Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0017115

Physical Location & Seq

Genomic Coordinates Forward (+)

34045595 .. 34047199

Gene Structure 1605 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1247300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1605 bp

ATGCCTAAATTCCTGAAACAGATGGAGTTCATTGCACTACTAGCTTCTTTGCTCTTGGTCTCAATTCCTTCGGTATCTTCTGTTTCAATCAGTGGTGAAATTTTCAGTCAGTGCATGGCAGACTACAGCAAAATTTCTGATCAAAACATTCACACCTCAAACTCTTCTTTGTATTCATCTCTTTTGCAATCTTCCCAGCAAAACCCTAGGTGGTTAAACTCCACATCGAAGCCTCTTCTCCTTGTCACACCTTCCCATGAGTCTGAAATCCAAGCCGCTGTTCTTTGCAGCAGGAAACTTGGCCTGCAAGTCAGGGTTAGAAGTGGAGGACATGACTATGAAGGCCTATCTTACCTCTGCAAAACCGCATTTATCATCATTGACCTCATCAATCTCCGTTCCCTCAAGGTCAACCTTGCAGATGAAACAGCTTGGGTGCAGTCAGGAGCCACACTTGGTGAGCTTTATTATAGCATTGCCATGAAAAGTGAGGTCCATGGATTTCCAGGAGGTTTGTGCCCTACTGTTGGGATTGGAGGGCACTTCAGTGGAGGTGGGTTTGGAACCCAAATCAGGAAGCATGGCCTAGCAGCTGACAATGTCATAGATGCTCGGTTAATTGATGTGAATGGAAGAATCCTTGACAGAAGAACAATGGGTGAGGATTTGTTTTGGGCCATTAGAGGAGGTGGGGGATCAAGTTTTGGGATCATAGTTTCATGGAAAATCAAATTGGTTCAGGTTCCAAAAATTGTGACGGGTTTTACATTTGACAAAACCCTAGCCGAAGGAGCTACCAGCCTTGTTCACAGGTGGCAATATATTGCAGACAAATTTCATGAAGATCTTTTCATCAGAGTCATAATTCAGAAAGTGGGGAGTGGGAATCAGAAAACGGTTCAAGCATCTTTCCAATCACTGTTTCTTGGTAAAATTGACAGATTAATCCCACTGATTAAGCTGAGTTTTCCAGAGCTGGGATTGCAGGCAAAAGACTGCATTGAGATGAGTTGGATCCAATCTGCCCAGTATTTTGCTGGATTCCAAAAAGACCAGCCCTCAGATGTATTGCTATCTAGAAATCCCCTCTGGAAGATGAATTTCAAGGCTAAATCCGACTTCGTGAAGAACCCAATACCCGAATCGGGACTCCAAGGGATTTGGGAGAGGTTTCAGCAGGAACAAAATGTGTACATGATTTTGGATCCATATGGTGGCAAAATGAATGAGATCTCAGAACATGAAATCCCATTTCCTCACAGAAAAGGTAATTTGTTCAACATACAGTACATAGTGAAGTGGGATGGAAGTGGAGTGGAGGAAACCAACAAGCATATCCACTGGATAAGAATGCTTTACAGATTCATGAGCCCTTATGTTTCTAGGTCCCCAAGGGGTGCCTATATCAATTACAAGGACCTTGATTTGGGTGCCAACGTGCAAAACACAAGCTACTTGGAGGCAAGTACTTGGGGCACAAGGTACTTCAAGGGTAACTTCAAGAGGCTGGCACAGGTGAAGAGCAAGGCCGATCCAGATAACTTCTTCAGGAATGAACAAAGCATCCCTCCTCTTCCTTCTTTGTTTGAAATCACAGAGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0009505 GO:0009719 GO:0009725 GO:0009753 GO:0010033 GO:0030312 GO:0042221 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00940 ko01100 ko01110 map00940 map01100 map01110

Pfam Domains

Protein Families

BBE FAD_binding_4

Protein Analysis

535

Amino Acids

60.0

Weight (kDa)

9.0

Isoelectric Point (pI)

39.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	78 - 214	4.5e-24	FAD binding domain
BBE	PF08031	467 - 523	3e-20	Berberine and berberine like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017115)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20860
fragaria_vesca	FvH4_3g10830
malus_domestica	MD10G1247300.v1.1
prunus_persica	Prupe.4G096000_v2.0.a1 Prupe.4G096100_v2.0.a1
pyrus_communis	pycom05g25170 pycom10g20520
rosa_chinensis	RchiOBHm_Chr5g0017541
rosa_laevigata	RLG00000032297
rosa_multiflora	Rmu_sc0002565.1_g000003
rosa_roxburghii	Rroxscaffold_1G00059900
rosa_rugosa	Rorug05G0036800
rosa_wichuraiana	Rw5G011300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	1397, 1430
AccB7I	CCANNNNNTGG	1 cut(s)	1214
AciI	CCGC	2 cut(s)	276, 366
AclWI	GGATC	7 cut(s)	703, 716, 1009, 1022, 1199, 1212, 1526
AcsI	RAATTY	5 cut(s)	8, 99, 132, 833, 1099
AcuI	CTGAAG	2 cut(s)	529, 1531
AdeI	CACNNNGTG	1 cut(s)	458
AfaI	GTAC	4 cut(s)	1193, 1289, 1468, 1484
AfiI	CCNNNNNNNGG	6 cut(s)	527, 573, 977, 1145, 1214, 1426
AgsI	TTSAA	7 cut(s)	87, 902, 1105, 1279, 1489, 1501, 1589
AjnI	CCWGG	1 cut(s)	505
AjuI	GAANNNNNNNTTGG	6 cut(s)	264, 296, 716, 748, 906, 938
AleI	CACNNNNGTG	1 cut(s)	546
AloI	GAACNNNNNNTCC	4 cut(s)	1230, 1262, 1548, 1580
AluBI	AGCT	8 cut(s)	44, 431, 463, 593, 794, 961, 976, 1452
AluI	AGCT	8 cut(s)	44, 431, 463, 593, 794, 961, 976, 1452
Alw26I	GTCTC	1 cut(s)	64
AlwI	GGATC	7 cut(s)	703, 716, 1009, 1022, 1199, 1212, 1526
AoxI	GGCC	5 cut(s)	301, 343, 583, 675, 1527
ApeKI	GCWGC	2 cut(s)	288, 590
ApoI	RAATTY	5 cut(s)	8, 99, 132, 833, 1099
AseI	ATTAAT	1 cut(s)	944
AspA2I	CCTAGG	1 cut(s)	206
AspS9I	GGNCC	4 cut(s)	493, 675, 1386, 1417
AsuHPI	GGTGA	4 cut(s)	107, 470, 671, 1528
AvaII	GGWCC	3 cut(s)	493, 1386, 1417
AvrII	CCTAGG	1 cut(s)	206
BaeGI	GKGCMC	3 cut(s)	521, 543, 1478
BamHI	GGATCC	2 cut(s)	1014, 1204
BanI	GGYRCC	2 cut(s)	1397, 1430
BanII	GRGCYC	1 cut(s)	1373
BbvI	GCAGC	2 cut(s)	300, 602
BccI	CCATC	2 cut(s)	16, 1298
BciT130I	CCWGG	1 cut(s)	507
BclI	TGATCA	1 cut(s)	139
BcoDI	GTCTC	1 cut(s)	64
BfaI	CTAG	6 cut(s)	41, 207, 587, 782, 1077, 1383
BfmI	CTRYAG	1 cut(s)	124
BglII	AGATCT	2 cut(s)	844, 1230
BisI	GCNGC	3 cut(s)	276, 289, 591
BlnI	CCTAGG	1 cut(s)	206
BlsI	GCNGC	3 cut(s)	277, 290, 592
BmcAI	AGTACT	1 cut(s)	1468
Bme1390I	CCNGG	1 cut(s)	507
Bme18I	GGWCC	3 cut(s)	493, 1386, 1417
BmgT120I	GGNCC	4 cut(s)	493, 675, 1386, 1417
BmiI	GGNNCC	8 cut(s)	448, 565, 744, 1016, 1206, 1388, 1399, 1432
BmrFI	CCNGG	1 cut(s)	507
BmrI	ACTGGG	1 cut(s)	1021
BmsI	GCATC	3 cut(s)	598, 914, 1572
BmuI	ACTGGG	1 cut(s)	1021
BpuEI	CTTGAG	1 cut(s)	389
BsaI	GGTCTC	1 cut(s)	64
BsaJI	CCNNGG	4 cut(s)	206, 496, 1153, 1391
Bsc4I	CCNNNNNNNGG	6 cut(s)	527, 573, 977, 1145, 1214, 1426
Bse1I	ACTGG	2 cut(s)	1027, 1346
Bse3DI	GCAATG	2 cut(s)	30, 474
BseBI	CCWGG	1 cut(s)	507
BseDI	CCNNGG	4 cut(s)	206, 496, 1153, 1391
BseGI	GGATG	2 cut(s)	1309, 1563
BseLI	CCNNNNNNNGG	6 cut(s)	527, 573, 977, 1145, 1214, 1426
BseMI	GCAATG	2 cut(s)	30, 474
BseMII	CTCAG	3 cut(s)	953, 1074, 1248
BseNI	ACTGG	2 cut(s)	1027, 1346
BseRI	GAGGAG	2 cut(s)	699, 1560
BseSI	GKGCMC	3 cut(s)	521, 543, 1478
BseXI	GCAGC	2 cut(s)	300, 602
BseYI	CCCAGC	2 cut(s)	195, 976
BsgI	GTGCAG	1 cut(s)	458
BshFI	GGCC	5 cut(s)	303, 345, 585, 677, 1529
BshNI	GGYRCC	2 cut(s)	1397, 1430
BslFI	GGGAC	2 cut(s)	1161, 1372
BslI	CCNNNNNNNGG	6 cut(s)	527, 573, 977, 1145, 1214, 1426
BsmAI	GTCTC	1 cut(s)	64
BsmFI	GGGAC	2 cut(s)	1161, 1372
BsmI	GAATGC	1 cut(s)	1356
BsnI	GGCC	5 cut(s)	303, 345, 585, 677, 1529
Bso31I	GGTCTC	1 cut(s)	64
Bsp1286I	GDGCHC	4 cut(s)	521, 543, 1373, 1478
Bsp1407I	TGTACA	1 cut(s)	1191
Bsp143I	GATC	8 cut(s)	139, 695, 708, 844, 1014, 1204, 1230, 1531
Bsp19I	CCATGG	1 cut(s)	496
BspACI	CCGC	2 cut(s)	276, 366
BspANI	GGCC	5 cut(s)	303, 345, 585, 677, 1529
BspCNI	CTCAG	3 cut(s)	954, 1073, 1247
BspHI	TCATGA	2 cut(s)	838, 1365
BspLI	GGNNCC	8 cut(s)	448, 565, 744, 1016, 1206, 1388, 1399, 1432
BspPI	GGATC	7 cut(s)	703, 716, 1009, 1022, 1199, 1212, 1526
BspQI	GCTCTTC	1 cut(s)	1514
BspT107I	GGYRCC	2 cut(s)	1397, 1430
BspTNI	GGTCTC	1 cut(s)	64
BsrDI	GCAATG	2 cut(s)	30, 474
BsrGI	TGTACA	1 cut(s)	1191
BsrI	ACTGG	2 cut(s)	1027, 1346
BssECI	CCNNGG	4 cut(s)	206, 496, 1153, 1391
BssMI	GATC	8 cut(s)	139, 695, 708, 844, 1014, 1204, 1230, 1531
BssT1I	CCWWGG	4 cut(s)	206, 496, 1153, 1391
Bst2UI	CCWGG	1 cut(s)	507
Bst4CI	ACNGT	4 cut(s)	526, 898, 921, 1287
Bst6I	CTCTTC	4 cut(s)	169, 240, 1514, 1578
BstAUI	TGTACA	1 cut(s)	1191
BstC8I	GCNNGC	3 cut(s)	305, 987, 1509
BstDEI	CTNAG	3 cut(s)	962, 1060, 1234
BstDSI	CCRYGG	1 cut(s)	496
BstF5I	GGATG	2 cut(s)	1309, 1563
BstKTI	GATC	8 cut(s)	142, 698, 711, 847, 1017, 1207, 1233, 1534
BstMAI	GTCTC	1 cut(s)	64
BstMBI	GATC	8 cut(s)	139, 695, 708, 844, 1014, 1204, 1230, 1531
BstMWI	GCNNNNNNNGC	3 cut(s)	41, 791, 982
BstNI	CCWGG	1 cut(s)	507
BstSCI	CCNGG	1 cut(s)	505
BstSFI	CTRYAG	1 cut(s)	124
BstSLI	GKGCMC	3 cut(s)	521, 543, 1478
BstV1I	GCAGC	2 cut(s)	300, 602
BstX2I	RGATCY	4 cut(s)	844, 1014, 1204, 1230
BstYI	RGATCY	4 cut(s)	844, 1014, 1204, 1230
BsuRI	GGCC	5 cut(s)	303, 345, 585, 677, 1529
BtgI	CCRYGG	1 cut(s)	496
BtsCI	GGATG	2 cut(s)	1309, 1563
BtsIMutI	CAGTG	6 cut(s)	97, 116, 553, 917, 950, 1339
Cac8I	GCNNGC	3 cut(s)	305, 987, 1509
CciI	TCATGA	2 cut(s)	838, 1365
Cfr13I	GGNCC	4 cut(s)	493, 675, 1386, 1417
Csp6I	GTAC	4 cut(s)	1192, 1288, 1467, 1483
CviQI	GTAC	4 cut(s)	1192, 1288, 1467, 1483
DdeI	CTNAG	3 cut(s)	962, 1060, 1234
DpnI	GATC	8 cut(s)	141, 697, 710, 846, 1016, 1206, 1232, 1533
DpnII	GATC	8 cut(s)	139, 695, 708, 844, 1014, 1204, 1230, 1531
DraIII	CACNNNGTG	1 cut(s)	458
Eam1104I	CTCTTC	4 cut(s)	169, 240, 1514, 1578
EarI	CTCTTC	4 cut(s)	169, 240, 1514, 1578
Eco130I	CCWWGG	4 cut(s)	206, 496, 1153, 1391
Eco147I	AGGCCT	1 cut(s)	345
Eco24I	GRGCYC	1 cut(s)	1373
Eco31I	GGTCTC	1 cut(s)	64
Eco47I	GGWCC	3 cut(s)	493, 1386, 1417
Eco57I	CTGAAG	2 cut(s)	529, 1531
EcoO109I	RGGNCCY	2 cut(s)	1386, 1417
EcoRII	CCWGG	1 cut(s)	505
EcoT14I	CCWWGG	4 cut(s)	206, 496, 1153, 1391
EcoT38I	GRGCYC	1 cut(s)	1373
ErhI	CCWWGG	4 cut(s)	206, 496, 1153, 1391
FalI	AAGNNNNNCTT	2 cut(s)	1481, 1513
FaqI	GGGAC	2 cut(s)	1161, 1372
FauNDI	CATATG	1 cut(s)	1210
FbaI	TGATCA	1 cut(s)	139
Fnu4HI	GCNGC	3 cut(s)	276, 289, 591
FokI	GGATG	2 cut(s)	1316, 1550
FriOI	GRGCYC	1 cut(s)	1373
Fsp4HI	GCNGC	3 cut(s)	276, 289, 591
FspBI	CTAG	6 cut(s)	41, 207, 587, 782, 1077, 1383
GluI	GCNGC	3 cut(s)	276, 289, 591
GsaI	CCCAGC	2 cut(s)	199, 980
HaeIII	GGCC	5 cut(s)	303, 345, 585, 677, 1529
HincII	GTYRAC	1 cut(s)	412
HindII	GTYRAC	1 cut(s)	412
HinfI	GANTC	8 cut(s)	260, 636, 858, 886, 1041, 1142, 1149, 1362
HphI	GGTGA	4 cut(s)	107, 470, 671, 1528
Hpy166II	GTNNAC	3 cut(s)	412, 808, 1192
Hpy188I	TCNGA	8 cut(s)	139, 265, 857, 870, 891, 1063, 1117, 1237
Hpy8I	GTNNAC	3 cut(s)	412, 808, 1192
HpyAV	CCTTC	5 cut(s)	78, 261, 335, 782, 1587
HpyCH4III	ACNGT	4 cut(s)	526, 898, 921, 1287
HpyCH4IV	ACGT	1 cut(s)	1437
HpyF10VI	GCNNNNNNNGC	3 cut(s)	41, 791, 982
HpyF3I	CTNAG	3 cut(s)	962, 1060, 1234
HpySE526I	ACGT	1 cut(s)	1437
Ksp22I	TGATCA	1 cut(s)	139
Kzo9I	GATC	8 cut(s)	139, 695, 708, 844, 1014, 1204, 1230, 1531
LguI	GCTCTTC	1 cut(s)	1514
LmnI	GCTCC	2 cut(s)	446, 791
Lsp1109I	GCAGC	2 cut(s)	300, 602
LweI	GCATC	3 cut(s)	598, 914, 1572
MaeI	CTAG	6 cut(s)	41, 207, 587, 782, 1077, 1383
MaeII	ACGT	1 cut(s)	1437
MaeIII	GTNAC	3 cut(s)	244, 754, 1493
MalI	GATC	8 cut(s)	141, 697, 710, 846, 1016, 1206, 1232, 1533
MboI	GATC	8 cut(s)	139, 695, 708, 844, 1014, 1204, 1230, 1531
MflI	RGATCY	4 cut(s)	844, 1014, 1204, 1230
MhlI	GDGCHC	4 cut(s)	521, 543, 1373, 1478
MlyI	GAGTC	3 cut(s)	269, 867, 1143
MmeI	TCCRAC	2 cut(s)	992, 1140
MseI	TTAA	4 cut(s)	215, 617, 944, 957
MslI	CAYNNNNRTG	4 cut(s)	336, 479, 546, 1004
MspA1I	CMGCKG	2 cut(s)	278, 593
MspR9I	CCNGG	1 cut(s)	507
Mva1269I	GAATGC	1 cut(s)	1356
MvaI	CCWGG	1 cut(s)	507
MwoI	GCNNNNNNNGC	3 cut(s)	41, 791, 982
NcoI	CCATGG	1 cut(s)	496
NdeI	CATATG	1 cut(s)	1210
NdeII	GATC	8 cut(s)	139, 695, 708, 844, 1014, 1204, 1230, 1531
NlaIV	GGNNCC	8 cut(s)	448, 565, 744, 1016, 1206, 1388, 1399, 1432
NmuCI	GTSAC	2 cut(s)	244, 754
OliI	CACNNNNGTG	1 cut(s)	546
PagI	TCATGA	2 cut(s)	838, 1365
PceI	AGGCCT	1 cut(s)	345
PciSI	GCTCTTC	1 cut(s)	1514
PctI	GAATGC	1 cut(s)	1356
PfeI	GAWTC	5 cut(s)	636, 886, 1041, 1142, 1362
PflFI	GACNNNGTC	1 cut(s)	599
PflMI	CCANNNNNTGG	1 cut(s)	1214
PfoI	TCCNGGA	1 cut(s)	505
PkrI	GCNGC	3 cut(s)	277, 290, 592
PleI	GAGTC	3 cut(s)	268, 866, 1143
PpsI	GAGTC	3 cut(s)	268, 866, 1143
PpuMI	RGGWCCY	2 cut(s)	1386, 1417
PshBI	ATTAAT	1 cut(s)	944
Psp5II	RGGWCCY	2 cut(s)	1386, 1417
Psp6I	CCWGG	1 cut(s)	505
PspFI	CCCAGC	2 cut(s)	195, 976
PspGI	CCWGG	1 cut(s)	505
PspN4I	GGNNCC	8 cut(s)	448, 565, 744, 1016, 1206, 1388, 1399, 1432
PspPI	GGNCC	4 cut(s)	493, 675, 1386, 1417
PspPPI	RGGWCCY	2 cut(s)	1386, 1417
PsuI	RGATCY	4 cut(s)	844, 1014, 1204, 1230
PsyI	GACNNNGTC	1 cut(s)	599
PvuII	CAGCTG	1 cut(s)	593
RsaI	GTAC	4 cut(s)	1193, 1289, 1468, 1484
RsaNI	GTAC	4 cut(s)	1192, 1288, 1467, 1483
RseI	CAYNNNNRTG	4 cut(s)	336, 479, 546, 1004
SapI	GCTCTTC	1 cut(s)	1514
SaqAI	TTAA	4 cut(s)	215, 617, 944, 957
SatI	GCNGC	3 cut(s)	276, 289, 591
Sau3AI	GATC	8 cut(s)	139, 695, 708, 844, 1014, 1204, 1230, 1531
Sau96I	GGNCC	4 cut(s)	493, 675, 1386, 1417
ScaI	AGTACT	1 cut(s)	1468
SchI	GAGTC	3 cut(s)	269, 867, 1143
ScrFI	CCNGG	1 cut(s)	507
SduI	GDGCHC	4 cut(s)	521, 543, 1373, 1478
SfaNI	GCATC	3 cut(s)	598, 914, 1572
SfcI	CTRYAG	1 cut(s)	124
SinI	GGWCC	3 cut(s)	493, 1386, 1417
SmiMI	CAYNNNNRTG	4 cut(s)	336, 479, 546, 1004
SmlI	CTYRAG	1 cut(s)	404
SmoI	CTYRAG	1 cut(s)	404
SseBI	AGGCCT	1 cut(s)	345
SsiI	CCGC	2 cut(s)	276, 366
SspMI	CTAG	6 cut(s)	41, 207, 587, 782, 1077, 1383
StuI	AGGCCT	1 cut(s)	345
StyD4I	CCNGG	1 cut(s)	505
StyI	CCWWGG	4 cut(s)	206, 496, 1153, 1391
TaaI	ACNGT	4 cut(s)	526, 898, 921, 1287
TaiI	ACGT	1 cut(s)	1440
TaqI	TCGA	1 cut(s)	227
TatI	WGTACW	3 cut(s)	1191, 1287, 1466
TauI	GCSGC	1 cut(s)	278
TfiI	GAWTC	5 cut(s)	636, 886, 1041, 1142, 1362
Tru1I	TTAA	4 cut(s)	215, 617, 944, 957
Tru9I	TTAA	4 cut(s)	215, 617, 944, 957
TscAI	CASTG	6 cut(s)	97, 116, 553, 924, 957, 1346
TseFI	GTSAC	2 cut(s)	244, 754
TseI	GCWGC	2 cut(s)	288, 590
Tsp45I	GTSAC	2 cut(s)	244, 754
TspGWI	ACGGA	1 cut(s)	386
TspRI	CASTG	6 cut(s)	97, 116, 553, 924, 957, 1346
Tth111I	GACNNNGTC	1 cut(s)	599
Van91I	CCANNNNNTGG	1 cut(s)	1214
VpaK11BI	GGWCC	3 cut(s)	493, 1386, 1417
VspI	ATTAAT	1 cut(s)	944
XapI	RAATTY	5 cut(s)	8, 99, 132, 833, 1099
XbaI	TCTAGA	1 cut(s)	1076
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1034
XmaJI	CCTAGG	1 cut(s)	206
XspI	CTAG	6 cut(s)	41, 207, 587, 782, 1077, 1383
ZrmI	AGTACT	1 cut(s)	1468

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.