Rh4BG259900

Outer envelope pore protein 16-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011960
Physical Location & Seq
Forward (+)
43284638 .. 43285961
1324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG259900.1

Sequence Viewer

Length: 510 bp
ATGTTGGGTAGCGGGGGCAGCGTCAACACTCATATCGAGGCTTCATCAAAGCCGTCGTCGTTCGAGTTCCCTGGATTCGACAAGGGTGGCATGTTCGACCTCGGCCACCCTCTCCTCAACCGCATCGCGGAGAGCTTTGTCAAAGCCGCTGGGATCGGAGCTATTCAGGCTGTCTCACGTGAGGCTTATTTCACTGCCACTGAAGCTTCAGGACTTGATTCAACTAGTGGAATTCCGCCGGAAATTTCAGGCCACAAAAAGAATCGCTTCCCAAACCTCAGAGGGGATAACAACAGAAAATCTCTTGAAGCTATGGTGAAGCACACTGGAAAGGAATCTTTACAATGGGGGCTAGCTGCAGGGGTGTACTCAGGCCTTACTTATGGACTGAAGGAGGCTCGTGGAGCCCATGACTGGGTAATGCTTCATTATTCTTTTTCTTTTTGGGTTGAAGCTTGCAGCGGGCAGACTAGAGATTCTGATACAGTGATACCTACCTATGGTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

169

Amino Acids

18.13

Weight (kDa)

6.59

Isoelectric Point (pI)

30.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 39 - 140 2.7e-07 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16160 AT4G16160
fragaria_vesca FvH4_4g19460
malus_domestica MD13G1156400.v1.1 MD16G1157300.v1.1
prunus_persica Prupe.1G122200_v2.0.a1 Prupe.1G122200_v2.0.a1
pyrus_communis pycom13g13380
rosa_chinensis RchiOBHm_Chr4g0424511
rosa_laevigata RLG00000007467
rosa_multiflora Rmu_sc0002603.1_g000009 Rmu_sc0003942.1_g000024
rosa_roxburghii Rroxscaffold_5G00366440
rosa_rugosa Rorug04G0198000
rosa_samantha Rh4AG254500 Rh4BG259900 Rh4CG271000 Rh4DG254300
rosa_wichuraiana Rw4G021980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 128
AciI CCGC 6 cut(s) 12, 121, 128, 147, 236, 462
AclWI GGATC 1 cut(s) 161
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 2 cut(s) 231, 243
AcuI CTGAAG 3 cut(s) 192, 222, 410
AcvI CACGTG 1 cut(s) 179
AfaI GTAC 2 cut(s) 368, 505
AfiI CCNNNNNNNGG 5 cut(s) 127, 283, 414, 415, 500
AgsI TTSAA 3 cut(s) 222, 308, 452
AhlI ACTAGT 1 cut(s) 224
AjnI CCWGG 1 cut(s) 70
AluBI AGCT 6 cut(s) 135, 161, 206, 311, 356, 455
AluI AGCT 6 cut(s) 135, 161, 206, 311, 356, 455
Alw26I GTCTC 1 cut(s) 178
AlwI GGATC 1 cut(s) 161
AoxI GGCC 3 cut(s) 103, 250, 373
ApeKI GCWGC 3 cut(s) 18, 356, 459
ApoI RAATTY 2 cut(s) 231, 243
ArsI GACNNNNNNTTYG 2 cut(s) 41, 73
Asp700I GAANNNNTTC 1 cut(s) 266
AsuHPI GGTGA 1 cut(s) 328
AsuNHI GCTAGC 1 cut(s) 352
BanII GRGCYC 1 cut(s) 409
BauI CACGAG 1 cut(s) 399
BbrPI CACGTG 1 cut(s) 179
BbvI GCAGC 3 cut(s) 30, 343, 471
BceAI ACGGC 1 cut(s) 37
BciT130I CCWGG 1 cut(s) 72
BcoDI GTCTC 1 cut(s) 178
BcuI ACTAGT 1 cut(s) 224
BfaI CTAG 3 cut(s) 225, 353, 471
BfmI CTRYAG 1 cut(s) 357
BisI GCNGC 4 cut(s) 19, 147, 357, 460
BlsI GCNGC 4 cut(s) 20, 148, 358, 461
Bme1390I CCNGG 1 cut(s) 72
BmiI GGNNCC 1 cut(s) 406
BmrFI CCNGG 1 cut(s) 72
BmrI ACTGGG 1 cut(s) 424
BmsI GCATC 1 cut(s) 132
BmtI GCTAGC 1 cut(s) 356
BmuI ACTGGG 1 cut(s) 424
BsaAI YACGTR 1 cut(s) 179
BsaJI CCNNGG 2 cut(s) 70, 100
Bsc4I CCNNNNNNNGG 5 cut(s) 127, 283, 414, 415, 500
Bse1I ACTGG 2 cut(s) 331, 419
BseBI CCWGG 1 cut(s) 72
BseDI CCNNGG 2 cut(s) 70, 100
BseLI CCNNNNNNNGG 5 cut(s) 127, 283, 414, 415, 500
BseMII CTCAG 2 cut(s) 292, 384
BseNI ACTGG 2 cut(s) 331, 419
BseRI GAGGAG 1 cut(s) 104
BseXI GCAGC 3 cut(s) 30, 343, 471
BseYI CCCAGC 1 cut(s) 149
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 3 cut(s) 105, 252, 375
BsiSI CCGG 1 cut(s) 239
BslI CCNNNNNNNGG 5 cut(s) 127, 283, 414, 415, 500
BsmAI GTCTC 1 cut(s) 178
BsnI GGCC 3 cut(s) 105, 252, 375
Bsp1286I GDGCHC 1 cut(s) 409
Bsp143I GATC 1 cut(s) 153
BspACI CCGC 6 cut(s) 12, 121, 128, 147, 236, 462
BspANI GGCC 3 cut(s) 105, 252, 375
BspCNI CTCAG 2 cut(s) 291, 383
BspFNI CGCG 1 cut(s) 128
BspLI GGNNCC 1 cut(s) 406
BspMAI CTGCAG 1 cut(s) 361
BspOI GCTAGC 1 cut(s) 356
BspPI GGATC 1 cut(s) 161
BsrI ACTGG 2 cut(s) 331, 419
BssECI CCNNGG 2 cut(s) 70, 100
BssMI GATC 1 cut(s) 153
BssSI CACGAG 1 cut(s) 399
Bst2BI CACGAG 1 cut(s) 399
Bst2UI CCWGG 1 cut(s) 72
Bst4CI ACNGT 1 cut(s) 487
BstBAI YACGTR 1 cut(s) 179
BstC8I GCNNGC 3 cut(s) 354, 457, 464
BstDEI CTNAG 2 cut(s) 278, 370
BstFNI CGCG 1 cut(s) 128
BstKTI GATC 1 cut(s) 156
BstMAI GTCTC 1 cut(s) 178
BstMBI GATC 1 cut(s) 153
BstMWI GCNNNNNNNGC 4 cut(s) 18, 167, 203, 404
BstNI CCWGG 1 cut(s) 72
BstNSI RCATGY 1 cut(s) 94
BstSCI CCNGG 1 cut(s) 70
BstSFI CTRYAG 1 cut(s) 357
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 3 cut(s) 30, 343, 471
BsuRI GGCC 3 cut(s) 105, 252, 375
BtgZI GCGATG 1 cut(s) 109
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 4 cut(s) 192, 198, 324, 492
Cac8I GCNNGC 3 cut(s) 354, 457, 464
CseI GACGC 1 cut(s) 10
Csp6I GTAC 2 cut(s) 367, 504
CviAII CATG 2 cut(s) 91, 410
CviQI GTAC 2 cut(s) 367, 504
DdeI CTNAG 2 cut(s) 278, 370
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
EaeI YGGCCR 1 cut(s) 103
EciI GGCGGA 1 cut(s) 225
Eco147I AGGCCT 1 cut(s) 375
Eco24I GRGCYC 1 cut(s) 409
Eco57I CTGAAG 3 cut(s) 192, 222, 410
Eco72I CACGTG 1 cut(s) 179
EcoRI GAATTC 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 70
EcoT38I GRGCYC 1 cut(s) 409
FaeI CATG 2 cut(s) 94, 413
FaiI YATR 6 cut(s) 33, 92, 314, 384, 411, 501
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FatI CATG 2 cut(s) 90, 409
FauI CCCGC 2 cut(s) 5, 455
Fnu4HI GCNGC 4 cut(s) 19, 147, 357, 460
FriOI GRGCYC 1 cut(s) 409
Fsp4HI GCNGC 4 cut(s) 19, 147, 357, 460
FspBI CTAG 3 cut(s) 225, 353, 471
GluI GCNGC 4 cut(s) 19, 147, 357, 460
GsaI CCCAGC 1 cut(s) 153
HaeIII GGCC 3 cut(s) 105, 252, 375
HapII CCGG 1 cut(s) 239
HgaI GACGC 1 cut(s) 10
Hin1II CATG 2 cut(s) 94, 413
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HindIII AAGCTT 2 cut(s) 204, 453
HinfI GANTC 5 cut(s) 75, 218, 262, 335, 476
HpaII CCGG 1 cut(s) 239
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 2 cut(s) 25, 367
Hpy188I TCNGA 3 cut(s) 158, 281, 481
Hpy188III TCNNGA 2 cut(s) 210, 305
Hpy8I GTNNAC 2 cut(s) 25, 367
Hpy99I CGWCG 2 cut(s) 58, 61
HpyAV CCTTC 1 cut(s) 385
HpyCH4III ACNGT 1 cut(s) 487
HpyCH4IV ACGT 1 cut(s) 178
HpyCH4V TGCA 2 cut(s) 359, 459
HpyF10VI GCNNNNNNNGC 4 cut(s) 18, 167, 203, 404
HpyF3I CTNAG 2 cut(s) 278, 370
HpySE526I ACGT 1 cut(s) 178
Hsp92II CATG 2 cut(s) 94, 413
Kzo9I GATC 1 cut(s) 153
LmnI GCTCC 2 cut(s) 158, 404
Lsp1109I GCAGC 3 cut(s) 30, 343, 471
LweI GCATC 1 cut(s) 132
MaeI CTAG 3 cut(s) 225, 353, 471
MaeII ACGT 1 cut(s) 178
MalI GATC 1 cut(s) 155
MboI GATC 1 cut(s) 153
MhlI GDGCHC 1 cut(s) 409
MluCI AATT 2 cut(s) 231, 243
MnlI CCTC 8 cut(s) 31, 110, 120, 125, 175, 275, 287, 388
MroXI GAANNNNTTC 1 cut(s) 266
MseI TTAA 1 cut(s) 508
MspA1I CMGCKG 2 cut(s) 149, 462
MspI CCGG 1 cut(s) 239
MspR9I CCNGG 1 cut(s) 72
MvaI CCWGG 1 cut(s) 72
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 4 cut(s) 18, 167, 203, 404
NdeII GATC 1 cut(s) 153
NheI GCTAGC 1 cut(s) 352
NlaIII CATG 2 cut(s) 94, 413
NlaIV GGNNCC 1 cut(s) 406
NmeAIII GCCGAG 1 cut(s) 81
NspI RCATGY 1 cut(s) 94
PceI AGGCCT 1 cut(s) 375
PdmI GAANNNNTTC 1 cut(s) 266
PfeI GAWTC 5 cut(s) 75, 218, 262, 335, 476
PkrI GCNGC 4 cut(s) 20, 148, 358, 461
PmaCI CACGTG 1 cut(s) 179
PmlI CACGTG 1 cut(s) 179
Ppu21I YACGTR 1 cut(s) 179
Psp6I CCWGG 1 cut(s) 70
PspCI CACGTG 1 cut(s) 179
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 1 cut(s) 70
PspN4I GGNNCC 1 cut(s) 406
PstI CTGCAG 1 cut(s) 361
RsaI GTAC 2 cut(s) 368, 505
RsaNI GTAC 2 cut(s) 367, 504
SaqAI TTAA 1 cut(s) 508
SatI GCNGC 4 cut(s) 19, 147, 357, 460
Sau3AI GATC 1 cut(s) 153
ScrFI CCNGG 1 cut(s) 72
SduI GDGCHC 1 cut(s) 409
SfaNI GCATC 1 cut(s) 132
SfcI CTRYAG 1 cut(s) 357
SpeI ACTAGT 1 cut(s) 224
Sse9I AATT 2 cut(s) 231, 243
SseBI AGGCCT 1 cut(s) 375
SsiI CCGC 6 cut(s) 12, 121, 128, 147, 236, 462
SspMI CTAG 3 cut(s) 225, 353, 471
StuI AGGCCT 1 cut(s) 375
StyD4I CCNGG 1 cut(s) 70
TaaI ACNGT 1 cut(s) 487
TaiI ACGT 1 cut(s) 181
TaqI TCGA 4 cut(s) 36, 63, 78, 96
TasI AATT 2 cut(s) 231, 243
TatI WGTACW 1 cut(s) 366
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 5 cut(s) 75, 218, 262, 335, 476
Tru1I TTAA 1 cut(s) 508
Tru9I TTAA 1 cut(s) 508
TscAI CASTG 4 cut(s) 199, 205, 331, 492
TseI GCWGC 3 cut(s) 18, 356, 459
TspDTI ATGAA 2 cut(s) 33, 416
TspRI CASTG 4 cut(s) 199, 205, 331, 492
XapI RAATTY 2 cut(s) 231, 243
XceI RCATGY 1 cut(s) 94
XmnI GAANNNNTTC 1 cut(s) 266
XspI CTAG 3 cut(s) 225, 353, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.