Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0012728

Physical Location & Seq

Genomic Coordinates Forward (+)

24464124 .. 24467262

Gene Structure 3139 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1114800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 501 bp

ATGCAGCCTCCCCACTGTGACTGTATCGTCATCGTCAACCAAACCCCACGGGATTTCGTGGACTTTTCTGATAAAAAATCAAAAGGCTCGCCTTTTCCCTTCTGGCTTCGTCTCAAGACTTCACGAGGTCAAATGGCAAAGAGTTACTTCAAGCAAGAACATGATCTGGAGAAGAGACGGGCAGAGGCTGCCAGGATCAGAGAGAAATATCCGGACAGAATTCCAGTGATTGTGGAGAAGGCAGAAAGAAGTGATATCCCAAACATTGATAAAAAAAAGTACCTTGTCCCGGCTGATCTAACTGTGGGACAATTTGTTTATGTTATCCGCAAAAGGATTAAGTTAAGTGCAGAAAAGGCGATCTTTATATTCGTGGACAATGTACTCCCACCAACAGGTGCAATTATGTCTGCCATATATGAAGAGAAGAAGGACGAAGACGGGTTTCTCTACATCACGTACAGCGGTGAGAACACATTTGGGTATCAGATTCCACTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006950 GO:0006995 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0043562 GO:0050896 GO:0051716 GO:0071496

KEGG Pathways

Metabolic & Signaling

ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167

Pfam Domains

Protein Families

Atg8

Protein Analysis

167

Amino Acids

19.25

Weight (kDa)

9.11

Isoelectric Point (pI)

34.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG8	PF02991	58 - 161	1.1e-50	Autophagy protein Atg8 ubiquitin like
APG12	PF04110	84 - 161	1.5e-06	Ubiquitin-like autophagy protein Apg12

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012728)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16520 AT4G16520
fragaria_vesca	FvH4_5g00060 FvH4_5g00060
malus_domestica	MD00G1114800.v1.1 MD06G1061100.v1.1
prunus_persica	Prupe.5G072400_v2.0.a1 Prupe.5G072400_v2.0.a1 Prupe.5G072400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0202901
rosa_multiflora	Rmu_sc0008159.1_g000018
rosa_roxburghii	Rroxscaffold_3G00254190
rosa_rugosa	Rorug07G0073800 Rorug07G0073800 Rorug07G0073900
rosa_samantha	Rh7AG201500 Rh7DG208500
rosa_wichuraiana	Rw7G017630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	26
AccIII	TCCGGA	1 cut(s)	211
AciI	CCGC	2 cut(s)	328, 465
AclWI	GGATC	1 cut(s)	203
AcsI	RAATTY	1 cut(s)	219
AfaI	GTAC	3 cut(s)	281, 384, 461
AfiI	CCNNNNNNNGG	2 cut(s)	289, 395
AgsI	TTSAA	1 cut(s)	151
AjnI	CCWGG	1 cut(s)	191
Alw26I	GTCTC	2 cut(s)	116, 169
AlwI	GGATC	1 cut(s)	203
AlwNI	CAGNNNCTG	1 cut(s)	188
Aor13HI	TCCGGA	1 cut(s)	211
ApeKI	GCWGC	2 cut(s)	4, 188
ApoI	RAATTY	1 cut(s)	219
AsuC2I	CCSGG	1 cut(s)	290
AsuHPI	GGTGA	1 cut(s)	479
BauI	CACGAG	1 cut(s)	123
BbsI	GAAGAC	1 cut(s)	444
BbvI	GCAGC	2 cut(s)	16, 175
BciT130I	CCWGG	1 cut(s)	193
BcnI	CCSGG	1 cut(s)	290
BcoDI	GTCTC	2 cut(s)	116, 169
BfmI	CTRYAG	1 cut(s)	497
BisI	GCNGC	2 cut(s)	5, 189
BlsI	GCNGC	2 cut(s)	6, 190
Bme1390I	CCNGG	2 cut(s)	193, 290
BmrFI	CCNGG	2 cut(s)	193, 290
BpiI	GAAGAC	1 cut(s)	444
BpmI	CTGGAG	1 cut(s)	188
BpuEI	CTTGAG	1 cut(s)	98
BpuMI	CCSGG	1 cut(s)	290
BsaAI	YACGTR	1 cut(s)	459
BsaJI	CCNNGG	1 cut(s)	47
BsaWI	WCCGGW	1 cut(s)	211
Bsc4I	CCNNNNNNNGG	2 cut(s)	289, 395
Bse1I	ACTGG	1 cut(s)	224
BseAI	TCCGGA	1 cut(s)	211
BseBI	CCWGG	1 cut(s)	193
BseDI	CCNNGG	1 cut(s)	47
BseLI	CCNNNNNNNGG	2 cut(s)	289, 395
BseNI	ACTGG	1 cut(s)	224
BseXI	GCAGC	2 cut(s)	16, 175
BsgI	GTGCAG	1 cut(s)	369
BsiSI	CCGG	2 cut(s)	212, 290
BslFI	GGGAC	2 cut(s)	272, 321
BslI	CCNNNNNNNGG	2 cut(s)	289, 395
BsmAI	GTCTC	2 cut(s)	116, 169
BsmBI	CGTCTC	2 cut(s)	116, 169
BsmFI	GGGAC	2 cut(s)	272, 321
Bsp13I	TCCGGA	1 cut(s)	211
Bsp143I	GATC	4 cut(s)	163, 195, 295, 360
BspACI	CCGC	2 cut(s)	328, 465
BspEI	TCCGGA	1 cut(s)	211
BspPI	GGATC	1 cut(s)	203
BsrI	ACTGG	1 cut(s)	224
BssECI	CCNNGG	1 cut(s)	47
BssMI	GATC	4 cut(s)	163, 195, 295, 360
BssSI	CACGAG	1 cut(s)	123
Bst2BI	CACGAG	1 cut(s)	123
Bst2UI	CCWGG	1 cut(s)	193
Bst4CI	ACNGT	4 cut(s)	17, 23, 304, 498
Bst6I	CTCTTC	2 cut(s)	167, 417
BstAPI	GCANNNNNTGC	1 cut(s)	188
BstBAI	YACGTR	1 cut(s)	459
BstC8I	GCNNGC	1 cut(s)	89
BstDSI	CCRYGG	1 cut(s)	47
BstKTI	GATC	4 cut(s)	166, 198, 298, 363
BstMAI	GTCTC	2 cut(s)	116, 169
BstMBI	GATC	4 cut(s)	163, 195, 295, 360
BstMWI	GCNNNNNNNGC	2 cut(s)	188, 356
BstNI	CCWGG	1 cut(s)	193
BstSCI	CCNGG	2 cut(s)	191, 288
BstSFI	CTRYAG	1 cut(s)	497
BstV1I	GCAGC	2 cut(s)	16, 175
BstV2I	GAAGAC	1 cut(s)	444
BtgI	CCRYGG	1 cut(s)	47
BtsIMutI	CAGTG	3 cut(s)	13, 231, 494
Cac8I	GCNNGC	1 cut(s)	89
CaiI	CAGNNNCTG	1 cut(s)	188
Csp6I	GTAC	3 cut(s)	280, 383, 460
CviAII	CATG	1 cut(s)	161
CviJI	RGCY	5 cut(s)	7, 87, 106, 188, 293
CviKI_1	RGCY	5 cut(s)	7, 87, 106, 188, 293
CviQI	GTAC	3 cut(s)	280, 383, 460
DpnI	GATC	4 cut(s)	165, 197, 297, 362
DpnII	GATC	4 cut(s)	163, 195, 295, 360
DrdI	GACNNNNNNGTC	1 cut(s)	26
DseDI	GACNNNNNNGTC	1 cut(s)	26
Eam1104I	CTCTTC	2 cut(s)	167, 417
EarI	CTCTTC	2 cut(s)	167, 417
Eco32I	GATATC	1 cut(s)	256
EcoRI	GAATTC	1 cut(s)	219
EcoRII	CCWGG	1 cut(s)	191
EcoRV	GATATC	1 cut(s)	256
Esp3I	CGTCTC	2 cut(s)	116, 169
FaeI	CATG	1 cut(s)	164
FaiI	YATR	7 cut(s)	162, 321, 368, 407, 416, 418, 420
FalI	AAGNNNNNCTT	4 cut(s)	131, 163, 347, 379
FaqI	GGGAC	2 cut(s)	272, 321
FatI	CATG	1 cut(s)	160
Fnu4HI	GCNGC	2 cut(s)	5, 189
Fsp4HI	GCNGC	2 cut(s)	5, 189
GluI	GCNGC	2 cut(s)	5, 189
GsuI	CTGGAG	1 cut(s)	188
HapII	CCGG	2 cut(s)	212, 290
Hin1II	CATG	1 cut(s)	164
HincII	GTYRAC	1 cut(s)	37
HindII	GTYRAC	1 cut(s)	37
HinfI	GANTC	1 cut(s)	490
HpaII	CCGG	2 cut(s)	212, 290
HphI	GGTGA	1 cut(s)	479
Hpy166II	GTNNAC	3 cut(s)	37, 61, 376
Hpy188I	TCNGA	3 cut(s)	70, 200, 489
Hpy188III	TCNNGA	4 cut(s)	115, 123, 167, 212
Hpy8I	GTNNAC	3 cut(s)	37, 61, 376
HpyAV	CCTTC	3 cut(s)	109, 232, 424
HpyCH4III	ACNGT	4 cut(s)	17, 23, 304, 498
HpyCH4IV	ACGT	1 cut(s)	458
HpyCH4V	TGCA	3 cut(s)	4, 350, 401
HpyF10VI	GCNNNNNNNGC	2 cut(s)	188, 356
HpySE526I	ACGT	1 cut(s)	458
Hsp92II	CATG	1 cut(s)	164
Kpn2I	TCCGGA	1 cut(s)	211
Kzo9I	GATC	4 cut(s)	163, 195, 295, 360
LpnPI	CCDG	8 cut(s)	88, 152, 178, 205, 225, 237, 303, 381
Lsp1109I	GCAGC	2 cut(s)	16, 175
MaeII	ACGT	1 cut(s)	458
MaeIII	GTNAC	2 cut(s)	17, 143
MalI	GATC	4 cut(s)	165, 197, 297, 362
MboI	GATC	4 cut(s)	163, 195, 295, 360
MboII	GAAGA	4 cut(s)	184, 434, 439, 449
MluCI	AATT	3 cut(s)	219, 311, 402
MnlI	CCTC	3 cut(s)	18, 119, 178
MroI	TCCGGA	1 cut(s)	211
MseI	TTAA	2 cut(s)	339, 344
MspA1I	CMGCKG	1 cut(s)	465
MspI	CCGG	2 cut(s)	212, 290
MspR9I	CCNGG	2 cut(s)	193, 290
MvaI	CCWGG	1 cut(s)	193
MwoI	GCNNNNNNNGC	2 cut(s)	188, 356
NciI	CCSGG	1 cut(s)	290
NdeII	GATC	4 cut(s)	163, 195, 295, 360
NlaIII	CATG	1 cut(s)	164
NmuCI	GTSAC	1 cut(s)	17
PfeI	GAWTC	1 cut(s)	490
PkrI	GCNGC	2 cut(s)	6, 190
Ppu21I	YACGTR	1 cut(s)	459
Psp6I	CCWGG	1 cut(s)	191
PspGI	CCWGG	1 cut(s)	191
PstNI	CAGNNNCTG	1 cut(s)	188
RsaI	GTAC	3 cut(s)	281, 384, 461
RsaNI	GTAC	3 cut(s)	280, 383, 460
SaqAI	TTAA	2 cut(s)	339, 344
SatI	GCNGC	2 cut(s)	5, 189
Sau3AI	GATC	4 cut(s)	163, 195, 295, 360
ScrFI	CCNGG	2 cut(s)	193, 290
SetI	ASST	4 cut(s)	130, 285, 400, 461
SfcI	CTRYAG	1 cut(s)	497
SmlI	CTYRAG	1 cut(s)	113
SmoI	CTYRAG	1 cut(s)	113
Sse9I	AATT	3 cut(s)	219, 311, 402
SsiI	CCGC	2 cut(s)	328, 465
StyD4I	CCNGG	2 cut(s)	191, 288
TaaI	ACNGT	4 cut(s)	17, 23, 304, 498
TaiI	ACGT	1 cut(s)	461
TasI	AATT	3 cut(s)	219, 311, 402
TatI	WGTACW	1 cut(s)	382
TfiI	GAWTC	1 cut(s)	490
Tru1I	TTAA	2 cut(s)	339, 344
Tru9I	TTAA	2 cut(s)	339, 344
TscAI	CASTG	3 cut(s)	20, 231, 501
TseFI	GTSAC	1 cut(s)	17
TseI	GCWGC	2 cut(s)	4, 188
Tsp45I	GTSAC	1 cut(s)	17
TspDTI	ATGAA	1 cut(s)	435
TspRI	CASTG	3 cut(s)	20, 231, 501
XapI	RAATTY	1 cut(s)	219

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.