Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0012728

Physical Location & Seq

Genomic Coordinates Reverse (-)

9306339 .. 9308538

Gene Structure 2200 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G16520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 366 bp

ATGGCAAAAAGCTCGTTCAAGCAAGAGCATGACTTAGAGAAGAGAAGGGCAGAGGCTGCTAGGATTAGAGAGAAGTATCCTGATAGGATTCCGGTGATTGTTGAGAAGGCTGAGAAGAGTGATATACCAACCATCGACAAGAAAAAGTACCTAGTCCCGGCTGATCTGACTGTGGGGCAGTTTGTGTATGTCATTCGCAAAAGAATCAAATTGAGTGCAGAAAAGGCTATCTTCATATTCGTGGACAATGTTCTTCCTCCAGCAGGTGCGCTCATGTCTTCTGTGTACGAAGAGAAAAAGGATGATGATGGGTTCCTCTATGTCACTTACAGCGGAGAAAACACATTTGGATTTGGATCTCCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005776 GO:0006950 GO:0006995 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043562 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051716 GO:0071496

KEGG Pathways

Metabolic & Signaling

ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167

Pfam Domains

Protein Families

Atg8

Protein Analysis

121

Amino Acids

13.76

Weight (kDa)

8.73

Isoelectric Point (pI)

35.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG8	PF02991	14 - 117	3.1e-50	Autophagy protein Atg8 ubiquitin like
APG12	PF04110	40 - 117	2.8e-07	Ubiquitin-like autophagy protein Apg12

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0012728)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16520 AT4G16520
fragaria_vesca	FvH4_5g00060 FvH4_5g00060
malus_domestica	MD00G1114800.v1.1 MD06G1061100.v1.1
prunus_persica	Prupe.5G072400_v2.0.a1 Prupe.5G072400_v2.0.a1 Prupe.5G072400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0202901
rosa_multiflora	Rmu_sc0008159.1_g000018
rosa_roxburghii	Rroxscaffold_3G00254190
rosa_rugosa	Rorug07G0073800 Rorug07G0073800 Rorug07G0073900
rosa_samantha	Rh7AG201500 Rh7DG208500
rosa_wichuraiana	Rw7G017630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	254
Acc36I	ACCTGC	1 cut(s)	254
AciI	CCGC	1 cut(s)	333
AclWI	GGATC	1 cut(s)	364
AfaI	GTAC	2 cut(s)	149, 287
AfiI	CCNNNNNNNGG	2 cut(s)	157, 263
AgsI	TTSAA	1 cut(s)	19
AjuI	GAANNNNNNNTTGG	2 cut(s)	330, 362
AluBI	AGCT	1 cut(s)	12
AluI	AGCT	1 cut(s)	12
AlwI	GGATC	1 cut(s)	364
AlwNI	CAGNNNCTG	1 cut(s)	56
ApeKI	GCWGC	1 cut(s)	56
AspLEI	GCGC	1 cut(s)	271
AsuC2I	CCSGG	1 cut(s)	158
AsuHPI	GGTGA	1 cut(s)	106
BbsI	GAAGAC	1 cut(s)	270
BbvI	GCAGC	1 cut(s)	43
BccI	CCATC	2 cut(s)	140, 302
BcgI	CGANNNNNNTGC	1 cut(s)	28
BciVI	GTATCC	1 cut(s)	87
BcnI	CCSGG	1 cut(s)	158
BfaI	CTAG	2 cut(s)	60, 152
BfuAI	ACCTGC	1 cut(s)	254
BfuI	GTATCC	1 cut(s)	87
BisI	GCNGC	1 cut(s)	57
BlsI	GCNGC	1 cut(s)	58
Bme1390I	CCNGG	1 cut(s)	158
BmiI	GGNNCC	1 cut(s)	314
BmrFI	CCNGG	1 cut(s)	158
BpiI	GAAGAC	1 cut(s)	270
BpmI	CTGGAG	1 cut(s)	243
BpuMI	CCSGG	1 cut(s)	158
BsaBI	GATNNNNATC	1 cut(s)	355
BsaWI	WCCGGW	1 cut(s)	91
Bsc4I	CCNNNNNNNGG	2 cut(s)	157, 263
Bse8I	GATNNNNATC	1 cut(s)	355
BseGI	GGATG	1 cut(s)	307
BseJI	GATNNNNATC	1 cut(s)	355
BseLI	CCNNNNNNNGG	2 cut(s)	157, 263
BseMII	CTCAG	1 cut(s)	102
BseXI	GCAGC	1 cut(s)	43
BsgI	GTGCAG	1 cut(s)	237
BsiSI	CCGG	2 cut(s)	92, 158
BslFI	GGGAC	1 cut(s)	140
BslI	CCNNNNNNNGG	2 cut(s)	157, 263
BsmFI	GGGAC	1 cut(s)	140
Bsp143I	GATC	2 cut(s)	163, 356
BspACI	CCGC	1 cut(s)	333
BspCNI	CTCAG	1 cut(s)	103
BspLI	GGNNCC	1 cut(s)	314
BspMI	ACCTGC	1 cut(s)	254
BspPI	GGATC	1 cut(s)	364
BssMI	GATC	2 cut(s)	163, 356
Bst4CI	ACNGT	1 cut(s)	172
Bst6I	CTCTTC	3 cut(s)	35, 110, 285
BstAPI	GCANNNNNTGC	1 cut(s)	56
BstDEI	CTNAG	2 cut(s)	34, 111
BstENI	CCTNNNNNAGG	1 cut(s)	261
BstF5I	GGATG	1 cut(s)	307
BstHHI	GCGC	1 cut(s)	271
BstKTI	GATC	2 cut(s)	166, 359
BstMBI	GATC	2 cut(s)	163, 356
BstMWI	GCNNNNNNNGC	2 cut(s)	56, 224
BstSCI	CCNGG	1 cut(s)	156
BstV1I	GCAGC	1 cut(s)	43
BstV2I	GAAGAC	1 cut(s)	270
BstX2I	RGATCY	1 cut(s)	356
BstYI	RGATCY	1 cut(s)	356
BsuI	GTATCC	1 cut(s)	87
BtsCI	GGATG	1 cut(s)	307
BveI	ACCTGC	1 cut(s)	254
CaiI	CAGNNNCTG	1 cut(s)	56
CfoI	GCGC	1 cut(s)	271
Csp6I	GTAC	2 cut(s)	148, 286
CviAII	CATG	2 cut(s)	29, 274
CviJI	RGCY	5 cut(s)	12, 56, 110, 161, 227
CviKI_1	RGCY	5 cut(s)	12, 56, 110, 161, 227
CviQI	GTAC	2 cut(s)	148, 286
DdeI	CTNAG	2 cut(s)	34, 111
DpnI	GATC	2 cut(s)	165, 358
DpnII	GATC	2 cut(s)	163, 356
Eam1104I	CTCTTC	3 cut(s)	35, 110, 285
EarI	CTCTTC	3 cut(s)	35, 110, 285
EcoNI	CCTNNNNNAGG	1 cut(s)	261
FaeI	CATG	2 cut(s)	32, 277
FaiI	YATR	7 cut(s)	30, 125, 189, 236, 275, 321, 364
FalI	AAGNNNNNCTT	2 cut(s)	215, 247
FaqI	GGGAC	1 cut(s)	140
FatI	CATG	2 cut(s)	28, 273
Fnu4HI	GCNGC	1 cut(s)	57
FokI	GGATG	1 cut(s)	314
Fsp4HI	GCNGC	1 cut(s)	57
FspBI	CTAG	2 cut(s)	60, 152
GlaI	GCGC	1 cut(s)	270
GluI	GCNGC	1 cut(s)	57
GsuI	CTGGAG	1 cut(s)	243
HapII	CCGG	2 cut(s)	92, 158
HhaI	GCGC	1 cut(s)	271
Hin1II	CATG	2 cut(s)	32, 277
Hin6I	GCGC	1 cut(s)	269
HinP1I	GCGC	1 cut(s)	269
HinfI	GANTC	2 cut(s)	88, 204
HpaII	CCGG	2 cut(s)	92, 158
HphI	GGTGA	1 cut(s)	106
Hpy166II	GTNNAC	2 cut(s)	244, 286
Hpy188I	TCNGA	1 cut(s)	168
Hpy188III	TCNNGA	1 cut(s)	80
Hpy8I	GTNNAC	2 cut(s)	244, 286
HpyAV	CCTTC	2 cut(s)	39, 100
HpyCH4III	ACNGT	1 cut(s)	172
HpyCH4V	TGCA	1 cut(s)	218
HpyF10VI	GCNNNNNNNGC	2 cut(s)	56, 224
HpyF3I	CTNAG	2 cut(s)	34, 111
Hsp92II	CATG	2 cut(s)	32, 277
HspAI	GCGC	1 cut(s)	269
Kzo9I	GATC	2 cut(s)	163, 356
LpnPI	CCDG	5 cut(s)	93, 105, 171, 249, 273
Lsp1109I	GCAGC	1 cut(s)	43
MaeI	CTAG	2 cut(s)	60, 152
MaeIII	GTNAC	1 cut(s)	322
MalI	GATC	2 cut(s)	165, 358
MboI	GATC	2 cut(s)	163, 356
MboII	GAAGA	6 cut(s)	52, 127, 223, 245, 270, 302
MflI	RGATCY	1 cut(s)	356
MluCI	AATT	1 cut(s)	209
MnlI	CCTC	3 cut(s)	46, 267, 326
MslI	CAYNNNNRTG	1 cut(s)	239
MspA1I	CMGCKG	1 cut(s)	333
MspI	CCGG	2 cut(s)	92, 158
MspR9I	CCNGG	1 cut(s)	158
MwoI	GCNNNNNNNGC	2 cut(s)	56, 224
NciI	CCSGG	1 cut(s)	158
NdeII	GATC	2 cut(s)	163, 356
NlaIII	CATG	2 cut(s)	32, 277
NlaIV	GGNNCC	1 cut(s)	314
NmuCI	GTSAC	1 cut(s)	322
PaqCI	CACCTGC	1 cut(s)	254
PfeI	GAWTC	2 cut(s)	88, 204
PkrI	GCNGC	1 cut(s)	58
PspN4I	GGNNCC	1 cut(s)	314
PstNI	CAGNNNCTG	1 cut(s)	56
PsuI	RGATCY	1 cut(s)	356
RsaI	GTAC	2 cut(s)	149, 287
RsaNI	GTAC	2 cut(s)	148, 286
RseI	CAYNNNNRTG	1 cut(s)	239
SatI	GCNGC	1 cut(s)	57
Sau3AI	GATC	2 cut(s)	163, 356
ScrFI	CCNGG	1 cut(s)	158
SetI	ASST	3 cut(s)	14, 153, 268
SmiMI	CAYNNNNRTG	1 cut(s)	239
Sse9I	AATT	1 cut(s)	209
SsiI	CCGC	1 cut(s)	333
SspMI	CTAG	2 cut(s)	60, 152
StyD4I	CCNGG	1 cut(s)	156
TaaI	ACNGT	1 cut(s)	172
TaqI	TCGA	1 cut(s)	135
TasI	AATT	1 cut(s)	209
TfiI	GAWTC	2 cut(s)	88, 204
TseFI	GTSAC	1 cut(s)	322
TseI	GCWGC	1 cut(s)	56
Tsp45I	GTSAC	1 cut(s)	322
TspDTI	ATGAA	1 cut(s)	223
XagI	CCTNNNNNAGG	1 cut(s)	261
XspI	CTAG	2 cut(s)	60, 152

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.