pycom05g25170

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0017115
Physical Location & Seq
Forward (+)
26651392 .. 26653999
2608 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25170.1

Sequence Viewer

Length: 1365 bp
ATGTTGATCCTAGACGGAAAGGGATCCTCTCCAAATCCCTTTCTACCAAATCCACCTAGTTCGAGCAAGAAACTTGGCTTGCAAGTCAGGGTTAAAAGTGGACGACATGATTATGAAGGCCTATCTTACCTCTGCAAAACCTCATTTATCATCATTGACTTGATCAATCTCCGGTGCATTGAGGTCAACCTTGCAGACGAGACAGCTTGGGTTCAGTCAGGAGCCACACTTGGTGAGCTTTATTATAGCATTGCCAAGAAAAGTGGGGTCCATGGATTTCCAGGAGGATTGTGCCCTACTGTTGGTATTGGAGGGCACTTGAGTGGAGGTGGGTTTGGAACCCTAATCAGGAAGCACGGCCTGGCAGCTGATAATGTCGTAGATGCTCGATTCATTGATGTGAATGGAAGAATCCTTGACAGAACAATGGGTGAGGATTTGTTTTGGGCCATCAGAGGAGGTGGAGGATCAAGTTTTGGGGTCATAGTTTCTTGGAAAATCAAATTGATTCAGGTTCCGAAGATCGTGACCGGTTTTACAGTTCACAAAACCCTAGCAGCAGGAGCCGGCAGCCTTGTTCACAGATGGCAATACATTGCAGATAAATTTCACGAGGATCTTTTCATCCGAGTCATAATTCAGAATGTGGGGATTGGGAATCAGAAAACTGTTCAAGCATCTTTCCAATCACTGTTTCTTGGAGGAATTGACAGATTAATGCCACTGATGAAGCTGGGATTGCAGGCAAAAGACTGCATTGAGATGAGTTGGATCCAATCAGCTCAGTACTTTGCTGGATTCCAAAAAGGCCAGCCCTTAGATGTCTTGCTATCTAAAAATCCCCTCTGGAAGATGAATTTCAAAACCAAATCCGACTTTGTGAAGAACCCAATACCCAAACCGGGACTCCAAGGGATTTGGGAGGGGTTTCAGCAGGAACAAAATGTGTACATGATTTTGGATCCATATGGTGGAAGAATGAATGAGATTTCAGAATATGAAACTCCATTTCCTCACAGAAAAGGTAATTTGTTCAACATGCAGTACATAGTGAAGTGGGATGGAAGCGGGGTGGAGGAAACCAACAAACATATCAAATGGATAACAATGCTTTACATATTCATGGGCCCTTATGTTTCCAGGTCTCCAAGGGGTGCCTATATAAATTACAAGGATCTTGATCTGAGTGCCAACAAGGAAGACAACACAAACTTCTTGGAGGCAAGTGCTTGGGGAACAAGGTACTTCAAGGGTAACTTCAAGAGGCTGGCACAAGTGAAGAGCAAGGTTGATCCAGATAACTTCTTCAGAAATGAACAAAGCATTCCTCCTCTTCCTTCTTTGTTTGCAATCACAGAGGTTTGA

Protein Analysis

455

Amino Acids

50.67

Weight (kDa)

9.26

Isoelectric Point (pI)

30.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 24 - 140 1e-18 FAD binding domain
BBE PF08031 386 - 443 1.4e-20 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20860
fragaria_vesca FvH4_3g10830
malus_domestica MD10G1247300.v1.1
prunus_persica Prupe.4G096000_v2.0.a1 Prupe.4G096100_v2.0.a1
pyrus_communis pycom05g25170 pycom10g20520
rosa_chinensis RchiOBHm_Chr5g0017541
rosa_laevigata RLG00000032297
rosa_multiflora Rmu_sc0002565.1_g000003
rosa_roxburghii Rroxscaffold_1G00059900
rosa_rugosa Rorug05G0036800
rosa_wichuraiana Rw5G011300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1154
AccB7I CCANNNNNTGG 1 cut(s) 971
AciI CCGC 1 cut(s) 1068
AcsI RAATTY 2 cut(s) 605, 856
AcuI CTGAAG 1 cut(s) 1291
AdeI CACNNNGTG 1 cut(s) 233
AfaI GTAC 4 cut(s) 788, 950, 1046, 1244
AfiI CCNNNNNNNGG 4 cut(s) 302, 348, 902, 971
AgeI ACCGGT 1 cut(s) 530
AgsI TTSAA 5 cut(s) 674, 862, 1036, 1249, 1261
AjnI CCWGG 3 cut(s) 280, 360, 1139
AjuI GAANNNNNNNTTGG 2 cut(s) 678, 710
AleI CACNNNNGTG 1 cut(s) 321
AluBI AGCT 5 cut(s) 206, 238, 368, 733, 782
AluI AGCT 5 cut(s) 206, 238, 368, 733, 782
Alw26I GTCTC 2 cut(s) 194, 1149
AoxI GGCC 5 cut(s) 118, 358, 447, 808, 1126
ApaI GGGCCC 1 cut(s) 1130
ApeKI GCWGC 3 cut(s) 365, 557, 570
ApoI RAATTY 2 cut(s) 605, 856
AseI ATTAAT 1 cut(s) 716
AsiGI ACCGGT 1 cut(s) 530
AspS9I GGNCC 4 cut(s) 268, 447, 1126, 1127
AsuC2I CCSGG 1 cut(s) 903
AsuHPI GGTGA 2 cut(s) 245, 443
AvaII GGWCC 1 cut(s) 268
BaeGI GKGCMC 3 cut(s) 296, 318, 1130
BamHI GGATCC 3 cut(s) 23, 771, 961
BanI GGYRCC 1 cut(s) 1154
BanII GRGCYC 1 cut(s) 1130
BauI CACGAG 1 cut(s) 611
BbsI GAAGAC 1 cut(s) 1206
BbvI GCAGC 3 cut(s) 377, 569, 582
BccI CCATC 3 cut(s) 458, 579, 1055
BceAI ACGGC 1 cut(s) 373
BciT130I CCWGG 3 cut(s) 282, 362, 1141
BclI TGATCA 1 cut(s) 162
BcnI CCSGG 1 cut(s) 903
BcoDI GTCTC 2 cut(s) 194, 1149
BfaI CTAG 3 cut(s) 11, 57, 554
BisI GCNGC 3 cut(s) 366, 558, 571
BlsI GCNGC 3 cut(s) 367, 559, 572
BmcAI AGTACT 1 cut(s) 788
Bme1390I CCNGG 4 cut(s) 282, 362, 903, 1141
Bme18I GGWCC 1 cut(s) 268
BmgT120I GGNCC 4 cut(s) 268, 447, 1126, 1127
BmrFI CCNGG 4 cut(s) 282, 362, 903, 1141
BmsI GCATC 2 cut(s) 373, 686
BpiI GAAGAC 1 cut(s) 1206
BpuEI CTTGAG 1 cut(s) 340
BpuMI CCSGG 1 cut(s) 903
BsaBI GATNNNNATC 1 cut(s) 1179
BsaI GGTCTC 1 cut(s) 1149
BsaJI CCNNGG 3 cut(s) 271, 910, 1148
BsaWI WCCGGW 2 cut(s) 171, 530
BsaXI ACNNNNNCTCC 2 cut(s) 891, 921
Bsc4I CCNNNNNNNGG 4 cut(s) 302, 348, 902, 971
Bse118I RCCGGY 2 cut(s) 530, 566
Bse3DI GCAATG 2 cut(s) 249, 594
Bse8I GATNNNNATC 1 cut(s) 1179
BseBI CCWGG 3 cut(s) 282, 362, 1141
BseDI CCNNGG 3 cut(s) 271, 910, 1148
BseGI GGATG 2 cut(s) 624, 1066
BseJI GATNNNNATC 1 cut(s) 1179
BseLI CCNNNNNNNGG 4 cut(s) 302, 348, 902, 971
BseMI GCAATG 2 cut(s) 249, 594
BseMII CTCAG 2 cut(s) 797, 1175
BseRI GAGGAG 2 cut(s) 471, 1320
BseSI GKGCMC 3 cut(s) 296, 318, 1130
BseXI GCAGC 3 cut(s) 377, 569, 582
BseYI CCCAGC 1 cut(s) 733
BshFI GGCC 5 cut(s) 120, 360, 449, 810, 1128
BshNI GGYRCC 1 cut(s) 1154
BshTI ACCGGT 1 cut(s) 530
BsiSI CCGG 4 cut(s) 172, 531, 567, 902
BslFI GGGAC 1 cut(s) 918
BslI CCNNNNNNNGG 4 cut(s) 302, 348, 902, 971
BsmAI GTCTC 2 cut(s) 194, 1149
BsmFI GGGAC 1 cut(s) 918
BsmI GAATGC 1 cut(s) 1323
BsnI GGCC 5 cut(s) 120, 360, 449, 810, 1128
Bso31I GGTCTC 1 cut(s) 1149
Bsp120I GGGCCC 1 cut(s) 1126
Bsp1286I GDGCHC 3 cut(s) 296, 318, 1130
Bsp1407I TGTACA 1 cut(s) 948
Bsp19I CCATGG 1 cut(s) 271
BspACI CCGC 1 cut(s) 1068
BspANI GGCC 5 cut(s) 120, 360, 449, 810, 1128
BspCNI CTCAG 2 cut(s) 796, 1176
BspQI GCTCTTC 1 cut(s) 1274
BspT107I GGYRCC 1 cut(s) 1154
BspTNI GGTCTC 1 cut(s) 1149
BsrDI GCAATG 2 cut(s) 249, 594
BsrFI RCCGGY 2 cut(s) 530, 566
BsrGI TGTACA 1 cut(s) 948
BssAI RCCGGY 2 cut(s) 530, 566
BssECI CCNNGG 3 cut(s) 271, 910, 1148
BssSI CACGAG 1 cut(s) 611
BssT1I CCWWGG 3 cut(s) 271, 910, 1148
Bst2BI CACGAG 1 cut(s) 611
Bst2UI CCWGG 3 cut(s) 282, 362, 1141
Bst4CI ACNGT 4 cut(s) 301, 541, 670, 693
Bst6I CTCTTC 2 cut(s) 1274, 1338
BstAUI TGTACA 1 cut(s) 948
BstC8I GCNNGC 5 cut(s) 80, 568, 744, 812, 1269
BstDEI CTNAG 3 cut(s) 783, 817, 1184
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 2 cut(s) 624, 1066
BstMAI GTCTC 2 cut(s) 194, 1149
BstMWI GCNNNNNNNGC 2 cut(s) 563, 739
BstNI CCWGG 3 cut(s) 282, 362, 1141
BstNSI RCATGY 1 cut(s) 1042
BstSCI CCNGG 4 cut(s) 280, 360, 901, 1139
BstSLI GKGCMC 3 cut(s) 296, 318, 1130
BstV1I GCAGC 3 cut(s) 377, 569, 582
BstV2I GAAGAC 1 cut(s) 1206
BstX2I RGATCY 5 cut(s) 23, 616, 771, 961, 1174
BstYI RGATCY 5 cut(s) 23, 616, 771, 961, 1174
BsuRI GGCC 5 cut(s) 120, 360, 449, 810, 1128
BtgI CCRYGG 1 cut(s) 271
BtsCI GGATG 2 cut(s) 624, 1066
BtsIMutI CAGTG 2 cut(s) 689, 722
Cac8I GCNNGC 5 cut(s) 80, 568, 744, 812, 1269
Cfr10I RCCGGY 2 cut(s) 530, 566
Cfr13I GGNCC 4 cut(s) 268, 447, 1126, 1127
Csp6I GTAC 4 cut(s) 787, 949, 1045, 1243
CspAI ACCGGT 1 cut(s) 530
CspCI CAANNNNNGTGG 2 cut(s) 244, 279
CviAII CATG 5 cut(s) 107, 272, 952, 1039, 1123
CviQI GTAC 4 cut(s) 787, 949, 1045, 1243
DdeI CTNAG 3 cut(s) 783, 817, 1184
DraIII CACNNNGTG 1 cut(s) 233
Eam1104I CTCTTC 2 cut(s) 1274, 1338
EarI CTCTTC 2 cut(s) 1274, 1338
Eco130I CCWWGG 3 cut(s) 271, 910, 1148
Eco147I AGGCCT 1 cut(s) 120
Eco24I GRGCYC 1 cut(s) 1130
Eco31I GGTCTC 1 cut(s) 1149
Eco47I GGWCC 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 1291
EcoO109I RGGNCCY 1 cut(s) 1127
EcoRII CCWGG 3 cut(s) 280, 360, 1139
EcoT14I CCWWGG 3 cut(s) 271, 910, 1148
EcoT38I GRGCYC 1 cut(s) 1130
ErhI CCWWGG 3 cut(s) 271, 910, 1148
FaeI CATG 5 cut(s) 110, 275, 955, 1042, 1126
FalI AAGNNNNNCTT 2 cut(s) 1241, 1273
FaqI GGGAC 1 cut(s) 918
FatI CATG 5 cut(s) 106, 271, 951, 1038, 1122
FauI CCCGC 1 cut(s) 1061
FauNDI CATATG 1 cut(s) 967
FbaI TGATCA 1 cut(s) 162
Fnu4HI GCNGC 3 cut(s) 366, 558, 571
FokI GGATG 2 cut(s) 611, 1073
FriOI GRGCYC 1 cut(s) 1130
Fsp4HI GCNGC 3 cut(s) 366, 558, 571
FspBI CTAG 3 cut(s) 11, 57, 554
GluI GCNGC 3 cut(s) 366, 558, 571
GsaI CCCAGC 1 cut(s) 737
HaeIII GGCC 5 cut(s) 120, 360, 449, 810, 1128
HapII CCGG 4 cut(s) 172, 531, 567, 902
Hin1II CATG 5 cut(s) 110, 275, 955, 1042, 1126
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 7 cut(s) 390, 411, 508, 630, 658, 798, 906
HpaII CCGG 4 cut(s) 172, 531, 567, 902
HphI GGTGA 2 cut(s) 245, 443
Hpy166II GTNNAC 5 cut(s) 101, 187, 544, 580, 949
Hpy188I TCNGA 9 cut(s) 455, 519, 629, 642, 663, 874, 994, 1185, 1310
Hpy188III TCNNGA 8 cut(s) 219, 349, 526, 611, 847, 1178, 1261, 1295
Hpy8I GTNNAC 5 cut(s) 101, 187, 544, 580, 949
HpyAV CCTTC 2 cut(s) 110, 1347
HpyCH4III ACNGT 4 cut(s) 301, 541, 670, 693
HpyCH4V TGCA 9 cut(s) 82, 135, 177, 194, 599, 742, 756, 1042, 1349
HpyF10VI GCNNNNNNNGC 2 cut(s) 563, 739
HpyF3I CTNAG 3 cut(s) 783, 817, 1184
Hsp92II CATG 5 cut(s) 110, 275, 955, 1042, 1126
KroI GCCGGC 1 cut(s) 566
KroNI GCCGGC 1 cut(s) 568
Ksp22I TGATCA 1 cut(s) 162
LguI GCTCTTC 1 cut(s) 1274
LmnI GCTCC 2 cut(s) 221, 563
Lsp1109I GCAGC 3 cut(s) 377, 569, 582
LweI GCATC 2 cut(s) 373, 686
MaeI CTAG 3 cut(s) 11, 57, 554
MaeIII GTNAC 2 cut(s) 526, 1253
MboII GAAGA 9 cut(s) 420, 532, 862, 895, 987, 1211, 1291, 1297, 1325
MflI RGATCY 5 cut(s) 23, 616, 771, 961, 1174
MhlI GDGCHC 3 cut(s) 296, 318, 1130
MluCI AATT 7 cut(s) 503, 605, 636, 705, 856, 1027, 1165
MlyI GAGTC 2 cut(s) 639, 900
MmeI TCCRAC 2 cut(s) 749, 897
MroNI GCCGGC 1 cut(s) 566
MseI TTAA 2 cut(s) 93, 716
MslI CAYNNNNRTG 5 cut(s) 111, 321, 398, 761, 1121
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 4 cut(s) 172, 531, 567, 902
MspR9I CCNGG 4 cut(s) 282, 362, 903, 1141
Mva1269I GAATGC 1 cut(s) 1323
MvaI CCWGG 3 cut(s) 282, 362, 1141
MwoI GCNNNNNNNGC 2 cut(s) 563, 739
NaeI GCCGGC 1 cut(s) 568
NciI CCSGG 1 cut(s) 903
NcoI CCATGG 1 cut(s) 271
NdeI CATATG 1 cut(s) 967
NgoMIV GCCGGC 1 cut(s) 566
NlaIII CATG 5 cut(s) 110, 275, 955, 1042, 1126
NmuCI GTSAC 1 cut(s) 526
NspI RCATGY 1 cut(s) 1042
OliI CACNNNNGTG 1 cut(s) 321
PceI AGGCCT 1 cut(s) 120
PciSI GCTCTTC 1 cut(s) 1274
PctI GAATGC 1 cut(s) 1323
PdiI GCCGGC 1 cut(s) 568
PfeI GAWTC 5 cut(s) 390, 411, 508, 658, 798
PflMI CCANNNNNTGG 1 cut(s) 971
PfoI TCCNGGA 1 cut(s) 280
PinAI ACCGGT 1 cut(s) 530
PkrI GCNGC 3 cut(s) 367, 559, 572
PleI GAGTC 2 cut(s) 638, 900
PpsI GAGTC 2 cut(s) 638, 900
PshBI ATTAAT 1 cut(s) 716
Psp6I CCWGG 3 cut(s) 280, 360, 1139
PspFI CCCAGC 1 cut(s) 733
PspGI CCWGG 3 cut(s) 280, 360, 1139
PspOMI GGGCCC 1 cut(s) 1126
PspPI GGNCC 4 cut(s) 268, 447, 1126, 1127
PsuI RGATCY 5 cut(s) 23, 616, 771, 961, 1174
PvuII CAGCTG 1 cut(s) 368
RsaI GTAC 4 cut(s) 788, 950, 1046, 1244
RsaNI GTAC 4 cut(s) 787, 949, 1045, 1243
RseI CAYNNNNRTG 5 cut(s) 111, 321, 398, 761, 1121
SapI GCTCTTC 1 cut(s) 1274
SaqAI TTAA 2 cut(s) 93, 716
SatI GCNGC 3 cut(s) 366, 558, 571
Sau96I GGNCC 4 cut(s) 268, 447, 1126, 1127
ScaI AGTACT 1 cut(s) 788
SchI GAGTC 2 cut(s) 639, 900
ScrFI CCNGG 4 cut(s) 282, 362, 903, 1141
SduI GDGCHC 3 cut(s) 296, 318, 1130
SfaNI GCATC 2 cut(s) 373, 686
SinI GGWCC 1 cut(s) 268
SmiMI CAYNNNNRTG 5 cut(s) 111, 321, 398, 761, 1121
SmlI CTYRAG 1 cut(s) 319
SmoI CTYRAG 1 cut(s) 319
Sse9I AATT 7 cut(s) 503, 605, 636, 705, 856, 1027, 1165
SseBI AGGCCT 1 cut(s) 120
SsiI CCGC 1 cut(s) 1068
SspMI CTAG 3 cut(s) 11, 57, 554
StuI AGGCCT 1 cut(s) 120
StyD4I CCNGG 4 cut(s) 280, 360, 901, 1139
StyI CCWWGG 3 cut(s) 271, 910, 1148
TaaI ACNGT 4 cut(s) 301, 541, 670, 693
TaqI TCGA 2 cut(s) 62, 388
TasI AATT 7 cut(s) 503, 605, 636, 705, 856, 1027, 1165
TatI WGTACW 3 cut(s) 786, 948, 1044
TfiI GAWTC 5 cut(s) 390, 411, 508, 658, 798
Tru1I TTAA 2 cut(s) 93, 716
Tru9I TTAA 2 cut(s) 93, 716
TscAI CASTG 2 cut(s) 696, 729
TseFI GTSAC 1 cut(s) 526
TseI GCWGC 3 cut(s) 365, 557, 570
Tsp45I GTSAC 1 cut(s) 526
TspDTI ATGAA 9 cut(s) 129, 382, 613, 743, 869, 995, 1014, 1111, 1329
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 2 cut(s) 696, 729
Van91I CCANNNNNTGG 1 cut(s) 971
VpaK11BI GGWCC 1 cut(s) 268
VspI ATTAAT 1 cut(s) 716
XapI RAATTY 2 cut(s) 605, 856
XceI RCATGY 1 cut(s) 1042
XspI CTAG 3 cut(s) 11, 57, 554
ZrmI AGTACT 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.