Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0012728

Physical Location & Seq

Genomic Coordinates Forward (+)

9951744 .. 9953797

Gene Structure 2054 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1061100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 348 bp

ATGATCTGGAAGAGACGGGCAGAGGCTGCTAGGATCAGAGATAAATACCCAGATAGAATTCCAGTGATTGTGGAGAAGGTAGAAAGAAGTGATATCCCAAACATTGATAAGAAAAAGTACCTTGTCCCGGCCGATCTAACTGTGGGACAATTTGTTTATGTTATCCGCAAAAGGATCAAGTTAAGTGCAGAAAAGGCAATCTTTATATTTGTGGACAATGTGCTCCCACCAACAGGTGCAATTATGTCTGCCATATATGAAGAGAAGAAGGATGAAGACGGGTTTCTCTATGTCACGTACAGCGGTGAGAATACATTTGGTTATCAGATTCCACTGTGTAGCCCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006950 GO:0006995 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0043562 GO:0050896 GO:0051716 GO:0071496

KEGG Pathways

Metabolic & Signaling

ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167

Pfam Domains

Protein Families

Atg8

Protein Analysis

116

Amino Acids

13.27

Weight (kDa)

8.97

Isoelectric Point (pI)

35.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG8	PF02991	4 - 107	5.1e-51	Autophagy protein Atg8 ubiquitin like
APG12	PF04110	30 - 107	4.5e-07	Ubiquitin-like autophagy protein Apg12

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012728)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16520 AT4G16520
fragaria_vesca	FvH4_5g00060 FvH4_5g00060
malus_domestica	MD00G1114800.v1.1 MD06G1061100.v1.1
prunus_persica	Prupe.5G072400_v2.0.a1 Prupe.5G072400_v2.0.a1 Prupe.5G072400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0202901
rosa_multiflora	Rmu_sc0008159.1_g000018
rosa_roxburghii	Rroxscaffold_3G00254190
rosa_rugosa	Rorug07G0073800 Rorug07G0073800 Rorug07G0073900
rosa_samantha	Rh7AG201500 Rh7DG208500
rosa_wichuraiana	Rw7G017630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	2 cut(s)	166, 303
AclWI	GGATC	2 cut(s)	41, 182
AcoI	YGGCCR	1 cut(s)	129
AcsI	RAATTY	1 cut(s)	57
AfaI	GTAC	2 cut(s)	119, 299
AfiI	CCNNNNNNNGG	2 cut(s)	127, 233
Alw21I	GWGCWC	1 cut(s)	225
Alw26I	GTCTC	1 cut(s)	7
AlwI	GGATC	2 cut(s)	41, 182
AlwNI	CAGNNNCTG	1 cut(s)	26
AoxI	GGCC	1 cut(s)	129
ApeKI	GCWGC	1 cut(s)	26
ApoI	RAATTY	1 cut(s)	57
AsuC2I	CCSGG	1 cut(s)	128
AsuHPI	GGTGA	1 cut(s)	317
BbsI	GAAGAC	1 cut(s)	282
Bbv12I	GWGCWC	1 cut(s)	225
BbvI	GCAGC	1 cut(s)	13
BcnI	CCSGG	1 cut(s)	128
BcoDI	GTCTC	1 cut(s)	7
BfaI	CTAG	1 cut(s)	30
BisI	GCNGC	1 cut(s)	27
BlsI	GCNGC	1 cut(s)	28
Bme1390I	CCNGG	1 cut(s)	128
BmrFI	CCNGG	1 cut(s)	128
BpiI	GAAGAC	1 cut(s)	282
BpuMI	CCSGG	1 cut(s)	128
BsaAI	YACGTR	1 cut(s)	297
Bsc4I	CCNNNNNNNGG	2 cut(s)	127, 233
Bse1I	ACTGG	1 cut(s)	62
BseGI	GGATG	1 cut(s)	277
BseLI	CCNNNNNNNGG	2 cut(s)	127, 233
BseNI	ACTGG	1 cut(s)	62
BseX3I	CGGCCG	1 cut(s)	129
BseXI	GCAGC	1 cut(s)	13
BsgI	GTGCAG	1 cut(s)	207
Bsh1285I	CGRYCG	1 cut(s)	132
BshFI	GGCC	1 cut(s)	131
BsiEI	CGRYCG	1 cut(s)	132
BsiHKAI	GWGCWC	1 cut(s)	225
BsiSI	CCGG	1 cut(s)	128
BslFI	GGGAC	2 cut(s)	110, 159
BslI	CCNNNNNNNGG	2 cut(s)	127, 233
BsmAI	GTCTC	1 cut(s)	7
BsmBI	CGTCTC	1 cut(s)	7
BsmFI	GGGAC	2 cut(s)	110, 159
BsnI	GGCC	1 cut(s)	131
Bsp1286I	GDGCHC	1 cut(s)	225
Bsp143I	GATC	4 cut(s)	3, 33, 133, 174
BspACI	CCGC	2 cut(s)	166, 303
BspANI	GGCC	1 cut(s)	131
BspPI	GGATC	2 cut(s)	41, 182
BsrI	ACTGG	1 cut(s)	62
BssMI	GATC	4 cut(s)	3, 33, 133, 174
Bst4CI	ACNGT	2 cut(s)	142, 336
Bst6I	CTCTTC	2 cut(s)	5, 255
BstAPI	GCANNNNNTGC	1 cut(s)	26
BstBAI	YACGTR	1 cut(s)	297
BstF5I	GGATG	1 cut(s)	277
BstKTI	GATC	4 cut(s)	6, 36, 136, 177
BstMAI	GTCTC	1 cut(s)	7
BstMBI	GATC	4 cut(s)	3, 33, 133, 174
BstMCI	CGRYCG	1 cut(s)	132
BstMWI	GCNNNNNNNGC	2 cut(s)	26, 194
BstSCI	CCNGG	1 cut(s)	126
BstV1I	GCAGC	1 cut(s)	13
BstV2I	GAAGAC	1 cut(s)	282
BstZI	CGGCCG	1 cut(s)	129
BsuRI	GGCC	1 cut(s)	131
BtsCI	GGATG	1 cut(s)	277
BtsIMutI	CAGTG	2 cut(s)	69, 332
CaiI	CAGNNNCTG	1 cut(s)	26
Csp6I	GTAC	2 cut(s)	118, 298
CviJI	RGCY	3 cut(s)	26, 131, 342
CviKI_1	RGCY	3 cut(s)	26, 131, 342
CviQI	GTAC	2 cut(s)	118, 298
DpnI	GATC	4 cut(s)	5, 35, 135, 176
DpnII	GATC	4 cut(s)	3, 33, 133, 174
EaeI	YGGCCR	1 cut(s)	129
EagI	CGGCCG	1 cut(s)	129
Eam1104I	CTCTTC	2 cut(s)	5, 255
EarI	CTCTTC	2 cut(s)	5, 255
EclXI	CGGCCG	1 cut(s)	129
Eco32I	GATATC	1 cut(s)	94
Eco52I	CGGCCG	1 cut(s)	129
EcoRI	GAATTC	1 cut(s)	57
EcoRV	GATATC	1 cut(s)	94
Esp3I	CGTCTC	1 cut(s)	7
FaiI	YATR	7 cut(s)	159, 206, 245, 254, 256, 258, 291
FalI	AAGNNNNNCTT	2 cut(s)	185, 217
FaqI	GGGAC	2 cut(s)	110, 159
Fnu4HI	GCNGC	1 cut(s)	27
FokI	GGATG	1 cut(s)	284
Fsp4HI	GCNGC	1 cut(s)	27
FspBI	CTAG	1 cut(s)	30
GluI	GCNGC	1 cut(s)	27
HaeIII	GGCC	1 cut(s)	131
HapII	CCGG	1 cut(s)	128
HinfI	GANTC	1 cut(s)	328
HpaII	CCGG	1 cut(s)	128
HphI	GGTGA	1 cut(s)	317
Hpy166II	GTNNAC	1 cut(s)	214
Hpy188I	TCNGA	2 cut(s)	38, 327
Hpy188III	TCNNGA	1 cut(s)	7
Hpy8I	GTNNAC	1 cut(s)	214
HpyAV	CCTTC	2 cut(s)	70, 262
HpyCH4III	ACNGT	2 cut(s)	142, 336
HpyCH4IV	ACGT	1 cut(s)	296
HpyCH4V	TGCA	2 cut(s)	188, 239
HpyF10VI	GCNNNNNNNGC	2 cut(s)	26, 194
HpySE526I	ACGT	1 cut(s)	296
Kzo9I	GATC	4 cut(s)	3, 33, 133, 174
LmnI	GCTCC	1 cut(s)	228
LpnPI	CCDG	4 cut(s)	63, 75, 141, 219
Lsp1109I	GCAGC	1 cut(s)	13
MaeI	CTAG	1 cut(s)	30
MaeII	ACGT	1 cut(s)	296
MaeIII	GTNAC	1 cut(s)	292
MalI	GATC	4 cut(s)	5, 35, 135, 176
MboI	GATC	4 cut(s)	3, 33, 133, 174
MboII	GAAGA	4 cut(s)	22, 272, 277, 287
MhlI	GDGCHC	1 cut(s)	225
MluCI	AATT	3 cut(s)	57, 149, 240
MnlI	CCTC	1 cut(s)	16
MseI	TTAA	1 cut(s)	182
MspA1I	CMGCKG	1 cut(s)	303
MspI	CCGG	1 cut(s)	128
MspR9I	CCNGG	1 cut(s)	128
MwoI	GCNNNNNNNGC	2 cut(s)	26, 194
NciI	CCSGG	1 cut(s)	128
NdeII	GATC	4 cut(s)	3, 33, 133, 174
NmuCI	GTSAC	1 cut(s)	292
PfeI	GAWTC	1 cut(s)	328
PkrI	GCNGC	1 cut(s)	28
Ppu21I	YACGTR	1 cut(s)	297
PstNI	CAGNNNCTG	1 cut(s)	26
RsaI	GTAC	2 cut(s)	119, 299
RsaNI	GTAC	2 cut(s)	118, 298
SaqAI	TTAA	1 cut(s)	182
SatI	GCNGC	1 cut(s)	27
Sau3AI	GATC	4 cut(s)	3, 33, 133, 174
ScrFI	CCNGG	1 cut(s)	128
SduI	GDGCHC	1 cut(s)	225
SetI	ASST	4 cut(s)	81, 123, 238, 299
Sse9I	AATT	3 cut(s)	57, 149, 240
SsiI	CCGC	2 cut(s)	166, 303
SspMI	CTAG	1 cut(s)	30
StyD4I	CCNGG	1 cut(s)	126
TaaI	ACNGT	2 cut(s)	142, 336
TaiI	ACGT	1 cut(s)	299
TasI	AATT	3 cut(s)	57, 149, 240
TfiI	GAWTC	1 cut(s)	328
Tru1I	TTAA	1 cut(s)	182
Tru9I	TTAA	1 cut(s)	182
TscAI	CASTG	2 cut(s)	69, 339
TseFI	GTSAC	1 cut(s)	292
TseI	GCWGC	1 cut(s)	26
Tsp45I	GTSAC	1 cut(s)	292
TspDTI	ATGAA	2 cut(s)	273, 288
TspRI	CASTG	2 cut(s)	69, 339
XapI	RAATTY	1 cut(s)	57
XspI	CTAG	1 cut(s)	30

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.