AT4G20270
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012854
Physical Location & Seq
Forward (+)
10949573 .. 10953113
3541 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G20270.1

Sequence Viewer

Length: 2979 bp
ATGGCAGACAAGATCTTCACTTTCTTCCTAATCTTGTCTTCGATCTCTCCTCTCTTATGCTCTTCTTTGATCTCACCTCTTAATCTCTCACTTATTAGACAAGCAAATGTCCTTATCTCTCTAAAGCAAAGTTTTGATTCCTATGATCCTTCTCTTGATTCATGGAACATTCCAAATTTCAACTCTCTATGTTCTTGGACTGGTGTTTCTTGTGACAACTTGAATCAGTCTATTACTCGTCTAGACCTATCTAATCTCAACATCTCCGGCACTATCTCTCCGGAAATATCTCGTCTTTCGCCGTCACTTGTTTTTCTTGACATTTCTTCTAACAGTTTCTCCGGTGAGCTTCCTAAAGAGATCTATGAGCTCTCAGGCCTCGAAGTGTTAAACATCTCTAGCAATGTTTTTGAAGGAGAGCTGGAGACACGTGGGTTCAGTCAAATGACTCAGCTTGTGACTCTTGACGCTTACGACAACAGCTTCAACGGATCACTTCCTCTGAGTCTAACCACACTCACTCGTCTCGAGCACTTAGATCTTGGAGGAAACTACTTCGACGGTGAGATCCCTAGAAGCTATGGAAGTTTCTTGAGTCTCAAGTTTCTTTCTTTATCTGGTAATGATCTCCGTGGGAGAATCCCTAACGAGCTAGCGAACATCACGACTTTGGTACAGCTTTACTTAGGTTACTACAACGATTACCGCGGTGGGATACCTGCAGATTTCGGGAGATTGATCAATCTTGTTCATTTGGATTTAGCTAATTGCAGCTTGAAAGGATCAATTCCTGCAGAATTGGGGAATCTCAAGAACTTGGAGGTTCTGTTTCTTCAGACCAATGAGCTTACAGGCTCTGTTCCTCGAGAGTTAGGGAACATGACAAGCCTCAAGACTCTTGATCTCTCCAACAACTTTCTTGAAGGAGAGATTCCTCTAGAGCTATCTGGACTTCAAAAGCTTCAGTTGTTTAACCTCTTCTTCAACAGACTACACGGCGAGATCCCTGAGTTCGTATCTGAGCTTCCTGATCTGCAAATACTCAAGCTTTGGCACAACAATTTCACCGGAAAGATTCCTTCGAAACTCGGATCAAACGGGAACTTGATCGAGATCGATTTGTCTACCAATAAACTCACAGGTTTGATCCCTGAGTCACTCTGTTTCGGAAGAAGACTAAAGATTCTCATTCTCTTCAACAACTTCTTGTTCGGTCCTCTCCCTGAAGATCTTGGCCAATGTGAACCGCTATGGAGATTCCGTCTCGGACAGAACTTTCTGACAAGTAAGTTGCCAAAGGGTTTGATTTATTTGCCGAATCTTTCGCTTCTTGAGCTTCAAAACAACTTTTTGACTGGAGAAATCCCCGAAGAAGAGGCGGGAAATGCGCAGTTTTCGAGCCTTACTCAGATCAATCTGTCCAACAACAGGTTATCCGGACCGATTCCTGGTTCAATCAGAAACCTCAGAAGCCTTCAGATTCTTCTTCTCGGTGCAAACCGGTTATCGGGACAGATCCCTGGCGAAATCGGAAGTTTGAAGAGTCTTCTCAAGATTGACATGAGCAGAAACAACTTCTCAGGCAAGTTTCCTCCTGAGTTTGGTGATTGCATGTCACTCACATATTTAGATTTGAGTCACAACCAGATTTCCGGTCAGATTCCGGTTCAGATATCGCAGATTCGGATTCTAAACTATCTGAATGTTTCTTGGAATTCCTTTAACCAAAGCCTTCCCAACGAACTCGGATACATGAAGAGTTTAACATCAGCAGATTTCTCACACAACAACTTCTCCGGTTCAGTACCAACTTCAGGGCAATTCTCTTACTTCAACAACACGTCATTCCTTGGAAACCCTTTTCTCTGTGGATTTTCTTCAAACCCTTGCAACGGTTCCCAAAACCAATCTCAATCTCAGCTACTTAACCAGAACAACGCAAGATCCCGAGGTGAAATCTCCGCAAAATTCAAGTTGTTCTTCGGGTTAGGCCTACTAGGGTTTTTCTTGGTGTTCGTCGTTTTAGCTGTGGTCAAGAATAGGAGAATGAGAAAGAACAACCCGAATTTATGGAAGCTTATAGGGTTTCAGAAGCTCGGTTTCAGAAGCGAACACATATTAGAATGTGTTAAAGAGAACCATGTGATTGGGAAAGGCGGACGAGGGATTGTCTACAAAGGGGTAATGCCAAACGGAGAAGAAGTTGCAGTCAAGAAGCTCTTAACCATAACCAAAGGATCATCTCATGACAACGGTTTAGCCGCAGAGATTCAGACATTAGGTAGAATCAGACACAGAAACATAGTGAGATTGCTCGCTTTTTGTTCAAACAAAGACGTGAATCTCCTTGTTTACGAGTATATGCCTAATGGTAGCCTCGGAGAAGTCTTGCACGGGAAAGCTGGAGTGTTTTTGAAATGGGAAACACGGTTGCAAATAGCGTTGGAAGCGGCTAAGGGGTTGTGTTATCTTCACCATGATTGCTCGCCACTTATAATCCACCGTGATGTGAAGTCAAACAACATCTTGTTGGGTCCTGAGTTTGAAGCTCATGTTGCTGATTTTGGGCTTGCTAAGTTTATGATGCAAGACAATGGAGCTTCCGAGTGCATGTCCTCGATCGCTGGCTCGTACGGCTACATCGCTCCAGAATATGCATATACACTGAGAATAGACGAGAAGAGCGATGTGTACAGCTTCGGAGTAGTGTTATTGGAGCTGATTACGGGTCGAAAACCAGTAGATAATTTTGGGGAAGAAGGGATAGACATTGTGCAATGGTCAAAGATCCAAACAAACTGTAACAGACAAGGTGTGGTGAAGATCATTGACCAGAGATTGAGCAATATTCCATTAGCAGAGGCCATGGAACTGTTCTTTGTGGCAATGCTATGTGTGCAAGAACATAGTGTTGAGAGACCGACCATGAGAGAGGTTGTCCAGATGATCTCTCAGGCTAAACAGCCTAATACTTTCTAA

Protein Analysis

992

Amino Acids

110.28

Weight (kDa)

6.12

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 72 3.5e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 285 - 447 1e-05 Leucine-rich repeat region
Pkinase PF00069 712 - 981 3.5e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 712 - 982 1.6e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012854)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20270
fragaria_vesca FvH4_3g13410
malus_domestica MD05G1241900.v1.1 MD10G1223800.v1.1
prunus_persica Prupe.4G121500_v2.0.a1
pyrus_communis pycom05g21850 pycom10g18770 pycom10g18780
rosa_chinensis RchiOBHm_Chr5g0022141
rosa_laevigata RLG00000032647
rosa_multiflora Rmu_sc0000491.1_g000007
rosa_roxburghii Rroxscaffold_1G00056790
rosa_rugosa Rorug05G0071500.1
rosa_samantha Rh5AG159600 Rh5BG159100 Rh5CG173300 Rh5DG159100
rosa_wichuraiana Rw5G014250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2495
Acc16I TGCGCA 1 cut(s) 1389
Acc36I ACCTGC 1 cut(s) 727
AccI GTMKAC 2 cut(s) 1124, 2172
AccII CGCG 1 cut(s) 708
AccIII TCCGGA 2 cut(s) 280, 1436
AciI CCGC 8 cut(s) 706, 708, 1247, 1379, 1962, 2157, 2262, 2450
AcoI YGGCCR 1 cut(s) 1234
AcsI RAATTY 4 cut(s) 175, 1714, 1967, 2065
AcuI CTGAAG 5 cut(s) 818, 947, 1245, 1460, 1797
AcvI CACGTG 1 cut(s) 431
AfaI GTAC 4 cut(s) 675, 1806, 2633, 2693
AfiI CCNNNNNNNGG 6 cut(s) 1428, 1448, 1507, 1601, 1814, 1892
AflIII ACRYGT 2 cut(s) 428, 1839
AgeI ACCGGT 1 cut(s) 1500
AjiI CACGTC 2 cut(s) 1842, 2338
AjnI CCWGG 2 cut(s) 1447, 1519
Alw21I GWGCWC 2 cut(s) 372, 534
Alw26I GTCTC 5 cut(s) 419, 530, 602, 1268, 2911
AlwNI CAGNNNCTG 1 cut(s) 857
Ama87I CYCGRG 3 cut(s) 527, 864, 1947
Aor13HI TCCGGA 2 cut(s) 280, 1436
AoxI GGCC 4 cut(s) 376, 1234, 1990, 2862
ApeKI GCWGC 1 cut(s) 771
ApoI RAATTY 4 cut(s) 175, 1714, 1967, 2065
AsiGI ACCGGT 1 cut(s) 1500
AspLEI GCGC 1 cut(s) 1390
AspS9I GGNCC 3 cut(s) 1214, 1439, 2534
AsuHPI GGTGA 8 cut(s) 66, 356, 575, 1057, 1616, 1964, 2465, 2830
AsuII TTCGAA 1 cut(s) 1082
AsuNHI GCTAGC 1 cut(s) 652
AvaI CYCGRG 3 cut(s) 527, 864, 1947
AvaII GGWCC 3 cut(s) 1214, 1439, 2534
BalI TGGCCA 1 cut(s) 1236
BanII GRGCYC 1 cut(s) 372
BarI GAAGNNNNNNTAC 2 cut(s) 1008, 1040
BbrPI CACGTG 1 cut(s) 431
BbsI GAAGAC 3 cut(s) 30, 1180, 1538
Bbv12I GWGCWC 2 cut(s) 372, 534
BbvI GCAGC 1 cut(s) 783
BceAI ACGGC 3 cut(s) 286, 1012, 2650
BciT130I CCWGG 2 cut(s) 1449, 1521
BciVI GTATCC 2 cut(s) 708, 1742
BclI TGATCA 1 cut(s) 738
BcoDI GTCTC 5 cut(s) 419, 530, 602, 1268, 2911
BfaI CTAG 6 cut(s) 242, 399, 573, 653, 938, 1997
BfmI CTRYAG 2 cut(s) 720, 792
BfuAI ACCTGC 1 cut(s) 727
BfuI GTATCC 2 cut(s) 708, 1742
BglII AGATCT 4 cut(s) 12, 360, 538, 1228
BisI GCNGC 3 cut(s) 772, 2262, 2451
BlsI GCNGC 3 cut(s) 773, 2263, 2452
Bme1390I CCNGG 2 cut(s) 1449, 1521
Bme18I GGWCC 3 cut(s) 1214, 1439, 2534
BmeT110I CYCGRG 3 cut(s) 527, 864, 1947
BmgBI CACGTC 2 cut(s) 1842, 2338
BmgT120I GGNCC 3 cut(s) 1214, 1439, 2534
BmiI GGNNCC 2 cut(s) 1897, 2535
BmrFI CCNGG 2 cut(s) 1449, 1521
BmsI GCATC 1 cut(s) 2574
BmtI GCTAGC 1 cut(s) 656
BpiI GAAGAC 3 cut(s) 30, 1180, 1538
BplI GAGNNNNNCTC 2 cut(s) 1390, 1422
BpmI CTGGAG 4 cut(s) 443, 1377, 2424, 2631
Bpu10I CCTNAGC 1 cut(s) 2454
Bpu14I TTCGAA 1 cut(s) 1082
BpuEI CTTGAG 7 cut(s) 584, 613, 794, 875, 1028, 1352, 1535
Bsa29I ATCGAT 1 cut(s) 1116
BsaAI YACGTR 1 cut(s) 431
BsaBI GATNNNNATC 1 cut(s) 1112
BsaI GGTCTC 1 cut(s) 2911
BsaJI CCNNGG 7 cut(s) 631, 706, 1519, 1849, 1948, 2377, 2865
BsaWI WCCGGW 8 cut(s) 280, 341, 1067, 1436, 1500, 1652, 1663, 1796
Bsc4I CCNNNNNNNGG 6 cut(s) 1428, 1448, 1507, 1601, 1814, 1892
Bse118I RCCGGY 1 cut(s) 1500
Bse1I ACTGG 3 cut(s) 205, 1360, 2738
Bse3DI GCAATG 3 cut(s) 409, 2783, 2892
Bse8I GATNNNNATC 1 cut(s) 1112
BseAI TCCGGA 2 cut(s) 280, 1436
BseBI CCWGG 2 cut(s) 1449, 1521
BseCI ATCGAT 1 cut(s) 1116
BseDI CCNNGG 7 cut(s) 631, 706, 1519, 1849, 1948, 2377, 2865
BseJI GATNNNNATC 1 cut(s) 1112
BseLI CCNNNNNNNGG 6 cut(s) 1428, 1448, 1507, 1601, 1814, 1892
BseMI GCAATG 3 cut(s) 409, 2783, 2892
BseNI ACTGG 3 cut(s) 205, 1360, 2738
BseRI GAGGAG 1 cut(s) 39
BseXI GCAGC 1 cut(s) 783
Bsh1236I CGCG 1 cut(s) 708
Bsh1285I CGRYCG 1 cut(s) 2622
BshFI GGCC 4 cut(s) 378, 1236, 1992, 2864
BshTI ACCGGT 1 cut(s) 1500
BshVI ATCGAT 1 cut(s) 1116
BsiEI CGRYCG 1 cut(s) 2622
BsiHKAI GWGCWC 2 cut(s) 372, 534
BsiHKCI CYCGRG 3 cut(s) 527, 864, 1947
BsiSI CCGG 9 cut(s) 267, 281, 342, 1068, 1437, 1501, 1653, 1664, 1797
BsiWI CGTACG 1 cut(s) 2631
BslFI GGGAC 1 cut(s) 1524
BslI CCNNNNNNNGG 6 cut(s) 1428, 1448, 1507, 1601, 1814, 1892
BsmAI GTCTC 5 cut(s) 419, 530, 602, 1268, 2911
BsmBI CGTCTC 2 cut(s) 530, 1268
BsmFI GGGAC 1 cut(s) 1524
BsnI GGCC 4 cut(s) 378, 1236, 1992, 2864
Bso31I GGTCTC 1 cut(s) 2911
BsoBI CYCGRG 3 cut(s) 527, 864, 1947
Bsp119I TTCGAA 1 cut(s) 1082
Bsp1286I GDGCHC 2 cut(s) 372, 534
Bsp13I TCCGGA 2 cut(s) 280, 1436
Bsp1407I TGTACA 1 cut(s) 2691
Bsp19I CCATGG 1 cut(s) 2865
BspACI CCGC 8 cut(s) 706, 708, 1247, 1379, 1962, 2157, 2262, 2450
BspANI GGCC 4 cut(s) 378, 1236, 1992, 2864
BspDI ATCGAT 1 cut(s) 1116
BspEI TCCGGA 2 cut(s) 280, 1436
BspFNI CGCG 1 cut(s) 708
BspHI TCATGA 1 cut(s) 2245
BspLI GGNNCC 2 cut(s) 1897, 2535
BspMAI CTGCAG 2 cut(s) 724, 796
BspMI ACCTGC 1 cut(s) 727
BspOI GCTAGC 1 cut(s) 656
BspQI GCTCTTC 2 cut(s) 67, 2675
BspT104I TTCGAA 1 cut(s) 1082
BspTNI GGTCTC 1 cut(s) 2911
BsrDI GCAATG 3 cut(s) 409, 2783, 2892
BsrFI RCCGGY 1 cut(s) 1500
BsrGI TGTACA 1 cut(s) 2691
BsrI ACTGG 3 cut(s) 205, 1360, 2738
BssAI RCCGGY 1 cut(s) 1500
BssECI CCNNGG 7 cut(s) 631, 706, 1519, 1849, 1948, 2377, 2865
BssT1I CCWWGG 2 cut(s) 1849, 2865
Bst2UI CCWGG 2 cut(s) 1449, 1521
Bst4CI ACNGT 8 cut(s) 335, 563, 1895, 2255, 2430, 2504, 2801, 2874
Bst6I CTCTTC 7 cut(s) 67, 983, 1199, 1368, 1535, 1751, 2675
BstAUI TGTACA 1 cut(s) 2691
BstBAI YACGTR 1 cut(s) 431
BstBI TTCGAA 1 cut(s) 1082
BstC8I GCNNGC 5 cut(s) 654, 2316, 2486, 2571, 2626
BstDSI CCRYGG 3 cut(s) 631, 706, 2865
BstFNI CGCG 1 cut(s) 708
BstHHI GCGC 1 cut(s) 1390
BstMAI GTCTC 5 cut(s) 419, 530, 602, 1268, 2911
BstMCI CGRYCG 1 cut(s) 2622
BstMWI GCNNNNNNNGC 6 cut(s) 1333, 1385, 2447, 2555, 2634, 2896
BstNI CCWGG 2 cut(s) 1449, 1521
BstNSI RCATGY 2 cut(s) 1615, 2614
BstSCI CCNGG 2 cut(s) 1447, 1519
BstSFI CTRYAG 2 cut(s) 720, 792
BstUI CGCG 1 cut(s) 708
BstV1I GCAGC 1 cut(s) 783
BstV2I GAAGAC 3 cut(s) 30, 1180, 1538
BstX2I RGATCY 9 cut(s) 12, 360, 538, 567, 1002, 1228, 1515, 1943, 2787
BstXI CCANNNNNNTGG 1 cut(s) 2147
BstYI RGATCY 9 cut(s) 12, 360, 538, 567, 1002, 1228, 1515, 1943, 2787
Bsu15I ATCGAT 1 cut(s) 1116
BsuI GTATCC 2 cut(s) 708, 1742
BsuRI GGCC 4 cut(s) 378, 1236, 1992, 2864
BsuTUI ATCGAT 1 cut(s) 1116
BtgI CCRYGG 3 cut(s) 631, 706, 2865
BtgZI GCGATG 2 cut(s) 2626, 2700
BtrI CACGTC 2 cut(s) 1842, 2338
BtsIMutI CAGTG 1 cut(s) 2663
BveI ACCTGC 1 cut(s) 727
Cac8I GCNNGC 5 cut(s) 654, 2316, 2486, 2571, 2626
CaiI CAGNNNCTG 1 cut(s) 857
CciI TCATGA 1 cut(s) 2245
CfoI GCGC 1 cut(s) 1390
Cfr10I RCCGGY 1 cut(s) 1500
Cfr13I GGNCC 3 cut(s) 1214, 1439, 2534
Cfr42I CCGCGG 1 cut(s) 709
ClaI ATCGAT 1 cut(s) 1116
CpoI CGGWCCG 1 cut(s) 1439
CseI GACGC 1 cut(s) 476
Csp6I GTAC 4 cut(s) 674, 1805, 2632, 2692
CspAI ACCGGT 1 cut(s) 1500
CspI CGGWCCG 1 cut(s) 1439
CviQI GTAC 4 cut(s) 674, 1805, 2632, 2692
EaeI YGGCCR 1 cut(s) 1234
Eam1104I CTCTTC 7 cut(s) 67, 983, 1199, 1368, 1535, 1751, 2675
EarI CTCTTC 7 cut(s) 67, 983, 1199, 1368, 1535, 1751, 2675
EciI GGCGGA 1 cut(s) 2172
Ecl136II GAGCTC 1 cut(s) 370
Eco130I CCWWGG 2 cut(s) 1849, 2865
Eco147I AGGCCT 2 cut(s) 378, 1992
Eco24I GRGCYC 1 cut(s) 372
Eco31I GGTCTC 1 cut(s) 2911
Eco32I GATATC 1 cut(s) 1674
Eco47I GGWCC 3 cut(s) 1214, 1439, 2534
Eco53kI GAGCTC 1 cut(s) 370
Eco57I CTGAAG 5 cut(s) 818, 947, 1245, 1460, 1797
Eco72I CACGTG 1 cut(s) 431
Eco88I CYCGRG 3 cut(s) 527, 864, 1947
EcoICRI GAGCTC 1 cut(s) 370
EcoO109I RGGNCCY 1 cut(s) 2534
EcoRI GAATTC 1 cut(s) 1714
EcoRII CCWGG 2 cut(s) 1447, 1519
EcoRV GATATC 1 cut(s) 1674
EcoT14I CCWWGG 2 cut(s) 1849, 2865
EcoT22I ATGCAT 1 cut(s) 2659
EcoT38I GRGCYC 1 cut(s) 372
ErhI CCWWGG 2 cut(s) 1849, 2865
Esp3I CGTCTC 2 cut(s) 530, 1268
FalI AAGNNNNNCTT 4 cut(s) 1964, 1996, 2204, 2236
FaqI GGGAC 1 cut(s) 1524
FauI CCCGC 1 cut(s) 1372
FbaI TGATCA 1 cut(s) 738
FblI GTMKAC 2 cut(s) 1124, 2172
Fnu4HI GCNGC 3 cut(s) 772, 2262, 2451
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 3 cut(s) 772, 2262, 2451
FspBI CTAG 6 cut(s) 242, 399, 573, 653, 938, 1997
FspI TGCGCA 1 cut(s) 1389
GlaI GCGC 1 cut(s) 1389
GluI GCNGC 3 cut(s) 772, 2262, 2451
GsuI CTGGAG 4 cut(s) 443, 1377, 2424, 2631
HaeIII GGCC 4 cut(s) 378, 1236, 1992, 2864
HapII CCGG 9 cut(s) 267, 281, 342, 1068, 1437, 1501, 1653, 1664, 1797
HgaI GACGC 1 cut(s) 476
HhaI GCGC 1 cut(s) 1390
Hin6I GCGC 1 cut(s) 1388
HinP1I GCGC 1 cut(s) 1388
HindIII AAGCTT 3 cut(s) 959, 1046, 2075
HpaII CCGG 9 cut(s) 267, 281, 342, 1068, 1437, 1501, 1653, 1664, 1797
HphI GGTGA 8 cut(s) 66, 356, 575, 1057, 1616, 1964, 2465, 2830
Hpy166II GTNNAC 5 cut(s) 1125, 1244, 2173, 2353, 2692
Hpy8I GTNNAC 5 cut(s) 1125, 1244, 2173, 2353, 2692
Hpy99I CGWCG 2 cut(s) 563, 2021
HpyAV CCTTC 7 cut(s) 159, 407, 917, 1089, 1484, 1742, 2753
HpyCH4III ACNGT 8 cut(s) 335, 563, 1895, 2255, 2430, 2504, 2801, 2874
HpyCH4IV ACGT 3 cut(s) 430, 1841, 2337
HpyF10VI GCNNNNNNNGC 6 cut(s) 1333, 1385, 2447, 2555, 2634, 2896
HpySE526I ACGT 3 cut(s) 430, 1841, 2337
HspAI GCGC 1 cut(s) 1388
Kpn2I TCCGGA 2 cut(s) 280, 1436
Ksp22I TGATCA 1 cut(s) 738
KspI CCGCGG 1 cut(s) 709
LguI GCTCTTC 2 cut(s) 67, 2675
LmnI GCTCC 3 cut(s) 2597, 2650, 2716
Lsp1109I GCAGC 1 cut(s) 783
LweI GCATC 1 cut(s) 2574
MaeI CTAG 6 cut(s) 242, 399, 573, 653, 938, 1997
MaeII ACGT 3 cut(s) 430, 1841, 2337
MaeIII GTNAC 8 cut(s) 212, 303, 457, 689, 1155, 1614, 1637, 2801
MflI RGATCY 9 cut(s) 12, 360, 538, 567, 1002, 1228, 1515, 1943, 2787
MhlI GDGCHC 2 cut(s) 372, 534
MlsI TGGCCA 1 cut(s) 1236
MluNI TGGCCA 1 cut(s) 1236
MlyI GAGTC 8 cut(s) 442, 454, 514, 604, 889, 1163, 1552, 1645
MmeI TCCRAC 3 cut(s) 933, 1446, 2424
Mox20I TGGCCA 1 cut(s) 1236
Mph1103I ATGCAT 1 cut(s) 2659
MroI TCCGGA 2 cut(s) 280, 1436
MscI TGGCCA 1 cut(s) 1236
MseI TTAA 8 cut(s) 81, 389, 972, 1722, 1763, 1926, 2130, 2222
MslI CAYNNNNRTG 1 cut(s) 2505
Msp20I TGGCCA 1 cut(s) 1236
MspA1I CMGCKG 1 cut(s) 708
MspI CCGG 9 cut(s) 267, 281, 342, 1068, 1437, 1501, 1653, 1664, 1797
MspR9I CCNGG 2 cut(s) 1449, 1521
MvaI CCWGG 2 cut(s) 1449, 1521
MvnI CGCG 1 cut(s) 708
MwoI GCNNNNNNNGC 6 cut(s) 1333, 1385, 2447, 2555, 2634, 2896
NcoI CCATGG 1 cut(s) 2865
NheI GCTAGC 1 cut(s) 652
NlaIV GGNNCC 2 cut(s) 1897, 2535
NmuCI GTSAC 6 cut(s) 212, 303, 457, 1155, 1614, 1637
NsbI TGCGCA 1 cut(s) 1389
NsiI ATGCAT 1 cut(s) 2659
NspI RCATGY 2 cut(s) 1615, 2614
NspV TTCGAA 1 cut(s) 1082
PaeR7I CTCGAG 2 cut(s) 527, 864
PagI TCATGA 1 cut(s) 2245
PceI AGGCCT 2 cut(s) 378, 1992
PciSI GCTCTTC 2 cut(s) 67, 2675
PcsI WCGNNNNNNNCGW 1 cut(s) 2682
Pfl23II CGTACG 1 cut(s) 2631
PinAI ACCGGT 1 cut(s) 1500
PkrI GCNGC 3 cut(s) 773, 2263, 2452
Ple19I CGATCG 1 cut(s) 2622
PleI GAGTC 8 cut(s) 442, 454, 513, 603, 889, 1162, 1551, 1644
PmaCI CACGTG 1 cut(s) 431
PmlI CACGTG 1 cut(s) 431
PpsI GAGTC 8 cut(s) 442, 454, 513, 603, 889, 1162, 1551, 1644
Ppu21I YACGTR 1 cut(s) 431
PpuMI RGGWCCY 1 cut(s) 2534
PsiI TTATAA 1 cut(s) 2495
Psp124BI GAGCTC 1 cut(s) 372
Psp5II RGGWCCY 1 cut(s) 2534
Psp6I CCWGG 2 cut(s) 1447, 1519
PspCI CACGTG 1 cut(s) 431
PspGI CCWGG 2 cut(s) 1447, 1519
PspLI CGTACG 1 cut(s) 2631
PspN4I GGNNCC 2 cut(s) 1897, 2535
PspPI GGNCC 3 cut(s) 1214, 1439, 2534
PspPPI RGGWCCY 1 cut(s) 2534
PstI CTGCAG 2 cut(s) 724, 796
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 9 cut(s) 12, 360, 538, 567, 1002, 1228, 1515, 1943, 2787
PvuI CGATCG 1 cut(s) 2622
RsaI GTAC 4 cut(s) 675, 1806, 2633, 2693
RsaNI GTAC 4 cut(s) 674, 1805, 2632, 2692
RseI CAYNNNNRTG 1 cut(s) 2505
Rsr2I CGGWCCG 1 cut(s) 1439
RsrII CGGWCCG 1 cut(s) 1439
SacI GAGCTC 1 cut(s) 372
SacII CCGCGG 1 cut(s) 709
SapI GCTCTTC 2 cut(s) 67, 2675
SaqAI TTAA 8 cut(s) 81, 389, 972, 1722, 1763, 1926, 2130, 2222
SatI GCNGC 3 cut(s) 772, 2262, 2451
Sau96I GGNCC 3 cut(s) 1214, 1439, 2534
SchI GAGTC 8 cut(s) 442, 454, 514, 604, 889, 1163, 1552, 1645
ScrFI CCNGG 2 cut(s) 1449, 1521
SduI GDGCHC 2 cut(s) 372, 534
SfaNI GCATC 1 cut(s) 2574
SfcI CTRYAG 2 cut(s) 720, 792
Sfr274I CTCGAG 2 cut(s) 527, 864
Sfr303I CCGCGG 1 cut(s) 709
SfuI TTCGAA 1 cut(s) 1082
SgrBI CCGCGG 1 cut(s) 709
SinI GGWCC 3 cut(s) 1214, 1439, 2534
SlaI CTCGAG 2 cut(s) 527, 864
SmiMI CAYNNNNRTG 1 cut(s) 2505
SmlI CTYRAG 9 cut(s) 527, 592, 599, 809, 864, 890, 1043, 1331, 1550
SmoI CTYRAG 9 cut(s) 527, 592, 599, 809, 864, 890, 1043, 1331, 1550
SseBI AGGCCT 2 cut(s) 378, 1992
SsiI CCGC 8 cut(s) 706, 708, 1247, 1379, 1962, 2157, 2262, 2450
SspI AATATT 1 cut(s) 2848
SspMI CTAG 6 cut(s) 242, 399, 573, 653, 938, 1997
SstI GAGCTC 1 cut(s) 372
StuI AGGCCT 2 cut(s) 378, 1992
StyD4I CCNGG 2 cut(s) 1447, 1519
StyI CCWWGG 2 cut(s) 1849, 2865
TaaI ACNGT 8 cut(s) 335, 563, 1895, 2255, 2430, 2504, 2801, 2874
TaiI ACGT 3 cut(s) 433, 1844, 2340
TaqII GACCGA 3 cut(s) 1202, 1456, 2935
TatI WGTACW 1 cut(s) 2691
TauI GCSGC 2 cut(s) 2264, 2453
Tru1I TTAA 8 cut(s) 81, 389, 972, 1722, 1763, 1926, 2130, 2222
Tru9I TTAA 8 cut(s) 81, 389, 972, 1722, 1763, 1926, 2130, 2222
TscAI CASTG 1 cut(s) 2670
TseFI GTSAC 6 cut(s) 212, 303, 457, 1155, 1614, 1637
TseI GCWGC 1 cut(s) 771
Tsp45I GTSAC 6 cut(s) 212, 303, 457, 1155, 1614, 1637
TspDTI ATGAA 3 cut(s) 150, 740, 1769
TspGWI ACGGA 4 cut(s) 504, 620, 1250, 2208
TspRI CASTG 1 cut(s) 2670
VpaK11BI GGWCC 3 cut(s) 1214, 1439, 2534
XapI RAATTY 4 cut(s) 175, 1714, 1967, 2065
XbaI TCTAGA 2 cut(s) 241, 937
XceI RCATGY 2 cut(s) 1615, 2614
XhoI CTCGAG 2 cut(s) 527, 864
XmiI GTMKAC 2 cut(s) 1124, 2172
XspI CTAG 6 cut(s) 242, 399, 573, 653, 938, 1997
Zsp2I ATGCAT 1 cut(s) 2659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.