MD10G1223800.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012854
Physical Location & Seq
Forward (+)
32170520 .. 32175375
4856 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1223800.v1.1.491

Sequence Viewer

Length: 2964 bp
ATGGCGAATTCCGAAATGATGTCTGTCTTCCTCTGCTCTCTCTTTCTGTTCCTCACTTGTTTTTCTTCTGTTTCATCACTTAATCCGTCTCTCAGGAGGCAAGCATCAATTCTCGTTTCTCTCAAACAAAATTTTGAAGACTCGAAAAATCCTGCTTTAAGTACTTGGAATGTGTCAAACTACATGTTCCTCTGCTCTTGGGGTGGCATCAAGTGTGACAGCCGCAACAGATCAATTGTCTCACTTGACATATCCAACTACAATCTCTCGGGGACCCTTTCGCCAGTGATCACTGAACTCCGCACCCTTGTCAACGTTTCGGTGTCAGGAAATGGATTTTCTGGTATTTTTCCTCCCGGTATCCACAAACTAGCCGGGCTACAAAACCTCAACATCTCCAACAACGGGTTCGGTGGAAGCTTGGATTTGGAGTTTTCTAAGTTGGAGGAGCTTGTAATGCTAGATGCTTACAACAACGATTTCAATGGATCACTTCCATTAGGTGTCACTCAGCTTTCGAGGCTGAAGAGGTTGAACTTTGGCGGGAACTACTTCAGTGGGAGCATTCCTCCTAGCTATGGAAACATGGTGCAGCTTAATTATCTGTCAATTGCGGGAAATGACCTGAGCGGTTACATACCGAGCGAGCTCGGAAACCTCACTAACCTACAGCAGCTTTTGTTGGGATATTACAACGAATTTGAAGGCGGAATCCCACCAGAAATTGGCAAGCTGATCAATCTGTTTCATTTAGACCTCGCAAACTGCGGTTTGGAGGGGCCAATCCCTCCGGAGCTAGGCAATCTGAAAAAGCTAGACACTTTGTTCTTGCAAACAAACCAGCTCAGCGGTTCGGTTCCTGCTCAGCTGGGAAACTTGAGTAGCTTGAGGTCTCTTGATCTTTCGAACAATGCGCTGACGGGAGATATCCCCGCTGAGTTCTCTAGCCTCCGCGAGCTCACACTCCTGAACCTCTTCATCAACAAGTTTCACGGTGAGATTCCTCGCTCTATCGCGGAGCTACCAAACTTGGAAGTCTTGAAGCTGTGGCATAACAACTTCACCGGAGCCATACCTTCAAAGCTTGGTCAGAATGGTAAACTGATTGAGCTTGATCTTTCGAGTAACAAGCTCACCGGGGTTGTCCCAAAATCTCTTTGCTTTGGGAGGCGGCTGAAGATCTTAATTCTGCTCAACAATTTTCTCTTTGGGCCTCTACCTGATGACCTTGGAAAATGTGACACTCTAGTAAGAGTCCGAATGGGGCAGAATTATCTCACCGGATCAATCCCGCCGGGCTTTCTTTACTTGCCAGAGCTCTCACTAGTAGAGCTACAGAACAATTATCTCACCGGACAGCTTCTGCAGGAAAGAAGTAAACAACCCTCAAAACTCAGTCAGCTGAATCTGTCAAGCAACCGCTTATCCGGGCACCTTCCTGCATCTATTGGAAACTTCTCAAGCCTGCAGATTCTTCTATTGAGTGGAAATCAATTCACTGGAGAAATCCCATCTGACATAAGCCGGTTGCAAAGCGTTCTCAAGTTGGATATGAGCAGAAACAATTTTTCGGGCACAATACCTCCGGAAATTGGAGACTGTCTCGCTTTAACCTACTTAGATTTGAGCCAAAACCAACTCTCAGGTCCAATCCCAGTTCCGATTGTTCAAATCCACATACTGAATTACTTCAACGTGTCGTGGAACCAGTTAAACCAGAGCCTCCCAAAGGAGCTAGGCTCCTTGAAAAGCCTAACGTCTGCTGATTTTTCGCACAATAACTTCTCCGGTCCAATCCCACAAACAGGGCAATACTTGTTCTTCAACTCCACATCCTTTGTCGGTAACCCTGAGCTCTGCGATTCTTCTGCGAATCCATGCAACTACTCCTCAGCCTCATCATCAAAGGATCACAACCAAACCGGCACGCGCTCTCATGTCCTTGGCAAATTCAAGCTTGTCTTTGCTCTAGGACTTTTGCTTTGCTCGTTCGTGTTTGCAACTTTCGCAATCATCAAGACCAGAAAGGTGCGAAGAAATTCGAACTCGTGGAAGCTCACAGCATTCCAAAAGCTTGAATTCGGAAGCGAAGACATCTTAGAGTGCGTAAAGGAGAACAATGTGATAGGGAAAGGTGGAGCCGGGATTGTCTACAGAGGAACAATGTCGAGCGGCGAGCAAGTAGCAGTCAAGAAGCTGTTGGGGATCAACAAAGGATCGTCACACGACAATGGCCTCTCTGCAGAAATTCAGACACTGGGAAAAATCCGGCACCGGAACATTGTCCGATTGCTGGCGTTCTGTTCAAATAAAGAGACCAATCTGCTGGTTTACGAGTACATGCCGAATGGAAGCTTGGGTGAAGTTTTACATGGGAAGAGAGGAGGGTATCTCACGTGGGAGACTAGGTTGAACATAGCCATTGAAGCAGCAAAAGGGCTGTGTTATCTGCACCATGATTGCTCACCATTGATTCTTCATAGGGATGTGAAGTCCAACAACATTCTGCTCAACTCAGAATTTGAGGCTCATGTTGCAGATTTTGGGCTTGCCAAGTTCTTGCAGGACACTGGAACATCAGAATGCATGTCTGCAGTTGCTGGCTCATACGGATACATTGCTCCAGAATATGCGTACACATTGAAAGTTGATGAGAAGAGCGATGTGTATAGCTTTGGAGTTGTACTGTTGGAGCTCATCACAGGAAGAAGGCCAGTTGGTGGCTTTGGGGAAGAAGGAATGGACATTGTTCAGTGGACCAAGATTCAGACCAACTCACAGAAAGAAGGGGTGATAAAGATCCTCGACAAACGACTAGACACTGTTCCGATGGACGAAGCAATGCAAGTGTTTTTCGTCGCGGTTTTATGCGTTCAAGAGCACAGTGTGGAGAGACCGACCATGAGAGAAGTTGTTCAAATGCTTGCACAGGCTAAACAACCAAACACATTTCACATGCAATGA

Protein Analysis

988

Amino Acids

108.42

Weight (kDa)

6.82

Isoelectric Point (pI)

37.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 73 5.1e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 121 - 228 3.3e-08 Leucine-rich repeat region
LRR_14 PF23598 238 - 328 2.7e-08 Leucine-rich repeat region
LRR_8 PF13855 271 - 329 5.6e-07 Leucine rich repeat
LRR_8 PF13855 342 - 400 1.9e-06 Leucine rich repeat
Pkinase PF00069 705 - 973 3.4e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 705 - 975 5.2e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012854)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20270
fragaria_vesca FvH4_3g13410
malus_domestica MD05G1241900.v1.1 MD10G1223800.v1.1
prunus_persica Prupe.4G121500_v2.0.a1
pyrus_communis pycom05g21850 pycom10g18770 pycom10g18780
rosa_chinensis RchiOBHm_Chr5g0022141
rosa_laevigata RLG00000032647
rosa_multiflora Rmu_sc0000491.1_g000007
rosa_roxburghii Rroxscaffold_1G00056790
rosa_rugosa Rorug05G0071500.1
rosa_samantha Rh5AG159600 Rh5BG159100 Rh5CG173300 Rh5DG159100
rosa_wichuraiana Rw5G014250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1431, 2271
AccB7I CCANNNNNTGG 2 cut(s) 725, 2720
AccBSI CCGCTC 2 cut(s) 630, 2172
AccI GTMKAC 1 cut(s) 2151
AccII CGCG 4 cut(s) 954, 1016, 1930, 2861
AccIII TCCGGA 2 cut(s) 790, 1585
AclI AACGTT 1 cut(s) 315
AclWI GGATC 6 cut(s) 496, 1291, 1917, 2213, 2224, 2794
AcsI RAATTY 8 cut(s) 7, 130, 698, 1949, 2038, 2078, 2247, 2519
AcuI CTGAAG 3 cut(s) 538, 545, 1196
AcvI CACGTG 1 cut(s) 2397
AdeI CACNNNGTG 1 cut(s) 2887
AfaI GTAC 4 cut(s) 163, 2339, 2636, 2685
AfiI CCNNNNNNNGG 9 cut(s) 405, 578, 725, 797, 1592, 1729, 1805, 2293, 2720
AflIII ACRYGT 2 cut(s) 183, 1695
AhlI ACTAGT 1 cut(s) 1324
AjuI GAANNNNNNNTTGG 4 cut(s) 392, 424, 2339, 2371
Alw21I GWGCWC 6 cut(s) 651, 960, 1320, 1857, 2697, 2883
Alw26I GTCTC 8 cut(s) 93, 244, 897, 1590, 1607, 2309, 2396, 2887
AlwI GGATC 6 cut(s) 496, 1291, 1917, 2213, 2224, 2794
AlwNI CAGNNNCTG 3 cut(s) 1363, 2257, 2600
Ama87I CYCGRG 1 cut(s) 268
Aor13HI TCCGGA 2 cut(s) 790, 1585
AoxI GGCC 4 cut(s) 779, 1211, 2233, 2711
ApeKI GCWGC 3 cut(s) 592, 673, 2429
ApoI RAATTY 8 cut(s) 7, 130, 698, 1949, 2038, 2078, 2247, 2519
ArsI GACNNNNNNTTYG 2 cut(s) 1965, 1997
Asp700I GAANNNNTTC 5 cut(s) 551, 974, 1688, 2038, 2913
AspLEI GCGC 2 cut(s) 916, 1932
AspS9I GGNCC 6 cut(s) 273, 779, 1211, 1646, 1790, 2757
AsuC2I CCSGG 6 cut(s) 357, 376, 1138, 1296, 1429, 2143
AsuHPI GGTGA 8 cut(s) 1007, 1054, 1126, 1270, 1342, 2372, 2457, 2803
AsuII TTCGAA 2 cut(s) 905, 2042
AvaI CYCGRG 1 cut(s) 268
AvaII GGWCC 4 cut(s) 273, 1646, 1790, 2757
BaeGI GKGCMC 2 cut(s) 1434, 1577
BanI GGYRCC 2 cut(s) 1431, 2271
BanII GRGCYC 5 cut(s) 651, 960, 1320, 1857, 2697
BauI CACGAG 1 cut(s) 2047
BbrPI CACGTG 1 cut(s) 2397
BbsI GAAGAC 3 cut(s) 19, 144, 2097
Bbv12I GWGCWC 6 cut(s) 651, 960, 1320, 1857, 2697, 2883
BbvCI CCTCAGC 1 cut(s) 1891
BbvI GCAGC 3 cut(s) 604, 685, 2441
BccI CCATC 2 cut(s) 1519, 2824
BcgI CGANNNNNNTGC 2 cut(s) 1850, 1884
BciVI GTATCC 2 cut(s) 371, 2606
BclI TGATCA 2 cut(s) 288, 735
BcnI CCSGG 6 cut(s) 357, 376, 1138, 1296, 1429, 2143
BcoDI GTCTC 8 cut(s) 93, 244, 897, 1590, 1607, 2309, 2396, 2887
BcuI ACTAGT 1 cut(s) 1324
BfmI CTRYAG 7 cut(s) 668, 1334, 1364, 1466, 2152, 2241, 2592
BfuI GTATCC 2 cut(s) 371, 2606
BglII AGATCT 1 cut(s) 1179
BisI GCNGC 6 cut(s) 223, 593, 674, 1172, 2173, 2430
BlpI GCTNAGC 2 cut(s) 845, 864
BlsI GCNGC 6 cut(s) 224, 594, 675, 1173, 2174, 2431
BmcAI AGTACT 1 cut(s) 163
Bme1390I CCNGG 6 cut(s) 357, 376, 1138, 1296, 1429, 2143
Bme18I GGWCC 4 cut(s) 273, 1646, 1790, 2757
BmeT110I CYCGRG 1 cut(s) 268
BmgT120I GGNCC 6 cut(s) 273, 779, 1211, 1646, 1790, 2757
BmrFI CCNGG 6 cut(s) 357, 376, 1138, 1296, 1429, 2143
BmrI ACTGGG 2 cut(s) 1649, 2267
BmsI GCATC 4 cut(s) 113, 216, 454, 1451
BmuI ACTGGG 2 cut(s) 1649, 2267
BpiI GAAGAC 3 cut(s) 19, 144, 2097
BplI GAGNNNNNCTC 8 cut(s) 553, 585, 1587, 1619, 1724, 1756, 2376, 2408
BpmI CTGGAG 2 cut(s) 1521, 2607
Bpu10I CCTNAGC 3 cut(s) 626, 1851, 1891
Bpu1102I GCTNAGC 2 cut(s) 845, 864
Bpu14I TTCGAA 2 cut(s) 905, 2042
BpuEI CTTGAG 4 cut(s) 898, 907, 1444, 1526
BpuMI CCSGG 6 cut(s) 357, 376, 1138, 1296, 1429, 2143
BsaAI YACGTR 1 cut(s) 2397
BsaBI GATNNNNATC 1 cut(s) 2798
BsaI GGTCTC 3 cut(s) 897, 2309, 2887
BsaJI CCNNGG 3 cut(s) 1137, 1228, 1942
BsaWI WCCGGW 7 cut(s) 790, 1064, 1280, 1352, 1585, 1787, 2274
BsaXI ACNNNNNCTCC 4 cut(s) 1567, 1597, 2393, 2423
Bsc4I CCNNNNNNNGG 9 cut(s) 405, 578, 725, 797, 1592, 1729, 1805, 2293, 2720
Bse118I RCCGGY 2 cut(s) 1524, 1922
Bse1I ACTGG 7 cut(s) 284, 1504, 1655, 1708, 2262, 2575, 2714
Bse3DI GCAATG 2 cut(s) 2616, 2847
Bse8I GATNNNNATC 1 cut(s) 2798
BseAI TCCGGA 2 cut(s) 790, 1585
BseDI CCNNGG 3 cut(s) 1137, 1228, 1942
BseGI GGATG 2 cut(s) 1832, 2491
BseJI GATNNNNATC 1 cut(s) 2798
BseLI CCNNNNNNNGG 9 cut(s) 405, 578, 725, 797, 1592, 1729, 1805, 2293, 2720
BseMI GCAATG 2 cut(s) 2616, 2847
BseNI ACTGG 7 cut(s) 284, 1504, 1655, 1708, 2262, 2575, 2714
BseRI GAGGAG 3 cut(s) 461, 1879, 2397
BseSI GKGCMC 2 cut(s) 1434, 1577
BseXI GCAGC 3 cut(s) 604, 685, 2441
BseYI CCCAGC 1 cut(s) 868
BsgI GTGCAG 2 cut(s) 611, 2435
Bsh1236I CGCG 4 cut(s) 954, 1016, 1930, 2861
BshFI GGCC 4 cut(s) 781, 1213, 2235, 2713
BshNI GGYRCC 2 cut(s) 1431, 2271
BsiHKAI GWGCWC 6 cut(s) 651, 960, 1320, 1857, 2697, 2883
BsiHKCI CYCGRG 1 cut(s) 268
BslFI GGGAC 2 cut(s) 286, 1130
BslI CCNNNNNNNGG 9 cut(s) 405, 578, 725, 797, 1592, 1729, 1805, 2293, 2720
BsmAI GTCTC 8 cut(s) 93, 244, 897, 1590, 1607, 2309, 2396, 2887
BsmBI CGTCTC 1 cut(s) 93
BsmFI GGGAC 2 cut(s) 286, 1130
BsmI GAATGC 3 cut(s) 564, 2063, 2588
BsnI GGCC 4 cut(s) 781, 1213, 2235, 2713
Bso31I GGTCTC 3 cut(s) 897, 2309, 2887
BsoBI CYCGRG 1 cut(s) 268
Bsp119I TTCGAA 2 cut(s) 905, 2042
Bsp1286I GDGCHC 8 cut(s) 651, 960, 1320, 1434, 1577, 1857, 2697, 2883
Bsp13I TCCGGA 2 cut(s) 790, 1585
Bsp1720I GCTNAGC 2 cut(s) 845, 864
BspANI GGCC 4 cut(s) 781, 1213, 2235, 2713
BspEI TCCGGA 2 cut(s) 790, 1585
BspFNI CGCG 4 cut(s) 954, 1016, 1930, 2861
BspMAI CTGCAG 4 cut(s) 1368, 1470, 2245, 2596
BspPI GGATC 6 cut(s) 496, 1291, 1917, 2213, 2224, 2794
BspQI GCTCTTC 1 cut(s) 2651
BspT104I TTCGAA 2 cut(s) 905, 2042
BspT107I GGYRCC 2 cut(s) 1431, 2271
BspTNI GGTCTC 3 cut(s) 897, 2309, 2887
BsrBI CCGCTC 2 cut(s) 630, 2172
BsrDI GCAATG 2 cut(s) 2616, 2847
BsrFI RCCGGY 2 cut(s) 1524, 1922
BsrI ACTGG 7 cut(s) 284, 1504, 1655, 1708, 2262, 2575, 2714
BssAI RCCGGY 2 cut(s) 1524, 1922
BssECI CCNNGG 3 cut(s) 1137, 1228, 1942
BssSI CACGAG 1 cut(s) 2047
BssT1I CCWWGG 2 cut(s) 1228, 1942
Bst2BI CACGAG 1 cut(s) 2047
Bst4CI ACNGT 5 cut(s) 995, 1601, 2688, 2824, 2885
Bst6I CTCTTC 4 cut(s) 521, 980, 2372, 2651
BstBAI YACGTR 1 cut(s) 2397
BstBI TTCGAA 2 cut(s) 905, 2042
BstEII GGTNACC 1 cut(s) 1844
BstENI CCTNNNNNAGG 1 cut(s) 1727
BstF5I GGATG 2 cut(s) 1832, 2491
BstFNI CGCG 4 cut(s) 954, 1016, 1930, 2861
BstHHI GCGC 2 cut(s) 916, 1932
BstMAI GTCTC 8 cut(s) 93, 244, 897, 1590, 1607, 2309, 2396, 2887
BstMWI GCNNNNNNNGC 5 cut(s) 457, 520, 2006, 2426, 2534
BstNSI RCATGY 4 cut(s) 187, 2344, 2590, 2959
BstPI GGTNACC 1 cut(s) 1844
BstSCI CCNGG 6 cut(s) 355, 374, 1136, 1294, 1427, 2141
BstSFI CTRYAG 7 cut(s) 668, 1334, 1364, 1466, 2152, 2241, 2592
BstSLI GKGCMC 2 cut(s) 1434, 1577
BstUI CGCG 4 cut(s) 954, 1016, 1930, 2861
BstV1I GCAGC 3 cut(s) 604, 685, 2441
BstV2I GAAGAC 3 cut(s) 19, 144, 2097
BstX2I RGATCY 2 cut(s) 1179, 2799
BstXI CCANNNNNNTGG 1 cut(s) 2326
BstYI RGATCY 2 cut(s) 1179, 2799
BsuI GTATCC 2 cut(s) 371, 2606
BsuRI GGCC 4 cut(s) 781, 1213, 2235, 2713
BtgZI GCGATG 1 cut(s) 2676
BtsCI GGATG 2 cut(s) 1832, 2491
BtsIMutI CAGTG 9 cut(s) 291, 291, 562, 1497, 2255, 2568, 2759, 2820, 2890
CaiI CAGNNNCTG 3 cut(s) 1363, 2257, 2600
CfoI GCGC 2 cut(s) 916, 1932
Cfr10I RCCGGY 2 cut(s) 1524, 1922
Cfr13I GGNCC 6 cut(s) 273, 779, 1211, 1646, 1790, 2757
Csp6I GTAC 4 cut(s) 162, 2338, 2635, 2684
CspCI CAANNNNNGTGG 2 cut(s) 1819, 1854
CviQI GTAC 4 cut(s) 162, 2338, 2635, 2684
DraIII CACNNNGTG 1 cut(s) 2887
Eam1104I CTCTTC 4 cut(s) 521, 980, 2372, 2651
EarI CTCTTC 4 cut(s) 521, 980, 2372, 2651
EciI GGCGGA 1 cut(s) 723
Ecl136II GAGCTC 5 cut(s) 649, 958, 1318, 1855, 2695
Eco130I CCWWGG 2 cut(s) 1228, 1942
Eco24I GRGCYC 5 cut(s) 651, 960, 1320, 1857, 2697
Eco31I GGTCTC 3 cut(s) 897, 2309, 2887
Eco32I GATATC 1 cut(s) 928
Eco47I GGWCC 4 cut(s) 273, 1646, 1790, 2757
Eco53kI GAGCTC 5 cut(s) 649, 958, 1318, 1855, 2695
Eco57I CTGAAG 3 cut(s) 538, 545, 1196
Eco72I CACGTG 1 cut(s) 2397
Eco88I CYCGRG 1 cut(s) 268
Eco91I GGTNACC 1 cut(s) 1844
EcoICRI GAGCTC 5 cut(s) 649, 958, 1318, 1855, 2695
EcoNI CCTNNNNNAGG 1 cut(s) 1727
EcoO109I RGGNCCY 1 cut(s) 273
EcoO65I GGTNACC 1 cut(s) 1844
EcoRI GAATTC 2 cut(s) 7, 2078
EcoRV GATATC 1 cut(s) 928
EcoT14I CCWWGG 2 cut(s) 1228, 1942
EcoT22I ATGCAT 1 cut(s) 2588
EcoT38I GRGCYC 5 cut(s) 651, 960, 1320, 1857, 2697
ErhI CCWWGG 2 cut(s) 1228, 1942
Esp3I CGTCTC 1 cut(s) 93
FalI AAGNNNNNCTT 2 cut(s) 1946, 1978
FaqI GGGAC 2 cut(s) 286, 1130
FauI CCCGC 4 cut(s) 536, 607, 940, 1299
FbaI TGATCA 2 cut(s) 288, 735
FblI GTMKAC 1 cut(s) 2151
Fnu4HI GCNGC 6 cut(s) 223, 593, 674, 1172, 2173, 2430
FokI GGATG 2 cut(s) 1819, 2498
FriOI GRGCYC 5 cut(s) 651, 960, 1320, 1857, 2697
Fsp4HI GCNGC 6 cut(s) 223, 593, 674, 1172, 2173, 2430
GlaI GCGC 2 cut(s) 915, 1931
GluI GCNGC 6 cut(s) 223, 593, 674, 1172, 2173, 2430
GsaI CCCAGC 1 cut(s) 872
GsuI CTGGAG 2 cut(s) 1521, 2607
HaeIII GGCC 4 cut(s) 781, 1213, 2235, 2713
HhaI GCGC 2 cut(s) 916, 1932
Hin6I GCGC 2 cut(s) 914, 1930
HinP1I GCGC 2 cut(s) 914, 1930
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HindIII AAGCTT 5 cut(s) 418, 1082, 1955, 2072, 2353
HphI GGTGA 8 cut(s) 1007, 1054, 1126, 1270, 1342, 2372, 2457, 2803
Hpy166II GTNNAC 7 cut(s) 313, 1100, 1379, 2152, 2332, 2637, 2757
Hpy8I GTNNAC 7 cut(s) 313, 1100, 1379, 2152, 2332, 2637, 2757
Hpy99I CGWCG 1 cut(s) 2861
HpyAV CCTTC 6 cut(s) 698, 1086, 1445, 2703, 2729, 2780
HpyCH4III ACNGT 5 cut(s) 995, 1601, 2688, 2824, 2885
HpyCH4IV ACGT 4 cut(s) 315, 1695, 1757, 2396
HpyF10VI GCNNNNNNNGC 5 cut(s) 457, 520, 2006, 2426, 2534
HpySE526I ACGT 4 cut(s) 315, 1695, 1757, 2396
HspAI GCGC 2 cut(s) 914, 1930
KflI GGGWCCC 1 cut(s) 273
Kpn2I TCCGGA 2 cut(s) 790, 1585
Ksp22I TGATCA 2 cut(s) 288, 735
LguI GCTCTTC 1 cut(s) 2651
Lsp1109I GCAGC 3 cut(s) 604, 685, 2441
LweI GCATC 4 cut(s) 113, 216, 454, 1451
MaeII ACGT 4 cut(s) 315, 1695, 1757, 2396
MaeIII GTNAC 7 cut(s) 215, 505, 632, 1124, 1238, 1844, 2220
MbiI CCGCTC 2 cut(s) 630, 2172
MfeI CAATTG 2 cut(s) 234, 609
MflI RGATCY 2 cut(s) 1179, 2799
MhlI GDGCHC 8 cut(s) 651, 960, 1320, 1434, 1577, 1857, 2697, 2883
MlyI GAGTC 2 cut(s) 134, 1263
MmeI TCCRAC 6 cut(s) 279, 423, 423, 1527, 2520, 2670
Mph1103I ATGCAT 1 cut(s) 2588
MroI TCCGGA 2 cut(s) 790, 1585
MroXI GAANNNNTTC 5 cut(s) 551, 974, 1688, 2038, 2913
MseI TTAA 6 cut(s) 81, 158, 597, 1184, 1610, 1712
MslI CAYNNNNRTG 3 cut(s) 2229, 2484, 2581
MspA1I CMGCKG 4 cut(s) 849, 868, 935, 1402
MspR9I CCNGG 6 cut(s) 357, 376, 1138, 1296, 1429, 2143
MunI CAATTG 2 cut(s) 234, 609
Mva1269I GAATGC 3 cut(s) 564, 2063, 2588
MvnI CGCG 4 cut(s) 954, 1016, 1930, 2861
MwoI GCNNNNNNNGC 5 cut(s) 457, 520, 2006, 2426, 2534
NciI CCSGG 6 cut(s) 357, 376, 1138, 1296, 1429, 2143
NmuCI GTSAC 4 cut(s) 215, 505, 1238, 2220
NsiI ATGCAT 1 cut(s) 2588
NspI RCATGY 4 cut(s) 187, 2344, 2590, 2959
NspV TTCGAA 2 cut(s) 905, 2042
PciI ACATGT 1 cut(s) 183
PciSI GCTCTTC 1 cut(s) 2651
PctI GAATGC 3 cut(s) 564, 2063, 2588
PdmI GAANNNNTTC 5 cut(s) 551, 974, 1688, 2038, 2913
PfeI GAWTC 8 cut(s) 711, 1000, 1405, 1471, 1862, 1873, 2473, 2764
PflMI CCANNNNNTGG 2 cut(s) 725, 2720
PkrI GCNGC 6 cut(s) 224, 594, 675, 1173, 2174, 2431
PleI GAGTC 2 cut(s) 134, 1262
PmaCI CACGTG 1 cut(s) 2397
PmlI CACGTG 1 cut(s) 2397
PpsI GAGTC 2 cut(s) 134, 1262
Ppu21I YACGTR 1 cut(s) 2397
PpuMI RGGWCCY 1 cut(s) 273
PscI ACATGT 1 cut(s) 183
Psp124BI GAGCTC 5 cut(s) 651, 960, 1320, 1857, 2697
Psp1406I AACGTT 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 273
PspCI CACGTG 1 cut(s) 2397
PspEI GGTNACC 1 cut(s) 1844
PspFI CCCAGC 1 cut(s) 868
PspPI GGNCC 6 cut(s) 273, 779, 1211, 1646, 1790, 2757
PspPPI RGGWCCY 1 cut(s) 273
PstI CTGCAG 4 cut(s) 1368, 1470, 2245, 2596
PstNI CAGNNNCTG 3 cut(s) 1363, 2257, 2600
PsuI RGATCY 2 cut(s) 1179, 2799
PvuII CAGCTG 2 cut(s) 868, 1402
RsaI GTAC 4 cut(s) 163, 2339, 2636, 2685
RsaNI GTAC 4 cut(s) 162, 2338, 2635, 2684
RseI CAYNNNNRTG 3 cut(s) 2229, 2484, 2581
SacI GAGCTC 5 cut(s) 651, 960, 1320, 1857, 2697
SapI GCTCTTC 1 cut(s) 2651
SaqAI TTAA 6 cut(s) 81, 158, 597, 1184, 1610, 1712
SatI GCNGC 6 cut(s) 223, 593, 674, 1172, 2173, 2430
Sau96I GGNCC 6 cut(s) 273, 779, 1211, 1646, 1790, 2757
ScaI AGTACT 1 cut(s) 163
SchI GAGTC 2 cut(s) 134, 1263
ScrFI CCNGG 6 cut(s) 357, 376, 1138, 1296, 1429, 2143
SduI GDGCHC 8 cut(s) 651, 960, 1320, 1434, 1577, 1857, 2697, 2883
SfaNI GCATC 4 cut(s) 113, 216, 454, 1451
SfcI CTRYAG 7 cut(s) 668, 1334, 1364, 1466, 2152, 2241, 2592
SfuI TTCGAA 2 cut(s) 905, 2042
SinI GGWCC 4 cut(s) 273, 1646, 1790, 2757
SmiMI CAYNNNNRTG 3 cut(s) 2229, 2484, 2581
SmlI CTYRAG 4 cut(s) 877, 886, 1459, 1541
SmoI CTYRAG 4 cut(s) 877, 886, 1459, 1541
SpeI ACTAGT 1 cut(s) 1324
SstI GAGCTC 5 cut(s) 651, 960, 1320, 1857, 2697
StyD4I CCNGG 6 cut(s) 355, 374, 1136, 1294, 1427, 2141
StyI CCWWGG 2 cut(s) 1228, 1942
TaaI ACNGT 5 cut(s) 995, 1601, 2688, 2824, 2885
TaiI ACGT 4 cut(s) 318, 1698, 1760, 2399
TaqI TCGA 7 cut(s) 143, 518, 905, 1121, 2042, 2168, 2805
TaqII GACCGA 1 cut(s) 2911
TatI WGTACW 3 cut(s) 161, 2337, 2683
TauI GCSGC 3 cut(s) 225, 1174, 2175
TfiI GAWTC 8 cut(s) 711, 1000, 1405, 1471, 1862, 1873, 2473, 2764
Tru1I TTAA 6 cut(s) 81, 158, 597, 1184, 1610, 1712
Tru9I TTAA 6 cut(s) 81, 158, 597, 1184, 1610, 1712
TscAI CASTG 9 cut(s) 291, 298, 562, 1504, 2262, 2575, 2759, 2827, 2890
TseFI GTSAC 4 cut(s) 215, 505, 1238, 2220
TseI GCWGC 3 cut(s) 592, 673, 2429
Tsp45I GTSAC 4 cut(s) 215, 505, 1238, 2220
TspDTI ATGAA 4 cut(s) 63, 737, 967, 2468
TspGWI ACGGA 2 cut(s) 75, 2625
TspRI CASTG 9 cut(s) 291, 298, 562, 1504, 2262, 2575, 2759, 2827, 2890
Van91I CCANNNNNTGG 2 cut(s) 725, 2720
VpaK11BI GGWCC 4 cut(s) 273, 1646, 1790, 2757
XagI CCTNNNNNAGG 1 cut(s) 1727
XapI RAATTY 8 cut(s) 7, 130, 698, 1949, 2038, 2078, 2247, 2519
XceI RCATGY 4 cut(s) 187, 2344, 2590, 2959
XmiI GTMKAC 1 cut(s) 2151
XmnI GAANNNNTTC 5 cut(s) 551, 974, 1688, 2038, 2913
ZrmI AGTACT 1 cut(s) 163
Zsp2I ATGCAT 1 cut(s) 2588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.