AT4G20860

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0017115
Physical Location & Seq
Forward (+)
11172622 .. 11174467
1846 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G20860.1

Sequence Viewer

Length: 1593 bp
ATGAGAGAGCTTTTTATGTATCTATTTCTTCTTTTCTTAGTTCTATGTGTCAAATCTGTTTATTCTACTCCGACAAGAGAACAATTCCAAAATTGCTTGTCCACAAAACAATTCAATTCTACCCTCAAAAACCCTATAAATCTCACCACTCATACACTAGATTCCCGAGTTCACACCGACTTTTCCGAGTCTTCATCTCCAAATTCTAGTTTCCTCAACTTGAATTTCACAAGCTTGAAGCCGATTCTCATCGTTAAGCCAAAATCCGAATCCGAAATAAAACAATCGATTTTATGCAGCAGGAAACTTGGAGTGCAAGTAAGAACAATGAGTGGTGGTCATGACTACGAAGGTCTATCTTACCTCTCACTATCACCTTTCATAATCGTCGACCTCGTTAACCTCAGATCAATCAGCATCAACCTCACAGACGAGACCGCTTGGATCCAATCGGGCGCAACACTCGGCGAAGTCTACTACAAGATCGCCAAAACCAGCAAGATCCATGCCTTTGCCGCAGGGATATGTCCAAGTGTTGGTGTTGGTGGACATATTAGCGGAGGAGGATTCGGTACAATAATGAGGAAATACGGTTTAGCGTCTGATAACGTTGTGGACGCACGTTTGATGGATGTAAATGGGAAAACTCTTGACCGGAAAACGATGGGAGAGGATTTGTTTTGGGCGCTTAGAGGCGGTGGAGCTGCGAGTTTTGGCGTTGTCTTGTCGTGGAAGGTTAAGCTTGCTAGGGTTCCTGAAAAGGTAACTTGTTTCATAAGTCAACATCCGATGGGACCTAGCATGAACAAGCTTGTTCATAGATGGCAATCCATAGGATCAGAGCTAGACGAAGATTTATTCATCAGAGTCATTATTGACAACAGTCTAGAAGGGAATCAAAGAAAAGTGAAATCAACATTTCAAACTCTATTTCTTGGTGGAATCGATAGACTGATTCCTCTGATGAACCAGAAGTTTCCGGAACTCGGCTTACGATCTCAAGACTGTTCGGAAATGAGCTGGATCGAATCGATAATGTTCTTCAACTGGAGATCAGGACAGCCGTTAGAGATTTTGCTCAACAGAGACCTAAGATTCGAGGATCAGTATTTCAAAGCAAAGTCAGATTATGTTCAAAAACCCGTTCCTGAAAACGTTTTCGAAGAAGTAACCAAGAGATTTCTCGAGCAAGATACTCCACTGATGATCTTTGAGCCATTGGGTGGGAAAATCAGCAAGATTTCAGAAACAGAATCTCCATATCCACACAGAAGAGGTAATCTGTATAATATACAGTACATGGTGAAATGGAAAGTGAATGAAGTCGAGGAGATGAACAAACATGTCAGGTGGATGAGATCGTTACACGATTACATGACTCCGTATGTTTCTAAATCGCCGAGAGGAGCTTATTTGAATTACAGAGATCTTGATTTGGGCTCGACCAAAGGGATTAACACGAGTTTCGAAGATGCAAGGAAATGGGGTGAGACTTATTTCAAAGGTAATTTCAAGAGATTAGGTTTGGTTAAAGGGAAGATTGATCCAACAAATTTCTTCAGGAACGAACAGAGTATTCCTCCTCTGTTTTGA

Protein Analysis

530

Amino Acids

60.41

Weight (kDa)

9.14

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 81 - 217 8.1e-23 FAD binding domain
BBE PF08031 470 - 527 3.3e-20 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20860
fragaria_vesca FvH4_3g10830
malus_domestica MD10G1247300.v1.1
prunus_persica Prupe.4G096000_v2.0.a1 Prupe.4G096100_v2.0.a1
pyrus_communis pycom05g25170 pycom10g20520
rosa_chinensis RchiOBHm_Chr5g0017541
rosa_laevigata RLG00000032297
rosa_multiflora Rmu_sc0002565.1_g000003
rosa_roxburghii Rroxscaffold_1G00059900
rosa_rugosa Rorug05G0036800
rosa_wichuraiana Rw5G011300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 536, 1223
AccI GTMKAC 2 cut(s) 390, 474
AccIII TCCGGA 1 cut(s) 979
AciI CCGC 4 cut(s) 438, 516, 558, 696
AclI AACGTT 2 cut(s) 609, 1155
AclWI GGATC 7 cut(s) 439, 452, 496, 844, 1031, 1110, 1538
AcsI RAATTY 3 cut(s) 202, 223, 1552
AcuI CTGAAG 1 cut(s) 1543
AfaI GTAC 2 cut(s) 574, 1298
AfiI CCNNNNNNNGG 3 cut(s) 536, 986, 1223
AflIII ACRYGT 1 cut(s) 1342
AluBI AGCT 8 cut(s) 10, 234, 704, 742, 811, 844, 1020, 1409
AluI AGCT 8 cut(s) 10, 234, 704, 742, 811, 844, 1020, 1409
Alw26I GTCTC 3 cut(s) 428, 1080, 1484
AlwI GGATC 7 cut(s) 439, 452, 496, 844, 1031, 1110, 1538
Ama87I CYCGRG 2 cut(s) 165, 1184
Aor13HI TCCGGA 1 cut(s) 979
ApeKI GCWGC 2 cut(s) 297, 704
ApoI RAATTY 3 cut(s) 202, 223, 1552
ArsI GACNNNNNNTTYG 2 cut(s) 1079, 1111
AspLEI GCGC 2 cut(s) 458, 688
AspS9I GGNCC 1 cut(s) 794
AsuHPI GGTGA 4 cut(s) 136, 366, 1315, 1499
AsuII TTCGAA 2 cut(s) 1161, 1467
AvaI CYCGRG 2 cut(s) 165, 1184
AvaII GGWCC 1 cut(s) 794
BamHI GGATCC 1 cut(s) 444
BanII GRGCYC 1 cut(s) 1442
BauI CACGAG 1 cut(s) 1459
BbsI GAAGAC 1 cut(s) 183
BbvI GCAGC 2 cut(s) 309, 691
BccI CCATC 4 cut(s) 622, 658, 784, 816
BceAI ACGGC 1 cut(s) 1048
BcoDI GTCTC 3 cut(s) 428, 1080, 1484
BfaI CTAG 6 cut(s) 158, 207, 747, 798, 845, 887
BfoI RGCGCY 1 cut(s) 689
BglII AGATCT 1 cut(s) 1426
BisI GCNGC 3 cut(s) 298, 516, 705
BlsI GCNGC 3 cut(s) 299, 517, 706
Bme18I GGWCC 1 cut(s) 794
BmeT110I CYCGRG 2 cut(s) 165, 1184
BmgT120I GGNCC 1 cut(s) 794
BmiI GGNNCC 3 cut(s) 446, 753, 795
BmsI GCATC 2 cut(s) 426, 1462
BoxI GACNNNNGTC 1 cut(s) 882
BpiI GAAGAC 1 cut(s) 183
BplI GAGNNNNNCTC 1 cut(s) 1564
BpmI CTGGAG 1 cut(s) 1069
Bpu14I TTCGAA 2 cut(s) 1161, 1467
BpuEI CTTGAG 1 cut(s) 984
Bsa29I ATCGAT 3 cut(s) 287, 945, 1031
BsaBI GATNNNNATC 2 cut(s) 248, 826
BsaI GGTCTC 2 cut(s) 428, 1080
BsaWI WCCGGW 2 cut(s) 654, 979
BsaXI ACNNNNNCTCC 4 cut(s) 303, 333, 1240, 1270
Bsc4I CCNNNNNNNGG 3 cut(s) 536, 986, 1223
Bse1I ACTGG 1 cut(s) 1052
Bse8I GATNNNNATC 2 cut(s) 248, 826
BseAI TCCGGA 1 cut(s) 979
BseCI ATCGAT 3 cut(s) 287, 945, 1031
BseGI GGATG 3 cut(s) 637, 784, 1359
BseJI GATNNNNATC 2 cut(s) 248, 826
BseLI CCNNNNNNNGG 3 cut(s) 536, 986, 1223
BseMII CTCAG 1 cut(s) 418
BseNI ACTGG 1 cut(s) 1052
BseRI GAGGAG 4 cut(s) 576, 1343, 1419, 1572
BseXI GCAGC 2 cut(s) 309, 691
BshVI ATCGAT 3 cut(s) 287, 945, 1031
BsiHKCI CYCGRG 2 cut(s) 165, 1184
BsiSI CCGG 2 cut(s) 655, 980
BslFI GGGAC 1 cut(s) 807
BslI CCNNNNNNNGG 3 cut(s) 536, 986, 1223
BsmAI GTCTC 3 cut(s) 428, 1080, 1484
BsmFI GGGAC 1 cut(s) 807
Bso31I GGTCTC 2 cut(s) 428, 1080
BsoBI CYCGRG 2 cut(s) 165, 1184
Bsp119I TTCGAA 2 cut(s) 1161, 1467
Bsp1286I GDGCHC 1 cut(s) 1442
Bsp13I TCCGGA 1 cut(s) 979
BspACI CCGC 4 cut(s) 438, 516, 558, 696
BspCNI CTCAG 1 cut(s) 417
BspDI ATCGAT 3 cut(s) 287, 945, 1031
BspEI TCCGGA 1 cut(s) 979
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 3 cut(s) 446, 753, 795
BspPI GGATC 7 cut(s) 439, 452, 496, 844, 1031, 1110, 1538
BspT104I TTCGAA 2 cut(s) 1161, 1467
BspTNI GGTCTC 2 cut(s) 428, 1080
BsrI ACTGG 1 cut(s) 1052
BssSI CACGAG 1 cut(s) 1459
Bst2BI CACGAG 1 cut(s) 1459
Bst4CI ACNGT 4 cut(s) 593, 884, 1007, 1296
Bst6I CTCTTC 1 cut(s) 1267
BstBI TTCGAA 2 cut(s) 1161, 1467
BstC8I GCNNGC 1 cut(s) 744
BstDEI CTNAG 4 cut(s) 37, 404, 689, 1091
BstF5I GGATG 3 cut(s) 637, 784, 1359
BstH2I RGCGCY 1 cut(s) 689
BstHHI GCGC 2 cut(s) 458, 688
BstMAI GTCTC 3 cut(s) 428, 1080, 1484
BstMWI GCNNNNNNNGC 1 cut(s) 515
BstNSI RCATGY 1 cut(s) 1346
BstPAI GACNNNNGTC 1 cut(s) 882
BstV1I GCAGC 2 cut(s) 309, 691
BstV2I GAAGAC 1 cut(s) 183
BstX2I RGATCY 3 cut(s) 444, 501, 1426
BstYI RGATCY 3 cut(s) 444, 501, 1426
Bsu15I ATCGAT 3 cut(s) 287, 945, 1031
BsuTUI ATCGAT 3 cut(s) 287, 945, 1031
BtsCI GGATG 3 cut(s) 637, 784, 1359
BtsIMutI CAGTG 1 cut(s) 1199
Cac8I GCNNGC 1 cut(s) 744
CciI TCATGA 1 cut(s) 340
CfoI GCGC 2 cut(s) 458, 688
Cfr13I GGNCC 1 cut(s) 794
ClaI ATCGAT 3 cut(s) 287, 945, 1031
CseI GACGC 2 cut(s) 588, 626
Csp6I GTAC 2 cut(s) 573, 1297
CviAII CATG 6 cut(s) 341, 506, 802, 1300, 1343, 1375
CviQI GTAC 2 cut(s) 573, 1297
DdeI CTNAG 4 cut(s) 37, 404, 689, 1091
Eam1104I CTCTTC 1 cut(s) 1267
EarI CTCTTC 1 cut(s) 1267
Eco24I GRGCYC 1 cut(s) 1442
Eco31I GGTCTC 2 cut(s) 428, 1080
Eco47I GGWCC 1 cut(s) 794
Eco57I CTGAAG 1 cut(s) 1543
Eco88I CYCGRG 2 cut(s) 165, 1184
EcoO109I RGGNCCY 1 cut(s) 794
EcoT38I GRGCYC 1 cut(s) 1442
FaeI CATG 6 cut(s) 344, 509, 805, 1303, 1346, 1378
FaqI GGGAC 1 cut(s) 807
FatI CATG 6 cut(s) 340, 505, 801, 1299, 1342, 1374
FblI GTMKAC 2 cut(s) 390, 474
Fnu4HI GCNGC 3 cut(s) 298, 516, 705
FokI GGATG 3 cut(s) 644, 771, 1366
FriOI GRGCYC 1 cut(s) 1442
Fsp4HI GCNGC 3 cut(s) 298, 516, 705
FspBI CTAG 6 cut(s) 158, 207, 747, 798, 845, 887
GlaI GCGC 2 cut(s) 457, 687
GluI GCNGC 3 cut(s) 298, 516, 705
GsuI CTGGAG 1 cut(s) 1069
HaeII RGCGCY 1 cut(s) 689
HapII CCGG 2 cut(s) 655, 980
HgaI GACGC 2 cut(s) 588, 626
HhaI GCGC 2 cut(s) 458, 688
Hin1II CATG 6 cut(s) 344, 509, 805, 1303, 1346, 1378
Hin6I GCGC 2 cut(s) 456, 686
HinP1I GCGC 2 cut(s) 456, 686
HincII GTYRAC 3 cut(s) 391, 400, 782
HindII GTYRAC 3 cut(s) 391, 400, 782
HindIII AAGCTT 3 cut(s) 232, 740, 809
HpaI GTTAAC 1 cut(s) 400
HpaII CCGG 2 cut(s) 655, 980
HphI GGTGA 4 cut(s) 136, 366, 1315, 1499
Hpy166II GTNNAC 8 cut(s) 102, 172, 391, 400, 475, 548, 616, 782
Hpy8I GTNNAC 8 cut(s) 102, 172, 391, 400, 475, 548, 616, 782
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 3 cut(s) 344, 727, 884
HpyCH4III ACNGT 4 cut(s) 593, 884, 1007, 1296
HpyCH4IV ACGT 3 cut(s) 609, 622, 1155
HpyCH4V TGCA 3 cut(s) 297, 316, 1475
HpyF10VI GCNNNNNNNGC 1 cut(s) 515
HpyF3I CTNAG 4 cut(s) 37, 404, 689, 1091
HpySE526I ACGT 3 cut(s) 609, 622, 1155
Hsp92II CATG 6 cut(s) 344, 509, 805, 1303, 1346, 1378
HspAI GCGC 2 cut(s) 456, 686
Kpn2I TCCGGA 1 cut(s) 979
KspAI GTTAAC 1 cut(s) 400
LmnI GCTCC 2 cut(s) 701, 1406
Lsp1109I GCAGC 2 cut(s) 309, 691
LweI GCATC 2 cut(s) 426, 1462
MaeI CTAG 6 cut(s) 158, 207, 747, 798, 845, 887
MaeII ACGT 3 cut(s) 609, 622, 1155
MaeIII GTNAC 3 cut(s) 763, 1168, 1362
MboII GAAGA 9 cut(s) 20, 183, 863, 1033, 1175, 1284, 1481, 1549, 1549
MflI RGATCY 3 cut(s) 444, 501, 1426
MhlI GDGCHC 1 cut(s) 1442
MluCI AATT 9 cut(s) 83, 91, 110, 115, 202, 223, 1417, 1507, 1552
MlyI GAGTC 3 cut(s) 197, 876, 1372
MmeI TCCRAC 2 cut(s) 95, 1571
MroI TCCGGA 1 cut(s) 979
MseI TTAA 5 cut(s) 255, 399, 738, 1455, 1530
MspI CCGG 2 cut(s) 655, 980
MwoI GCNNNNNNNGC 1 cut(s) 515
NlaIII CATG 6 cut(s) 344, 509, 805, 1303, 1346, 1378
NlaIV GGNNCC 3 cut(s) 446, 753, 795
NmeAIII GCCGAG 3 cut(s) 444, 966, 1425
NspI RCATGY 1 cut(s) 1346
NspV TTCGAA 2 cut(s) 1161, 1467
PaeR7I CTCGAG 1 cut(s) 1184
PagI TCATGA 1 cut(s) 340
PciI ACATGT 1 cut(s) 1342
PcsI WCGNNNNNNNCGW 1 cut(s) 393
PflMI CCANNNNNTGG 2 cut(s) 536, 1223
PkrI GCNGC 3 cut(s) 299, 517, 706
PleI GAGTC 3 cut(s) 196, 875, 1372
PpsI GAGTC 3 cut(s) 196, 875, 1372
PpuMI RGGWCCY 1 cut(s) 794
PscI ACATGT 1 cut(s) 1342
PshAI GACNNNNGTC 1 cut(s) 882
Psp1406I AACGTT 2 cut(s) 609, 1155
Psp5II RGGWCCY 1 cut(s) 794
PspN4I GGNNCC 3 cut(s) 446, 753, 795
PspPI GGNCC 1 cut(s) 794
PspPPI RGGWCCY 1 cut(s) 794
PsrI GAACNNNNNNTAC 2 cut(s) 975, 1007
PsuI RGATCY 3 cut(s) 444, 501, 1426
RsaI GTAC 2 cut(s) 574, 1298
RsaNI GTAC 2 cut(s) 573, 1297
SalI GTCGAC 1 cut(s) 389
SaqAI TTAA 5 cut(s) 255, 399, 738, 1455, 1530
SatI GCNGC 3 cut(s) 298, 516, 705
Sau96I GGNCC 1 cut(s) 794
SchI GAGTC 3 cut(s) 197, 876, 1372
SduI GDGCHC 1 cut(s) 1442
SfaNI GCATC 2 cut(s) 426, 1462
Sfr274I CTCGAG 1 cut(s) 1184
SfuI TTCGAA 2 cut(s) 1161, 1467
SinI GGWCC 1 cut(s) 794
SlaI CTCGAG 1 cut(s) 1184
SmlI CTYRAG 2 cut(s) 999, 1184
SmoI CTYRAG 2 cut(s) 999, 1184
Sse9I AATT 9 cut(s) 83, 91, 110, 115, 202, 223, 1417, 1507, 1552
SsiI CCGC 4 cut(s) 438, 516, 558, 696
SspMI CTAG 6 cut(s) 158, 207, 747, 798, 845, 887
TaaI ACNGT 4 cut(s) 593, 884, 1007, 1296
TaiI ACGT 3 cut(s) 612, 625, 1158
TasI AATT 9 cut(s) 83, 91, 110, 115, 202, 223, 1417, 1507, 1552
TatI WGTACW 1 cut(s) 1296
TauI GCSGC 1 cut(s) 518
Tru1I TTAA 5 cut(s) 255, 399, 738, 1455, 1530
Tru9I TTAA 5 cut(s) 255, 399, 738, 1455, 1530
TscAI CASTG 1 cut(s) 1206
TseI GCWGC 2 cut(s) 297, 704
TspDTI ATGAA 9 cut(s) 183, 370, 763, 806, 818, 850, 980, 1335, 1349
TspGWI ACGGA 1 cut(s) 1371
TspRI CASTG 1 cut(s) 1206
Van91I CCANNNNNTGG 2 cut(s) 536, 1223
VpaK11BI GGWCC 1 cut(s) 794
XapI RAATTY 3 cut(s) 202, 223, 1552
XbaI TCTAGA 1 cut(s) 886
XceI RCATGY 1 cut(s) 1346
XhoI CTCGAG 1 cut(s) 1184
XmiI GTMKAC 2 cut(s) 390, 474
XspI CTAG 6 cut(s) 158, 207, 747, 798, 845, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.