MD05G1241900.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012854
Physical Location & Seq
Forward (+)
37501499 .. 37506476
4978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1241900.v1.1.491

Sequence Viewer

Length: 2964 bp
ATGGCGAATTCAGGAATGAAGTCTTTTTTCCTTTGCTCTCTCTTTGTGTTCCTAACATGTTTTTCTTCTGTTTCTTCACATAATCTGTCTCTCAGGAGGCAAGCTTCGATCCTTGTTTCTCTCAAACAAAATTTCGAAGACTCGAAAAATCCTTCTTTAAGTACTTGGAATGTGTCAAACTACATGTTCCTCTGCTCTTGGGCTGGAATCCGTTGTGACGGCCTAAACAGATCAGTTGTCTCACTTGACATATCCAACTACAACCTCTCAGGTACTCTTTCGCCAGCGATCACTGAACTCCGAACCCTAGTCAATGTTTCGGTGTCAGGAAATGGATTTTCGGGTACTTTTCCTTCCGGTGTTCACAAGCTAGCCAGGCTACAAAACCTCAATATATCCAACAATGGGTTCAGCGGAAGCTTGGACTGGGAGTTTTCTCAGTTGAAGGAGCTTGTATTGCTAGATGCTTACAACAACGATTTCAATGGCTCCCTGCCATTAGGTGTCACTCAACTTCCGAAGCTGAAGCGGTTGGACTTTGGCGGGAACTACTTCAGTGGGAGCATTCCTCCAAGCTATGGAAACATGGTGCAGCTCAATTATCTCTCAGTTGCGGGAAATGACCTGAGCGGTTACATACCGCGCGAGCTCGGAAACCTCACTAACCTACGGCAGCTTTTGTTGGGATATTACAACGAATTTGAAGGTGGAATCCCACCGGAAATTGGCAAGCTGATCAATCTGTTTCATTTAGACCTCGCAAACTGTGGTTTGGAGGGGCCAATCCCTCTGGAGCTAGGCAATCTGAAAAAGCTAGACACTTTGTTCTTGCAAACAAATCAGCTCAGCGGTTCGATTCCTGCTCAGCTGGGAAACTTGAGTAGCTTGAGGTCTCTTGATCTGTCAAACAATGCGCTGACGGGAGATATCCCCGCTGAGTTCTCTGGACTCCGTGAGCTCACACTCCTGAACCTCTTTATCAACAAGTTTCACGGGGAGATTCCTCGCTCTATCGCGGAGCTACCAAAATTGGAAGTCTTGAAGCTGTGGCATAACAACTTCACTGGAGCCATACCTTCAAAGCTCGGTCAGAATGGTAAACTGATTGAGCTTGATCTTTCGAGTAACAAGCTCACCGGGGTTGTCCCAAAATCTCTTTGCTTCGGGAGGCGGCTGAAGATCTTGATTCTGCTCAACAATTTTCTCTTTGGGCCTCTACCTGATGATCTTGGCAAATGTGACACGCTAGTAAGAGTCCGAATGGGGCAGAATTATCTGACTGGATCAATACCACGGGGGTTTCTTTACTTGCCAGAGCTCTCACTAGTAGAATTGCAGAACAACTATCTCACTGGACAGCTTCCGCAGGAAACAAGAAAGCAACCCTCAAAACTCAGCCAGCTGAATCTGTCAAGCAATCGCTTATCCGGGTGCCTCCCTGCTTCTATCGGAAACTTTTCAGGCCTGCAGATTCTTCTATTGAGTGGAAACCAATTCACTGGAGAAATCCCATCTGACGTCGGCCGGTTGCAAAGCGTTCTCAAGTTGGATGTGAGCAGAAACAATTTTTCGGGCACAATACCTCCGGAAATTGGAAACTGTCTCTCTTTAACCTACTTAGATTTCAGCCAAAACCAACTCTCAGGTCCAATCCCAGTTCAGATTGTTCAAATCCACATACTAAATTACTTCAACGTTTCGTGGAACCACTTAAACCAGAGCCTCCCAAAGGAGCTAGGCTCCTTGAAAAGCCTTACGTCTGCTGACTTTTCGCACAACGACTTCTCCGGTTCAATCCCACAAACAGGACAATACTTGTTCTTCAACTCCACATCCTTTGTCGGTAACCCTGAGCTCTGCGATTCTTCTGCGAATCCATGCAAATACTCCTCAACCTCAGCATCAGAAGATCACAGCCAAACCGGCACGCACTCTCAAGTCCTCGGCAAATTCAAGCTTGTCTTTGCACTAGGCCTCTTGCTGTGCTCGTTCGTGTTTGCAACCGTTGCAATTATCAAGACCAGAAAGGTGCGAAAACATTCGAACTCGTGGAAGCTCACAGCATTCCAAAAGCTGGAATTTGGAAGTGAAGACATCTTAGAGTGCATAAAGGATAACAATGTGATAGGGAGAGGTGGAGCTGGGATTGTCTACAGAGGAACAATGTTGAGCGGCGAGCAAGTGGCGGTGAAGAAGCTGTTGGGAATCAACAAAGGCTCGTCACACGACAACGGCCTCTCCGCAGAAATTCAAACACTGGGAAAAATCCGCCACCGGAACATTGTCCGGTTGCTGGCATTCTGTTCAAACAAAGAGACCAATCTGCTGGTTTACGAGTACATGCCGAATGGAAGTTTAGGTGAAGTTTTGCATGGGAAGGGAGGAGGATATCTCAAGTGGGAAACTAGGTTGAATATTGCCATTGAAGCAGCGAAAGGGCTCTGCTATTTGCACCATGATTGTTCTCCTCTGATTCTTCATAGGGATGTTAAGTCCAACAACATTCTGCTCAACTCGGAATTTGAGGCTCATGTTGCAGATTTTGGGCTTGCCAAGTTTTTGCAGGACACTGGAACATCAGAATGCATGTCTGCAATTGCTGGCTCATACGGTTACATTGCTCCAGAGTACGCATACACATTGAGAGTTGACGAGAAGAGCGATGTGTATAGCTTTGGAGTTGTACTGTTGGAGCTCATCACAGGAAGAAGGCCAGTTGGTGGATTTGGGGAAGAGGGAATGGACATTGTTCAGTGGACCAAGATTCAGACCAACTCGCAGAAAGAAGGGGTTATAAAGATCCTCGACAAACGGCTAGACACTGTTCCGATGGACGAAGCAATGCAGGTATTTTTCGTCGCGGTTTTATGTGTTCAAGAGCAAAGTGTGGAGAGACCAACCATGAGAGAAGTTGTGCAAATGCTTGCACAGGCTAAACAACCAAACACATTTTACATGCAATGA

Protein Analysis

988

Amino Acids

108.54

Weight (kDa)

7.77

Isoelectric Point (pI)

33.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 73 2.6e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 121 - 228 1.7e-08 Leucine-rich repeat region
LRR_14 PF23598 238 - 327 3.5e-07 Leucine-rich repeat region
LRR_8 PF13855 271 - 329 9.8e-08 Leucine rich repeat
LRR_8 PF13855 342 - 400 2.9e-06 Leucine rich repeat
LRR_8 PF13855 438 - 499 2.2e-06 Leucine rich repeat
LRR_14 PF23598 467 - 582 2e-06 Leucine-rich repeat region
Pkinase PF00069 705 - 973 3.3e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 706 - 975 4.1e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012854)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20270
fragaria_vesca FvH4_3g13410
malus_domestica MD05G1241900.v1.1 MD10G1223800.v1.1
prunus_persica Prupe.4G121500_v2.0.a1
pyrus_communis pycom05g21850 pycom10g18770 pycom10g18780
rosa_chinensis RchiOBHm_Chr5g0022141
rosa_laevigata RLG00000032647
rosa_multiflora Rmu_sc0000491.1_g000007
rosa_roxburghii Rroxscaffold_1G00056790
rosa_rugosa Rorug05G0071500.1
rosa_samantha Rh5AG159600 Rh5BG159100 Rh5CG173300 Rh5DG159100
rosa_wichuraiana Rw5G014250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2795
AatII GACGTC 1 cut(s) 1521
Acc36I ACCTGC 1 cut(s) 2836
AccB1I GGYRCC 1 cut(s) 1431
AccB7I CCANNNNNTGG 3 cut(s) 578, 2074, 2720
AccBSI CCGCTC 2 cut(s) 630, 2172
AccI GTMKAC 1 cut(s) 2151
AccII CGCG 4 cut(s) 643, 645, 1016, 2861
AccIII TCCGGA 1 cut(s) 1585
AclI AACGTT 1 cut(s) 1695
AclWI GGATC 3 cut(s) 103, 1291, 2794
AcoI YGGCCR 1 cut(s) 1522
AcsI RAATTY 7 cut(s) 7, 130, 698, 1949, 2078, 2247, 2519
AcuI CTGAAG 3 cut(s) 538, 545, 1196
AcyI GRCGYC 1 cut(s) 1518
AfaI GTAC 6 cut(s) 163, 274, 346, 2339, 2630, 2685
AfiI CCNNNNNNNGG 9 cut(s) 405, 578, 725, 1592, 1729, 1805, 2074, 2293, 2720
AflIII ACRYGT 2 cut(s) 56, 183
AhlI ACTAGT 1 cut(s) 1324
AjnI CCWGG 1 cut(s) 374
AjuI GAANNNNNNNTTGG 4 cut(s) 392, 424, 2183, 2215
Alw21I GWGCWC 6 cut(s) 651, 960, 1320, 1857, 1988, 2697
Alw26I GTCTC 6 cut(s) 93, 244, 897, 1607, 2309, 2887
AlwI GGATC 3 cut(s) 103, 1291, 2794
Aor13HI TCCGGA 1 cut(s) 1585
AoxI GGCC 8 cut(s) 220, 779, 1211, 1462, 1522, 1972, 2233, 2711
ApeKI GCWGC 3 cut(s) 592, 673, 2429
ApoI RAATTY 7 cut(s) 7, 130, 698, 1949, 2078, 2247, 2519
Asp700I GAANNNNTTC 3 cut(s) 551, 1456, 2038
AspLEI GCGC 2 cut(s) 645, 916
AspS9I GGNCC 4 cut(s) 779, 1211, 1646, 2757
AsuC2I CCSGG 2 cut(s) 1138, 1429
AsuHPI GGTGA 3 cut(s) 1126, 2200, 2372
AsuII TTCGAA 2 cut(s) 135, 2042
AsuNHI GCTAGC 1 cut(s) 370
AvaII GGWCC 2 cut(s) 1646, 2757
BaeGI GKGCMC 1 cut(s) 1577
BanI GGYRCC 1 cut(s) 1431
BanII GRGCYC 6 cut(s) 651, 960, 1320, 1857, 2442, 2697
BarI GAAGNNNNNNTAC 2 cut(s) 337, 369
BauI CACGAG 1 cut(s) 2047
BbsI GAAGAC 2 cut(s) 144, 2097
Bbv12I GWGCWC 6 cut(s) 651, 960, 1320, 1857, 1988, 2697
BbvCI CCTCAGC 1 cut(s) 1897
BbvI GCAGC 3 cut(s) 604, 685, 2441
BccI CCATC 2 cut(s) 1519, 2824
BceAI ACGGC 4 cut(s) 235, 686, 2248, 2828
BcgI CGANNNNNNTGC 2 cut(s) 1850, 1884
BciT130I CCWGG 1 cut(s) 376
BclI TGATCA 1 cut(s) 735
BcnI CCSGG 2 cut(s) 1138, 1429
BcoDI GTCTC 6 cut(s) 93, 244, 897, 1607, 2309, 2887
BcuI ACTAGT 1 cut(s) 1324
BfmI CTRYAG 2 cut(s) 1466, 2152
BfuAI ACCTGC 1 cut(s) 2836
BglI GCCNNNNNGGC 1 cut(s) 1923
BglII AGATCT 1 cut(s) 1179
BisI GCNGC 5 cut(s) 593, 674, 1172, 2173, 2430
BlpI GCTNAGC 2 cut(s) 845, 864
BlsI GCNGC 5 cut(s) 594, 675, 1173, 2174, 2431
BmcAI AGTACT 1 cut(s) 163
Bme1390I CCNGG 3 cut(s) 376, 1138, 1429
Bme18I GGWCC 2 cut(s) 1646, 2757
BmgT120I GGNCC 4 cut(s) 779, 1211, 1646, 2757
BmiI GGNNCC 6 cut(s) 490, 780, 1069, 1433, 1706, 1741
BmrFI CCNGG 3 cut(s) 376, 1138, 1429
BmrI ACTGGG 3 cut(s) 436, 1649, 2267
BmsI GCATC 2 cut(s) 454, 1910
BmtI GCTAGC 1 cut(s) 374
BmuI ACTGGG 3 cut(s) 436, 1649, 2267
BpiI GAAGAC 2 cut(s) 144, 2097
BplI GAGNNNNNCTC 6 cut(s) 553, 585, 1724, 1756, 2376, 2408
BpmI CTGGAG 4 cut(s) 812, 1086, 1521, 2607
Bpu10I CCTNAGC 3 cut(s) 626, 1851, 1897
Bpu1102I GCTNAGC 2 cut(s) 845, 864
Bpu14I TTCGAA 2 cut(s) 135, 2042
BpuEI CTTGAG 5 cut(s) 898, 907, 1526, 1920, 2378
BpuMI CCSGG 2 cut(s) 1138, 1429
BsaHI GRCGYC 1 cut(s) 1518
BsaI GGTCTC 3 cut(s) 897, 2309, 2887
BsaJI CCNNGG 3 cut(s) 1137, 1292, 1942
BsaWI WCCGGW 6 cut(s) 356, 718, 1585, 1787, 2274, 2286
BsaXI ACNNNNNCTCC 6 cut(s) 1567, 1597, 1769, 1799, 2222, 2252
Bsc4I CCNNNNNNNGG 9 cut(s) 405, 578, 725, 1592, 1729, 1805, 2074, 2293, 2720
Bse118I RCCGGY 2 cut(s) 1524, 1922
Bse1I ACTGG 9 cut(s) 431, 1069, 1285, 1357, 1504, 1655, 2262, 2575, 2714
Bse3DI GCAATG 2 cut(s) 2616, 2847
BseAI TCCGGA 1 cut(s) 1585
BseBI CCWGG 1 cut(s) 376
BseDI CCNNGG 3 cut(s) 1137, 1292, 1942
BseGI GGATG 3 cut(s) 1555, 1832, 2491
BseLI CCNNNNNNNGG 9 cut(s) 405, 578, 725, 1592, 1729, 1805, 2074, 2293, 2720
BseMI GCAATG 2 cut(s) 2616, 2847
BseNI ACTGG 9 cut(s) 431, 1069, 1285, 1357, 1504, 1655, 2262, 2575, 2714
BseRI GAGGAG 3 cut(s) 1879, 2397, 2457
BseSI GKGCMC 1 cut(s) 1577
BseX3I CGGCCG 1 cut(s) 1522
BseXI GCAGC 3 cut(s) 604, 685, 2441
BseYI CCCAGC 2 cut(s) 868, 2141
BsgI GTGCAG 1 cut(s) 611
Bsh1236I CGCG 4 cut(s) 643, 645, 1016, 2861
Bsh1285I CGRYCG 1 cut(s) 1525
BshFI GGCC 8 cut(s) 222, 781, 1213, 1464, 1524, 1974, 2235, 2713
BshNI GGYRCC 1 cut(s) 1431
BsiEI CGRYCG 1 cut(s) 1525
BsiHKAI GWGCWC 6 cut(s) 651, 960, 1320, 1857, 1988, 2697
BslFI GGGAC 1 cut(s) 1130
BslI CCNNNNNNNGG 9 cut(s) 405, 578, 725, 1592, 1729, 1805, 2074, 2293, 2720
BsmAI GTCTC 6 cut(s) 93, 244, 897, 1607, 2309, 2887
BsmFI GGGAC 1 cut(s) 1130
BsmI GAATGC 4 cut(s) 564, 2063, 2297, 2588
BsnI GGCC 8 cut(s) 222, 781, 1213, 1464, 1524, 1974, 2235, 2713
Bso31I GGTCTC 3 cut(s) 897, 2309, 2887
Bsp119I TTCGAA 2 cut(s) 135, 2042
Bsp1286I GDGCHC 8 cut(s) 651, 960, 1320, 1577, 1857, 1988, 2442, 2697
Bsp13I TCCGGA 1 cut(s) 1585
Bsp1720I GCTNAGC 2 cut(s) 845, 864
BspANI GGCC 8 cut(s) 222, 781, 1213, 1464, 1524, 1974, 2235, 2713
BspEI TCCGGA 1 cut(s) 1585
BspFNI CGCG 4 cut(s) 643, 645, 1016, 2861
BspLI GGNNCC 6 cut(s) 490, 780, 1069, 1433, 1706, 1741
BspMAI CTGCAG 1 cut(s) 1470
BspMI ACCTGC 1 cut(s) 2836
BspOI GCTAGC 1 cut(s) 374
BspPI GGATC 3 cut(s) 103, 1291, 2794
BspQI GCTCTTC 1 cut(s) 2651
BspT104I TTCGAA 2 cut(s) 135, 2042
BspT107I GGYRCC 1 cut(s) 1431
BspTNI GGTCTC 3 cut(s) 897, 2309, 2887
BsrBI CCGCTC 2 cut(s) 630, 2172
BsrDI GCAATG 2 cut(s) 2616, 2847
BsrFI RCCGGY 2 cut(s) 1524, 1922
BsrI ACTGG 9 cut(s) 431, 1069, 1285, 1357, 1504, 1655, 2262, 2575, 2714
BssAI RCCGGY 2 cut(s) 1524, 1922
BssECI CCNNGG 3 cut(s) 1137, 1292, 1942
BssNI GRCGYC 1 cut(s) 1518
BssSI CACGAG 1 cut(s) 2047
Bst2BI CACGAG 1 cut(s) 2047
Bst2UI CCWGG 1 cut(s) 376
Bst4CI ACNGT 6 cut(s) 767, 1601, 2005, 2612, 2688, 2824
Bst6I CTCTTC 2 cut(s) 2651, 2727
BstACI GRCGYC 1 cut(s) 1518
BstAPI GCANNNNNTGC 1 cut(s) 2006
BstBI TTCGAA 2 cut(s) 135, 2042
BstDSI CCRYGG 1 cut(s) 1292
BstEII GGTNACC 1 cut(s) 1844
BstENI CCTNNNNNAGG 1 cut(s) 1727
BstF5I GGATG 3 cut(s) 1555, 1832, 2491
BstFNI CGCG 4 cut(s) 643, 645, 1016, 2861
BstHHI GCGC 2 cut(s) 645, 916
BstMAI GTCTC 6 cut(s) 93, 244, 897, 1607, 2309, 2887
BstMCI CGRYCG 1 cut(s) 1525
BstMWI GCNNNNNNNGC 6 cut(s) 376, 457, 1923, 2006, 2426, 2534
BstNI CCWGG 1 cut(s) 376
BstNSI RCATGY 5 cut(s) 60, 187, 2344, 2590, 2959
BstPI GGTNACC 1 cut(s) 1844
BstSCI CCNGG 3 cut(s) 374, 1136, 1427
BstSFI CTRYAG 2 cut(s) 1466, 2152
BstSLI GKGCMC 1 cut(s) 1577
BstUI CGCG 4 cut(s) 643, 645, 1016, 2861
BstV1I GCAGC 3 cut(s) 604, 685, 2441
BstV2I GAAGAC 2 cut(s) 144, 2097
BstX2I RGATCY 2 cut(s) 1179, 2799
BstXI CCANNNNNNTGG 2 cut(s) 1499, 2326
BstYI RGATCY 2 cut(s) 1179, 2799
BstZI CGGCCG 1 cut(s) 1522
BsuRI GGCC 8 cut(s) 222, 781, 1213, 1464, 1524, 1974, 2235, 2713
BtgI CCRYGG 1 cut(s) 1292
BtgZI GCGATG 1 cut(s) 2676
BtsCI GGATG 3 cut(s) 1555, 1832, 2491
BtsIMutI CAGTG 9 cut(s) 291, 562, 1062, 1350, 1497, 2255, 2568, 2759, 2820
BveI ACCTGC 1 cut(s) 2836
CfoI GCGC 2 cut(s) 645, 916
Cfr10I RCCGGY 2 cut(s) 1524, 1922
Cfr13I GGNCC 4 cut(s) 779, 1211, 1646, 2757
Csp6I GTAC 6 cut(s) 162, 273, 345, 2338, 2629, 2684
CspCI CAANNNNNGTGG 2 cut(s) 1819, 1854
CviQI GTAC 6 cut(s) 162, 273, 345, 2338, 2629, 2684
EaeI YGGCCR 1 cut(s) 1522
EagI CGGCCG 1 cut(s) 1522
Eam1104I CTCTTC 2 cut(s) 2651, 2727
EarI CTCTTC 2 cut(s) 2651, 2727
EciI GGCGGA 1 cut(s) 2258
Ecl136II GAGCTC 5 cut(s) 649, 958, 1318, 1855, 2695
EclXI CGGCCG 1 cut(s) 1522
Eco147I AGGCCT 2 cut(s) 1464, 1974
Eco24I GRGCYC 6 cut(s) 651, 960, 1320, 1857, 2442, 2697
Eco31I GGTCTC 3 cut(s) 897, 2309, 2887
Eco32I GATATC 2 cut(s) 928, 2390
Eco47I GGWCC 2 cut(s) 1646, 2757
Eco52I CGGCCG 1 cut(s) 1522
Eco53kI GAGCTC 5 cut(s) 649, 958, 1318, 1855, 2695
Eco57I CTGAAG 3 cut(s) 538, 545, 1196
Eco91I GGTNACC 1 cut(s) 1844
EcoICRI GAGCTC 5 cut(s) 649, 958, 1318, 1855, 2695
EcoNI CCTNNNNNAGG 1 cut(s) 1727
EcoO65I GGTNACC 1 cut(s) 1844
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 374
EcoRV GATATC 2 cut(s) 928, 2390
EcoT22I ATGCAT 1 cut(s) 2588
EcoT38I GRGCYC 6 cut(s) 651, 960, 1320, 1857, 2442, 2697
FalI AAGNNNNNCTT 2 cut(s) 1946, 1978
FaqI GGGAC 1 cut(s) 1130
FauI CCCGC 3 cut(s) 536, 607, 940
FbaI TGATCA 1 cut(s) 735
FblI GTMKAC 1 cut(s) 2151
Fnu4HI GCNGC 5 cut(s) 593, 674, 1172, 2173, 2430
FokI GGATG 3 cut(s) 1562, 1819, 2498
FriOI GRGCYC 6 cut(s) 651, 960, 1320, 1857, 2442, 2697
Fsp4HI GCNGC 5 cut(s) 593, 674, 1172, 2173, 2430
GlaI GCGC 2 cut(s) 644, 915
GluI GCNGC 5 cut(s) 593, 674, 1172, 2173, 2430
GsaI CCCAGC 2 cut(s) 872, 2145
GsuI CTGGAG 4 cut(s) 812, 1086, 1521, 2607
HaeIII GGCC 8 cut(s) 222, 781, 1213, 1464, 1524, 1974, 2235, 2713
HhaI GCGC 2 cut(s) 645, 916
Hin1I GRCGYC 1 cut(s) 1518
Hin6I GCGC 2 cut(s) 643, 914
HinP1I GCGC 2 cut(s) 643, 914
HincII GTYRAC 1 cut(s) 2650
HindII GTYRAC 1 cut(s) 2650
HindIII AAGCTT 3 cut(s) 102, 418, 1955
HphI GGTGA 3 cut(s) 1126, 2200, 2372
Hpy166II GTNNAC 6 cut(s) 364, 1100, 2152, 2332, 2650, 2757
Hpy8I GTNNAC 6 cut(s) 364, 1100, 2152, 2332, 2650, 2757
Hpy99I CGWCG 2 cut(s) 1523, 2861
HpyAV CCTTC 8 cut(s) 162, 363, 439, 698, 1086, 2371, 2703, 2780
HpyCH4III ACNGT 6 cut(s) 767, 1601, 2005, 2612, 2688, 2824
HpyCH4IV ACGT 3 cut(s) 1518, 1695, 1757
HpyF10VI GCNNNNNNNGC 6 cut(s) 376, 457, 1923, 2006, 2426, 2534
HpySE526I ACGT 3 cut(s) 1518, 1695, 1757
Hsp92I GRCGYC 1 cut(s) 1518
HspAI GCGC 2 cut(s) 643, 914
Kpn2I TCCGGA 1 cut(s) 1585
Ksp22I TGATCA 1 cut(s) 735
LguI GCTCTTC 1 cut(s) 2651
Lsp1109I GCAGC 3 cut(s) 604, 685, 2441
LweI GCATC 2 cut(s) 454, 1910
MaeII ACGT 3 cut(s) 1518, 1695, 1757
MaeIII GTNAC 8 cut(s) 215, 505, 632, 1124, 1238, 1844, 2220, 2612
MbiI CCGCTC 2 cut(s) 630, 2172
MfeI CAATTG 1 cut(s) 2595
MflI RGATCY 2 cut(s) 1179, 2799
MhlI GDGCHC 8 cut(s) 651, 960, 1320, 1577, 1857, 1988, 2442, 2697
MlyI GAGTC 3 cut(s) 134, 942, 1263
MmeI TCCRAC 6 cut(s) 279, 423, 513, 1527, 2520, 2670
Mph1103I ATGCAT 1 cut(s) 2588
MroI TCCGGA 1 cut(s) 1585
MroXI GAANNNNTTC 3 cut(s) 551, 1456, 2038
MseI TTAA 4 cut(s) 158, 1610, 1712, 2490
MslI CAYNNNNRTG 2 cut(s) 2484, 2581
MspA1I CMGCKG 5 cut(s) 414, 849, 868, 935, 1402
MspR9I CCNGG 3 cut(s) 376, 1138, 1429
MunI CAATTG 1 cut(s) 2595
Mva1269I GAATGC 4 cut(s) 564, 2063, 2297, 2588
MvaI CCWGG 1 cut(s) 376
MvnI CGCG 4 cut(s) 643, 645, 1016, 2861
MwoI GCNNNNNNNGC 6 cut(s) 376, 457, 1923, 2006, 2426, 2534
NciI CCSGG 2 cut(s) 1138, 1429
NheI GCTAGC 1 cut(s) 370
NlaIV GGNNCC 6 cut(s) 490, 780, 1069, 1433, 1706, 1741
NmeAIII GCCGAG 1 cut(s) 1923
NmuCI GTSAC 4 cut(s) 215, 505, 1238, 2220
NsiI ATGCAT 1 cut(s) 2588
NspI RCATGY 5 cut(s) 60, 187, 2344, 2590, 2959
NspV TTCGAA 2 cut(s) 135, 2042
PceI AGGCCT 2 cut(s) 1464, 1974
PciI ACATGT 2 cut(s) 56, 183
PciSI GCTCTTC 1 cut(s) 2651
PcsI WCGNNNNNNNCGW 1 cut(s) 2658
PctI GAATGC 4 cut(s) 564, 2063, 2297, 2588
PdmI GAANNNNTTC 3 cut(s) 551, 1456, 2038
PflMI CCANNNNNTGG 3 cut(s) 578, 2074, 2720
PkrI GCNGC 5 cut(s) 594, 675, 1173, 2174, 2431
PleI GAGTC 3 cut(s) 134, 942, 1262
PpsI GAGTC 3 cut(s) 134, 942, 1262
PscI ACATGT 2 cut(s) 56, 183
PsiI TTATAA 1 cut(s) 2795
Psp124BI GAGCTC 5 cut(s) 651, 960, 1320, 1857, 2697
Psp1406I AACGTT 1 cut(s) 1695
Psp6I CCWGG 1 cut(s) 374
PspEI GGTNACC 1 cut(s) 1844
PspFI CCCAGC 2 cut(s) 868, 2141
PspGI CCWGG 1 cut(s) 374
PspN4I GGNNCC 6 cut(s) 490, 780, 1069, 1433, 1706, 1741
PspPI GGNCC 4 cut(s) 779, 1211, 1646, 2757
PstI CTGCAG 1 cut(s) 1470
PsuI RGATCY 2 cut(s) 1179, 2799
PvuII CAGCTG 2 cut(s) 868, 1402
RsaI GTAC 6 cut(s) 163, 274, 346, 2339, 2630, 2685
RsaNI GTAC 6 cut(s) 162, 273, 345, 2338, 2629, 2684
RseI CAYNNNNRTG 2 cut(s) 2484, 2581
SacI GAGCTC 5 cut(s) 651, 960, 1320, 1857, 2697
SapI GCTCTTC 1 cut(s) 2651
SaqAI TTAA 4 cut(s) 158, 1610, 1712, 2490
SatI GCNGC 5 cut(s) 593, 674, 1172, 2173, 2430
Sau96I GGNCC 4 cut(s) 779, 1211, 1646, 2757
ScaI AGTACT 1 cut(s) 163
SchI GAGTC 3 cut(s) 134, 942, 1263
ScrFI CCNGG 3 cut(s) 376, 1138, 1429
SduI GDGCHC 8 cut(s) 651, 960, 1320, 1577, 1857, 1988, 2442, 2697
SfaNI GCATC 2 cut(s) 454, 1910
SfcI CTRYAG 2 cut(s) 1466, 2152
SfuI TTCGAA 2 cut(s) 135, 2042
SinI GGWCC 2 cut(s) 1646, 2757
SmiMI CAYNNNNRTG 2 cut(s) 2484, 2581
SmlI CTYRAG 5 cut(s) 877, 886, 1541, 1935, 2393
SmoI CTYRAG 5 cut(s) 877, 886, 1541, 1935, 2393
SpeI ACTAGT 1 cut(s) 1324
SseBI AGGCCT 2 cut(s) 1464, 1974
SspI AATATT 1 cut(s) 2416
SstI GAGCTC 5 cut(s) 651, 960, 1320, 1857, 2697
StuI AGGCCT 2 cut(s) 1464, 1974
StyD4I CCNGG 3 cut(s) 374, 1136, 1427
TaaI ACNGT 6 cut(s) 767, 1601, 2005, 2612, 2688, 2824
TaiI ACGT 3 cut(s) 1521, 1698, 1760
TaqI TCGA 7 cut(s) 107, 135, 143, 854, 1121, 2042, 2805
TaqII GACCGA 1 cut(s) 1076
TatI WGTACW 3 cut(s) 161, 2337, 2683
TauI GCSGC 2 cut(s) 1174, 2175
Tru1I TTAA 4 cut(s) 158, 1610, 1712, 2490
Tru9I TTAA 4 cut(s) 158, 1610, 1712, 2490
TscAI CASTG 9 cut(s) 298, 562, 1069, 1357, 1504, 2262, 2575, 2759, 2827
TseFI GTSAC 4 cut(s) 215, 505, 1238, 2220
TseI GCWGC 3 cut(s) 592, 673, 2429
Tsp45I GTSAC 4 cut(s) 215, 505, 1238, 2220
TspDTI ATGAA 3 cut(s) 32, 737, 2468
TspGWI ACGGA 2 cut(s) 200, 941
TspRI CASTG 9 cut(s) 298, 562, 1069, 1357, 1504, 2262, 2575, 2759, 2827
Van91I CCANNNNNTGG 3 cut(s) 578, 2074, 2720
VpaK11BI GGWCC 2 cut(s) 1646, 2757
XagI CCTNNNNNAGG 1 cut(s) 1727
XapI RAATTY 7 cut(s) 7, 130, 698, 1949, 2078, 2247, 2519
XceI RCATGY 5 cut(s) 60, 187, 2344, 2590, 2959
XmiI GTMKAC 1 cut(s) 2151
XmnI GAANNNNTTC 3 cut(s) 551, 1456, 2038
ZraI GACGTC 1 cut(s) 1519
ZrmI AGTACT 1 cut(s) 163
Zsp2I ATGCAT 1 cut(s) 2588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.