 608
Pfl23II CGTACG 1 cut(s) 102
PinAI ACCGGT 1 cut(s) 168
PkrI GCNGC 8 cut(s) 293, 296, 330, 339, 418, 631, 793, 808
Ppu21I YACGTR 2 cut(s) 102, 124
Psp124BI GAGCTC 1 cut(s) 682
Psp6I CCWGG 2 cut(s) 660, 729
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 2 cut(s) 660, 729
PspLI CGTACG 1 cut(s) 102
PspPI GGNCC 2 cut(s) 727, 733
PstI CTGCAG 1 cut(s) 333
PstNI CAGNNNCTG 2 cut(s) 337, 470
RsaI GTAC 3 cut(s) 104, 122, 490
RsaNI GTAC 3 cut(s) 103, 121, 489
SacI GAGCTC 1 cut(s) 682
SapI GCTCTTC 1 cut(s) 228
SatI GCNGC 8 cut(s) 292, 295, 329, 338, 417, 630, 792, 807
Sau3AI GATC 3 cut(s) 81, 423, 547
Sau96I GGNCC 2 cut(s) 727, 733
ScrFI CCNGG 2 cut(s) 662, 731
SduI GDGCHC 1 cut(s) 682
SfaNI GCATC 1 cut(s) 402
SfcI CTRYAG 1 cut(s) 329
SgrAI CRCCGGYG 1 cut(s) 168
Sse9I AATT 3 cut(s) 203, 432, 714
SsiI CCGC 5 cut(s) 294, 391, 632, 707, 804
SstI GAGCTC 1 cut(s) 682
StyD4I CCNGG 2 cut(s) 660, 729
TaaI ACNGT 1 cut(s) 698
TaiI ACGT 3 cut(s) 104, 108, 126
TaqI TCGA 2 cut(s) 348, 480
TasI AATT 3 cut(s) 203, 432, 714
TauI GCSGC 1 cut(s) 297
TfiI GAWTC 5 cut(s) 220, 477, 482, 540, 608
TscAI CASTG 3 cut(s) 82, 475, 703
TseI GCWGC 7 cut(s) 291, 328, 337, 416, 629, 791, 806
TspDTI ATGAA 2 cut(s) 27, 131
TspRI CASTG 3 cut(s) 82, 475, 703
XagI CCTNNNNNAGG 1 cut(s) 665
XapI RAATTY 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.