Rw5G014250
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0012854
Physical Location & Seq
Reverse (-)
16932982 .. 16937319
4338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G014250.1

Sequence Viewer

Length: 2949 bp
ATGAAGTCCGTCTTCCTCTCCTCTCTTCTTGTGTTCTTCATTTGCTTCCGTTGTGTTTGTTCACATAATCAGTCTCCTAGGAGACAAGCTTCAATCTTGGTTTCTCTCAAGCAAGGTTTTGAAGCCTCAGATCCTTCTCTAAGTAGCTGGAATGTATCAAACTATATGTTCCTTTGCTCTTGGGCTGGTATCAAATGTGACAACCTCAGCAGATCAGTAGTTTCCATAGACATATCAAATTACAACCTCTCCGGTTCACTTTCTCCTGCGATCACAGAACTCAGAACCCTTGTCAATCTCTCGGTGTCTGGGAATGATTTTTCAGGTAGTTTCCCAGCCGGAATTCACAAGCTTCCAAGGCTTCAATACCTCAACATATCCAACAATGGCTTTGGTGGAAGCTTGGACTGGGAATTTGGTCTGCTAAAGGAGCTCATATTGCTCGATGCTTACAACAATGATTTCAATGGTTCACTCCCATTAGGTGTCACTCAACTTCCCAAGCTGAAGCGCTTAGACTTCGGAGGAAATTACTTTAGTGGAAGCATCCCTCCTAGCTATGGAAATATGTCACAGCTGAATTATCTCTCAGTTGCAGGAAATGATCTGAGTGGTTTCATACCAAGTGAGCTTGGCAATCTTACTAGCCTCAATCAGCTGTTCTTGGGGTATTACAACGATTTCGATGGTGGAATCCCACCCGAGATTGGTAAGCTGACCAATCTGTTCCATTTAGACCTTGCAAACTGTGGCTTGGAGGGCTCAATGCCACCTGAACTGGGAAATCTGAAGAAGCTAGACACCTTGTTCTTGCAAACCAATCAGCTCAGCGGTTCCATTCCTGCTCAGCTTGGGAATTTGAGTAGCTTGAGGTCTCTTGATCTCTCAAACAATGCACTAACAGGAGACATTCCCGTCGAGTTCTCAGCCCTCCGCGAGCTTACTCTCTTGAACTTGTTCATCAACAAGTTTCACGGAGAGATCCCTCATGAAATAGCTGAGCTACCCAACTTGGAGGTCTTGAAGCTATGGCAAAACAATTTCACTGGAGCAATTCCTTCAAAGCTTGGTCAGAATGGTAAAATGATTGAGCTTGATCTGTCTACTAATAAGCTCACTGGGGAAATCCCAAAATCTCTTTGTTTTGGAAGGAAGCTGAAGATCTTGATTTTGCTCAACAATTTTCTCTTTGGGCCTCTACCGGATGATCTTGGTCACTGTGACACTCTAGTAAGAGTTAGGCTGGGGCAGAATTACCTGAGTGGATCAATTCCAAGAGGGTTTCTTTACTTGCCTGAGCTCGCTCTTATGGAGCTGCAGAACAATTATCTTACCGGTCGACTTGATATGCAGGGAAAAAGCCAAGTGCCCTCAAAGCTTTCACAGCTGAATCTCTCAAGTAATCGCTTATCGGGGTCACCTCCTGCATCTATTGGAAACTTTTCTAGCTTGCAGATTCTTTTGCTAAATGGAAACAGATTTACTGGAGAAATTCCATCTGACATAGGCCGGTTGAGAAATGTTCTCAAGTTGGATATGAGCAGAAACAATTTTTCGGGCAGCATCCCTCCCGAAATTGGAAATTGTACCTTGCTGACGTATCTAGATTTGAGCCAAAACCAACTTTCTGGTCCAATTCCACCTCAAGTAGTTCAAATTCACATACTGAATTACTTCAACGTGTCATGGAACCACTTAAACCAGAGCCTACCTAAGGAACTTGGGTCCTTGAAGAGTCTAACCTCTGCTGATTTTTCCCACAATAGTTTCTCAGGTTCAGTACCACAAACAGGTCAATACTTATTCTTTAACTCCACATCTTTTATAGGCAACCCTGATTTATGTGACTCTTCAGTCAATCCTTGCAACTACTCTTCGAACTCACCATTAGAGTCTCACAACCAAAATGGCACAAGGTCTCAAGTCCTTGGGAAATTCAAGCTTGTGTTTGCATTAGGCCTCTTGTTGTGTTCATTTGTGTTTGCAACCTTTGCAATCATAAAGACCAGAAAGGTGAGGAAGAATTCGAACACATGGAAGCTCACCGCCTTCCAGAAGCTAGAATTTGGAAGCGAACACATCTTGGAGTGCATCAAGGACAACAATGTTATAGGGAGAGGTGGTGCTGGTATTGTATATAGAGGAACGATGTTGAGCGGCGAGCAAGTAGCTGTGAAGAAGCTGTTGGGGATTAACAAAGGCTCTTCTCATGATAATGGCCTCTCTGCAGAAATTCAGACACTTGGGAAAATCAGGCACCGGAACATTGTCCGGTTGCTAGCATTCTGCTCAAACAAAGAGACCAATTTGCTTGTTTATGAGTACATGCCGAATGGGAGCTTGGGTGAAGTTTTGCATGGGAAGAGAGGTGGATATCTAAAATGGGAGACTAGGCTTAACATAGCCATTGAAGCAGCGAAAGGGCTTTGTTACTTGCACCATGATTGTTCCCCTTTGATTCTTCATAGGGATGTTAAGTCCAACAACATTTTGCTCAACTCGGAGTTCGAGGCTCATGTAGCAGATTTTGGGCTTGCCAAGTTCTTGCAGGACACTGGAACATCAGAATGCATGTCTGCAATTGCTGGCTCATATGGTTACATTGCTCCAGAGTATGCATACACATTGAGAGTTGATGCGAAGAGCGATGTGTATAGCTTCGGGGTGGTACTATTGGAACTGATCACAGGGAGGAGGCCAGTAGGAGGTTTCGGAGAAGAGGGCATGGACATTGTTCAATGGACTAAGGTACAGACTGACTCGCGCAAAGAAGGGGTGATCAAGATCCTAGATCAACGGCTAGGAAGTGTTCCCTTGGACGAAGCAATGCAGGTGTTCTTTGTAGCAGTGTTATGTGTTCAAGAGCACAGTGTGGAGAGACCAACCATGAGAGAAGTTGTTCAAATGCTTGCACAAGCTAAGCAACCAAACACATTTCACATGCAATGA

Protein Analysis

982

Amino Acids

108.07

Weight (kDa)

7.53

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 67 7.8e-07 Leucine rich repeat N-terminal domain
LRR_14 PF23598 92 - 222 2.8e-09 Leucine-rich repeat region
LRR_14 PF23598 232 - 322 1.1e-08 Leucine-rich repeat region
LRR_8 PF13855 265 - 323 1.1e-06 Leucine rich repeat
LRR_ComC PF24141 286 - 381 9.3e-06 EGF-like domain-containing protein ComC LRR domain
Pkinase PF00069 700 - 968 6.8e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 701 - 970 3.5e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012854)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20270
fragaria_vesca FvH4_3g13410
malus_domestica MD05G1241900.v1.1 MD10G1223800.v1.1
prunus_persica Prupe.4G121500_v2.0.a1
pyrus_communis pycom05g21850 pycom10g18770 pycom10g18780
rosa_chinensis RchiOBHm_Chr5g0022141
rosa_laevigata RLG00000032647
rosa_multiflora Rmu_sc0000491.1_g000007
rosa_roxburghii Rroxscaffold_1G00056790
rosa_rugosa Rorug05G0071500.1
rosa_samantha Rh5AG159600 Rh5BG159100 Rh5CG173300 Rh5DG159100
rosa_wichuraiana Rw5G014250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2821
AasI GACNNNNNNGTC 2 cut(s) 914, 1853
Acc36I ACCTGC 1 cut(s) 2821
AccB1I GGYRCC 1 cut(s) 2256
AccBSI CCGCTC 1 cut(s) 2157
AccI GTMKAC 2 cut(s) 1103, 1339
AccII CGCG 2 cut(s) 936, 2764
AciI CCGC 4 cut(s) 831, 934, 2046, 2157
AclWI GGATC 4 cut(s) 125, 976, 1273, 2779
AcsI RAATTY 9 cut(s) 342, 413, 856, 1491, 1656, 1934, 2023, 2063, 2232
AcuI CTGAAG 4 cut(s) 527, 809, 1178, 1836
AdeI CACNNNGTG 1 cut(s) 2872
AfaI GTAC 5 cut(s) 1588, 1782, 2324, 2670, 2751
AfeI AGCGCT 1 cut(s) 512
AfiI CCNNNNNNNGG 7 cut(s) 560, 707, 779, 1577, 1714, 1790, 2705
AflIII ACRYGT 1 cut(s) 1680
AgeI ACCGGT 1 cut(s) 1334
AjuI GAANNNNNNNTTGG 4 cut(s) 2168, 2200, 2324, 2356
Alw21I GWGCWC 3 cut(s) 435, 1302, 2868
Alw26I GTCTC 9 cut(s) 76, 78, 879, 900, 1899, 1923, 2294, 2381, 2872
AlwI GGATC 4 cut(s) 125, 976, 1273, 2779
Ama87I CYCGRG 1 cut(s) 701
Aor51HI AGCGCT 1 cut(s) 512
AoxI GGCC 5 cut(s) 1193, 1507, 1957, 2218, 2696
ApeKI GCWGC 3 cut(s) 1315, 1560, 2414
ApoI RAATTY 9 cut(s) 342, 413, 856, 1491, 1656, 1934, 2023, 2063, 2232
ArsI GACNNNNNNTTYG 2 cut(s) 398, 430
AsiGI ACCGGT 1 cut(s) 1334
Asp700I GAANNNNTTC 4 cut(s) 956, 1441, 1673, 2898
AspA2I CCTAGG 1 cut(s) 77
AspLEI GCGC 2 cut(s) 513, 2766
AspS9I GGNCC 3 cut(s) 1193, 1631, 1725
AsuHPI GGTGA 6 cut(s) 1410, 1875, 2026, 2035, 2357, 2788
AsuII TTCGAA 2 cut(s) 1877, 2027
AsuNHI GCTAGC 1 cut(s) 2278
AvaI CYCGRG 1 cut(s) 701
AvaII GGWCC 2 cut(s) 1631, 1725
AvrII CCTAGG 1 cut(s) 77
AxyI CCTNAGG 1 cut(s) 1713
BaeGI GKGCMC 1 cut(s) 1371
BanI GGYRCC 1 cut(s) 2256
BanII GRGCYC 3 cut(s) 435, 764, 1302
BbsI GAAGAC 1 cut(s) 4
Bbv12I GWGCWC 3 cut(s) 435, 1302, 2868
BbvCI CCTCAGC 1 cut(s) 206
BbvI GCAGC 3 cut(s) 1302, 1572, 2426
BccI CCATC 2 cut(s) 680, 1504
BceAI ACGGC 1 cut(s) 2813
BclI TGATCA 2 cut(s) 2682, 2778
BcoDI GTCTC 9 cut(s) 76, 78, 879, 900, 1899, 1923, 2294, 2381, 2872
BfmI CTRYAG 2 cut(s) 1316, 2226
BfoI RGCGCY 1 cut(s) 514
BfuAI ACCTGC 1 cut(s) 2821
BglII AGATCT 1 cut(s) 1161
BisI GCNGC 4 cut(s) 1316, 1561, 2158, 2415
BlnI CCTAGG 1 cut(s) 77
BlpI GCTNAGC 4 cut(s) 827, 846, 999, 2919
BlsI GCNGC 4 cut(s) 1317, 1562, 2159, 2416
Bme18I GGWCC 2 cut(s) 1631, 1725
BmeT110I CYCGRG 1 cut(s) 701
BmgT120I GGNCC 3 cut(s) 1193, 1631, 1725
BmiI GGNNCC 4 cut(s) 835, 1691, 1726, 2258
BmrI ACTGGG 3 cut(s) 418, 788, 1128
BmsI GCATC 6 cut(s) 436, 555, 1436, 1572, 2100, 2626
BmtI GCTAGC 1 cut(s) 2282
BmuI ACTGGG 3 cut(s) 418, 788, 1128
BpiI GAAGAC 1 cut(s) 4
BpmI CTGGAG 3 cut(s) 1068, 1506, 2592
Bpu10I CCTNAGC 2 cut(s) 206, 1296
Bpu1102I GCTNAGC 4 cut(s) 827, 846, 999, 2919
Bpu14I TTCGAA 2 cut(s) 1877, 2027
BpuEI CTTGAG 6 cut(s) 92, 889, 1381, 1511, 1629, 1905
BsaBI GATNNNNATC 1 cut(s) 2783
BsaI GGTCTC 4 cut(s) 879, 1923, 2294, 2872
BsaJI CCNNGG 4 cut(s) 77, 356, 1927, 2814
BsaWI WCCGGW 5 cut(s) 251, 1201, 1334, 2259, 2271
BsaXI ACNNNNNCTCC 6 cut(s) 233, 263, 2107, 2137, 2329, 2359
Bsc4I CCNNNNNNNGG 7 cut(s) 560, 707, 779, 1577, 1714, 1790, 2705
Bse118I RCCGGY 2 cut(s) 1334, 1509
Bse1I ACTGG 7 cut(s) 413, 783, 1051, 1123, 1489, 2560, 2699
Bse21I CCTNAGG 1 cut(s) 1713
Bse3DI GCAATG 2 cut(s) 2601, 2832
Bse8I GATNNNNATC 1 cut(s) 2783
BseDI CCNNGG 4 cut(s) 77, 356, 1927, 2814
BseGI GGATG 4 cut(s) 546, 1210, 1563, 2476
BseJI GATNNNNATC 1 cut(s) 2783
BseLI CCNNNNNNNGG 7 cut(s) 560, 707, 779, 1577, 1714, 1790, 2705
BseMI GCAATG 2 cut(s) 2601, 2832
BseNI ACTGG 7 cut(s) 413, 783, 1051, 1123, 1489, 2560, 2699
BseRI GAGGAG 2 cut(s) 10, 2707
BseSI GKGCMC 1 cut(s) 1371
BseXI GCAGC 3 cut(s) 1302, 1572, 2426
BseYI CCCAGC 2 cut(s) 334, 1243
Bsh1236I CGCG 2 cut(s) 936, 2764
Bsh1285I CGRYCG 1 cut(s) 1339
BshFI GGCC 5 cut(s) 1195, 1509, 1959, 2220, 2698
BshNI GGYRCC 1 cut(s) 2256
BshTI ACCGGT 1 cut(s) 1334
BsiEI CGRYCG 1 cut(s) 1339
BsiHKAI GWGCWC 3 cut(s) 435, 1302, 2868
BsiHKCI CYCGRG 1 cut(s) 701
BsiSI CCGG 7 cut(s) 252, 339, 1202, 1335, 1510, 2260, 2272
BslI CCNNNNNNNGG 7 cut(s) 560, 707, 779, 1577, 1714, 1790, 2705
BsmAI GTCTC 9 cut(s) 76, 78, 879, 900, 1899, 1923, 2294, 2381, 2872
BsmI GAATGC 2 cut(s) 2282, 2573
BsnI GGCC 5 cut(s) 1195, 1509, 1959, 2220, 2698
Bso31I GGTCTC 4 cut(s) 879, 1923, 2294, 2872
BsoBI CYCGRG 1 cut(s) 701
Bsp119I TTCGAA 2 cut(s) 1877, 2027
Bsp1286I GDGCHC 5 cut(s) 435, 764, 1302, 1371, 2868
Bsp1720I GCTNAGC 4 cut(s) 827, 846, 999, 2919
BspACI CCGC 4 cut(s) 831, 934, 2046, 2157
BspANI GGCC 5 cut(s) 1195, 1509, 1959, 2220, 2698
BspFNI CGCG 2 cut(s) 936, 2764
BspHI TCATGA 2 cut(s) 988, 2209
BspLI GGNNCC 4 cut(s) 835, 1691, 1726, 2258
BspMAI CTGCAG 2 cut(s) 1320, 2230
BspMI ACCTGC 1 cut(s) 2821
BspOI GCTAGC 1 cut(s) 2282
BspPI GGATC 4 cut(s) 125, 976, 1273, 2779
BspQI GCTCTTC 2 cut(s) 2209, 2636
BspT104I TTCGAA 2 cut(s) 1877, 2027
BspT107I GGYRCC 1 cut(s) 2256
BspTNI GGTCTC 4 cut(s) 879, 1923, 2294, 2872
BsrBI CCGCTC 1 cut(s) 2157
BsrDI GCAATG 2 cut(s) 2601, 2832
BsrFI RCCGGY 2 cut(s) 1334, 1509
BsrI ACTGG 7 cut(s) 413, 783, 1051, 1123, 1489, 2560, 2699
BssAI RCCGGY 2 cut(s) 1334, 1509
BssECI CCNNGG 4 cut(s) 77, 356, 1927, 2814
BssT1I CCWWGG 4 cut(s) 77, 356, 1927, 2814
Bst4CI ACNGT 3 cut(s) 749, 1220, 2870
Bst6I CTCTTC 8 cut(s) 30, 1727, 1855, 1879, 2209, 2357, 2636, 2712
BstAPI GCANNNNNTGC 1 cut(s) 1991
BstBI TTCGAA 2 cut(s) 1877, 2027
BstC8I GCNNGC 8 cut(s) 938, 1302, 1451, 2162, 2280, 2535, 2587, 2910
BstEII GGTNACC 1 cut(s) 1416
BstENI CCTNNNNNAGG 1 cut(s) 1712
BstF5I GGATG 4 cut(s) 546, 1210, 1563, 2476
BstFNI CGCG 2 cut(s) 936, 2764
BstH2I RGCGCY 1 cut(s) 514
BstHHI GCGC 2 cut(s) 513, 2766
BstMAI GTCTC 9 cut(s) 76, 78, 879, 900, 1899, 1923, 2294, 2381, 2872
BstMCI CGRYCG 1 cut(s) 1339
BstMWI GCNNNNNNNGC 9 cut(s) 358, 430, 439, 759, 1375, 1384, 1991, 2411, 2519
BstNSI RCATGY 3 cut(s) 2329, 2575, 2944
BstPI GGTNACC 1 cut(s) 1416
BstSFI CTRYAG 2 cut(s) 1316, 2226
BstSLI GKGCMC 1 cut(s) 1371
BstUI CGCG 2 cut(s) 936, 2764
BstV1I GCAGC 3 cut(s) 1302, 1572, 2426
BstV2I GAAGAC 1 cut(s) 4
BstX2I RGATCY 4 cut(s) 130, 981, 1161, 2784
BstXI CCANNNNNNTGG 1 cut(s) 1628
BstYI RGATCY 4 cut(s) 130, 981, 1161, 2784
Bsu36I CCTNAGG 1 cut(s) 1713
BsuRI GGCC 5 cut(s) 1195, 1509, 1959, 2220, 2698
BtgZI GCGATG 1 cut(s) 2661
BtsCI GGATG 4 cut(s) 546, 1210, 1563, 2476
BtsI GCAGTG 1 cut(s) 2853
BtsIMutI CAGTG 6 cut(s) 1044, 1116, 1216, 2553, 2853, 2875
BveI ACCTGC 1 cut(s) 2821
Cac8I GCNNGC 8 cut(s) 938, 1302, 1451, 2162, 2280, 2535, 2587, 2910
CciI TCATGA 2 cut(s) 988, 2209
CfoI GCGC 2 cut(s) 513, 2766
Cfr10I RCCGGY 2 cut(s) 1334, 1509
Cfr13I GGNCC 3 cut(s) 1193, 1631, 1725
Csp6I GTAC 5 cut(s) 1587, 1781, 2323, 2669, 2750
CspAI ACCGGT 1 cut(s) 1334
CviQI GTAC 5 cut(s) 1587, 1781, 2323, 2669, 2750
DraIII CACNNNGTG 1 cut(s) 2872
DrdI GACNNNNNNGTC 2 cut(s) 914, 1853
DseDI GACNNNNNNGTC 2 cut(s) 914, 1853
Eam1104I CTCTTC 8 cut(s) 30, 1727, 1855, 1879, 2209, 2357, 2636, 2712
EarI CTCTTC 8 cut(s) 30, 1727, 1855, 1879, 2209, 2357, 2636, 2712
Ecl136II GAGCTC 2 cut(s) 433, 1300
Eco130I CCWWGG 4 cut(s) 77, 356, 1927, 2814
Eco147I AGGCCT 1 cut(s) 1959
Eco24I GRGCYC 3 cut(s) 435, 764, 1302
Eco31I GGTCTC 4 cut(s) 879, 1923, 2294, 2872
Eco32I GATATC 1 cut(s) 2375
Eco47I GGWCC 2 cut(s) 1631, 1725
Eco47III AGCGCT 1 cut(s) 512
Eco53kI GAGCTC 2 cut(s) 433, 1300
Eco57I CTGAAG 4 cut(s) 527, 809, 1178, 1836
Eco81I CCTNAGG 1 cut(s) 1713
Eco88I CYCGRG 1 cut(s) 701
Eco91I GGTNACC 1 cut(s) 1416
EcoICRI GAGCTC 2 cut(s) 433, 1300
EcoNI CCTNNNNNAGG 1 cut(s) 1712
EcoO109I RGGNCCY 1 cut(s) 1725
EcoO65I GGTNACC 1 cut(s) 1416
EcoRI GAATTC 2 cut(s) 342, 2023
EcoRV GATATC 1 cut(s) 2375
EcoT14I CCWWGG 4 cut(s) 77, 356, 1927, 2814
EcoT22I ATGCAT 2 cut(s) 2573, 2620
EcoT38I GRGCYC 3 cut(s) 435, 764, 1302
ErhI CCWWGG 4 cut(s) 77, 356, 1927, 2814
FalI AAGNNNNNCTT 1 cut(s) 28
FauNDI CATATG 1 cut(s) 2593
FbaI TGATCA 2 cut(s) 2682, 2778
FblI GTMKAC 2 cut(s) 1103, 1339
Fnu4HI GCNGC 4 cut(s) 1316, 1561, 2158, 2415
FokI GGATG 4 cut(s) 533, 1217, 1550, 2483
FriOI GRGCYC 3 cut(s) 435, 764, 1302
Fsp4HI GCNGC 4 cut(s) 1316, 1561, 2158, 2415
GlaI GCGC 2 cut(s) 512, 2765
GluI GCNGC 4 cut(s) 1316, 1561, 2158, 2415
GsaI CCCAGC 2 cut(s) 338, 1247
GsuI CTGGAG 3 cut(s) 1068, 1506, 2592
HaeII RGCGCY 1 cut(s) 514
HaeIII GGCC 5 cut(s) 1195, 1509, 1959, 2220, 2698
HapII CCGG 7 cut(s) 252, 339, 1202, 1335, 1510, 2260, 2272
HhaI GCGC 2 cut(s) 513, 2766
Hin6I GCGC 2 cut(s) 511, 2764
HinP1I GCGC 2 cut(s) 511, 2764
HincII GTYRAC 1 cut(s) 1340
HindII GTYRAC 1 cut(s) 1340
HindIII AAGCTT 6 cut(s) 87, 350, 400, 1064, 1376, 1940
HinfI GANTC 8 cut(s) 693, 1390, 1456, 1735, 1847, 1892, 2458, 2759
HpaII CCGG 7 cut(s) 252, 339, 1202, 1335, 1510, 2260, 2272
HphI GGTGA 6 cut(s) 1410, 1875, 2026, 2035, 2357, 2788
Hpy166II GTNNAC 5 cut(s) 62, 257, 473, 1104, 1340
Hpy8I GTNNAC 5 cut(s) 62, 257, 473, 1104, 1340
Hpy99I CGWCG 1 cut(s) 920
HpyAV CCTTC 5 cut(s) 144, 1068, 1143, 2059, 2765
HpyCH4III ACNGT 3 cut(s) 749, 1220, 2870
HpyCH4IV ACGT 2 cut(s) 1598, 1680
HpyF10VI GCNNNNNNNGC 9 cut(s) 358, 430, 439, 759, 1375, 1384, 1991, 2411, 2519
HpySE526I ACGT 2 cut(s) 1598, 1680
HspAI GCGC 2 cut(s) 511, 2764
Ksp22I TGATCA 2 cut(s) 2682, 2778
LguI GCTCTTC 2 cut(s) 2209, 2636
LmnI GCTCC 5 cut(s) 430, 1049, 1312, 2337, 2611
Lsp1109I GCAGC 3 cut(s) 1302, 1572, 2426
LweI GCATC 6 cut(s) 436, 555, 1436, 1572, 2100, 2626
MaeII ACGT 2 cut(s) 1598, 1680
MaeIII GTNAC 9 cut(s) 197, 487, 570, 1214, 1220, 1416, 1844, 2429, 2597
MbiI CCGCTC 1 cut(s) 2157
MfeI CAATTG 1 cut(s) 2580
MflI RGATCY 4 cut(s) 130, 981, 1161, 2784
MhlI GDGCHC 5 cut(s) 435, 764, 1302, 1371, 2868
MlyI GAGTC 4 cut(s) 1744, 1841, 1901, 2753
MmeI TCCRAC 3 cut(s) 405, 1512, 2505
Mph1103I ATGCAT 2 cut(s) 2573, 2620
MroXI GAANNNNTTC 4 cut(s) 956, 1441, 1673, 2898
MseI TTAA 5 cut(s) 1697, 1809, 2193, 2397, 2475
MslI CAYNNNNRTG 3 cut(s) 2214, 2469, 2566
MspA1I CMGCKG 4 cut(s) 577, 658, 831, 1387
MspI CCGG 7 cut(s) 252, 339, 1202, 1335, 1510, 2260, 2272
MunI CAATTG 1 cut(s) 2580
Mva1269I GAATGC 2 cut(s) 2282, 2573
MvnI CGCG 2 cut(s) 936, 2764
MwoI GCNNNNNNNGC 9 cut(s) 358, 430, 439, 759, 1375, 1384, 1991, 2411, 2519
NdeI CATATG 1 cut(s) 2593
NheI GCTAGC 1 cut(s) 2278
NlaIV GGNNCC 4 cut(s) 835, 1691, 1726, 2258
NmuCI GTSAC 7 cut(s) 197, 487, 570, 1214, 1220, 1416, 1844
NsiI ATGCAT 2 cut(s) 2573, 2620
NspI RCATGY 3 cut(s) 2329, 2575, 2944
NspV TTCGAA 2 cut(s) 1877, 2027
PagI TCATGA 2 cut(s) 988, 2209
PaqCI CACCTGC 1 cut(s) 2821
PceI AGGCCT 1 cut(s) 1959
PciSI GCTCTTC 2 cut(s) 2209, 2636
PctI GAATGC 2 cut(s) 2282, 2573
PdmI GAANNNNTTC 4 cut(s) 956, 1441, 1673, 2898
PfeI GAWTC 4 cut(s) 693, 1390, 1456, 2458
PinAI ACCGGT 1 cut(s) 1334
PkrI GCNGC 4 cut(s) 1317, 1562, 2159, 2416
PleI GAGTC 4 cut(s) 1743, 1841, 1900, 2753
PpsI GAGTC 4 cut(s) 1743, 1841, 1900, 2753
PpuMI RGGWCCY 1 cut(s) 1725
Psp124BI GAGCTC 2 cut(s) 435, 1302
Psp5II RGGWCCY 1 cut(s) 1725
PspEI GGTNACC 1 cut(s) 1416
PspFI CCCAGC 2 cut(s) 334, 1243
PspN4I GGNNCC 4 cut(s) 835, 1691, 1726, 2258
PspPI GGNCC 3 cut(s) 1193, 1631, 1725
PspPPI RGGWCCY 1 cut(s) 1725
PstI CTGCAG 2 cut(s) 1320, 2230
PsuI RGATCY 4 cut(s) 130, 981, 1161, 2784
PvuII CAGCTG 3 cut(s) 577, 658, 1387
RsaI GTAC 5 cut(s) 1588, 1782, 2324, 2670, 2751
RsaNI GTAC 5 cut(s) 1587, 1781, 2323, 2669, 2750
RseI CAYNNNNRTG 3 cut(s) 2214, 2469, 2566
SacI GAGCTC 2 cut(s) 435, 1302
SalI GTCGAC 1 cut(s) 1338
SapI GCTCTTC 2 cut(s) 2209, 2636
SaqAI TTAA 5 cut(s) 1697, 1809, 2193, 2397, 2475
SatI GCNGC 4 cut(s) 1316, 1561, 2158, 2415
Sau96I GGNCC 3 cut(s) 1193, 1631, 1725
SchI GAGTC 4 cut(s) 1744, 1841, 1901, 2753
SduI GDGCHC 5 cut(s) 435, 764, 1302, 1371, 2868
SfaNI GCATC 6 cut(s) 436, 555, 1436, 1572, 2100, 2626
SfcI CTRYAG 2 cut(s) 1316, 2226
SfuI TTCGAA 2 cut(s) 1877, 2027
SinI GGWCC 2 cut(s) 1631, 1725
SmiMI CAYNNNNRTG 3 cut(s) 2214, 2469, 2566
SmlI CTYRAG 6 cut(s) 107, 868, 1396, 1526, 1644, 1920
SmoI CTYRAG 6 cut(s) 107, 868, 1396, 1526, 1644, 1920
SseBI AGGCCT 1 cut(s) 1959
SsiI CCGC 4 cut(s) 831, 934, 2046, 2157
SstI GAGCTC 2 cut(s) 435, 1302
StuI AGGCCT 1 cut(s) 1959
StyI CCWWGG 4 cut(s) 77, 356, 1927, 2814
TaaI ACNGT 3 cut(s) 749, 1220, 2870
TaiI ACGT 2 cut(s) 1601, 1683
TaqI TCGA 7 cut(s) 444, 684, 918, 1339, 1877, 2027, 2508
TatI WGTACW 1 cut(s) 2322
TauI GCSGC 1 cut(s) 2160
TfiI GAWTC 4 cut(s) 693, 1390, 1456, 2458
Tru1I TTAA 5 cut(s) 1697, 1809, 2193, 2397, 2475
Tru9I TTAA 5 cut(s) 1697, 1809, 2193, 2397, 2475
TscAI CASTG 6 cut(s) 1051, 1123, 1223, 2560, 2853, 2875
TseFI GTSAC 7 cut(s) 197, 487, 570, 1214, 1220, 1416, 1844
TseI GCWGC 3 cut(s) 1315, 1560, 2414
Tsp45I GTSAC 7 cut(s) 197, 487, 570, 1214, 1220, 1416, 1844
TspDTI ATGAA 7 cut(s) 17, 28, 607, 949, 1005, 1962, 2453
TspGWI ACGGA 2 cut(s) 38, 990
TspRI CASTG 6 cut(s) 1051, 1123, 1223, 2560, 2853, 2875
VpaK11BI GGWCC 2 cut(s) 1631, 1725
XagI CCTNNNNNAGG 1 cut(s) 1712
XapI RAATTY 9 cut(s) 342, 413, 856, 1491, 1656, 1934, 2023, 2063, 2232
XbaI TCTAGA 1 cut(s) 1603
XceI RCATGY 3 cut(s) 2329, 2575, 2944
XmaJI CCTAGG 1 cut(s) 77
XmiI GTMKAC 2 cut(s) 1103, 1339
XmnI GAANNNNTTC 4 cut(s) 956, 1441, 1673, 2898
Zsp2I ATGCAT 2 cut(s) 2573, 2620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.